Smear preparation equipment suitable for membrane bacteria-collecting smear

Abstract

The utility model discloses smear preparation equipment suitable for a membrane bacteria-collecting smear, which belongs to the field of medical detection, and comprises a holding part and a sampling part, a sampling tube is arranged in the holding part, the sampling tube is communicated with the sampling part in a sealing way, and the other end of the sampling tube is connected with air pumping and injecting equipment. The utility model aims to provide the smear preparation equipment suitable for the membrane bacteria-collecting smear, and the detection cost of the microfiltration membrane can be reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical detection field, concretely is a kind of suitable for film bacterial collection smear preparation equipment. BACKGROUND

[0002] When cell, bacteria detection is carried out using filter membrane (microporous filter membrane) as described in the patent with announcement number CN102580548B, it needs to use the technical means as described in the patent with announcement number CN106248978B to intercept bacteria, cell. Subsequently, the microporous filter membrane is fixed on the glass slide using the technical solution as proposed in the patent with announcement number CN108760458B, so as to carry out subsequent operations such as microscopic examination. As can be seen, under the existing technical framework, cell, bacteria detection using microporous filter membrane not only has high equipment cost, but also puts forward corresponding strict requirements for detection place, resulting in high detection cost of microporous filter membrane, which limits the wide promotion and application of microporous filter membrane technology to some extent. SUMMARY

[0003] The utility model aims at above -mentioned problem, provides a kind of suitable for film bacterial collection smear preparation equipment, and microporous filter membrane's detection cost can be reduced.

[0004] To achieve the above object, the utility model adopts the technical scheme of a kind of suitable for film bacterial collection smear preparation equipment, it includes holding part, sampling part, the sampling tube is arranged in the holding part, the sampling tube is sealed with sampling part intercommunication, the sampling tube other end is connected with air extraction equipment. The negative pressure generated by air extraction equipment is extracted specimen. After specimen is absorbed, microporous filter membrane is fixed on the slide using sampling part, to reduce the detection cost of microporous filter membrane.

[0005] Further, to connect sampling tube and sampling part, one end of the sampling tube is provided with a suction and exhaust pipe, and a flow channel adapted to the suction and exhaust pipe is provided on the sampling part along its axial direction, and the flow channel is open at both ends.

[0006] Further, to increase the sampling area, a counterbore is provided at the lower end of the sampling part, and the lower end of the flow channel is open at the bottom of the counterbore, and the counterbore is used to expand the sampling area.

[0007] Further, when the suction head is used with the microporous filter membrane cup as described in the patent with announcement number CN106248978B, a support part is provided in the counterbore to avoid breakage of the microporous filter membrane, and through holes are provided on the support part at intervals, and when specimen is extracted, the support part presses against the microporous filter membrane to avoid excessive deformation and breakage of the microporous filter membrane.

[0008] Further, the sampling part and the holding part are connected by a soft glue part, and a through hole communicated with the flow channel is arranged on the soft glue part, so that the holding part and the sampling part are quickly and tightly assembled together.

[0009] Further, in order to fix the microporous filter membrane on the slide, a heating part is arranged at the lower end of the sampling part, so that the microporous filter membrane is melted and adhered to the slide.

[0010] Further, the control button for controlling the working of the heating part is arranged on the holding part, so as to facilitate the operation of the detection personnel.

[0011] Further, the slide is provided with a glue layer, so as to fix the microporous filter membrane on the slide by gluing.

[0012] The beneficial effects of the present application are as follows: when the specimen is sucked, the sampling part and the microporous filter membrane carrier are assembled together, then the microporous filter membrane is immersed in the specimen, when the air suction and injection equipment works, the inactivated specimen (liquid or gas) enters the sampling tube through the microporous filter membrane, and the cells and bacteria in the specimen are intercepted by the microporous filter membrane; then, the microporous filter membrane is pressed and fixed on the slide, so as to quickly complete the preparation, thereby reducing the detection cost of the microporous filter membrane. In this way, the sampling and detection can be separated. When needed, a large number of collection points can be quickly set, and the bacteria collection and preparation can be completed by simple operation. Then, the specimen is uniformly collected and detected, so that a long-term investigation mechanism of the infection source can be established, which not only improves the detection efficiency, but also reduces the detection cost. At the same time, the centralized staining and reading of the slide facilitate the statistical archiving work and ensure the detection quality. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 It is a side view structural schematic diagram of the present application in the preparation state.

[0014] Figure 2 It is a schematic diagram of the assembly structure of the present application and the microporous filter membrane carrier.

[0015] Figure 3 It is a side view structural schematic diagram of the preparation equipment in embodiment 1.

[0016] Figure 4 It is a side view structural schematic diagram of the preparation equipment in embodiment 2.

