Chip carrier device for biochemical synthesis and biochemical synthesis system

Abstract

The utility model discloses a chip carrier device for biochemical synthesis and a biochemical synthesis system. The chip carrying table device comprises a carrying table main body which is provided with a cavity with a downward opening, the top of the cavity is provided with a chip installation area, and the chip installation area is provided with a plurality of through holes; the carrying table support is positioned below the carrying table main body; the carrying table support is connected with the carrying table main body through a rotating pin; an angle adjusting assembly is arranged on the carrying table support, and the carrying table body has the freedom degree of horizontally rotating around the center of the rotating pin under the adjusting effect of the angle adjusting assembly. A sealing assembly matched with the cavity is arranged on the carrying table support; a negative pressure adsorption assembly is arranged below the sealing assembly and communicates with the cavity through a pipeline. And the cavity forms a negative pressure environment facing the plurality of through holes under the action of the negative pressure adsorption assembly so as to adsorb the chip covering the chip mounting area. The device can meet different requirements of positioning, position adjusting, fixing and the like of the chip, and precise positioning and precise sealing of the chip are achieved.

Description

Technical Field

[0001] This disclosure relates to the field of biochemical synthesis technology, and in particular to a chip platform device and a biochemical synthesis system for biochemical synthesis. Background Technology

[0002] The technology of synthesizing DNA base chains on chip surfaces mainly involves four chemical reactions: coupling, capping, oxidation, and deprotection. Coupling is usually achieved through inkjet printing, while capping, oxidation, and deprotection require other fluid control devices. Chip positioning, fixation, and handling of reagent leakage during the fluid reaction on the chip surface are all crucial. Chip positioning directly affects the accuracy of camera alignment, while chip fixation directly affects the landing point of the inkjet printing ink. If the base ink cannot repeatedly land on the physical reaction site or the deviation is too large, the reaction process is likely to fail or cause contamination. Therefore, the chip needs to remain in a constant position or be controlled within acceptable deviations throughout the synthesis process.

[0003] After the coupling reaction is completed via inkjet printing, it is generally completed using a fluid device. This fluid device is typically a pressing mechanism that presses against the chip surface to form a sealed reaction chamber. Different reagents are then introduced into the reaction chamber to carry out reactions such as capping, oxidation, and deprotection. During these reactions, if the pressing mechanism malfunctions and fails to seal, reagents will leak. Leakage poses significant risks: firstly, it affects the reaction process; secondly, the leaked reagents are often highly corrosive and can severely damage equipment components. Therefore, leak prevention is crucial. Furthermore, high-throughput chip synthesis typically involves large chips, which are subjected to considerable pressure during pressing. If the stage flatness is insufficient, the chips are highly susceptible to crushing. Therefore, the stage surface must have sufficient precision to accommodate the high pressure exerted by the chip.

[0004] Most of the devices disclosed in the existing technology are traditional negative pressure adsorption platforms or pressure plate structures, which cannot meet the positioning accuracy and sealing accuracy required in DNA biosynthesis. Utility Model Content

[0005] In view of this, the present disclosure provides a chip stage device and a biochemical synthesis system for biochemical synthesis, which at least partially solves the problems in the prior art that cannot guarantee the positional accuracy and sealing accuracy of the chip in DNA biosynthesis.

[0006] In a first aspect, embodiments of this disclosure provide a chip stage device for biochemical synthesis, comprising:

[0007] The stage body has a downward-facing chamber, and the top of the chamber has a chip mounting area with several through holes.

[0008] A platform support is disposed below the platform body; the platform support is connected to the platform body by a rotating pin; an angle adjustment component is provided on the platform support, and the platform body has the freedom to rotate horizontally around the center of the rotating pin under the adjustment of the angle adjustment component.

[0009] The platform support is provided with a sealing assembly that matches the chamber;

[0010] A negative pressure adsorption component is installed below the sealing component, and the negative pressure adsorption component is connected to the chamber through a pipeline; the chamber forms a negative pressure environment facing several through holes under the action of the negative pressure adsorption component, so as to adsorb the chip covering the chip mounting area.

