Homogeneous chemiluminescence detection reagent disc, analyzer and detection system

Abstract

The utility model relates to the field of biomedical detection, in particular to a homogeneous chemiluminescence detection reagent disc, an analyzer and a detection system. Comprising a disposable circular or semicircular reagent disc body, and the reagent disc body is provided with a solution containing area, a reaction area and a reagent containing area along the edge of the circular track of the reagent disc body; the solution containing area comprises a plurality of solution containing cavities, the reaction area comprises a plurality of reaction cavities with light-proof side faces and bottoms, the reagent containing area at least comprises a plurality of reagent containing cavities with light-proof side faces and bottoms, and the solution containing cavities and the reagent containing cavities are correspondingly provided with solution reagents. Sealing films are arranged on the surfaces of the solution containing cavity and the reagent containing cavity in a sealed mode, and a lightproof sealing film is correspondingly attached to the lightproof reagent containing cavity. According to the invention, efficient and accurate homogeneous chemiluminescence detection is realized, the detection efficiency and accuracy are remarkably improved, and the stability and reliability of the system are ensured.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical detection instruments, in particular to a homogeneous chemiluminescence detection reagent disc, an analyzer and a detection system. BACKGROUND

[0002] The homogeneous chemiluminescence detection technology realizes homogeneous cleaning-free, rapid, high-sensitivity and high-throughput detection with its unique energy transfer mechanism and chemiluminescence principle, and can be applied to basic medical research, DNA molecule detection, food safety and new drug development and other fields. The reagent used in the technology contains two synthetic bead reagents and one biotin chemical monoclonal antibody. The first bead reagent (sensitive bead) is coated with streptavidin and contains a photosensitive dye; the second bead reagent (chemical bead) is coated with another antibody and contains a chemiluminescent dye; after incubation of the sample, the chemical bead and the biotinylated antibody, a sandwich complex is formed; then the sensitive bead is added, and after binding with the biotin, an aggregated immune complex is formed; under 680nm light irradiation, the sensitive bead in the complex will produce singlet oxygen, and the singlet oxygen will diffuse to the chemical bead to trigger a chemiluminescence reaction, and the chemiluminescence signal generated by the reaction is measured at 612nm wavelength.

[0003] Compared with the immunochromatography quantitative method, the homogeneous chemiluminescence technology has the advantages of high sensitivity and good repeatability, and compared with the traditional non-homogeneous chemiluminescence technology, the operation steps are relatively simple, and the instrument structure is not high, but the complex sample adding and detection structure still needs to be completed.

[0004] How to fully exert the advantages of the homogeneous chemiluminescence technology, so that the technology can be applied to the scene of POCT at low cost, realize the characteristics of simple instrument structure, small size, low production cost, stable performance and simple operation, which is of great significance to improve the medical detection technology level of grassroots medical institutions and popularize the application of new technology. Content of the utility model

[0005] The purpose of the present application is to provide a homogeneous chemiluminescence detection reagent disc, an analyzer and a detection system, and to improve the detection efficiency and accuracy.

[0006] In the first aspect, the homogeneous chemiluminescence detection reagent disc provided by the present application adopts the following technical scheme:

[0007] A homogeneous chemiluminescence detection reagent disc, comprising a disposable circular or semicircular reagent disc body, a solution containing area, a reaction area and a reagent containing area are arranged along the edge of the circular track of the reagent disc body;

[0008] The solution containing area comprises a plurality of solution containing cavities, the reaction area comprises a plurality of reaction cavities with opaque side and bottom, the reagent containing area comprises at least a plurality of reagent containing cavities with opaque side and bottom, the solution containing cavities and the reagent containing cavities are correspondingly provided with solution reagents, the surface of the solution containing cavities and the reagent containing cavities is provided with a sealing film, and the reagent containing cavities with opaque side and bottom are correspondingly provided with opaque sealing films.

[0009] By adopting the technical scheme, the reagent disc body is designed to arrange different functional areas or liquid containing areas required for homogeneous chemiluminescence detection in the edge of the circular track of the reagent disc body, the reagents, diluents, cleaning liquids, reaction cavities and the like required for homogeneous chemiluminescence detection are arranged in the edge of the circular track of the reagent disc body, the cavities required for homogeneous chemiluminescence detection and required to avoid light are provided with opaque side and bottom, the design can effectively prevent external light interference, improve the accuracy and reliability of the detection result, avoid optical crosstalk between different functional modules, ensure that the chemical reaction process in each module is not affected by external light, and improve the stability, detection precision and accuracy of the whole system.

