Culture device for microscope
By incorporating an independent heating element and a detachable humidification tank into the microscope culture apparatus, the problems of inaccurate temperature and humidity control and difficulty in cleaning the humidification tank are solved, achieving the optimal environment for cell culture and rapid, uniform heating, thus improving the culture effect.
Patent Information
- Application Number
- CN202423262849.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2026-01-20
- Estimated Expiration
- 2034-12-30
AI Technical Summary
Existing microscope culture equipment is not precise in controlling temperature, humidity and gas environment, and the humidification tank is difficult to clean, which affects the cell culture effect.
The design incorporates independent first and second heating components to control the temperature and humidity of the culture dish and humidification tank, respectively. A third heating component is installed on the tank cover to achieve rapid and uniform heating. The humidification tank is detachably connected to the tank body for easy cleaning.
It achieves precise control of temperature and humidity within the culture device, ensuring that cells are cultured in the optimal environment, and solves the cleaning problem of the humidification tank, thereby improving the culture effect.
Smart Images
Figure CN223813513U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture field especially a culture device for microscope. BACKGROUND
[0002] Nowadays, the culture device for microscope has very extensive application in many fields such as life science, pharmacy, cell kinetics research, living cell observation and counting, in-vitro cell culture, drug screening, tissue engineering, living cell detection and the like. Therefore, how to keep the activity of cells in the experimental process and make the cells as close to the natural state as possible has become a problem to be solved for the culture device for microscope.
[0003] In the process of cell culture, temperature, humidity, pH value and the gas environment are very sensitive, but the cell culture device provided by the prior art has many problems such as uneven temperature inside the device, inaccurate temperature control, rough gas concentration control, and non-real-time humidity detection.
[0004] In addition, since the cell culture device is usually provided with a humidifying tank structure, the cell culture device provided by the prior art cannot control the temperature of the humidifying tank separately, which makes it difficult to accurately control the temperature and humidity in the culture device, and there is also a problem that the humidifying tank is difficult to clean. SUMMARY
[0005] To solve the problems in the prior art, the utility model discloses a culture device for microscope.
[0006] A culture device for microscope, comprising a movable connection warehouse body and warehouse cover, the warehouse body is equipped with the placing area for placing the culture dish, is provided with humidifying tank around the placing area, the humidifying tank is detachable with the warehouse body connection;
[0007] The placing area comprises a first heating assembly for heating the culture dish, the humidifying tank comprises a second heating assembly for heating the water in the humidifying tank, the first heating assembly and the second heating assembly are independent of each other, and the third heating assembly is arranged on the warehouse cover.
[0008] Specifically, the cell has higher requirements for temperature and humidity during the culture process, the temperature needs to be maintained by heating the culture dish where the cell is located, and the humidity needs to be maintained by heating the water in the humidifying tank to evaporate, so the same heating assembly cannot control both accurately. Therefore, the first heating assembly and the second heating assembly are arranged to heat the culture dish and the water in the humidifying tank respectively, which can more effectively ensure that the cell is cultured at a suitable temperature and humidity. At the same time, the third heating assembly is arranged on the warehouse cover, which can make the temperature in the culture device rise more rapidly and uniformly.
[0009] In addition, the humidifying tank is detachably connected with the bin body, that is, the humidifying tank can be detached from the bin body for cleaning, thereby solving the problem that the existing culture device is difficult to clean the humidifying tank thoroughly.
[0010] Preferably, the placement area is provided with a placement ring for fixing the position of the culture dish, and a tray is arranged between the placement ring and the bottom plate of the bin body, and the placement ring is detachably connected with the tray, and the tray is used to limit the position of the placement ring.
[0011] Specifically, in order to ensure the stability of the position of the cells in the culture device, it is appropriate to arrange the structure of the placement ring for stabilizing the position of the culture dish, and in addition, since the placement ring is detachably connected with the tray, the placement ring and the tray can be connected according to the size of the culture dish, so as to better fix the culture dish.
[0012] Preferably, the first heating assembly comprises a first heating sheet and a first temperature uniform sheet fixed with the bottom plate of the bin body, and the first heating sheet and the first temperature uniform sheet are uniformly distributed on the entire bottom plate of the bin body, and the first temperature uniform sheet is used to make the temperature of the bottom plate of the bin body uniform.
[0013] Specifically, since the placement ring is movably fixed on the bottom plate of the bin body through the tray, the temperature of the tray and the placement ring is uniform when the temperature of the bottom plate of the bin body is uniform, and then the temperature of the culture dish is also kept in a uniform state, and the cells in the culture dish are also cultured in a temperature uniform and stable state.