[0011] Optionally, the sealing assembly includes a sealing plate and a sealing gasket disposed on the sealing plate;

[0012] The periphery of the sealing gasket is abutted against the periphery of the chamber;

[0013] The sealing plate has several through holes;

[0014] The negative pressure adsorption assembly includes a power component, an air collection drain connected to the power output end of the power component, and several air pipes, each of which is independently configured. One end of each of the air pipes is connected to a different outlet of the air collection drain, and the other end extends through several through holes to the chamber.

[0015] Optionally, the power component includes a vacuum pump and a vacuum breaker valve, with one end of the vacuum breaker valve connected to the vacuum pump and the other end open to the environment.

[0016] Optionally, the platform support includes a first support frame and a second support frame. The first support frame includes a first flat plate and a first branch located below the first flat plate. The first flat plate has a first pin hole that matches the rotating pin. The platform body has a second pin hole that matches the first pin hole.

[0017] The second support frame includes a second flat plate portion and a second branch portion located below the second flat plate portion; one side of the sealing plate is located on the first flat plate portion, and the other side is located on the second flat plate portion;

[0018] The angle adjustment assembly includes a first angle adjustment plate and a second angle adjustment plate. The first angle adjustment plate is installed on a first side of the second flat plate, and the second angle adjustment plate is installed on a second side of the second flat plate.

[0019] The first angle adjustment plate has a first adjustment hole for accommodating the first set screw, and the top end of the first set screw abuts against one side of the platform body;

[0020] The second angle adjustment plate has a second adjustment hole for accommodating the second set screw, and the second adjustment hole is corresponding to the first adjustment hole; the top end of the second set screw abuts against the other side of the platform body;

[0021] The first set screw has a degree of freedom to move along the first adjustment hole; the second set screw has a degree of freedom to move along the second adjustment hole.

[0022] Optionally, the second angle adjustment plate has the same structure as the first angle adjustment plate;

[0023] The distance from the rotating pin to the inner side of the first angle adjusting plate is equal to the distance from the rotating pin to the inner side of the second angle adjusting plate;

[0024] The length between one side of the sealing plate on the first plate portion and the other side of the sealing plate on the second plate portion is H1, the distance between the inner side of the first plate portion and the inner side of the second plate portion is H2, and the distance between the outer side of the first plate portion and the outer side of the second plate portion is H3, where H2 < H1 < H3.

[0025] Optionally, the platform support includes a support plate and a support bracket fixed below the support plate. The support plate has a through hole, the area of ​​which is smaller than the area of ​​the sealing plate.

[0026] The support plate has a first pin hole that matches the rotating pin, and the platform body has a second pin hole that matches the first pin hole;

[0027] The angle adjustment assembly includes a first angle adjustment plate and a second angle adjustment plate respectively installed on a first side and a second side of the support plate; the distance from the rotating pin to the inner side of the first angle adjustment plate is equal to the distance from the rotating pin to the inner side of the second angle adjustment plate;

[0028] The first angle adjustment plate has a first adjustment hole for accommodating a first set screw, and the top end of the first set screw abuts against one side of the stage body; the second angle adjustment plate has a second adjustment hole for accommodating a second set screw, and the second adjustment hole is correspondingly arranged with the first adjustment hole; the top end of the second set screw abuts against the other side of the stage body.

[0029] The first set screw has a degree of freedom to move along the first adjustment hole; the second set screw has a degree of freedom to move along the second adjustment hole.

[0030] Optionally, a plurality of the via arrays are provided.

[0031] Optionally, the chip mounting area has a retaining edge on its periphery, and the retaining edge forms a groove with the chip mounting area;

[0032] The chip stage device also includes a waste discharge component, which includes a negative pressure pump and a storage bottle. The negative pressure pump is connected to the groove through a pipeline.

[0033] Optionally, a liquid detection sensor is installed at the end of the pipe located within the groove;

[0034] The chip platform device also includes a central control center, and the negative pressure pump and the liquid detection sensor are both connected to the central control center via signal. The central control center controls the negative pressure pump to start pumping away waste liquid based on the alarm information from the liquid detection sensor.

[0035] Secondly, this application discloses a biochemical synthesis system, including the aforementioned chip stage device for biochemical synthesis and a chip disposed on the chip stage device.

