Automatic workstation
By integrating refrigeration modules, liquid separation and balancing equipment, and other equipment into an automated workstation, the problem of low efficiency in the pre-crushing steps in existing technologies has been solved. The workstation automates weighing, balancing, and resuspension, improving experimental efficiency and safety while reducing equipment maintenance costs and space requirements.
Patent Information
- Application Number
- CN202520157229.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-22
- Publication Date
- 2026-02-06
- Estimated Expiration
- 2035-01-22
AI Technical Summary
In existing automated operating equipment, the steps before crushing are extremely inefficient, and the integration between various processes is low, resulting in a large number of sample transfers between different devices, which reduces the level of automation and experimental efficiency.
An automated workstation was designed, integrating a refrigeration module, liquid dispensing and balancing equipment, centrifugation equipment, capping equipment, cell disruption equipment, and liquid pouring equipment. A robotic arm transfers centrifuge bottles between these devices, and weighing, balancing, and resuspension are automated using a weighing sensor and a liquid aspiration mechanism, reducing equipment dependence and improving experimental efficiency.
Through integrated design and automated operation, the efficiency and safety of experiments are improved, equipment maintenance costs and space occupation are reduced, the risk of sample loss and cross-contamination is reduced, and the accuracy and reliability of experimental results are ensured.
Smart Images
Figure CN223879717U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of experimental operation technology, specifically to automated workstations. Background Technology
[0002] In existing technology, automated operating equipment includes a base, with a robotic arm mounted on the upper surface of the base. Surrounding the robotic arm on the upper surface of the base are a cell disruptor, a horizontal rotor centrifuge, a high-speed angle rotor centrifuge, a cap opener, a liquid pourer, an electronic scale, and a mixer. In specific operation, the dispensing instrument dispenses the culture medium into 12 50mL centrifuge bottles, then caps each bottle individually. These 12 centrifuge bottles are then sent to the horizontal rotor centrifuge for centrifugation. After centrifugation, each bottle is capped, and the supernatant is discarded. A resuspension is then added to the first centrifuge bottle, and the resuspension is poured into the second centrifuge bottle, with each subsequent bottle containing a higher concentration of bacteria, and so on, until the 12th centrifuge bottle has resuspended all the bacteria. This 12th centrifuge bottle is then sent to disrupt the cells, and the bottle containing the disrupted cells is then sent to high-speed centrifugation. Finally, the supernatant containing the target protein is obtained by pouring. It can be seen that the steps before crushing are extremely inefficient, and the integration between different processes is low. Each process is relatively independent, resulting in a large number of sample transfers between different devices, which reduces the level of automation and experimental efficiency. Utility Model Content
[0003] In view of this, the present invention provides an automated workstation to solve the problem that the steps before crushing are extremely inefficient and the integration between different processes is low in experimental operations. Each process is relatively independent, resulting in a large number of sample transfers between different devices, which reduces the level of automation and experimental efficiency.
[0004] This utility model provides an automated workstation, comprising:
[0005] Base;
[0006] A refrigeration module, disposed on the base, includes a refrigeration slot for storing centrifuge bottles;
[0007] The liquid separation and balancing device is set on the base and includes a weighing tank that corresponds to the specifications of the refrigeration tank and has a weighing sensor at the bottom, and a robotic arm set next to the weighing tank and equipped with a liquid suction mechanism. The liquid suction mechanism is used to balance and resuspend the target centrifuge bottle.
[0008] Centrifuge equipment, lid-opening equipment, cell disruption equipment, and liquid pouring equipment are respectively mounted on the base;
[0009] A robotic arm is used to transfer centrifuge bottles between the refrigeration module, the liquid dispensing and balancing device, the centrifugation device, the capping device, the cell disruption device, and the liquid pouring device.
[0010] Beneficial effects: By installing weighing sensors in the weighing tank, centrifuge bottles placed in the weighing tank provide storage space for the bottles and enable automatic weighing. Based on the weighing data, the liquid suction mechanism of the liquid balancing device is used to balance the centrifuge bottles, ensuring that the weight of the centrifuge bottles placed in the centrifuge is consistent, thereby ensuring the balance during the centrifugation process. This is crucial for improving centrifugation efficiency and separation effect.
[0011] Furthermore, the aspiration mechanism can add resuspension to the concentrate obtained after centrifugation in the previous step and repeatedly aspirate to mix it, ensuring the concentrate is resuspended in the resuspension. Thus, weighing, balancing, and resuspension are all performed on the liquid-liquid balancing device, eliminating the need for three separate devices in the past. This integration of multiple processes improves efficiency before crushing, enhances the automation level of the entire workstation, and increases experimental efficiency. Automated operation reduces the number of sample transfers between different devices, lowering the risk of sample loss and cross-contamination. By integrating weighing, balancing, and resuspension into a single liquid-liquid balancing device, the experimental process is simplified, reducing the need for multiple independent devices and thus lowering laboratory space requirements and equipment maintenance costs.
[0012] Furthermore, by reducing reliance on multiple devices, the costs of purchasing, maintaining, and operating multiple devices can be saved. Integrated operation reduces the time spent transferring samples between different devices and manual operations, significantly improving experimental efficiency. Automated weighing, balancing, and resuspending processes reduce human error, improving experimental consistency and repeatability.
[0013] Furthermore, the refrigeration module provides a stable low-temperature environment for the centrifuge bottles, which is particularly important for biochemical experiments that require specific temperatures, helping to improve the stability and reliability of experimental results. The use of a robotic arm allows for flexible transfer of centrifuge bottles between different devices, increasing the flexibility of sample handling. This automated workstation, through its integrated design and automated operation, not only improves experimental efficiency and safety but also ensures the accuracy and reliability of experimental results, while saving space and cost, and enhancing the level of automation in experiments.
[0014] In one alternative embodiment, the weighing sensor protrudes from the bottom surface of the weighing slot.
[0015] In one optional implementation, the weighing sensor corresponds one-to-one with a centrifuge bottle disposed in the weighing slot.
[0016] In an alternative embodiment, the weighing slots include first slots for placing large-capacity centrifuge bottles and second slots for placing small-capacity centrifuge bottles.
[0017] In an alternative embodiment, a plurality of first slots are provided, and each of the first slots is provided with a weighing sensor on the bottom surface.
[0018] In an alternative embodiment, the liquid distribution and balancing device further includes a tube rack slot and a tube rack provided on the tube rack slot, and the tube rack is provided with a plurality of insertion holes for placing centrifuge bottles at intervals.
[0019] In an alternative embodiment, the second slot is used to place the tube rack, and the bottom surface of the second slot is provided with a plurality of weighing sensors corresponding to the plurality of insertion holes.
[0020] In an alternative embodiment, the second slot is provided side by side with the tube rack slot.
