Molecular extraction mechanism and molecular diagnosis pretreatment equipment

Abstract

The utility model relates to the technical field of gene detection, and particularly discloses a molecular extraction mechanism and molecular diagnosis pretreatment equipment, in the molecular extraction mechanism, a rack is provided with an extraction station capable of containing a test tube; the flapping driving assembly is arranged on the rack; a flapping piece of the flapping assembly is provided with a containing groove which extends in the Z direction and is provided with an upward opening. The flapping piece is arranged at the output end of the flapping driving assembly and can synchronously move along with the output end of the flapping driving assembly, so that the flapping part of the flapping piece flaps a sample in the test tube; the extraction driving assembly is arranged on the rack; the extraction assembly comprises a magnetic bar, and the magnetic bar is in transmission connection with the output end of the extraction driving assembly and can move into the containing groove or move out of the containing groove along with the output end of the extraction driving assembly in the Z direction. According to the arrangement, the nucleic acid extraction efficiency is improved, the possibility of being polluted is also reduced, meanwhile, the magnetic bar is prevented from being cleaned, waste generated by cleaning is reduced, and the cost is saved.

Description

TECHNICAL FIELD

[0001] The utility model relates to gene detection technical field especially relates to a molecular extraction mechanism and molecular diagnosis pretreatment equipment. BACKGROUND

[0002] In the process of molecular diagnostic detection, a series of processing need to be done to the collected sample, including sample oscillation mixing, cap opening and liquid separation, adding various reaction reagents and enzymes, then pipetting to the corresponding consumables, and transferring to the specific equipment for nucleic acid extraction; wherein, the nucleic acid extraction process needs to move the consumables with mixed liquid to the beating station first, then beat the mixed liquid effectively through the beating mechanism, so that the magnetic beads and nucleic acid are in full contact; then move the consumables with mixed liquid to the extraction station, extend the magnetic rod into the consumables to adsorb the magnetic beads with nucleic acid, and take out the magnetic beads in the process of extending the magnetic rod, finally separate the magnetic beads and the magnetic rod for subsequent operation.

[0003] The transfer process between multiple stations wastes time, resulting in low nucleic acid extraction efficiency, and the transfer process is easy to be contaminated, reducing the quality of nucleic acid extraction.

[0004] Therefore, it is urgent to study a molecular extraction mechanism and molecular diagnostic pretreatment equipment to solve the above problems. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a molecular extraction mechanism and molecular diagnostic pretreatment equipment to solve the problem of low extraction efficiency and easy contamination caused by the need for multiple station transfer in the nucleic acid extraction process in the prior art.

[0006] To achieve the above purpose, the utility model adopts the following technical scheme:

[0007] A molecular extraction mechanism, comprising:

[0008] A rack with an extraction station capable of holding a test tube;

[0009] A beating drive assembly provided in the rack;

[0010] A beating assembly, the beating part of the beating assembly has a containing groove extending in the Z direction and opening upward; the beating part is provided on the output end of the beating drive assembly and can move synchronously with the output end of the beating drive assembly to beat the sample in the test tube;

[0011] An extraction drive assembly provided in the rack;

[0012] An extraction assembly comprising a magnetic rod, which is in transmission connection with the output end of the extraction driving assembly and can move along with the output end of the extraction driving assembly in the Z direction to move into or out of the accommodating groove.

[0013] As an optional technical scheme of the molecular extraction mechanism, the beating assembly further comprises a beating support, which is arranged at the output end of the beating driving assembly, and the beating piece is detachably connected with the beating support.

[0014] As an optional technical scheme of the molecular extraction mechanism, the beating support has an insertion slot extending in the Y direction, and the beating piece has a beating body and an insertion block arranged on the beating body, and the insertion block is inserted into the insertion slot.

[0015] As an optional technical scheme of the molecular extraction mechanism, the beating support has two insertion slots, which are oppositely arranged, and one end of the insertion slot is not closed to form an insertion port, and the beating piece has two insertion blocks, which are arranged on both sides of the beating body in the X direction; the two insertion blocks can be inserted into the two insertion slots from the two insertion ports.

[0016] As an optional technical scheme of the molecular extraction mechanism, the lower surface of the beating body extends downward to form a strip-shaped protrusion to form a beating part, and the accommodating groove is arranged concentrically in the strip-shaped protrusion.