[0010] Preferably, the reagent disc body is further provided with a suction head placing cavity, a sample containing cavity and a dilution cavity in the edge of the circular track thereof.

[0011] By adopting the technical scheme, the reagent disc body is further provided with a suction head placing cavity, a sample containing cavity and a dilution cavity, the linkage between the functional areas is stronger, the sample, the suction head and the dilution cavity required for homogeneous chemiluminescence detection are arranged in the edge of the circular track of the reagent disc body, and the efficiency of automatic operation is improved.

[0012] In the second aspect, the homogeneous chemiluminescence analyzer provided by the application adopts the following technical scheme:

[0013] A homogeneous chemiluminescence analyzer, the chemiluminescence analyzer comprises a liquid suction mechanism, a luminescence detection mechanism and a reagent disc mechanism;

[0014] The reagent disc mechanism comprises a driving assembly and a support for placing the homogeneous chemiluminescence analyzer test reagent disc of the first aspect, and the driving assembly is used to drive the reagent disc body and the support to rotate synchronously;

[0015] The liquid suction mechanism moves along the direction close to or away from the reagent disc body, and is used to transfer and mix the liquid contained in the reagent disc body;

[0016] The luminescence detection mechanism moves along the direction close to or away from the reagent disc body, and is used to detect the chemiluminescence signal of the reaction mixture in the reaction cavity of the reagent disc body.

[0017] By adopting the above technical solutions, the liquid aspiration mechanism of this application ensures the consistency and reliability of sample processing during the experiment through precise liquid aspiration and transfer within the reagent tray mechanism. The luminescence detection mechanism performs homogeneous chemiluminescence detection on the liquid within the reagent tray mechanism, achieving highly sensitive and accurate detection results. This improves the overall detection efficiency and accuracy. The reagent tray mechanism enables precise positioning and efficient rotation of the reagent tray body and its functional modules during the detection process. The design of the support component ensures stable support of the reagent tray body, while the introduction of the drive component allows the reagent tray body to rotate synchronously with the support component, thereby ensuring that each functional module can accurately connect with the liquid aspiration mechanism and the luminescence detection mechanism at predetermined positions, improving the system's automation level and detection efficiency.

[0018] Preferably, the drive assembly includes a drive motor, a first synchronous pulley, a synchronous belt, and a second synchronous pulley. The first synchronous pulley is coaxially fixed with the output shaft of the drive motor, the second synchronous pulley is coaxially fixed with the support member, and the synchronous belt is sleeved on the first and second synchronous pulleys.

[0019] By adopting the above technical solution, the combination of the drive motor, the first synchronous pulley, the synchronous belt, and the second synchronous pulley in this application enables precise synchronous rotation of the reagent tray body and its support. This design not only improves the stability and reliability of the system but also ensures precise alignment between various functional modules, thereby enhancing the accuracy and efficiency of homogeneous chemiluminescence detection. Specifically: the drive motor provides a stable power source, ensuring the continuity and stability of the rotational motion. The first synchronous pulley is coaxially fixed to the output shaft of the drive motor, achieving effective power transmission. The synchronous belt connects the first and second synchronous pulleys, ensuring smoothness during transmission. The second synchronous pulley is coaxially fixed to the support, allowing the support and the reagent tray body to rotate synchronously, avoiding positional deviations caused by asynchrony.

[0020] Preferably, the liquid aspiration mechanism includes a first lifting component and a liquid aspiration head component. The liquid aspiration head component is aligned with the functional module of the reagent tray body. The first lifting component is used to drive the liquid aspiration head component to move in a direction closer to or away from the reagent tray body.

[0021] By adopting the technical scheme, the liquid suction head assembly can accurately adjust the height position, ensure flexible switching between different functional modules, and improve the operation flexibility and working efficiency of the system. The liquid suction head assembly is aligned with the functional module of the reagent disc body, ensures accurate arrival at the target position each time, reduces human error, and improves the accuracy of the detection result. The whole design optimizes the liquid suction process, makes it more efficient and stable, and thus improves the reliability and precision of the whole homogeneous chemiluminescence detection system. Meanwhile, the liquid reaction process can be accurately operated by rotating the reagent disc mechanism and lifting the liquid suction mechanism, which simplifies the structure of the system and facilitates integration and miniaturization.

[0022] Preferably, the liquid suction head assembly is provided with a suction head connecting part at one end close to the reagent disc body, and the suction head connecting part is used for connecting the suction head.

