Nucleic acid extraction device adopting paramagnetic particle method

Abstract

The utility model discloses a nucleic acid extraction device adopting a paramagnetic particle method. The nucleic acid extraction device comprises a transfer pipette, a jacketed pipe and a magnet sleeve, wherein the jacketed pipe is sleeved outside the transfer pipette; the magnet sleeve is arranged in a gap between the transfer pipette and the jacketed pipe; wherein a magnetic isolation coating is arranged in the middle of the outer wall of the transfer pipette; a plurality of preformed holes are formed in the top of the jacketed pipe, and the top of the magnet sleeve is adjusted to ascend and descend through a pull rope; and the end part of the pull rope penetrates through the preformed hole and is connected with the pull ring. According to the utility model, the pull rope drives the magnet sleeve to rise along the inner wall of the jacketed pipe until the magnet sleeve crosses over the magnetic isolation coating area on the outer wall of the transfer pipette; when the magnet sleeve ascends and passes through the area of the magnetism isolating coating, the magnetic attraction force of the magnet sleeve to the magnetic beads is isolated by the magnetism isolating coating, so that the magnetic beads fall into the pipette, and the magnetic beads in the pipette are discharged by extruding the balloon; therefore, the purpose of quickly adjusting the magnetic suction to suck or discharge the transfer pipette is achieved, and the operation efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of biological nucleic acid extraction technology, and in particular to a magnetic bead-based nucleic acid extraction device. Background Technology

[0002] Nucleic acids are currently the carriers of genetic information and the most important biological information molecules, making them a primary focus of molecular biology research. Therefore, nucleic acid extraction is the most important and fundamental operation in molecular biology experimental techniques. With the rapid development of molecular biology techniques, nucleic acid research and analysis are increasingly being promoted and applied in fields such as clinical diagnosis, environmental monitoring, and food safety testing, playing a significant role.

[0003] When extracting nucleic acids using magnetic beads, conventional pipettes require external magnets to magnetically position the beads, which affects the efficiency of the pipette operation. Utility Model Content

[0004] The purpose of this invention is to provide a magnetic bead-based nucleic acid extraction device that solves the problem that magnetic beads in the pipette are not easy to be magnetically positioned in conventional magnetic bead-based operations.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0006] This utility model provides a magnetic bead-based nucleic acid extraction device, including a pipette, a jacket sleeve sleeved outside the pipette, and a magnetic sleeve disposed in the gap between the pipette and the jacket sleeve;

[0007] A magnetic shielding coating is provided in the middle of the outer wall of the pipette; the magnet sleeve is less than 1 / 2 the length of the jacket tube;

[0008] Multiple pre-drilled holes are provided at the top of the jacket tube, and the top of the magnet sleeve is adjusted for height by a pull rope; the end of the pull rope passes through the pre-drilled holes and is connected to the pull ring.

[0009] Furthermore, the inner diameter of the magnet sleeve is larger than the outer diameter of the pipette and smaller than the inner diameter of the jacket tube; the distance between the pipette and the jacket tube is less than twice the wall thickness of the magnet sleeve.

[0010] Furthermore, the jacketed tube is a plastic tube that is sealed to the outside of the pipette.

[0011] Furthermore, at least three connectors are provided on the top of the magnet sleeve, and a pull rope is connected to each connector, with the upper end of the pull rope passing through the reserved hole.

[0012] Furthermore, the lower end of the pipette is connected to an aspiration tip, and the upper end of the pipette is connected to a balloon.

[0013] Compared with the prior art, the beneficial technical effects of this utility model are as follows:

[0014] In this invention, a pull rope drives the magnet sleeve to rise along the inner wall of the jacket tube until it passes the magnetic shielding coating area on the outer wall of the pipette. When the magnet sleeve rises past the magnetic shielding coating area, the magnetic attraction of the magnet sleeve to the magnetic bead is isolated by the magnetic shielding coating, causing the magnetic bead to fall into the inside of the pipette. The magnetic bead is then expelled from the pipette by squeezing the balloon. This allows for rapid adjustment of the magnetic attraction to or from the pipette, improving operational efficiency. Attached Figure Description

[0015] The present invention will be further described below with reference to the accompanying drawings.

[0016] Figure 1 This is a schematic diagram of the main structure of the magnetic bead-based nucleic acid extraction device of this utility model;

[0017] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0018] Figure 3 This is a schematic diagram of the external pull ring installation of the magnetic bead-based nucleic acid extraction device of this utility model;

[0019] Figure 4 for Figure 3 Schematic diagram of the BB section.

[0020] Explanation of reference numerals in the attached drawings: 100, pipette; 200, jacketed tube; 300, magnet sleeve; 301, pull rope; 3011, connector; 302, pull ring; 400, magnetic shielding coating; 500, magnetic bead. Detailed Implementation

[0021] like Figure 1 As shown, this embodiment discloses a magnetic bead-based nucleic acid extraction device, including a pipette 100, a jacketed tube 200 sleeved outside the pipette 100, and a magnetic sleeve 300 installed in the gap between the pipette 100 and the jacketed tube 200; wherein the lower end of the pipette 100 is connected to a suction tip, and the upper end of the pipette 100 is connected to a balloon; specifically, as Figure 1 and Figure 2 As shown, the magnet sleeve 300 is sleeved on the outside of the pipette 100, wherein the magnet sleeve 300 is used to adsorb magnetic beads present in the pipette 100.

