Device for uniformly mixing materials

Abstract

The utility model relates to a material mixing device, and belongs to the field of material mixing devices. The device comprises a stirring barrel, a stirrer and a height adjuster, the height adjuster is arranged on the top of the stirring barrel, the stirrer is arranged on the height adjuster, and the height position of the stirrer in the stirring barrel is controlled through the height adjuster. The material uniform mixing device is easy to operate and low in manufacturing cost, an operator can conveniently adjust the height positions of the first cover plate and the second cover plate on the supporting rod by arranging a locking linear bearing with a clamping handle so as to drive the stirrer to carry out vertical height adjustment, so that the height adjustment of the stirring rod in the stirring barrel is realized, and the stirring efficiency is improved. Therefore, materials can be better stirred by adjusting the height of the stirring rod.

Description

TECHNICAL FIELD

[0001] The application relates to a material mixing device. BACKGROUND

[0002] Sepharose6FF gel is a gel filtration filler formed by cross-linking 6% agarose, has good physical stability and chromatographic quality, and has a separation range of 0.01 MD to 1 MD of biological macromolecules such as proteins, polysaccharides and nucleic acids, and can effectively separate and purify biological macromolecules according to the size difference.

[0003] The Sepharose6FF gel is stirred uniformly with the buffer to prepare a uniform gel suspension. The stirring process can eliminate the phenomenon of gel aggregation or local concentration unevenness, so that the gel particles are uniformly distributed in the buffer, a uniform column bed is formed, liquid uniform flow is ensured, resolution is improved, recovery rate is ensured, experimental reproducibility is enhanced, chromatographic efficiency is improved, and the gel concentration is adjusted, and the operation is convenient, thereby laying a good foundation for subsequent separation and purification work.

[0004] However, the current Sepharose6FF gel stirring process with buffer is mostly manual stirring. Manual stirring has the problems of unevenness, low efficiency, high labor intensity, pollution risk and poor repeatability when preparing Sepharose6FF gel suspension. These problems will result in poor stirring effect of Sepharose6FF gel, which will affect the reliability and efficiency of subsequent experimental results. SUMMARY

[0005] To solve or partially solve the problems in the related art, the application provides a material mixing device which can uniformly stir Sepharose6FF gel and buffer, ensure the stirring effect of Sepharose6FF gel, and ensure the reliability and efficiency of subsequent experimental results.

[0006] The material mixing device in the application comprises a stirring barrel, a stirrer and a height adjuster, the height adjuster is arranged on the top of the stirring barrel, the stirrer is arranged on the height adjuster, and the height position of the stirrer in the stirring barrel is controlled by the height adjuster; the top of the stirring barrel is provided with a flange plate; the height adjuster comprises a first cover plate, a second cover plate and a support rod.

[0007] The support rods are fixedly installed on the flange plate through inner and outer tooth extension rods; linear bearings are installed on the first cover plate, the number of the linear bearings corresponds to the number of the support rods, the support rods are connected and installed with the first cover plate through the linear bearings, and the linear bearings are linear bearings locked by clamping handles, so that the height of the first cover plate on the support rods is adjusted;

[0008] Linear bearings are installed on the second cover plate, the number of the linear bearings corresponds to the number of the support rods, the support rods are connected and installed with the second cover plate through the linear bearings, and the linear bearings are linear bearings locked by clamping handles, so that the height of the second cover plate on the support rods is adjusted.

[0009] Optionally, in some embodiments of the present application: further comprising: a first laser and a second laser, the first laser and the second laser are both laser diffuse reflection sensors, the first laser and the second laser are arranged on the side wall of the stirring barrel, and are used to detect the mixing condition of the material in the stirring barrel.

[0010] Optionally, in some embodiments of the present application: the first laser is arranged at a position of 80% of the liquid level height of the material in the stirring barrel; and the second laser is arranged at a position of 15% of the liquid level height of the material in the stirring barrel.