[0021] In an alternative embodiment, the liquid distribution and balancing device further includes a cleaning tank, and the cleaning tank is provided side by side with the tube rack slot and is provided on the side of the tube rack slot away from the second slot.
[0022] In an alternative embodiment, the liquid distribution and balancing device further includes a workbench, and the weighing slots, the mechanical arm, the cleaning tank, and the tube rack slot are all provided on the workbench.
[0023] In an alternative embodiment, the workbench is provided with a refrigeration assembly.
[0024] In an alternative embodiment, the liquid suction mechanism is movably provided on the mechanical arm.
[0025] In an alternative embodiment, the liquid suction mechanism includes a liquid distribution needle or a pipette gun and a pump group connected to the liquid distribution needle or the pipette gun through a pipeline.
[0026] In an alternative embodiment, the liquid distribution needle or the pipette gun includes one or more, and a plurality of liquid distribution needles or pipette guns are provided side by side on the mechanical arm.
[0027] In an alternative embodiment, the refrigeration module is provided adjacent to the liquid distribution and balancing device.
[0028] In an alternative embodiment, the refrigeration slots include third slots for placing large-capacity centrifuge bottles and fourth slots for placing small-capacity centrifuge bottles.
[0029] In one optional embodiment, there are multiple third slots arranged in a row, and there are multiple fourth slots arranged in a row, with the third slots and the fourth slots arranged side by side.
[0030] In one optional embodiment, the refrigeration module further includes a bottle cap storage compartment for storing bottle caps, the bottle cap storage compartment being arranged side by side with the refrigeration slot.
[0031] In one alternative embodiment, the liquid pouring device includes a pouring arm, at least one collection tank located on one side of the pouring arm, and a drain tank for collecting waste liquid.
[0032] In one alternative embodiment, a drying module is provided on the inlet and outlet side of the cell disruption device.
[0033] In one alternative embodiment, the centrifugation apparatus includes a first rotor centrifuge for low-speed centrifugation and a second rotor centrifuge for high-speed centrifugation.
[0034] In one optional embodiment, the robotic arm is a central robotic arm, which is mounted on the base, and the refrigeration module, the liquid dispensing and balancing device, the centrifuge device, the cap opening device, the liquid pouring device, and the cell disruption device are arranged around the central robotic arm.
[0035] In one alternative embodiment, the robotic arm is a Cartesian coordinate system robotic arm, which is located outside the base.
[0036] In one alternative embodiment, the automated workstation further includes an operating room enclosed outside the base, the operating room being equipped with a communication interface.
[0037] In one optional implementation, the system further includes a main control module, which is electrically connected to the refrigeration module, the liquid dispensing and balancing device, the centrifugation device, the cap opening device, the liquid pouring device, and the cell disruption device, respectively. Attached Figure Description
[0038] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.
[0039] Figure 1 This is a schematic diagram of the overall structure of an automated workstation according to an embodiment of the present utility model;
[0040] Figure 2 is a perspective view as shown in the figure; Figure 1
[0041] Figure 3 is an exploded view as shown in the figure; Figure 1
[0042] Figure 4 is a structural schematic view of a liquid distribution and leveling device in the figure; Figure 1
[0043] Figure 5 is a structural schematic view of a pipe rack in the figure; Figure 1
[0044] Figure 6 is a structural schematic view of a liquid pouring device in the figure; Figure 1
[0045] Figure 7 is a structural schematic view of a cap opening device in the figure; Figure 1
[0046] Figure 8 is a structural schematic view of a wind-drying module in the figure; Figure 1
[0047] Figure 9 is a processing flow schematic view of an automatic work station according to an embodiment of the present application.
[0048] Explanation of reference signs:
[0049] 1, base; 2, refrigeration module; 21, third slot; 22, fourth slot; 23, bottle cap storage site; 3, liquid distribution and leveling device; 31, first slot; 32, second slot; 33, weighing sensor; 34, mechanical arm; 35, liquid suction mechanism; 36, pipe rack slot; 37, pipe rack; 371, jack; 38, cleaning tank; 39, workbench; 4, centrifugal device; 41, first rotor centrifuge; 42, second rotor centrifuge; 5, cap opening device; 51, tray; 511, bottle cap temporary storage site; 52, rotating clamping jaw; 53, clamping mechanism; 531, clamping plate; 5311, inner concave curved surface; 6, cell crushing device; 7, liquid pouring device; 71, pouring arm; 72, pouring arm groove; 73, collection slot; 74, liquid discharge slot; 8, wind-drying module; 81, hollow columnar cover; 82, fan; 9, mechanical hand; 10, small-capacity centrifuge bottle. DETAILED DESCRIPTION
[0050] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model below. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the protection scope of the utility model.
[0051] The embodiments of the utility model will be described below in combination with Figures 1 to 9
[0052] According to the embodiments of the utility model, as Figures 1 to 3 indicated, the utility model provides an automatic workstation mainly comprising a base 1 and a refrigeration module 2, a liquid distribution and leveling device 3, a centrifugal device 4, a cap opening device 5, a cell crushing device 6 and a liquid pouring device 7 arranged on the base 1.
[0053] Specifically, the refrigeration module 2 comprises refrigeration slots for storing centrifugal bottles.
[0054] Specifically, the liquid distribution and leveling device 3 comprises weighing slots corresponding to the specifications of the refrigeration slots and provided with weighing sensors 33 at the bottom, and a mechanical arm 34 arranged beside the weighing slots and provided with a liquid suction mechanism 35 for leveling and resuspending the target centrifugal bottle. It can be understood that the liquid suction mechanism 35 can suck and discharge liquid for leveling, or repeatedly suck liquid for resuspending. The refrigeration module 2 mainly provides a low-temperature environment for the centrifugal bottles placed thereon.
[0055] It can be understood that the weighing slots correspond to the specifications of the refrigeration slots, that is, for example, the refrigeration slots have 500mL and 50mL slots, and the weighing slots also have 500mL and 50mL slots. In this way, any specification of centrifugal bottle of the refrigeration slots has a corresponding placement position when moved to the weighing slots for leveling.
[0056] Specifically, the weighing slots and the refrigeration slots are both groove structures, which facilitate the stable placement of the centrifugal bottles in the groove structures.
[0057] The automatic workstation further comprises a mechanical hand 9 for transferring the centrifugal bottles between the refrigeration module 2, the liquid distribution and leveling device 3, the centrifugal device 4, the cap opening device 5, the cell crushing device 6 and the liquid pouring device 7 to perform leveling, centrifugation, cap opening and closing, cell crushing and liquid pouring operations.
