Embryo cell operation port suction tube
By designing an oral pipette for embryonic cell manipulation, the problem of researchers having to remove their masks during operations was solved, enabling efficient cell manipulation without removing masks, thus ensuring the cleanliness of the experimental environment and the safety of personnel.
Patent Information
- Application Number
- CN202423246107.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2026-02-10
- Estimated Expiration
- 2034-12-26
AI Technical Summary
In mammalian in vitro embryo research, existing technologies require researchers to remove their masks when performing cell manipulations, which affects experimental safety and personnel health, and makes it difficult to maintain laboratory cleanliness for extended periods.
Design an oral pipette for embryonic cell manipulation, including an oral suction section, a connecting section, and an inhalation/exhalation section, equipped with a filter and an inclined tube, allowing researchers to operate without removing their masks. The airflow filter filters water vapor to ensure stable airflow.
It enables efficient cell manipulation without compromising the cleanliness of the experimental environment, ensuring experimental success rates and the safety of researchers.
Smart Images
Figure CN223892746U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of in vitro embryo research experimental technology, and relates to an oral pipette for embryonic cell manipulation. Background Technology
[0002] In in vitro embryonic research on mammals, researchers need to manipulate oocytes or embryonic cells (hereinafter referred to as "cells"), performing routine experimental procedures such as transfer between different culture media, post-IVF washing, and in vivo transplantation. To ensure cell contamination, high experimental success rates, and the safety of researchers, these experiments must be conducted in barrier environments or high-cleanliness environments, and researchers are required to wear masks during these procedures. Currently, researchers typically use self-made straight-through pipettes for cell manipulation, requiring the removal of masks before use. One end of the pipette is held in the mouth, and air is blown / inhaled into the pipette to control the transfer or cleaning of cells at the other end. Typically, cell manipulation without a mask takes at least ten minutes or more. Prolonged exposure to this can affect the safety control level required for experiments, thus conflicting with the relevant regulations of the experimental site. If large-scale and prolonged use of pipettes is employed, the entire laboratory environment may be unable to maintain the required safety level, leading to cell contamination, low experimental success rates, and in severe cases, potential health risks to researchers. Summary of the Invention
[0003] This invention proposes an oral pipette for embryonic cell manipulation, which allows researchers to perform various cell manipulations without removing their masks. This achieves a balance between maintaining a high level of experimental environment, efficiently achieving experimental objectives, and ensuring the safety of researchers, ultimately ensuring the safe operation of the laboratory.
[0004] The technical solution of this utility model to solve the above problems is:
[0005] This invention proposes an oral pipette for embryonic cell manipulation, which is unique in that:
[0006] It consists of three parts: the mouth-suction section at the front, the connecting section in the middle, and the inhalation / exhalation section at the rear.
[0007] The mouth-suction section includes a straight tube, an inclined tube, and a filter. One end of the straight tube is connected to one end of the filter, and the other end of the straight tube is connected to one end of the inclined tube. The connecting section includes a flexible tube, one end of which is connected to the other end of the filter. The inhalation / exhalation section includes a rigid connecting tube, one end of which is connected to the other end of the flexible tube, which is connected to a glass needle.
[0008] Furthermore, there is an included angle of 70° to 80° between the straight circular tube and the inclined tube.
[0009] Furthermore, the inclined tube is a flat oval tube, and its thickness gradually decreases from the end to the root.
[0010] Furthermore, the connection between the inclined tube and the straight tube is rounded.
[0011] Furthermore, the filter is used to filter water vapor in the airflow. It includes an upper cover and a lower cover, and the upper and lower covers form a cavity inside. The cavity is provided with a breathable filter material, which includes a spunbond nonwoven fabric layer and a meltblown nonwoven fabric layer. The spunbond nonwoven fabric is close to the upper cover and has a waterproof function, while the meltblown nonwoven fabric is made of polypropylene material with a high melt index fiber. It is an ultra-fine electrostatic fiber cloth that can effectively use electrostatic adsorption of viral dust and droplets.
[0012] Furthermore, a rubber stopper is inserted into the outlet of the rigid connecting tube, and an insertion hole is provided in the center of the rubber stopper, into which a glass needle is inserted.
[0013] Furthermore, the upper cover and the lower cover are connected by threads.
[0014] Furthermore, the filter has connecting pipes on its upper and lower covers. The connecting pipe of the upper cover is connected to a straight round pipe, and the connecting pipe of the lower cover is connected to a flexible hose.
