Optical microscope imaging module

Abstract

The utility model discloses an optical microscope imaging module, and belongs to the technical field of bio-photonics. The module comprises a bright field imaging module and a bright field module shell or a fluorescence imaging module and a fluorescence imaging module shell. The problem that an existing traditional optical microscope is poor in adaptability and fixed in structure in diversified experiment requirements is solved. According to the optical microscope imaging module, the flexibility and adaptability of a microscope system are greatly improved. Through modular arrangement, scientific researchers can quickly replace or carry various functional modules according to different experiment requirements, so that the microscope can more accurately meet the requirements of specific experiments.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the biophotonic technology field, more particularly to an optical microscope imaging module. BACKGROUND

[0002] As a core research tool in the fields of modern biology, medicine and material science, optical microscopes greatly help researchers to understand the microscopic world by visualizing subtle structures and dynamic processes.

[0003] However, traditional optical microscopes usually adopt fixed structures. Although these traditional devices have certain advantages in basic applications, they also face problems such as large size, single function, limited use scenarios, etc. Especially in the context of diversified experimental needs, the adaptability of microscopes is poor, and it is difficult to meet the matching between different experimental environments and needs. SUMMARY

[0004] The utility model aims at providing an optical microscope imaging module, which solves the limitations of poor adaptability and fixed structure of existing traditional optical microscopes in diversified experimental needs.

[0005] The utility model adopts the following technical scheme: an optical microscope imaging module, which comprises: a bright field imaging module and a bright field imaging module shell or a fluorescence imaging module and a fluorescence imaging module shell, the bright field imaging module is placed in the bright field imaging module shell, and the fluorescence imaging module is placed in the fluorescence imaging module shell.

[0006] Further, the bright field imaging module comprises: a bright field upper adapter, a bright field lower adapter, a filter drawer, an objective lens, a tube lens and a CCD camera.

[0007] The upper end of the bright field upper adapter is screwed with the objective lens through a threaded hole, and the lower end of the bright field upper adapter is coaxially provided with an inner threaded hole and an outer threaded hole; the lower end of the bright field upper adapter is connected with the tube lens through the inner threaded hole; the lower end of the tube lens is connected with the bright field lower adapter through the outer threaded hole, and the lower end of the bright field lower adapter is connected with the CCD camera; a groove is arranged on the side of the bright field upper adapter, which is used for placing the filter drawer.

[0008] Further, the bright field imaging module shell is composed of a bright field main body frame and a bright field door; a round port is arranged on the top of the bright field main body frame for the objective lens to protrude; a square port is arranged on the side of the bright field main body frame close to the bottom for connecting a data interface; a square support is arranged on the front end face of the bright field main body frame for fixing the bright field door.

[0009] Further, the fluorescence imaging module comprises: a fluorescence up-conversion interface, a fluorescence down-conversion interface, a light source shell, an objective lens, a tube lens, a dichroic mirror module, a dichroic mirror shell and a CCD camera; the light source shell is provided with a groove for placing a heat dissipation module and an LED light source; the outer side surface of the light source shell is provided with a threaded hole for fixing the fluorescence up-conversion interface; the upper end surface of the fluorescence up-conversion interface is connected with the objective lens through the threaded hole, and the tube lens is connected below the fluorescence up-conversion interface; the fluorescence down-conversion interface is connected below the tube lens, and the CCD camera is connected below the fluorescence down-conversion interface; the fluorescence up-conversion interface is provided with a groove for fixing the light source shell, and the side surface of the fluorescence up-conversion interface is provided with a round hole for input of the LED light source.

[0010] Further, the fluorescence imaging module shell is composed of a fluorescence main body frame and a fluorescence door; the top of the fluorescence main body frame is provided with a round port for the objective lens to extend out; and the side surface of the fluorescence main body frame is provided with a square port and a round hole near the bottom.

