Purification device for sephadex

Abstract

The utility model relates to a sephadex purification device which comprises a base and a plurality of chromatographic columns, a supporting frame is arranged on the base, a plurality of grooves used for containing the chromatographic columns are formed in the supporting frame, clamping pieces used for clamping the chromatographic columns are arranged in the grooves, and the upper ends and the lower ends of the chromatographic columns are provided with liquid injection ports and liquid discharge ports; a liquid supply mechanism is arranged above the supporting frame, a collection mechanism is arranged on the base, the injection process of eluent and the collection process of samples can be more convenient and faster through the design of the liquid supply mechanism and the collection mechanism, and the operation process is simplified. The device ensures that the eluent can be uniformly injected into the chromatographic column and the separated sample can be accurately collected through an accurate liquid supply and collection system. Therefore, experimental errors can be reduced, and the accuracy and reliability of the experiment can be improved.

Description

Technical Field

[0001] This application relates to the field of dextran gel purification technology, and in particular to a purification apparatus for dextran gel. Background Technology

[0002] In the fields of biochemistry and molecular biology research, dextran gel purification is an important step used to separate and purify biological macromolecules such as proteins and nucleic acids.

[0003] Existing dextran gel purification equipment typically includes a base, a column, and associated supply and collection mechanisms. However, in practice, the connection process for these devices is often quite complex. The column needs to be precisely placed in a specific location within the equipment and connected to the supply and collection mechanisms via complex connectors. This connection method is not only time-consuming and labor-intensive but also prone to errors; improper connection can lead to eluent leakage or poor purification results.

[0004] Specifically, during operation, the chromatographic column needs to be precisely placed on the support frame and secured by clamps. However, existing clamps are often not designed to be flexible enough to accommodate columns of different diameters and lengths. Furthermore, the installation and removal of the clamps are cumbersome, increasing the workload of the laboratory personnel.

[0005] The supply and collection mechanisms are complex to connect: the supply mechanism typically includes multiple components such as a pump, delivery tubing, and supply connectors, and the connections between these components need to be precise and stable. However, existing connection methods are often overly complex and prone to problems during operation. Similarly, the collection mechanism also needs to be connected to the column's drain port via complex connectors, increasing the difficulty and complexity of the experiment.

[0006] To address the aforementioned issues, a purification device for dextran gel has been designed. Utility Model Content

[0007] This application provides a purification device for dextran gel to solve the problem that the connection of various components in existing dextran gel purification equipment is inconvenient in actual operation.

[0008] In a first aspect, a purification apparatus for dextran gel is provided, comprising:

[0009] The system includes a base and multiple chromatographic columns. The base is equipped with a support frame, which has multiple grooves for accommodating the chromatographic columns. Clamping devices for clamping the chromatographic columns are installed inside the grooves. Each chromatographic column has an injection port and an outlet at its upper and lower ends. A liquid supply mechanism is installed above the support frame, and a collection mechanism is installed on the base.

[0010] The liquid supply mechanism includes a liquid supply end mounted on a support frame and a liquid supply connector communicating with the liquid supply end. The liquid supply end is used to provide eluent to the liquid supply connector. One end of the liquid supply connector is sleeved on the liquid injection port of the chromatographic column to deliver the eluent into the chromatographic column.

[0011] The collection mechanism includes a liquid collection connector mounted on a base and a collection bottle arranged below the liquid collection connector. One end of the liquid collection connector is sleeved on the drain port of the chromatographic column, and the collection bottle is used to receive the eluent flowing out.

[0012] In some embodiments, multiple grooves are symmetrically arranged on the support frame, with the grooves on the upper and lower sides corresponding one-to-one.

[0013] In some embodiments, the clamping member includes two arc-shaped clamps disposed opposite each other inside the groove, the groove having two cavities opposite each other, each cavity containing a plurality of springs, and one end of each arc-shaped clamp extending into the cavity and connected to the corresponding spring.

[0014] In some embodiments, the liquid supply end includes a placement frame mounted on a support frame, a pump body mounted on the placement frame, a delivery pipe mounted on the inner side of the placement frame, and a liquid supply tank mounted on the base.