[0017] Figure 5 It is a schematic diagram of the membrane carrier structure.

[0018] The text labels in the figure represent: 1. Grip part; 2. Sampling part; 3. Sampling tube; 4. Drain tube; 5. Flow channel; 6. Countersink; 7. Support part; 8. Soft rubber part; 9. Heating part; 10. Control button; 11. Carrier; 12. Cup body; 13. Microporous filter membrane. Detailed Implementation

[0019] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be described in detail below with reference to the accompanying drawings. The description in this part is only exemplary and explanatory, and should not be used to limit the scope of protection of this utility model in any way.

[0020] Example 1, such as Figures 1-3 , Figure 5 As shown, the structure of this embodiment is as follows: a slide preparation device suitable for membrane-collected bacterial smears, which includes a gripping part 1 and a sampling part 2. A sampling tube 3 is provided inside the gripping part 1. The sampling tube 3 is sealed and connected to the sampling part 2. To connect the sampling tube 3 and the sampling part 2, matching internal and external threads can be provided on the side walls of the gripping part 1 and the sampling part 2. By rotating the gripping part 1 or the sampling part 2, the gripping part 1 and the sampling part 2 are assembled together. At this time, the air hole at the lower end of the sampling tube is located in the sealed space formed by the gripping part 1 and the sampling part 2, thereby achieving a sealed connection; or the gripping part 1 and the sampling part 2 are nested and connected, with the gripping part 1 inserted into the sampling part 2, or the sampling part 2 inserted into the gripping part 1, so as to enclose the air hole at the lower end of the sampling tube to achieve a sealed connection. In this embodiment, the sampling tube 3 is provided with a drain tube 4 at its lower end, and the drain tube 4 is open at both ends. The sampling part 2 is provided with a flow channel 5 along its axial direction that is adapted to the drain tube 4. The flow channel 5 is open at both ends. At the same time, the sampling part 2 is provided with a soft rubber part 8 at one end of the holding part 1. The soft rubber part 8 can be made of soft rubber materials such as silicone, rubber, and thermoplastic polyurethane elastomer. The soft rubber part 8 is provided with a through hole that communicates with the flow channel 5. The outer wall of the soft rubber part 8 is interference-fitted with the inner wall of the holding part 1, and the through hole is interference-fitted with the outer wall of the drain tube 4. By inserting the soft rubber part 8 into the holding part 1, the drain tube 4 extends into the through hole, thereby achieving a sealed connection between the sampling tube 3 and the sampling part 2.

[0021] The other end of the sampling tube 3 is connected to an air injection device (not shown in the figure) via a connecting pipe. The air injection device can be an existing air pump, fan, or other existing equipment capable of both extracting and blowing air. Alternatively, the air injection device may include an extraction pipe sealed to the connecting pipe, with a matching piston installed inside. The piston moves along the extraction axis under the action of existing linear drive devices such as electric push rods or linear modules. When the piston moves away from one end of the connecting pipe, air is extracted; when the piston moves closer to the connecting pipe, air is blown into the sampling tube 3.

[0022] The lower end of the sampling part 2 is provided with a circle of heating part 9, which can melt the microporous filter membrane when working. The heating part 9 can adopt the existing heating structure such as electric heating wire. In this embodiment, the electric heating wire is taken as an example. The electric heating wire is connected with the electric wire. The sampling part 2 and the soft rubber part 8 are provided with hole channels for the electric wire to pass through. The electric wire passes through the sampling part 2 and the soft rubber part 8 in turn and extends into the holding part 1 and is connected with the control circuit board in the holding part 1. The power supply interface of the control circuit board is connected with the plug through the electric wire. The control buttons 10 for controlling the working of the heating part 9 and the air suction and injection equipment are arranged on the holding part 1 and are connected with the control input end of the control circuit board. The control buttons 10 are arranged in multiple to control the heating part 9 and the air suction and injection equipment respectively. The control circuit board is provided with control chips and other electronic elements.

[0023] Specific use method: the microporous filter membrane cup in the patent with the patent announcement number CN106248978B, the cup body in the patent with the patent announcement number CN205981829U and other microporous filter membrane carriers can be used in cooperation with the present embodiment. The membrane carrier in the present embodiment includes a cup body 12, the upper and lower ends of the cup body 12 are open, and the lower end of the cup body 12 is provided with a microporous filter membrane 13. When sampling, the sampling part 2 is extended into the cup body 12 from top to bottom. In order to increase the sampling area, the sampling part 2 is provided with a counterbore 6 at one end of the heating part 9. In order to increase the stability of connection, a friction part can be arranged on the outer wall of the sampling part 2. The friction part can be made of silica gel, rubber and other materials. The friction part is in contact with the inner wall of the cup body 12. The counterbore 6 is located on the inner side of the heating part 9. The lower end opening of the flow channel 5 is located at the bottom of the counterbore 6. At the same time, since the thickness of the microporous filter membrane is relatively thin, in order to avoid excessive deformation of the microporous filter membrane 13 when vacuumizing, the counterbore 6 is provided with a support part 7. The support part 7 is provided with through holes at intervals. The support part 7 supports the upper end of the microporous filter membrane 13 to prevent the microporous filter membrane from being broken due to deformation.