[0011] Further, the dichroic mirror shell is fixed to one side surface of the fluorescence up-conversion interface, and the dichroic mirror shell is composed of a bayonet, a dichroic mirror module and a latch; the dichroic mirror module is fixed in the dichroic mirror shell, and the outer side surface of the dichroic mirror shell is provided with a groove and a square port; the bayonet is provided with a square protrusion for fixing the latch; and the latch is provided with a square groove for fixing the bayonet.

[0012] Further, the tube lens is replaced by a 1X normal lens, and NA=0.025.

[0013] Further, the fluorescence up-conversion interface, the fluorescence down-conversion interface, the light source shell, the dichroic mirror shell, the bright field imaging module shell and the fluorescence imaging module shell are all made of aluminum alloy.

[0014] Further, the dichroic mirror module is divided into: a DAPI module, excitation wavelength: 350-365 nanometers, emission wavelength: 460-500 nanometers; an FITC module, excitation wavelength: 475-490 nanometers, emission wavelength: 510-560 nanometers; and a TRITC module, excitation wavelength: 525-545 nanometers, emission wavelength: 580-620 nanometers.

[0015] The optical microscope imaging module greatly improves the flexibility and adaptability of the microscope system. BRIEF DESCRIPTION OF DRAWINGS

[0016] Fig. 1 It is an overall structural schematic view of the bright field imaging module of the utility model;

[0017] Fig. 2It is the bright field imaging module structure schematic diagram of the utility model;

[0018] Fig. 3 It is the whole structure schematic diagram of the utility model fluorescent imaging module;

[0019] Fig. 4 It is the fluorescent imaging module structure schematic diagram of the utility model;

[0020] Fig. 5 It is the fluorescent imaging module disassembled structure schematic diagram of the utility model;

[0021] Fig. 6 It is the human red blood cell sample under 10 times, 20 times, 40 times of the bright field imaging module capture of the utility model;

[0022] Fig. 7 It is the human liver cancer cell sample under 10 times, 20 times, 40 times of the fluorescent imaging module capture of the utility model.

[0023] Wherein: 1, bright field main frame; 2, bright field door; 3, bright field L type adapter; 4, objective lens; 5, filter drawer; 6, tube lens; 7, bright field upper adapter; 8, bright field lower adapter; 9, CCD camera; 10, fluorescent main frame; 11, fluorescent door; 12, fluorescent L type adapter; 13, light source shell; 14, fluorescent upper adapter; 15, dichroic mirror shell; 16, buckle; 17, fluorescent lower adapter; 18, heat dissipation module; 19, dichroic mirror module; 20, bolt. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be clearly and completely described below, obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0025] The present application provides an optical microscope imaging module, which comprises: a bright field imaging module and a bright field imaging module shell or a fluorescent imaging module and a fluorescent imaging module shell, the bright field imaging module is arranged in the bright field imaging module shell, and the fluorescent imaging module is arranged in the fluorescent imaging module shell.

[0026] As shown in Figs. 1-2 , the bright field imaging module comprises: a bright field imaging module and a bright field imaging module shell.

[0027] In some embodiments, the bright field imaging module comprises: a bright field upper adapter 7, a bright field lower adapter 8, a filter drawer 5, an objective lens 4, a tube lens 6 and a CCD camera 9.

[0028] The upper adapter 7 of the bright field has three threaded holes, one on the top, two coaxially arranged on the bottom, inner and outer threaded holes, the objective lens 4 is screwed on the threaded hole on the top of the upper adapter 7 of the bright field, and the tube lens 6 is screwed on the inner threaded hole on the bottom of the upper adapter 7 of the bright field; the upper thread of the lower adapter 8 of the bright field is screwed on the outer threaded hole on the bottom of the upper adapter 7 of the bright field, and the CCD camera 9 is screwed on the lower threaded hole of the lower adapter 8 of the bright field; a groove is arranged on the upper adapter 7 of the bright field for placing the filter drawer 5, and the filter drawer 5 can place the required filter.

