Constant temperature device and gene detection equipment
By designing a temperature control device consisting of a constant temperature bath and a flow equalizer in the gene detection equipment, the problem of inaccurate detection results caused by uneven chip temperature was solved, achieving the effects of temperature uniformity and reduced energy consumption.
Patent Information
- Application Number
- CN202520332830.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2035-02-28
AI Technical Summary
In existing gene testing equipment, uneven chip temperature leads to inaccurate test results.
Design a temperature control device by opening a temperature control tank on the bottom wall of the medium tank and setting a flow equalization block on the cover plate to ensure that the flow area is consistent at all positions in the medium channel, thereby achieving temperature consistency for each chip.
It improves the uniformity of chip temperature, enhances the accuracy and reliability of detection results, and reduces energy consumption.
Smart Images
Figure CN223841581U_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the field of chip temperature control equipment technology, specifically relating to a temperature control device and a gene detection device. Background Technology
[0002] Staining and washing are crucial steps in gene detection processes. Staining, typically performed during sample preparation, involves transcribing extracted total RNA into fluorescently labeled cDNA using reverse transcriptase, facilitating subsequent detection of gene expression. Washing removes unbound or poorly hybridized cDNA to reduce interference and improve the accuracy of results.
[0003] The chemical reactions involved in washing and staining require suitable temperature conditions. If the chip temperature is too high, problems such as excessive antigen-antibody binding, increased nucleic acid hybridization mismatches, intensified fluorescence quenching, and denaturation of fluorescent genes may occur. If the chip temperature is too low, problems such as insufficient antigen-antibody binding, incomplete nucleic acid hybridization, increased liquid viscosity, and crystallization and precipitation may occur. These problems can affect the accuracy of the test results. Utility Model Content
[0004] Therefore, there is an urgent need for a constant temperature device and gene detection equipment that can ensure the chip temperature is appropriate during the washing and staining process.
[0005] The technical solution proposed in this application is as follows:
[0006] A constant temperature device, comprising:
[0007] The carrier plate has a first side and a second side. The first side is provided with a plurality of carrier positions for carrying chips. The second side is provided with a dielectric groove. The bottom wall of the dielectric groove is provided with a plurality of constant temperature grooves. Each constant temperature groove corresponds to one of the carrier positions.
[0008] The cover plate has multiple flow equalization blocks on one side. The cover plate is sealed to the second side to enclose the medium tank and form a medium flow channel. Each flow equalization block can extend into the medium tank and corresponds to a constant temperature tank.
[0009] Using the aforementioned temperature control device, the chip is placed on the support position. The temperature-controlled medium flows within the medium channel, exchanging heat with the chip on the support position during the flow. The uniform flow block ensures that the flow area at each position of the medium channel is basically the same, thereby ensuring that the heat exchange effect between each temperature control bath and the corresponding support position is basically consistent. This ensures the consistency of chip temperature at each support position, keeping all chips at a suitable temperature, and thus improving the accuracy of the detection results.
[0010] Furthermore, the medium tank includes multiple heat exchange tanks and multiple connecting tanks. Each heat exchange tank extends along a first direction, and the multiple heat exchange tanks are spaced apart along a second direction perpendicular to the first direction. The ends of any two adjacent heat exchange tanks are connected through the connecting tanks to form a tortuous medium tank. The constant temperature tank is formed on the bottom wall of the heat exchange tank.
[0011] Furthermore, the bottom wall of each heat exchange tank is provided with a plurality of constant temperature tanks spaced apart along the first direction.
[0012] Furthermore, the support plate or the cover plate has a medium inlet and a medium outlet, and the medium inlet and the medium outlet are respectively connected to both ends of the medium flow channel.
[0013] Furthermore, the horizontal height of the medium flow channel gradually increases from the end where the medium inlet is located to the end where the medium outlet is located.
[0014] Furthermore, the distance between the bottom wall of the constant temperature bath and the bearing position is greater than 0 mm and less than 1.5 mm.
[0015] Furthermore, the first side is provided with a plurality of bearing protrusions, and the bearing position is disposed on the bearing protrusions.
[0016] Furthermore, the plurality of bearing protrusions are spaced apart along the first direction, and each bearing protrusion is provided with a plurality of bearing positions spaced apart along a second direction perpendicular to the first direction.
[0017] A gene detection device, including the temperature control device described above.
[0018] Furthermore, it also includes a temperature detector, which is disposed at the bearing position.
[0019] The constant temperature device and gene detection equipment provided in this application have at least the following advantages:
[0020] 1. A constant temperature bath is opened on the bottom wall of the medium tank to reduce the wall thickness of the support plate at the constant temperature bath. The support position for placing the chip corresponds to the constant temperature bath, thereby improving the heat exchange effect and reducing energy consumption while ensuring the structural strength of the support plate.