[0036] The chip stage device for biochemical synthesis disclosed in this application creates a negative pressure environment within the chamber through a negative pressure adsorption component. This effectively adsorbs the chip covering the chip mounting area, ensuring the chip remains stable during DNA biosynthesis (i.e., biochemical synthesis) and avoiding experimental errors caused by chip movement or vibration. The stage body and stage support are connected by a rotating pin and equipped with an angle adjustment component, allowing the stage body to rotate horizontally around the center of the rotating pin. This design allows the chip to be adjusted to the optimal angle to optimize the DNA synthesis process and adapt to different experimental needs. The stage support is equipped with a sealing component that matches the chamber, ensuring the chamber's airtightness. The closed design prevents external contaminants from entering the chamber and affecting the purity and accuracy of DNA synthesis, while also ensuring the stability of negative pressure adsorption and maintaining the chip's position. The stage support is connected to the main body of the stage via a simple and stable rotating pin. The angle adjustment component is rationally designed and easy to operate. This structure not only improves the practicality of the device but also facilitates user adjustments and maintenance. Angle adjustment ensures the chip is accurately repositioned on the stage. Furthermore, this design takes into account chips of different sizes and types. By adjusting the geometry of the chamber and the distribution of through holes, it can adapt to the needs of various biochips, further enhancing the device's versatility and practicality.

[0037] The above description is merely an overview of the technical solution disclosed herein. In order to better understand the technical means of this disclosure and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0038] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0039] Figure 1 This is a perspective view of a specific embodiment of the chip stage device for biochemical synthesis disclosed in this application.

[0040] Figure 2 for Figure 1 Exploded view.

[0041] Figure 3 for Figure 2 A diagram from another angle.

[0042] Figure 4 This is a schematic diagram of a first embodiment of the platform support in this application.

[0043] Figure 5 This is a schematic diagram of a second embodiment of the platform support in this application.

[0044] Explanation of reference numerals in the attached figures:

[0045] 100. Main body of the platform; 101. Chamber;

[0046] 200. Platform support;

[0047] 210. First support frame; 211. First flat plate section; 212. First support section;

[0048] 220. Second support frame; 221. Second flat plate section; 222. Second branch;

[0049] 230. Load-bearing plate; 240. Load-bearing bracket;

[0050] 310. Sealing plate; 320. Sealing gasket;

[0051] 400. Angle adjustment assembly; 410. First angle adjustment plate; 420. Second angle adjustment plate;

[0052] 500, Rotary pin;

[0053] 600. Negative pressure adsorption component. Detailed Implementation

[0054] The present disclosure will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the disclosure. Furthermore, it should be noted that, for ease of description, only the parts relevant to the present disclosure are shown in the accompanying drawings.

[0055] It should be noted that, where there is no conflict, the embodiments and features described in this disclosure can be combined with each other. The technical solutions of this disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0056] Unless otherwise stated, the exemplary implementations / embodiments shown are to be understood as providing exemplary features of various details that provide ways in which the technical concepts of this disclosure can be implemented in practice. Therefore, unless otherwise stated, the features of various implementations / embodiments may be additionally combined, separated, interchanged and / or rearranged without departing from the technical concepts of this disclosure.

[0057] The use of crosshairs and / or shading in the accompanying drawings is generally used to clarify the boundaries between adjacent components. Thus, unless otherwise stated, the presence or absence of crosshairs or shading does not convey or indicate any preference or requirement for the specific material, material properties, dimensions, proportions, commonalities between the illustrated components, or any other characteristics, properties, etc., of the components. Furthermore, in the accompanying drawings, the dimensions and relative dimensions of components may be exaggerated for clarity and / or descriptive purposes. When exemplary embodiments can be implemented differently, a specific process sequence may be performed in a different order than that described. For example, two consecutively described processes may be performed substantially simultaneously or in the reverse order of their description. Furthermore, the same reference numerals denote the same components.

[0058] When a component is referred to as being "on" or "above" another component, "connected to," or "joined to" another component, the component may be directly on, directly connected to, or directly joined to the other component, or there may be intermediate components. However, when a component is referred to as being "directly on" another component, "directly connected to," or "directly joined to" another component, there are no intermediate components. Therefore, the term "connection" can refer to a physical connection, an electrical connection, etc., and may or may not have intermediate components.