[0024] Then the microporous filter membrane 13 is immersed in the specimen. The negative pressure generated by the air suction and injection equipment makes the specimen pass through the microporous filter membrane 13, the flow channel 5, the through hole and the exhaust pipe 4 into the sampling pipe 3 in turn. The cells, bacteria and other substances in the specimen are intercepted on the microporous filter membrane 13. After the specimen is absorbed, the detection personnel places the microporous filter membrane 13 on the slide 11 and then presses the heating part 9 flat on the upper end of the microporous filter membrane 13. With the working of the heating part 9 and the pressure applied by the detection personnel on the microporous filter membrane, the microporous filter membrane 13 is fixed on the slide. Then the cup body 12 is lifted up to separate the microporous filter membrane 13 from the cup body 12, so as to complete the preparation of the specimen.

[0025] After the preparation of the slice, the cup 12 is removed from the sampling part 2, and then the air suction and injection device injects air into the sampling tube 3 to push the sample out, so as to facilitate the subsequent preparation of the slice; before use, the slice preparation device can be cleaned by repeatedly injecting and pushing the disinfectant into the sampling tube 3.

[0026] In this embodiment, other structures and use methods are the same as those of the embodiment 1, but in this embodiment, the heating part 9 is not arranged at the lower end of the sampling part 2, and a sticky layer is arranged on the slide 11 used in cooperation, and the sticky layer can be glue, double-sided adhesive tape or the like meeting the hygiene requirements. During the preparation of the slice, the microporous filter membrane 13 is pressed on the sticky layer, so that the microporous filter membrane 13 is fixed on the slide 11 such as a glass slide, thereby completing the preparation of the slice. Figure 4

[0027] It should be noted that, in this document, the terms "comprising", "containing" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or further includes elements inherent to such a process, method, article or device.

[0028] The principle and implementation mode of the present application are described by using specific examples in this document, and the above examples are only used to help understand the method of the present application and its core idea. The above description is only the preferred implementation mode of the present application, and it should be pointed out that, due to the limited expression, there are infinite specific structures objectively, and for ordinary skilled persons in the technical field, some improvements, decorations or changes can be made without departing from the principle of the present application, and the above technical features can also be combined in a proper way; these improvements, decorations, changes or combinations, or the direct application of the concept and technical scheme of the present application to other occasions without improvement, should be regarded as the protection scope of the present application.​

Claims

1. A device for making smears suitable for membrane filtration smears, characterized in that, It comprises a holding part (1) and a sampling part (2), a sampling tube (3) is arranged in the holding part (1), the sampling tube (3) is in sealed communication with the sampling part, and the other end of the sampling tube (3) is connected with an air suction and injection device.

2. A device for making smears suitable for membrane filtration according to claim 1, characterized in that, One end of the sampling tube (3) is provided with a suction and discharge tube (4), and a flow channel (5) matched with the suction and discharge tube (4) is arranged on the sampling part (2) along the axial direction.

3. A device for making smears suitable for membrane filtration according to claim 2, characterized in that, A counterbore (6) is arranged at the lower end of the sampling part (2), and the lower end opening of the flow channel (5) is located at the bottom of the counterbore (6).

4. A device for making smears suitable for bacterial collection smears according to claim 3, characterized in that, A supporting part (7) is arranged in the counterbore (6), and through holes are arranged on the supporting part (7) at intervals.

5. The device for preparing a membrane bacterial smear according to claim 1, wherein A soft rubber part (8) is arranged at the joint of the sampling part (2) and the holding part (1), and through holes are arranged on the soft rubber part (8) and in communication with the flow channel (5).

6. A device for preparing a membrane bacterial smear according to any one of claims 1 to 5, characterized in that, A circle of heating parts (9) is arranged at the lower end of the sampling part (2).

7. A device for making smears suitable for membrane filtration according to claim 6, characterized in that, A control button (10) for controlling the working of the heating parts (9) is arranged on the holding part (1).

8. A device for making a film bacterial smear according to any one of claims 1 to 5, characterized in that, A supporting slide (11) is arranged on a slide (12) in matched use. A supporting slide (11) is arranged on a slide (12) in matched use.

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