Chromatographic column for purifying pepsin

Abstract

The utility model discloses a chromatographic column for purifying pepsin, which comprises a chromatographic column body for separating and purifying pepsin, and a supporting mechanism for keeping the stable state of the chromatographic column body is arranged on the chromatographic column body; the supporting mechanism comprises a limiting assembly for limiting the middle part of the chromatographic column body and a lifting part for supporting which are arranged up and down, and an adjusting part for connection is arranged between the limiting assembly and the lifting part; through cooperative use of the limiting assembly, the lifting part, the adjusting part and the bearing part, the chromatographic column body can be supported, so that the chromatographic column body is kept in a stable state to ensure that operation in pepsin purification related procedures is easy to carry out, the supporting height of the chromatographic column body can be adjusted, and the working efficiency is improved. Extraction devices with different heights can be arranged below the chromatographic column body to carry out extraction operation, so that the application range of the chromatographic column for pepsin purification is widened.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, and in particular to a chromatography column for purifying pepsin. Background Technology

[0002] The chromatography column is the core component of gel chromatography, typically made of glass or plexiglass. It's a chromatographic method that separates components in a sample mixture based on their different partition coefficients in the stationary and mobile phases.

[0003] A patent document with publication number CN222468132U discloses a sand-free core-reinforced, replaceable chromatography column. By setting up a chromatography column body and a reinforcing sleeve, it solves the problems of easy breakage of the piston part and outlet tube of the chromatography column, limited application range, and low separation efficiency. Steel wire reinforcement components are added to the reinforcing sleeve, the collection port, and the transfer tube at the bottom of the chromatography column to prevent the lower part of the chromatography column to the upper part of the outlet tube from breaking due to high temperature or external force when chromatography of overheated substances. The chromatography column does not contain a sand core, so as to broaden the experimental range. The bottom outlet tube is designed as a separate replaceable part, which facilitates real-time replacement and thus extends the service life of the chromatography column. By setting up the chromatography column body and the reinforcing sleeve, the problem of inconvenience in changing the direction of the switch handle of the piston at the bottom of the chromatography column is solved. The rotating sleeve is supported on the worktable by the support legs, and the support plate is supported on the worktable by the support legs through the rotating sleeve, so that the reinforcing sleeve and the chromatography column body are well supported, and the reinforcing sleeve and the chromatography column body can rotate relative to each other with the rotating sleeve, changing the position of the PTFE three-way piston, making the PTFE three-way piston easier to switch on and off.

[0004] Although the aforementioned sand-free core-reinforced replaceable chromatography column can solve the corresponding technical problems, the fixed height of its support legs results in a fixed distance between the outlet tube and the operating table. This makes it difficult to adjust the distance between the outlet tube and the operating table according to actual usage needs, making it difficult to place devices of different heights under the outlet tube for material extraction operations. Consequently, this reduces the applicability of the sand-free core-reinforced replaceable chromatography column.

[0005] Therefore, a chromatography column for the purification of pepsin is proposed. Utility Model Content

[0006] The purpose of this invention is to provide a chromatography column for purifying pepsin, which solves the problems mentioned in the background art.

[0007] To achieve the above objectives, the main technical solutions adopted by this utility model include:

[0008] A chromatography column for purifying pepsin, comprising:

[0009] The chromatography column body used for the separation and purification of pepsin is equipped with a support mechanism to maintain the stability of the chromatography column body.

[0010] The support mechanism includes a limiting component for limiting the middle part of the chromatography column body and a lifting component for support, which are arranged vertically. An adjusting component is provided between the limiting component and the lifting component for connection. The adjusting component is provided with a supporting component for auxiliary support of the bottom of the chromatography column body. The adjusting component can adjust the distance between the lifting component and the supporting component.

[0011] As a preferred technical solution, the chromatography column body includes a chromatography column body, the top end of the chromatography column body is connected to a grinding ring, the bottom end of the chromatography column body is connected to an outlet tube, and a PTFE piston is installed on the outlet tube.

[0012] As a preferred technical solution, the limiting component includes an upper ring sleeved on the middle part of the surface of the chromatography column, and two symmetrically arranged clamping members are provided on the upper ring, forming a clamping space between the two clamping members, and the chromatography column is located in the clamping space.

[0013] As a preferred technical solution, the clamping member includes a stud threaded through the upper ring, and a clamping plate is rotatably connected to one end of the stud facing the chromatography column, with the chromatography column located between the two clamping plates.

[0014] As a preferred technical solution, a guide rod is fixedly connected to the outer surface of the clamping plate and arranged parallel to the stud, with the end of the guide rod away from the clamping plate extending movably through to the outer side of the upper ring.