[0023] By adopting the technical scheme, the provision of the suction head connecting part of the present application enables the liquid suction head assembly to conveniently and quickly replace the suction head, thereby ensuring the cleanliness and accuracy of each liquid suction operation and reducing the risk of cross contamination.

[0024] Preferably, the light emission detection mechanism comprises a second lifting assembly and a detection head assembly, the detection head assembly is aligned with the functional module of the reagent disc body, and the second lifting assembly is used to drive the detection head assembly to move along the direction close to or away from the reagent disc body.

[0025] By adopting the technical scheme, the second lifting assembly of the present application enables the detection head assembly to move up and down along the vertical direction, ensures accurate alignment of the detection head assembly with the target position, and improves the detection precision. The accurate positioning ability of the detection head assembly improves the detection efficiency and reduces the false detection and missed detection caused by inaccurate positioning. The overall structure design is compact and easy to operate, and improves the automation degree of the system.

[0026] Preferably, the detection head assembly comprises a light source emitting part, a collimating lens, a dichroic mirror, a first flat lens, a filter, a second flat lens and a light signal receiving plate.

[0027] The light source is emitted from the light source emitting part, passes through the collimating lens, the dichroic mirror and the first flat lens in sequence into the reaction cavity, and the light signal emitted after passing through the reaction mixture in the reaction cavity is received by the light signal receiving plate after passing through the first flat lens, the filter and the second flat lens in sequence.

[0028] By adopting the technical scheme, when the solution needs to be detected by the homogeneous chemiluminescence, the light source emitting part emits light to the reaction mixture in the reaction cavity of the reagent disc body, and then the light signal receiving plate receives the reflected light signal, so that the content of the substance to be detected in the reaction mixture is detected, and the detection accuracy is improved.

[0029] Preferably, the detection head assembly is further provided with a light shielding part matched with the reaction cavity.

[0030] By adopting the technical scheme, the light shielding part is used to avoid the interference of external light during detection, so that the light source emitting and light signal receiving process will not be affected by external light, and the accuracy of homogeneous chemiluminescence detection is improved.

[0031] Preferably, the chemiluminescence analyzer further comprises a punching mechanism for puncturing the sealing film on the surface of the reagent disc body.

[0032] The punching mechanism comprises a third lifting assembly and a punching head assembly, the punching head assembly is aligned with the functional module of the reagent disc body, and the third lifting assembly is used to drive the punching head assembly to move along the direction close to or away from the reagent disc body.

[0033] By adopting the technical scheme, the punching mechanism can effectively penetrate the sealing film on the reagent disc body, the third lifting assembly drives the punching head assembly to move along the direction close to or away from the reagent disc body, automatic operation is realized, and the running efficiency and stability of the system are improved.

[0034] Preferably, the support part is further provided with a constant temperature device inside.

[0035] By adopting the technical scheme, the constant temperature device inside the support part can effectively maintain the temperature environment in the reagent disc body stable, so that the homogeneous luminescence reagent can be maintained in the specified temperature range during detection, and the accuracy and reliability of the detection result are ensured.

[0036] In the second aspect, the homogeneous chemiluminescence detection system provided by the application adopts the following technical scheme:

[0037] A homogeneous chemiluminescence detection system comprises a disposable circular or semi-circular reagent disc body and a chemiluminescence analyzer, the reagent disc body is provided with a solution containing area, a reaction area and a reagent containing area along the edge of its circular track, the solution containing area comprises a plurality of solution containing cavities, the reaction area comprises a plurality of reaction cavities with opaque side and bottom, and the reagent containing area comprises a plurality of reagent containing cavities with opaque side and bottom, the solution containing cavities and the reagent containing cavities are correspondingly provided with solution reagents, the solution containing cavities and the reagent containing cavities are provided with sealing membranes on the surface, and the reagent containing cavities are correspondingly provided with opaque sealing membranes;

[0038] The chemiluminescence analyzer comprises a liquid suction mechanism, a luminescence detection mechanism and a reagent disc mechanism;

[0039] The reagent disc mechanism comprises a driving assembly and a support for placing the reagent disc body, and the driving assembly is used to drive the reagent disc body and the support to rotate synchronously;

[0040] The liquid suction mechanism moves along the direction close to or away from the reagent disc body, and is used to transfer and mix the liquid contained in the reagent disc body;

[0041] The luminescence detection mechanism moves along the direction close to or away from the reagent disc body, and is used to detect the chemiluminescence signal of the reaction mixture in the reaction cavity of the reagent disc body.