[0059] For descriptive purposes, this disclosure may use spatial relative terms such as “below,” “under,” “below,” “down,” “above,” “above,” “higher,” and “side (e.g., in a “sidewall”)” to describe the relationship between one component and another component as shown in the accompanying drawings. In addition to the orientations depicted in the drawings, the spatial relative terms are also intended to encompass different orientations of the device during use, operation, and / or manufacture. For example, if the device in the drawings is flipped, a component described as “below” or “under” another component or feature would subsequently be positioned “above” said other component or feature. Thus, the exemplary term “below” can encompass both “above” and “below” orientations. Furthermore, the device may be otherwise positioned (e.g., rotated 90 degrees or in other orientations), thus interpreting the spatial relative descriptive terms used herein accordingly.

[0060] The terminology used herein is for the purpose of describing particular embodiments and is not intended to be limiting. As used herein, unless the context clearly indicates otherwise, the singular forms “a” and “the” are intended to include the plural forms as well. Furthermore, when the terms “comprising” and / or “including” and variations thereof are used in this specification, it indicates the presence of the stated features, integrals, steps, operations, parts, components, and / or groups thereof, but does not exclude the presence or addition of one or more other features, integrals, steps, operations, parts, components, and / or groups thereof. It should also be noted that, as used herein, the terms “substantially,” “about,” and other similar terms are used as approximate terms rather than as terms of degree, thus explaining the inherent biases in measurements, calculated values, and / or provided values ​​that would be recognized by one of ordinary skill in the art.

[0061] Reference Figure 1 and Figure 2 This application discloses a chip stage device for biochemical synthesis, including: a stage body 100 and a stage support 200 disposed below the stage body 100. The stage support 200 is provided with a sealing component that matches the chamber 101, and a negative pressure adsorption component 600 for providing negative pressure is installed below the sealing component.

[0062] The stage body 100 has a downward-facing chamber 101, and the top of the chamber 101 has a chip mounting area with several through holes.

[0063] The stage support 200 is connected to the stage body 100 via a rotating pin 500. An angle adjustment component 400 is provided on the stage support 200. Under the adjustment of the angle adjustment component 400, the stage body 100 has the freedom to rotate horizontally around the center of the rotating pin 500, so that the angle of the chip can be flexibly adjusted to adapt to different experimental needs, thereby improving the flexibility and versatility of the device.

[0064] The design of the stage body 100 and stage support 200 ensures that the stage surface has sufficient flatness and precision, which can be adapted to high-throughput synthesis chips that can withstand large pressure. It can effectively avoid the problem of chips being crushed due to uneven pressure during the pressing process, and ensure the integrity of the chips and the success rate of the experiment.

[0065] The negative pressure adsorption component 600 is connected to the chamber 101 through a pipeline; under the action of the negative pressure adsorption component 600, the chamber 101 forms a negative pressure environment facing several through holes to adsorb the chips covered on the chip mounting area.

[0066] Specifically, when the chip covers the upper surface of the chip mounting area, the chamber 101 under the lower sealing component forms a sealed chamber 101. Therefore, the negative pressure provided to the sealed chamber 101 by the negative pressure adsorption component 600 can be applied to the chip through several through holes, thereby firmly adsorbing the chip onto the upper surface of the chip mounting area and achieving effective fixation of the chip.

[0067] The negative pressure environment provided by the negative pressure adsorption component 600 allows the sealed chamber 101 to maintain its airtightness, preventing leakage of highly corrosive reagents, protecting the integrity of equipment components, and improving the service life and safety of the equipment.

[0068] In this embodiment, a plurality of through holes are preferably arranged in an array to form a uniform adsorption force on the chip, thereby achieving uniform negative pressure adsorption on the chip without damaging it. This ensures the accurate landing position of the inkjet printing ink and effectively reduces reaction failure or contamination problems caused by positional deviation.

[0069] Furthermore, the chip mounting area has a positioning edge on its periphery. Through tolerance control, after the chip is placed within the positioning edge, it can be limited to the calibration range allowed by the alignment camera. The stage body 100 is preferably made of 316L stainless steel, which is high in strength and corrosion-resistant, ensuring that the chip can maintain its shape stability and not deform when subjected to pressure, thus ensuring a high degree of flatness.