[0029] In some embodiments, the bright field module housing comprises a bright field main body frame 1 and a bright field door 2. The bright field main body frame 1 has a round opening on the top for the objective lens 4 to extend out, and two protruding square columns inside for fixing the bright field imaging module. A square opening is arranged on the lower left side of the bright field main body frame 1 for connecting the data interface. Four square supports are arranged on the four sides of the bright field main body frame 1, and threaded holes are arranged on the square supports for fixing the bright field door 2. Four threaded holes are arranged on the bright field door 2 for cooperation with the bright field main body frame 1.

[0030] The fluorescence imaging module comprises a fluorescence imaging module and a fluorescence imaging module housing.

[0031] As shown in Figs. 3-5 The fluorescence imaging module comprises a fluorescence upper adapter 14, a fluorescence lower adapter 17, a light source housing 13, an objective lens 4, a tube lens 6, a dichroic mirror module 19, a dichroic mirror housing 15, and a CCD camera 9.

[0032] The light source housing 13 has a recess inside and threaded holes outside, the recess inside is used for placing the heat dissipation module 18 and the LED light source, and the threaded holes outside are used for fixing the fluorescence upper adapter 14. The fluorescence upper adapter 14 has three threaded holes, one on the top and two coaxially arranged on the bottom, inner and outer threaded holes, the objective lens 4 is screwed on the threaded hole on the top of the fluorescence upper adapter 14, and the tube lens 6 is screwed on the inner threaded hole on the bottom of the fluorescence upper adapter 14; the fluorescence lower adapter 17 is screwed on the outer threaded hole on the bottom of the fluorescence upper adapter 14, and the CCD camera 9 is screwed on the lower threaded hole of the fluorescence lower adapter 9. The fluorescence upper adapter 14 has a recess inside for cooperation with the light source housing 13, and a round hole is arranged on the left side for input of the LED light source.

[0033] The dichroic mirror shell 15 is composed of a dichroic mirror module 19, a plug 20 and a bayonet 16. A groove is arranged in the dichroic mirror shell 15 for fixing the dichroic mirror module 19, and the dichroic mirror module 19 is externally provided with two grooves and two square openings, and the external grooves are internally provided with a cylinder for placing a spring; the front of the bayonet 16 is provided with a square protrusion for matching the groove in the plug 20; the front of the plug 20 is left with a square groove for matching the bayonet 16 for fixation, and the lower part is provided with a cylinder for matching the spring.

[0034] The fluorescence imaging module shell is composed of a fluorescence main body frame 10 and a fluorescence door 11. The top of the fluorescence main body frame 10 is provided with a round opening for the extension of the objective lens 4; the inside of the fluorescence main body frame 10 is provided with two protruding square columns and a pressure plate interface, and the square columns are provided with threaded holes for the fixation of the fluorescence imaging module. The left lower part of the fluorescence main body frame 10 is provided with a square opening and a circular hole, the square opening is used for the connection of a data interface, and the circular hole is used for the connection of a power supply port. The side of the fluorescence main body frame 10 is provided with four square support openings with threaded holes for fixing the fluorescence door 11. The fluorescence door 11 is provided with four threaded holes for matching the fluorescence main body frame 10.

[0035] The objective lens 4 is a semi-composite achromatic objective lens, which can be selected from 5X, 10X, 20X and 40X. The 5X semi-composite achromatic objective lens has an NA of 0.15 and a WD of 20; the 10X semi-composite achromatic objective lens has an NA of 0.3 and a WD of 11; the 20X semi-composite achromatic objective lens has an NA of 0.45 and a WD of 3; and the 40X semi-composite achromatic objective lens has an NA of 0.8 and a WD of 1.

[0036] The tube lens 6 is replaced by a 1X normal lens, and has an NA of 0.025.

[0037] The CCD camera 9 has a spectral response range of 380-650nm, adopts a USB3.0 interface, has a working temperature of-10-50℃, a working humidity of 30-80%RH, a sensor model of 5.1M / MT9P006(C), a pixel of 2.2*2.2um, a light sensitivity dynamic range signal-to-noise ratio of 1.76v / lux-sec 67.74dB, and an exposure time of 0.1ms-2000ms.