[0015] As a preferred technical solution, the lifting component includes a lower ring located below the guide rod, and three support legs arranged in a ring and equidistantly are fixedly connected to the lower ring. The bottom end of the liquid outlet pipe extends through to the bottom of the lower ring, and the adjusting component is located between the upper ring and the lower ring.

[0016] As a preferred technical solution, the adjusting component includes a support rod fixedly connected to the bottom of the upper ring. The support rods are three in a ring and are evenly distributed. The bottom end of the support rod is fixedly connected to an outer tube. The inner cavity of the outer tube is provided with an inner rod. The bottom end of one of the inner rods is rotatably connected to the lower ring, and the bottom ends of the other two inner rods are fixedly connected to the lower ring.

[0017] As a preferred technical solution, one of the inner rods has an external thread on its outer surface and an internal thread on the inner wall of the corresponding outer tube. The inner rod is threadedly connected to the corresponding outer tube through the external thread and the internal thread. The other two inner rods are slidably connected to the corresponding outer tubes. The support member is located between the three outer tubes.

[0018] As a preferred technical solution, the support member includes a ring fixedly connected between three outer tubes, the inner diameter of the top of the ring is larger than the inner diameter of its bottom, and the surface of the ring is provided with a through groove for inserting a liquid supply pipe.

[0019] As a preferred technical solution, rubber pads are fixedly connected to the inner walls of both the support ring and the clamping plate, and the inner wall of the rubber pads is in contact with the surface of the chromatography column.

[0020] This utility model has at least the following beneficial effects:

[0021] This application, through the combined use of limiting components, lifting components, adjusting components, and supporting components, can not only support the chromatography column body, keeping it in a stable state to ensure easy operation in pepsin purification-related processes, but also adjust the support height of the chromatography column body to allow extraction devices of different heights to be placed below the chromatography column body for extraction operations, thereby helping to improve the applicability of the chromatography column for pepsin purification; at the same time, the chromatography column body and the support mechanism are detachably connected, so that the chromatography column body can be removed for cleaning or replacement. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of the chromatography column for purifying pepsin according to this invention;

[0023] Figure 2 This is an exploded view of the structure of the chromatography column for purifying pepsin according to this invention;

[0024] Figure 3 This is a schematic diagram of the limiting component of the chromatography column for purifying pepsin according to this invention.

[0025] In the diagram: 100, chromatography column body; 110, chromatography column body; 120, frosted ring; 130, outlet tube; 140, PTFE piston; 200, support mechanism; 210, limiting assembly; 211, upper ring; 212, clamping component; 2121, stud; 2122, clamping plate; 2123, guide rod; 220, lifting component; 221, lower ring; 222, support leg; 230, adjusting component; 231, support rod; 232, outer tube; 233, inner rod; 234, knob; 240, support component; 241, support ring; 242, through groove; 250, rubber pad. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figures 1-3 An embodiment of this utility model provides a chromatography column for purifying pepsin, including a chromatography column body 100 for separating and purifying pepsin and a support mechanism 200 for maintaining the stability of the chromatography column body 100. The support mechanism 200 is disposed on the chromatography column body 100. The support mechanism 200 includes a limiting component 210 for limiting the middle part of the chromatography column body 100 and a lifting component 220 for support, which are arranged vertically. An adjusting component 230 is provided between the limiting component 210 and the lifting component 220 for connection. The adjusting component 230 is provided with a supporting component 240 for auxiliary support of the bottom of the chromatography column body 100. The adjusting component 230 can adjust the distance between the lifting component 220 and the supporting component 240.

[0028] The chromatography column body 100 includes a chromatography column 110, with a grinding ring 120 connected to the top of the chromatography column 110 and an outlet tube 130 connected to the bottom of the chromatography column 110. A PTFE stopcock 140 is installed on the outlet tube 130. Pepsin solution is injected into the chromatography column 110 through the grinding ring 120, and then a dissolving solution or neutral salt is injected into the chromatography column 110 through the grinding ring 120, so that the neutral salt and pepsin solution are fully mixed. When the PTFE stopcock 140 is opened, the purified pepsin solution can be extracted and analyzed through the outlet tube 130.

[0029] The limiting component 210 includes an upper ring 211 sleeved on the middle part of the surface of the chromatography column 110. The upper ring 211 is provided with two symmetrically arranged clamping members 212, and a clamping space is formed between the two clamping members 212. The chromatography column 110 is located in the clamping space.

[0030] The clamping member 212 includes a stud 2121 that is horizontally threaded through the upper ring 211. The end of the stud 2121 facing the chromatography column 110 is rotatably connected to a V-shaped clamping plate 2122 via a bearing. The chromatography column 110 is located between the two clamping plates 2122. The limiting component 210 can clamp and fix the chromatography column 110, so that the chromatography column 110 can be stably placed in the limiting component 210.