[0021] 2. The cover plate is equipped with a flow equalization block, which can extend into the medium tank and correspond to the constant temperature tank, so that the flow area of each position of the medium channel is consistent. At the same time, the flow area of the medium inlet and medium outlet is also consistent with the flow area of the medium channel, thereby avoiding the situation where some liquids do not flow due to eddies, improving the uniformity of temperature distribution, and thus improving the accuracy and reliability of the test results.
[0022] 3. The horizontal height of the medium flow channel gradually increases from the end where the medium inlet is located to the end where the medium outlet is located, which facilitates exhaust, reduces workload, and improves operating efficiency. Attached Figure Description
[0023] The accompanying drawings are provided to further understand this application and form part of the specification. They are used together with the embodiments of this application to explain this application and do not constitute a limitation thereof.
[0024] Figure 1 This is a schematic diagram of the structure of a constant temperature device provided in an embodiment of this application;
[0025] Figure 2 for Figure 1 A schematic diagram of the structure of the second side of the support plate in the constant temperature device shown;
[0026] Figure 3 for Figure 1 The diagram shows the structure of the cover plate in the constant temperature device.
[0027] Figure 4 for Figure 1 A top view of the thermostat device shown.
[0028] Figure 5 for Figure 4 A schematic cross-sectional view of the thermostat at point AA;
[0029] Figure 6 for Figure 4 A schematic cross-sectional view of the temperature control device at point BB.
[0030] Label Explanation:
[0031] 100, Support plate; 110, Medium flow channel; 111, Medium tank; 112, Constant temperature tank; 113, Heat exchange tank; 114, Connecting groove; 120, Support protrusion; 130, Medium inlet; 140, Medium outlet; 200, Cover plate; 210, Flow equalization block. Detailed Implementation
[0032] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.
[0033] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the equipment or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.
[0034] On the one hand, this application provides a constant temperature device that can be used for placing chips in gene detection processes to ensure that the chip temperature is suitable during washing and staining steps, thereby ensuring the accuracy and reliability of the detection results.
[0035] like Figures 1 to 3 As shown, in one embodiment, the temperature control device includes a carrier plate 100 and a cover plate 200. The carrier plate 100 has a first side and a second side facing each other. The first side is provided with a plurality of carrier positions for carrying chips; the second side has a dielectric groove 111, the extension path of which can correspond to each carrier position. Please also refer to... Figure 4 and Figure 5 The cover plate 200 is sealed to the second side to enclose the medium tank 111, forming a medium flow channel 110. In this way, a constant-temperature medium can flow within the medium flow channel 110 to achieve heat exchange with the chip at the support position. Optionally, the constant-temperature medium is water, oil, or other heat exchange media.
[0036] Furthermore, the bottom wall of the medium tank 111 is provided with a plurality of constant temperature tanks 112, each constant temperature tank 112 corresponding to a bearing position, that is, the constant temperature medium flowing into the constant temperature tank 112 can exchange heat with the chip on the bearing position; at the same time, the opening of the constant temperature tank 112 reduces the wall thickness of the bearing plate 100 where the constant temperature tank 112 is located, and reduces the distance between the constant temperature medium and the chip, which can improve the heat exchange effect.
[0037] Furthermore, a plurality of flow equalization blocks 210 are provided on one side of the cover plate 200. When the cover plate 200 is connected to the second side, each flow equalization block 210 can extend into the medium tank 111 and correspond to a constant temperature tank 112. In this way, during the flow of the constant temperature medium along the medium flow channel 110, the flow equalization blocks 210 can make the medium flow area at the constant temperature tank 112 close to or even consistent with the flow area at other positions of the medium flow channel 110, thereby making the heat exchange effect consistent at all positions of the medium flow channel 110, that is, the heat exchange effect consistent at each constant temperature tank 112, and thus making the temperature of multiple chips on multiple bearing positions consistent, ensuring that the temperature of each chip is at a suitable temperature, and improving the accuracy of the detection results.
[0038] It should be explained that without the thermostatic bath 112, the depth of the medium tank 111 cannot be too large to ensure the structural strength of the thermostatic device. Therefore, the distance between the bottom wall of the medium tank 111 and the support position is relatively large, resulting in poor heat exchange and high energy consumption. However, with the thermostatic bath 112, the distance between the bottom wall of the thermostatic bath 112 and the support position is smaller, which can effectively improve the heat exchange effect and reduce energy consumption costs. Furthermore, with the thermostatic bath 112, if the flow equalization block 210 is not set, eddies may occur in the thermostatic bath 112, causing some liquids in the thermostatic bath 112 to not flow, affecting the thermostatic effect. If the flow equalization block 210 is set, the flow area at each position in the medium channel 110 is basically the same, which can effectively avoid the formation of eddies in the thermostatic bath 112 and ensure the consistency of chip temperature at each support position.
