Chromatographic device

Abstract

The utility model provides a chromatographic device. The chromatographic device comprises a chromatographic column and a receiving bottle, the number of sections of the chromatographic column is at least two; every two sections of the chromatographic column are fixed by a three-section hoop; a filter membrane is arranged between the receiving head of the receiving bottle and the chromatographic column; the filter membrane comprises a nylon filter membrane with the pore diameter of 0.1-1 mu m, a PTFE filter membrane with the pore diameter of 0.1-1 mu m or a UPE filter membrane with the pore diameter of 0.1-1 mu m. According to the utility model, the filter membrane is arranged between the receiving head of the receiving bottle and the chromatographic column, so that mechanical impurities and organic polymers in a mixture to be separated can be well removed, and the impurities are prevented from polluting a target object; the chromatography device can be used for positive-pressure column chromatography, negative-pressure column chromatography or normal-pressure column chromatography, is wide in application range and has wide-range popularization and application prospects.

Description

TECHNICAL FIELD

[0001] The utility model relates to industrial equipment technical field especially relates to a chromatography device. BACKGROUND

[0002] In the chemical industry, chromatography column is a common device for purifying organic mixtures. The chromatography column is filled with a specific adsorbent, different polar or non-polar solvents are used as eluent according to different diameter-height ratio, the mixed product to be separated is dissolved in organic solvent, and flows through the adsorbent from top to bottom. Because the components in the product are different, the adsorption capacity of the adsorbent is different, and the time required for each component to flow out of the chromatography column is also different. By receiving eluent in different time periods, the purpose of separating different components in the mixed product can be achieved.

[0003] In the separation process, in order to achieve different separation effects, different proportions of eluent are used to separate impurities, but there is still a problem of low separation and elution efficiency, and even poor separation effect.

[0004] CN221535703U discloses a chromatography column, which comprises a chromatography column body, a protective assembly is arranged outside the chromatography column body, a protective shell is slidably connected to the outside of the chromatography column body, an observation window perpendicular to the axis direction of the chromatography column body is formed in the outer wall of the protective shell, a fixed end cover is connected to the end of the protective shell to limit and constrain the chromatography column body, a sealing plate is slidably connected to the protective shell in a direction perpendicular to the observation window, and the sealing plate, the protective shell and the fixed end cover form a sealed shell for protecting the chromatography column body. A locking assembly is located on one side of the protective shell to lock the sliding connection direction of the sealing plate. By using the arrangement mode of the chromatography column body and the protective assembly, under the action of the locking assembly, the sealing plate can form a sealed shell with the protective shell and the fixed end cover to protect the chromatography column, so that the chromatography column is not damaged by collision when not in use, and the sealing plate can be disassembled when the chromatography column is in use.

[0005] CN209155185U discloses a detachable multi-layer chromatography column device, which comprises a column pipe and a column pipe sample loading assembly inserted into the upper end of the column body. The column pipe is composed of a plurality of sub-column pipes arranged vertically from top to bottom and a column tail. The upper and lower parts of the sub-column pipes and the upper part of the column tail are provided with flange edges protruding outward along the radial edge of the column body. Screws pass through the bolt holes in the flange edges to connect the two sub-column pipes and the column tail. The column pipe sample loading assembly comprises a feed pipe, a hollow rotary screw rod, a fixed nut part, a fixed plate and a feed cavity. The fixed plate and the fixed nut part located above the fixed plate are sleeved on the rotary screw rod. The fixed plate is placed on the flange edge of the uppermost sub-column body. The lower end of the rotary screw rod is connected to the feed cavity in the column pipe. The device has the characteristics of efficient and reasonable sample loading, detachable column body and column tail, and automatic operation.

[0006] CN213100923U discloses a column device for multi-layer chromatography layer, including first chromatographic column, sealable hopper, air pump, sealing structure and support frame, the top of the first chromatographic column one side is provided with sealable hopper, and the first chromatographic column below is provided with second chromatographic column, the top of the first chromatographic column one side of sealable hopper is fixed with mounting plate, and the top of mounting plate is installed with air pump, and the output end of air pump is fixed with conduit, the bottom of the second chromatographic column is provided with third chromatographic column, and the bottom of the third chromatographic column is provided with fourth chromatographic column, the bottom of the fourth chromatographic column is fixed with liquid outlet pipe at the center position, the connecting place of the first chromatographic column and the second chromatographic column, the second chromatographic column and the third chromatographic column, the third chromatographic column and the fourth chromatographic column is provided with sealing structure.