[0011] Optionally, in some embodiments of the present application: a first outer spherical bearing seat is arranged at the center of the first cover plate; a second outer spherical bearing seat is arranged at the center of the second cover plate; the height between the first cover plate and the second cover plate is adjusted by shaft locking bolts on the first outer spherical bearing seat and the second outer spherical bearing seat; the stirrer comprises: a speed regulating motor and a stirring rod, the speed regulating motor is connected and installed with the stirring rod through a shaft coupling, the speed regulating motor is arranged on the top of the second cover plate, and the stirring rod penetrates into the interior of the stirring barrel through the first outer spherical bearing seat and the second outer spherical bearing seat.

[0012] Optionally, in some embodiments of the present application: the stirring rod is made of stainless steel, and a polytetrafluoroethylene shell is wrapped outside the stirring rod, and the wrapping range is from 30cm below the top of the stirring rod to the bottom of the stirring rod.

[0013] Three groups of stirring impellers are arranged on the stirring rod, the stirring impellers are made of polytetrafluoroethylene, one group of stirring impellers is fixedly installed at the top of the stirring rod, and the other two groups of stirring impellers are movably installed on the stirring rod through impeller fixers, and the distance between each group of stirring impellers is not less than 15cm.

[0014] Optionally, in some embodiments of the present application: the stirring impeller is provided with three stirring blades on the circumference, the stirring blades are inclined at an angle of 15° to the rotation direction, the stirring blades are inclined at an angle of 5° to the horizontal plane, and the blade surface of the stirring blade is arc-shaped.

[0015] Optionally, in some embodiments of the present application: the support rod is provided with a scale value; and the support rod is provided with a limiting block to limit the lowest height position of the stirring rod.

[0016] Optionally, in some embodiments of the present application: the bottom of the stirring barrel is provided with a movable support frame; and the bottom of the movable support frame is provided with a universal wheel.

[0017] The present application has the following beneficial effects:

[0018] The material mixing device of the present application is simple to operate and low in manufacturing cost. By arranging the straight line bearing locked by the clamping handle, the operator can conveniently adjust the height position of the first cover plate and the second cover plate on the support rod to drive the stirrer to adjust the height, thereby adjusting the height of the stirring rod in the stirring barrel to better stir the material by adjusting the height of the stirring rod. BRIEF DESCRIPTION OF DRAWINGS

[0019] The above and other objects, features and advantages of the present application will become more apparent from the following detailed description thereof taken in conjunction with the accompanying drawings, in which like reference characters designate the same or similar parts throughout the drawings, and in which:

[0020] Figure 1 is a structural schematic view of a material mixing method in the embodiments of the present application;

[0021] Figure 2 is a structural schematic view of a material mixing device in the embodiments of the present application. DETAILED DESCRIPTION

[0022] The embodiments of the present application will be described in more detail by referring to the embodiments. Although the embodiments show the embodiments of the present application, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to make the present application more thorough and complete, and to fully convey the scope of the present application to those skilled in the art.

[0023] At present, the process of stirring Sepharose 6FF gel with buffer is mostly manually stirred by hand. Manual stirring has problems of unevenness, low efficiency, high labor intensity, pollution risk and poor repeatability when preparing Sepharose 6FF gel suspension, which will cause poor stirring effect of Sepharose 6FF gel and affect the reliability and efficiency of subsequent experimental results.

[0024] In view of the above problems, the application provides a material mixing method, which can uniformly stir Sepharose 6FF gel with buffer, guarantee the stirring effect of Sepharose 6FF gel, and guarantee the reliability and efficiency of subsequent experimental results.

[0025] The technical solutions of the embodiments of the application are described in detail below with reference to the drawings.

[0026] Figure 1 is a structural schematic diagram of the material mixing method in the embodiments of the application;

[0027] Figure 2 is a structural schematic diagram of the material mixing device in the embodiments of the application.

