Cell co-culture plate
By incorporating a partition assembly and a removable mesh within the culture plate, the problems of culture plate contamination and insufficient material exchange were solved, enabling the co-culture of multiple cell types and improving experimental efficiency.
Patent Information
- Application Number
- CN202520400542.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2026-02-17
- Estimated Expiration
- 2035-03-10
AI Technical Summary
Existing culture plates are prone to contamination when small chambers are added, and the exchange of substances between cells is insufficient, which cannot meet the complex environmental requirements of co-culturing multiple cell types.
Design a cell co-culture plate that divides the culture tank into multiple culture chambers by setting a partition assembly inside the culture tank, and uses a detachable mesh to realize the exchange of substances between cells. The mesh cooperates with the spacers through slots to adapt to the growth needs of different cells.
This method enables the co-culture of multiple cell types, avoids contamination in small chambers, improves the efficiency of cell material exchange, and enhances the flexibility and accuracy of experiments.
Smart Images
Figure CN223921422U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model discloses a cell biology technical field, especially relates to a cell co-culture plate. BACKGROUND
[0002] Cell co-culture is a technique that grows two or more different cell types in the same culture system under controlled conditions. This technique allows cells to interact and signal each other while sharing liquid media or growth factors. Cell co-culture is a very effective method for studying cell-cell interactions and can reveal answers to many important biological questions. During cell co-culture, the culture plate provides a platform for cell growth and proliferation.
[0003] When using a culture plate for cell co-culture, a chamber structure is added to allow two different types of cells to coexist in the same culture system without direct contact. However, the addition of the chamber structure has the following drawbacks:
[0004] (1) The chamber surface is not properly treated, and the cells in the chamber have poor adhesion and other environmental conditions, which are not in the best state for cells.
[0005] (2) Using the chamber can only co-culture two types of cells, and more chambers cannot be added to the culture tank, so the culture plate cannot meet the complex environment of more cell co-culture.
[0006] (3) During cell co-culture, the growth state, morphological changes, and interactions between cells need to be observed regularly, so the chamber needs to be frequently removed, which can easily cause contamination. UTILITY MODEL CONTENT
[0007] The utility model aims at solving the problem of contamination caused by the addition of a chamber in the existing culture plate and insufficient exchange of substances between cells, and proposes a cell co-culture plate.
[0008] To achieve the above purpose, the utility model adopts the following technology: a cell co-culture plate, comprising a plate body, the top of the plate body is recessed downward to form a culture tank, the inside of the culture tank is divided into at least two culture chambers along the circumferential direction by a partition assembly integrally formed with it, and the material exchange between cells is realized by a partition net assembled on the partition assembly between adjacent culture chambers.
[0009] As a further description of the above technical solution: the partition assembly comprises a center column arranged along the axis of the culture tank, the center column is provided with a spacing sheet with a window, and the partition net is detachably installed on the spacing sheet through a clamping groove.
[0010] As a further description of the above technical solution: the number of the spacing sheets is two and they are arranged in a straight line in the culture tank.
[0011] As a further description of the above technical solution: the number of the spacing sheets is three and they are arranged in a circle around the axis of the culture tank.
[0012] As a further description of the above technical solution: the number of the spacing sheets is four and they are arranged in a cross in the culture tank.
[0013] As a further description of the above technical solution: the top of the spacing net is provided with a mounting section for the snap-fit assembly, and the mounting section is provided with a rubber ring matched with the snap groove.
[0014] As a further description of the above technical solution: the number of the culture tanks is several and they are arranged in an array on the plate body.
[0015] In summary, due to the adoption of the above technical solution, the beneficial effects of the present application are:
[0016] (1) The culture tank is divided into at least two co-culture chambers by the partition assembly, so as to realize the co-culture of two or more than two cells and meet the complex environment of more cell co-culture; if the co-cultured cells are all adherent cells or are suitable, the cell secretions can be fully exchanged through the snap groove with the hollowed-out position of the added spacing net; if the co-cultured cells have suspended cells or other suitable conditions, the spacing net is added, and the substances of the co-cultured cells can also be exchanged through the spacing net.
[0017] (2) Without adding the chamber, the problem of frequent taking of the chamber during the culture operation and easy contamination of the chamber can be avoided. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 An overall structure schematic diagram according to an embodiment of the present application is shown;
[0019] Figure 2 A partial cross-sectional schematic diagram according to an embodiment of the present application is shown;
[0020] Figure 3 A partial structure schematic diagram according to an embodiment of the present application is shown;
[0021] Figure 4 A structure schematic diagram of the spacing net according to an embodiment of the present application is shown;
[0022] Figure 5 A structure schematic diagram of the partition assembly of the first embodiment according to an embodiment of the present application is shown;
[0023] Figure 6 A schematic diagram of the partition assembly according to Embodiment 2 of the present invention is shown;
[0024] Figure 7 A schematic diagram of the partition assembly according to Embodiment 3 of the present invention is shown.