[0007] But the above-mentioned chromatographic column still has the problem of not being able to remove mechanical impurities and macromolecular polymers in oil products well. The utility model discloses a chromatographic device

[0008] In view of the problems in the prior art, the chromatographic device is provided, which can well remove mechanical impurities and organic polymers in the mixture to be separated by setting a filter membrane between the receiving head of the receiving bottle and the chromatographic column, thereby avoiding the pollution of the impurities to the target substance.

[0009] To achieve this purpose, the utility model adopts the following technical scheme:

[0010] The chromatographic device comprises a chromatographic column and a receiving bottle, and a specific filter membrane is arranged between the receiving head of the receiving bottle and the chromatographic column, so that mechanical impurities (carbon black and dust) and macromolecular polymers (polymer colloidal molecules with a molecular weight greater than 10) with a particle size of 0.1-30 microns can be efficiently removed, thereby avoiding the pollution of the impurities to the target substance.

[0011] The receiving head of the receiving bottle is provided with a filter membrane; the filter membrane comprises a nylon filter membrane with a pore size of 0.1-1 microns, a PTFE filter membrane with a pore size of 0.1-1 microns, or a UPE filter membrane with a pore size of 0.1-1 microns.

[0012] The chromatographic device comprises a chromatographic column and a receiving bottle, and a specific filter membrane is arranged between the receiving head of the receiving bottle and the chromatographic column, so that mechanical impurities (carbon black and dust) and macromolecular polymers (polymer colloidal molecules with a molecular weight greater than 10) with a particle size of 0.1-30 microns can be efficiently removed, thereby avoiding the pollution of the impurities to the target substance.

[0013] The chromatography apparatus described in this invention has at least two chromatography column segments, which can be increased as needed. Different substances are separated into different chromatography segments according to their separation effect. Then, the three-section clamps are selectively removed to separate the chromatography column before rinsing, improving rinsing effect and separation efficiency. In this invention, each chromatography column segment is fixed with a three-section clamp, which prevents uneven stress during installation that could lead to column breakage and facilitates column removal.

[0014] The filter membrane described in this invention includes a nylon filter membrane with a pore size of 0.1~1μm, a PTFE filter membrane with a pore size of 0.1~1μm, or a UPE filter membrane with a pore size of 0.1~1μm. For example, it can be 0.1μm, 0.2μm, 0.3μm, 0.5μm, 0.7μm, 0.9μm, or 1μm, but it is not limited to the listed values. Other unlisted values ​​within this range are also applicable.

[0015] Preferably, the wall thickness of the chromatography column is 3 to 5 mm, for example, it can be 3 mm, 3.3 mm, 3.5 mm, 4 mm, 4.2 mm, 4.5 mm or 5 mm, but it is not limited to the listed values. Other unlisted values ​​within this range are also applicable.

[0016] The chromatography column of this invention is mainly made of glass, and the wall thickness of the chromatography column is 3~5mm. Different types of column chromatography methods such as pressurized, atmospheric, or negative pressure can be selected. This overcomes the resistance of different column combination heights and different adsorbent fineness to the product solution and eluent, and avoids the problem of excessive column chromatography pressure causing column breakage.

[0017] Preferably, a sand core plate is provided at the bottom of each section of the chromatography column to effectively prevent damage to the adsorbent during the dismantling process and to avoid affecting the secondary column chromatography effect.

[0018] Preferably, the chromatography column is filled with an adsorbent. A suitable adsorbent can be selected based on the actual condition of the mixture to be separated, and the adsorbent should be level with the top edge of the chromatography column.

[0019] Preferably, the uppermost chromatography column is connected to the chromatography column cap.

[0020] Preferably, the chromatography column cap is provided with an eluent inlet, which can pressurize the interior of the column chromatography.

[0021] Preferably, the receiving head of the receiving bottle is provided with a sand core plate at its upper end.

[0022] Preferably, the receiving bottle is provided with a vacuum port to facilitate vacuuming or emptying the receiving bottle.

[0023] Preferably, the thickness of the sand core plate is 1 to 10 mm, for example, it can be 1 mm, 2 mm, 3 mm, 5 mm, 8 mm, 9 mm or 10 mm, but it is not limited to the listed values. Other unlisted values ​​within this range are also applicable.