[0028] Referring to Figures 1-2 A material mixing method, applied to a material mixing device, the material mixing device comprising: a stirring barrel 1, a stirrer 2 and a height adjuster 3, the height adjuster 3 is arranged and installed at the top of the stirring barrel 1, the stirrer 2 is arranged and installed on the height adjuster 3, and the height position of the stirrer 2 in the stirring barrel 1 is controlled through the height adjuster 3.

[0029] Specifically, the top of the stirring barrel 1 is provided with a flange plate 101, and the flange plate 101 is made of stainless steel.

[0030] The height adjuster 3 comprises: a first cover plate 301, a second cover plate 302 and a support rod 303, and the first cover plate 301, the second cover plate 302 and the support rod 303 are all made of stainless steel.

[0031] The support rod 303 is provided with three support rods 303, and the support rod 303 is fixedly installed on the flange plate 101 through an inner and outer tooth extension rod.

[0032] The first cover plate 301 is provided with a linear bearing, the number of the linear bearings corresponds to the number of the support rods 303, the support rod 303 is connected and installed with the first cover plate 301 through the linear bearing, and the linear bearing is a linear bearing 306 with a clamping handle lock, so as to realize the height adjustment of the first cover plate 301 on the support rod 303.

[0033] The second cover plate 302 is provided with a linear bearing, the number of the linear bearing corresponds to the number of the support rod 303, the support rod 303 is connected and installed through the linear bearing and the second cover plate 302, and the linear bearing is a linear bearing with a clamping handle lock 306, so as to realize the height adjustment of the second cover plate 302 on the support rod 303.

[0034] Specifically: the center of the first cover plate 301 is provided with a first outer spherical bearing seat 304, and the first outer spherical bearing seat 304 is arranged and installed at the top center of the first cover plate 301; the center of the second cover plate 302 is provided with a second outer spherical bearing seat 305, and the second outer spherical bearing seat 305 is arranged and installed at the bottom center of the second cover plate 302.

[0035] The stirrer 2 comprises a speed regulating motor 201 and a stirring rod 202, the speed regulating motor 201 is connected and installed with the stirring rod 202 through a shaft coupling, the speed regulating motor 201 is arranged and installed at the top of the second cover plate 302, and the stirring rod 202 penetrates through the first outer spherical bearing seat 304 and the second outer spherical bearing seat 305 and extends into the interior of the stirring barrel 1.

[0036] In the embodiment, by arranging the linear bearing with a clamping handle lock 306, the operator can conveniently adjust the height position of the first cover plate 301 and the second cover plate 302 on the support rod 303, so as to drive the stirring rod 202 to realize the height adjustment, thereby realizing the height adjustment of the stirring rod 202 in the stirring barrel 1, and realizing the better stirring of the material by adjusting the height of the stirring rod 202.

[0037] Specifically: the stirring rod 202 is made of stainless steel, and a polytetrafluoroethylene shell is wrapped outside the stirring rod 202, and the wrapping range is from 30cm below the top of the stirring rod 202 to the bottom of the stirring rod 202.

[0038] In the embodiment, the stirring rod 202 made of stainless steel can be connected and installed with the speed regulating motor 201 through the shaft coupling, and the polytetrafluoroethylene shell wrapped at the lower part of the stirring rod 202 can effectively prevent the corrosion of the material to the stirring rod 202 during stirring, and can also avoid the damage of the stirring barrel 1 when the stirring rod 202 collides with the stirring barrel 1.

[0039] The stirring rod 202 is provided with three groups of stirring impellers 203, the stirring impellers 203 are made of polytetrafluoroethylene, one group of the stirring impellers 203 is fixedly installed at the top of the stirring rod 202, and the other two groups of the stirring impellers 203 are movably installed on the stirring rod 202 through an impeller fixator, and the spacing between each group of the stirring impellers 203 is 18cm.

[0040] In the embodiment, by adopting three sets of stirring impellers 203, the stirring volume range of the stirrer 2 can be enhanced, and the overall stirring effect and efficiency can be improved.