[0042] By adopting the above technical scheme, the reagent disc body design of the present application arranges the various functional areas required for homogeneous luminescence detection in order on the edge of the circular track of the reagent disc body, which facilitates the operation of the liquid suction mechanism and the luminescence detection mechanism, and improves the operation efficiency and accuracy. The liquid suction mechanism accurately sucks the liquid in the reagent disc mechanism, ensuring the consistency and reliability of sample processing during the experiment. The luminescence detection mechanism performs homogeneous chemiluminescence detection on the liquid in the reagent disc mechanism, achieving high sensitivity and accuracy of the detection results. Thus, the overall detection efficiency and accuracy of the system are improved.

[0043] In summary, the present application has at least one of the following beneficial technical effects:

[0044] 1. The reagent disc body of the present application is designed so that various elements required for homogeneous chemiluminescence detection, such as reagents, samples, diluents, cleaning liquids, reaction cavities, and suction heads, are arranged in order along the edge of the circular track of the reagent disc body, and efficient and flexible calling and processing can be performed above the edge of the reagent disc body by only rotating the reagent disc body, so that the punching, liquid suction, and detection function modules of the matching luminescence analysis instrument only need to move up and down to realize sample addition, sample dilution, reagent addition, mixing, cleaning of the suction head, and reaction detection of the reagent disc body, thereby making the instrument simple in structure, low in cost, and stable and reliable in performance.

[0045] 2. The cavity required for homogeneous chemical detection and required to avoid light is designed to be opaque to light, which can effectively prevent external light interference, improve the accuracy and reliability of the detection results, avoid optical crosstalk between different function modules, ensure that the chemical reaction process in each module is not affected by external light, and thereby improve the stability and detection precision of the entire system.

[0046] 3. The present application realizes a highly automated homogeneous chemiluminescence detection process by organically combining the reagent disc body and the analysis instrument, effectively improves the work efficiency, and reduces human errors.

[0047] 4. The present application can realize precise operation of the liquid reaction process by rotating the reagent disc mechanism and lifting the liquid suction mechanism, simplifying the structure of the system and facilitating integration and miniaturization. BRIEF DESCRIPTION OF DRAWINGS

[0048] Figure 1 is a homogeneous chemiluminescence detection reagent disc structure schematic diagram of an embodiment of the present application;

[0049] Figure 2 is a homogeneous chemiluminescence detection system structure schematic diagram of an embodiment of the present application;

[0050] Figure 3 is a liquid suction mechanism and punching mechanism structure schematic diagram of an embodiment of the present application;

[0051] Figure 4 is a luminescence detection mechanism structure schematic diagram of an embodiment of the present application;

[0052] Figure 5 is a detection head assembly structure schematic diagram of an embodiment of the present application;

[0053] In the figure, 1, reagent disc body; 11, suction head placement cavity; 12, sample containing cavity; 13, dilution cavity; 14, solution containing cavity; 15, reaction cavity; 16, reagent containing cavity; 2, analyzer; 21, liquid suction mechanism; 211, first lifting assembly; 212, liquid suction head assembly; 213, suction head connecting part; 22, luminescence detection mechanism; 221, second lifting assembly; 222, detection head assembly; 2221, light source emitting part; 2222, collimating lens; 2223, dichroic mirror; 2224, first flat lens; 2225, filter; 2226, second flat lens; 2227, light signal receiving plate; 23, reagent disc mechanism; 231, driving assembly; 232, supporting part; 24, punching mechanism; 241, third lifting assembly; 242, punching head assembly. DETAILED DESCRIPTION

[0054] The following description will be made in conjunction with the accompanying drawings Figure 1 - the accompanying drawings Figure 5 In order to clearly and completely describe the technical solutions of the present application, the described embodiments are only possible technical implementations of the present application, and not all possible implementations. Those skilled in the art can easily obtain other embodiments by combining the embodiments of the present application without creative labor, and these embodiments are also within the protection scope of the present application.