[0017] As an optional technical scheme of the molecular extraction mechanism, the lower surface of the beating body is arranged with a plurality of beating parts arranged in a matrix, and each of the beating parts is arranged with an accommodating groove.

[0018] As an optional technical scheme of the molecular extraction mechanism, the beating driving assembly comprises:

[0019] A beating driving piece arranged at the rack;

[0020] A beating driving piece arranged at the rack;

[0021] A beating driven wheel rotatably arranged on the rack and spaced apart from the beating driving wheel in the Z direction;

[0022] A beating transmission piece arranged between the beating driving wheel and the beating driven wheel and in transmission connection with the beating piece.

[0023] As an optional technical scheme of the molecular extraction mechanism, the extraction driving assembly comprises:

[0024] An extraction driving piece arranged at the rack;

[0025] An extraction driving wheel is arranged at the output end of the extraction driving member;

[0026] An extraction driven wheel is rotatably arranged on the frame and is arranged in the Z direction apart from the extraction driving wheel;

[0027] An extraction transmission member is arranged between the extraction driving wheel and the extraction driven wheel and is in transmission connection with the extraction assembly.

[0028] As an optional technical solution of the molecular extraction mechanism, the molecular extraction mechanism further comprises:

[0029] A sliding rail is arranged on the base and extends in the Z direction;

[0030] A first sliding block is in sliding cooperation with the sliding rail and is connected with the beating member;

[0031] A second sliding block is in sliding cooperation with the sliding rail and is connected with the extraction assembly.

[0032] The molecular diagnostic pretreatment equipment comprises the molecular extraction mechanism according to any one of the above technical solutions.

[0033] The molecular extraction mechanism and the molecular diagnostic pretreatment equipment have at least the following beneficial effects:

[0034] The molecular extraction mechanism and the molecular diagnostic pretreatment equipment have at least the following beneficial effects: BRIEF DESCRIPTION OF DRAWINGS

[0035] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the description of the embodiments of the present application will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present application and the drawings without creative labor.

[0036] Fig. 1This is a schematic diagram of the molecular extraction mechanism in an embodiment of the present invention;

[0037] Fig. 2 This is a partial cross-sectional view of the molecular extraction mechanism in an embodiment of this utility model;

[0038] Fig. 3 This is a schematic diagram of the structure of the tapping drive assembly and the tapping assembly in the embodiments of this utility model;

[0039] Fig. 4 This is a schematic diagram of the extraction driving component and the extraction component in the embodiments of this utility model.

[0040] In the picture:

[0041] 100. Frame; 110. Slide rail; 121. First slider; 122. Second slider;

[0042] 200. Beating drive assembly; 210. Beating drive component; 220. Beating drive wheel; 230. Beating driven wheel; 240. Beating transmission component;

[0043] 300, Tapping assembly; 310, Tapping component; 311, Tapping body; 312, Tapping section; 3121, Receiving groove; 313, Insertion block; 320, Tapping bracket; 321, Insertion groove;

[0044] 400. Extract the drive assembly; 410. Extract the drive component; 420. Extract the drive wheel; 430. Extract the driven wheel; 440. Extract the transmission component;

[0045] 500. Extraction component; 510. Magnetic rod; 520. Extraction base. Detailed Implementation

[0046] Before explaining any implementation of this application in detail, it should be understood that this application is not limited to its application to the structural details and component arrangements set forth in the following description or shown in the above drawings.

[0047] In this application, the terms "comprising," "including," "having," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0048] In the present application, the term "and / or", is a description of an associated relationship with associated objects, which means that there can be three kinds of relationships. For example, A and / or B, can represent: the existence of A alone, the existence of A and B together, and the existence of B alone. In addition, the character " / " in the present application generally indicates that the front and rear associated objects are in a "and / or" relationship.

[0049] In the present application, the terms "connected", "combined", "coupled", "mounted" can be direct connection, combination, coupling or mounting, or indirect connection, combination, coupling or mounting. Among them, for example, direct connection refers to the connection of two parts or components without the need for an intermediate part, while indirect connection refers to the connection of two parts or components with at least one intermediate part. In addition, "connection" and "coupling" are not limited to physical or mechanical connection or coupling, and can include electrical connection or coupling.