[0041] Specifically, the stirring impeller 203 is circumferentially provided with three stirring blades, the stirring blades are inclined at an angle of 15° with respect to the rotation direction, the stirring blades are inclined at an angle of 5° with respect to the horizontal plane, and the blade surface of the stirring blade is arc-shaped.

[0042] In the embodiment, the shape of the stirring impeller 203 combines the characteristics of the folding paddle stirrer 2 and the propeller stirrer 2, so that the axial flow range and the circulation amount can be increased, the low blade tip speed and low shear force can be realized, and the stirring efficiency can be further improved.

[0043] Specifically, the support rod 303 is provided with a scale value.

[0044] The support rod 303 is provided with a limiting block 307 to limit the lowest height position of the stirring rod 202.

[0045] In the embodiment, by providing the scale value on the support rod 303, the operator can conveniently and accurately adjust the height of the stirring rod 202. Meanwhile, the limiting block 307 can limit the descending height of the second cover plate 302, so as to effectively prevent the height of the stirring rod 202 from being too low and avoid the direct contact between the stirring impeller 203 and the container bottom of the stirring barrel 1.

[0046] Specifically, the barrel wall of the stirring barrel 1 is made of transparent glass material.

[0047] The material mixing device further comprises a first laser 4 and a second laser 5, both of which are laser diffuse reflection sensors, and are arranged on the side wall of the stirring barrel 1 to detect the mixing condition of the material in the stirring barrel 1. The first laser 4 and the second laser 5 are respectively installed in a pearl cotton cylinder, the sensor probe protrudes 2 mm from the pearl cotton cylinder, then the pearl cotton cylinder is inserted into a stainless steel pipe with a diameter of 20 mm, the length of the stainless steel pipe is 200 mm, the end of the pearl cotton cylinder is flush with the pipe opening, and finally the stainless steel pipe is installed on the support of the stirring barrel 1 perpendicular to the surface of the column glass barrel, and the pipe opening of the stainless steel pipe is close to the barrel wall surface.

[0048] Specifically, the first laser 4 is arranged at the position of 80% of the liquid level height of the material in the stirring barrel 1, and the second laser 5 is arranged at the position of 15% of the liquid level height of the material in the stirring barrel 1.

[0049] In the embodiment, the barrel wall of the stirring barrel 1 is made of transparent glass material, so that the operator can intuitively observe the material state in the stirring process. Meanwhile, by setting the first laser 4 and the second laser 5, the laser diffuse reflection values of the upper and lower solution layers of the stirring barrel 1 can be detected, and the operator can monitor the mixing condition of the material in the mixing barrel.

[0050] Specifically, the bottom of the stirring barrel 1 is provided with a movable support frame 102 mounted thereon, and the movable support frame 102 is connected with the flange plate 101 through a stainless steel rod.

[0051] The bottom of the movable support frame 102 is provided with universal wheels 103.

[0052] In the embodiment, the movable support frame 102 mounted at the bottom of the stirring barrel 1 can provide stable support and protection for the stirring barrel 1. Meanwhile, the universal wheels 103 provided at the bottom of the movable support frame 102 can facilitate the operator to move the stirring barrel 1 to different positions to adapt to different working requirements.

[0053] The method for mixing the material in the embodiment includes the following steps.

[0054] S100: Step 1: After the material is added into the stirring barrel 1, the stirrer 2 is adjusted to a first height position through the height adjuster 3, then the stirrer 2 is controlled to start stirring, and after the stirrer 2 is started to stir for at least 3 minutes, the stirrer 2 is controlled to stop, wherein the first height position is the height position of the surface of the settled gel in the stirring barrel 1.

[0055] Specifically, after the material is added into the stirring barrel 1, the stirrer 2 is adjusted to a first height position through the straight line bearing 306 with a clamping handle lock on the height adjuster 3, then the stirrer 2 is controlled to start stirring, and the rotating speed of the speed regulation motor 201 is set to 85 r / min, and after the stirrer 2 is started to stir for 3 minutes, the stirrer 2 is controlled to stop, wherein the first height position is the height position of the surface of the settled gel in the stirring barrel 1.

