Nucleic acid extraction amplification detection kit

Abstract

The utility model discloses a nucleic acid extraction and amplification detection kit which comprises a shell, a reaction tank is fixedly connected to the inner wall of the shell, a top cover is arranged at the top of the shell, a fixed pipe is fixedly connected to the bottom of the top cover, and a sealing cover for sealing the reaction tank is fixedly connected to the bottom of the fixed pipe; the top of the top cover is fixedly connected with a motor, one end of an output shaft of the motor is fixedly connected with a driving shaft, and the driving shaft rotationally penetrates through the sealing cover. According to the reaction tank disclosed by the utility model, a solution can be mixed through the stirring blades and is matched with the fixed cylinder, so that the solution can circularly flow, the heating or annealing uniformity is further improved, the test effect is further improved, the temperature detection accuracy is also improved, and the temperature can be accurately detected in the heating and cooling processes of the reaction tank. Air can enter the reaction tank, so that the reaction tank is prevented from being damaged due to the influence of air pressure, the use safety is improved, the interior of the reaction tank is conveniently cleaned, and people can conveniently use the reaction tank.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological detection technical field especially relates to a nucleic acid extraction amplification detection kit. BACKGROUND

[0002] Nucleic acid amplification detection is the method that through the action of enzyme, the nucleic acid sequence of the detection is amplified, and then detected.

[0003] Through the retrieval, the nucleic acid extraction amplification detection kit of a kind of nucleic acid extraction amplification detection kit is disclosed in the announcement No. UTILITY MODEL CONTENT

[0004] The utility model aims at solving the shortcomings in the prior art and provides a nucleic acid extraction amplification detection kit.

[0005] To achieve the above object, the utility model adopts the following technical scheme:

[0006] A nucleic acid extraction amplification detection kit, comprising a casing, the inner wall of the casing is fixedly connected with a reaction tank, the top of the casing is provided with a top cover, the bottom of the top cover is fixedly connected with a fixed tube, the bottom of the fixed tube is fixedly connected with a sealing cover for closing the reaction tank;

[0007] The top of the top cover is fixedly connected with a motor, one end of the motor output shaft is fixedly connected with a driving shaft, the driving shaft rotates through the sealing cover, the driving shaft is fixedly connected with helical stirring blade, the bottom inner wall of the reaction tank is fixedly connected with a fixed cylinder, the bottom of the fixed cylinder and the bottom inner wall of the reaction tank are provided with a gap, the stirring blade is located in the fixed cylinder, one side of the bottom of the reaction tank is fixedly connected with a discharge pipe, and the discharge pipe passes through the casing;

[0008] The top of the top cover is fixedly connected with a plurality of solvent pipes, and the bottom of the solvent pipe passes through the sealing cover.

[0009] Refrigeration mechanism, the refrigeration mechanism is arranged at the bottom of reaction tank for controlling temperature in reaction tank.

[0010] As a further scheme of the utility model, the refrigeration mechanism includes a semiconductor refrigeration sheet and a heat conduction plate, the semiconductor refrigeration sheet is fixed on the inner wall of the shell, the heat conduction plate is fixed on the bottom of the reaction tank, the surface of the semiconductor refrigeration sheet is in contact with the heat conduction plate, and the bottom of the semiconductor refrigeration sheet is fixedly connected with a plurality of heat dissipation fins.

[0011] As a further scheme of the utility model, the circumference of the shell is fixedly connected with an air inlet pipe and an air outlet pipe, two heat dissipation fans are fixedly installed in the air outlet pipe.

[0012] As a further scheme of the utility model, a filter screen for filtering air is fixedly installed in the air inlet pipe.

[0013] As a further scheme of the utility model, the top of the sealing cover is fixedly installed with a penetrating communication pipe, the top and bottom diameters of the communication pipe are larger than the middle diameter, a plug is slidably connected in the communication pipe, the plug is located in the middle of the communication pipe, and springs are fixedly connected between the top and bottom inner walls of the communication pipe and the plug.

[0014] As a further scheme of the utility model, two obliquely arranged scrapers are fixedly connected to the circumference of the drive shaft, and the scrapers are in contact with the inner wall of the reaction tank.

