Chromatographic reagent card shell and chromatographic reagent card

Abstract

The utility model provides a chromatographic reagent card shell and a chromatographic reagent card. The chromatography reagent card shell comprises a lower cover and an upper cover, wherein the lower cover comprises a sample pad fitting part; the upper cover and the lower cover are detachably arranged in a covering manner, at least one of the upper cover and the lower cover is provided with a reinforcing rib, the upper cover comprises a sample adding hole and a flow guide groove, and the sample adding hole penetrates through the thickness direction of the upper cover; the flow guide groove is communicated with one side, facing the lower cover, of the sample adding hole, and orthographic projections of the opening side, facing the lower cover, of the sample adding hole and the opening side, facing the lower cover, of the flow guide groove on the lower cover are located in an area where the sample pad attaching part is located. Therefore, the strength of the chromatography reagent card shell can be improved, the risk that the performance of the reagent card is influenced by deformation of the chromatography test paper card in the application process is reduced, and the risk that the problems of no infiltration, no sample loading, liquid leakage and the like in the sample chromatography process can also be reduced.

Description

Technical Field

[0001] This application belongs to the field of reagent kits, and more specifically, this application relates to chromatography reagent cartridges and chromatography reagent cards. Background Technology

[0002] Chromatographic reagent cards typically consist of two parts: a test strip and a cartridge. The test strip usually includes a PVC base plate, upon which a sample pad, a marking pad, a nitrocellulose membrane, and an absorbent pad are sequentially glued. The cartridge is used to assemble and secure the test strip, enabling a single test. However, when testing whole blood samples, the viscous nature and complex composition of whole blood can lead to problems such as sample not penetrating, not being sampled, and leakage during chromatography. Furthermore, the cartridge is prone to deformation due to insufficient strength during reagent assembly, transportation, storage, and use, thus affecting reagent performance. Utility Model Content

[0003] In a first aspect of this application, a chromatography reagent cartridge is provided, comprising:

[0004] The lower cover includes a sample pad fitting portion; and

[0005] An upper cover and a lower cover are detachably fitted together, and at least one of the upper cover and the lower cover is provided with a reinforcing rib. The upper cover includes:

[0006] A sample dispensing hole, the sample dispensing hole extending through the thickness direction of the upper cover; and

[0007] The flow guide channel is connected to the side of the sample dispensing hole facing the lower cover. The orthographic projection of the opening side of the sample dispensing hole facing the lower cover and the opening side of the flow guide channel facing the lower cover on the lower cover is located in the area where the sample pad is attached.

[0008] The chromatography reagent cartridge of the first aspect of this application has at least the following technical effects: it can not only improve the strength of the chromatography reagent cartridge and reduce the risk that the performance of the chromatography test strip will be affected by deformation during its application; it can also reduce the risk of problems such as no seepage, no sample loading, and leakage during sample chromatography.

[0009] In some embodiments of this application, the flow channel extends along the length of the upper cover.

[0010] In some embodiments of this application, the guide groove is arranged symmetrically along the sample feeding hole.

[0011] In some embodiments of this application, the width of the sample dispensing hole facing the opening side of the lower cover and the width of the opening side of the guide groove are smaller than the width of the sample pad fitting portion.

[0012] In some embodiments of this application, the depth of the guide groove is 0.1mm-0.5mm.

[0013] In some embodiments of this application, the width of the opening side of the guide groove is equal to the width of the sample dispensing hole facing the opening side of the lower cover.

[0014] In some embodiments of this application, the reinforcing rib includes a first reinforcing rib and / or a second reinforcing rib, wherein the first reinforcing rib is disposed on the side of the upper cover facing the lower cover, and the second reinforcing rib is disposed on the side of the lower cover facing the upper cover.

[0015] In some embodiments of this application, the first reinforcing rib includes a first sub-reinforcing rib extending along the length direction of the upper cover and a second sub-reinforcing rib extending along the width direction of the upper cover.

[0016] In some embodiments of this application, the second reinforcing rib includes a third sub-reinforcing rib extending along the length direction of the lower cover and a fourth sub-reinforcing rib extending along the width direction of the lower cover.

[0017] In some embodiments of this application, the top cover includes at least two first sub-reinforcing ribs spaced apart along its width direction, each first sub-reinforcing rib being connected to a plurality of second sub-reinforcing ribs spaced apart along the length direction of the top cover, and at least a portion of the second sub-reinforcing ribs being connected to the first sub-reinforcing ribs and extending to the edge of the top cover.

[0018] In some embodiments of this application, the lower cover includes at least two third sub-reinforcing ribs spaced apart along its width direction, each of the third sub-reinforcing ribs being connected to a plurality of fourth sub-reinforcing ribs spaced apart along the length direction of the lower cover, and at least a portion of the fourth sub-reinforcing ribs being connected to the third sub-reinforcing ribs and extending to the edge of the lower cover.

[0019] In some embodiments of this application, the sample pad fitting portion is located between two adjacent third sub-reinforcing ribs.

[0020] In some embodiments of this application, the surface of the sample loading hole is a polished surface.

[0021] In some embodiments of this application, the surface roughness Ra of the sample loading orifice is 0.02 μm-0.1 μm.

[0022] In some embodiments of this application, the area of ​​the sample feeding hole facing the opening side of the lower cover is smaller than the area of ​​the sample feeding hole facing away from the opening side of the lower cover.

[0023] In some embodiments of this application, the upper cover has an information bar on the side opposite to the lower cover, and the information on the information bar is recessed and raised on the surface of the upper cover.

[0024] In some embodiments of this application, the lower cover has an information bar on the side opposite to the upper cover, and the information on the information bar is recessed and raised on the surface of the lower cover.

[0025] In some embodiments of this application, the length of the sample pad fitting portion is 10mm-14mm and the width is 3.4mm-3.6mm.

[0026] In some embodiments of this application, the length of the sample feeding hole away from the opening side of the lower cover is 8mm-12mm, and the width is 8mm-12mm.

[0027] In some embodiments of this application, the length of the sample feeding hole facing the opening side of the lower cover is 5mm-7.5mm, and the width is 2mm-3.5mm.

