Liquid supplementing device for cell culture

Abstract

The utility model relates to the technical field of cell culture, and discloses a liquid supplementing device for cell culture, which comprises a culture device, according to the culture device, a bottle opening is formed in the top of a culture bottle; the box body is arranged above the culture bottle; the supporting plate is arranged at the bottom of the box body; the liquid inlet is formed in the top of the box body; the liquid supplementing opening is formed in the bottom of the box body; the valve is arranged at the bottom of the box body and is in butt joint with the bottle opening; according to the liquid supplementing device, the box body filled with the supplementing liquid is placed above the culture bottle, then the liquid supplementing opening in the bottom of the box body is in butt joint with the bottle opening, the box body is placed in an incubator, when the culture liquid needs to be supplemented, the valve on the liquid supplementing opening is opened, then the liquid enters the culture bottle from the liquid supplementing opening through the bottle opening, and the liquid supplementing is convenient and fast; the device can be well matched with a circular culture bottle, is low in use cost, can be matched with existing culture bottles, and is high in practicability.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a fluid replenishment device for cell culture. Background Technology

[0002] Cell culture refers to a method of enabling organisms to survive, grow, reproduce, and maintain their main structures and functions by simulating the in vivo environment (sterility, suitable temperature, pH, and certain nutritional conditions) outside the body. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technique. Whether for bioengineering as a whole or for biological cloning technology, cell culture is an indispensable process; cell culture itself is the large-scale cloning of cells. Cell culture technology can cultivate a single cell into a large number of simple single cells or minimally differentiated multicellular organisms, which is an essential step in cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used technique in cell biology research. Through cell culture, large numbers of cells can be obtained, and these cells can be used to study cell signal transduction, cellular synthesis and metabolism, cell growth and proliferation, etc.

[0003] When cells are cultured in traditional culture containers, it is often necessary to replenish the cells with nutrient solution. The traditional method is to manually add the liquid using a dropper. Some containers have been modified to add a replenishment device, eliminating the need for manual replenishment. However, this method increases equipment costs and the traditional culture containers are discarded, resulting in underutilization and waste. Therefore, we propose a replenishment device based on a circular bottle container. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a fluid replenishment device for cell culture, which offers the advantage of convenient fluid replenishment and solves the problems of cumbersome operation or high cost of traditional fluid replenishment devices.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, the present invention provides the following technical solution: a fluid replenishment device for cell culture, comprising a culture device; the culture device comprising: a bottle opening at the top of a culture flask; a box body disposed above the culture flask; a support plate disposed at the bottom of the box body; a fluid inlet disposed at the top of the box body; a fluid replenishment port disposed at the bottom of the box body; and a valve disposed at the bottom of the box body and connected to the bottle opening.

[0008] In some embodiments, the box body has a semi-cylindrical structure, and the liquid inlet is located at the center of the box body.

[0009] In some embodiments, an observation window is provided on the outer wall of the box.

[0010] In some embodiments, the culture apparatus is provided with an auxiliary component, the auxiliary component including: a fixing plate disposed on the outer side wall of the culture flask; and a rubber ring disposed on the inner wall of the fixing plate.

[0011] In some embodiments, the fixing plate and the support plate are threaded together.

[0012] In some embodiments, the outer walls of the liquid inlet and the bottle opening are provided with vent holes.

[0013] In some embodiments, a placement base that mates with the fluid inlet is provided below the support plate.

[0014] In some embodiments, the interior of the placement base is provided with absorbent cotton pads.

[0015] (III) Beneficial Effects

[0016] Compared with the prior art, the present invention provides a fluid replenishment device for cell culture, which has the following beneficial effects:

[0017] This replenishment device involves placing a box filled with replenishment solution on top of a culture flask, then connecting the replenishment port at the bottom of the box to the flask opening, and placing it in an incubator. When replenishment of culture solution is needed, the valve on the replenishment port is opened, and the liquid enters the culture flask through the replenishment port and the flask opening. The replenishment is convenient and quick, fits well with round culture flasks, has low operating costs, and can be used in conjunction with existing culture media, making it highly practical. Attached Figure Description

[0018] Figure 1 This utility model is three-dimensional. Figure 1 ;

[0019] Figure 2 This utility model is three-dimensional. Figure 2 ;

[0020] Figure 3 This is a cross-sectional view of the fixing plate of this utility model.

[0021] In the picture:

[0022] 1. Culture apparatus; 11. Culture flask; 12. Flask mouth; 13. Box body; 14. Support plate; 15. Liquid inlet; 16. Liquid replenishment port; 17. Valve; 18. Observation window;

[0023] 2. Auxiliary components; 21. Fixing plate; 22. Rubber ring; 23. Ventilation hole; 24. Placement base; 25. Absorbent cotton sheet. Detailed Implementation

[0024] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0025] It should be noted that all directional indications in the embodiments of this application are only used to explain the relative positional relationship and movement of each component in a specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0026] In this application, unless otherwise expressly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0027] Furthermore, the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. If the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed in this application.

