Special culture dish for embryo freezing

Abstract

The culture dish comprises a culture dish body, a plurality of first storage holes are formed in the left side of the upper end of the culture dish body, a plurality of second storage holes are formed in the right side of the upper end of the culture dish body, the diameter of each first storage hole is larger than that of each second storage hole, and a containing groove is formed in the rear side of the upper end of the culture dish body. A first clamping groove extending to the outer side of the culture dish is formed in the left end of the placement groove, a second clamping groove extending to the outer side of the culture dish is formed in the right end of the placement groove, and the first clamping groove and the second clamping groove are parallel front and back. During use, after the freezing carrying rod is opened, the freezing carrying rod is clamped in the first clamping groove, the open end of the freezing carrying rod is located in the containing groove, the sleeve is clamped in the second clamping groove, and the open end of the freezing carrying rod is located in the containing groove, so that the freezing carrying rod is convenient to store, and pollution is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to embryo experimental equipment technical field, especially a kind of special culture dish for embryo freezing. BACKGROUND

[0002] When carrying out embryo freezing, embryo is usually placed in specific cryoprotective fluid to prevent water in cells from forming ice crystals and causing damage to cell structure. Subsequently, these embryos are loaded onto a freezing carrier rod for rapid cooling to an ultra-low temperature environment, commonly in a liquid nitrogen environment, for long-term preservation.

[0003] During operation, the operator needs to open the sleeve in advance and clamp the freezing carrier rod in the left hand fingers. The left hand needs to operate the freezing plate while clamping the freezing carrier rod, which limits the flexibility of the operator's hand and increases the risk of contamination due to operational errors. SUMMARY

[0004] The utility model aims at providing a special culture dish for embryo freezing, which clamps the freezing carrier rod in the first clamping groove and the sleeve in the second clamping groove, making it convenient for the operator to operate.

[0005] The above technical purpose of the utility model is achieved by the following technical solutions:

[0006] A special culture dish for embryo freezing includes a culture dish, a plurality of first storage holes are provided on the left side of the upper end of the culture dish, a plurality of second storage holes are provided on the right side of the upper end of the culture dish, the diameter of the first storage hole is larger than that of the second storage hole, a placing groove is provided on the rear side of the upper end of the culture dish, a first clamping groove extending to the outside of the culture dish is provided on the left end of the placing groove, a second clamping groove extending to the outside of the culture dish is provided on the right end of the placing groove, and the first clamping groove and the second clamping groove are parallel in front and back.

[0007] By adopting the above technical solutions, the first storage hole stores ES liquid, the second storage hole stores VS liquid, the freezing carrier rod is clamped in the first clamping groove, and the sleeve is clamped in the second clamping groove, making it convenient for the operator to perform embryo freezing operation.

[0008] The utility model further provides that the volume of the first storage hole is 300 μl, and the volume of the second storage hole is 200 μl.

[0009] By adopting the above technical solutions, the first storage hole and the second storage hole are quantitatively because the usage of ES liquid and VS liquid is different, which is beneficial to save materials.

[0010] A further feature of this invention is that the first storage hole and the second storage hole are both arranged in a fan shape, and a partition groove is provided at the upper end of the culture dish between the first storage hole and the second storage hole.

[0011] By adopting the above technical solution, the first storage hole and the second storage hole are evenly distributed, which facilitates operation, while the separator can separate the first storage hole and the second storage hole to avoid mixing.

[0012] A further feature of this invention is that the upper end of the culture dish is provided with a cover.

[0013] By adopting the above technical solution, it is convenient to protect ES liquid and VS liquid.

[0014] A further feature of this invention is that the lower end of the culture dish has a hollow structure.

[0015] By adopting the above technical solutions, manufacturing materials can be saved and costs can be reduced.

[0016] In summary, this utility model has the following beneficial effects:

[0017] Firstly, when this utility model is in use, after the freezing rod is opened, the freezing rod is clamped in the first slot, with the open end located in the placement slot, and the sleeve is clamped in the second slot, with the open end located in the placement slot, which facilitates the storage of the freezing rod and avoids contamination.

[0018] Secondly, the first storage hole and the second storage hole in this utility model are set separately, which makes them easy to distinguish and avoids them being used interchangeably. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a diagram showing the usage state of this utility model.

[0021] In the diagram: 1. Petri dish; 2. First storage hole; 3. Second storage hole; 4. Placement slot; 5. First slot; 6. Second slot; 7. Divider slot; 8. Cover. Detailed Implementation

[0022] The present invention will be further described in detail below with reference to the accompanying drawings.

