Capacity-expandable stem cell culture solution extractor

Abstract

The utility model discloses an expandable stem cell culture solution extractor which comprises a stem cell culture solution extractor body, and the stem cell culture solution extractor body comprises an outer container and an inner cavity. A slidable piston is installed in the inner cavity, one end of the piston is connected with a pull rod, the inner cavity comprises an upper cavity body and a lower cavity body, and a gap communicated with the outer containing body is formed between the upper cavity body and the lower cavity body; the end, away from the pulling rod, of the inner cavity is connected with a suction nozzle, and the outer wall of the suction nozzle is connected with a connecting block. The outer container comprises a detachable bottom, and a mounting hole is formed in the bottom; and the inner cavity penetrates through the mounting hole and is connected with the mounting hole through a connecting block. According to the stem cell culture solution extractor, capacity expansion of the stem cell culture solution extractor body can be achieved, a large amount of stem cell culture solution can be transferred at a time, time is saved, the possibility that the stem cell culture solution is polluted in the transferring process is reduced, and the stem cell culture solution extractor body is simple in design, convenient to operate, capable of saving cost and convenient to use.

Description

TECHNICAL FIELD

[0001] The utility model belongs to stem cell culture technical field especially, it is a kind of stem cell culture solution extractor that can expand capacity. BACKGROUND

[0002] It is known that stem cell (stem cell, SC) "stem", translation from English "stem", means "stem" and "origin", the function of stem cell group is to control and maintain the regeneration of cell, generally speaking, there is the intermediate progenitor cell group called "directed progenitor cell" between stem cell and its terminal differentiated daughter cell, they have limited expansion capacity and limited differentiation potential, the function of these cell groups is to increase the number of differentiated cells produced after stem cell each time division, stem cell has the ability of self-renewal, but stem cell division is actually relatively asymmetric;Mesenchymal stem cells are derived from early developmental mesoderm and belong to multipotent stem cells, have multi-directional differentiation, and the adhesion growth characteristics of this type of cell culture have more restrictions, the existing culture technology is mostly used to culture mesenchymal stem cells in laboratory, and mass production is difficult.

[0003] In the experiment process, experimental liquid needs to be transferred many times, and experimental tools involve micropipette.The structure of the existing pipette is various, and the simple pipette is mostly dropper structure, which is convenient and fast for pipetting, but cannot accurately control the pipetting amount.In the process of doing precision experiment, the amount of stem cell solution needs to be accurately controlled, and often needs to be used with measuring device, and the pipetting process needs to be carried out in steps, so the efficiency of pipetting operation is not high. UTILITY MODEL CONTENT

[0004] The utility model aims at solving the existing technology in the experiment process that stem cell solution needs to be transferred many times, and experimental tools involve micropipette.The structure of the existing pipette is various, and the simple pipette is mostly dropper structure, which is convenient and fast for pipetting, but cannot accurately control the pipetting amount.In the process of doing precision experiment, the amount of stem cell solution needs to be accurately controlled, and often needs to be used with measuring device, and the pipetting process needs to be carried out in steps, so the efficiency of pipetting operation is not high.For the above problems, it is necessary to invent a stem cell culture solution extractor that can expand capacity.

[0005] In order to realize the above object, the utility model adopts the following technical scheme:

[0006] A stem cell culture solution extractor that can expand capacity, including stem cell culture solution extractor body, the stem cell culture solution extractor body includes outer container and inner cavity body;

[0007] A slidable piston is installed in the inner cavity, one end of the piston is connected with a pulling rod, the inner cavity comprises an upper cavity and a lower cavity, a gap is arranged between the upper cavity and the lower cavity and is communicated with the outer container;

[0008] A suction nozzle is connected to one end of the inner cavity away from the pulling rod, and a connecting block is connected to the outer wall of the suction nozzle;

[0009] The outer container comprises a detachable bottom, and the bottom is provided with a mounting hole;

[0010] The inner cavity is connected with the mounting hole through the connecting block.

[0011] Further, the outer container and the inner cavity are made of transparent material.