[0014] Preferably, the bottom plate of the bin body and the tray are magnetically connected.
[0015] Specifically, the magnetic connection makes the tray and the bottom plate of the bin body in close contact, so that the heat of the bottom plate of the bin body can be smoothly transmitted to the bottom of the tray, and the heat is transmitted to the placement ring and the culture dish from the bottom of the tray.
[0016] Preferably, the second heating assembly comprises a second heating sheet arranged at the bottom of the humidifying tank and a heat preservation and insulation layer, and the heat preservation and insulation layer is arranged between the second heating sheet and the first heating assembly.
[0017] The second heating assembly further comprises a third heating sheet and a second temperature uniform sheet arranged on the side wall of the humidifying tank, and the second temperature uniform sheet is used to make the temperature of the side wall of the humidifying tank uniform.
[0018] Specifically, the heat preservation and insulation layer is used to separate the second heating sheet from the first heating assembly, so as to prevent the mutual influence between the second heating sheet and the first heating assembly from causing negative influence on temperature control.
[0019] The structure that the second heating sheet and the third heating sheet are arranged on the bottom and the side wall of the humidifying tank respectively, realizes multi-surface heating of the water in the humidifying tank, and the multi-surface heating makes the water in the humidifying tank heat up faster and the temperature more uniform.
[0020] Preferably, the third heating assembly comprises a heating glass and a temperature sensor, both of which are arranged on the cover.
[0021] Specifically, the combination of the first heating assembly, the second heating assembly and the third heating assembly realizes multi-surface heating of the culture dish and the water in the humidifying tank, so that the entire culture device has no cold surface, and the internal temperature of the culture device is quickly raised while the influence of external environmental changes on the internal temperature is reduced.
[0022] Preferably, the humidifying tank is open at the top, the side plate of the humidifying tank is provided with a plurality of protrusions at the top, and when the cover is closed, the plurality of protrusions abut against the top of the cover, and a gas flow channel is formed between the top of the side plate of the humidifying tank without protrusions and the cover.
[0023] The humidifying tank is provided with an air inlet and a water inlet, and the air inlet and the water inlet are located outside the tank body.
[0024] Specifically, the gas flow channel formed between the humidifying tank and the heating glass can make the gas in the culture device more evenly distributed, and facilitate rapid replacement of the gas in the culture device.
[0025] Preferably, the cover is further provided with a protective shell for protecting the heating glass, the protective shell is detachably connected with the tank body, and the protective shell is provided with a sealing ring for sealing connection with the tank body.
[0026] Specifically, the cells need to maintain the stability of the environment during the culture process, so they are isolated from the external environment, and the structure of the sealing ring can more effectively isolate the external environment.
[0027] Preferably, it further comprises a humidity sensor for detecting the humidity in the culture device in real time, and the humidity sensor has a reminding function.
[0028] Specifically, the humidity in the culture device directly affects the osmotic pressure of the culture solution, so when the humidity sensor detects that the humidity value in the culture device starts to drop, the reminding function will start to remind the operator to add water to the humidifying tank.
[0029] Preferably, it further comprises a cover state detector for detecting the sealing state between the cover and the tank body.
[0030] Specifically, when the cover state detector detects that the tank body and the cover are in a sealed state, a large amount of gas blowing can be used to quickly replace and adjust the gas in the culture device, so that the gas concentration in the culture device is quickly restored.
[0031] Compared with the prior art, the advantages of this utility model are:
[0032] By setting up a structure where the first heating component and the second heating component heat the water in the culture dish and the humidification tank respectively, the temperature and humidity in the culture device are controlled separately, achieving more precise control of the temperature and humidity in the culture device, so that the cells are cultured in an optimal temperature and humidity environment; the structure combining the first heating component, the second heating component, and the third heating component achieves opposite heating of the water in the culture dish and the humidification tank, making the temperature rise of both faster and more uniform. Attached Figure Description
[0033] Figure 1 This is a three-dimensional schematic diagram of the microscope culture device disclosed in this utility model;
[0034] Figure 2 This is a schematic diagram of the microscope culture device without a chamber cover disclosed in this utility model;
[0035] Figure 3 This is a schematic diagram of the chamber of the microscope culture device disclosed in this utility model;
[0036] Figure 4 This is a schematic diagram of the humidification tank of the microscope culture device disclosed in this utility model;
[0037] Figure 5 This is a full cross-sectional schematic diagram of the microscope culture device disclosed in this utility model. Detailed Implementation
[0038] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0039] like Figures 1-5 As shown, the microscope culture device includes a chamber 10 and a chamber cover 20 that are movably connected. The chamber 10 has a placement area for placing culture dishes, and a humidification tank 30 is arranged around the placement area. The humidification tank 30 is detachably connected to the chamber 10.
