Protein immunochromatography detection kit

Abstract

The utility model discloses a protein immunochromatography detection kit, which belongs to the technical field of biochips and diagnostic reagents, and comprises a chromatography strip and a bottom plate, the chromatography strip is placed on the bottom plate, the chromatography strip comprises a sample pad, a first combination pad, a nitrocellulose membrane and a water absorption pad, clamping grooves are oppositely arranged on the bottom plate, and the first combination pad and the second combination pad are oppositely arranged on the bottom plate. When the chromatography strip is placed on the bottom plate, the clamping grooves are located in the two sides of the nitrocellulose membrane, a flow guide sleeve is arranged at the upper end of the nitrocellulose membrane, and the flow guide sleeve is clamped in the clamping grooves and tightly presses the nitrocellulose membrane. According to the protein immunochromatography detection kit disclosed by the utility model, a sample flows more uniformly, and a detection result is more accurate.

Description

Technical Field

[0001] This utility model relates to the field of biochip and diagnostic reagent technology, and in particular to a protein immunochromatographic detection kit. Background Technology

[0002] Fluorescent immunochromatography is an analytical technique based on antibody-antigen specific recognition. It utilizes the highly specific binding between antigens and antibodies to detect and quantify target molecules. For large molecular antigens (such as proteins and viruses) with multiple antigenic determinants, a "sandwich" type double-antibody immunochromatographic method is usually used. The analyte first binds to the fluorescently labeled antibody under the action of the mobile phase, and then binds to the coating antibody when it reaches the detection line, forming a double-antibody sandwich structure.

[0003] Existing fluorescent immunochromatographic assay kits generally consist of a base plate and a sample pad, a first conjugation pad, a second conjugation pad, a nitrocellulose membrane, and an absorbent pad. For example, Chinese Patent CN 118275698 B discloses a cardiac troponin I immunochromatographic assay card, kit, and its application. The cardiac troponin I immunochromatographic assay card includes a test strip, which consists of a rigid base plate and a sample pad, a first conjugation pad, a second conjugation pad, a nitrocellulose membrane, and an absorbent pad sequentially adhered to the rigid base plate. The first conjugation pad is conjugated with biotin-labeled cTnI antibody 1# and biotin-labeled cTnI antibody 2#. The second conjugation pad is conjugated with AIE fluorescent microsphere-labeled cTnI antibody 3# and AIE fluorescent microsphere-labeled dinitrophenol bovine serum albumin conjugate.

[0004] In actual use, the above-mentioned kit may experience problems such as uneven flow of the sample on the nitrocellulose membrane and displacement of the overlapping parts of the first conjugate pad, the second conjugate pad, and the nitrocellulose membrane. Utility Model Content

[0005] The technical problem to be solved by this invention is to design a novel protein immunochromatographic assay kit. This kit first solves the problem of sample flow uniformity, and secondly solves the problem of displacement at the overlapping points of the first conjugate pad, the second conjugate pad, and the nitrocellulose membrane.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a protein immunochromatographic assay kit, comprising a chromatography strip and a base plate, wherein the chromatography strip is placed on the base plate, and the chromatography strip includes a sample pad, a first binding pad, a nitrocellulose membrane, and an absorbent pad. The base plate has slots arranged opposite each other. When the chromatography strip is placed on the base plate, the slots are located on both sides of the nitrocellulose membrane. A flow guide sleeve is provided at the upper end of the nitrocellulose membrane, the flow guide sleeve engaging in the slots and pressing the nitrocellulose membrane. The flow guide sleeve has several grids that divide the nitrocellulose membrane into several channels.

[0007] Furthermore, an observation window is provided at the top of the flow guide sleeve.

[0008] Furthermore, a connecting sleeve is provided at the connection between the first binding pad and the sample pad and the nitrocellulose membrane, and the connecting sleeve is fitted at the connection to fix the connection.

[0009] Furthermore, a fixing groove is provided on the base plate, which is used to fix the connecting sleeve.

[0010] Furthermore, the base plate is provided with a plurality of protrusions, which are used to support the microarray chromatography strips.

[0011] Furthermore, fixing platforms are respectively provided at both ends and on the sides of the base plate, and the fixing platforms are used to fix the chromatography strips.

[0012] Beneficial effects: This application uses a flow guide sleeve to press and fix the nitrocellulose membrane onto a base plate. Multiple grids are set within the flow guide sleeve, which press the nitrocellulose membrane into several relatively independent channels. Different antibodies can be pre-embedded in each channel. When the sample flows onto the nitrocellulose membrane, the pressure from the grids forces the sample to flow only within its respective channel, resulting in more uniform sample flow and a more stable detection signal.

[0013] Secondly, this application solves the problem of displacement at the overlapping point of the first binding pad, the second binding pad, and the nitrocellulose membrane by setting a connecting sleeve at the connection point between the first binding pad, the sample pad, and the nitrocellulose membrane to fix the connection point.

[0014] Finally, this application uses protrusions to lift the chromatography strips away from the base plate, preventing the sample from sticking to the base plate during flow, thus avoiding sample waste and uneven flow. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of the chromatography detection kit of this utility model.

[0016] Figure 2 This is a schematic diagram of the structure of the flow guide sleeve of this utility model.

[0017] Figure 3 This is a schematic diagram of the connecting sleeve of this utility model.

[0018] Figure 4 This is a schematic diagram of another embodiment of the chromatography detection kit of this utility model.

[0019] Figure 5 This is a structural schematic diagram of the boss and the fixed platform of this utility model.