[0028] In some embodiments of this application, the information on the information bar is arranged in a concave shape.

[0029] In some embodiments of this application, the information bar is provided on the side of the upper cover away from the lower cover, and the height difference between the information on the information bar and the surface of the upper cover is 0.1mm-0.3mm.

[0030] In some embodiments of this application, the information bar is provided on the side of the lower cover away from the upper cover, and the height difference between the information on the information bar and the surface of the lower cover is 0.1mm-0.3mm.

[0031] In some embodiments of this application, the information on the information bar includes one or more of the following: text, symbols, graphic symbols, QR codes, and barcodes.

[0032] In some embodiments of this application, the lower cover includes a nitrocellulose membrane bonding portion; the upper cover includes a detection window that extends through the thickness direction of the upper cover, and the orthographic projection of the opening side of the detection window toward the lower cover onto the lower cover is located in the area where the nitrocellulose membrane bonding portion is located.

[0033] In some embodiments of this application, the distance from the center of the sample application hole to the center of the detection window is 20mm-28mm along the length of the top cover.

[0034] In some embodiments of this application, the upper cover further includes a pressure bone, which protrudes toward the lower cover and extends along the width direction of the upper cover; the lower cover includes the sample pad bonding portion, the marking pad bonding portion, the nitrocellulose membrane bonding portion and the absorbent pad bonding portion distributed sequentially along its length direction, with an overlapping area between adjacent bonding portions, and the orthographic projection of the pressure bone on the lower cover is located within the overlapping area.

[0035] In some embodiments of this application, the lower cover further includes a cushioning pad fitting portion located between the marking pad fitting portion and the nitrocellulose membrane fitting portion; the pressure bone includes a first pressure bone, the orthographic projection of the first pressure bone on the lower cover being located within the overlapping area of ​​the marking pad fitting portion and the cushioning pad fitting portion; and / or, the pressure bone includes a second pressure bone, the orthographic projection of the second pressure bone on the lower cover being located within the overlapping area of ​​the cushioning pad fitting portion and the nitrocellulose membrane fitting portion.

[0036] In some embodiments of this application, the upper cover further includes a first pressure pin, which protrudes toward the lower cover, and the orthographic projection of the first pressure pin on the lower cover is located in the area where the sample pad fitting portion is located.

[0037] In some embodiments of this application, the upper cover further includes a second pressure pin, which protrudes toward the lower cover; the lower cover further includes an absorbent pad fitting portion, and the orthographic projection of the second pressure pin on the lower cover is located in the area where the absorbent pad fitting portion is located.

[0038] In some embodiments of this application, the first pressure pin includes at least two sub-pressure pins spaced apart along the width direction of the upper cover.

[0039] In some embodiments of this application, the upper cover has multiple rows of second pressure pins on the side facing the lower cover. The multiple rows of second pressure pins are spaced apart along the length direction of the upper cover, and each row of second pressure pins includes at least two sub-pressure pins spaced apart along the width direction of the upper cover.

[0040] In some embodiments of this application, the lower cover includes at least one pair of first limiting pins, each pair of first limiting pins being disposed on both sides of the sample pad fitting portion along the width direction of the lower cover and defining the width of the sample pad fitting portion.

[0041] In some embodiments of this application, the lower cover includes an absorbent pad fitting portion and at least one pair of second limiting pins, each pair of second limiting pins being disposed on both sides of the absorbent pad fitting portion along the width direction of the lower cover and defining the width of the absorbent pad fitting portion.

[0042] In some embodiments of this application, the lower cover includes a plurality of pairs of the second limiting pins spaced apart along its length.

[0043] In some embodiments of this application, an opening groove is provided between the upper cover and the lower cover.

[0044] In some embodiments of this application, the upper cover has a first connecting portion on the side facing the lower cover, and the lower cover has a second connecting portion on the side facing the upper cover, and the second connecting portion is engaged with the first connecting portion.

[0045] In a second aspect of this application, a chromatography reagent card is provided, comprising a test strip and a chromatography reagent card casing as described in the first aspect of this application, wherein the test strip is sandwiched between an upper cover and a lower cover. This chromatography reagent card has good strength, and the risk of problems such as non-diffusion, sample failure, or leakage during chromatography is relatively low. Attached Figure Description

[0046] Figure 1 This is a schematic diagram of the structure of the upper cover facing the lower cover in one embodiment of this application.

[0047] Figure 2 This is a schematic diagram of the structure of the upper cover facing the lower cover in another embodiment of this application.

[0048] Figure 3 This is a schematic diagram of the structure of the upper cover facing the lower cover in another embodiment of this application.

[0049] Figure 4 This is a schematic diagram of the structure of the upper cover facing away from the lower cover in one embodiment of this application.

[0050] Figure 5 This is a side view of the top cover in one embodiment of this application.

[0051] Figure 6 This is a schematic diagram of the structure of the lower cover facing the upper cover in one embodiment of this application.

[0052] Figure 7 This is a schematic diagram of the structure of the lower cover facing away from the upper cover in one embodiment of this application.

[0053] Figure 8 This is a side view of the lower cover in one embodiment of this application.

[0054] Figure 9 This is a schematic diagram of the structure of the lower cover facing the upper cover in another embodiment of this application.

[0055] Figure 10 This is a schematic diagram of the structure of the lower cover facing the upper cover in another embodiment of this application.

[0056] Explanation of reference numerals in the attached figures:

[0057] 10-Lower cover; 11-Sample pad fitting part; 12-Nitrocellulose membrane fitting part; 13-Marking pad fitting part; 14-Absorbent pad fitting part; 15-Buffer pad fitting part; 16-First limiting pin; 17-Second limiting pin; 18-Second connecting part; 20-Upper cover; 21-Sample dispensing hole; 22-Guide groove; 23-Detection window; 24-Pressure bone; 24a-First pressure bone; 24b-Second pressure bone; 25-First pressure pin; 26-Second pressure pin; 27-First connecting part; 31-First reinforcing rib; 31a-First sub-reinforcing rib; 31b-Second sub-reinforcing rib; 32-Second reinforcing rib; 32a-Third sub-reinforcing rib; 32b-Fourth sub-reinforcing rib; 40-Information bar; 50-Opening groove; 60-Overlapping area. Detailed Implementation

[0058] The present application will be further described below with reference to specific embodiments. It should be understood that these specific embodiments are for illustrative purposes only and are not intended to limit the scope of the present application.