[0015] The pump body inlet is connected to the liquid supply tank, the pump body outlet is connected to the delivery pipe, and multiple liquid supply connectors are sequentially arranged on the delivery pipe.

[0016] In some embodiments, the liquid supply connector includes a valve disposed below the delivery pipe, one end of the valve being connected to a flexible tube, the bottom of the flexible tube being provided with a connector for connecting to the drain port of the chromatographic column.

[0017] In some embodiments, the liquid collection connector includes a fixing frame mounted on a base, a second valve mounted on the fixing frame, a drain pipe at the bottom of the second valve, a second flexible hose connected above the second valve, and a connector at the other end of the second flexible hose for connecting to the drain port of the chromatographic column.

[0018] This application provides a purification device for dextran gel, which simplifies the injection of eluent and the collection of samples through the design of the supply and collection mechanisms. Operators can easily connect the supply connector to the injection port of the chromatographic column and the collection connector to the discharge port, thus simplifying the operation. The device, with its precise supply and collection system, ensures that the eluent is uniformly injected into the chromatographic column and that the separated sample is accurately collected. This helps reduce experimental errors and improves the accuracy and reliability of the experiment.

[0019] The support frame and clamps ensure the stability and accuracy of the chromatographic column during the experiment, avoiding experimental failures caused by shaking or detachment.

[0020] The device's components are tightly connected and easily disassembled. This allows for convenient cleaning and maintenance after use, extending the device's lifespan. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 A three-dimensional structural illustration provided for an embodiment of this application. Figure 1 ;

[0023] Figure 2 A three-dimensional structural illustration provided for an embodiment of this application. Figure 2 ;

[0024] Figure 3 This is a three-dimensional structural diagram of the liquid collection connector provided in the embodiments of this application;

[0025] Figure 4 This is a three-dimensional structural diagram of the clamping member and support frame provided in the embodiments of this application;

[0026] Figure 5 This is a top sectional view of the support frame provided in an embodiment of this application.

[0027] In the diagram: 1. Base; 2. Support frame; 3. Chromatographic column; 4. Groove; 5. Clamping component; 51. Arc-shaped clamp; 52. Spring; 6. Liquid supply mechanism; 61. Liquid supply end; 611. Placement rack; 612. Pump body; 613. Delivery pipe; 614. Liquid supply tank; 62. Liquid supply connector; 621. Valve; 622. Hose; 623. Nozzle; 7. Collection mechanism; 71. Liquid collection connector; 711. Fixing frame; 712. Valve II; 713. Drain pipe; 714. Hose II; 715. Nozzle; 72. Collection bottle. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0029] This application provides a purification device for dextran gel, which solves the problem that the connection of various components in existing dextran gel purification equipment is inconvenient in actual operation.

[0030] Please see Figures 1-5 A purification device for dextran gel includes: a base 1 and multiple chromatographic columns 3. A support frame 2 is provided on the base 1. The support frame 2 is provided with multiple grooves 4 for accommodating the chromatographic columns 3. Clamping elements 5 for clamping the chromatographic columns 3 are provided inside the grooves 4. The chromatographic columns 3 have injection ports and drainage ports at their upper and lower ends. A liquid supply mechanism 6 is provided above the support frame 2, and a collection mechanism 7 is provided on the base 1.

[0031] The liquid supply mechanism 6 includes a liquid supply end 61 disposed on the support frame 2 and a liquid supply connector 62 communicating with the liquid supply end 61. The liquid supply end 61 is used to provide eluent to the liquid supply connector 62. One end of the liquid supply connector 62 is sleeved on the liquid injection port of the chromatographic column 3 for sending the eluent into the chromatographic column 3.

[0032] The collection mechanism 7 includes a liquid collection connector 71 disposed on the base 1 and a collection bottle 72 disposed below the liquid collection connector 71. One end of the liquid collection connector 71 is sleeved on the drain port of the chromatographic column 3, and the collection bottle 72 is used to receive the eluent flowing out.

