Coliform group single-tube quantitative detection device

Abstract

The utility model relates to the technical field of bacterium detection equipment, and particularly discloses a coliform group single-tube quantitative detection device, which comprises a culture tube, a detection tube, a reducer tube, a piston and a silica gel foam plug, the surface of the detection tube is coated with a color-bacterium concentration relation table, a liquid discharge tube is arranged in the tube wall of the culture tube in a penetrating manner, and the liquid discharge tube is connected with the silica gel foam plug. A valve is connected to the portion, outside the culture tube, of the liquid discharging tube, the reducer tube comprises a small-diameter section, a first large-diameter section and a second large-diameter section, the first large-diameter section and the second large-diameter section are connected to the two ends of the small-diameter section respectively, the first large-diameter section is connected to the top of the culture tube in a sealed mode, and a silica gel foam plug is clamped in the second large-diameter section. The piston is connected into the small-diameter section in a sealed and sliding mode, the top of the piston is connected with a drawing rod, the drawing rod penetrates through the silica gel foam plug, and the culture tube achieves the effect of facilitating sampling under the condition that ventilation and bacterium isolation are guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model relates to bacterial detection equipment technical field especially relates to coliform bacteria single pipe quantitative detection device. BACKGROUND

[0002] CN206902150U discloses a kind of coliform bacteria single pipe quantitative detection device, it is set by setting liquid drain pipe of inverted "U" in the lower part of culture tube, sampling is facilitated, and it is inserted into breathable silica gel plug in the top of culture tube, it can effectively ventilate, and prevent outside microorganism into culture tube.But applicant finds in actual use, to effectively isolate outside microorganism, it is not possible to open too large breathable hole on silica gel plug, the best choice is to use foamed silica gel foam as plug, but the plug of foamed silica gel foam material can only slowly ventilate, which leads to in the case where silica gel plug is not removed, culture tube is maintained pressure and outside balance, cannot be quickly drained by liquid drain pipe, and since liquid drain pipe is inverted "U" type, even if silica gel plug is removed, it is also difficult to drain from liquid drain pipe, which loses the meaning of setting liquid drain pipe in the bottom of culture tube, and does not reach the effect of facilitating sampling. UTILITY MODEL CONTENT

[0003] To solve the problems mentioned in the background art, the purpose of the utility model is to provide coliform bacteria single pipe quantitative detection device.

[0004] To achieve the above target, the technical scheme of the utility model is as follows:

[0005] Coliform bacteria single pipe quantitative detection device, including culture tube, detection tube, variable diameter pipe, piston and silica gel foam plug, the surface of detection tube is coated with color-bacteria concentration relationship table, the wall of culture tube is provided with liquid drain pipe, the liquid drain pipe is sealingly connected with the wall of culture tube, the port of liquid drain pipe inside culture tube is close to the bottom of culture tube, the valve is connected on the liquid drain pipe outside culture tube, the variable diameter pipe includes a small diameter section and first large diameter section and second large diameter section connected at both ends of small diameter section, the first large diameter section is sealingly connected at the top of culture tube, the second large diameter section is provided with silica gel foam plug, the silica gel foam plug is spaced apart from the top opening of small diameter section, the distance between silica gel foam plug and the top opening of small diameter section is greater than the length of piston, the piston is sealingly and slidingly connected in small diameter section, the top of piston is connected with pull rod, the pull rod penetrates silica gel foam plug.

[0006] Further, the middle part of the inner wall of the second large diameter section is provided with flange, the top of the second large diameter section is threadedly connected with gland with open middle part, and the silica gel foam plug is clamped between the gland and the flange.

[0007] Further, the liquid drain pipe is inverted "U" type.

[0008] The utility model discloses the beneficial effect is: cultivate bacteria, pull the pull rod, and the piston is placed in the second big diameter section, oxygen can enter the culture tube through silica gel foam plug at this moment, and silica gel foam plug can isolate microorganism outside again, when needing detection, open the valve, and make the piston insert small diameter section after pushing down continuously, and the culture tube generates positive pressure, can effectively discharge the bacteria liquid by the liquid discharge pipe, under the condition of guaranteeing ventilation, isolation bacteria, really reached the effect of convenient sampling. BRIEF DESCRIPTION OF DRAWINGS

[0009] Fig. 1 It is the front view of the utility model embodiment;

[0010] Fig. 2 It is the section view of the culture tube in the utility model embodiment.