[0024] Preferably, the aperture of the sand core plate is 100 to 500 mesh, for example, it can be 100 mesh, 120 mesh, 150 mesh, 200 mesh, 300 mesh, 400 mesh or 500 mesh, etc., but it is not limited to the listed values. Other unlisted values ​​within this range are also applicable.

[0025] Preferably, the receiving head of the receiving bottle is fixed to the chromatography column using a three-section clamp.

[0026] Preferably, the three-section clamp includes a first locking part, a second locking part, and a third locking part connected in sequence.

[0027] Preferably, the first locking part and the second locking part are hinged via a pin shaft.

[0028] Preferably, the third locking part is hinged to the second locking part via a pin shaft.

[0029] Preferably, the first locking part and the third locking part are connected by bolts.

[0030] The method of using the chromatography apparatus of this utility model includes the following steps:

[0031] (1) Install the adsorbent: Install the receiving head and receiving bottle of the receiving bottle, and add the corresponding adsorbent to the chromatography column so that the adsorbent is horizontal with the top edge of the chromatography column. Draw negative pressure from the vacuum port of the receiving bottle to compact the adsorbent in the chromatography column, ensuring that there are no gaps at the connection between the adsorbent in the chromatography column and the upper chromatography column. Install the adsorbent for each section of the chromatography column separately, and fix the chromatography columns together with three-section clamps. Increase the height of the chromatography column to the required number of sections according to the number of separation layers required for the target substance.

[0032] (2) Perform chromatography separation: After adding the mixture to be separated to the uppermost section of the chromatography column, add chromatography column eluent to perform chromatography separation; different column chromatography methods can be selected.

[0033] 2.1 Pressure-driven column chromatography: The column height is increased to the required number of chromatography layers according to the target substance. The top layer is left blank. No adsorbent is added to the chromatography column. Only the mixture to be separated is added. The chromatography column is sealed and the eluent is pumped into the chromatography column under pressure. The pressure inside the column is within 0.05MPa~0.1MPa.

[0034] 2.2 Atmospheric pressure column chromatography: The column height is increased to the required number of chromatography layers according to the target substance. The top layer of the column is left without adsorbent, only the mixture to be separated is added, and the eluent is directly added to the chromatography column for gravity washing.

[0035] 2.3 Negative pressure column chromatography: The column height is increased to the required number of chromatography layers according to the target substance. The top layer of the column is left without adsorbent, only the mixture to be separated is added. The eluent is added to the top layer of the column and the eluent is eluted through the vacuum port of the receiving bottle under negative pressure. The pressure inside the column is within -0.05MPa to -0.1MPa.

[0036] When separating a single target analyte, different components are separated by rinsing with an eluent. After identifying the target analyte column segment, the three-section clamping column segment can be directly removed. Then, the target analyte column segment is connected to a new receiving bottle and receiving head for rinsing, and the target analyte is obtained quickly.

[0037] When separating multiple targets, the three-section clamp can be removed simultaneously to remove multiple target chromatography columns based on the separation effect of multiple components. Then, the target column segments are connected to new receiving bottles and receiving heads and eluted to quickly obtain each target.

[0038] Compared with the prior art, the present invention has at least the following beneficial effects:

[0039] (1) The chromatography device provided by this utility model has a reasonable structural design. By setting a filter membrane between the receiving head of the receiving bottle and the chromatography column, mechanical impurities and macromolecular polymers are removed, thus avoiding contamination of the target substance by these impurities. Moreover, it can also test the degree of contamination of mechanical impurities or organic polymers in the mixture to be separated.

[0040] (2) Each section of the chromatography column in the chromatography device provided by this utility model is fixed with a three-section clamp to prevent uneven stress during installation, which could lead to uneven stress and breakage of the chromatography column, and facilitates the removal of the chromatography column.

[0041] (3) The present invention can perform different types of column chromatography and has a wide range of applications. Attached Figure Description

[0042] Figure 1 This is a schematic diagram of the overall structure of the chromatography apparatus provided by this utility model.

[0043] Figure 2 This is a schematic diagram of the three-section clamp in the chromatography device provided by this utility model.