[0038] The bright field upper adapter 7, the bright field lower adapter 8, the fluorescence upper adapter 14, the fluorescence lower adapter 17, the light source shell 13, the dichroic mirror shell 15, the bright field imaging module shell and the fluorescence imaging module shell are all made of aluminum alloy materials.

[0039] The heat dissipation module 18 includes a heat dissipation fan and an aluminum alloy heat dissipation block. The heat dissipation fan has a size of 25*25*10mm, is made of aluminum, has good heat conduction performance, is light in quality and small in density, and has a working voltage of 12V.

[0040] LED light source is divided into purple light, blue light and green light. The working voltage is 3-3.7V. Purple light 3W, wavelength 365-370nm; blue light 3W, wavelength 485-490nm; green light 3W, 520-525nm.

[0041] Dichroic mirror module, divided into three. Respectively:

[0042] DAPI module: excitation wavelength: 350-365nm, emission wavelength: 460-500nm.

[0043] FITC module: excitation wavelength: 475-490nm, emission wavelength: 510-560nm.

[0044] TRITC module: excitation wavelength: 525-545nm, emission wavelength: 580-620nm.

[0045] Bright field imaging module and fluorescence imaging module two types of main difference lies in the use of different scenarios and whether need dichroic mirror module.

[0046] A method for working an optical microscope imaging module, comprising the following steps:

[0047] Step 1, bright field imaging module: first, the tube lens 6 is screwed into the inner thread under the bright field imaging module bright field on adapter 7. Then, the bright field on adapter 7 and the bright field under adapter 8 are screwed together. Then, the CCD camera 9 is screwed under the bright field under adapter 8. Finally, the bright field imaging module is fixed on the bright field imaging module shell and fixed with the bright field door 2. After the above steps, the objective lens 4 is screwed onto the bright field imaging module, and the installation is completed.

[0048] Or fluorescence imaging module: the tube lens 6 is screwed into the inner thread under the fluorescence on adapter 14. Then, the fluorescence on adapter 14 and the fluorescence under adapter 17 are screwed together. Then, the CCD camera 9 is screwed under the fluorescence under adapter 17. Then the light source shell is matched on the left side of the fluorescence on adapter 14, and the dichroic mirror module 19 is inserted into the dichroic mirror shell 15 and inserted into the fluorescence on adapter 14. Finally, the fluorescence imaging module is fixed on the fluorescence imaging module shell and fixed with the fluorescence door 11. After the above steps, the objective lens 4 is screwed onto the fluorescence imaging module, and the installation is completed.

[0049] Further, the bright field L-shaped adapter 3 is fixed in place or on the gantry, and the bright field imaging module shell or the fluorescence imaging module shell is fixed on the L-shaped adapter.

[0050] Step 2, open the visualization data acquisition software, connect the CCD camera 9 interface, select the required resolution and white balance state, and use the image enhancement algorithm to process the real-time picture in the subsequent imaging stage.

[0051] Step 2 specific steps as follows:

[0052] Step a, remove the noise in the image by Gaussian filtering, especially high frequency noise. By weighted average of image, the pixel value of image is smoothed according to the pixel value of its neighborhood. The formula of Gaussian function is as follows:

[0053] (1)

[0054] Where, σ is the standard deviation of Gaussian function, x represents the horizontal coordinate of pixel, and y represents the vertical coordinate of pixel.

[0055] The normalized coefficient represents the smoothing degree of the filter. By using multiple Gaussian filters with different standard deviations to filter the image, background information of different scales is obtained.

[0056] Specific steps:

[0057] First scale filtering: using Gaussian filter with standard deviation of 50 to smooth the gray image, and get the first scale background image.

[0058] Second scale filtering: using Gaussian filter with standard deviation of 100 to smooth the gray image, and get the second scale background image.

[0059] Multi-scale background fusion: average fusion of background images of two scales, and get the comprehensive background image.

[0060] Through the above multi-scale Gaussian filtering, the large scale background structure in the image is captured, which provides accurate reference for subsequent local light intensity adjustment.