[0015] As a further scheme of the utility model, a controller is fixedly connected to the bottom inner wall of the shell, a display is fixedly connected to the side outer wall of the shell, a temperature sensor is installed on the bottom inner wall of the reaction tank, and the display, the controller and the temperature sensor are electrically connected.

[0016] The utility model discloses the beneficial effects are:

[0017] 1. Through the setting of installing the stirring vane in the reaction tank, after sample and solvent are put into the reaction tank, the solution can be mixed through the stirring vane, and the fixed cylinder is cooperated, so that the solution can circulate and flow, and then the uniformity of heating or annealing is improved, and then the test effect is increased, and the accuracy of temperature detection is increased.

[0018] 2. Through the cooperation between the communication pipe and the plug, in the process that the reaction tank is heated and cooled, air can enter the reaction tank, the reaction tank is prevented from being damaged due to air pressure, and the safety in use is improved.

[0019] 3. Through the setting that the top cover and the sealing cover can be removed, after the equipment is used, the top cover and the sealing cover can be conveniently removed, then the inside of the reaction tank is conveniently cleaned, and people are conveniently used. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1A kind of nucleic acid extraction amplification detection kit's front view structure schematic diagram is proposed in the utility model;

[0021] Figure 2 A kind of nucleic acid extraction amplification detection kit's rear view structure schematic diagram is proposed in the utility model;

[0022] Figure 3 A kind of nucleic acid extraction amplification detection kit's section view structure schematic diagram is proposed in the utility model;

[0023] Figure 4 A kind of nucleic acid extraction amplification detection kit's top cover section view structure schematic diagram is proposed in the utility model;

[0024] Figure 5 A kind of nucleic acid extraction amplification detection kit's Figure 4 A point amplification structure schematic diagram in the utility model.

[0025] In the drawing: 1, casing;2, top cover;3, solvent pipe;4, air inlet pipe;5, filter screen;6, discharge pipe;7, display;8, air outlet pipe;9, motor;10, fixed pipe;11, sealing cover;12, drive shaft;13, reaction tank;14, fixed cylinder;15, semiconductor refrigeration sheet;151, radiating fin;16, controller;17, Radiator fan;18, scraper;19, stirring blade;20, communication pipe;21, plug;22, spring. DETAILED DESCRIPTION

[0026] The technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. It should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "setting" should be understood broadly, and the specific meanings of the above terms in the patent can be understood by those skilled in the art according to the specific circumstances.

[0027] Referring to Figures 1-5 A kind of nucleic acid extraction amplification detection kit, including casing 1, the inner wall of casing 1 is fixedly connected with reaction tank 13, the top of casing 1 is provided with top cover 2, top cover 2 and casing 1 are connected by quick lock (not shown in the drawing), the two sides of top cover 2 are also installed with guide rod, the two sides of casing 1 are fixed with guide sleeve, two guide rods are respectively inserted with two guide sleeves, the bottom of top cover 2 is fixedly connected with fixed pipe 10, the bottom of fixed pipe 10 is fixedly connected with the sealing cover 11 for closing reaction tank 13;

[0028] The top of the top cover 2 is fixedly connected with a motor 9, one end of the output shaft of the motor 9 is fixedly connected with a driving shaft 12, the driving shaft 12 rotates and penetrates the sealing cover 11, the circumference of the driving shaft 12 is fixedly connected with helical stirring blades 19, the bottom inner wall of the reaction tank 13 is fixedly connected with a fixed cylinder 14, a gap is arranged between the bottom of the fixed cylinder 14 and the bottom inner wall of the reaction tank 13, the stirring blades 19 are located in the fixed cylinder 14, one side of the bottom of the reaction tank 13 is fixedly connected with a discharge pipe 6, the discharge pipe 6 penetrates the casing 1, the motor 9 drives the driving shaft 12 to rotate, the driving shaft 12 drives the stirring blades 19 to mix the liquid, improves the mixing effect, cooperates with the fixed cylinder 14, so that the solution can flow circularly, and then the uniformity of heating or annealing is improved, and then the test effect is increased, and the accuracy of temperature detection is increased.