[0058] The embodiment sets a weighing sensor 33 in the weighing groove, and the centrifugal bottle is placed in the weighing groove, which provides storage space for the centrifugal bottle and can automatically weigh. According to the weighing data, the liquid suction mechanism 35 of the liquid distribution and balancing device 3 is used to balance the centrifugal bottle, so as to ensure the uniformity of the weight of the centrifugal bottle placed in the centrifuge, thereby ensuring the balance in the centrifugal process, which is crucial for improving the centrifugal efficiency and separation effect.
[0059] Moreover, the liquid suction mechanism 35 can also add resuspension liquid to the concentrate obtained after one-step centrifugation of the centrifugal bottle and repeatedly suck to mix, so that the concentrate is resuspended in the resuspension liquid. As can be seen, the weighing, balancing and resuspension are all carried out on the liquid distribution and balancing device 3, which realizes the operation that needs three devices in the past, and now only one device can realize it, realizes the integration of multiple processes, helps to improve the efficiency of the steps before crushing and the automation level and experimental efficiency of the whole workstation, and the automatic operation reduces the number of sample transfers between different devices, reduces the risk of sample loss and cross contamination. By integrating weighing, balancing and resuspension in one liquid distribution and balancing device 3, the experimental process is simplified, the need for multiple independent devices is reduced, and the space occupation and equipment maintenance cost of the laboratory are reduced.
[0060] Further, since the dependence on multiple devices is reduced, the cost of purchasing, maintaining and operating multiple devices can be saved. Integrated operation reduces the time and manual operation of sample transfer between different devices, significantly improving the efficiency of the experiment. The automatic weighing, balancing and resuspension process reduces the error of human operation, improves the consistency and repeatability of the experiment.
[0061] In addition, the refrigeration module 2 provides a stable low-temperature environment for the centrifugal bottle, which is particularly important for biochemical experiments that need to be carried out at a specific temperature, and helps to improve the stability and reliability of the experimental results. The use of the mechanical hand 9 makes the centrifugal bottle flexible in transferring between different devices, improving the flexibility of sample processing. The automatic workstation improves the efficiency and safety of the experiment through integrated design and automatic operation, ensures the accuracy and reliability of the experimental results, saves space and cost, and improves the automation level of the experiment.
[0062] In a preferred embodiment, the base 1 is close to the experimenter on one side, and far from the experimenter on the other side. Since the refrigeration module 2 is used to place the pre-prepared test solution, in order to facilitate the experimenter to place, the refrigeration module 2 is preferably arranged on the base 1 close to the front side and on the right side. In order to facilitate the subsequent weighing and balancing steps, the liquid distribution and balancing device 3 is preferably arranged on the base 1 beside the refrigeration module 2, and the two are arranged adjacent to each other. The centrifuge device 4 and the cell crushing device 6 are preferably arranged on the base 1 close to the rear side. The drying module 8 is arranged at the inlet and outlet of the cell crushing device 6. The opening device 5 and the liquid pouring device 7 are preferably arranged on the left side of the base 1, and together with the refrigeration module 2 and the liquid distribution and balancing device 3, they enclose the four sides of the base 1. The robot 9 is preferably arranged in the middle of the base 1, which facilitates the movement of the robot 9 between the various modules. The layout can refer to Figure 2 .
[0063] In a specific embodiment, as Figure 4 shown, the weighing sensor 33 is protruded on the bottom surface of the weighing groove, which facilitates the centrifuge bottle to be inserted into the weighing groove and the bottle bottom to be in direct contact with the weighing sensor 33, thereby facilitating the weighing of the centrifuge bottle.
[0064] In a specific embodiment, the weighing sensor 33 corresponds to the centrifuge bottle arranged in the weighing groove, that is, if a weighing groove can only place one centrifuge bottle, the weighing groove is provided with only one weighing sensor 33, and if the weighing groove can place multiple centrifuge bottles, the weighing groove is provided with multiple weighing sensors 33, which ensures that each centrifuge bottle can be weighed.
[0065] In order to adapt to centrifuge bottles of different specifications, in a specific embodiment, the weighing groove includes a first groove 31 for placing large-capacity centrifuge bottles and a second groove 32 for placing small-capacity centrifuge bottles 10.
[0066] In a specific embodiment, multiple first grooves 31 are arranged at intervals. Since large-capacity centrifuge bottles occupy a large volume, each first groove 31 is suitable for placing one large-capacity centrifuge bottle (not shown in the figure), and therefore the bottom surface of each first groove 31 is provided with a corresponding weighing sensor 33 for weighing the corresponding large-capacity centrifuge bottle.
[0067] In a specific embodiment, the liquid distribution and balancing device 3 further includes a tube rack groove 36 and a tube rack 37 arranged on the tube rack groove 36, as Figure 5 shown, multiple insertion holes 371 for placing centrifuge bottles are arranged at intervals on the tube rack 37. By arranging the tube rack 37, multiple centrifuge bottles can be placed at one time, and the multiple centrifuge bottles can be transferred together with the tube rack 37.
[0068] In one specific embodiment, the tube rack 37 is generally square in shape, and the second slot 32 is also set to a matching square shape. The depth of the second slot 32 is less than the height of the tube rack 37. The tube rack 37 is placed in the second slot 32 and part of it is located outside the second slot 32, which facilitates the clamping of the tube rack 37. The bottom surface of the second slot 32 is provided with multiple weighing sensors 33 that correspond one-to-one with multiple insertion holes 371, so that the centrifuge bottle can fall directly onto the weighing sensor 33 after being inserted into the insertion hole 371.
[0069] The tube rack 37 includes at least two base plates and vertical plates at both ends of the base plates. Insertion holes 371 are provided on the base plates. The size of the insertion holes 371 is determined according to the centrifuge bottles to be placed. The number of base plates is determined according to the height of the centrifuge bottles. The structure of the tube rack 37 is prior art and will not be described in detail here.
[0070] In a specific embodiment, such as Figure 4 As shown, the second slot 32 and the pipe rack slot 36 are arranged side by side, which makes it easy for the pipe rack 37 to switch between the second slot 32 and the pipe rack slot 36 according to experimental needs. Moreover, the movement distance is short and the time spent is less.
[0071] In one specific embodiment, the liquid separation and balancing device 3 further includes a cleaning tank 38 for cleaning the liquid aspiration mechanism 35. The cleaning tank 38 is arranged side by side with the tube rack slot 36 and is located on the side of the tube rack slot 36 away from the second slot 32. That is, the cleaning tank 38, the tube rack slot 36, and the second slot 32 are arranged sequentially and complement each other, facilitating the movement of the tube rack 37 to the second slot 32 and also facilitating the movement of the liquid aspiration mechanism 35 located in the second slot 32 to the cleaning tank 38 for cleaning. The setting of the cleaning tank 38 not only improves cleaning efficiency and quality but also helps to save test solution, reduce operation time, and improve the repeatability and reliability of experiments.