[0059] In this application, the following definitions and methods are provided to better define this application and to guide those skilled in the art in its practice. Unless otherwise stated, the terms are to be understood in accordance with their conventional usage by those skilled in the art.

[0060] As used herein, the term "comprising" should be interpreted as inclusive and open-ended, not exclusive. Specifically, when used in the specification and claims, the term "comprising" and its variations mean including the specified features, steps, or components. These terms should not be construed as excluding the presence of other features, steps, or components.

[0061] As used herein, the term “optional” means that the events or circumstances described below may, but are not required to, occur, including both when they occur and when they do not.

[0062] In this document, the terms "first," "second," "third," and "fourth" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first," "second," "third," or "fourth" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0063] Wherever a range of values ​​is given herein, the range includes its endpoints, as well as all individual integers and fractions within the range, and also includes each narrower range formed by all the various possible combinations of those endpoints and internal integers and fractions, to form a subgroup of a larger group of values ​​within the same extent as each of those narrower ranges is explicitly given.

[0064] In a first aspect of this application, a chromatography reagent cartridge is provided, with reference to Figures 1-8 The understanding includes: a lower cover 10 and an upper cover 20, the lower cover 10 including a sample pad fitting portion 11; the upper cover 20 and the lower cover 10 are detachably fitted together, and at least one of the upper cover 20 and the lower cover 10 is provided with a reinforcing rib; the upper cover 20 includes a sample feeding hole 21 and a guide groove 22, the sample feeding hole 21 extending through the thickness direction of the upper cover 20; the guide groove 22 communicates with the side of the sample feeding hole 21 facing the lower cover 10, and the orthographic projection of the opening side of the sample feeding hole 21 facing the lower cover 10 and the opening side of the guide groove 22 facing the lower cover 10 on the lower cover 10 is located in the area where the sample pad fitting portion 11 is located.

[0065] The chromatography reagent cartridge of the first aspect of this application has at least the following technical effects: by providing reinforcing ribs on at least one of the upper cover 20 and the lower cover 10, the strength of the chromatography reagent cartridge can be improved, reducing the risk that the performance of the chromatography test strip card will be affected by deformation during its application; in addition, by providing a flow guide groove 22 that communicates with the sample dispensing hole 21, and by ensuring that the orthogonal projection of the sample dispensing hole 21 and the flow guide groove 22 toward the opening side of the lower cover 10 is located in the area where the sample pad fitting part 11 is located, not only can the sample dispensing hole 21 and the flow guide groove 22 be fixed, but also the flow guide groove 22 can be fixed. The position and size of the groove 22 ensure that after the chromatographic reagent cartridge is assembled with the test strip, the sample application hole 21 and the flow channel 22 correspond to the sample pad of the test strip and the size is not larger than the size of the sample pad. It also achieves the purpose of flow guidance and liquid storage. Taking whole blood sample as an example, the flow channel 22 can promote the whole blood sample to fill the entire sample pad during the infiltration process, so that the sample pad can evenly intercept red blood cells and other blood components, and prevent the sample pad from affecting the chromatography effect due to local blockage. This can reduce the risk of problems such as no infiltration, no sample application, and leakage during sample chromatography.

[0066] Understandably, in chromatography reagent cards, to ensure smooth sample loading by moving the sample pad to the absorption pad and reducing the risk of leakage, the opening side of the sample dispensing hole facing the lower cover is designed to directly contact the sample pad, i.e., abut against the sample pad of the test strip. In this application, the sample dispensing hole 21 and the flow guide groove 22 on the side facing the lower cover 10 are also adapted to abut against the sample pad of the test strip. The groove wall of the flow guide groove 22, distributed circumferentially, can reduce the risk of leakage. That is, in the thickness direction of the chromatography reagent card, the depth of the sample pad contact portion 11 is greater than or equal to the distance between the opening side of the sample dispensing hole 21 and the flow guide groove 22 facing the lower cover 10 and the lower cover 10.

[0067] It is understandable that the upper cover 20 and the lower cover 10 can be sealed together by a closing mechanism. In addition, the sample pad contact portion 11 refers to the area where the lower cover 10 and the sample pad of the test strip are directly or indirectly attached after the test strip is assembled on the upper cover 20 and the lower cover 10.

[0068] In some embodiments of this application, reference is made to 1. Figure 2 or Figure 3 It is understood that the flow channel 22 can extend along the length of the upper cover 20. This further facilitates sample loading and reduces the risk of sample pad leakage.

[0069] In some embodiments of this application, reference is made to Figure 1 , Figure 2 or Figure 3 It is understood that, along the length of the top cover 20, the flow guide 22 can be located either on one side of the sample dispensing port 21 or on both sides of the sample dispensing port 21. Compared to having it located on one side of the sample dispensing port 21, having the flow guide 22 on both sides of the sample dispensing port 21, i.e., having a bidirectional flow guide, helps to further reduce the risk of problems such as non-percolation, sample not being dispensed, or leakage during sample chromatography.

[0070] In some embodiments of this application, reference is made to Figure 1 , Figure 2 or Figure 3 It is understood that the flow channel 22 can be symmetrically arranged along the sample dispensing port 21. For example, the flow channel 22 can be symmetrically arranged along the length and / or width direction of the sample dispensing port 21, or the flow channel 22 can be symmetrically arranged along the center of the sample dispensing port 21. This helps to further reduce the risk of problems such as non-percolation, sample failure, and leakage during sample chromatography.