[0044] In the diagram: 1-Eluent inlet; 2-Column cap; 3-Column eluent; 4-Mix to be separated; 5-Column; 6-Adsorbent; 7-Receiver head; 8-Vacuum port; 9-Receiver bottle; 10-Filter membrane; 11-Sand core plate; 12-Three-section clamp; 13-First clamping part; 14-Second clamping part; 15-Third clamping part; 16-Bolt. Detailed Implementation

[0045] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0046] This utility model provides a chromatography apparatus, the overall structure of which is shown in the schematic diagram below. Figure 1 As shown.

[0047] The chromatography apparatus includes a chromatography column 5 and a receiving bottle 9; the chromatography column 5 has 3 segments; each segment of the chromatography column 5 is fixed together by a three-section clamp 12;

[0048] A filter membrane 10 is disposed between the receiving head 7 of the receiving bottle 9 and the chromatography column 5; the filter membrane 10 includes a nylon filter membrane with a pore size of 0.1~1μm, a PTFE filter membrane with a pore size of 0.1~1μm, or a UPE filter membrane with a pore size of 0.1~1μm.

[0049] The wall thickness of the chromatography column 5 is 3~5mm.

[0050] Each section of the chromatography column 5 is equipped with a sand core plate 11 at its bottom.

[0051] The chromatography column 5 is filled with adsorbent 6.

[0052] The topmost chromatography column 5 is connected to the chromatography column cap 2.

[0053] The chromatography column cap 2 is provided with an eluent inlet 1.

[0054] A sand core plate 11 is provided at the upper end of the receiving head 7 of the receiving bottle 9;

[0055] The receiving bottle 9 is provided with a vacuum port 8.

[0056] The thickness of the sand core plate 11 is 1~10mm;

[0057] The aperture of the sand core plate 11 is 100~500 mesh.

[0058] The receiving head 7 of the receiving bottle 9 is fixed to the chromatography column 5 by a three-section clamp 12.

[0059] The three-section clamp 12 includes a first locking part 13, a second locking part 14, and a third locking part 15 connected in sequence; the structural diagram is shown below. Figure 2 As shown.

[0060] The first locking part 13 and the second locking part 14 are hinged via a pin shaft;

[0061] The third locking part 15 is hinged to the second locking part 14 via a pivot pin.

[0062] The first locking part 13 and the third locking part 15 are connected by bolts 16.

[0063] The method of using the above-mentioned chromatography apparatus includes the following steps:

[0064] (1) Install the adsorbent: After installing the receiving head 7 and receiving bottle 9, add the corresponding adsorbent 6 to the chromatography column 5, making the adsorbent 6 horizontal with the upper edge of the chromatography column 5. Draw negative pressure from the vacuum port 8 of the receiving bottle 9 to compact the adsorbent 6 in the chromatography column 5, ensuring that there are no gaps at the connection between the adsorbent 6 in the chromatography column 5 and the upper chromatography column 5. Install the adsorbent 6 separately for each section of the chromatography column 5, and fix the chromatography columns 5 together with the three-section clamps 12. Increase the height of the chromatography column 5 to the required number of sections according to the number of separation layers required for the target substance.

[0065] (2) Perform chromatography separation: After adding the mixture 4 to be separated to the uppermost chromatography column 5, add the chromatography column eluent 3 and select different column chromatography methods.

[0066] The present invention will now be described in further detail. However, the examples described below are merely simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be determined by the claims.

[0067] It should be understood that in the description of this utility model, the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used solely for the convenience of describing this utility model and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0068] It should be noted that, in the description of this utility model, unless otherwise explicitly specified and limited, the terms "set," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0069] Example 1

[0070] This embodiment provides a chromatography apparatus, which includes a chromatography column and a receiving bottle; the chromatography column has 7 segments, named from bottom to top as segment 1 to segment 7, wherein segment 7 is not filled with adsorbent, and the remaining 6 segments are filled with adsorbent; each segment of the chromatography column is fixed together with a three-section clamp;

[0071] A filter membrane is disposed between the receiving head of the receiving bottle and the chromatography column; the filter membrane is a nylon filter membrane with a pore size of 1 μm.

[0072] The column has a wall thickness of 3 mm.

[0073] Each chromatography column has a sand core plate at the bottom.

[0074] The topmost chromatography column is connected to the column cap. The column cap is equipped with an eluent inlet.