[0056] In the embodiment, by stirring at the height position of the surface of the settled gel, the gel material and the buffer can be fully contacted and mixed in the stirring process, the mixing efficiency is improved, and the generation of air bubbles is effectively reduced, so that the influence of the air bubbles on the mixing effect is avoided.

[0057] S200: Step 2: After the stirrer 2 is stopped, the height of the stirrer 2 is adjusted to be lowered through the height adjuster 3, then the stirrer 2 is controlled to start stirring, and after the stirrer 2 is started to stir for at least 3 minutes, the stirrer 2 is controlled to stop.

[0058] Specifically: after the stirrer 2 is stopped, the height adjustment of the stirrer 2 is reduced by 20cm through the straight line bearing 306 locked by the belt clamping handle on the height adjuster 3, then the stirring of the stirrer 2 is started to be controlled, and the rotating speed of the speed regulation motor 201 is set to 85r / min, after the stirrer 2 is started to stir for 3 minutes, the stirring of the stirrer 2 is stopped to be controlled.

[0059] In the embodiment, by continuously reducing the height of the stirrer 2, the stirrer 2 can process the settled gel layer by layer, improve the liquidity of the material, and ensure that each layer of material can be fully stirred, thereby improving the mixing uniformity of the whole material. At the same time, by setting a reasonable stirring time, the energy consumption and material waste caused by too long stirring time can be avoided, and the efficiency of stirring is effectively ensured.

[0060] S300: Step 3: repeat the operation of step 2, continuously reduce the height adjustment of the stirrer 2 and control the stirring, until the stirrer 2 is adjusted to a second height position, which is at least 5cm from the bottom of the stirring barrel 1, after the stirrer 2 is started to stir for at least 3 minutes, the stirring of the stirrer 2 is stopped to be controlled, and the mixing operation of the material is completed.

[0061] Specifically: repeat the operation of step 2, continuously reduce the height adjustment of the stirrer 2 and control the stirring, until the stirrer 2 is adjusted to a height position of about 5cm from the bottom of the stirring barrel 1, and the stirring of the stirrer 2 is started to be controlled;

[0062] The first laser 4 and the second laser 5 are controlled to detect, and first detection value and second detection value are obtained;

[0063] The difference between the first detection value and the second detection value is judged, if the difference between the first detection value and the second detection value meets the preset difference value range, the stirring of the stirrer 2 is stopped to be controlled, and the mixing operation of the material is completed.

[0064] In the embodiment, by judging the difference between the first detection value and the second detection value, the operator can effectively evaluate the mixing uniformity of the material, so as to ensure that the material mixing effect meets the standard, and the mixing uniformity of the final material is ensured.

[0065] The technical scheme of the embodiment of the application includes the following beneficial effects:

[0066] The method for mixing materials can make the gel material and the buffer fully contact and mix during the stirring process, improve the mixing efficiency, and improve the flowability of the material, so that each layer of material can be fully stirred, thereby improving the mixing uniformity of the overall material.

[0067] The device for mixing materials is simple to operate and low in manufacturing cost. By arranging the linear bearing 306 locked by the clamping handle, the operator can conveniently adjust the height position of the first cover plate 301 and the second cover plate 302 on the support rod 303, so as to drive the stirrer 2 to adjust the height, thereby adjusting the height of the stirring rod 202 in the stirring barrel 1, and better stirring the material by adjusting the height of the stirring rod 202.

[0068] The above has described the embodiments of the present application, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes are obvious to those skilled in the art without departing from the scope and spirit of the described embodiments. The selection of the terms used herein is intended to best explain the principles, practical application, or improvement of the technology in the market of the embodiments, or to enable other ordinary skilled persons in the art to understand the embodiments disclosed herein.