[0071] In some embodiments of this application, reference is made to Figure 1 and Figure 9 It is understood that the width of the sample application hole 21 facing the opening of the lower cover 10 and the width of the flow guide groove 22 facing the opening can be smaller than the width of the sample pad fitting portion 11. This further fixes the position and size of the sample application hole 21 and the flow guide groove 22, ensuring that after the chromatography reagent cartridge is assembled with the test strip, the sample application hole 21 and the flow guide groove 22 correspond to the sample pad of the test strip, and their widths are smaller than the width of the sample pad, thereby further reducing the risk of leakage.

[0072] In some embodiments of this application, reference is made to Figure 1 , Figure 2 or Figure 3 It is understood that the width of the opening side of the guide channel 22 can be equal to the width of the opening side of the sample feeding hole 21 facing the lower cover 10, thereby further reducing the risk of leakage.

[0073] In some embodiments of this application, the depth of the guide channel 22 can be 0.1mm-0.5mm, for example, it can be 0.1mm, 0.12mm, 0.15mm, 0.18mm, 0.2mm, 0.22mm, 0.25mm, 0.28mm, 0.3mm, 0.32mm, 0.35mm, 0.38mm, 0.4mm, 0.42mm, 0.45mm, 0.48mm, 0.5mm, etc., or it can be any range of the above values. This helps to further reduce the risk of problems such as non-percolation, sample failure, and leakage during sample chromatography, resulting in a lower sample failure rate and leakage rate.

[0074] In some embodiments of this application, reference is made to Figure 1 and Figure 6 It is understood that the reinforcing ribs may include a first reinforcing rib 31 and / or a second reinforcing rib 32. The first reinforcing rib 31 may be located on the side of the upper cover 20 facing the lower cover 10, and the second reinforcing rib 32 may be located on the side of the lower cover 10 facing the upper cover 20. This not only improves the strength of the chromatography reagent card casing and reduces the risk of deformation of the chromatography test strip affecting the performance of the reagent card during application, but also simplifies the surface structure of the chromatography reagent card casing, facilitating the assembly, packaging, and testing of the chromatography reagent cards, such as enabling automated assembly, packaging, and on-machine testing of the chromatography reagent cards.

[0075] In some implementations, reference Figure 1 , Figure 2 or Figure 3 It is understood that the first reinforcing rib 31 may include a first sub-reinforcing rib 31a extending along the length direction of the upper cover 20 and a second sub-reinforcing rib 31b extending along the width direction of the upper cover 20. Thus, the strength of the upper cover 20 can be further improved by the longitudinal and transverse arrangement of the first sub-reinforcing ribs 31a and the second sub-reinforcing ribs 31b.

[0076] In some implementations, reference Figure 1 , Figure 2 or Figure 3 It is understood that the upper cover 20 may include at least two first sub-reinforcing ribs 31a spaced apart along its width direction. Each first sub-reinforcing rib 31a may be connected to a plurality of second sub-reinforcing ribs 31b spaced apart along the length direction of the upper cover 20, and at least some of the second sub-reinforcing ribs 31b are connected to the first sub-reinforcing ribs 31a and extend to the edge of the upper cover 20. This further enhances the strength of the upper cover 20.

[0077] In some implementations, reference Figure 6 , Figure 9 or Figure 10It is understood that the second reinforcing rib 32 may include a third sub-reinforcing rib 32a extending along the length direction of the lower cover 10 and a fourth sub-reinforcing rib 32b extending along the width direction of the lower cover 10. Thus, the strength of the lower cover 10 can be further improved by the longitudinal and transverse arrangement of the third sub-reinforcing rib 32a and the fourth sub-reinforcing rib 32b.

[0078] In some implementations, reference Figure 6 , Figure 9 or Figure 10 It is understood that the lower cover 10 may include at least two third sub-reinforcing ribs 32a spaced apart along its width direction. Each third sub-reinforcing rib 32a may be connected to a plurality of fourth sub-reinforcing ribs 32b spaced apart along the length direction of the lower cover 10, and at least some of the fourth sub-reinforcing ribs 32b are connected to the third sub-reinforcing ribs 32a and extend to the edge of the lower cover 10. Thus, the strength of the lower cover 10 can be further improved by the crisscrossing arrangement of the third sub-reinforcing ribs 32a and the fourth sub-reinforcing ribs 32b.

[0079] In some implementations, reference Figure 9 or Figure 10 It is understood that the sample pad fitting portion 11 can be located between two adjacent third sub-reinforcing ribs 32a. This can further reduce the risk that deformation of the chromatographic reagent, Cayin, will affect its performance.

[0080] In some embodiments of this application, the surface of the sample dispensing orifice 21 can be a polished surface. The smooth surface of the polished surface can reduce friction and facilitate the accurate flow of the sample into the bottom of the dispensing orifice to reach the sample pad.

[0081] In some embodiments of this application, the surface roughness Ra of the sample application orifice 21 can be 0.02 μm to 0.1 μm, for example, it can be 0.02 μm, 0.025 μm, 0.03 μm, 0.035 μm, 0.04 μm, 0.045 μm, 0.05 μm, 0.055 μm, 0.06 μm, 0.065 μm, 0.07 μm, 0.075 μm, 0.08 μm, 0.085 μm, 0.09 μm, 0.095 μm, or 0.1 μm, or a range of any of the above values. This reduces friction and facilitates accurate sample flow into the bottom of the orifice and onto the sample pad.

[0082] In some embodiments of this application, reference is made to Figure 4 It is understood that the area of ​​the sample dispensing hole 21 facing the opening of the lower cover 10 can be smaller than the area of ​​the sample dispensing hole 21 facing away from the opening of the lower cover 10. This reduces operator errors in sample dispensing. Optionally, the area of ​​the sample dispensing hole 21 can gradually decrease along the direction closer to the lower cover 10, at least a portion of the thickness of the upper cover 20.

[0083] In some embodiments of this application, the size of the sample application hole 21 facing the opening side of the lower cover 10 can be flexibly adjusted according to the size of the sample pad fitting part 11 or the size of the test strip sample pad to meet the position and size requirements of the sample application hole 21.