[0072] In one specific embodiment, the liquid separation and leveling device 3 also includes a workbench 39, on which the weighing tank, robotic arm 34, washing tank 38, and tube rack slot 36 are all located, facilitating the integrated setup of the liquid separation and leveling device 3. The integration of all components onto a single workbench 39 makes operation more centralized and convenient, reducing the need to move samples and tools between different devices and improving work efficiency. By integrating multiple functions onto the workbench 39, the utilization of laboratory space is optimized, resulting in a more compact device layout and saving valuable laboratory space. The modular design of the device allows for flexible arrangement of the workbench structure as needed, and modules can be added or replaced according to experimental requirements.
[0073] In a specific embodiment, the workbench 39 is provided with a refrigeration assembly. The workbench can be assembled by multiple plates to form a cuboid structure with a cavity. For example, a semiconductor refrigeration sheet or other refrigeration assembly can be arranged in the cavity to cool the workbench. The refrigeration assembly can provide precise temperature control for the test solution or sample during the dispensing and matching process, which is crucial for biological experiments or chemical reactions that need to be performed at a specific temperature. For example, a semiconductor refrigeration module (thermoelectric module) can achieve high-precision temperature control (±0.05℃), which is very helpful to ensure the accuracy and repeatability of the experiment.
[0074] In a specific embodiment, the liquid suction mechanism 35 is movably arranged on the mechanical arm 34. In this way, the flexibility, efficiency and accuracy of the test solution matching can be improved, human errors can be reduced, and manpower can be saved. The combination of the liquid suction mechanism 35 and the mechanical arm 34 makes the process of sucking, transferring and releasing the test solution more accurate, replacing the traditional pipetting tool and automatically completing the tasks of high-precision matching and resuspension.
[0075] In a specific embodiment, the liquid suction mechanism 35 includes a dispensing needle or pipette and a pump set connected to the dispensing needle or pipette through a pipeline. The pump set provides power for the dispensing needle or pipette to add or suck liquid, and the liquid is transported through the pipeline.
[0076] In a specific embodiment, the dispensing needle or pipette includes one or more. If one is provided, only one operation can be performed at a time. If multiple dispensing needles or pipettes are used, multiple dispensing needles or pipettes can be arranged side by side on the mechanical arm 34. The side-by-side arrangement of the dispensing needles or pipettes can simultaneously process the liquid of multiple centrifuge bottles, significantly improving the efficiency of the experiment. The side-by-side dispensing needles or pipettes can be configured differently according to the experimental requirements, providing higher flexibility and versatility.
[0077] In a specific embodiment, the refrigeration module 2 is arranged adjacent to the dispensing and matching device 3, so that the centrifuge bottles on the refrigeration module 2 can be quickly transferred to the weighing slots of the dispensing and matching device 3, improving the transfer efficiency.
[0078] In a specific embodiment, the refrigeration slots include a third slot 21 for placing large-capacity centrifuge bottles and a fourth slot 22 for placing small-capacity centrifuge bottles 10 to accommodate centrifuge bottles of various specifications.
[0079] In a specific embodiment, the third slot 21 is provided with multiple third slots 21 arranged in a row, which can accommodate multiple large-capacity centrifuge bottles. The fourth slot 22 is provided with multiple fourth slots 22 arranged in a row, which can accommodate multiple small-capacity centrifuge bottles 10. The third slot 21 and the fourth slot 22 are arranged side by side to keep the arrangement of various centrifuge bottles in order.
[0080] Before placing the centrifuge bottle in the refrigeration tank, the bottle cap is removed. In one specific embodiment, the refrigeration module 2 also includes a bottle cap storage position 23 for storing the bottle cap. This arrangement provides a storage location for the removed bottle cap, making it convenient to retrieve the bottle cap from the storage position 23 for closing the cap later. Furthermore, the bottle cap storage position 23 is arranged side by side with the refrigeration tank, that is, the bottle cap storage position 23 is located on one side of the refrigeration tank, facilitating the removal and placement of the bottle cap.
[0081] In a specific embodiment, such as Figure 6 As shown, the liquid pouring device 7 includes a pouring arm 71, at least one collection slot 73 located on one side of the pouring arm 71, and a drain trough 74 for collecting waste liquid. The pouring arm 71 is provided with a pouring arm recess 72 for supporting centrifuge bottles of different capacities, such as 500mL centrifuge bottles and 50mL centrifuge bottles. The collection slot 73 can be provided in two ways: one for placing 500mL centrifuge bottles and the other for placing 50mL centrifuge bottles.
[0082] In one specific embodiment, a drying module 8 is provided on the inlet and outlet side of the cell disruption device 6. The cell disruption device 6 typically includes a soundproof outer shell, a cooling assembly, and a disruption assembly for disrupting cells located inside the outer shell. Cell disruption can be performed using one of the following methods: insertion of an ultrasonic probe into the sample for disruption; simultaneous immersion of the ultrasonic probe and centrifuge bottle in a coolant, with the ultrasonic energy transferred through the coolant to disrupt the cells; or injection of the sample into a high-pressure piston followed by high-speed extrusion through a small orifice for disruption. These three disruption methods are commonly used cell disruption methods and will not be detailed here. It should be noted that if the disruption components, such as the ultrasonic probe or high-pressure piston, are in direct contact with the sample, a corresponding cleaning assembly should also be provided to prevent cross-contamination of the sample.
[0083] Because the outer surface of the centrifuge bottles removed from the casing is covered with water droplets, these droplets can easily drip onto the base 1 or other equipment during transport, causing contamination. By installing a drying module 8 on one side of the inlet and outlet of the cell disruption device 6, the outer surface of the centrifuge bottles removed from the liquid can be quickly dried. Figure 8 As shown, the air-drying module 8 includes a fan 82 at the bottom for venting and a hollow cylindrical cover 81 on the platform for surrounding the centrifuge bottles. The airflow generated by the fan 82 removes the cooling water used to cool the centrifuge bottles during cell disruption.
[0084] In one specific embodiment, the centrifuge device 4 includes a first rotor centrifuge 41 for low-speed centrifugation and a second rotor centrifuge 42 for high-speed centrifugation. The first rotor centrifuge 41 can be a horizontal rotor centrifuge, centrifuging at an acceleration of less than 4000g (usually 3000g in practice), and the second rotor centrifuge 42 can be a high-speed angular rotor centrifuge, centrifuging at an acceleration equivalent to more than 10000g (usually 12000g).
[0085] Further, the horizontal rotor centrifuge comprises an automatically openable cabin door, an automatically positionable rotor and a refrigeration module, and proper low temperature (e.g. 4℃) during centrifugation helps to protect proteins from degradation. When it is necessary to take or place the centrifuge bottles, the cabin door is opened, and the rotor is rotated to the position below the cabin door with the assistance of the positioning mechanism, and the robot 9 completes the taking or placing of the centrifuge bottles through the cabin door.