[0031] The outer surface of the clamping plate 2122 is fixedly connected to a guide rod 2123 arranged parallel to the stud 2121. The end of the guide rod 2123 away from the clamping plate 2122 extends through to the outside of the upper ring 211. When the clamping plate 2122 moves, it can drive the guide rod 2123 to move at the penetration point of the upper ring 211, so as to guide the clamping plate 2122 and enable the clamping plate 2122 to move horizontally stably, preventing the clamping plate 2122 from rotating synchronously with the stud 2121, thereby ensuring the subsequent clamping and fixing operation of the chromatography column 110 by the clamping plate 2122.

[0032] The lifting component 220 includes a lower ring 221 located below the guide rod 2123. Three support legs 222 are fixedly connected to the lower ring 221 at an angle and are distributed in a ring. The bottom end of the liquid outlet pipe 130 extends to the lower ring 221. The adjusting component 230 is located between the upper ring 211 and the lower ring 221. Through the coordinated use of the lifting component 220, the adjusting component 230 and the limiting component 210, the operation in the pepsin purification process can be easily carried out.

[0033] The adjusting component 230 includes a support rod 231 fixedly connected to the bottom of the upper ring 211. Three support rods 231 are evenly distributed in a ring. An outer tube 232 is fixedly connected to the bottom end of the support rod 231. An inner rod 233 is provided through the inner cavity of the outer tube 232. The bottom end of one inner rod 233 is rotatably connected to the lower ring 221 through a bearing. The bottom ends of the other two inner rods 233 are fixedly connected to the lower ring 221. The adjusting component 230 can realize the connection operation between the limiting component 210 and the lifting component 220, and can also adjust the distance between the lifting component 220 and the supporting component 240.

[0034] One of the inner rods 233 has an external thread on its outer surface, and the corresponding outer tube 232 has an internal thread on its inner wall. The inner rod 233 is threaded to the corresponding outer tube 232 through the external and internal threads. The surface of the inner rod 233 with external threads is fixedly connected to a knob 234 for easy rotation. The other two inner rods 233 are slidably connected to the corresponding outer tubes 232. The support member 240 is located between the three outer tubes 232. By rotating the knob 234, the inner rod 233 with external threads can be driven to rotate. Under the action of the threads, the inner rod 233 can be moved up or down in the inner cavity of the outer tube 232, thereby adjusting the distance between the lifting member 220 and the support member 240, and thus adjusting the height of the bottom end of the liquid outlet pipe 130 from the operating table, so that devices of different heights can be placed under the liquid outlet pipe 130 for extraction operations.

[0035] The support member 240 includes a ring 241 fixedly connected between the three outer tubes 232. The inner diameter of the top of the ring 241 is larger than the inner diameter of its bottom. The ring 241 can support the inverted conical part at the bottom of the chromatography column 110, which helps to improve the support stability of the support mechanism 200 on the chromatography column body 100. The surface of the ring 241 is provided with a through groove 242 for the liquid outlet pipe 130 to be inserted. The through groove 242 can facilitate the liquid outlet pipe 130 to be placed in the ring 241 from the side, avoiding the difficulty of inserting the PTFE piston 140 from top to bottom into the support member 240 due to its protruding structure.

[0036] Rubber pads 250 are fixedly connected to the inner walls of the support ring 241 and the clamping plate 2122. The inner wall of the rubber pad 250 is in contact with the surface of the chromatography column 110. The rubber pad 250 not only prevents the support ring 241 and the clamping plate 2122 from directly contacting the surface of the chromatography column 110 and causing wear, but also increases the friction between the support ring 241 and the chromatography column 110, and between the clamping plate 2122 and the chromatography column 110, which helps to improve the stability of the chromatography column body 100.

[0037] The working principle of this utility model is as follows: The chromatography column body 100 is passed through the upper ring 211 from top to bottom. When the outlet pipe 130 approaches the support ring 241, the chromatography column body 110 is tilted so that the outlet pipe 130 moves to the outside of the support ring 241. The chromatography column body 110 is then moved downward so that the PTFE piston 140 is located below the support ring 241. At this time, the chromatography column body 110 is straightened so that the outlet pipe 130 is placed in the support ring 241 through the through groove 242. The outlet pipe 130 extends to the bottom of the lower ring 221 until the inverted conical part at the bottom of the chromatography column body 110 contacts the rubber pad 250 on the support ring 241. The stud 2121 is rotated, and under the action of the thread, the clamping plate 2122 and the guide rod 2123 move synchronously towards the chromatography column body 110. Move the column until the rubber pad 250 on the clamp 2122 contacts the surface of the chromatography column 110 to complete the fixing of the chromatography column body 100. The support mechanism 200 can stably support the chromatography column body 100 on the operating table. Rotate the knob 234, which drives the corresponding inner rod 233 to rotate. Under the action of the thread, the inner rod 233 drives the outer tube 232 to move up or down on its surface. The outer tube 232 drives the support rod 231, the support member 240 and the limiting component 210 to move. The support member 240 and the limiting component 210 drive the chromatography column body 100 to move synchronously, thereby adjusting the distance between the bottom end of the liquid outlet tube 130 and the operating table, so that the extraction device at the same height can be placed below the liquid outlet tube 130.