[0084] In some implementations, reference Figure 9 or Figure 10 It is understood that the length of the sample pad bonding portion 11 can be 10mm-14mm and the width can be 3.4mm-3.6mm. For example, the length of the sample pad bonding portion 11 can be 10mm, 10.5mm, 11mm, 11.5mm, 12mm, 12.5mm, 13mm, 13.5mm or 14mm, or any range of the above values; the width can be 3.4mm, 3.45mm, 3.5mm, 3.55mm or 3.6mm, or any range of the above values.

[0085] Furthermore, in some implementations, reference is made to... Figure 4 It is understood that the length of the sample feeding hole 21 away from the opening side of the lower cover 10 can be 8mm-12mm, and the width can be 8mm-12mm. For example, the length of the sample dispensing hole 21 away from the opening of the lower cover 10 can be 8mm, 8.25mm, 8.5mm, 8.75mm, 9mm, 9.25mm, 9.5mm, 9.75mm, 10mm, 10.25mm, 10.5mm, 10.75mm, 11mm, 11.25mm, 11.5mm, 11.75mm, or 12mm, or any range of the above values; the width can be 8mm, 8.25mm, 8.5mm, 8.75mm, 9mm, 9.25mm, 9.5mm, 9.75mm, 10mm, 10.25mm, 10.5mm, 10.75mm, 11mm, 11.25mm, 11.5mm, 11.75mm, or 12mm, or any range of the above values. This helps to further reduce operator errors in sample dispensing.

[0086] Furthermore, in some implementations, reference is made to... Figure 4It is understood that the length of the sample dispensing hole 21 facing the opening of the lower cover 10 can be 5mm-7.5mm, and the width can be 2mm-3.5mm. For example, the length of the sample dispensing hole 21 facing the opening of the lower cover 10 can be 5mm, 5.25mm, 5.5mm, 5.75mm, 6mm, 6.25mm, 6.5mm, 6.75mm, or 7mm, or any range of the above values; the width can be 8mm, 8.25mm, 8.5mm, 8.75mm, 9mm, 9.25mm, 9.5mm, 9.75mm, 10mm, 10.25mm, 10.5mm, 10.75mm, 11mm, 11.25mm, 11.5mm, 11.75mm, or 12mm, or any range of the above values. This helps to further reduce operator errors in sample dispensing, and lower the sample non-sample rate and leakage rate.

[0087] In some embodiments of this application, reference is made to Figure 4 It is understood that an information panel 40 can be provided on the side of the upper cover 20 opposite to the lower cover 10, and the information on the information panel 40 can be raised and recessed with the surface of the upper cover 20. In addition to meeting the requirements for gripping and anti-slip, chromatography reagent card cases usually need to display information such as QR codes, project names, and company logos. However, the remaining area of ​​the top cover of the chromatography reagent card case is limited after the sample dispensing port and detection window. By making the information on the information panel 40 raised and recessed with the surface of the upper cover 20, the anti-slip requirements for operators or instruments to grip the reagent card can be met while displaying information such as the company brand, thus achieving both functionality and saving space. Optionally, the height difference between the information on the information bar 40 and the surface of the top cover 20 can be 0.1mm-0.3mm, for example, 0.1mm, 0.12mm, 0.14mm, 0.16mm, 0.18mm, 0.2mm, 0.22mm, 0.24mm, 0.26mm, 0.28mm or 0.3mm, or any range of the above values. This not only meets the anti-slip requirements for gripping, but also takes into account the strength of the top cover and the flatness of the overall structure.

[0088] In some embodiments of this application, an information panel 40 may be provided on the side of the lower cover 10 opposite to the upper cover 20, and the information on the information panel 40 may be raised or lowered with respect to the surface of the lower cover 10. This allows for the display of company brand information while also meeting the anti-slip requirements of operators or instruments for gripping reagent cards, achieving both functionality and space saving. Optionally, the height difference between the information on the information panel 40 and the surface of the lower cover 10 can be 0.1mm-0.3mm, for example, 0.1mm, 0.12mm, 0.14mm, 0.16mm, 0.18mm, 0.2mm, 0.22mm, 0.24mm, 0.26mm, 0.28mm, or 0.3mm, or any range of the above values. This not only meets the anti-slip requirements for gripping but also ensures the strength of the lower cover and the flatness of the overall structure.

[0089] In some embodiments of this application, reference is made to Figure 4 It is understood that the information on information panel 40 may include, but is not limited to, one or more of the following: text, symbols, graphic logos, QR codes, and barcodes. For example, one or more of the following can be used to display or retrieve information such as project names and company logos.

[0090] In some embodiments of this application, the information on the information bar 40 may be concave. For example, see reference... Figure 4 It is understood that the information panel 40 can be located on the side of the upper cover 20 opposite to the lower cover 10, with the information recessed towards the lower cover 10. This satisfies the need for a non-slip grip and also ensures the flatness of the overall structure of the chromatography reagent card case, facilitating the assembly, packaging, and testing of the chromatography reagent cards. For example, it can facilitate automated assembly, packaging, and on-machine testing of the chromatography reagent cards.

[0091] In some embodiments of this application, reference is made to Figure 4 It is understood that the information panel 40 can be located on the side of the upper cover 20 opposite to the lower cover 10, and the information on the information panel 40 is concave, with the depth of the concavity of the information on the information panel 40 on the upper cover 20 being 0.1mm-0.3mm. This not only satisfies the need for grip and anti-slip but also ensures the flatness of the overall structure and the strength of the upper cover, while also facilitating the display of information on the information panel.

[0092] In some embodiments of this application, reference is made to Figure 1 and Figure 9It is understood that the lower cover 10 also includes a nitrocellulose membrane bonding portion 12; the upper cover 20 may include a detection window 23, which extends through the thickness direction of the upper cover 20, and the orthographic projection of the opening side of the detection window 23 facing the lower cover 10 onto the lower cover 10 is located within the area where the nitrocellulose membrane bonding portion 12 is located. This is more conducive to observing or presenting the detection results, such as facilitating the detection of fluorescence signals by fluorescence instruments.