[0055] Embodiment 1:

[0056] The existing optical detection equipment is difficult to ensure the coordination and continuity between each link, resulting in low work efficiency and easy to make mistakes, and due to the lack of effective linkage mechanism between different functional modules, the operation process is tedious and time-consuming, which affects the overall detection efficiency and accuracy. Therefore, the present application provides a homogeneous chemiluminescence detection reagent disc, referring to Figure 1 , including a disposable circular or semicircular reagent disc body 1, the reagent disc body 1 is provided with a solution containing area, a reaction area and a reagent containing area along the edge of its circular track. The solution containing area includes a plurality of solution containing cavities 14, the reaction area includes a plurality of reaction cavities 15 which are opaque on the side and bottom, and the reagent containing area includes at least a plurality of reagent containing cavities 16 which are opaque on the side and bottom. The reagent disc body 1 is also provided with a suction head placement cavity 11, a sample containing cavity 12 and a dilution cavity 13 along the edge of its circular track. The solution containing cavities 14 and the reagent containing cavities 16 are correspondingly provided with solution reagents, and the surfaces of the solution containing cavities 14 and the reagent containing cavities 16 are provided with sealing membranes, and the opaque reagent containing cavities 16 are correspondingly attached with opaque sealing membranes.

[0057] As Figure 1As shown, the tip placement cavity 11, the sample containing cavity 12, the dilution cavity 13, the solution containing cavity 14, the reaction cavity 15 and the reagent containing cavity 16 of the embodiment of the application are arranged in the reverse clockwise direction on the edge of the reagent disc body 1. The tip placement cavity 11 is used to place a tip. The sample containing cavity 12 is used to contain a liquid sample to be detected. The dilution cavity 13 is used to dilute the liquid sample to be detected. The solution containing cavity 14 is used to contain a solution. A plurality of solution containing cavities 14 are uniformly arranged at the same interval on the edge of the reagent disc body 1. The solution containing cavities 14 are used to hold dilution liquid, cleaning liquid and the like required for detection. The reaction cavity 15 is used to provide a container for sample and reagent reaction. The reagent containing cavity 16 is used to contain a reagent. A plurality of reagent containing cavities 16 are uniformly arranged at the same interval on the edge of the reagent disc body 1. In the specific implementation process, black plastic material or other opaque materials can be selected to reduce the interference of external light.

[0058] The implementation principle of the embodiment is that the design of the reagent disc body 1 is such that the different functional areas or liquid containing areas required for homogeneous luminescence detection are arranged in order on the edge of the circular track of the reagent disc body 1, so that the reagents and steps required for homogeneous chemiluminescence detection are realized through the corresponding areas on the reagent disc body 1. At the same time, the cavities that need to be subjected to homogeneous chemical detection and need to be protected from light are set to be opaque. This design can effectively prevent external light interference, improve the accuracy and reliability of the detection results, avoid optical crosstalk between different functional modules, ensure that the chemical reaction process in each module is not affected by external light, thereby improving the stability and detection precision of the entire system, and effectively improving the detection efficiency and accuracy.

[0059] Embodiment 2

[0060] The existing optical detection system generally has the problems of complex operation, high manual intervention and low detection efficiency. Therefore, the application mainly adopts the following homogeneous chemiluminescence detection system, which includes a reagent disc body 1 and an analyzer 2, and achieves the effects of high automation and high detection efficiency. The application is further described in detail as follows.

[0061] The embodiment of the application provides a homogeneous chemiluminescence detection system, which refers to Figure 1 and Figure 2, including reagent disc body 1 and analyzer 2. The reagent disc body 1 is provided with solution containing area, reaction area and reagent containing area along its circular track edge. The solution containing area includes several solution containing cavities 14, the reaction area includes several reaction cavities 15 with opaque side and bottom, and the reagent containing area includes at least several reagent containing cavities 16 with opaque side and bottom. The reagent disc body 1 is also provided with suction head placing cavity 11, sample containing cavity 12 and dilution cavity 13 along its circular track edge. The solution containing cavities 14 and reagent containing cavities 16 are correspondingly provided with solution reagents, and the surfaces of the solution containing cavities 14 and reagent containing cavities 16 are provided with sealing membranes, and the opaque reagent containing cavities 16 are correspondingly attached with opaque sealing membranes. The chemiluminescence analyzer 2 includes liquid suction mechanism 21, luminescence detection mechanism 22 and reagent disc mechanism 23. The reagent disc mechanism 23 includes driving assembly 231 and support 232 for placing reagent disc body 1, and the driving assembly 231 is used to drive the synchronous rotation of the reagent disc body 1 and the support 232. The liquid suction mechanism 21 moves along the direction close to or away from the reagent disc body 1, and is used to transfer and mix the liquid contained in the reagent disc body 1. The luminescence detection mechanism 22 moves along the direction close to or away from the reagent disc body 1, and is used to detect the chemiluminescence signal of the reaction mixture in the reaction cavity 15 of the reagent disc body 1.