[0070] The sealing assembly includes a sealing plate 310 and a sealing gasket 320 disposed on the sealing plate 310. The periphery of the sealing gasket 320 is abutted against the periphery of the chamber 101 for sealing the chamber 101 from below in the assembled state.

[0071] The sealing plate 310 has several through holes, all of which are inside the hollow part of the sealing gasket 320.

[0072] Reference Figure 2 and Figure 3 The negative pressure adsorption component 600 includes a power component, an air collection manifold connected to the power output end of the power component, and several air pipes, which are independently arranged. One end of each air pipe is connected to a different outlet of the air collection manifold, and the other end extends through several through holes to the chamber 101. The number of air pipes is consistent with the number of through holes, which satisfies the negative pressure function and ensures the sealing effect of the chamber 101.

[0073] The power components include a vacuum pump and a vacuum breaker valve. One end of the vacuum breaker valve is connected to the vacuum pump, and the other end is open to the environment. When it is necessary to fix the chip, i.e. when negative pressure is required, the vacuum pump starts, and the vacuum breaker valve is connected to the vacuum pump. The vacuum pump starts to extract air from the sealed chamber 101 through the gas collector and several gas pipes to form a negative pressure environment. At this time, the vacuum breaker valve is connected to the vacuum pump to ensure that the vacuum pump can effectively extract gas and maintain negative pressure in the chamber 101.

[0074] During vacuum pump operation, the vacuum breaker valve remains connected to the vacuum pump to prevent external air from entering chamber 101 and maintain a negative pressure state inside chamber 101. The negative pressure environment acts on the chip covered on the chip mounting area through several through holes, making it firmly adsorbed on the stage and ensuring the stability of the chip position during DNA biosynthesis.

[0075] When it is necessary to break the negative pressure environment, the vacuum pump stops working, and the vacuum breaking valve switches to the state of being in communication with the environment, allowing external air to enter the chamber 101, quickly neutralizing the negative pressure in the chamber 101, and releasing the chip from the adsorption state. This process can be achieved by controlling the opening and closing state of the vacuum breaking valve, which is simple and quick to operate.

[0076] Reference Figure 4 In the first embodiment, the platform support 200 includes a first support frame 210 and a second support frame 220. The first support frame 210 includes a first flat plate portion 211 and a first branch portion 212 located below the first flat plate portion 211. The first flat plate portion 211 has a first pin hole that matches the rotating pin 500. The platform body 100 has a second pin hole that matches the first pin hole, ensuring that after the platform body 100 and the platform support 200 are assembled, the second pin hole is aligned with the first pin hole.

[0077] The second support frame 220 includes a second flat plate portion 221 and a second branch portion 222 located below the second flat plate portion 221; one side of the sealing plate 310 is located on the first flat plate portion 211, and the other side is located on the second flat plate portion 221.

[0078] Specifically, the length between one side of the sealing plate 310 on the first plate portion 211 and the other side of the sealing plate 310 on the second plate portion 221 is H1, the distance between the inner side of the first plate portion 211 and the inner side of the second plate portion 221 is H2, and the distance between the outer side of the first plate portion 211 and the outer side of the second plate portion 221 is H3, where H2 < H1 < H3, ensuring that the independently installed first support frame 210 and second support frame 220 have good load-bearing stability.

[0079] The angle adjustment assembly 400 includes a first angle adjustment plate 410 and a second angle adjustment plate 420. The first angle adjustment plate 410 is installed on the first side of the second flat plate portion 221, and the second angle adjustment plate 420 is installed on the second side of the second flat plate portion 221. The first angle adjustment plate 410 has a first adjustment hole for accommodating a first set screw, and the top end of the first set screw abuts against one side of the platform body 100.

[0080] The second angle adjustment plate 420 has a second adjustment hole for accommodating the second set screw, and the second adjustment hole is corresponding to the first adjustment hole; the top of the second set screw is pressed against the other side of the platform body 100.

[0081] When assembled, the stability of the platform body 100 can be ensured by the first set screw that abuts against one side of the platform body 100 and the second set screw that abuts against the other side of the platform body 100.