[0039] In summary, using the aforementioned temperature control device, the chip is placed on the support position, and the temperature-controlled medium flows within the medium flow channel 110, exchanging heat with the chip on the support position during the flow. The arrangement of the flow equalization block 210 ensures that the flow area at each position of the medium flow channel 110 is basically the same, thereby ensuring that the heat exchange effect between each temperature-controlled bath 112 and the corresponding support position is basically the same. This ensures the consistency of the chip temperature at each support position, keeping all chips at a suitable temperature, and thus improving the accuracy of the detection results.
[0040] Furthermore, when multiple chips are used for simultaneous detection, the consistent temperature of the multiple chips prevents temperature from affecting the detection results, thereby improving the accuracy of the detection results and verifying the generalizability of the detection results.
[0041] Furthermore, the distance between the bottom wall of the constant temperature bath 112 and the bearing position is greater than 0 mm and less than 1.5 mm, meaning the wall thickness of the bearing plate 100 at the constant temperature bath 112 is greater than 0 mm and less than 1.5 mm. The wall thickness at the constant temperature bath 112 is less than the wall thickness at other locations on the bearing plate 100. Therefore, while ensuring the overall structural strength of the bearing plate 100, the heat exchange effect between the constant temperature bath 112 and the bearing position can be improved, reducing energy consumption. For example, the distance between the bottom wall of the constant temperature bath 112 and the bearing position can be 0.5 mm, 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1 mm, etc.
[0042] In one embodiment, a plurality of bearing protrusions 120 are provided on the first side, and bearing positions are disposed on the bearing protrusions 120. Similarly, the constant temperature bath 112 is provided corresponding to the bearing protrusions 120, so as to reduce the wall thickness of the bearing plate 100 at the constant temperature bath 112 while ensuring structural strength, thereby improving the heat exchange effect.
[0043] Furthermore, multiple support protrusions 120 are spaced apart along a first direction, and each support protrusion 120 has multiple support positions spaced apart along a second direction perpendicular to the first direction. This improves work efficiency, and the array arrangement of multiple support positions along the first and second directions enhances the convenience of chip placement and removal. Specifically... Figure 1 In the embodiment shown, the first direction is the length direction of the support plate 100, and the second direction is the width direction of the support plate 100.
[0044] In one embodiment, the medium tank 111 includes multiple heat exchange tanks 113 and multiple connecting tanks 114. Each heat exchange tank 113 extends along a first direction, and the multiple heat exchange tanks 113 are spaced apart along a second direction. The ends of any two adjacent heat exchange tanks 113 are connected through the connecting tanks 114 to form a tortuous medium tank 111. In other words, for any three adjacent heat exchange tanks 113, the two ends of the middle heat exchange tank 113 are respectively connected to the ends of the other two heat exchange tanks 113 through the connecting tanks 114, and the connecting tanks 114 are connected to the same end of any two adjacent heat exchange tanks 113. A constant temperature tank 112 is formed on the bottom wall of the heat exchange tank 113.
[0045] Furthermore, each heat exchange tank 113 has multiple constant temperature tanks 112 spaced apart along the first direction on its bottom wall, and the constant temperature tanks 112 of the multiple heat exchange tanks 113 correspond one-to-one. In this way, the multiple constant temperature tanks 112 can correspond one-to-one with the multiple bearing positions arranged in an array along the first direction and the second direction.
[0046] In one embodiment, the support plate 100 or cover plate 200 has a medium inlet 130 and a medium outlet 140, which are respectively connected to the two ends of the medium flow channel 110 to allow the constant temperature medium to be input from the medium inlet 130 and output from the medium outlet 140. The constant temperature medium flows through the heating or cooling mechanism outside the medium flow channel 110 and then flows back into the medium flow channel 110 through the medium inlet 130, realizing the circulation of the constant temperature medium and ensuring the constant temperature effect. Specifically... Figure 1 In the embodiment shown, both the medium inlet 130 and the medium outlet 140 are located on the support plate 100 and on the side surface of the support plate 100 adjacent to the second side.
[0047] Please see Figure 5 and Figure 6In one embodiment, in the first direction, the length of the flow equalization block 210 is less than the length of the constant temperature bath 112, while in the second direction, the dimensions of the heat exchange bath 113, the constant temperature bath 112, and the flow equalization block 210 are all the same. Thus, the flow equalization block 210 extends into the heat exchange bath 113, effectively sealing it. Since the top of the flow equalization block 210 extends into the constant temperature bath 112 but does not seal it, the flow equalization block 210, together with the heat exchange bath 113 and the constant temperature bath 112, forms a flow channel that extends along the first direction and undulates vertically, ensuring the consistency of the flow area at each position of the flow channel.