[0075] The receiving head of the receiving bottle is provided with a sand core plate; the receiving bottle is provided with a vacuum port.

[0076] The thickness of the sand core plate is 2mm; the aperture of the sand core plate is 500 mesh.

[0077] The receiving head of the receiving bottle is fixed to the chromatography column using a three-section clamp.

[0078] The three-section clamp includes a first locking part, a second locking part, and a third locking part connected in sequence; the first locking part and the second locking part are hinged via a pin; the third locking part and the second locking part are hinged via a pin; the first locking part and the third locking part are connected by bolts.

[0079] The method of using the chromatography apparatus described in this embodiment includes the following steps:

[0080] The mixture to be separated (substances A, B, and C, where substance B is the target analyte) is added to the 7th stage of the chromatography column, and the column is sealed tightly. The eluent is forced into the column through the eluent inlet under pressure for column chromatography. The column pressure is maintained between 0.05 MPa and 0.1 MPa.

[0081] The eluent separates substance A to section 2 of the chromatography column, while substance B goes to section 4. When substance C is still in section 7, the eluent inlet is closed. After 3 minutes, the three-section clamp connecting section 3 and section 4 of the chromatography column is removed. The filter membrane is placed on the receiving head of a pre-installed receiving bottle, and section 4 of the chromatography column containing substance B is connected to the receiving head using the three-section clamp. The eluent is then added to section 7 of the chromatography column for elution, directly yielding the target substance B.

[0082] Example 2

[0083] This embodiment provides a chromatography apparatus, which includes a chromatography column and a receiving bottle; the chromatography column has 7 segments, named from bottom to top as segment 1 to segment 7, wherein segment 7 is not filled with adsorbent, and the remaining 6 segments are filled with adsorbent; each segment of the chromatography column is fixed together with a three-section clamp;

[0084] A filter membrane is disposed between the receiving head of the receiving bottle and the chromatography column; the filter membrane is a PTFE filter membrane with a pore size of 0.1 μm.

[0085] The column has a wall thickness of 4 mm.

[0086] Each chromatography column has a sand core plate at the bottom.

[0087] The topmost chromatography column is connected to the column cap. The column cap is equipped with an eluent inlet.

[0088] The receiving head of the receiving bottle is provided with a sand core plate; the receiving bottle is provided with a vacuum port.

[0089] The thickness of the sand core plate is 1 mm; the aperture of the sand core plate is 100 mesh.

[0090] The receiving head of the receiving bottle is fixed to the chromatography column using a three-section clamp.

[0091] The three-section clamp includes a first locking part, a second locking part, and a third locking part connected in sequence; the first locking part and the second locking part are hinged via a pin; the third locking part and the second locking part are hinged via a pin; the first locking part and the third locking part are connected by bolts.

[0092] The method of using the chromatography apparatus described in this embodiment includes the following steps:

[0093] The mixture to be separated (substances A, B, and C, where substance B is the target analyte) is added to the 7th stage of the chromatography column, and the column is sealed tightly. The eluent enters the column through the eluent inlet for column chromatography. A vacuum port is connected via a vacuum tube, and the vacuum pump is turned on, maintaining the pressure inside the receiving flask within -0.05 MPa to -0.1 MPa.

[0094] The eluent separates substance A to section 2 of the chromatography column, while substance B goes to section 4. When substance C is still in section 7, the vacuum pump and eluent inlet are turned off. After 3 minutes, the three-section clamp connecting section 3 and section 4 of the chromatography column is removed. The filter membrane is placed on the receiving head of the prepared receiving bottle, and section 4 of the chromatography column containing substance B is connected to the receiving head using the three-section clamp. The vacuum pump is then turned on again, and the eluent is added to section 7 of the chromatography column for elution, directly obtaining the target substance B.

[0095] Example 3

[0096] This embodiment provides a chromatography apparatus, which includes a chromatography column and a receiving bottle; the chromatography column has 7 segments, named from bottom to top as segment 1 to segment 7, wherein segment 7 is not filled with adsorbent, and the remaining 6 segments are filled with adsorbent; each segment of the chromatography column is fixed together with a three-section clamp;

[0097] A filter membrane is disposed between the receiving head of the receiving bottle and the chromatography column; the filter membrane is a UPE filter membrane with a pore size of 0.5 μm.

[0098] The column has a wall thickness of 5 mm.