[0082] In this embodiment, the first set screw has the degree of freedom to move along the first adjustment hole; the second set screw has the degree of freedom to move along the second adjustment hole. Specifically, when the chip needs to be positioned, that is, when the stage body 100 needs to be positioned, for example, when clockwise adjustment is required, the first set screw is controlled to move outward (i.e., move away from the stage body 100), and the second set screw is controlled to move inward (i.e. move closer to the stage body 100). Under the thrust of the second set screw, the stage body 100 will rotate clockwise around the center of the rotating pin 500. When the horizontal rotation is in place, the top ends of the first set screw and the second set screw are respectively pressed against the two sides of the stage body 100.

[0083] When counterclockwise adjustment is required, the second setter is controlled to move outward (i.e., move away from the main body 100 of the platform) and the first setter is controlled to move inward (i.e. move closer to the main body 100 of the platform). Under the thrust of the first setter, the main body 100 of the platform will rotate counterclockwise around the center of the rotating pin 500. When the horizontal rotation is in place, the tops of the first setter and the second setter are respectively pressed against the two sides of the main body 100 of the platform.

[0084] In this embodiment, the second angle adjustment plate 420 has the same structure as the first angle adjustment plate 410, which facilitates processing.

[0085] The distance from the rotating pin 500 to the inner side of the first angle adjusting plate 410 is equal to the distance from the rotating pin 500 to the inner side of the second angle adjusting plate 420, ensuring precise control of the rotation angle.

[0086] Reference Figure 5 In the second embodiment, the platform support 200 includes a support plate 230 and a support bracket 240 fixed below the support plate 230. The support plate 230 has a through hole, the area of ​​which is smaller than the area of ​​the sealing plate 310. The support plate 230 has a first pin hole that matches the rotating pin 500, and the platform body 100 has a second pin hole that matches the first pin hole.

[0087] The angle adjustment assembly 400 includes a first angle adjustment plate 410 and a second angle adjustment plate 420 respectively installed on the first side and the second side of the support plate 230; the distance from the rotating pin 500 to the inner side of the first angle adjustment plate 410 is equal to the distance from the rotating pin 500 to the inner side of the second angle adjustment plate 420.

[0088] The first angle adjustment plate 410 has a first adjustment hole for accommodating the first set screw, and the top end of the first set screw abuts against one side of the stage body 100; the second angle adjustment plate 420 has a second adjustment hole for accommodating the second set screw, and the second adjustment hole is corresponding to the first adjustment hole; the top end of the second set screw abuts against the other side of the stage body 100.

[0089] The first set screw has a degree of freedom to move along the first adjustment hole; the second set screw has a degree of freedom to move along the second adjustment hole.

[0090] Furthermore, the chip mounting area has a retaining edge on its periphery, which forms a groove with the chip mounting area to store leaked reagents.

[0091] The chip stage device also includes a waste discharge component, which includes a negative pressure pump and a storage bottle. The negative pressure pump is connected to the groove through a pipeline, and the waste liquid in the groove can be drawn out to the storage bottle through the pipeline under the negative pressure of the negative pressure pump.

[0092] Furthermore, a liquid detection sensor is installed at the end of the pipe located in the groove to detect liquid, and an alarm is triggered when liquid is detected.

[0093] The chip platform device also includes a central control center. The negative pressure pump and liquid detection sensor are all connected to the central control center. The central control center controls the negative pressure pump to start pumping away waste liquid based on the alarm information of the liquid detection sensor. That is, when the liquid detection sensor detects liquid, it triggers an alarm and then immediately starts the negative pressure pump to pump away the liquid.

[0094] In this embodiment, all parts in contact with liquid are made of materials resistant to corrosion by all reagents, thereby improving the service life of the device.