[0086] Correspondingly, the high-speed angle rotor centrifuge comprises an automatically openable cabin door, an automatically positionable rotor and a refrigeration module, and proper low temperature (e.g. 4℃) during centrifugation helps to protect proteins from degradation. When it is necessary to take or place the centrifuge bottles, the cabin door is opened, and the rotor is rotated to the designated angle with the assistance of the positioning mechanism, and the robot 9 subsequently completes the taking or placing of the centrifuge bottles.
[0087] In a specific embodiment, the robot 9 is a central robot, and the refrigeration module 2, the liquid distribution and leveling device 3, the centrifuge device 4, the cap opening device 5, the liquid pouring device 7 and the cell disruption device 6 are arranged around the central robot. Thus, the central robot is not far from each device, and it is convenient and time-saving for the central robot to move the centrifuge bottles between the devices.
[0088] In another embodiment, the robot 9 can also be a Cartesian coordinate system robot, which can move along six degrees of freedom of X, Y and Z axes. By setting a target point in the Cartesian coordinate system, the robot 9 can accurately move to the predetermined position to achieve precise control. In order to reduce the space occupation of the base 1, the Cartesian coordinate system robot is preferably arranged outside the base 1.
[0089] In a specific embodiment, the automation workstation further comprises an operation room arranged outside the base 1, so as to provide a relatively clean and closed environment for each device and the robot 9, which can effectively prevent pollutants in the external environment from entering the interior of the device, and protect the precision instruments and mechanical parts inside the automation workstation from the influence of environmental factors such as dust and humidity, thereby improving the reliability and durability of the device. In order to automatically control each device, the operation room is provided with a communication interface, so that the automation workstation can exchange data and communicate with external systems.
[0090] In a specific embodiment, a host module (not shown in the figure) is also included, which can be a host computer, and is electrically connected with the refrigeration module 2, the liquid distribution and leveling device 3, the centrifugal device 4, the cover opening device 5, the liquid pouring device 7 and the cell crushing device 6 for communication between each other. So that the host module can accurately control the working state of the refrigeration module 2, realize temperature regulation and maintain the required low-temperature environment, which is crucial for biological experiments or chemical reactions that need to be carried out at a specific temperature. The host module can control the operation of the liquid distribution and leveling device 3, including the distribution and leveling of the liquid, to ensure the accuracy and repeatability of the liquid treatment during the experiment. The host module allows the speed and running time of the centrifugal device 4 to be controlled, which is necessary for separating samples or cell components of different densities. The host module controls the cover opening device 5 through electrical connection, which can automatically open and close the container cover, reducing manual operation and improving the safety and efficiency of operation. The host module can control the liquid pouring device 7 to realize the automation of liquid pouring, reduce manual operation, reduce labor intensity, and avoid liquid cross-flow splashing. The host module controls the cell crushing device 6 through electrical connection, which can accurately crush cells to release substances inside the cells, which is very important for subsequent experimental analysis or biological engineering applications. The electrical connection of the host module with these devices makes the operation of the entire automated workstation more integrated and automated, improving the accuracy, repeatability and efficiency of the experiment, while also enhancing the safety of the operation.
[0091] According to the embodiments of the present application, as shown in Figure 9 The processing flow of the automated workstation is as follows:
[0092] S10, the prepared test solution is divided into multiple centrifugal bottles and stored in the refrigeration slots of the refrigeration module 2;
[0093] S20, the centrifugal bottles are gripped by the mechanical hand 9 to the weighing slots of the liquid distribution and leveling device 3 for weighing and leveling;
[0094] S30, the centrifugal bottles after leveling are capped and transferred to the centrifugal device 4 for centrifugation, to obtain the concentrate and supernatant settled at the bottom of the centrifugal bottles;
[0095] S40, the centrifugal bottles after centrifugation are uncapped and transferred to the liquid pouring device 7, and the liquid pouring device 7 is used to pour off the supernatant of the centrifugal bottles;
[0096] S50, the centrifugal bottles are transferred again to the weighing slots of the liquid distribution and leveling device 3, and the suction liquid mechanism 35 is used to add resuspension liquid to the centrifugal bottles and repeatedly suck to mix the concentrate and the resuspension liquid;
[0097] S60, the mixed resuspension liquid is leveled and transferred to the cell crushing device 6 for crushing;
[0098] S70, cap the centrifuge bottle after crushing and transfer it to the centrifuge device 4 for centrifugation to obtain supernatant containing target substances.
[0099] The steps of the above process can be further refined as follows:
[0100] Dispensing of test solution
[0101] Preparation of centrifuge bottles: Ensure that the centrifuge bottles are clean, uncontaminated, and suitable for the refrigeration slots of the refrigeration module 2.
[0102] Dispensing of test solution: Use a dispenser or a pipette to dispense the prepared test solution into multiple centrifuge bottles.
[0103] Storage of refrigeration slots: Place the dispensed centrifuge bottles into the refrigeration slots of the refrigeration module 2, and set an appropriate temperature to maintain the stability of the test solution.
[0104] Weighing and balancing
[0105] Mechanical hand 9 clamping: Start the mechanical hand 9 and use the clamp to clamp the centrifuge bottle.
[0106] Transfer to weighing slots: Transfer the centrifuge bottle to the weighing slots of the dispensing and balancing device 3.
[0107] Weighing and balancing: Use the weighing function of the dispensing and balancing device 3 to weigh the centrifuge bottle, and add or reduce the test solution as needed to achieve balancing.
[0108] Centrifugal separation
[0109] Cap: Cap the balanced centrifuge bottle.
[0110] Transfer to centrifuge device 4: Use the mechanical hand 9 to transfer the centrifuge bottle to the centrifuge device 4.
[0111] Centrifugal operation: Set the centrifugal speed and time, start the centrifuge device 4, and make the precipitate in the test solution settle at the bottom of the centrifuge bottle, while separating the supernatant.
[0112] Pouring of supernatant
[0113] Uncover: Uncover the centrifuged centrifuge bottle.
[0114] Transfer to pouring device: Transfer the centrifuge bottle to the liquid pouring device 7.
[0115] Pouring of supernatant: Use the liquid pouring device 7 to pour out the supernatant in the centrifuge bottle, and retain the precipitate.
[0116] Addition of resuspension solution
[0117] Again transfer to weighing slots: Transfer the processed centrifuge bottle to the weighing slots of the dispensing and balancing device 3 again.
[0118] Add resuspension liquid: use the pipetting mechanism 35 to add an appropriate amount of resuspension liquid to the centrifuge bottle.