[0040] The placement area includes a first heating component for heating the petri dish, the humidification tank 30 includes a second heating component for heating the water in the humidification tank 30, the first heating component and the second heating component are independent of each other, and a third heating component is provided on the cover 20.
[0041] The cells have high requirements for temperature and humidity during the culture process. The temperature is maintained by heating the culture dish where the cells are located, and the humidity is maintained by heating the water in the humidifying tank 30 to evaporate. The same heating assembly cannot accurately control both, so setting the first heating assembly and the second heating assembly to heat the culture dish and the water in the humidifying tank 30 respectively can more effectively ensure that the cells are cultured at a suitable temperature and humidity. At the same time, the third heating assembly structure provided on the cover 20 can make the temperature in the culture device rise more quickly and uniformly.
[0042] In addition, the humidifying tank 30 is detachably connected to the bin body 10, that is, the humidifying tank 30 can be detached from the bin body 10 for cleaning, solving the problem that the existing culture device is difficult to clean the humidifying tank 30 thoroughly.
[0043] The placement area is provided with a placement ring 41 for fixing the position of the culture dish. The placement ring 41 and the tray 42 are detachably connected between the bottom plate of the bin body 10. The tray 42 is used to limit the position of the placement ring 41.
[0044] In order to ensure the stability of the position of the cells in the culture device, it is appropriate to set the structure of the placement ring 41 to stabilize the position of the culture dish. In addition, since the placement ring 41 and the tray 42 are detachably connected, the appropriate placement ring 41 and tray 42 can be selected according to the size of the culture dish to better fix the culture dish.
[0045] The first heating assembly includes a first heating sheet 51 and a first temperature equalizing sheet 52 fixed to the bottom plate of the bin body 10. The first heating sheet 51 and the first temperature equalizing sheet 52 are evenly distributed on the entire bottom plate of the bin body 10. The first temperature equalizing sheet 52 is used to make the temperature of the bottom plate of the bin body 10 uniform.
[0046] Since the placement ring 41 is movably fixed to the bottom plate of the bin body 10 through the tray 42, the temperature of the tray 42 and the placement ring 41 is uniform when the temperature of the bottom plate of the bin body 10 is uniform, which in turn causes the temperature of the culture dish to remain in a uniform state, and the cells in the culture dish are also cultured in a temperature uniform and stable state.
[0047] The middle part of the bottom plate of the bin body 10 is hollowed out (the bottom can be sealed after the tray 42, the placement ring 41 and the culture dish are placed in turn), that is, the first heating assembly provided on the bottom plate of the bin body 10 is a full-angle heating structure around the culture dish.
[0048] The bottom plate of the bin body 10 is magnetically connected to the tray 42.
[0049] The magnetic connection makes the tray 42 and the bottom plate of the cabinet 10 in close contact, so that the heat of the bottom plate of the cabinet 10 can be smoothly transmitted to the bottom of the tray 42, and the bottom of the tray 42 transmits the heat to the culture dish and the culture dish.
[0050] The second heating assembly includes a second heating sheet 61 arranged at the bottom of the humidifying tank 30 and a heat preservation layer 62 arranged between the second heating sheet 61 and the first heating assembly;
[0051] The second heating assembly further includes a third heating sheet 63 arranged on the side wall of the humidifying tank 30 and a second temperature equalizing sheet 64 for equalizing the temperature of the side wall of the humidifying tank 30.
[0052] The heat preservation layer 62 separates the second heating sheet 61 from the first heating assembly, preventing mutual influence between the second heating sheet 61 and the first heating assembly and causing negative effects on temperature control;
[0053] The structure of the second heating sheet 61 and the third heating sheet 63 arranged on the bottom and the side wall of the humidifying tank 30 respectively realizes multi-surface heating of the water in the humidifying tank 30, which makes the water in the humidifying tank 30 heat up faster and more uniform.
[0054] The third heating assembly includes a heating glass 21 and a temperature sensor 22, both of which are arranged on the cabinet cover 20.
[0055] The combination of the first heating assembly, the second heating assembly, and the third heating assembly realizes multi-surface heating of the culture dish and the water in the humidifying tank 30, making the entire culture device have no cold surface and achieving rapid internal heating while reducing the influence of external environmental changes on the internal temperature.