[0025] Legend:
[0026] 1. Plate body; 2. Culture tank; 3. Partition assembly; 31. Central column; 32. Spacer; 33. Slot; 4. Partition mesh; 41. Installation section; 42. Rubber ring. Detailed Implementation
[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.
[0028] Example 1
[0029] Reference Figures 1-5 This embodiment provides a cell co-culture plate, including a plate body 1. The top of the plate body 1 is recessed downward to form a culture tank 2. The interior of the culture tank 2 is divided into at least two culture chambers along the circumferential direction by a partition assembly 3 integrally formed with it. The adjacent culture chambers are connected by a mesh 4 mounted on the partition assembly 3 to achieve intercellular material exchange. The partition assembly 3 includes a central column 31 arranged along the axis of the culture tank 2. The central column 31 is provided with a spacer 32 with a window. The mesh 4 is detachably mounted on the spacer 32 through a slot 33.
[0030] In this invention, the culture tank 2 is divided into at least two culture chambers by spacers 32 integrally formed with the central column 31. Selected cells are seeded into different culture chambers, and the distance between the bottom of the slot 33 and the bottom of the culture tank 2 is at least 0.5 mm. If the co-cultured cells are all adherent cells or in a similar condition, the spacer 4 is not required, and the secretions of each cell can be fully exchanged through the slots 33 where the spacer 4 is located. If the co-cultured cells include suspension cells or other similar conditions, the spacer 4 is installed, and the substances of the co-cultured cells can also be exchanged through the spacer 4.
[0031] The spacers 32 are three in number and arranged in a circle around the axis of the culture tank 2. The three spacers 32 divide the culture tank 2 into three culture chambers, so that two or three types of cells can be co-cultured.
[0032] Specifically, the top of the spacer 4 is provided with an installation section 41 for its snap-fit assembly. The installation section 41 is provided with a rubber ring 42 that mates with the slot 33. Through the design of the slot 33 on the spacer 32, together with the installation section 41 and the rubber ring 42 on the spacer 4, the spacer 4 can be easily disassembled and assembled, thus facilitating the replacement of the spacer 4. Different types of cells have different requirements for the microenvironment, including the exchange rate of oxygen, nutrients, and waste, as well as the interaction between cells. By replacing the spacer 4 with different pore sizes, these microenvironmental conditions can be adjusted to meet the growth and differentiation needs of different types of cells.
[0033] Specifically, there are several culture tanks 2 arranged in an array on the plate 1. By arranging multiple culture tanks 2 in an array, multiple experimental conditions can be tested at the same time, thereby improving experimental efficiency. Moreover, each experimental condition can be repeated multiple times, thereby reducing experimental errors and improving the accuracy of experimental results.
[0034] Example 2
[0035] like Figures 1-4 , Figure 6 As shown, unlike Embodiment 1, there are two spacers 32 arranged in a straight line in the culture tank 2. The straight-line arrangement of the spacers 32 divides the culture tank 2 into two culture chambers, thereby enabling the co-culture of two types of cells.
[0036] Example 3
[0037] like Figures 1-4 , Figure 7 As shown, unlike Embodiment 1 and Embodiment 2, there are four spacers 32 arranged in a cross shape in the culture tank 2. The cross-shaped spacers 32 divide the culture tank 2 into four culture chambers, thereby enabling the co-culture of two, three, or four types of cells.
[0038] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.
Claims
1. A cell co-culture plate, characterized in that, The plate (1) is recessed at the top to form a culture tank (2). The interior of the culture tank (2) is divided into at least two culture chambers along the circumferential direction by a partition assembly (3) integrally formed therewith. The adjacent culture chambers exchange substances between cells through a mesh (4) assembled on the partition assembly (3).
2. The cell co-culture plate according to claim 1, characterized in that, The partition assembly (3) includes a central column (31) arranged along the axis of the culture tank (2), and a spacer (32) with a window is provided on the central column (31). The partition net (4) is detachably installed on the spacer (32) through a slot (33).
3. A cell co-culture plate according to claim 2, characterized in that, The number of spacers (32) is two and they are arranged in a straight line in the culture tank (2).
4. A cell co-culture plate according to claim 2, characterized in that, The number of spacers (32) is three and they are arranged in a circle around the axis of the culture tank (2).
5. A cell co-culture plate according to claim 2, characterized in that, The number of spacers (32) is four and they are arranged in a cross shape in the culture tank (2).
6. A cell co-culture plate according to claim 3, 4, or 5, characterized in that, The top of the partition (4) is provided with an installation section (41) for its snap-fit assembly, and the installation section (41) is provided with a rubber ring (42) that mates with the slot (33).
7. A cell co-culture plate according to claim 1, characterized in that, The number of culture tanks (2) is several and they are arranged in an array on the plate (1).