[0028] In related technologies, the traditional method of replenishment is to manually add liquid using a dropper. Some containers have been modified to add liquid replenishment devices, eliminating the need for manual replenishment. However, this method increases equipment costs, and traditional culture containers are discarded, resulting in underutilization and waste.

[0029] To address some of the problems in the related technologies, this application provides a liquid replenishment device for cell culture. When needed, a container 13 is connected to the bottle opening 12 above the culture flask 11, and the valve 17 is manually controlled to replenish the liquid.

[0030] This application is described below with reference to the accompanying drawings and specific embodiments:

[0031] Combination Figures 1-3 This application provides a fluid replenishment device for cell culture, including a culture device 1; the culture device 1 includes: a culture flask 11 with a bottle mouth 12 at the top; a box body 13 disposed above the culture flask 11; a support plate 14 disposed at the bottom of the box body 13; a liquid inlet 15 disposed at the top of the box body 13; a fluid replenishment port 16 disposed at the bottom of the box body 13; and a valve 17 disposed at the bottom of the box body 13 and connected to the bottle mouth 12.

[0032] When using it, first put the cells and culture medium into the culture flask 11, and place the box 13 filled with replenishment medium on top of the culture flask 11. Then, connect the replenishment port 16 at the bottom of the box 13 with the bottle mouth 12 and put it into the incubator. When it is necessary to replenish the culture medium, open the valve 17 on the replenishment port 16, and then the liquid enters the culture flask 11 from the replenishment port 16 through the bottle mouth 12. The liquid replenishment is convenient and quick, and the box 13 can also act as the bottle cap of the culture flask 11.

[0033] In some embodiments, the housing 13 has a semi-cylindrical structure, and the liquid inlet 16 is located at the center of the housing 13. The housing 13 is configured as a semi-cylindrical structure, with the valve 17 extending out for easy rotation operation.

[0034] In some embodiments, an observation window 18 is provided on the outer side wall of the housing 13. The observation window 18 is for convenient observation of the liquid inside the housing 13, and then liquid is added from the liquid inlet 15 at the top.

[0035] In some embodiments, the culture device 1 is provided with an auxiliary component 2, which includes: a fixing plate 21 disposed on the outer side wall of the culture flask 11; and a rubber ring 22 disposed on the inner wall of the fixing plate 21. The fixing plate 21 is fitted onto the outer side wall of the culture flask 11 by the rubber ring 22, and is used to limit the position of the box body 13 and the support plate 14.

[0036] In some embodiments, the fixing plate 21 and the support plate 14 are threadedly connected. The threaded connection between the fixing plate 21 and the support plate 14 increases the stability of the support plate 14 and the housing 13.

[0037] In some embodiments, the outer walls of the liquid inlet 16 and the bottle mouth 12 are provided with vent holes 23. The vent holes 23 are for the convenience of the box body 13 replacing the cap with vent holes.

[0038] In some embodiments, a placement base 24 is provided below the support plate 14 to accommodate the liquid inlet 16. When not in use, the box 13 stands upright in the placement base 24 via the support plate 14 to prevent residual liquid from dripping from the liquid inlet 16.

[0039] In some embodiments, the interior of the placement base 24 is provided with absorbent cotton pads 25. The absorbent cotton pads 25 are for facilitating the absorption of residual liquid dripping from the replenishment port 16.

[0040] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," and "some examples" indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. In addition, those skilled in the art can combine and integrate the different embodiments or examples described in this specification.

[0041] Furthermore, the technical solutions of the various embodiments can be combined with each other, but only if they are based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0042] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A fluid replenishment device for cell culture, characterized in that: Includes a culture device (1); the culture device (1) includes: A culture flask (11) with a bottle mouth (12) at the top; The box body (13) is positioned above the culture flask (11); A support plate (14) is disposed at the bottom of the box body (13); The liquid inlet (15) is located on the top of the box body (13); A liquid replenishment port (16) is located at the bottom of the box body (13); A valve (17) is located at the bottom of the box (13) and is connected to the bottle opening (12).

2. The fluid replenishment device for cell culture according to claim 1, characterized in that: The box body (13) has a semi-cylindrical structure, and the liquid inlet (16) is located at the center of the box body (13).

3. The fluid replenishment device for cell culture according to claim 1, characterized in that: The outer wall of the box (13) is provided with an observation window (18).

4. The fluid replenishment device for cell culture according to claim 1, characterized in that: The culture device (1) is provided with an auxiliary component (2), which includes: A fixing plate (21) is disposed on the outer side wall of the culture flask (11); A rubber ring (22) is disposed on the inner wall of the fixing plate (21).

5. A fluid replenishment device for cell culture according to claim 4, characterized in that: The fixing plate (21) and the support plate (14) are threaded together.

6. The fluid replenishment device for cell culture according to claim 1, characterized in that: Ventilation holes (23) are provided on the outer walls of the liquid inlet (16) and the bottle mouth (12).

7. The fluid replenishment device for cell culture according to claim 1, characterized in that: Below the support plate (14) is a placement base (24) that matches the liquid replenishment port (16).

8. A fluid replenishment device for cell culture according to claim 7, characterized in that: The interior of the placement base (24) is provided with absorbent cotton pads (25).

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