[0093] In some embodiments, the distance from the center of the sample well 21 to the center of the detection window 23 along the length of the top cover 20 can be 20mm-28mm. For example, it can be 20mm, 20.5mm, 21mm, 21.5mm, 22mm, 22.5mm, 23mm, 23.5mm, 24mm, 24.5mm, 25mm, 25.5mm, 26mm, 26.5mm, 27mm, 27.5mm, or 28mm, or any range of the above values. In existing chromatography reagent cards, the distance from the center of the sample well to the center of the detection window is usually controlled at around 34cm. The setting adopted in this application can shorten the distance from the sample well to the nitrocellulose membrane portion of the test strip, and the amount of sample required during the detection process is also smaller. Taking whole blood samples as an example, a smaller sample amount can reduce the accumulation of red blood cells and other blood components in the sample pad, and improve the problem that the sample pad is blocked by a large accumulation of red blood cells and other blood components, which leads to the sample not seeping into the sample well and affecting the chromatography.

[0094] In some embodiments of this application, reference is made to Figure 1 , Figure 2 or Figure 3 It is understood that the upper cover 20 may also include a pressure rib 24, which may be protruding toward the lower cover 10 and extend along the width direction of the upper cover 20; see reference. Figure 9 It is understood that the lower cover 10 may include a sample pad bonding portion 11, a marker pad bonding portion 13, a nitrocellulose membrane bonding portion 12, and an absorbent pad bonding portion 14, which are sequentially distributed along its length. There is an overlap area 60 between adjacent bonding portions, and the orthogonal projection of the pressure plate 24 onto the lower cover 10 can be located within the overlap area 60. The upper cover, satisfying the above conditions, allows the pressure plate to contact and press against the overlapping areas of the components of the test strip (i.e., sample pad, marker pad, nitrocellulose membrane, absorbent pad, etc.) after the test strip is assembled into the chromatography reagent cartridge. This facilitates tight bonding of the components of the test strip (i.e., sample pad, marker pad, nitrocellulose membrane, absorbent pad, etc.), resulting in more uniform chromatography. It is understood that the marker pad bonding portion 13, the nitrocellulose membrane bonding portion 12, and the absorbent pad bonding portion 14 refer to the areas where the lower cover 10 directly or indirectly adheres to the marker pad, nitrocellulose membrane, and absorbent pad of the test strip after the test strip is assembled into the upper cover 20 and the lower cover 10.

[0095] In some implementations, reference Figure 10It is understood that the lower cover 10 may also include a buffer pad bonding portion 15, which may be located between the marker pad bonding portion 13 and the nitrocellulose membrane bonding portion 12. That is, the lower cover 10 may include a sample pad bonding portion 11, a marker pad bonding portion 13, a buffer pad bonding portion 15, a nitrocellulose membrane bonding portion 12, and an absorbent pad bonding portion 14, which are distributed sequentially along its length. It is understood that the buffer pad bonding portion 15 refers to the area where the lower cover 10 and the test strip buffer pad are directly or indirectly bonded after the upper cover 20 and the lower cover 10 are assembled with the test strip.

[0096] Further, refer to Figure 1-3 and Figure 10 It is understood that the pressure plate 24 may include a first pressure plate 24a. The orthographic projection of the first pressure plate 24a on the lower cover 10 may be located within the overlapping area of ​​the marking pad fitting portion 13 and the buffer pad fitting portion 15. This allows the first pressure plate to contact and press against the overlapping area of ​​the marking pad and buffer pad of the test strip after the test strip is assembled in the chromatography reagent cartridge, promoting more uniform chromatography. And / or,

[0097] Further, refer to Figure 1-3 and Figure 10 It is understood that the pressure bone 24 may include a second pressure bone 24b. The orthographic projection of the second pressure bone 24b on the lower cover 10 may be located in the overlapping area of ​​the buffer pad bonding portion 15 and the nitrocellulose membrane bonding portion 12. This allows the first pressure bone to contact and press against the overlapping area of ​​the test strip buffer pad and the nitrocellulose membrane after the test strip is assembled in the chromatography reagent cartridge, thus promoting more uniform chromatography.

[0098] In some embodiments of this application, reference is made to Figure 1-3 and Figure 9 It is understood that the upper cover 20 may also include a first pressing pin 25, which may protrude towards the lower cover 10. The orthogonal projection of the first pressing pin 25 on the lower cover 10 may be located in the area where the sample pad fitting portion 11 is located. The upper cover, satisfying the aforementioned conditions, allows the first pressing pin to contact and press against the sample pad of the test strip after the test strip is assembled in the chromatography reagent cartridge, thereby fixing the test strip and ensuring it adheres tightly to the lower cover. This facilitates the even and secure fixing of the test strip components to the lower cover of the cartridge.

[0099] Optionally, refer to Figure 1-3 and Figure 9 It is understood that the first pressure pin 25 may include at least two sub-pressure pins spaced apart along the width direction of the upper cover 20. This further facilitates the flat and secure fixing of the test strip components to the lower cover of the casing.

[0100] In some embodiments of this application, reference is made to Figure 1-3 and Figure 9It is understood that the upper cover 20 may also include a second pressing pin 26, which may protrude towards the lower cover 10; the lower cover 10 may also include an absorbent pad fitting portion 14, and the orthographic projection of the second pressing pin 26 on the lower cover 10 may be located within the area where the absorbent pad fitting portion 14 is located. The upper cover satisfying the above conditions allows the second pressing pin to contact and press against the absorbent pad of the test strip after the test strip is assembled in the chromatography reagent cartridge, thereby fixing the test strip and promoting its close contact with the lower cover. This facilitates the flat and fixed placement of the test strip components on the lower cover of the cartridge.

[0101] Optionally, refer to Figure 1-3 and Figure 9 It is understood that the upper cover 20 may have multiple rows of second pressure pins 26 on the side facing the lower cover 10. The multiple rows of second pressure pins 26 may be spaced apart along the length of the upper cover 20, and each row of second pressure pins 26 may include at least two sub-pressure pins spaced apart along the width of the upper cover 20. This further facilitates the flat fixing of each component of the test strip to the lower cover of the casing.