[0099] Each chromatography column has a sand core plate at the bottom.

[0100] The topmost chromatography column is connected to the column cap. The column cap is equipped with an eluent inlet.

[0101] The receiving head of the receiving bottle is provided with a sand core plate; the receiving bottle is provided with a vacuum port.

[0102] The thickness of the sand core plate is 10mm; the aperture of the sand core plate is 300 mesh.

[0103] The receiving head of the receiving bottle is fixed to the chromatography column using a three-section clamp.

[0104] The three-section clamp includes a first locking part, a second locking part, and a third locking part connected in sequence; the first locking part and the second locking part are hinged via a pin; the third locking part and the second locking part are hinged via a pin; the first locking part and the third locking part are connected by bolts.

[0105] The method of using the chromatography apparatus described in this embodiment includes the following steps:

[0106] The mixture to be separated (substance A, substance B and substance C, where substance B is the target substance) is added into the 7th section of the chromatography column, and the eluent is added into the chromatography column through the eluent inlet for column chromatography.

[0107] The eluent separates substance A to section 2 of the chromatography column, while substance B goes to section 4. When substance C is still in section 7, immediately remove the three-section clamp connecting section 3 and section 4 of the chromatography column. Place the filter membrane on the receiving head of the prepared receiving bottle, and connect section 4 of the chromatography column containing substance B to the receiving head using the three-section clamp. Then, continue adding eluent to section 7 of the chromatography column for elution, directly obtaining the target substance B.

[0108] The solutions of substance B obtained in Examples 1-3 were filtered through a filter membrane with the same volume of solution. The membrane quality diagram showed that substance B contained no mechanical impurities or organic polymers.

[0109] Comparative Example 1

[0110] This comparative example provides a chromatography column device, which is an integral chromatography column. The chromatography device includes a chromatography column and a receiving bottle. The chromatography column has only one section, and the column is filled with an adsorbent of the same height as in Example 1. The top layer of the adsorbent is the mixture to be separated. The seal is integral with the chromatography column and the receiving head.

[0111] The comparative chromatography column cap is equipped with an eluent inlet.

[0112] The comparative example has a receiving head equipped with a sand core plate; the comparative example has a receiving bottle equipped with a vacuum port.

[0113] The thickness of the sand core plate at the top of the comparative receiver is 2mm; the aperture of the comparative sand core plate is 500 mesh.

[0114] The method of using the chromatography apparatus described in this comparative example includes the following steps:

[0115] The mixture to be separated (substances A, B, and C, where substance B is the target analyte) is added into the chromatography column, and the column is sealed tightly. The eluent is forced into the column through the eluent inlet under pressure for column chromatography; the column pressure is maintained between 0.05 MPa and 0.1 MPa, yielding the target analyte, substance B.

[0116] The substance B solution obtained in Comparative Example 1 was filtered through a membrane using the same volume of solution. The membrane quality graph showed that the membrane was dirty, indicating that substance B contained mechanical impurities and organic polymers, and its purity was low.

[0117] Comparative Example 2

[0118] This comparative example provides a chromatography apparatus, which is an integral chromatography column. The chromatography apparatus includes a chromatography column and a receiving bottle. The chromatography column has only one section, and the column is filled with an adsorbent of the same height as in the example. The top layer of the adsorbent is the mixture to be separated. The seal is integral with the chromatography column and the receiving head.

[0119] The comparative chromatography column cap is equipped with an eluent inlet.

[0120] The comparative example has a receiving head equipped with a sand core plate; the comparative example has a receiving bottle equipped with a vacuum port.

[0121] The thickness of the sand core plate at the top of the comparative receiver is 2mm; the aperture of the comparative sand core plate is 500 mesh.

[0122] The method of using the chromatography apparatus described in this comparative example includes the following steps:

[0123] The mixture to be separated (substances A, B, and C, where substance B is the target analyte) is added to the chromatography column, and the column is sealed tightly. The eluent enters the column through the eluent inlet for column chromatography. A vacuum port is connected via a vacuum tube, and the vacuum pump is turned on. The pressure inside the receiving flask is maintained within -0.05 MPa to -0.1 MPa to obtain the target analyte, substance B.