[0012] Further, threads are arranged on the outer wall of the connecting block and in the mounting hole; and the connecting block is connected with the mounting hole through threads.

[0013] Further, the outer container is connected with the bottom through threads.

[0014] Further, a pulling handle is connected to one end of the pulling rod away from the piston.

[0015] Compared with the prior art, the beneficial effects of the present application are:

[0016] The present application relates to a stem cell culture solution extractor, which comprises a stem cell culture solution extractor body, wherein the stem cell culture solution extractor body comprises an outer container and an inner cavity; a slidable piston is installed in the inner cavity, one end of the piston is connected with a pulling rod, the inner cavity comprises an upper cavity and a lower cavity, a gap is arranged between the upper cavity and the lower cavity and is communicated with the outer container; a suction nozzle is connected to one end of the inner cavity away from the pulling rod, and a connecting block is connected to the outer wall of the suction nozzle; the outer container comprises a detachable bottom, and the bottom is provided with a mounting hole; and the inner cavity is connected with the mounting hole through the connecting block.

[0017] The stem cell culture solution extractor body can be expanded, a large amount of stem cell culture solution can be transferred at one time, time is saved, the possibility of contamination of the stem cell culture solution in the transfer process is reduced, the stem cell culture solution extractor body is simple in design, easy to operate, cost is saved, and convenience is provided. BRIEF DESCRIPTION OF DRAWINGS

[0018] Fig. 1 It is a whole structure schematic view of the present application;

[0019] Fig. 2 It is an outer container, mounting hole and bottom structure schematic view of the present application;

[0020] Fig. 3The utility model discloses a structure schematic diagram of inner cavity, suction nozzle and connecting block.

[0021] Marked as in the drawing:

[0022] 1-outer container, 2-inner cavity, 3-piston, 4-pull rod, 5-gap, 6-suction nozzle, 7-connecting block, 8-mounting hole;

[0023] 11-bottom, 12-thread, 13-handle;

[0024] 21-upper cavity, 22-lower cavity. DETAILED DESCRIPTION

[0025] In order to make the technical problems solved by the utility model, the technical scheme adopted and the technical effects reached more clear, the technical scheme of the utility model will be further explained below by specific implementation manners in connection with the drawings.

[0026] In the description of the utility model, it is understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model.

[0027] In addition, the features defined as "first" and "second" can explicitly or implicitly include one or more features for distinguishing the described features, and there is no order or difference. In the description of the utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0028] In the description of the utility model, it should be explained that, unless otherwise specified and limited, the terms "mounting", "connection" and "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected, it can be mechanically connected, or it can be electrically connected, it can be directly connected, or indirectly connected through an intermediate medium, or it can be the communication between two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0029] Reference Figs. 1-3 A stem cell culture solution extractor capable of expanding capacity includes a stem cell culture solution extractor body, and the stem cell culture solution extractor body includes an outer container 1 and an inner cavity 2.

[0030] A slidable piston 3 is installed in the inner cavity 2, and one end of the piston 3 is connected with a pulling rod 4. The inner cavity 2 comprises an upper cavity 21 and a lower cavity 22, and a gap 5 is arranged between the upper cavity 21 and the lower cavity 22, which is communicated with the outer container 1;

[0031] A suction nozzle 6 is connected with one end of the inner cavity 2 away from the pulling rod 4, and an outer wall of the suction nozzle 6 is connected with a connecting block 7;

[0032] The outer container 1 comprises a detachable bottom 11, and the bottom 11 is provided with a mounting hole 8.

[0033] The inner cavity 2 is connected with the mounting hole 8 through the connecting block 7.

[0034] Further preferably, the outer container 1 and the inner cavity 2 are made of transparent material.

[0035] Further preferably, a screw thread 12 is arranged on an outer wall of the connecting block 7.

[0036] Further preferably, a screw thread 12 is arranged in the mounting hole 8.

[0037] Further preferably, the connecting block 7 is connected with the mounting hole 8 through the screw thread 12.

[0038] Further preferably, the outer container 1 is connected with the bottom 11 through the screw thread 12.