[0119] Mix: mix the sediment and resuspension liquid evenly by repeated pipetting.
[0120] Resuspension liquid balancing
[0121] Balance the resuspension liquid: weigh and balance the mixed resuspension liquid to ensure that the amount of resuspension liquid in each centrifuge bottle is consistent.
[0122] Cell disruption
[0123] Transfer to cell disruption device 6: transfer the balanced resuspension liquid centrifuge bottle to the cell disruption device 6.
[0124] Cell disruption: start the cell disruption device 6 to disrupt the cells in the resuspension liquid and release the target substance.
[0125] Collect the target substance
[0126] Close the lid: close the lid of the centrifuge bottle after the disruption process.
[0127] Second centrifugation: place the centrifuge bottle back into the centrifuge device 4 for a second centrifugation to separate the supernatant containing the target substance.
[0128] Collect the supernatant: after centrifugation, collect the supernatant containing the target substance, ready for subsequent analysis or application.
[0129] As can be seen, the automated workflow reduces manual operation, saves time, and improves the efficiency of processing test solutions. Precise weighing and balancing are performed using the mechanical hand 9 and the weighing sensor 33, ensuring the balance during centrifugation, improving centrifugation efficiency and separation effect. Automated operation reduces the number of times the sample is transferred between different devices, reducing the risk of sample loss. Weighing, balancing, and resuspension are all performed on the dispensing and balancing device 3, which can now be achieved with only one device, helping to improve the automation level of the entire workstation. Automated operation reduces the number of times the sample is transferred between different devices, reducing the risk of sample loss and cross-contamination. By integrating weighing, balancing, and resuspension into one dispensing and balancing device 3, the experimental process is simplified, the need for multiple independent devices is reduced, and the space occupied by the laboratory and the cost of equipment maintenance are reduced.
[0130] Specifically, as Figure 7As shown, the cap opening device 5 comprises a rotating gripper 52 for opening the cap, a mechanism for moving the rotating gripper 52 between a centrifuge bottle position and a cap temporary storage position, a tray 51 for temporarily storing the opened cap, and a clamping mechanism 53 for clamping the centrifuge bottle to prevent it from rotating with the cap during the opening and closing process.
[0131] Specifically, the clamping mechanism 53 can comprise a pair of clamping plates 531 and a pair of elastic clamping blocks 532 mounted on the opposite sides of the pair of clamping plates 531, and each of the pair of elastic clamping blocks 532 is provided with a corresponding concave arc surface 5321 on the opposite side. Each concave arc surface 5321 is smaller than a semicircle. In this way, the centrifuge bottle can be easily clamped and the centrifuge bottle is prevented from rotating during the opening process. Moreover, the elastic clamping blocks 532 have a certain elasticity, which can protect the centrifuge bottle from being damaged by clamping, and can fit the surface of different centrifuge bottles through certain deformation, making it easier to clamp the centrifuge bottle.
[0132] Further, the upper surface of the tray 51 is provided with a plurality of cap temporary storage positions 511, and the pair of clamping plates 531 is located on the upper surface of the tray 51 and is spaced apart from the cap temporary storage positions 511. The tray 51 is provided with a through slot at a position between the pair of clamping plates 531. A clamping plate driving source is installed below the tray 51, and the top of the clamping plate driving source passes through the through slot. The top of the clamping plate driving source is provided with two driving sliding blocks. The two driving sliding blocks can move towards or away from each other under the driving of the clamping plate driving source. The middle region of each clamping plate 531 is provided with an upper and lower through hole, and the driving sliding blocks are provided with a protruding rod matched with the through hole. The protruding rod is inserted into the through hole and fixed on the driving sliding block by screws, so that the pair of clamping plates 531 can be driven to move towards or away from each other by the clamping plate driving source, thereby changing the distance between the pair of clamping plates 531, facilitating clamping or releasing the centrifuge bottle, and also adapting to different specifications of the centrifuge bottle.
[0133] In specific work, the pair of clamping plates 531 is driven to open, the centrifuge bottle is placed between the pair of clamping plates 531, and then the pair of clamping plates 531 is driven to clamp the centrifuge bottle. The centrifuge bottle is fixed by the opposite concave arc surfaces 5321 of the elastic clamping blocks 532 abutting against the surface of the centrifuge bottle. The rotating gripper 52 is driven to move directly above the centrifuge bottle, and the rotating gripper 52 is driven to clamp and rotate the cap to complete the opening of the cap on the centrifuge bottle. The closing of the cap is the reverse process of the opening, which will not be described here. In the working process, the rotating gripper 52 is adapted to the opening and closing of the caps of multiple or various centrifuge bottles, and the corresponding caps are placed on the cap temporary storage positions 511 of the tray 51, thereby realizing automatic opening and closing.
[0134] In a specific embodiment, the above steps further comprise:
[0135] S11, the prepared test solution is divided into multiple large-capacity centrifuge bottles and stored in the refrigeration slots of the refrigeration module 2;
[0136] S21, the large-capacity centrifuge bottles are gripped by the mechanical arm 9 to the weighing slots of the liquid distribution and leveling device 3 for weighing and leveling;
[0137] S31, the large-capacity centrifuge bottles after leveling are capped and transferred to the centrifuge device 4 for low-speed centrifugation, to obtain the concentrate and supernatant settled at the bottom of the centrifuge bottle;
[0138] S41, the large-capacity centrifuge bottles after centrifugation are uncapped and transferred to the liquid pouring device 7, and the supernatant of the large-capacity centrifuge bottles is poured off by the liquid pouring device 7;
[0139] S51, the large-capacity centrifuge bottles are transferred to the weighing slots of the liquid distribution and leveling device 3 again, the resuspension solution is added to the large-capacity centrifuge bottles by the suction liquid mechanism 35, and the concentrate is mixed with the resuspension solution by repeatedly sucking by the suction liquid mechanism 35;
[0140] S61, the mixed resuspension solution is divided into multiple small-capacity centrifuge bottles 10 and leveled, and the small-capacity centrifuge bottles 10 are transferred to the cell crushing device 6 for crushing;
[0141] S71, the small-capacity centrifuge bottles 10 after crushing are capped and transferred to another centrifuge device 4 for high-speed centrifugation, to obtain the supernatant containing the target substance.
[0142] The existing scheme is to divide the test solution into several small-capacity centrifuge bottles 10 for leveling and subsequent centrifugation, and the subsequent resuspension is also carried out in the small-capacity centrifuge bottles 10. This process includes multiple steps of division, leveling, centrifugation and cleaning, which takes a long time and leads to low efficiency. The embodiment directly divides the test solution into large-capacity centrifuge bottles for leveling and subsequent centrifugation, and then divides the concentrate obtained by centrifugation into multiple small-capacity centrifuge bottles 10 after resuspension, which reduces the division step and saves a lot of time. It highlights that large-volume liquid is used in large-capacity containers, and small-volume liquid is used in small-capacity containers, rather than dividing large-volume liquid into multiple small-capacity containers for processing, which greatly improves the efficiency.