[0023] In the description of the utility model, it is necessary to understand that the terms "upper", "lower", "left", "right", "front", "rear", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are merely for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model.

[0024] In addition, the terms "horizontal", "vertical" and the like do not mean that the components must be absolutely horizontal or vertical, but can be slightly inclined. For example, "horizontal" only means that it is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0025] In the description of the utility model, it is necessary to understand that the terms "set", "connected", "connected" should be understood broadly unless otherwise specified and limited, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0026] Embodiment, a kind of for embryo freezing special culture dish, as shown in Figure 1 And Figure 2 As shown, it includes culture dish 1, the left side of the upper end of culture dish 1 is provided with a plurality of first storage holes 2, the right side of the upper end of culture dish 1 is provided with a plurality of second storage holes 3, the diameter of first storage hole 2 is greater than the diameter of second storage hole 3, which facilitates the distinction between first storage hole 2 and second storage hole 3 during operation, first storage hole 2 is used to place ES liquid, and second storage hole 3 is used to place VS liquid. A placing groove 4 is formed at the rear side of the upper end of culture dish 1, a first clamping groove 5 extending to the outside of culture dish 1 is formed at the left end of placing groove 4, a second clamping groove 6 extending to the outside of culture dish 1 is formed at the right end of placing groove 4, and the first clamping groove 5 and the second clamping groove 6 are parallel to each other. The first clamping groove 5 and the second clamping groove 6 facilitate the clamping of the freezing carrier rod and the sleeve, after the sleeve on the freezing carrier rod is opened, the freezing carrier rod and the sleeve are fixed parallel to each other, and can be fixed on the culture dish 1, which facilitates subsequent operation.

[0027] The volume of the first storage hole 2 is 300 μl, and the volume of the second storage hole 3 is 200 μl. The first storage hole 2 is used to place ES liquid, and the second storage hole 3 is used to place VS liquid, and the use amount of the two is different, and the fixed volume can avoid material waste and save cost.

[0028] The first storage hole 2 and the second storage hole 3 are both in a fan shape, which is beneficial to the operation of the operator, and a separation groove 7 is arranged between the first storage hole 2 and the second storage hole 3 at the upper end of the culture dish 1, so as to further distinguish the first storage hole 2 and the second storage hole 3 and avoid mixing use.

[0029] A cover 8 is arranged at the upper end of the culture dish 1, which protects the ES liquid and the VS liquid after being dropped into the first storage hole 2 and the second storage hole 3 respectively, and the cover 8 is balanced at room temperature for at least 30 min.

[0030] Operation method: after the ES liquid and the VS liquid are dropped into the first storage hole 2 and the second storage hole 3 respectively, the cover is closed and balanced at room temperature for 30 min, then the embryo is placed in the ES liquid and balanced for 10 min, in the process, the frozen carrier can be opened and fixed on the culture dish 1, after the water and the cryoprotectant in the cell are fully replaced, the embryo is transferred into the VS liquid and balanced, finally, the embryo is moved to the frozen carrier, the sleeve is closed and then quickly put into the liquid nitrogen for preservation.

[0031] The specific embodiment is only an explanation of the utility model, and is not a limitation of the utility model, and the person skilled in the art can make a modification without creative contribution according to the need after reading the specification, but as long as it is within the scope of the claims of the utility model, it is protected by the patent law.

Claims

1. A culture dish for embryo freezing, comprising a culture dish (1), characterized in that: The upper left side of the culture dish (1) is provided with a plurality of first storage holes (2), the upper right side of the culture dish (1) is provided with a plurality of second storage holes (3), the diameter of the first storage hole (2) is larger than that of the second storage hole (3), the upper rear side of the culture dish (1) is provided with a placing groove (4), the left end of the placing groove (4) is provided with a first clamping groove (5) extending to the outside of the culture dish (1), the right end of the placing groove (4) is provided with a second clamping groove (6) extending to the outside of the culture dish (1), and the first clamping groove (5) and the second clamping groove (6) are parallel front and back.

2. The culture dish for embryo freezing according to claim 1, wherein: The volume of the first storage hole (2) is 300ul, and the volume of the second storage hole (3) is 200ul.

3. The culture dish for embryo freezing according to claim 2, wherein: The first storage hole (2) and the second storage hole (3) are both fan-shaped, and the upper end of the culture dish (1) is provided with a separation groove (7) between the first storage hole (2) and the second storage hole (3).

4. The culture dish for embryo freezing according to claim 3, wherein: The upper end of the culture dish (1) is provided with a cover (8).

5. The culture dish for embryo freezing according to claim 4, wherein: The lower end of the culture dish (1) is a hollow structure.

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