[0061] Step b, histogram equalization, by adjusting the gray level distribution of image, the contrast of image is more balanced, so as to enhance the details. However, the traditional histogram equalization will remap the gray level of the whole image, which may lead to excessive enhancement of the contrast of some local area and produce noise. In order to solve this problem, CLAHE is proposed. The formula is as follows:

[0062] (2)

[0063] In formula 2,

[0064] r k is the gray level of input image;

[0065] n i is the number of pixels in the image with gray level i;

[0066] n is the total number of pixels in the image.

[0067] represents the cumulative distribution function (CDF) of gray level rk, which is used to map the original gray values to new gray values.

[0068] K: represents the index of the current gray level, which is used to calculate the cumulative distribution function.

[0069] By dividing the image into multiple small regions (tiles) and performing histogram equalization on each region separately, the local contrast of the image can be significantly improved, especially in low-contrast or high-noise images, making the details more clear.

[0070] Step c, enhance the high-frequency components of the image, especially the edge information in the image, through the de-sharpening mask, so that the edges are more prominent and the image becomes more sharp.

[0071] The formula is as follows:

[0072] (3)

[0073] In formula 3,

[0074] I sharpened : is the image after sharpening.

[0075] I is the original image (unprocessed image).

[0076] I blur is the image after blurring, i.e. the result of low-pass filtering.

[0077] K is the sharpening coefficient, which controls the intensity of enhancement, usually a positive value. If k is larger, the sharpening effect is stronger and the edges are more obvious; if k is smaller, the effect is weaker.

[0078] The process of the de-sharpening mask can be divided into three steps. First, by blurring the original image (such as Gaussian filtering or mean filtering), the low-frequency components of the image are obtained, and the blurred image presents a smooth background, lacking in detail information. Then, subtract the blurred image from the original image to obtain a difference image, which represents the high-frequency components in the original image, i.e. the edges and details, extracting the edge information of the image. Finally, multiply this high-frequency difference image by an enhancement coefficient k and add it back to the original image, thereby enhancing the edges and details of the image and making the image more sharp.

[0079] In summary, the above image processing steps are completed, the noise is removed, the contrast is improved and the edge details are enhanced, thereby effectively improving the clarity and visual effect of the image, and providing a reliable basis for subsequent image analysis tasks.

[0080] Step 3, after connecting all the ports, according to the actual shooting situation, the selection of the number of cycles and the interval time, finally the collected data is saved to the designated folder.

[0081] Example 1

[0082] Working process of bright field imaging module

[0083] First, screw the tube lens 6 into the inner thread under the bright field upper adapter 7. Then, screw the bright field upper adapter 7 with the bright field lower adapter 8. Then, screw the CCD camera 9 under the bright field lower adapter 8. Finally, fix the bright field imaging module on the bright field imaging module shell, and fix it with the bright field door 2. After the above steps, screw the 10x objective lens onto the bright field imaging module, and complete the installation.

[0084] Further, fix the bright field L-shaped adapter 3 on the moving platform, and then fix the bright field imaging module shell and the bright field imaging module on the bright field L-shaped adapter 3.

[0085] Place the collected red blood cell sample on the sample holder above the moving platform, open the visual data acquisition software, connect the CCD camera 9 interface, select the required resolution and white balance state, and use the image enhancement algorithm to process the real-time picture in the subsequent imaging stage; move the moving platform, select the number of cycles and the interval time according to the actual shooting situation, and finally save the collected data to the designated folder; replace the objective lens 4 with 20x and 40x respectively, and collect data respectively, and save the collected data to the designated folder. Fig. 6 Human red blood cell samples under 10x, 20x and 40x respectively.

[0086] Example 2

[0087] Working process of fluorescence imaging module: first, screw the tube lens 6 into the inner thread of the fluorescence upper adapter 14. Then, screw the fluorescence upper adapter 14 with the fluorescence lower adapter 17. Then, screw the CCD camera 9 under the fluorescence lower adapter 17, fix the light source shell on the left side of the fluorescence upper adapter 14, and insert the DIPA dichroic mirror module 19 into the dichroic mirror shell 15 and then into the fluorescence upper adapter 14. Finally, fix the fluorescence imaging module in the fluorescence imaging module shell, and complete the fixation with the fluorescence door 11. After the above steps, screw the 10x objective lens onto the fluorescence imaging module, and complete the installation.