[0048] In one embodiment, the horizontal height of the medium flow channel 110 gradually increases from the end where the medium inlet 130 is located to the end where the medium outlet 140 is located. In this way, during initial use, by introducing a constant temperature medium through the medium inlet 130, the gas in the medium flow channel 110 can be easily discharged, reducing the workload.
[0049] In one embodiment, the flow areas of the medium inlet 130 and the medium outlet 140 are the same as the flow area of the medium channel 110 (within the allowable error range), so that the constant temperature medium in the medium channel 110 can flow fully, avoid eddies affecting the uniformity of temperature distribution, and ensure the accuracy of the detection results.
[0050] In one embodiment, the cover plate 200 and the support plate 100 are connected by screws, and the two can be sealed with glue.
[0051] On the other hand, this application also provides a gene detection device for performing gene detection. This gene detection device includes the temperature control device described in the above embodiments.
[0052] Furthermore, the gene detection device also includes a temperature detector, which is located at the support site to detect the temperature of the support site during the reaction process, so as to adjust the temperature of the isothermal medium according to the detection results and realize feedback regulation of the temperature of the support site.
[0053] In summary, the constant temperature device and gene detection equipment provided in this application have at least the following advantages:
[0054] 1. A constant temperature bath 112 is opened on the bottom wall of the medium bath 111 to reduce the wall thickness of the support plate 100 at the constant temperature bath 112. The support position for placing the chip corresponds to the constant temperature bath 112, thereby improving the heat exchange effect and reducing energy consumption while ensuring the structural strength of the support plate 100.
[0055] 2. A flow equalization block 210 is provided on the cover plate 200. The flow equalization block 210 can extend into the medium tank 111 and correspond to the constant temperature tank 112, so that the flow area of each position of the medium flow channel 110 is consistent. At the same time, the flow area of the medium inlet 130 and the medium outlet 140 is also consistent with the flow area of the medium flow channel 110, thereby avoiding the situation where eddies cause some liquids to stop flowing, improving the uniformity of temperature distribution, and thus improving the accuracy and reliability of the detection results.
[0056] 3. The horizontal height of the medium flow channel 110 gradually increases from the end where the medium inlet 130 is located to the end where the medium outlet 140 is located, which facilitates exhaust, reduces workload, and improves operating efficiency.
[0057] Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A constant temperature device, characterized in that, include: The carrier plate has a first side and a second side. The first side is provided with a plurality of carrier positions for carrying chips. The second side is provided with a dielectric groove. The bottom wall of the dielectric groove is provided with a plurality of constant temperature grooves. Each constant temperature groove corresponds to one of the carrier positions. The cover plate has multiple flow equalization blocks on one side. The cover plate is sealed to the second side to enclose the medium tank and form a medium flow channel. Each flow equalization block can extend into the medium tank and corresponds to a constant temperature tank.
2. The constant temperature device according to claim 1, characterized in that, The medium tank includes multiple heat exchange tanks and multiple connecting tanks. Each heat exchange tank extends along a first direction, and the multiple heat exchange tanks are spaced apart along a second direction perpendicular to the first direction. The ends of any two adjacent heat exchange tanks are connected through the connecting tanks to form a tortuous medium tank. The constant temperature tank is formed on the bottom wall of the heat exchange tank.
3. The constant temperature device according to claim 2, characterized in that, Each heat exchange tank has multiple constant temperature tanks spaced apart along the first direction on its bottom wall.
4. The constant temperature device according to claim 1, characterized in that, The support plate or the cover plate has a medium inlet and a medium outlet, and the medium inlet and the medium outlet are respectively connected to the two ends of the medium flow channel.
5. The constant temperature device according to claim 4, characterized in that, The horizontal height of the medium flow channel gradually increases from the end where the medium inlet is located to the end where the medium outlet is located.
6. The constant temperature device according to claim 1, characterized in that, The distance between the bottom wall of the constant temperature bath and the bearing position is >0mm and ≤1.5mm.
7. The constant temperature device according to claim 1, characterized in that, The first side is provided with multiple bearing protrusions, and the bearing position is disposed on the bearing protrusions.
8. The constant temperature device according to claim 7, characterized in that, The plurality of bearing protrusions are arranged at intervals along a first direction, and each bearing protrusion is provided with a plurality of bearing positions at intervals along a second direction perpendicular to the first direction.
9. A gene detection device, characterized in that, Includes the thermostatic device as described in any one of claims 1-8.
10. The gene detection device according to claim 9, characterized in that, It also includes a temperature detector, which is disposed at the bearing position.