[0039] Further preferably, a pulling handle 13 is connected with one end of the pulling rod 4 away from the piston 3.

[0040] The utility model discloses a basic working principle: when working, the piston 3 is located inside lower cavity 22, pushes down and pulls pull rod 4, pull rod 4 pushes down piston 3 until can not push down again, air in the lower cavity 22 is discharged in this way, then inserts suction nozzle 6 into stem cell culture solution, pulls up pull rod 4, pull rod 4 pulls piston 3, under the condition that the pressure difference between inside and outside, stem cell culture solution will enter inside inner cavity 2 along suction nozzle 6, continues to pull piston 3 until piston 3 enters upper cavity 21, under the condition that the pressure difference between inside and outside, stem cell culture solution continues to flow upwards along inside inner cavity 2, then enters outer container 1 along gap 5, until stem cell culture solution stops flowing into outer container 1, then air in outer container 1 enters inner cavity 2, pushes down and pulls pull rod 4, pull rod 4 pushes down piston 3 until can not push down again, air in the lower cavity 22 is discharged in this way, then inserts suction nozzle 6 into stem cell culture solution, pulls up pull rod 4, pull rod 4 pulls piston 3, so repeatedly several times until pulling up piston 3, stem cell culture solution does not flow into outer container 1 and inner cavity 2 along suction nozzle 6 any more, or pulls up piston 3, the amount of stem cell culture solution flowing into outer container 1 and inner cavity 2 along suction nozzle 6 does not increase any more, stops pushing down and pulling pull rod 4.

[0041] The utility model discloses a basic working principle: when working, the piston 3 is located inside lower cavity 22, pushes down and pulls pull rod 4, pull rod 4 pulls piston 3 until can not push down again, air in the lower cavity 22 is discharged in this way, then inserts suction nozzle 6 into stem cell culture solution, pulls up pull rod 4, pull rod 4 pulls piston 3, under the condition that the pressure difference between inside and outside, stem cell culture solution will enter inside inner cavity 2 along suction nozzle 6, continues to pull piston 3 until piston 3 enters upper cavity 21, under the condition that the pressure difference between inside and outside, stem cell culture solution continues to flow upwards along inside inner cavity 2, then enters outer container 1 along gap 5, until stem cell culture solution stops flowing into outer container 1, then air in outer container 1 enters inner cavity 2, pushes down and pulls pull rod 4, pull rod 4 pushes down piston 3 until can not push down again, air in the lower cavity 22 is discharged in this way, then inserts suction nozzle 6 into stem cell culture solution, pulls up pull rod 4, pull rod 4 pulls piston 3, so repeatedly several times until pulling up piston 3, stem cell culture solution does not flow into outer container 1 and inner cavity 2 along suction nozzle 6 any more, or pulls up piston 3, the amount of stem cell culture solution flowing into outer container 1 and inner cavity 2 along suction nozzle 6 does not increase any more, stops pushing down and pulling pull rod 4.

[0042] The utility model discloses a basic working principle: when working, the piston 3 is located inside lower cavity 22, pushes down and pulls pull rod 4, pull rod 4 pulls piston 3 until can not push down again, air in the lower cavity 22 is discharged in this way, then inserts suction nozzle 6 into stem cell culture solution, pulls up pull rod 4, pull rod 4 pulls piston 3, under the condition that the pressure difference between inside and outside, stem cell culture solution will enter inside inner cavity 2 along suction nozzle 6, continues to pull piston 3 until piston 3 enters upper cavity 21, under the condition that the pressure difference between inside and outside, stem cell culture solution continues to flow upwards along inside inner cavity 2, then enters outer container 1 along gap 5, until stem cell culture solution stops flowing into outer container 1, then air in outer container 1 enters inner cavity 2, pushes down and pulls pull rod 4, pull rod 4 pushes down piston 3 until can not push down again, air in the lower cavity 22 is discharged in this way, then inserts suction nozzle 6 into stem cell culture solution, pulls up pull rod 4, pull rod 4 pulls piston 3, so repeatedly several times until pulling up piston 3, stem cell culture solution does not flow into outer container 1 and inner cavity 2 along suction nozzle 6 any more, or pulls up piston 3, the amount of stem cell culture solution flowing into outer container 1 and inner cavity 2 along suction nozzle 6 does not increase any more, stops pushing down and pulling pull rod 4.