[0143] It should be noted that the capacity of the large-capacity centrifuge bottles in the embodiment can be 1L, 500mL, etc., and the capacity of the small-capacity centrifuge bottles 10 can be 50mL, 15mL, etc.
[0144] It should be noted that the uncapping and capping actions can be performed on the uncapping device 5 or by the rotating clamping function of the mechanical arm 9.
[0145] In one specific embodiment, in the step of dispensing the mixed resuspended liquid into multiple small-capacity centrifuge bottles 10 and balancing them, the aspiration mechanism 35 is cleaned inside and out after each dispensing into a small-capacity centrifuge bottle 10 to avoid cross-contamination.
[0146] In one specific embodiment, in the steps of dispensing the mixed resuspension into multiple small-capacity centrifuge bottles 10 and balancing them, and transferring the small-capacity centrifuge bottles 10 to the cell disruption device 6 for disruption, the multiple small-capacity centrifuge bottles 10 are placed on a tube rack 37 and balanced. The tube rack 37 containing the multiple small-capacity centrifuge bottles 10 is then clamped and placed in the cell disruption device 6 for simultaneous disruption, which helps to improve efficiency.
[0147] To describe the operation process of this embodiment in detail, the biological reaction system for expressing proteins in vivo using E. coli is used as an example. In this embodiment, the capacity of the large-capacity centrifuge bottle is 500 mL, and the capacity of the small-capacity centrifuge bottle 10 is 50 mL.
[0148] Once the equipment is started, each piece of equipment completes operations such as position initialization, pre-cooling, and pipeline pre-cleaning.
[0149] The experimenters dispensed the E. coli culture medium prepared by the external bioreactor into four 500mL conical-bottom centrifuge bottles and transferred them to the refrigeration tank of refrigeration module 2 for temporary storage, with the bottle caps placed in bottle cap storage position 23.
[0150] The robotic arm 9 picks up the centrifuge bottle and places it into the weighing tank of the liquid separation and balancing device 3. A weighing sensor 33 at the bottom of the tank can collect the weight of the centrifuge bottle. When an uneven weight is detected, the dispensing needle on the robotic arm 34 will pump buffer solution to balance the centrifuge bottle.
[0151] The robotic arm 9 has continuous rotation and clamping functions. After balancing, it individually caps the centrifuge bottles and then clamps them into the horizontal rotor centrifuge. The horizontal rotor centrifuge has four centrifuge baskets. After each centrifuge bottle is placed, the basket rotates at a certain angle to rotate the next basket to below the door, making it convenient for the robotic arm 9 to place the next centrifuge bottle.
[0152] The horizontal rotor centrifuge is started and four 500mL centrifuge bottles are centrifuged. At this time, the E. coli settles to the bottom of the centrifuge bottle under the action of centrifugal force.
[0153] After centrifugation, the robotic arm 9 removes the 500mL centrifuge bottle and places it into the rotatable tilting arm groove 72 of the liquid tilting device 7, clamping and opening the cap. Then, the tilting arm 71 of the liquid tilting device 7 rotates the centrifuge bottle counterclockwise approximately half a turn, pouring the waste culture medium into the drain trough 74 below, and collecting it through the tubing into an external waste container. After tilting, the tilting arm 71 returns to its initial position, releasing the clamp on the centrifuge bottle.
[0154] The robot 9 picks up the tube rack 37 with the 50 mL centrifuge bottles in the tube rack slot 36 into the clamping mechanism 53 of the cap opening device 5, the clamping mechanism 53 clamps the centrifuge bottles through the open area of the side of the tube rack 37, then the moving mechanism moves above the centrifuge bottles, the rotating clamping jaw 52 rotates the cap of the centrifuge bottles, then the moving mechanism moves above the tray 51 to temporarily store the cap. Finally, the robot 9 clamps the tube rack 37 with the opened centrifuge bottles back to the weighing slot.
[0155] The robot 9 picks up the four 500 mL centrifuge bottles after pouring into the weighing slot of the dispensing and leveling device 3, the robot arm 34 drives the dispensing needle to add the resuspension liquid to the four centrifuge bottles respectively, and repeatedly aspirates until the bacteria at the bottom are resuspended and distributed into the resuspension liquid. Then the dispensing needle collects the resuspension liquid and transfers it into the 50 mL centrifuge bottle in the tube rack 37 in the weighing slot. The dispensing needle needs to flow clean the inner and outer pipelines in the cleaning tank 38 after each resuspension and transfer.
[0156] Then it is ready to be transferred to the cell disruption device 6 for disruption operation. According to the different disruption methods of the cell disruption device 6, it is described separately.
[0157] When non-contact ultrasonic disruption is used:
[0158] After the resuspension liquid is transferred from the 500 mL centrifuge bottle to the 50 mL centrifuge bottle, the dispensing needle levels the four 50 mL centrifuge bottles according to the weighing results (for example, taking one tube as a reference, aspirating and discarding the heavier ones, and supplementing the liquid to level the lighter ones).
[0159] The robot 9 clamps the leveled centrifuge bottles together with the tube rack 37 into the cap opening device 5, and closes the cap of the bottle opened in the previous step. Then the cell disruption device 6 is opened, and the robot 9 clamps the capped centrifuge bottles into the cell disruption device 6 respectively for cell disruption.
[0160] After cell disruption, the robot 9 evenly distributes the centrifuge bottles into the rotors of the opened high-speed angle rotor centrifuge. The high-speed angle rotor centrifuge is closed and high-speed centrifuged to separate the bacterial residue and the supernatant containing the target protein.
[0161] When contact ultrasonic disruption or high-pressure disruption is used:
[0162] After the resuspension liquid is transferred from the 500 mL centrifuge bottle to the 50 mL centrifuge bottle, the robot 9 directly clamps the centrifuge bottles into the opened cell disruption device 6 for cell disruption.
[0163] After cell disruption, the robot 9 clamps the centrifuge bottles back to the weighing slot of the dispensing and leveling device 3, and uses the dispensing needle to level the four 50 mL centrifuge bottles with buffer according to the weighing results.
[0164] Mechanical hand 9 clamps the balanced centrifuge bottle together with the tube rack 37 into the cap opening device 5, and closes the cap of the bottle opened in the previous step. Mechanical hand 9 inserts the centrifuge bottles into the rotor of the high-speed angle rotor centrifuge respectively. After the high-speed angle rotor centrifuge is capped, high-speed centrifugation is performed to separate the bacterial residue and the supernatant containing the target protein.