[0088] Further operation, the fluorescent L-shaped adapter 12 is fixed on the mobile platform, and then the fluorescent imaging shell and the fluorescent imaging module are installed on the fluorescent L-shaped adapter 12.

[0089] The DIPA dyed human liver cancer cell sample is placed in the sample holder above the mobile platform, the visualization data acquisition software is started, and the CCD camera 9 interface is connected. Select the required resolution and white balance state, and the subsequent imaging stage will automatically use the image enhancement algorithm to process the real-time picture. Adjust the sample position through the mobile platform, set the cycle number and shooting interval time, and save the collected data to the specified folder.

[0090] After completing the collection under the 10x objective lens, the objective lens 4 is replaced with 20x and 40x respectively, and the data collection process is repeated, and the data is saved to the specified folder. Fig. 7 The imaging effect of the human liver cancer cell sample under 10x, 20x and 40x magnification is shown.

[0091] In addition, the modularity can also be deeply integrated with automation technology, further improving the operation convenience and experimental efficiency of the microscope. By integrating image enhancement algorithms and data acquisition, the modular microscope system can automatically adjust imaging parameters, provide high-precision imaging and data processing, significantly reduce human operation errors, and improve experimental efficiency. This automation function is particularly suitable for experiments that require long-term stable observation or large-scale sample analysis, not only ensuring the consistency of imaging, but also continuously outputting high-quality data, thereby effectively improving the reliability and repeatability of the experiment. Overall, the optical microscope imaging module is a more efficient and accurate experimental tool, making its application in scientific research more intelligent and more flexible.

[0092] The optical microscope imaging module of the present application has the advantage of significant modularity. The independence of the bright field imaging module and the fluorescence imaging module allows users to switch imaging modes flexibly according to experimental needs, and the modular design facilitates maintenance and upgrading. The optimized configuration of optical components, including multiple magnification semi-complex achromatic objectives, tube lenses and filters, ensures the accuracy and stability of imaging quality.

[0093] The fluorescence imaging module of the present application uses built-in UV, blue, green and other LED light sources and multiple fluorescence modules to meet the needs of different dyes and samples, while the dichroic mirror module ensures effective excitation and emission wavelength separation, optimizing the fluorescence imaging effect. The setting of aluminum alloy shell and high-efficiency cooling fan effectively reduces the temperature rise, ensuring the stability of long-time operation. These not only improve the imaging quality, but also ensure the durability and expandability of the equipment.

[0094] The optical microscope imaging module combines high performance with easy operation, demonstrating excellent adaptability in various research environments. By integrating advanced image enhancement algorithms, the microscope imaging module not only provides high-quality images but also significantly improves experimental efficiency and reduces human operation errors. These features enable the optical microscope imaging module to exhibit high reliability and automation levels in experiments requiring long-term stable observation, greatly improving the accuracy and continuity of research work.

[0095] Although the content of the present application has been described in detail through the above preferred embodiments, it should be recognized that the above description should not be considered as limiting the present application. After reading the above content, various modifications and alternatives of the present application will be apparent to those skilled in the art. Therefore, the scope of protection of the present application should be defined by the appended claims.

Claims

1. An optical microscope imaging module, characterized in that, The module comprises: a bright field imaging module and a bright field imaging module shell or a fluorescence imaging module and a fluorescence imaging module shell, the bright field imaging module is placed in the bright field imaging module shell, and the fluorescence imaging module is placed in the fluorescence imaging module shell; the bright field imaging module shell is composed of a bright field main body frame (1) and a bright field door (2); a round opening is arranged at the top of the bright field main body frame (1) and used for the extension of an objective lens (4); a square opening is arranged at the side of the bright field main body frame (1) close to the bottom and used for the connection of a data interface; a square support is arranged on the front end face of the bright field main body frame (1) and used for fixing the bright field door (2); the fluorescence imaging module shell is composed of a fluorescence main body frame (10) and a fluorescence door (11); a round opening is arranged at the top of the fluorescence main body frame (10) and used for the extension of an objective lens; square openings and circular holes are arranged at the side of the fluorescence main body frame (10) close to the bottom.

