Protein ultrafiltration concentration centrifuge tube for laboratory

Abstract

The utility model discloses a protein ultrafiltration concentration centrifuge tube for a laboratory, which comprises a centrifuge tube body with an upper opening structure. The cover body is connected to the upper part of the centrifugal tube body; the ultrafiltration sleeve assembly is arranged in the centrifugal tube body, a liquid outlet is formed in the bottom of the ultrafiltration sleeve assembly, a threaded cover is connected to the liquid outlet, and a sample subjected to ultrafiltration through the ultrafiltration sleeve assembly flows out through the liquid outlet and is collected through the threaded cover. The protein ultrafiltration concentration centrifuge tube for the laboratory is simple in structure, the supporting piece is of a conical fold porous structure, the cellulose membrane is attached to the surface of the supporting piece, the area of the ultrafiltration membrane can be increased, the protein ultrafiltration concentration centrifuge tube can be used for ultrafiltration concentration of a protein sample in the laboratory, the volume of treated liquid is small, the ultrafiltration efficiency is high, the sample is convenient to collect, and loss is small.

Description

Technical Field

[0001] This utility model relates to the field of biomedical equipment technology, and in particular to a laboratory protein ultrafiltration concentration centrifuge tube. Background Technology

[0002] Ultrafiltration is a highly efficient and commonly used protein concentration technique. It utilizes ultrafiltration membranes with specially designed pore sizes to separate and concentrate proteins through centrifugal force. The pore size of this semi-permeable membrane can be selected according to requirements to effectively separate molecules of different sizes. When a solution is subjected to centrifugal force, driven by tangential flow, the membrane separation process occurs based on the principle of molecular sieving. Molecules smaller than the membrane pore size (such as water molecules and small solute molecules) can pass through the membrane, while molecules larger than the pore size (such as proteins) are retained on one side of the membrane. In this way, proteins are concentrated, while small solute molecules are removed, achieving the purpose of separation, purification, and concentration. The pore size of ultrafiltration membranes is typically between 3 and 300 nm. By selecting ultrafiltration membranes with different pore sizes, substances with relative molecular masses ranging from hundreds to hundreds of thousands can be separated. The operating pressure of ultrafiltration membranes is generally 0.1–0.5 MPa. Ultrafiltration technology has been widely used in food, pharmaceutical, ultrapure water preparation, and biotechnology fields.

[0003] Industrial-grade ultrafiltration concentration already has fully automated large-scale equipment. However, in biomedical research laboratories, such as for purifying antibody samples and recombinant antigen samples, the sample concentration is low, the processing volume is small, and the samples are precious and difficult to pass through the filtration membrane. Currently, there are commercially available ultrafiltration concentration centrifuge tubes, but they have disadvantages such as small membrane contact area during processing, difficulty in ultrafiltration, low efficiency, and difficulty in collecting small samples.

[0004] Based on the above-mentioned technical problems, those skilled in the art urgently need to develop a protein ultrafiltration concentration centrifuge tube to solve the problems of viscous liquids that are difficult to filter in laboratory protein ultrafiltration, and small amounts of concentrated target protein that are difficult to collect. This would facilitate the concentration of small amounts of protein samples in the laboratory, enabling rapid ultrafiltration and improving sample recovery rate. Utility Model Content

[0005] The purpose of this invention is to provide a laboratory protein ultrafiltration centrifuge tube with a simple structure that facilitates small-scale ultrafiltration concentration of protein samples in the laboratory, enabling rapid ultrafiltration and improved sample recovery.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] This invention relates to a laboratory protein ultrafiltration concentration centrifuge tube, which comprises:

[0008] The centrifuge tube body has an open top structure; and

[0009] A cover connected to the upper part of the centrifuge tube body;

[0010] The centrifuge tube also includes:

[0011] The ultrafiltration sleeve assembly located in the centrifuge tube body has a liquid outlet at the bottom and a threaded cap connected to the liquid outlet. The sample after ultrafiltration by the ultrafiltration sleeve assembly flows out through the liquid outlet and is collected through the threaded cap.

[0012] Preferably, the centrifuge tube body is a milliliter polypropylene pointed-bottom centrifuge tube, and the opening of the centrifuge tube body is provided with external threads on the outside.

[0013] The cover is made of polyethylene material and has internal threads that are threadedly connected to the centrifuge tube body.

[0014] Preferably, the upper part of the ultrafiltration sleeve assembly is a cellulose membrane, which is arranged in a cylindrical shape in the centrifuge tube body;

[0015] The lower part of the ultrafiltration sleeve assembly is a support member, through which the sample passes through the cellulose membrane and completes ultrafiltration.

[0016] Preferably, the support member has a conical pleated porous structure, the liquid outlet is located at the bottom of the support member, and the cellulose membrane is attached to the surface of the support member.