[0011] The drawing number explanation is: 1, culture tube, 11, liquid discharge pipe, 12, valve, 2, detection tube, 21, color-bacteria concentration relation table, 3, variable diameter pipe, 31, small diameter section, 32, first big diameter section, 33, second big diameter section, 331, flange, 4, piston, 41, pull rod, 5, silica gel foam plug, 6, gland. DETAILED DESCRIPTION

[0012] The technical scheme in the utility model embodiments will be described clearly and completely below in combination with the drawings in the utility model embodiments, and obviously, the described embodiments only are a part of the embodiments of the utility model, not all the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by the ordinary skill in the art belong to the protection scope of the utility model.

[0013] For example, Figs. 1-2As shown, the single-tube quantitative detection device for coliform bacteria comprises a culture tube 1, a detection tube 2, a variable-diameter tube 3, a piston 4 and a silica gel foam plug 5, the detection tube 2 is made of transparent material, preferably glass, the surface of the detection tube 2 is coated with a color-bacteria concentration relationship table 21, a liquid discharge tube 11 is arranged in the wall of the culture tube 1, the liquid discharge tube 11 is sealingly connected with the wall of the culture tube 1, the port of the liquid discharge tube 11 inside the culture tube 1 is arranged close to the bottom of the culture tube 1, a valve 12 is connected with the liquid discharge tube 11 outside the culture tube 1, preferably, the liquid discharge tube 11 is arranged in an inverted "U" shape, the variable-diameter tube 3 comprises a small-diameter section 31 and a first large-diameter section 32 and a second large-diameter section 33 connected at both ends of the small-diameter section 31, the first large-diameter section 32 is threadedly connected at the top of the culture tube 1, the second large-diameter section 33 is clamped with the silica gel foam plug 5, the silica gel foam plug 5 is arranged at intervals with the top opening of the small-diameter section 31, the interval between the silica gel foam plug 5 and the top opening of the small-diameter section 31 is greater than the length of the piston 4, the piston 4 is sealingly and slidingly connected in the small-diameter section 31, the top of the piston 4 is connected with a pull rod 41, the pull rod 41 penetrates through the silica gel foam plug 5, when the piston 4 is away from the small-diameter section 31, the silica gel foam plug 5 can ensure that the pull rod 41 does not sink.

[0014] In actual use, a proper amount of LST culture medium is loaded into the culture tube 1, the valve 12 is opened so that the culture medium can enter the liquid discharge tube 11 inside the culture tube 1, then the valve 12 is closed, the first large-diameter section 32 of the variable-diameter tube 3 is installed at the top of the culture tube 1, the culture tube 1 is high-pressure steam sterilized for standby, then the variable-diameter tube 3 is removed, the sample to be detected is added into the culture tube 1, and then the first large-diameter section 32 of the variable-diameter tube 3 is installed on the culture tube 1 for culture.

[0015] In the embodiment, the middle part of the inner wall of the second large-diameter section 33 is provided with a flange 331, the top of the second large-diameter section 33 is threadedly connected with a cover 6 with an open middle part, the silica gel foam plug 5 is clamped between the cover 6 and the flange 331, which is reasonable in design and facilitates disassembly and assembly of the silica gel foam plug 5.

[0016] The above merely describes the preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A single-tube quantitative detection device for coliform bacteria, comprising a culture tube (1) and a detection tube (2), wherein the surface of the detection tube (2) is coated with a color-bacterial concentration relationship table (21), a drain tube (11) is inserted through the inner wall of the culture tube (1), the drain tube (11) is sealed to the inner wall of the culture tube (1), the port of the drain tube (11) inside the culture tube (1) is located near the bottom of the culture tube (1), and a valve (12) is connected to the drain tube (11) outside the culture tube (1), characterized in that, The detection device further comprises a variable-diameter tube (3), a piston (4) and a silica gel foam plug (5), the variable-diameter tube (3) comprises a small-diameter section (31) and first and second large-diameter sections (32, 33) connected to both ends of the small-diameter section (31) respectively, the first large-diameter section (32) is sealingly connected to the top of the culture tube (1), the second large-diameter section (33) is provided with the silica gel foam plug (5) clamped therein, the silica gel foam plug (5) is spaced apart from the top opening of the small-diameter section (31), the distance between the silica gel foam plug (5) and the top opening of the small-diameter section (31) is greater than the length of the piston (4), the piston (4) is sealingly and slidingly connected in the small-diameter section (31), the top of the piston (4) is connected with a pull rod (41), and the pull rod (41) penetrates through the silica gel foam plug (5).

2. The coliform bacteria single-tube quantitative detection device according to claim 1, characterized in that, The middle part of the inner wall of the second large-diameter section (33) is provided with a flange (331), the top of the second large-diameter section (33) is threadedly connected with a middle-opened gland (6), and the silica gel foam plug (5) is clamped between the gland (6) and the flange (331).

3. The coliform bacteria single-tube quantitative detection device according to claim 1, characterized in that, The liquid drainage tube (11) is arranged in an inverted "U" shape.

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