[0062] As shown in Figure 2 , the reagent disc mechanism 23 of the embodiment of the application includes driving assembly 231 and support 232, the support 232 is used to support the reagent disc body 1, and the driving assembly 231 is used to drive the synchronous rotation of the reagent disc body 1 and the support 232. The driving assembly 231 includes driving motor, first synchronous wheel, synchronous belt and second synchronous wheel, the first synchronous wheel is coaxially fixed with the output shaft of the driving motor, the second synchronous wheel is coaxially fixed with the support 232, and the synchronous belt is sleeved on the first synchronous wheel and the second synchronous wheel. In the specific implementation process, in order to improve the stability of rotation, a stepping motor or a servo motor can be selected as the driving motor, and a tensioning device can be added on the synchronous belt to ensure that the belt is always in a proper tension state.

[0063] As shown in Figure 3As shown, the liquid suction mechanism 21 of the embodiment of the present application comprises a first lifting assembly 211 and a liquid suction head assembly 212, the liquid suction head assembly 212 is aligned with the functional modules of the reagent disc body 1, and the first lifting assembly 211 is used to drive the liquid suction head assembly 212 to move along the direction of approaching or moving away from the reagent disc body 1. The liquid suction head assembly 212 is provided with a suction head connecting portion 213 at one end close to the reagent disc body 1, and the suction head connecting portion 213 is used to connect the suction head. In the specific implementation process, the suction head connecting portion 213 can adopt a threaded connection or a buckle connection mode, so as to facilitate the quick replacement of suction heads of different specifications. When the reagent disc body 1 is driven to rotate by the driving assembly 231, the suction head placing cavity 11 is first rotated to below the liquid suction mechanism 21, so that the liquid suction head assembly 212 brings up the suction head placed in the suction head placing cavity 11, thereby being able to drive the suction head to suck the liquid contained in the reagent disc body 1.

[0064] As shown in Figure 1 and Figure 3 As shown, the analyzer 2 of the embodiment of the present application further comprises a punching mechanism 24 to process the reagent disc body 1 with a sealing film. The punching mechanism 24 comprises a third lifting assembly 241 and a punching head assembly 242, the punching head assembly 242 is aligned with the functional modules of the reagent disc body 1, and the third lifting assembly 241 is used to drive the punching head assembly 242 to move along the direction of approaching or moving away from the reagent disc body 1. In the specific implementation process, the working principle of the punching mechanism 24 is as follows: when it is necessary to sample the reagent with a sealing film, the punching head assembly 242 is first controlled by the third lifting assembly 241 to descend to a proper position, and then the sealing film is punctured by the punching head assembly 242, so as to open the reagent. Then, the liquid suction mechanism 21 can suck the required amount of reagent through the suction head, and then transfer it to other containers. This process not only avoids the pollution risk caused by manual operation, but also effectively improves the work efficiency.

[0065] As shown in Figure 4 As shown, the light-emitting detection mechanism 22 of the embodiment of the present application comprises a second lifting assembly 221 and a detection head assembly 222, the detection head assembly 222 is aligned with the functional modules of the reagent disc body 1, and the second lifting assembly 221 is used to drive the detection head assembly 222 to move along the direction of approaching or moving away from the reagent disc body 1. In the specific implementation process, the detection head assembly 222 can be a light source transceiver device.

[0066] As shown in Figure 5As shown, the detection head assembly 222 includes a light source emitting part 2221, a collimating lens 2222, a dichroic mirror 2223, a first flat lens 2224, a filter 2225, a second flat lens 2226 and a light signal receiving plate 2227. The light source emits from the light source emitting part 2221, passes through the collimating lens 2222, the dichroic mirror 2223 and the first flat lens 2224 in sequence into the reaction chamber 15, and the light signal emitted after passing through the reaction mixture in the reaction chamber 15 passes through the first flat lens 2224, the filter 2225 and the second flat lens 2226 in sequence, and is received by the light signal receiving plate 2227. The detection head assembly (222) is also provided with a light shielding part cooperating with the reaction chamber (15) to avoid light interference from external sources during detection. The light source emission and light signal receiving processes are ensured not to be affected by external light through the light shielding part.