[0029] A plurality of solvent pipes 3 are fixedly connected to the top of the top cover 2, the top of the plurality of solvent pipes 3 is provided with a cover plate, so as to prevent the solvent pipes 3 from being polluted, the bottom of the solvent pipes 3 penetrates the sealing cover 11, and the solvent pipes 3 are provided with electromagnetic valves and are used for respectively adding buffer solution, MgCl2, dNTPs, primers, Taq DNA polymerase, template DNA, water and samples.

[0030] The refrigeration mechanism is arranged at the bottom of the reaction tank 13 and is used for controlling the temperature in the reaction tank 13.

[0031] In the utility model, the refrigeration mechanism comprises a semiconductor refrigeration piece 15 and a heat conduction plate, the semiconductor refrigeration piece 15 is fixed to the inner wall of the casing 1, the heat conduction plate is fixed to the bottom of the reaction tank 13, the surface of the semiconductor refrigeration piece 15 is in contact with the heat conduction plate, a plurality of heat dissipation fins 151 are fixedly connected to the bottom of the semiconductor refrigeration piece 15, the semiconductor refrigeration piece 15 is prior art, when direct current passes through a couple formed by two different semiconductor materials, heat can be absorbed and heat can be emitted at the two ends of the couple, so that the purpose of refrigeration can be achieved, and the working raw material and the use method will not be repeated here.

[0032] Especially, the circumference of the casing 1 is fixedly connected with an air inlet pipe 4 and an air outlet pipe 8, two heat dissipation fans 17 are fixedly installed in the air outlet pipe 8, in the working process of the semiconductor refrigeration piece 15, the heat dissipation fans 17 are started, the heat dissipation fans 17 extract the heat or cold emitted by the heat dissipation fins 151, and the working of the semiconductor refrigeration piece 15 is facilitated.

[0033] It should be noted that the air inlet pipe 4 is fixedly installed with a filter screen 5 for filtering air, and the filter screen 5 prevents foreign matters from entering the equipment.

[0034] The utility model discloses, the top fixed mounting of sealing cover 11 has the through communication pipe 20, the top and bottom diameter of communication pipe 20 is greater than the middle part diameter, the plug 21 is slidably connected in communication pipe 20, the plug 21 is located in the middle part of communication pipe 20, and the top inner wall and bottom inner wall of communication pipe 20 are all fixedly connected with spring 22 between plug 21, in the process that reaction kettle 13 is in the heating and cooling, air can enter into reaction kettle 13, avoid the damage of reaction kettle 13 to be affected to lead to by air pressure, improve the security of use.

[0035] Especially, the circumferential fixed connection of driving shaft 12 has two obliquely arranged scrapers 18, and the scraper 18 is in contact with the inner wall of the reaction kettle 13, and the scraper 18 can play the effect of stirring, and can reduce the raw materials in the reaction kettle 13 during discharging.

[0036] It should be noted that the bottom inner wall of the casing 1 is fixedly connected with the controller 16, one side of the outer wall of the casing 1 is fixedly connected with the display 7, and the bottom inner wall of the reaction kettle 13 is provided with a temperature sensor, and the display 7, the controller 16 and the temperature sensor are electrically connected.

[0037] Working principle: when using, the sample and reaction solvent are respectively put into the reaction kettle 13 through the solvent pipe 3, and then mixed, when mixing, the motor 9 is started, the motor 9 drives the driving shaft 12 to rotate, the driving shaft 12 drives the stirring blade 19 and the scraper 18 to mix the liquid, improves the mixing effect, at the same time, the semiconductor refrigerating sheet 15 is electrified, the reaction kettle 13 is heated through the semiconductor refrigerating sheet 15, at the same time of heating, the medium in the fixed cylinder 14 is pushed upward by the stirring blade 19, and then the liquid forms circulating flow, improves the uniformity of heating, and then increases the testing effect, at the same time increases the accuracy of temperature detection, after heating, the semiconductor refrigerating sheet 15 is electrified, and then the reaction kettle 13 is cooled and annealed through the semiconductor refrigerating sheet 15, in the annealing process, the circulating flow of the liquid is utilized, the annealing effect and the accuracy of temperature detection are increased, in the working process of the semiconductor refrigerating sheet 15, the cooling fan 17 is started, the cooling fan 17 extracts the heat or cold of the heat dissipation fin 151, facilitates the work of the semiconductor refrigerating sheet 15, after use, the solvent is discharged through the discharge pipe 6, the top cover 2 is removed, the top cover 2 is removed together with the sealing cover 11, and then the inside of the reaction kettle 13 is conveniently cleaned.

