Novel device for researching plant-fungus-bacterium interaction

Abstract

The utility model discloses a novel device for studying plant-fungus-bacteria three-boundary interaction, which comprises a culture box, a bottom plate and a sampling piece, the culture box is fixedly connected to the upper surface of the bottom plate, a planting cavity, a fungus cavity and a bacteria cavity are arranged in the culture box, and the sampling piece is arranged in the bottom plate. A top cover for sealing the planting cavity, the fungus cavity and the bacteria cavity is arranged at the top of the incubator. According to the novel device for researching the plant-fungus-bacterium three-boundary interaction, an electric telescopic rod is started to drive a sampling rod to move downwards, so that the sampling rod penetrates through a through hole to penetrate into a planting cavity, a fungus cavity and a bacterium cavity to sample soil, and when a closed cylinder is in contact with a soil layer, the closed cylinder slides upwards automatically, so that the sampling rod is driven to move downwards; when the sampling rod moves upwards, the sealing cylinder moves downwards, and the bottom end of the sampling rod is sealed through the gravity of the sealing cylinder under the cooperation of the sealing cylinder and a baffle ring, so that the contact between a sampling part and external air is reduced, and pollution is avoided.

Description

Technical Field

[0001] This utility model relates to the field of biological research experimental equipment technology, specifically a novel device for studying the interactions among the plant-fungus-bacteria tri-kingdom. Background Technology

[0002] In modern biology and ecology, in-depth research into the interactions among plants, fungi, and bacteria is crucial for understanding ecosystem function, maintaining biodiversity, and promoting sustainable agricultural development. As research progresses, the need for accurate and comprehensive data becomes increasingly urgent, making simultaneous sampling and testing of three soil species often necessary.

[0003] However, due to the spatial heterogeneity of the distribution of plants, fungi, and bacteria in soil, and the differences in their respective ecological niches and functions, it is difficult to fully reveal the complex interaction mechanisms between the three kingdoms by testing a single soil sample. Therefore, it is necessary to sample the soil containing plants, fungi, and bacteria at irregular intervals for research. However, existing sampling equipment cannot sample soil quickly and effectively, and is prone to contaminating the samples. Therefore, further optimization is needed to address these issues. Utility Model Content

[0004] In view of the shortcomings of the prior art, this utility model provides a novel device for studying the interaction of the plant-fungus-bacteria tri-kingdom, which solves the problems mentioned in the background art above.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution: a novel device for studying the interactions among the plant-fungus-bacteria kingdoms, comprising an incubator, a base plate, and a sampling component;

[0006] The incubator is fixedly connected to the upper surface of the base plate. The interior of the incubator is provided with an implantation chamber, a fungal chamber, and a bacterial chamber. The top of the incubator is provided with a top cover that seals the implantation chamber, the fungal chamber, and the bacterial chamber. The inner side wall of the implantation chamber is provided with a fixing hole that communicates with the fungal chamber and the bacterial chamber.

[0007] The sampling component is located on the upper surface of the top cover. A through hole for use with the sampling component is provided on the upper surface of the top cover. The sampling component includes an auxiliary cylinder fixedly connected to the upper surface of the top cover. An electric telescopic rod is fixedly connected to the top end of the auxiliary cylinder. The output end of the electric telescopic rod extends into the interior of the auxiliary cylinder and is threadedly connected to a sampling rod. A closed cylinder is fitted on the outer wall of the bottom end of the sampling rod. A retaining ring for limiting the closed cylinder is provided on the outer wall of the sampling rod.

[0008] This utility model has the following beneficial effects:

[0009] This is a novel device for studying the interactions between the plant, fungi, and bacteria kingdoms. Activating the electric telescopic rod moves the sampling rod downwards, allowing it to pass through the through-holes and penetrate deep into the planting chamber, fungal chamber, and bacterial chamber to sample the soil. When the sealing cylinder contacts the soil layer, it automatically slides upwards without affecting the sampling rod's sampling. As the sampling rod moves upwards, the sealing cylinder moves downwards and, through its own gravity and in conjunction with the retaining ring, seals the bottom of the sampling rod, reducing contact between the sampling area and the outside air and preventing contamination.

[0010] This is a novel device for studying the interactions between the plant, fungi, and bacteria kingdoms; the top of the sampling rod is threadedly connected to the output end of the electric telescopic rod, which allows for easy disassembly of the sampling rod and facilitates the detection and research of samples collected at the bottom of the sampling rod.

[0011] This is a novel device for studying the interactions between the plant, fungi, and bacteria kingdoms. The start-up drive block can drive the drive ring to rotate, which, in conjunction with gear one, gear two, rack one, and rack two, synchronously drives cover plate one, cover plate two, and cover plate three to rotate, facilitating the adjustment of the sampling position as needed and improving the flexibility of sampling. Attached Figure Description

[0012] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0013] Figure 2 This is a cross-sectional view of the incubator of this utility model;

[0014] Figure 3 This utility model Figure 2 Enlarged structural diagram at point A in the middle;

[0015] Figure 4 This utility model Figure 2 Enlarged structural diagram at point B;

[0016] Figure 5 This utility model Figure 2 Enlarged structural diagram at point C.