[0050] In the present application, those of ordinary skill in the art will understand that the relative terms used in connection with quantities or conditions (for example, "about", "approximately", "substantially" and the like) include the values described and have the meaning indicated by the context. For example, the relative terms at least include the degree of error related to the measurement of a specific value, the tolerance caused by manufacturing, assembly, use, etc. related to a specific value. Such terms should also be considered to disclose the range defined by the absolute values of the two endpoints. The relative term can refer to a certain percentage (for example, 1%, 5%, 10% or more) of the indicated value. The numerical value without the relative term should also be disclosed as a specific value with a tolerance. In addition, "substantially" when expressing the relative angular positional relationship (for example, substantially parallel, substantially perpendicular), can refer to a certain number of degrees (for example, 1 degree, 5 degrees, 10 degrees or more) added or subtracted from the indicated angle.

[0051] In the present application, those of ordinary skill in the art will understand that the functions performed by the components can be performed by one component, multiple components, one part, or multiple parts. Similarly, the functions performed by the parts can also be performed by one part, one component, or multiple parts in combination.

[0052] In the present application, the terms "upper", "lower", "left", "right", "front", "back" and the like are described in the orientation and positional relationship shown in the drawings, and should not be understood as a limitation on the embodiments of the present application. In addition, it is also understood in the context that when referring to an element connected to another element "on" or "under", it can be directly connected to another element "on" or "under" or indirectly connected to another element "on" or "under" through an intermediate element. It should also be understood that the terms "upper", "lower", "left", "right", "front", "back" and the like not only represent the positive direction, but also can be understood as the side direction. For example, the lower side can include the directly below, left below, right below, front below and back below, etc.

[0053] As shown in Figs. 1 to 4 The present embodiment provides a molecular extraction mechanism, which comprises a rack 100, a beating driving assembly 200, a beating assembly 300, an extraction driving assembly 400 and an extraction assembly 500. The rack 100 has an extraction station capable of containing a test tube; the beating driving assembly 200 is arranged on the rack 100; the beating piece 310 of the beating assembly 300 has a containing groove 3121 extending along the Z direction and opening upward; the beating piece 310 is arranged on the output end of the beating driving assembly 200 and can move synchronously with the output end of the beating driving assembly 200 to make the beating part 312 of the beating piece 310 beat the sample in the test tube; the extraction driving assembly 400 is arranged on the rack 100; the extraction assembly 500 comprises a magnetic rod 510, which is in transmission connection with the output end of the extraction driving assembly 400 and can move along the Z direction to move into or out of the containing groove 3121.

[0054] With the above arrangement, the beating driving assembly 200 can drive the beating assembly 300 to beat the sample in the test tube, so that the magnetic beads and the nucleic acid are in sufficient contact to adsorb as much nucleic acid as possible; then the extraction driving assembly 400 drives the extraction assembly 500 to make the magnetic beads with nucleic acid be adsorbed, thereby completing the transfer of the magnetic beads, and the whole process does not need to transfer the position of the test tube, thereby improving the nucleic acid extraction efficiency and reducing the possibility of contamination. In addition, the magnetic rod 510 extends into the containing groove 3121 of the beating piece 310, so that the magnetic beads with nucleic acid are adsorbed on the outer surface of the beating piece 310; subsequent only needs to clean the beating piece 310, avoids cleaning the magnetic rod 510, reduces the waste caused by cleaning, and saves the cost.

[0055] In some embodiments, the beating assembly 300 further comprises a beating bracket 320 arranged at the output end of the beating driving assembly 200, and the beating piece 310 is detachably connected with the beating bracket 320. This arrangement enables the beating piece 310 to be replaced in time after being damaged or replaced with different models when facing different samples.

[0056] Regarding the specific structure of the beating piece 310, in some embodiments, the lower surface of the beating body 311 extends downward to form a beating part 312, and a containing groove 3121 is arranged in the strip-shaped protrusion and concentrically arranged with the strip-shaped protrusion. The strip-shaped protrusion and the beating body 311 are integrally formed. This arrangement can reduce the material used by the beating part 312 and reduce costs, and also enables the thickness of the side wall of the containing groove 3121 to be uniform. During the beating process, the beating part 312 reciprocates up and down in the test tube, so that the solution in the test tube is agitated.