[0017] The protein ultrafiltration centrifuge tube for laboratory use provided by this utility model, as described above, has the following beneficial effects:

[0018] The present invention relates to a laboratory protein ultrafiltration centrifuge tube with a simple structure. The support is a conical pleated porous structure, and the cellulose membrane is attached to the surface of the support, which can increase the ultrafiltration membrane area. It can be used for laboratory protein sample ultrafiltration and concentration, with a small processing volume, high ultrafiltration efficiency, and easy sample collection with minimal loss. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0020] Figure 1 This is a schematic diagram of the structure of a laboratory protein ultrafiltration concentration centrifuge tube provided for an embodiment of the present invention.

[0021] Explanation of reference numerals in the attached figures:

[0022] 1. Cap; 2. Centrifuge tube body; 3. Ultrafiltration sleeve assembly; 4. Support component; 5. Threaded cap. Detailed Implementation

[0023] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0024] See Figure 1 As shown;

[0025] This invention relates to a laboratory protein ultrafiltration concentration centrifuge tube, which comprises:

[0026] Centrifuge tube body 2 has an open top structure; and

[0027] The cover 1 is connected to the upper part of the centrifuge tube body 2;

[0028] The centrifuge tube also includes:

[0029] The ultrafiltration sleeve assembly 3, which is located in the centrifuge tube body 2, has a liquid outlet at its bottom and a threaded cap 5 connected to the liquid outlet. The sample after ultrafiltration by the ultrafiltration sleeve assembly 3 flows out through the liquid outlet and is collected through the threaded cap 5.

[0030] As a preferred technical solution in this embodiment, the centrifuge tube body 2 is a 50 ml polypropylene pointed-bottom centrifuge tube, and the outside of the opening of the centrifuge tube body 2 is provided with external threads.

[0031] The cover 1 is made of polyethylene material and has internal threads, which are threaded to the centrifuge tube body 2.

[0032] As a preferred technical solution in this embodiment, the upper part of the ultrafiltration sleeve assembly 3 is a cellulose membrane, which is arranged in a cylindrical shape in the centrifuge tube body 2;

[0033] The lower part of the ultrafiltration sleeve assembly 3 is a support member 4, through which the sample passes through the cellulose membrane and completes ultrafiltration.

[0034] As a preferred technical solution in this embodiment, the support member 4 has a conical pleated porous structure, the liquid outlet is opened at the bottom of the support member 4, and the cellulose membrane is attached to the surface of the support member 4, which can increase the ultrafiltration membrane area.

[0035] This embodiment provides a laboratory protein ultrafiltration concentration centrifuge tube. In use, unscrew the cap 1, check if the threaded cap at the liquid outlet is tightened, add the sample to be ultrafiltered into the ultrafiltration sleeve assembly 3, tighten the cap 1, and place it in a centrifuge for centrifugation at a speed of 5000–10000 rpm. Determine the optimal centrifugation speed and time based on the sample type and concentration ratio. After centrifugation to the desired concentration volume, unscrew the cap 1, remove the ultrafiltration sleeve assembly 3, unscrew the threaded cap, and collect the target sample in the sample centrifuge tube.

[0036] The protein ultrafiltration centrifuge tube for laboratory use provided by this utility model, as described above, has the following beneficial effects:

[0037] The present invention relates to a laboratory protein ultrafiltration centrifuge tube with a simple structure. The support is a conical pleated porous structure, and the cellulose membrane is attached to the surface of the support, which can increase the ultrafiltration membrane area. It can be used for laboratory protein sample ultrafiltration and concentration, with a small processing volume, high ultrafiltration efficiency, and easy sample collection with minimal loss.

[0038] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A laboratory protein ultrafiltration centrifuge tube, characterized in that, The centrifuge tube includes: The centrifuge tube body (2) has an open top structure; and The cover (1) is connected to the upper part of the centrifuge tube body (2); The centrifuge tube also includes: The ultrafiltration sleeve assembly (3) located in the centrifuge tube body (2) has a liquid outlet at its bottom and a threaded cap (5) connected to the liquid outlet. The sample after ultrafiltration by the ultrafiltration sleeve assembly (3) flows out through the liquid outlet and is collected through the threaded cap (5).

2. The laboratory protein ultrafiltration concentration centrifuge tube according to claim 1, characterized in that, The centrifuge tube body (2) is made of 50 ml polypropylene pointed bottom centrifuge tube, and the opening of the centrifuge tube body (2) is provided with external threads. The cover (1) is made of polyethylene material and has internal threads that are threaded to the centrifuge tube body (2).

3. The laboratory protein ultrafiltration concentration centrifuge tube according to claim 1, characterized in that, The upper part of the ultrafiltration sleeve assembly (3) is a cellulose membrane, which is arranged in a cylindrical shape in the centrifuge tube body (2); The lower part of the ultrafiltration sleeve assembly (3) is a support member (4), and the sample passes through the cellulose membrane and is ultrafiltered by the support member.

4. A laboratory protein ultrafiltration centrifuge tube according to claim 3, characterized in that, The support member (4) has a conical pleated porous structure, the liquid outlet is opened at the bottom of the support member (4), and the cellulose membrane is attached to the surface of the support member (4).

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