[0020] Wherein 1-sample pad, 2-first binding pad, 3-nitrocellulose membrane, 4-absorbent pad, 5-guide sleeve, 51-grid, 52-channel, 53-observation window, 6-base plate, 7-slot, 8-connecting sleeve, 9-fixing groove, 10-protrusion, 11-fixing platform. Detailed Implementation

[0021] To enhance understanding of this utility model, it will be described in detail below with reference to the embodiments and accompanying drawings. These embodiments are only used to explain this utility model and do not limit the scope of protection of this utility model. Example 1

[0022] like Figure 1 The image shows a protein immunochromatographic assay kit, which includes a chromatography strip and a base plate 6. The chromatography strip is placed on the base plate 6. The chromatography strip includes a sample pad 1, a first binding pad 2, a nitrocellulose membrane 3, and an absorbent pad 4. The sample is dropped onto the sample pad 1. Fluorescent or biotin-labeled antibodies are embedded on the first binding pad 2, and specific antibodies are embedded on the nitrocellulose membrane.

[0023] The base plate 6 has slots 7 arranged opposite each other. When the chromatography strip is placed on the base plate 6, the slots 7 are located on both sides of the nitrocellulose membrane 3. A flow guide sleeve 5 is provided at the upper end of the nitrocellulose membrane 3. The two ends of the flow guide sleeve 5 are engaged in the slots 7 and press the nitrocellulose membrane 3 tightly.

[0024] like Figure 1 As shown, the slot 7 is a rectangular through hole, and the two ends of the guide sleeve 5 are bent. During installation, the two ends of the guide sleeve 5 can be pressed firmly into the slot 7. Other fixing methods are also acceptable, as long as the guide sleeve 5 is fixed to the base plate.

[0025] like Figure 2 As shown, the flow guide sleeve 5 is provided with several grids 51. After the flow guide sleeve 5 is installed, the grids 51 press against the nitrocellulose membrane 3 and divide the nitrocellulose membrane 3 into several channels 52. Therefore, the embedding position of the specific antibody can be set in advance according to the position of the channel 52, and the antibody can be embedded in the channel 52.

[0026] An observation window 53 is provided at the top of the flow guide sleeve 5 to facilitate the observation of the test results.

[0027] During testing, after the sample to be tested is added to the sample pad, it first flows to the first binding pad 2, binds to the antibody labeled on the first binding pad 2, and then flows to the nitrocellulose membrane 3. Due to the pressure applied to the nitrocellulose membrane 3 by the grid 51 of the flow guide sleeve 5, the sample can only flow in the channel 52 on the nitrocellulose membrane 3. Example 2

[0028] like Figure 3 and Figure 4 As shown, the difference between this embodiment and embodiment 1 is that a connecting sleeve 8 is provided at the connection between the first bonding pad 2 and the sample pad 1 and the nitrocellulose membrane 3 to fix the two connection points.

[0029] The specific structure of the connecting sleeve 8 is as follows: Figure 3 As shown, it is a hollow shell structure, the length of which must cover the distance between the two connection points, and the connecting sleeve 8 is fitted at the two connection points to fix it.

[0030] Meanwhile, a fixing groove 9 is provided on the base plate 6 to fix the connecting sleeve 8. The structure of the fixing groove 9 is as follows: Figure 4 As shown, the connecting sleeve 8 can be engaged in the fixing groove 9. Example 3

[0031] like Figure 4 As shown, a number of protrusions 10 are provided on the base plate 6 to support the chromatography strips. Fixing platforms 11 are respectively provided at both ends and on the sides of the base plate 6 to fix the chromatography strips.

[0032] like Figure 5 The diagram shows the structure of the boss 10 and the fixed platform 11 combined together. If there is enough space in the base plate, the boss 10 and the fixed platform 11 can be set separately or in other styles, as long as the chromatography strip is supported and fixed.

[0033] The embodiments disclosed herein are preferred embodiments, but are not limited thereto. Those skilled in the art can readily grasp the spirit of this utility model based on the above embodiments and make different extensions and variations. However, as long as they do not depart from the spirit of this utility model, they are all within the protection scope of this utility model.

Claims

1. A protein immunochromatographic test kit comprising a chromatographic strip and a base plate (6), the chromatographic strip being placed on the base plate (6), the chromatographic strip comprising a sample pad (1), a first binding pad (2), a nitrocellulose membrane (3), and a water absorbing pad (4), characterized in that, The bottom plate (6) is oppositely provided with clamping grooves (7), when the chromatographic strip is placed on the bottom plate (6), the clamping grooves (7) are located at both sides of the nitrocellulose membrane (3), the upper end of the nitrocellulose membrane (3) is provided with a flow guide sleeve (5), the flow guide sleeve (5) is clamped in the clamping groove (7) and presses the nitrocellulose membrane (3), a plurality of grid bars (51) are arranged in the flow guide sleeve (5), and the grid bars (51) separate the nitrocellulose membrane (3) into a plurality of channels (52).

2. The protein immunochromatographic test kit according to claim 1, characterized by, The top end of the flow guide sleeve (5) is provided with an observation window (53).

3. The protein immunochromatographic test kit according to claim 1, characterized by, The connecting part of the first combination pad (2) and the sample pad (1) and the nitrocellulose membrane (3) is provided with a connecting sleeve (8), and the connecting sleeve (8) is sleeved on the connecting part for fixing the connecting part.

4. The protein immunochromatographic test kit according to claim 3, characterized by, The bottom plate (6) is oppositely provided with fixed grooves (9), and the fixed grooves (9) are used for fixing the connecting sleeve (8).

5. The protein immunochromatographic test kit according to claim 1, characterized by, A plurality of convex bosses (10) are arranged on the bottom plate (6), and the convex bosses (10) are used for supporting the microarray chromatographic strip.

6. The protein immunochromatographic test kit according to claim 1, characterized by, The two ends and the side of the bottom plate (6) are oppositely provided with fixed tables (11), and the fixed tables (11) are used for fixing the chromatographic strip.

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