2. The optical microscope imaging module of claim 1, wherein, The bright field imaging module comprises: a bright field upper adapter (7), a bright field lower adapter (8), a filter drawer (5), an objective lens (4), a tube lens (6) and a CCD camera (9); the upper end of the bright field upper adapter (7) is screwed with the objective lens (4) through a threaded hole, and the lower end of the bright field upper adapter (7) is coaxially provided with an inner threaded hole and an outer threaded hole; the lower end of the bright field upper adapter (7) is connected with the tube lens (6) through the inner threaded hole; the lower end of the tube lens (6) is connected with the bright field lower adapter (8) through the outer threaded hole, and the lower end of the bright field lower adapter (8) is connected with the CCD camera (9); a groove is arranged on the side of the bright field upper adapter (7) and used for placing the filter drawer (5).

3. The optical microscope imaging module of claim 2, wherein, The fluorescence imaging module comprises: a fluorescence upper adapter (14), a fluorescence lower adapter (17), a light source shell (13), an objective lens (4), a tube lens (6), a dichroic mirror module (19), a dichroic mirror shell (15) and a CCD camera (9); a groove is arranged in the light source shell (13) and used for placing a heat dissipation module (18) and an LED light source; a threaded hole is arranged on the outer side of the light source shell (13) and used for fixing the fluorescence upper adapter (14); the upper end face of the fluorescence upper adapter (14) is connected with the objective lens (4) through a threaded hole, and the lower end of the fluorescence upper adapter (14) is connected with the tube lens (6); the lower end of the tube lens (6) is connected with the fluorescence lower adapter (17), and the lower end of the fluorescence lower adapter (17) is connected with the CCD camera (9); a groove is arranged in the fluorescence upper adapter (14) and used for fixing the light source shell (13), and a circular hole is arranged on the side of the fluorescence upper adapter (14) and used for the input of the LED light source.

4. The optical microscope imaging module of claim 3, wherein, The bicolour mirror shell (15) is fixed on one side of the fluorescence up adapter (14), the bicolour mirror shell (15) is composed of a bayonet (16), a bicolour mirror module (19) and a latch (20); the bicolour mirror module (19) is fixed in the bicolour mirror shell (15), and a groove and a square port are arranged on the outer side of the bicolour mirror shell (15); the bayonet (16) is provided with a square protrusion for fixing the latch (20); the latch (20) is provided with a square groove for fixing the bayonet (16).

5. The optical microscope imaging module of claim 3, wherein, The tube lens (6) is replaced by a 1X ordinary lens, and NA=0.

025.

6. The optical microscope imaging module of claim 3, wherein, The bright field up adapter (7), the bright field down adapter (8), the fluorescence up adapter (14), the fluorescence down adapter (17), the light source shell (13), the bicolour mirror shell (15), the bright field imaging module shell and the fluorescence imaging module shell are all made of aluminum alloy material.

7. The optical microscope imaging module of claim 3, wherein, The bicolour mirror module (19) is divided into a DAPI module, an FITC module and a TRITC module; the DAPI module has an excitation wavelength of 350-365 nm and an emission wavelength of 460-500 nm; the FITC module has an excitation wavelength of 475-490 nm and an emission wavelength of 510-560 nm; and the TRITC module has an excitation wavelength of 525-545 nm and an emission wavelength of 580-620 nm. The bicolour mirror module (19) is divided into a DAPI module, an FITC module and a TRITC module; the DAPI module has an excitation wavelength of 350-365 nm and an emission wavelength of 460-500 nm; the FITC module has an excitation wavelength of 475-490 nm and an emission wavelength of 510-560 nm; and the TRITC module has an excitation wavelength of 525-545 nm and an emission wavelength of 580-620 nm.

Images