[0102] In some implementations, reference Figure 1 , Figure 2 or Figure 3 Understandably, the top cover 20 may include a row of first pressing studs 25 and two rows of second pressing studs 26. The first pressing studs 25 may include two sub-pressing studs spaced apart along the width direction of the top cover 20. The two rows of second pressing studs 26 may be spaced apart along the length direction of the top cover 20, and each row of second pressing studs 26 may include two sub-pressing studs spaced apart along the width direction of the top cover 20.

[0103] In some embodiments of this application, reference is made to Figure 9 or Figure 10 It is understood that the lower cover 10 may include at least one pair of first limiting pins 16, each pair of first limiting pins 16 being disposed on both sides of the sample pad adhesion portion 11 along the width direction of the lower cover 10 and defining the width of the sample pad adhesion portion 11. Thus, the distance between each pair of first limiting pins can be adjusted according to the width of the test strip, thereby achieving limitation on one side of the sample pad of the test strip in the width direction.

[0104] In some embodiments of this application, reference is made to Figure 9 or Figure 10 It is understood that the lower cover 10 may include an absorbent pad fitting portion 14 and at least one pair of second limiting pins 17. Each pair of second limiting pins 17 may be disposed on both sides of the absorbent pad fitting portion 17 along the width direction of the lower cover 10 and define the width of the absorbent pad fitting portion 17. Thus, the distance between each pair of second limiting pins can be adjusted according to the width of the test strip, thereby achieving limiting of the absorbent pad on one side of the test strip in the width direction. Optionally, the lower cover may include multiple pairs of second limiting pins 17 spaced apart along its length direction, thereby further improving the limiting effect on the absorbent pad on one side of the test strip in the width direction.

[0105] In some implementations, reference Figure 6 , Figure 9 or Figure 10 It is understood that the lower cover 10 can simultaneously have at least one pair of first limiting pins 16 and at least one pair of second limiting pins 17. This allows for the positioning of both ends of the test strip using the first and second limiting pins. Optionally, on the side of the lower cover 10 facing the upper cover 20, only the first limiting pins 16 and the second limiting pins 17 can be provided to limit the width of the test strip's contact portion. That is, the lower cover can use only the first and second limiting pins to limit the test strip through point contact. Compared to using surface contact to limit the test strip, using the first and second limiting pins on the lower cover through point contact provides better tolerance for the test strip's width and helps reduce the risk of liquid being diverted through the fixed position during chromatography.

[0106] In some implementations, reference Figure 6 , Figure 9 or Figure 10 It is understood that the lower cover 10 may include a pair of first limiting pins 16 and three pairs of second limiting pins 17, and the three pairs of second limiting pins 17 may be spaced apart along the length direction of the lower cover 10. This can further improve the limiting effect on the width direction of the test strip.

[0107] In some embodiments of this application, an opening groove 50 may be provided between the upper cover 20 and the lower cover 10. This facilitates R&D technicians in opening the cover to identify problems with the chromatography test strips.

[0108] In some embodiments of this application, the way the upper cover 20 and the lower cover 10 are detachably fitted is not particularly limited, and those skilled in the art can choose flexibly according to actual needs. For example, refer to... Figure 1 and Figure 6 Understandably, in some embodiments, the side of the upper cover 20 facing the lower cover 10 may be provided with a first connecting portion 27, and the side of the lower cover 10 facing the upper cover 20 may be provided with a second connecting portion 18, and the second connecting portion 18 and the first connecting portion 27 may be snapped together.

[0109] In a second aspect of this application, a chromatography reagent card is provided, comprising a test strip and a chromatography reagent card housing as described in the first aspect of this application, wherein the test strip is sandwiched between the upper and lower covers of the chromatography reagent card housing. This chromatography reagent card has good strength, and the risk of problems such as non-percolation, sample failure, or leakage during chromatography is relatively low.

[0110] In some embodiments of this application, reference is made to Figure 1-8It is understood that the chromatography reagent card includes the chromatography reagent card shell and reagent strip as described in the first aspect of this application. The chromatography reagent card shell is used to assemble and fix the test strip. The sample pad of the test strip is fixed to the sample pad fitting part of the lower cover 10. The sample application hole 21 on the upper cover 20 facing the opening side of the lower cover 10 and the guide groove 22 facing the opening side of the lower cover 10 abut against the sample pad of the test strip.

[0111] Optionally, refer to Figure 1 and Figure 9 It is understood that the width of the sample application hole 21 facing the opening side of the lower cover 10 and the width of the flow guide groove 22 facing the opening side can be smaller than the width of the test strip sample pad or the width of the sample pad adhesive portion 11. Optionally, the width of the flow guide groove 22 facing the opening side can be equal to the width of the sample application hole 21 facing the opening side of the lower cover 10. Optionally, the depth of the flow guide groove 22 can be 0.1mm-0.5mm.

[0112] Optionally, the sample pad of the test strip can be 10mm-14mm in length and 3.4mm-3.6mm in width, such as 3.5mm; (Reference) Figure 4 It is understood that the length of the sample feeding hole 21 away from the opening side of the lower cover 10 can be 8mm-12mm and the width can be 8mm-12mm; the length of the sample feeding hole 21 facing the opening side of the lower cover 10 can be 5mm-7.5mm and the width can be 2mm-3.5mm.

[0113] Optionally, refer to Figure 4 It is understood that the information bar 40 can be located on the side of the upper cover 20 away from the lower cover 10, and the information on the information bar 40 can be set in a concave shape. The depth of the concavity of the information on the information bar 40 on the upper cover 20 can be 0.1mm-0.3mm.

[0114] Optionally, the distance from the center of the sample application hole 21 to the center of the detection window 23 along the length of the top cover 20 can be 20mm-28mm.

[0115] Optionally, refer to Figure 1 and Figure 10 The test strip may include a sample pad, a marking pad, a buffer pad, a nitrocellulose membrane, and an absorbent pad arranged sequentially along its length. The upper cover 20 has a first pressure rib 24a and a second pressure rib 24b protruding towards the lower cover 10. The first pressure rib 24a abuts against the overlapping area of ​​the test strip marking pad and the buffer pad, achieving contact and pressure with the overlapping area; the second pressure rib 24b abuts against the overlapping area of ​​the test strip buffer pad and the nitrocellulose membrane, achieving contact and pressure with the overlapping area. The specific type of absorbent pad is not particularly limited, and those skilled in the art can flexibly select it according to actual needs. For example, the absorbent pad may include, but is not limited to, absorbent paper.