[0057] To facilitate the connection of the beating piece 310 and the beating bracket 320, in some embodiments, the beating bracket 320 has a plug-in groove 321 extending along the Y direction, the beating piece 310 has a beating body 311 and a plug-in block 313 arranged on the beating body 311, and the plug-in block 313 is plugged into the plug-in groove 321. The Y direction is perpendicular to the Z direction. The plug-in mode is convenient to operate, and the plug-in direction is perpendicular to the beating direction, which avoids the beating piece 310 and the beating bracket 320 from being separated during the beating process, thereby improving the connection stability. In other embodiments, the beating piece 310 and the beating bracket 320 can also be screwed or clamped.

[0058] Further, the beating bracket 320 has two plug-in grooves 321 arranged oppositely, one end of the plug-in groove 321 is not closed to form a plug-in port, and the beating piece 310 has two plug-in blocks 313 arranged on both sides of the beating body 311 along the X direction; the two plug-in blocks 313 can be inserted into the two corresponding plug-in grooves 321 from the two plug-in ports. The cooperation of the two plug-in blocks 313 and the two plug-in grooves 321 makes the force on the beating piece 310 more stable, further improving the stability of the connection between the beating piece 310 and the beating bracket 320. The X direction is perpendicular to the Z direction and the Y direction.

[0059] To improve the nucleic acid extraction efficiency, in some embodiments, the lower surface of the beating body 311 is arranged with a plurality of beating parts 312 arranged in a matrix, and each beating part 312 is provided with a containing groove 3121. This arrangement enables the samples in several test tubes to be beaten at a time, thereby increasing the number of samples beaten at a time.

[0060] In the embodiment, the beating driving assembly 200 comprises a beating driving member 210, a beating driving wheel 220, a beating driven wheel 230 and a beating transmission member 240. The beating driving member 210 is arranged on the rack 100. The beating driving wheel 220 is arranged at the output end of the beating driving member 210. The beating driven wheel 230 is rotatably arranged on the rack 100 and is arranged in the Z direction at a distance from the beating driving wheel 220. The beating transmission member 240 is arranged around the beating driving wheel 220 and the beating driven wheel 230 and is in transmission connection with the beating member 310. This arrangement makes the beating driving member 210 a rotary driving member such as a motor. In use, the rotary driving member is alternately rotated in the positive and negative directions, so as to realize the reciprocating movement of the beating member 310 in the Z direction.

[0061] The beating transmission member 240 comprises a transmission belt. Further, the beating driving wheel 220 and the beating driven wheel 230 are both toothed wheels, and the beating transmission member 240 is a synchronous belt, so as to improve the precision of the single stroke distance in the reciprocating movement of the beating member 310.

[0062] In some embodiments, the extraction driving assembly 400 comprises an extraction driving member 410, an extraction driving wheel 420, an extraction driven wheel 430 and an extraction transmission member 440. The extraction driving member 410 is arranged on the rack 100. The extraction driving wheel 420 is arranged at the output end of the extraction driving member 410. The extraction driven wheel 430 is rotatably arranged on the rack 100 and is arranged in the Z direction at a distance from the extraction driving wheel 420. The extraction transmission member 440 is arranged around the extraction driving wheel 420 and the extraction driven wheel 430 and is in transmission connection with the extraction assembly 500. This arrangement makes the extraction driving member 410 a rotary driving member such as a motor. In use, the rotary driving member is alternately rotated in the positive and negative directions, so as to realize the reciprocating movement of the extraction assembly 500 in the Z direction.

[0063] The extraction transmission member 440 is an extraction transmission belt. Further, the extraction driving wheel 420 and the extraction driven wheel 430 are both toothed wheels, and the extraction transmission member 440 is a synchronous belt, so as to improve the precision of the single stroke distance in the reciprocating movement of the extraction assembly 500. The extraction assembly 500 further comprises an extraction base 520, and the magnetic rod 510 is arranged on the extraction base 520. The extraction base 520 is connected with the extraction transmission member 440.

[0064] In order to improve the smoothness of the movement of the beating member 310 and the extraction assembly 500, in some embodiments, the molecular extraction mechanism further comprises a sliding rail 110, a first sliding block 121 and a second sliding block 122. The sliding rail 110 is arranged on the base and extends in the Z direction. The first sliding block 121 is in sliding connection with the sliding rail 110 and is connected with the beating member 310. The second sliding block 122 is in sliding connection with the sliding rail 110 and is connected with the extraction assembly 500. The second sliding block 122 is located above the first sliding block 121, so as to facilitate the control of the extraction assembly 500 and the beating member 310 and avoid interference between them.