[0165] During the centrifugation process, the mechanical hand 9 transfers the brand new centrifuge bottle together with the tube rack 37 to the cap opening device 5 to open the cap, and then places it into the collection slot 73.
[0166] After the centrifugation is completed, the mechanical hand 9 transfers the centrifuge bottle from the high-speed angle rotor centrifuge to the tube rack 37 of the cap opening device 5, and the cap opening device 5 opens the cap of the centrifuge bottle. Then the mechanical hand 9 transfers the tube rack 37 with the centrifuge bottle after opening the cap to the pouring arm groove 72 of the liquid pouring device 7.
[0167] After the pouring is completed, the solution containing the target protein is obtained. According to the experimental requirements, the user can choose to store the centrifuge bottle in the metal bath slot after capping and refrigerate, or transfer it to other devices for protein purification.
[0168] The device automatically cleans the pipeline according to the needs, and discards the waste consumables according to the process.
[0169] Although the embodiments of the present application are described in conjunction with the drawings, various modifications and changes can be made by those skilled in the art without departing from the spirit and scope of the present application, and such modifications and changes fall within the scope defined by the appended claims.
Claims
1. An automated workstation characterized by, The application relates to a centrifugal sample processing system. The centrifugal sample processing system comprises: a base (1); a refrigeration module (2) arranged on the base (1) and comprising refrigeration slots for storing centrifugal bottles; a liquid distribution and re-suspension device (3) arranged on the base (1) and comprising weighing slots corresponding to the refrigeration slots and provided with weighing sensors (33) at the bottom, and a mechanical arm (34) arranged beside the weighing slots and provided with a liquid suction mechanism (35) for re-suspending and re-distributing target centrifugal bottles; a centrifugal device (4), a cap opening device (5), a cell crushing device (6) and a liquid pouring device (7) arranged on the base (1) respectively; 2. The automated workstation of claim 1, wherein, a mechanical hand (9) for transferring centrifugal bottles between the refrigeration module (2), the liquid distribution and re-suspension device (3), the centrifugal device (4), the cap opening device (5), the cell crushing device (6) and the liquid pouring device (7).
3. The automated workstation of claim 2, wherein, The weighing sensors (33) are arranged on the bottom surface of the weighing slots.
4. The automated workstation of claim 3, wherein, The weighing sensors (33) correspond to the centrifugal bottles arranged in the weighing slots.
5. The automated workstation of claim 4, wherein, The weighing slots comprise first slots (31) for placing large-capacity centrifugal bottles and second slots (32) for placing small-capacity centrifugal bottles (10).
6. The automated workstation of claim 4, wherein, The first slots (31) are arranged at intervals, and each first slot (31) is provided with a corresponding weighing sensor (33) on the bottom surface.
7. The automated workstation of claim 6, wherein, The liquid distribution and re-suspension device (3) further comprises a tube rack slot (36) and a tube rack (37) arranged on the tube rack slot (36), and the tube rack (37) is provided with a plurality of jack holes (371) for placing centrifugal bottles at intervals.
8. The automated workstation of claim 6, wherein, The second slot (32) is used for placing the tube rack (37), and the bottom surface of the second slot (32) is provided with a plurality of weighing sensors (33) corresponding to the plurality of jack holes (371).
9. The automated workstation of claim 6, wherein, The second slot (32) is arranged side by side with the tube rack slot (36).
10. The automated workstation of claim 9, wherein, The liquid distribution and re-suspension device (3) further comprises a cleaning tank (38), which is arranged side by side with the tube rack slot (36) and is arranged on the side of the tube rack slot (36) away from the second slot (32).
11. The automated workstation of claim 10, wherein, The liquid distribution and re-suspension device (3) further comprises a workbench (39), and the weighing slots, the mechanical arm (34), the cleaning tank (38) and the tube rack slot (36) are all arranged on the workbench (39).
12. The automated workstation of claim 1, wherein, The workbench (39) is provided with a refrigeration assembly.
13. The automated workstation of claim 1, wherein, The liquid suction mechanism (35) is movably arranged on the mechanical arm (34).
14. The automated workstation of claim 13, wherein, The liquid suction mechanism (35) comprises a liquid distribution needle or a pipette and a pump group connected with the liquid distribution needle or the pipette through a pipeline.
15. The automated workstation of claim 1, wherein, The liquid distribution needle or the pipette comprises one or more, and a plurality of liquid distribution needles or pipettes are arranged side by side on the mechanical arm (34).
16. The automated workstation of claim 1, wherein, The refrigeration module (2) is arranged adjacent to the liquid distribution and re-suspension device (3). The refrigeration slots comprise third slots (21) for placing large-capacity centrifugal bottles and fourth slots (22) for placing small-capacity centrifugal bottles (10).
17. The automated workstation of claim 16, wherein, The third slots (21) are provided in plurality, the plurality of third slots (21) are arranged in rows, the fourth slots (22) are provided in plurality, the plurality of fourth slots (22) are arranged in rows, and the third slots (21) and the fourth slots (22) are arranged side by side.
18. The automated workstation of claim 1, wherein, The refrigeration module (2) further comprises a bottle cap storage site (23) for storing bottle caps, and the bottle cap storage site (23) is arranged side by side with the refrigeration slots.
19. The automated workstation of claim 1, wherein, The liquid pouring device (7) comprises a pouring arm (71) and at least one collection slot (73) and a liquid discharge groove (74) for collecting waste liquid arranged on one side of the pouring arm (71).
20. The automated workstation of claim 1, wherein, The import and export side of the cell crushing device (6) is provided with a air-drying module (8).
21. The automated workstation of claim 1, wherein, The centrifugal device (4) comprises a first rotor centrifuge (41) for low-speed centrifugation and a second rotor centrifuge (42) for high-speed centrifugation.
22. The automated workstation of any one of claims 1 to 21, wherein, The mechanical hand (9) is a central mechanical hand (9), which is arranged on the base (1), and the refrigeration module (2), the liquid distribution and leveling device (3), the centrifugal device (4), the cap opening device (5), the liquid pouring device (7) and the cell crushing device (6) are arranged around the central mechanical hand (9).
23. The automated workstation of any one of claims 1 to 21, wherein, The mechanical hand (9) is a Cartesian coordinate system mechanical hand (9), which is arranged outside the base (1).
24. The automated workstation of any one of claims 1 to 21, wherein, The automatic work station further comprises an operation room arranged outside the base (1), and the operation room is provided with a communication interface.
25. The automated workstation of any one of claims 1 to 21, wherein, Further comprising a main control module, and the main control module is electrically connected with the refrigeration module (2), the liquid distribution and leveling device (3), the centrifugal device (4), the cap opening device (5), the liquid pouring device (7) and the cell crushing device (6) respectively.