Claims

1. A device for mixing material, characterized in that, The utility model relates to a kind of mixing barrel, mixing device and height regulator, including: Mixing barrel (1), stirrer (2) and height regulator (3), the height regulator (3) is arranged to install in the top of the mixing barrel (1), the stirrer (2) is arranged to install on the height regulator (3), the height position of stirrer (2) in mixing barrel (1) is controlled by height regulator (3);The top of the mixing barrel (1) is provided with flange (101);The height regulator (3) includes: first cover plate (301), second cover plate (302) and support rod (303); The support rod (303) is provided with at least three, support rod (303) is fixedly installed on the flange (101) by inside and outside tooth extension rod;Linear bearing is arranged and installed on the first cover plate (301), the number of linear bearing corresponds the number of support rod (303), support rod (303) passes through linear bearing and is connected and installed with the first cover plate (301), and the linear bearing is locking linear bearing (306) with clamping handle, to realize the height adjustment of the first cover plate (301) on support rod (303); Linear bearing is arranged and installed on the second cover plate (302), the number of linear bearing corresponds the number of support rod (303), support rod (303) passes through linear bearing and is connected and installed with the second cover plate (302), and the linear bearing is locking linear bearing (306) with clamping handle, to realize the height adjustment of the second cover plate (302) on support rod (303).

2. A material mixing apparatus as claimed in claim 1, wherein Also including: First laser (4) and second laser (5), the first laser (4) and second laser (5) are all laser diffuse reflection sensors, the first laser (4) and second laser (5) are arranged in the side wall of the mixing barrel (1), for detecting the mixing condition of material in mixing barrel (1).

3. A material mixing apparatus as claimed in claim 2, wherein The first laser (4) is arranged and installed at the 80% position of material liquid level height in the mixing barrel (1);The second laser (5) is arranged and installed at the 15% position of material liquid level height in the mixing barrel (1).

4. A material mixing apparatus as claimed in claim 1, wherein The center of the first cover plate (301) is provided with first outer spherical bearing seat (304), and the center of the second cover plate (302) is provided with second outer spherical bearing seat (305);The height between the first cover plate (301) and the second cover plate (302) is adjusted by shaft locking bolt on first outer spherical bearing seat (304) and second outer spherical bearing seat (305);The stirrer (2) includes: speed regulating motor (201) and stirring rod (202), the speed regulating motor (201) is installed and connected with the stirring rod (202) by coupling, the speed regulating motor (201) is arranged and installed in the top of the second cover plate (302), and the stirring rod (202) passes through first outer spherical bearing seat (304) and second outer spherical bearing seat (305) and extends into the inside of the mixing barrel (1).

5. A material mixing apparatus as claimed in claim 4, wherein The stirring rod (202) is made of stainless steel, and a polytetrafluoroethylene shell is wrapped outside the stirring rod (202), and the wrapping range is from 30 cm below the top of the stirring rod (202) to the bottom of the stirring rod (202); Three groups of stirring impellers (203) are arranged on the stirring rod (202), the stirring impellers (203) are made of polytetrafluoroethylene, one group of stirring impellers (203) is fixedly installed on the top of the stirring rod (202), and the other two groups of stirring impellers (203) are movably installed on the stirring rod (202) through impeller fixers, and the spacing between each group of stirring impellers (203) is not less than 15 cm.

6. A material mixing apparatus as claimed in claim 5, wherein Three stirring blades are circumferentially arranged on the stirring impeller (203), the stirring blades are inclined at an angle of 15° with respect to the rotating direction, the stirring blades are inclined at an angle of 5° with respect to the horizontal plane, and the blade surface of the stirring blade is arc-shaped.

7. A material mixing apparatus as claimed in claim 5, wherein A scale value is arranged on the supporting rod (303), and a limiting clamping block (307) is arranged on the supporting rod (303) to limit the lowest height position of the stirring rod (202).

8. The apparatus for mixing a material as recited in claim 1, wherein An active supporting frame (102) is arranged at the bottom of the stirring barrel (1), and universal wheels (103) are arranged at the bottom of the active supporting frame (102).

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