[0033] Multiple chromatographic columns 3 are placed in the grooves 4 on the support frame 2, and the columns 3 are clamped and fixed using the clamping parts 5 inside the grooves 4. This ensures the stability and accuracy of the chromatographic columns 3 during the experiment.

[0034] The liquid supply mechanism 6 is located above the support frame 2, and its liquid supply end 61 is connected to the eluent source via a pipe. When the pump of the liquid supply end 61 is started, the eluent is pumped into the liquid supply connector 62. One end of the liquid supply connector 62 is fitted onto the injection port of the chromatographic column 3, thereby delivering the eluent into the chromatographic column 3. The eluent interacts with the dextran gel inside the chromatographic column 3, allowing different components in the sample to be separated according to their adsorption capacity on the gel.

[0035] The eluent after separation by column 3 flows out from the outlet of column 3 and enters the collection mechanism 7 through the collection connector 71 attached to the outlet. The collection connector 71 guides the eluent to the collection bottle 72 arranged below it, thereby realizing the collection of the separated sample.

[0036] The design of the support frame 2 and the clamping component 5 ensures the stability and accuracy of the chromatographic column 3 during the experiment, avoiding experimental failures caused by shaking or falling off.

[0037] The design of the supply mechanism 6 and the collection mechanism 7 makes the injection of eluent and the collection of samples more convenient. Operators can easily connect the supply connector 62 to the injection port of the column 3 and the collection connector 71 to the discharge port, thus simplifying the operation. This device, through its precise supply and collection system, ensures that the eluent is uniformly injected into the column 3 and that the separated sample is accurately collected. This helps reduce experimental errors and improves the accuracy and reliability of the experiment.

[0038] The device's components are tightly connected and easily disassembled. This allows for convenient cleaning and maintenance after use, extending the device's lifespan.

[0039] This device can accommodate multiple chromatographic columns 3, and different columns can be selected for separation and purification according to experimental needs. Meanwhile, the design of the liquid supply mechanism 6 and the collection mechanism 7 is flexible, accommodating different concentrations of eluent and different collection methods. This allows the device to meet a variety of experimental requirements and has broad application prospects.

[0040] It should be noted that in this embodiment, the multiple grooves 4 are symmetrically arranged on the support frame 2, and the grooves 4 on the upper and lower sides correspond one-to-one.

[0041] The symmetrical grooves 4 make the weight distribution of the support frame 2 more uniform when it supports multiple chromatographic columns 3, avoiding excessive local stress on the support frame due to weight concentration, thereby enhancing the structural stability of the entire device.

[0042] Due to the symmetry of the groove 4, when the chromatographic column 3 is clamped in the groove 4, it can be more firmly fixed on the support frame 2, reducing the shaking caused by external forces such as liquid flow during the experiment, thus ensuring the accuracy and reliability of the experiment.

[0043] The symmetrical grooves 4 make the installation and removal of the chromatographic column 3 more convenient. Operators can easily place the column into the corresponding groove from above or below and secure it using the clamps 5, without worrying about operational difficulties caused by improper positioning.

[0044] In one embodiment, the clamping member 5 includes two arc-shaped clamping plates 51 disposed opposite to each other inside the groove 4. Two cavities are opened opposite to each other inside the groove 4. A plurality of springs 52 are disposed inside the cavity 51. One end of the arc-shaped clamping plate 51 extends into the cavity and is connected to the corresponding spring 52.

[0045] The shape of the arc-shaped clamp 51 matches the outer wall of the chromatographic column 3, ensuring that the clamp 5 can fit tightly against the chromatographic column 3, thereby providing stable support and fixation.

[0046] The groove 4 has two opposing cavities, each containing a number of springs 52. One end of the arc-shaped clamp 51 extends into the cavity and connects to the corresponding spring 52, giving the arc-shaped clamp 51 a certain degree of elasticity. This allows it to adaptively adjust according to the diameter of the chromatographic column 3, ensuring both stability and flexibility in clamping.

[0047] With the combined use of the arc-shaped clamp 51 and the spring 52, the clamp 5 can fit tightly against the chromatographic column 3, effectively preventing the chromatographic column from shaking or falling off during the experiment, thereby enhancing the stability of the entire device.