[0038] In the application, the structures and connection relations not described in detail are prior art, and the structures and principles are well-known technology, which will not be repeated here.

[0039] The above merely describes a preferred embodiment of the present application, and the protection scope of the present application is not limited thereto, and any skilled person in the art, according to the technical scheme and the inventive concept of the present application, makes equivalent replacement or change within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A nucleic acid extraction amplification test kit comprising a casing (1), characterized in that, The inner wall of the shell (1) is fixedly connected with a reaction tank (13), the top of the shell (1) is provided with a top cover (2), the bottom of the top cover (2) is fixedly connected with a fixed pipe (10), the bottom of the fixed pipe (10) is fixedly connected with a sealing cover (11) for closing the reaction tank (13); The top of the top cover (2) is fixedly connected with a motor (9), one end of the output shaft of the motor (9) is fixedly connected with a driving shaft (12), the driving shaft (12) is rotatably penetrated through the sealing cover (11), the circumference of the driving shaft (12) is fixedly connected with helical stirring blades (19), the bottom inner wall of the reaction tank (13) is fixedly connected with a fixed cylinder (14), a gap is arranged between the bottom of the fixed cylinder (14) and the bottom inner wall of the reaction tank (13), the stirring blades (19) are located in the fixed cylinder (14), one side of the bottom of the reaction tank (13) is fixedly connected with a discharge pipe (6), the discharge pipe (6) penetrates through the shell (1); The top of the top cover (2) is fixedly connected with a plurality of solvent pipes (3), the bottom of the solvent pipe (3) penetrates through the sealing cover (11); A refrigeration mechanism is arranged at the bottom of the reaction tank (13) for controlling the temperature in the reaction tank (13).

2. The nucleic acid extraction and amplification test kit according to claim 1, characterized by, The refrigeration mechanism comprises a semiconductor refrigeration sheet (15) and a heat conduction plate, the semiconductor refrigeration sheet (15) is fixed to the inner wall of the shell (1), the heat conduction plate is fixed to the bottom of the reaction tank (13), the surface of the semiconductor refrigeration sheet (15) is in contact with the heat conduction plate, and the bottom of the semiconductor refrigeration sheet (15) is fixedly connected with a plurality of heat dissipation fins (151).

3. The nucleic acid extraction and amplification test kit according to claim 2, characterized by, The circumference of the shell (1) is fixedly connected with an air inlet pipe (4) and an air outlet pipe (8), two heat dissipation fans (17) are fixedly installed in the air outlet pipe (8).

4. The nucleic acid extraction and amplification test kit according to claim 3, characterized by, A filter screen (5) for filtering air is fixedly installed in the air inlet pipe (4).

5. The nucleic acid extraction and amplification test kit according to claim 1, characterized by, The top of the sealing cover (11) is fixedly installed with a penetrating communication pipe (20), the top and bottom diameters of the communication pipe (20) are larger than the middle diameter, a plug (21) is slidably connected in the communication pipe (20), the plug (21) is located in the middle of the communication pipe (20), springs (22) are fixedly connected between the top and bottom inner walls of the communication pipe (20) and the plug (21).

6. The nucleic acid extraction and amplification test kit according to claim 1, characterized by, The circumference of the driving shaft (12) is fixedly connected with two obliquely arranged scrapers (18), the scrapers (18) are in contact with the inner wall of the reaction tank (13).

7. The nucleic acid extraction and amplification test kit according to claim 1, characterized by, The bottom inner wall of the shell (1) is fixedly connected with a controller (16), one side of the outer wall of the shell (1) is fixedly connected with a display (7), a temperature sensor is installed on the bottom inner wall of the reaction tank (13), and the display (7), the controller (16) and the temperature sensor are electrically connected.

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