[0095] The chip stage device for biochemical synthesis disclosed in this application creates a negative pressure environment within the chamber through a negative pressure adsorption component. This effectively adsorbs the chip covering the chip mounting area, ensuring the chip remains stable during DNA biosynthesis and avoiding experimental errors caused by chip movement or vibration. The stage body and stage support are connected by a rotating pin and equipped with an angle adjustment component, allowing the stage body to rotate horizontally around the center of the rotating pin. This design allows the chip to be adjusted to the optimal angle to optimize the DNA synthesis process and adapt to different experimental needs. The stage support is equipped with a sealing component that matches the chamber, ensuring the chamber's airtightness and preventing... External contaminants entering the chamber can affect the purity and accuracy of DNA synthesis, while ensuring the stability of negative pressure adsorption and maintaining the chip's position. The stage support is connected to the main body of the stage via a simple and stable rotating pin. The angle adjustment component is rationally designed and easy to operate. This structure not only improves the practicality of the device but also facilitates user adjustments and maintenance. Angle adjustment ensures the chip is accurately repositioned on the stage. Furthermore, this design takes into account chips of different sizes and types. By adjusting the geometry of the chamber and the distribution of through holes, it can adapt to the needs of various biochips, further enhancing the device's versatility and practicality.

[0096] In summary, this chip stage device for biochemical synthesis integrates functions such as positioning, fixation, leakage alarm, waste liquid collection, pressure resistance, and corrosion resistance, ensuring the stability and safety of the chip during the synthesis process. Through its advantages of stable adsorption, flexible adjustment, superior sealing, high-precision stage surface, convenient operation, and strong versatility, it can significantly improve the efficiency and success rate of DNA synthesis experiments, representing a significant technological advancement in the field of biosynthesis.

[0097] Secondly, this application discloses a biochemical synthesis system, including the aforementioned chip stage device for biochemical synthesis and a chip disposed on the chip stage device. Through the chip stage device, the chip can be positioned precisely by adjusting its position, which ensures the accuracy of camera alignment. The chip is reliably fixed by negative pressure adsorption, ensuring the accurate landing position of inkjet printing ink. The basic ink can repeatedly land on the physical reaction site, ensuring the reaction effect.

[0098] After the coupling reaction is completed by inkjet printing, it is generally completed by a fluid device, which is usually a pressing mechanism that presses against the chip surface to form a sealed reaction chamber. Different reagents are then introduced into the reaction chamber to carry out reactions such as capping, oxidation, and deprotection. During these reactions, the chip stage device can effectively seal the chip to ensure that the reagents do not leak.

[0099] In addition, the chip stage device can provide excellent flatness, ensuring that the chip will not be damaged even under great pressure during pressing, effectively guaranteeing the accuracy and reliability of DNA chip synthesis.

[0100] In the description of this specification, the references to terms such as "one embodiment / mode," "some embodiments / modes," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment / mode or example is included in at least one embodiment / mode or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment / mode or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments / modes or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments / modes or examples described in this specification, as well as the features of different embodiments / modes or examples.

[0101] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0102] Those skilled in the art should understand that the above embodiments are merely for illustrating the present disclosure and are not intended to limit the scope of the disclosure. Those skilled in the art can make other changes or modifications based on the above disclosure, and these changes or modifications still fall within the scope of the present disclosure.

Claims

1. A chip stage apparatus for biochemical synthesis, characterized by, The utility model relates to a chip mounting device, including: The platform main part is opened with the chamber that opens down, and the chamber top has the chip mounting area that opens with a plurality of through -holes; The platform support is set up below the platform main part, and the platform support is connected with the platform main part through the rotating pin, and the angle adjusting assembly is set up on the platform support, and the platform main part has the freedom of horizontal rotation around the center of the rotating pin under the adjusting effect of the angle adjusting assembly; The sealing assembly that matches with the chamber is set up on the platform support; The negative pressure adsorption assembly is equipped below the sealing assembly, and the negative pressure adsorption assembly communicates with the chamber through the pipeline; the chamber forms the negative pressure environment that meets a plurality of through -holes under the action of the negative pressure adsorption assembly to adsorb the chip covered on the chip mounting area.

2. The chip stage apparatus for biochemical synthesis according to claim 1, wherein, The sealing assembly includes a sealing plate and a sealing gasket disposed on the sealing plate; The peripheral side of the sealing gasket is in close contact with the peripheral side of the chamber; A plurality of through holes are formed in the sealing plate; The negative pressure adsorption assembly includes a power unit, a gas collection and exhaust connected to the power output end of the power unit, and a plurality of air pipe lines, wherein the plurality of air pipe lines are independently arranged; one end of the plurality of air pipe lines is respectively connected to different exhaust ports of the gas collection and exhaust, and the other end respectively penetrates through the plurality of through holes and extends into the chamber.