[0015] Furthermore, the straight circular tube connecting to the filter has a connecting end, the outer diameter of which is smaller than the outer diameter of the straight circular tube.
[0016] Furthermore, the connecting tubes of the upper cover and the lower cover are respectively inserted into a straight round tube and a flexible tube.
[0017] Advantages of this utility model:
[0018] The embryonic cell manipulation mouth pipette provided by this utility model has a simple overall structure. Researchers can use the embryonic cell manipulation mouth pipette to perform various cell operations without removing their masks, thus achieving a balance between maintaining a high level of experimental environment, efficiently achieving experimental objectives, and ensuring the safety of researchers themselves. Attached Figure Description
[0019] Figure 1 This is a structural diagram of the oral pipette used for embryonic cell manipulation;
[0020] Figure 2 yes Figure 1 Enlarged view of point A in the middle.
[0021] In the diagram, 1. Straight round tube; 2. Filter; 3. Flexible tube; 4. Rigid connecting tube; 5. Rubber stopper; 6. Glass needle; 7. Inclined tube; 8. Top cover; 9. Bottom cover; 10. Breathable filter material. Detailed Implementation
[0022] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model. Therefore, the following detailed description of the embodiments of this utility model provided in the accompanying drawings is not intended to limit the scope of the claimed utility model, but merely to represent selected embodiments of this utility model.
[0023] See Figure 1 An embryonic cell manipulation mouth pipette consists of three parts: a front mouth pipette section, a middle connecting section, and a rear suction / extraction section.
[0024] The mouth-suction section includes a straight circular tube 1, an inclined tube 7, and a filter 2. One end of the straight circular tube 1 is connected to one end of the filter 2, and the other end of the straight circular tube 1 is connected to one end of the inclined tube 7. There is an angle of less than 90° between the straight circular tube 1 and the inclined tube 7. The connecting section includes a flexible tube 3, which is a rubber tube. One end of the flexible tube 3 is connected to the other end of the filter 2. The inhalation / exhalation section includes a rigid connecting tube 4. One end of the rigid connecting tube 4 is connected to the other end of the flexible tube 3, and the other end is connected to a rubber stopper 5. The rubber stopper 5 has an insertion hole in its center, into which a glass needle 6 is inserted.
[0025] In in vitro embryonic research on mammals, researchers need to perform routine experimental procedures such as transferring oocytes or embryonic cells between different culture media, washing after IVF, and in vivo transplantation. During specific cell manipulation experiments, the mask does not need to be removed. The three parts—the front mouthpiece, the middle connecting section, and the rear inhalation / exhalation section—are connected. The mouthpiece is inserted from under the mask and hung in the mouth. Researchers hold one end of the inclined tube 7 in their mouth and blow / inhale into the mouthpiece. The airflow passes sequentially through the inclined tube 7, the straight round tube 1, the filter 2, the flexible tube 3, the rigid connecting tube 4, and the glass needle 6, thereby controlling the entry, exit, transfer, washing, or transplantation of cells at the other end of the mouthpiece. Typically, during a complete cell experiment, water vapor in the airflow enters the filter 2, which filters the water vapor. This prevents saliva and water vapor from clogging the straw, ensuring a stable airflow during experiments. Researchers can use embryonic cells to manipulate various cells through the straw without removing their masks, achieving a balance between maintaining a highly stable experimental environment, efficiently achieving experimental objectives, and ensuring the safety of researchers.
[0026] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 1 The straight tube and the inclined tube have an included angle of 70° to 80°, allowing the inclined tube to hang in the mouth. Furthermore, the connection between the inclined tube and the straight tube is rounded, which helps to stabilize airflow at the bend and prevents it from falling off during breathing.
[0027] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 1 The inclined tube 7 is a flat round tube that is placed in the operator's mouth to increase comfort. Its thickness gradually decreases from the end to the root, so there is no need to worry about the inclined tube 7 slipping out of the mouth. This allows the operator to use both hands to perform other operations.