[0048] Because the arc-shaped clamp 51 has a certain degree of elasticity and the spring 52 provides a certain degree of cushioning, the clamping member 5 can accommodate chromatographic columns 3 of different diameters. This design makes the purification device suitable for chromatographic columns of various specifications, improving its practicality and flexibility.

[0049] In one embodiment, the liquid supply end 61 includes a placement frame 611 mounted on a support frame 2, a pump body 612 mounted on the placement frame 611, a delivery pipe 613 mounted on the inner side of the placement frame 611, and a liquid supply tank 614 mounted on the base 1; the inlet of the pump body 612 is connected to the liquid supply tank 614, the outlet of the pump body 612 is connected to the delivery pipe 613, and a plurality of liquid supply connectors 62 are sequentially mounted on the delivery pipe 613.

[0050] The mounting bracket 611 is securely mounted on the support frame 2, providing a stable support platform for the pump body 612.

[0051] The pump body 612 is the core component of the liquid supply system, responsible for extracting the eluent from the liquid supply tank 614 and delivering it to each liquid supply connector 62 through the delivery pipe 613.

[0052] The delivery pipe 613 is connected between the outlet of the pump body 612 and multiple liquid supply connectors 62, and is responsible for delivering the eluent from the pump body 612 to the injection port of each chromatographic column 3.

[0053] The supply tank 614 is mounted on the base 1 and is used to store the eluent.

[0054] In actual operation, the experimenter only needs to start the pump body 612 to deliver the eluent from the supply tank 614 to the delivery tube 613, and then send it into the injection port of each chromatographic column 3 through the delivery tube 613, without the need for manual operation or adjustment.

[0055] In one embodiment, the liquid supply connector 62 includes a valve 621 disposed below the delivery pipe 613, the bottom end of the valve 621 is connected to a hose 622, the bottom of the hose 622 is provided with a nozzle 623, the nozzle 623 is used to connect to the drain port of the chromatographic column 3.

[0056] Valve 621 is a key component of the liquid supply connector 62, responsible for controlling the flow of eluent. When it is necessary to supply eluent to the column 3, valve 621 is opened, and the eluent flows into the column through hose 622 and nozzle 623; when it is not necessary to supply eluent, valve 621 is closed to cut off the flow of eluent.

[0057] The hose 622, as the connecting component between the delivery tube 613 and the nozzle 623, has a certain degree of flexibility and elasticity, which allows the hose 622 to adapt to the chromatographic column 3 at different positions and angles, ensuring that the eluent can be smoothly delivered to the chromatographic column.

[0058] The connector 623 is the connecting component between the liquid supply connector 62 and the liquid outlet of the chromatographic column 3, ensuring a tight fit with the liquid outlet of the chromatographic column 3 to prevent leakage of the eluent.

[0059] The addition of valve 621 allows researchers to precisely control the flow of the eluent, preventing leakage or waste of the eluent due to misoperation or equipment failure.

[0060] The components of the liquid supply connector 62 are tightly connected and easy to disassemble. When cleaning or replacing components is required, the experimenter can easily disconnect and operate, reducing maintenance difficulty and cost.

[0061] In one embodiment, the liquid collection connector 71 includes a fixing frame 711 mounted on the base 1, a second valve 712 mounted on the fixing frame 711, a drain pipe 713 mounted at the bottom of the second valve 712, a second flexible hose 714 connected above the second valve 712, and a connector 715 connected at the other end of the second flexible hose 714. The connector 715 is used to connect to the drain port of the chromatographic column 3.

[0062] The mounting bracket 711 provides a solid support for valve 712.

[0063] Valve 2 712 is a component that controls the drainage process. By opening or closing valve 2 712, the experimenter can flexibly control the flow of liquid drained from column 3.

[0064] The drain pipe 713 is connected to the bottom of valve 2 712 and is responsible for guiding the liquid discharged from the chromatographic column 3 to the designated collection container.

[0065] The flexible hose 714, as the connecting component between valve 712 and connector 715, has a certain degree of flexibility and elasticity, which allows the flexible hose 714 to adapt to the chromatographic column 3 at different positions and angles, ensuring that the drainage process can proceed smoothly.