[0022] In this embodiment, a magnetic shielding coating 400 is coated on the middle part of the outer wall of the pipette 100; the magnet sleeve 300 is less than 1 / 2 the length of the jacket tube 200; when the magnet sleeve 300 is raised, after the magnet sleeve 300 passes through the magnetic shielding coating 400, the magnetic adsorption of the magnet sleeve 300 on the magnetic bead is blocked.

[0023] Multiple pre-drilled holes are provided at the top of the jacket tube 200, and the top of the magnet sleeve 300 is adjusted for height via a pull rope 301; for example Figure 3 As shown, the end of the pull rope 301 passes through the reserved hole and is connected to the pull ring 302; wherein the pull rope 301 is used to raise and lower the magnet sleeve 300, thereby driving the magnet sleeve 300 to rise and fall relative to the magnetic shielding coating 400.

[0024] like Figure 4 As shown, in this embodiment, the inner diameter of the magnet sleeve 300 is larger than the outer diameter of the pipette 100 and smaller than the inner diameter of the jacket tube 200; in specific implementation, the distance between the pipette 100 and the jacket tube 200 is less than twice the wall thickness of the magnet sleeve 300; thereby preventing the magnet sleeve 300 from scratching the magnetic shielding coating 400 during the lifting and lowering process.

[0025] In this embodiment, the jacket tube 200 is a plastic tube that is sealed and installed outside the pipette 100; specifically, the bottom of the jacket tube 200 is a plastic annular bottom surface that can contact the end face of the magnet sleeve 300.

[0026] In this embodiment, three connectors 3011 are installed on the top of the magnet sleeve 300, and a pull rope 301 is connected to each connector 3011. The upper end of the pull rope 301 passes through the reserved hole. When applicable, the magnet sleeve 300 can be pulled up by directly pulling the pull rope 301 to adjust the specific position.

[0027] In this embodiment, when used:

[0028] During normal use, the magnet sleeve 300 is located at the bottom of the jacket tube 200 under the action of gravity;

[0029] First, adjust the solution with the adsorption of the magnetic beads 500 by the pipette 100; then, as follows Figure 2As shown, the magnetic beads 500 are all attracted to the inner arm of the pipette 100 by the magnetic attraction of the magnetic sleeve 300. When it is necessary to release and adjust the magnetic beads 500 in the pipette 100, the magnetic sleeve 300 is driven to rise along the inner wall of the jacket tube 200 by pulling down the pull ring 302 and the pull rope 301 until it passes through the area of ​​the magnetic shielding coating 400 on the outer wall of the pipette 100. When the magnetic sleeve 300 rises through the area of ​​the magnetic shielding coating 400, the magnetic attraction of the magnetic sleeve 300 to the magnetic beads is isolated by the magnetic shielding coating 400, so that the magnetic beads 500 fall into the interior of the pipette 100. The magnetic beads 500 are discharged from the pipette 100 by squeezing the balloon.

[0030] The above embodiments are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Any modifications and improvements made to the technical solutions of the present invention by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A magnetic bead-based nucleic acid extraction device, characterized in that: It includes a pipette (100), a jacket tube (200) sleeved outside the pipette (100), and a magnetic sleeve (300) disposed in the gap between the pipette (100) and the jacket tube (200). A magnetic shielding coating (400) is provided in the middle of the outer wall of the pipette (100); the magnet sleeve (300) is less than 1 / 2 the length of the jacket tube (200); Multiple pre-drilled holes are provided at the top of the jacket tube (200), and the top of the magnet sleeve (300) is adjusted for lifting and lowering by a pull rope (301); the end of the pull rope (301) passes through the pre-drilled holes and is connected to the pull ring (302).

2. The magnetic bead-based nucleic acid extraction device according to claim 1, characterized in that: The inner diameter of the magnet sleeve (300) is larger than the outer diameter of the pipette (100) and smaller than the inner diameter of the jacket tube (200); the distance between the pipette (100) and the jacket tube (200) is less than twice the wall thickness of the magnet sleeve (300).

3. The magnetic bead-based nucleic acid extraction device according to claim 2, characterized in that: The jacket tube (200) is a plastic tube that is sealed to the outside of the pipette (100).

4. The magnetic bead-based nucleic acid extraction device according to claim 3, characterized in that: At least three connectors (3011) are provided on the top of the magnet sleeve (300), and a pull rope (301) is connected to each connector (3011), with the upper end of the pull rope (301) passing through the reserved hole.

5. The magnetic bead-based nucleic acid extraction device according to claim 4, characterized in that: The lower end of the pipette (100) is connected to an aspiration tip, and the upper end of the pipette (100) is connected to a balloon.

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