[0017] The components are as follows: 1. Incubator; 2. Base plate; 3. Planting chamber; 4. Fungal chamber; 5. Bacterial chamber; 6. Top cover; 601. Cover plate one; 602. Cover plate two; 603. Cover plate three; 7. Auxiliary cylinder; 8. Electric telescopic rod; 9. Sampling rod; 10. Fixing hole; 11. Sealing cylinder; 12. Retaining ring; 13. Through hole; 14. Sampling slot; 15. Support column; 16. Baffle; 17. Toothed layer; 18. Gear one; 19. Gear two; 20. Gear rack one; 21. Gear rack two; 22. Drive block; 23. Drive wheel. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1 to 5 This utility model provides a novel device for studying the interaction between the plant-fungus-bacteria kingdom; it includes an incubator 1, a base plate 2, and a sampling device; an anti-slip layer is provided on the bottom of the base plate 2 to reduce slippage and maintain stability when the device is placed on a table.

[0020] The incubator 1 is fixedly connected to the upper surface of the base plate 2. The incubator 1 has an inoculation chamber 3, a fungal chamber 4 and a bacterial chamber 5 inside. The top of the incubator 1 is provided with a top cover 6 that seals the inoculation chamber 3, the fungal chamber 4 and the bacterial chamber 5. The inner side wall of the inoculation chamber 3 has a fixed hole 10 that communicates with the fungal chamber 4 and the bacterial chamber 5.

[0021] like Figure 1 and Figure 2 As shown, the planting chamber 3, fungal chamber 4 and bacterial chamber 5 are filled with soil suitable for the growth of plants, fungi and bacteria. Several fixing holes 10 are provided at the connection points with the fungal chamber 4 and bacterial chamber 5. The incubator 1 is made of transparent material, which makes it easy to observe the plant roots and stems inside the planting chamber 3 passing through the fixing holes 10 and reacting with the fungal chamber 4 and bacterial chamber 5, so as to facilitate research.

[0022] The sampling component is located on the upper surface of the top cover 6. A through hole 13 is provided on the upper surface of the top cover 6 to cooperate with the sampling component. The sampling component includes an auxiliary cylinder 7 fixedly connected to the upper surface of the top cover 6. An electric telescopic rod 8 is fixedly connected to the top end of the auxiliary cylinder 7. The output end of the electric telescopic rod 8 extends into the interior of the auxiliary cylinder 7 and is threadedly connected to a sampling rod 9. A closed cylinder 11 is sleeved on the outer wall of the bottom end of the sampling rod 9. A retaining ring 12 for limiting the closed cylinder 11 is provided on the outer wall of the sampling rod 9.

[0023] like Figure 1 and Figure 2 As shown, the bottom of the auxiliary cylinder 7 is hollow, and the top of the sampling rod 9 is provided with a threaded post (shown in the figure, not labeled). The bottom of the electric telescopic rod 8 is provided with a threaded groove that matches the threaded post, so that the sampling rod 9 can be raised to the hollow part for disassembly, and the soil sample at the bottom of the sampling rod 9 can be taken out for testing and research.

[0024] The outer wall of the bottom end of the sampling rod 9 is provided with a sampling groove 14, which is used in conjunction with the closed cylinder 11. The upper surface of the bottom end of the auxiliary cylinder 7 is rotatably connected to a support column 15. The bottom end of the support column 15 passes through the top cover 6 and is fixedly connected to a baffle 16, which is used in conjunction with the through hole 13. There are three sampling grooves 14, which are equidistantly arranged on the outer wall of the bottom end of the sampling rod 9.

[0025] Multiple sampling slots 14 can collect soil samples from different soil depths for comprehensive observation. The rotating support column 15 can drive the baffle 16 at its top to rotate. The baffle 16 is circular and larger than the through hole 13. The purpose of this design is to rotate to the through hole 13 when not sampling, thereby reducing the amount of outside air entering the interior and preventing contamination of the soil inside.

[0026] The top cover 6 includes a cover plate 601 on top of the implantation cavity 3, a cover plate 602 on top of the fungal cavity 4, and a cover plate 603 on top of the bacterial cavity 5; the side walls of the cover plate 601, the cover plate 602 and the cover plate 603 are provided with a toothed layer 17.

[0027] The top of the incubator 1 is provided with a side gear 18 and a gear 29 that mesh with the toothed layer 17. The incubator 1 is provided with a toothed bar 10 and a toothed bar 21 that mesh with the gear 18 and the gear 29. The bottom ends of the toothed bar 10 and the toothed bar 21 mesh with the cover plate 2 602 and the cover plate 3 603.