[0038] All parts not described in this utility model are the same as or can be implemented using existing technology. Although embodiments of this utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of this utility model, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A chromatography column for the purification of pepsin, characterized in that, The utility model relates to a chromatography column body (100) for separating and purifying pepsin, which is provided with a support mechanism (200) for keeping the chromatography column body (100) in a stable state. The support mechanism (200) comprises a limiting assembly (210) arranged above and below for limiting the middle part of the chromatography column body (100) and a lifting piece (220) for supporting, and an adjusting piece (230) for connecting is arranged between the limiting assembly (210) and the lifting piece (220), the adjusting piece (230) is provided with a supporting piece (240) for assisting the bottom of the chromatography column body (100), and the distance between the lifting piece (220) and the supporting piece (240) can be adjusted by the adjusting piece (230). The chromatography column body (100) comprises a chromatography column body (110), the top end of the chromatography column body (110) is communicated with a frosted ring (120), the bottom end of the chromatography column body (110) is communicated with a liquid outlet pipe (130), and a tetrafluoroethylene piston (140) is installed on the liquid outlet pipe (130).

2. The pepsin purification chromatography column according to claim 1, characterized in that: The limiting assembly (210) comprises an upper ring (211) sleeved on the middle part of the surface of the chromatography column body (110), the upper ring (211) is provided with two symmetrical clamping pieces (212), a clamping space is formed between the two clamping pieces (212), and the chromatography column body (110) is located in the clamping space.

3. The pepsin purification chromatography column according to claim 2, characterized in that: The clamping piece (212) comprises a threaded stud (2121) penetrating the upper ring (211), one end of the threaded stud (2121) towards the chromatography column body (110) is rotationally connected with a clamping plate (2122), and the chromatography column body (110) is located between the two clamping plates (2122).

4. The pepsin purification chromatography column according to claim 3, characterized in that: The outer surface of the clamping plate (2122) is fixedly connected with a guide rod (2123) arranged in parallel with the threaded stud (2121), and one end of the guide rod (2123) away from the clamping plate (2122) is movably penetrated to the outside of the upper ring (211).

5. The pepsin purification chromatography column according to claim 4, characterized in that: The lifting piece (220) comprises a lower ring (221) located below the guide rod (2123), the lower ring (221) is fixedly connected with three support legs (222) arranged in a ring shape at equal intervals, the bottom end of the liquid outlet pipe (130) penetrates to the lower side of the lower ring (221), and the adjusting piece (230) is located between the upper ring (211) and the lower ring (221).

6. The pepsin purification chromatography column according to claim 5, characterized in that: The adjusting piece (230) comprises a support rod (231) fixedly connected to the bottom of the upper ring (211), the support rod (231) is arranged in a ring shape at equal intervals and has three, the bottom end of the support rod (231) is fixedly connected with an outer tube (232), the inner cavity of the outer tube (232) is penetrated with an inner rod (233), one end of the inner rod (233) is rotationally connected with the lower ring (221), and the other two ends of the inner rod (233) are fixedly connected with the lower ring (221).

7. The pepsin purification chromatography column according to claim 6, characterized in that: ​ 8. The pepsin purification chromatography column according to claim 7, characterized in that: An outer surface of one of the inner rods (233) is provided with external threads, an inner wall surface of a corresponding outer tube (232) is provided with internal threads, the inner rod (233) is threadedly connected in the corresponding outer tube (232) through the external threads and the internal threads, the other two inner rods (233) are slidingly connected in the corresponding outer tubes (232), and the supporting member (240) is arranged between the three outer tubes (232).

9. The pepsin purification chromatography column according to claim 7, characterized in that: The supporting member (240) comprises a supporting ring (241) fixedly connected between the three outer tubes (232), an inner diameter of a top of the supporting ring (241) is larger than an inner diameter of a bottom of the supporting ring (241), and a surface of the supporting ring (241) is provided with a through groove (242) for placing the outlet pipe (130).

10. The pepsin purification chromatography column according to claim 9, characterized in that: The supporting ring (241) and an inner wall surface of the clamping plate (2122) are both fixedly connected with rubber pads (250), and inner wall surfaces of the rubber pads (250) are in contact with a surface of the chromatographic column body (110).

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