3. The chip stage apparatus for biochemical synthesis according to claim 2, wherein, The power unit includes a vacuum pump and a vacuum breaking valve, one end of the vacuum breaking valve is connected to the vacuum pump, and the other end is communicated with the environment.

4. The chip stage apparatus for biochemical synthesis according to claim 2, wherein, The platform support includes a first support frame and a second support frame, the first support frame includes a first flat plate portion and a first support portion located below the first flat plate portion, a first pin hole matched with the rotating pin is formed in the first flat plate portion; a second pin hole matched with the first pin hole is formed in the platform main body; The second support frame includes a second flat plate portion and a second support portion located below the second flat plate portion; one side of the sealing plate is located on the first flat plate portion, and the other side is located on the second flat plate portion; The angle adjusting assembly includes a first angle adjusting plate and a second angle adjusting plate, the first angle adjusting plate is installed on the first side of the second flat plate portion, and the second angle adjusting plate is installed on the second side of the second flat plate portion; A first adjusting hole for accommodating a first top wire is formed in the first angle adjusting plate, and the top end of the first top wire is in close contact with one side of the platform main body; A second adjusting hole for accommodating a second top wire is formed in the second angle adjusting plate, and the second adjusting hole is correspondingly arranged with the first adjusting hole; the top end of the second top wire is in close contact with the other side of the platform main body; The first top wire has the freedom of moving along the first adjusting hole; the second top wire has the freedom of moving along the second adjusting hole.

5. The chip stage apparatus for biochemical synthesis according to claim 4, wherein, The second angle adjusting plate is arranged in the same structure as the first angle adjusting plate; The distance from the rotating pin to the inner side of the first angle adjusting plate is equal to the distance from the rotating pin to the inner side of the second angle adjusting plate; A length between one side of the sealing plate on the first flat plate part and the other side of the sealing plate on the second flat plate part is H1, a distance between an inner side of the first flat plate part and an inner side of the second flat plate part is H2, and a distance between an outer side of the first flat plate part and an outer side of the second flat plate part is H3, H2 < H1 < H3.

6. The chip stage apparatus for biochemical synthesis according to claim 2, wherein, The support includes a bearing plate and a bearing support fixed below the bearing plate, the bearing plate is provided with a through hole, and an area of the through hole is smaller than an area of the sealing plate; The bearing plate is provided with a first pin hole matched with the rotating pin, and the support body is provided with a second pin hole matched with the first pin hole; The angle adjusting assembly includes first and second angle adjusting plates respectively installed on first and second side portions of the bearing plate, and a distance from the rotating pin to an inner side of the first angle adjusting plate is equal to a distance from the rotating pin to an inner side of the second angle adjusting plate; The first angle adjusting plate is provided with a first adjusting hole accommodating a first jackscrew, and a top end of the first jackscrew abuts against one side of the support body; the second angle adjusting plate is provided with a second adjusting hole accommodating a second jackscrew, and the second adjusting hole is arranged correspondingly to the first adjusting hole; and a top end of the second jackscrew abuts against the other side of the support body; The first jackscrew has a freedom of movement along the first adjusting hole, and the second jackscrew has a freedom of movement along the second adjusting hole.

7. The chip stage apparatus for biochemical synthesis according to claim 1, wherein, The through hole array is arranged.

8. The chip stage apparatus for biochemical synthesis according to claim 1, wherein, The chip mounting area has a retaining edge on a circumferential side, and the retaining edge forms a groove with the chip mounting area; The chip support device further includes a waste discharge assembly, and the waste discharge assembly includes a negative pressure pump and a storage bottle, and the negative pressure pump is communicated with the groove through a pipeline.

9. The chip stage apparatus for biochemical synthesis according to claim 8, wherein, An end portion of the pipeline located in the groove is provided with a liquid detection sensor; The chip support device further includes a general control center, and the negative pressure pump and the liquid detection sensor are signal connected with the general control center; and the general control center controls the negative pressure pump to start to draw waste liquid based on alarm information of the liquid detection sensor.

10. A biochemical synthesis system, characterized by, The chip support device for biochemical synthesis includes the chip support device according to any one of claims 1-9 and a chip arranged on the chip support device.

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