[0067] In the specific implementation process, when homogeneous chemiluminescence detection of the sample to be measured is required, the reagent disc body 1 is first installed in the support 232 of the analyzer 2, the sample to be measured is added to the sample containing cavity 12, and the suction head is placed in the suction head placing cavity 11. The constant temperature device in the support 232 heats and maintains the constant temperature of the reagent disc body 1. The solution containing cavity 14 of the reagent disc body 1 is pre-filled with the diluent and cleaning liquid required for detection, and the reagent containing cavity 16 is pre-filled with the reagent required for detection. Before detection, the punching mechanism 24 punches the sealing film on the solution containing cavity 14 and the reagent containing cavity 16. Through the rotation of the reagent disc body 1 and the up-down movement of the liquid suction mechanism 21, the operations of diluting the sample to be measured, adding the sample to the reaction chamber 15, adding the reagent to the reaction chamber 15, mixing the reaction mixture in the reaction chamber 15, and cleaning the suction head in the solution containing cavity between two sample adding operations are realized. After the reaction time is up, through the rotation of the reagent disc body 1 and the up-down movement of the luminescence detection mechanism 22, the detection of the light signal of the reaction mixture in the reaction chamber 15 is realized. At this time, the light source emitting part 2221 emits light to the reaction chamber 15 of the reagent disc body 1, usually a 680 nanometer light source, and then the luminescence detection mechanism 22 receives the chemiluminescence signal reflected by the reaction mixture in the reaction chamber 15. The filter 2225 is set to allow 610 nanometer light to pass through, so that the light signal receiving plate 2227 receives the 610 nanometer chemiluminescence signal. The intensity of the received 610 nanometer chemiluminescence signal is related to the content of the measured substance in the solution, thereby realizing the homogeneous luminescence detection of the sample to be measured. By pre-filling various reagents required for detection and providing multiple reaction chambers 15 and corresponding cleaning liquids, multiple detection items can be simultaneously detected for one sample on one reagent disc body.

[0068] The implementation principle of the embodiment is that: by uniformly arranging the various functional areas required for homogeneous luminescence detection on the reagent disc body 1 at certain intervals, and by driving the reagent disc body 1 to rotate accurately through the driving assembly 231, the manual intervention can be effectively reduced, and the detection efficiency can be improved. The design of the liquid suction mechanism 21 and the luminescence detection mechanism 22 makes the liquid processing and detection process more automated, reduces human error, and improves the accuracy and reliability of the detection results.

[0069] In another preferred embodiment, the light-tight design of the reagent disc body 1 can also be further optimized to improve the detection accuracy. In the specific implementation process, the light-tight design is implemented as follows: a layer of black or dark opaque coating can be coated on the outer side wall and the bottom surface of each light-tight cavity, or these parts can be made of light-tight materials. The purpose of this is to prevent external light from interfering with the light source collection in the detection process, thereby improving the accuracy and stability of the detection results.

[0070] In another preferred embodiment, a constant temperature device can also be arranged inside the support 232 to keep the temperature in the reagent disc body 1 stable.

[0071] The embodiments of the specific implementation are preferred embodiments of the present application, but do not limit the protection scope of the present application, wherein the same parts are denoted by the same reference numerals. Therefore: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A homogeneous chemiluminescent assay disc, characterized by, The application relates to a homogeneous chemiluminescence analysis test reagent disc for a homogeneous chemiluminescence analysis instrument, which comprises a disposable circular or semicircular reagent disc body (1) provided with a solution containing area, a reaction area and a reagent containing area along the circular track edge thereof. The solution containing area comprises a plurality of solution containing cavities (14), the reaction area comprises a plurality of reaction cavities (15) with opaque side surfaces and bottoms, and the reagent containing area comprises a plurality of reagent containing cavities (16) with opaque side surfaces and bottoms; the solution containing cavities (14) and the reagent containing cavities (16) are correspondingly provided with solution reagents; the surfaces of the solution containing cavities (14) and the reagent containing cavities (16) are provided with sealing membranes; and the reagent containing cavities (16) are correspondingly provided with opaque sealing membranes.

2. The homogeneous chemiluminescent test reagent disc according to claim 1, wherein, The reagent disc body (1) is further provided with a suction head placing cavity (11), a sample containing cavity (12) and a dilution cavity (13) along the circular track edge thereof.

3. A homogeneous chemiluminescent analyzer characterized by, The chemiluminescence analysis instrument (2) comprises a liquid suction mechanism (21), a luminescence detection mechanism (22) and a reagent disc mechanism (23). The reagent disc mechanism comprises a driving assembly (231) and a support (232) for placing the homogeneous chemiluminescence analysis instrument test reagent disc according to any one of claims 1-2; the driving assembly (231) is used for driving the reagent disc body (1) and the support (232) to rotate synchronously. The liquid suction mechanism (21) moves along the direction close to or away from the reagent disc body (1) to transfer and mix the liquid contained by the reagent disc body (1). The luminescence detection mechanism (22) moves along the direction close to or away from the reagent disc body (1) to detect the chemiluminescence signal of the reaction mixture in the reaction cavities (15) of the reagent disc body (1).