[0124] Comparative Example 3

[0125] This comparative example provides a chromatography apparatus, which is an integral chromatography column. The chromatography apparatus includes a chromatography column and a receiving bottle. The chromatography column has only one section, and the column is filled with an adsorbent of the same height as in the example. The top layer of the adsorbent is the mixture to be separated. The seal is integral with the chromatography column and the receiving head.

[0126] The comparative chromatography column cap is equipped with an eluent inlet.

[0127] In the comparative example, the receiving head is equipped with a sand core plate.

[0128] The thickness of the sand core plate at the top of the comparative receiver is 2mm; the aperture of the comparative sand core plate is 500 mesh.

[0129] The method of using the chromatography apparatus described in this comparative example includes the following steps:

[0130] The mixture to be separated (substance A, substance B, and substance C, where substance B is the target analyte) is added into the chromatography column. The eluent is added into the chromatography column through the eluent inlet for column chromatography to obtain the target analyte B.

[0131] The time to obtain the target analytes and the purity of the target analytes after column chromatography of Examples 1-3 and Comparative Examples 1-3 under positive pressure, negative pressure and normal pressure are shown in Table 1.

[0132]

[0133] As can be seen from Table 1:

[0134] (1) As can be seen from Examples 1 to 3, the chromatography apparatus provided by this utility model can quickly separate the target substance B under positive pressure, negative pressure and normal pressure, and the purity of the target substance B is high, at 99%;

[0135] (3) Comparative Examples 1 to 3 show that using the existing chromatography apparatus to separate the target substance B under positive pressure, negative pressure and normal pressure takes a long time; and the purity of the target substance B is low. In Comparative Example 3, the normal pressure column chromatography, although the purity of the target substance B is 99%, the separation time is long, which is 10 hours.

[0136] The composition of substance B obtained in Examples 1-3 and Comparative Examples 1-3 is shown in Table 2.

[0137]

[0138] As can be seen from Table 2, the contents of substances A and C in Examples 1-3 are all below 1%, while the contents of target substance B are relatively high; the contents of substances A and C in Comparative Example 1 are relatively high, while the contents of target substance B are 98%; the contents of target substance B in Comparative Example 3 are 99%.

[0139] The applicant declares that the detailed structural features of this utility model are illustrated through the above embodiments, but this utility model is not limited to the above detailed structural features, that is, it does not mean that this utility model must rely on the above detailed structural features to be implemented. Those skilled in the art should understand that any improvements to this utility model, equivalent substitutions of selected components, additions of auxiliary components, and selection of specific methods, etc., all fall within the protection and disclosure scope of this utility model.

[0140] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

Claims

1. A chromatography apparatus, characterized in that, The chromatography apparatus includes a chromatography column and a receiving bottle; the chromatography column has at least two segments; each segment of the chromatography column is fixed together by a three-section clamp. A filter membrane is disposed between the receiving head of the receiving bottle and the chromatography column; the filter membrane includes a nylon filter membrane with a pore size of 0.1~1μm, a PTFE filter membrane with a pore size of 0.1~1μm, or a UPE filter membrane with a pore size of 0.1~1μm.

2. The chromatography apparatus according to claim 1, characterized in that, The wall thickness of the chromatography column is 3~5mm.

3. The chromatography apparatus according to claim 1, characterized in that, Each chromatography column has a sand core plate at the bottom.

4. The chromatography apparatus according to claim 1, characterized in that, The chromatography column is filled with an adsorbent.

5. The chromatography apparatus according to claim 1, characterized in that, The top layer of the chromatography column is connected to the column cap.

6. The chromatography apparatus according to claim 5, characterized in that, The chromatography column cap is equipped with an eluent inlet.

7. The chromatography apparatus according to claim 1, characterized in that, The receiving head of the receiving bottle is provided with a sand core plate at its upper end; The receiving bottle is equipped with a vacuum port.

8. The chromatography apparatus according to claim 7, characterized in that, The thickness of the sand core plate is 1~10mm; The pore size of the sand core plate is 100~500 mesh.

9. The chromatography apparatus according to claim 1, characterized in that, The receiving head of the receiving bottle is fixed to the chromatography column with a three-section clamp.

10. The chromatography apparatus according to claim 1 or 9, characterized in that, The three-section clamp includes a first locking part, a second locking part, and a third locking part connected in sequence; The first locking part and the second locking part are hinged together by a pin shaft; The third locking part is hinged to the second locking part via a pin shaft; The first locking part and the third locking part are connected by bolts.

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