[0043] The inner cavity 2 is connected with a suction nozzle 6 at one end away from the pulling rod 4, and the outer wall of the suction nozzle 6 is connected with a connecting block 7;

[0044] The outer container 1 comprises a detachable bottom 11, and the bottom 11 is provided with a mounting hole 8.

[0045] The inner cavity 2 is screwed together with the mounting hole 8 through the connecting block 7 and the screw thread 12 arranged on the inner wall of the mounting hole 8.

[0046] The outer container 1 is connected with the bottom 11 through the screw thread 12.

[0047] The pulling rod 4 is connected with a pulling handle 13 at one end away from the piston 3, and the pulling rod 4 is pulled through the handle 13 and drives the piston 3 to move up and down, which is more labor-saving.

[0048] The stem cell culture solution extractor body comprises an outer container 1 and an inner cavity 2; the inner cavity 2 is installed with a slidable piston 3, one end of the piston 3 is connected with a pulling rod 4, the inner cavity 2 comprises an upper cavity 21 and a lower cavity 22, and the upper cavity 21 and the lower cavity 22 are separated by a gap 5 in communication with the outer container 1; the inner cavity 2 is connected with a suction nozzle 6 at one end away from the pulling rod 4, and the outer wall of the suction nozzle 6 is connected with a connecting block 7; the outer container 1 comprises a detachable bottom 11, and the bottom 11 is provided with a mounting hole 8; the inner cavity 2 is connected with the mounting hole 8 through the connecting block 7.

[0049] The stem cell culture solution extractor body can be expanded, a large amount of stem cell culture solution can be transferred at one time, time is saved, the possibility of contamination of the stem cell culture solution in the transfer process is reduced, the stem cell culture solution extractor body is simple in design, convenient to operate, cost-saving, and convenient.

[0050] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any skilled person in the art can make equivalent replacement or change according to the technical scheme and the inventive concept of the present application within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.

[0051] In the description of the present specification, the description referring to the terms "some embodiments", "other embodiments", and the like mean that the particular feature, structure, material or characteristic being described is included in at least one embodiment or example of the present application. The illustrative appearances of the above-mentioned terms in various places in the specification are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics can be combined in any suitable manner in one or more embodiments or examples.

Claims

1. An expandable stem cell culture medium extractor, characterized in that: The device includes a stem cell culture medium extractor body, which includes an outer body (1) and an inner cavity (2). A sliding piston (3) is installed inside the inner cavity (2). One end of the piston (3) is connected to a pull rod (4). The inner cavity (2) includes an upper cavity (21) and a lower cavity (22). There is a gap (5) between the upper cavity (21) and the lower cavity (22) that communicates with the outer cavity (1). The end of the inner cavity (2) away from the pull rod (4) is connected to a suction nozzle (6), and a connecting block (7) is connected to the outer wall of the suction nozzle (6). The outer container (1) includes a removable bottom (11) with mounting holes (8). The inner cavity (2) passes through the mounting hole (8) and is connected to the mounting hole (8) via the connecting block (7).

2. The expandable stem cell culture medium extractor according to claim 1, characterized in that: Both the outer container (1) and the inner cavity (2) are made of transparent material.

3. The expandable stem cell culture medium extractor according to claim 1, characterized in that: The connecting block (7) is provided with threads (12) on its outer wall and in its mounting hole (8); the connecting block (7) is connected to the mounting hole (8) by the threads (12).

4. The expandable stem cell culture medium extractor according to claim 1, characterized in that: The outer body (1) is connected to the bottom (11) by a thread (12).

5. The expandable stem cell culture medium extractor according to claim 1, characterized in that: The end of the pull rod (4) away from the piston (3) is connected to a pull handle (13).

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