[0028] Inside the incubator 1, near the cover plate 603, there is a drive block 22. The output end of the drive block 22 is fixedly connected to a drive wheel 23 and meshes with the cover plate 603.

[0029] When the drive block 22 is started (powered by a motor), it can drive the cover plate 3 603 to rotate. The cover plate 3 603 drives the rack 21 to rotate. The rack 21 drives the gear 2 19 to rotate. The gear 2 19 drives the cover plate 1 601 to rotate. The cover plate 1 601 drives the gear 1 18 to rotate. The gear 1 18 drives the rack 20 to rotate. The rack 20 drives 602 to rotate.

[0030] When cover plate 1 601, cover plate 2 602 and cover plate 3 603 rotate, they can drive the auxiliary cylinder 7 to rotate, which makes it convenient to adjust the sampling component to take samples at different positions and improves the flexibility of sampling.

[0031] All electrical components mentioned in this article are connected to an external main controller and 220V AC mains power, and the main controller can be a conventional known device such as a computer that can control it.

[0032] In this invention, the working steps of the device are as follows:

[0033] In use, soil is placed inside the planting chamber 3, fungal chamber 4, and bacterial chamber 5, and plants, fungi, and bacteria are placed there. When sampling is required, the soil inside is sampled through the sampling device. When the sampling position needs to be adjusted, the top cover 6 is rotated by the drive block 22, and the sampling device is used to take the sample.

[0034] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A novel device for studying the interactions among the plant-fungus-bacteria kingdoms, characterized in that: Includes an incubator (1), a base plate (2), and a sampling piece; The incubator (1) is fixedly connected to the upper surface of the base plate (2). The incubator (1) is provided with an inoculation chamber (3), a fungal chamber (4) and a bacterial chamber (5). The top of the incubator (1) is provided with a top cover (6) that closes the inoculation chamber (3), the fungal chamber (4) and the bacterial chamber (5). The inner side wall of the inoculation chamber (3) is provided with a fixed hole (10) that communicates with the fungal chamber (4) and the bacterial chamber (5). The sampling component is located on the upper surface of the top cover (6). A through hole (13) for use with the sampling component is provided on the upper surface of the top cover (6). The sampling component includes an auxiliary cylinder (7) fixedly connected to the upper surface of the top cover (6). An electric telescopic rod (8) is fixedly connected to the top end of the auxiliary cylinder (7). The output end of the electric telescopic rod (8) extends into the interior of the auxiliary cylinder (7) and is threadedly connected to a sampling rod (9). A closed cylinder (11) is sleeved on the outer wall of the bottom end of the sampling rod (9). A retaining ring (12) for limiting the closed cylinder (11) is provided on the outer wall of the sampling rod (9).

2. The novel device for studying the interactions among the plant-fungus-bacteria kingdoms according to claim 1, characterized in that: The sampling rod (9) has a sampling groove (14) on the outer wall at the bottom end and is used in conjunction with the closed cylinder (11). The upper surface of the bottom end of the auxiliary cylinder (7) is rotatably connected to a support column (15). The bottom end of the support column (15) passes through the top cover (6) and is fixedly connected to a baffle (16) and is used in conjunction with a through hole (13).

3. A novel device for studying the interactions among the plant-fungus-bacteria kingdoms according to claim 2, characterized in that: The top cover (6) includes a cover plate one (601) on the top of the implantation cavity (3), a cover plate two (602) on the top of the fungal cavity (4), and a cover plate three (603) on the top of the bacterial cavity (5). The sidewalls of the cover plate one (601), cover plate two (602) and cover plate three (603) are provided with a toothed layer (17).

4. A novel device for studying the interactions among the plant-fungus-bacteria kingdoms according to claim 3, characterized in that: The top of the incubator (1) is provided with a side gear one (18) and a gear two (19) that mesh with the toothed layer (17).

5. A novel device for studying the interactions among the plant-fungus-bacteria kingdoms according to claim 4, characterized in that: The incubator (1) is equipped with a gear rod (20) and a gear rod (21) that mesh with gear one (18) and gear two (19). The bottom ends of the gear rod one (20) and gear rod two (21) are meshed with cover plate two (602) and cover plate three (603).

6. A novel device for studying the interactions among the plant-fungus-bacteria kingdoms according to claim 5, characterized in that: Inside the incubator (1), near the cover plate (603), there is a drive block (22). The output end of the drive block (22) is fixedly connected to a drive wheel (23) and meshes with the cover plate (603).

7. A novel device for studying the interactions among the plant-fungus-bacteria kingdoms according to claim 2, characterized in that: The number of sampling slots (14) is three, and they are equidistantly located on the outer wall of the bottom end of the sampling rod (9).

Images