[0065] The embodiment also provides a molecular diagnosis pretreatment device comprising the molecular extraction mechanism in the above embodiment.

[0066] Obviously, the above embodiments of the present application are only examples for clearly illustrating the present application, and are not intended to limit the implementation modes of the present application. For those skilled in the art, various obvious changes, re-adjustments and substitutions can be made without departing from the protection scope of the present application. Here, it is not necessary and impossible to enumerate all the implementation modes. Any modification, equivalent substitution and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application claim.

Claims

1. A molecular extraction mechanism, characterized in that, include: The frame (100) has an extraction station for holding test tubes; A tapping drive assembly (200) is disposed on the frame (100); A tapping assembly (300) has a tapping member (310) having a receiving groove (3121) extending along the Z direction and opening upward; the tapping member (310) is disposed at the output end of the tapping drive assembly (200) and can move synchronously with the output end of the tapping drive assembly (200) so that the tapping part (312) of the tapping member (310) taps the sample in the test tube; Extract the drive assembly (400) and place it on the rack (100); Extraction component (500) includes a magnetic rod (510) which is connected to the output end of the extraction drive component (400) and can move along the Z direction to move into or out of the receiving groove (3121).

2. The molecular extraction mechanism according to claim 1, characterized in that, The slapping assembly (300) further includes a slapping bracket (320), which is located at the output end of the slapping drive assembly (200). The slapping member (310) is detachably connected to the slapping bracket (320).

3. The molecular extraction mechanism according to claim 2, characterized in that, The slapping bracket (320) has a insertion groove (321) extending along the Y direction, and the slapping member (310) has a slapping body (311) and an insertion block (313) disposed on the slapping body (311), the insertion block (313) being inserted into the insertion groove (321).

4. The molecular extraction mechanism according to claim 3, characterized in that, The tapping bracket (320) has two insertion slots (321) arranged opposite to each other. One end of each insertion slot (321) is not closed to form an insertion port. The tapping component (310) has two insertion blocks (313). Along the X direction, the two insertion blocks (313) are respectively disposed on both sides of the tapping body (311). The two insertion blocks (313) can be inserted into the two insertion slots (321) from the two insertion ports.

5. The molecular extraction mechanism according to claim 3, characterized in that, The lower surface of the striking body (311) extends downward into a strip-shaped protrusion to form a striking part (312). The receiving groove (3121) is located in the strip-shaped protrusion and is arranged concentrically with the strip-shaped protrusion.

6. The molecular extraction mechanism according to claim 5, characterized in that, The lower surface of the striking body (311) is provided with a plurality of striking parts (312) arranged in a matrix, and each striking part (312) is provided with a receiving groove (3121).

7. The molecular extraction mechanism according to any one of claims 1-6, characterized in that, The tapping drive assembly (200) includes: A striking drive unit (210) is provided on the frame (100); A tapping drive wheel (220) is located at the output end of the tapping drive member (210); The driven wheel (230) is rotatably mounted on the frame (100) and is spaced apart from the driving wheel (220) along the Z direction. A slapping transmission component (240) is wound around the slapping drive wheel (220) and the slapping driven wheel (230), and is connected to the slapping component (310) in a transmission manner.

8. The molecular extraction mechanism according to any one of claims 1-6, characterized in that, The extraction driving component (400) includes: Extract the drive unit (410) and place it on the frame (100); Extraction drive wheel (420) is located at the output end of extraction drive unit (410); The driven wheel (430) is rotatably mounted on the frame (100) and is spaced apart from the driving wheel (420) along the Z direction. Extraction transmission component (440) is wound around the extraction drive wheel (420) and the extraction driven wheel (430), and is connected to the extraction assembly (500) in a transmission manner.

9. The molecular extraction mechanism according to any one of claims 1-6, characterized in that, The molecular extraction mechanism also includes: A slide rail (110) is provided on the base and extends along the Z direction; The first slider (121) is slidably engaged with the slide rail (110) and connected to the striking member (310); The second slider (122) is slidably engaged with the slide rail (110) and connected to the extraction component (500).

10. Molecular diagnostic pretreatment equipment, characterized in that, Includes the molecular extraction mechanism as described in any one of claims 1-9.

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