[0028] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 2 The filter 2 is used to filter water vapor in the airflow. It includes an upper cover 8 and a lower cover 9, which are connected by threads. The upper cover 8 and lower cover 9 form a cavity inside, and a breathable filter material 10 is provided inside the cavity. The breathable filter material 10 includes a spunbond nonwoven fabric layer and a meltblown nonwoven fabric layer. The spunbond nonwoven fabric is located near the upper cover and has water-blocking properties, while the meltblown nonwoven fabric is made of polypropylene material with a high melt index fiber. It is an ultra-fine electrostatic fiber cloth that can effectively utilize electrostatic adsorption of viral dust and droplets.
[0029] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 1 The filter 2 has connecting pipes on its upper cover 8 and lower cover 9. The connecting pipes of the upper cover 9 and lower cover 10 are respectively inserted into the straight round pipe 1 and the flexible hose 3.
[0030] In a preferred embodiment of this utility model, the straight circular tube 1 is provided with a connecting end at one end connected to the filter 2. The outer diameter of the connecting end is smaller than the outer diameter of the straight circular tube 1, making it easy for the flexible tube 3 to be fitted onto the connecting end. The flexible tube 3 is approximately 15-20cm long, 7mm in diameter, and 5mm in inner diameter, which ensures stable airflow.
[0031] The straight tube 1 and the inclined tube 7 are made of PPSU material, which can withstand high-temperature sterilization and can be reused. The rubber stopper 5 has a small hole in the middle, which can stably support the glass needle and achieve a seal when the glass needle is inserted. The glass capillary is a conventional consumable product, and the glass needle can be drawn under an alcohol lamp. The inner diameter of the tip is large enough to draw in mouse or rat embryonic cells.
[0032] The above description is merely an embodiment of this utility model and is not intended to limit the scope of protection of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related system fields, are similarly included within the scope of protection of this utility model.
Claims
1. An oral pipette for embryonic cell manipulation, characterized in that: It consists of three parts: the mouth-suction section at the front, the connecting section in the middle, and the inhalation / exhalation section at the rear. The mouth suction section includes a straight tube (1), an inclined tube (7) and a filter (2). One end of the straight tube (1) is connected to one end of the filter (2), and the other end of the straight tube (1) is connected to one end of the inclined tube (7). There is an angle of less than 90° between the straight tube (1) and the inclined tube (7). The connecting section includes a hose (3), one end of which is connected to the other end of the filter (2). The inhalation / exhalation section includes a rigid connecting tube (4), one end of which is connected to the other end of the flexible tube (3), and the other end is connected to a glass needle (6).
2. The oral pipette for embryonic cell manipulation according to claim 1, characterized in that: There is an angle of 70° to 80° between the straight tube (1) and the inclined tube (7).
3. The oral pipette for embryonic cell manipulation according to claim 2, characterized in that: The inclined tube (7) is a flat round tube, and its thickness gradually decreases from the end to the root.
4. The oral pipette for embryonic cell manipulation according to claim 3, characterized in that: The connection between the inclined tube (7) and the straight tube (1) is rounded.
5. The oral pipette for embryonic cell manipulation according to claim 4, characterized in that: A rubber stopper (5) is inserted into the outlet of the rigid connecting tube (4). The rubber stopper (5) has an insertion hole in the center, and a glass needle (6) is inserted into the insertion hole.
6. An oral pipette for embryonic cell manipulation according to any one of claims 1-5, characterized in that: The filter (2) is used to filter water vapor in the airflow. It includes an upper cover (9) and a lower cover (10). The upper cover (9) and the lower cover (10) form a cavity inside. The cavity is provided with a breathable filter material (11). The breathable filter material (11) includes a spunbond nonwoven fabric layer and a meltblown nonwoven fabric layer.
7. The oral pipette for embryonic cell manipulation according to claim 6, characterized in that: The upper cover (9) and the lower cover (10) are connected by threads.
8. The oral pipette for embryonic cell manipulation according to claim 7, characterized in that: The filter (2) has connecting pipes on its upper cover (9) and lower cover (10). The connecting pipe of the upper cover (9) is connected to the straight round pipe (1), and the connecting pipe of the lower cover (10) is connected to the flexible hose (3).
9. The oral pipette for embryonic cell manipulation according to claim 8, characterized in that: The straight circular tube (1) is connected to the filter (2) at one end, and the outer diameter of the connecting end is smaller than the outer diameter of the straight circular tube (1).
10. The oral pipette for embryonic cell manipulation according to claim 9, characterized in that: The connecting pipes of the upper cover (9) and the lower cover (10) are respectively inserted into the straight round pipe (1) and the flexible hose (3).