[0056] The humidifying tank 30 has an opening at the top, and the side plate of the humidifying tank 30 has a plurality of protrusions 31 at the top. When the cabinet cover 20 is closed, the protrusions 31 abut against the top of the cabinet cover 30, and the side plate of the humidifying tank 30 without protrusions 31 forms a gas flow channel 32 with the cabinet cover 30.
[0057] The humidifying tank has an air inlet 11 and a water inlet 12, both of which are located outside the cabinet 10.
[0058] The gas flow channel 32 formed between the humidifying tank 30 and the heating glass 21 can make the gas distribution in the culture device more uniform and facilitate rapid replacement of the gas in the culture device.
[0059] The cover 20 is further provided with a protective shell 23 for protecting the heated glass 21, the protective shell 23 is detachably connected with the tank body 10, and the protective shell 23 is provided with a sealing ring 24 for sealing connection with the tank body 10.
[0060] The cells need to keep the stability of the environment during the culture process, so they are isolated from the external environment, and the structure of the sealing ring 24 can more effectively isolate the external environment.
[0061] Further comprising a humidity sensor 71 for detecting the humidity in the culture device in real time, and the humidity sensor 71 has a reminding function.
[0062] The humidity in the culture device directly affects the osmotic pressure of the culture solution, so when the humidity sensor 71 detects that the humidity value in the culture device starts to drop, the reminding function will start, reminding the operator to add water to the humidifying tank 30.
[0063] Further comprising a cover state detector 72 for detecting the sealing state between the cover 20 and the tank body 10.
[0064] After the cover state detector 72 detects that the tank body 10 and the cover 20 are in a sealed state, a large amount of gas blowing can be used to quickly replace and adjust the gas in the culture device, so that the gas concentration in the culture device is quickly restored.
Claims
1. A culture device for microscopy comprising a housing and a lid connected movably, characterized in that The warehouse body is provided with a placing area for placing culture dishes, and a humidifying groove is arranged around the placing area and detachably connected with the warehouse body. The placing area comprises a first heating assembly for heating the culture dishes, the humidifying groove comprises a second heating assembly for heating water in the humidifying groove, the first heating assembly and the second heating assembly are independent of each other, and the third heating assembly is arranged on the warehouse cover.
2. The culture device for microscopy according to claim 1, characterized by The placing area is provided with a placing ring for fixing the position of the culture dishes, a tray is arranged between the placing ring and the warehouse body bottom plate, the placing ring and the tray are detachably connected, and the tray is used for limiting the position of the placing ring.
3. The culture device for microscopy according to claim 2, characterized by The first heating assembly comprises a first heating sheet and a first uniform temperature sheet fixed with the warehouse body bottom plate, the first heating sheet and the first uniform temperature sheet are distributed on the entire warehouse body bottom plate, and the first uniform temperature sheet is used for making the temperature of the warehouse body bottom plate uniform.
4. The culture device for microscope according to claim 3, characterized by The warehouse body bottom plate is magnetically connected with the tray.
5. The culture device for microscopy according to claim 1, characterized by The second heating assembly comprises a second heating sheet arranged at the bottom of the humidifying groove and a heat preservation and insulation layer, and the heat preservation and insulation layer is arranged between the second heating sheet and the first heating assembly. The second heating assembly further comprises a third heating sheet and a second uniform temperature sheet arranged on the sidewall of the humidifying groove, and the second uniform temperature sheet is used for making the temperature of the sidewall of the humidifying groove uniform.
6. The culture device for microscopy according to claim 1, wherein The third heating assembly comprises a heating glass and a temperature sensor, and the heating glass and the temperature sensor are arranged on the warehouse cover.
7. The culture device for microscopy according to claim 6, characterized by The humidifying groove is open at the top, the sidewall of the humidifying groove is provided with a plurality of protrusions, when the warehouse cover is closed, the plurality of protrusions abut against the top of the warehouse cover, and a gas flow channel is formed between the top of the sidewall of the humidifying groove, which is not provided with protrusions, and the warehouse cover. The humidifying groove is provided with an air inlet and a water inlet, and the air inlet and the water inlet are located outside the warehouse body.
8. The culture device for microscopy according to claim 6, characterized by The warehouse cover is further provided with a protective shell for protecting the heating glass, the protective shell is detachably connected with the warehouse body, and the protective shell is provided with a sealing ring for sealing connection with the warehouse body.
9. The culture device for microscopy according to claim 1, characterized by The culture device further comprises a humidity sensor for detecting the humidity in the culture device in real time, and the humidity sensor has a reminding function.
10. The culture device for microscopy according to claim 1, characterized by The culture device further comprises a warehouse cover state detector for detecting the sealing state between the warehouse cover and the warehouse body.