[0066] The connector 715 is the connecting component between the liquid collection connector 71 and the liquid outlet of the chromatographic column 3, ensuring a tight connection with the liquid outlet of the chromatographic column and preventing liquid leakage.

[0067] The addition of valve 2 712 allows researchers to flexibly control the drainage process. Valve 2 712 can be opened or closed at any time as needed to adjust the drainage rate and volume.

[0068] The components of the liquid collection connector 71 are tightly connected and easily disassembled. When cleaning or replacing components is required, the experimenter can easily disconnect the connection and perform the operation, reducing maintenance difficulty and cost.

[0069] In the description of this application, it should be noted that the terms "upper," "lower," etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. Unless otherwise expressly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two elements. For those skilled in the art, the specific meaning of the above terms in this application can be understood according to the specific circumstances.

[0070] It should be noted that in this application, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0071] The above description is merely a specific embodiment of this application, enabling those skilled in the art to understand or implement this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A purification device for dextran gel, characterized in that, include: The base (1) and multiple chromatographic columns (3) are provided. A support frame (2) is provided on the base (1). The support frame (2) is provided with multiple grooves (4) for accommodating the chromatographic columns (3). Clamping parts (5) for clamping the chromatographic columns (3) are provided inside the grooves (4). The chromatographic columns (3) have injection ports and drainage ports at the upper and lower ends. A liquid supply mechanism (6) is provided above the support frame (2). A collection mechanism (7) is provided on the base (1). The liquid supply mechanism (6) includes a liquid supply end (61) disposed on the support frame (2) and a liquid supply connector (62) communicating with the liquid supply end (61). The liquid supply end (61) is used to provide eluent to the liquid supply connector (62). One end of the liquid supply connector (62) is sleeved on the liquid injection port of the chromatographic column (3) for sending the eluent into the chromatographic column (3). The collection mechanism (7) includes a liquid collection connector (71) disposed on the base (1) and a collection bottle (72) disposed below the liquid collection connector (71). One end of the liquid collection connector (71) is sleeved on the drain port of the chromatographic column (3), and the collection bottle (72) is used to receive the eluent flowing out.

2. The purification apparatus for dextran gel as described in claim 1, characterized in that: Multiple grooves (4) are symmetrically arranged on the support frame (2), with the grooves (4) on the upper and lower sides corresponding one-to-one.

3. The purification apparatus for dextran gel as described in claim 1, characterized in that: The clamping member (5) includes two arc-shaped clamping plates (51) arranged opposite to each other inside the groove (4). Two cavities are opened opposite to each other inside the groove (4). Several springs (52) are arranged inside the cavities (51). One end of the arc-shaped clamping plate (51) extends into the cavity and is connected to the corresponding spring (52).

4. The purification apparatus for dextran gel as described in claim 1, characterized in that: The liquid supply end (61) includes a placement frame (611) set on the support frame (2), a pump body (612) is set on the placement frame (611), a delivery pipe (613) is set on the inner side of the placement frame (611), and a liquid supply tank (614) is set on the base (1). The pump body (612) inlet is connected to the liquid supply tank (614), the pump body (612) outlet is connected to the delivery pipe (613), and a plurality of liquid supply connectors (62) are sequentially arranged on the delivery pipe (613).

5. The purification apparatus for dextran gel as described in claim 4, characterized in that: The liquid supply connector (62) includes a valve (621) located below the delivery pipe (613). The bottom end of the valve (621) is connected to a flexible hose (622). The bottom of the flexible hose (622) is provided with a nozzle (623), which is used to connect to the drain port of the chromatographic column (3).

6. The purification apparatus for dextran gel as described in claim 1, characterized in that: The liquid collection connector (71) includes a fixing frame (711) set on the base (1), a second valve (712) is set on the fixing frame (711), a drain pipe (713) is set at the bottom of the second valve (712), a second hose (714) is connected above the second valve (712), and a connector (715) is connected at the other end of the second hose (714). The connector (715) is used to connect to the drain port of the chromatographic column (3).

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