4. The homogeneous chemiluminescent analyzer according to claim 3, wherein The liquid suction mechanism (21) comprises a first lifting assembly (211) and a liquid suction head assembly (212); the liquid suction head assembly (212) is aligned with the circular track edge of the reagent disc body (1); and the first lifting assembly (211) is used for driving the liquid suction head assembly (212) to move along the direction close to or away from the reagent disc body (1). The liquid suction head assembly (212) is provided with a suction head connecting part (213) at one end close to the reagent disc body (1); and the suction head connecting part (213) is used for connecting a suction head.

5. The homogeneous chemiluminescent analyzer according to claim 4, wherein The luminescence detection mechanism (22) comprises a second lifting assembly (221) and a detection head assembly (222); the detection head assembly (222) is aligned with the circular track edge of the reagent disc body (1); and the second lifting assembly (221) is used for driving the detection head assembly (222) to move along the direction close to or away from the reagent disc body (1).

6. The homogeneous chemiluminescent analyzer according to claim 5, wherein The detection head assembly (222) comprises a light source emitting part (2221), a collimating lens (2222), a dichroic mirror (2223), a first flat lens (2224), a filter (2225), a second flat lens (2226) and a light signal receiving plate (2227). The light source emits from the light source emitting part (2221), sequentially passes through the collimating lens (2222), the dichroic mirror (2223) and the first flat lens (2224) into the reaction cavity (15), and the light signal emitted after passing through the reaction mixture in the reaction cavity (15) sequentially passes through the first flat lens (2224), the optical filter (2225) and the second flat lens (2226), and is received by the light signal receiving plate.

7. A homogeneous chemiluminescent assay according to claim 6, wherein, The detection head assembly (222) is further provided with a light shielding part matched with the reaction cavity (15).

8. A homogeneous chemiluminescence analyzer according to any one of claims 3-7, characterized in that, The chemical luminescence analyzer (2) further comprises a punching mechanism (24) for puncturing the sealing film on the surface of the reagent disc body (1). The punching mechanism (24) comprises a third lifting assembly (241) and a punching head assembly (242), the punching head assembly (242) is aligned with the circular track edge of the reagent disc body (1), and the third lifting assembly (241) is used to drive the punching head assembly (242) to move along the direction close to or away from the reagent disc body (1).

9. A homogeneous chemiluminescent assay according to any one of claims 3 to 7, wherein the chemiluminescent compound is a compound of formula (I) ###00003### (I) or a salt thereof. The inner part of the supporting part (232) is further provided with a constant temperature device.

10. A homogeneous chemiluminescent assay detection system characterized by, The chemical luminescence analyzer (2) comprises a disposable circular or semicircular reagent disc body (1) and a chemical luminescence analyzer (2), the reagent disc body (1) is provided with a solution containing area, a reaction area and a reagent containing area along the circular track edge thereof, the solution containing area comprises a plurality of solution containing cavities (14), the reaction area comprises a plurality of reaction cavities (15) which are opaque on the side and bottom, and the reagent containing area comprises a plurality of reagent containing cavities (16) which are opaque on the side and bottom, the solution containing cavities (14) and the reagent containing cavities (16) are correspondingly provided with solution reagents, the surfaces of the solution containing cavities (14) and the reagent containing cavities (16) are sealed with sealing films, and the reagent containing cavities (16) are correspondingly attached with opaque sealing films; The chemical luminescence analyzer (2) comprises a liquid sucking mechanism (21), a luminescence detection mechanism (22) and a reagent disc mechanism (23). The reagent disc mechanism comprises a driving assembly (231) and a supporting part (232) for placing the reagent disc body (1), and the driving assembly (231) is used to drive the reagent disc body (1) and the supporting part (232) to rotate synchronously. The liquid sucking mechanism (21) moves along the direction close to or away from the reagent disc body (1), and is used to transfer and mix the liquid contained in the reagent disc body (1). The luminescence detection mechanism (22) moves along the direction close to or away from the reagent disc body (1), and is used to detect the chemical luminescence signal of the reaction mixture in the reaction cavity (15) of the reagent disc body (1).

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