[0116] Optionally, refer to Figure 1 , Figure 2 or Figure 3 Understandably, the upper cover 20 includes a first pressing pin 25 and a second pressing pin 26 protruding toward the lower cover 10. The first pressing pin 25 abuts against the sample pad of the test strip, and the second pressing pin 26 abuts against the absorbent pad of the test strip, causing the test strip to adhere tightly to the lower cover and be flatly fixed to the lower cover of the housing.

[0117] Optionally, refer to Figure 9 or Figure 10 It is understood that the lower cover 10 may include at least one pair of first limiting pins 16 and at least one pair of second limiting pins 17. Each pair of first limiting pins 16 is disposed on both sides of the test strip along its width direction and abuts against both sides of the test strip sample pad along its width direction. Each pair of second limiting pins 17 is disposed on both sides of the test strip along its width direction and abuts against both sides of the test strip absorbent pad along its width direction.

[0118] It should be noted that the features and effects described for the chromatography reagent card shell of the first aspect of this application also apply to the chromatography reagent card of the second aspect of this application, and will not be repeated here.

[0119] The experimental examples described below are exemplary and are used only to explain this application, and should not be construed as limiting this application. Where specific techniques or conditions are not specified in the experimental examples, they should be performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0120] Experimental Example 1

[0121] A chromatography reagent card includes a test strip and a chromatography reagent card casing.

[0122] The test strip is assembled and fixed inside the chromatography reagent cartridge. The test strip includes a sample pad, a marking pad, a buffer pad, a nitrocellulose membrane, and an absorbent pad arranged sequentially along its length. The sample pad is 10 mm long and 3.5 mm wide.

[0123] The chromatography reagent cartridge includes a sealed upper cover and a lower cover, with an opening groove between the upper and lower covers. Specifically:

[0124] The lower cover includes: a sample pad bonding portion, a marking pad bonding portion, a buffer pad bonding portion, a nitrocellulose membrane bonding portion, and an absorbent pad bonding portion, which are distributed sequentially along its length, with an overlapping area between adjacent bonding portions; as well as a second reinforcing rib (protruding towards the upper cover), a pair of first limiting pins, and three pairs of second limiting pins. The pair of first limiting pins is located on both sides of the sample pad bonding portion along the width direction of the lower cover and abuts against both sides of the sample pad of the test strip in the width direction. The three pairs of second limiting pins are spaced apart along the length direction of the lower cover, and each pair of second limiting pins is located on both sides of the absorbent pad bonding portion along the width direction of the lower cover and abuts against both sides of the absorbent pad of the test strip in the width direction.

[0125] The top cover includes: a sample inlet, a flow guide, a detection window, a first reinforcing rib, a first pressure rib, a second pressure rib, a first pressure pin, a second pressure pin, and an information panel. Among these:

[0126] The sample feeding hole extends through the thickness direction of the upper cover and has a polished surface. The outer diameter of the sample feeding hole is larger on the side away from the opening of the lower cover, with a length of 10 mm and a width of 10 mm. The length of the sample feeding hole on the side facing the opening of the lower cover is 5 mm and the width is 2 mm.

[0127] The flow guide groove extends along the length of the upper cover and connects with the side of the sample dispensing hole facing the lower cover. Flow guide grooves are provided on both sides of the sample dispensing hole along the length of the upper cover. The depth of the flow guide groove is 0.4 mm. The width of the flow guide groove is the same as the width of the opening side of the sample dispensing hole facing the lower cover. The orthographic projection of the opening side of the sample dispensing hole and the flow guide groove facing the lower cover on the lower cover is located in the area where the sample pad is attached, and it abuts against the sample pad of the test strip.

[0128] The detection window extends through the thickness direction of the upper cover, and its orthographic projection onto the lower cover's opening side is located within the area where the nitrocellulose membrane is attached; along the length of the upper cover, the distance from the center of the sample application hole to the center of the detection window is 24 mm.

[0129] The first reinforcing rib is positioned to protrude towards the lower cover.

[0130] The first and second pressure bones are protruding towards the lower cover. The orthographic projection of the first pressure bone on the lower cover is located in the overlapping area of ​​the marking pad and the buffer pad, and abuts against the overlapping area on the test strip. The orthographic projection of the second pressure bone on the lower cover is located in the overlapping area of ​​the buffer pad and the nitrocellulose membrane, and abuts against the overlapping area on the test strip.

[0131] The first and second pressure pins are protruding towards the lower cover. The orthographic projection of the first pressure pin on the lower cover is located in the area where the sample pad is attached and abuts against the sample pad of the test strip. The orthographic projection of the second pressure pin on the lower cover is located in the area where the absorbent pad is attached and abuts against the absorbent pad of the test strip.

[0132] The information bar is located on the surface of the upper cover opposite to the lower cover. The information on the information bar is concave and has a depth of 0.2mm. The information on the information bar includes logo information.

[0133] Example 2-11

[0134] The differences between Examples 2-11 and Example 1 are detailed in Table 1.

[0135] Examples 12-15

[0136] The differences between Examples 12-15 and Example 3 are detailed in Table 2.

[0137] Test section:

[0138] Compare the loading and leakage of whole blood samples with different sample well lengths and widths and different flow channel depths.

[0139] (1) The non-sample rejection rate and leakage rate of Examples 1-11 were tested using whole blood samples. The test results are shown in Table 1. Among them:

[0140] "No sample collection" refers to the phenomenon where, after adding a whole blood sample, the liquid does not completely spread across the nitrocellulose membrane and fail to reach the absorbent paper within 8 minutes during the chromatography process from the sample pad to the label pad, then to the buffer pad, the nitrocellulose membrane, and finally the absorbent pad. The no-sample collection rate refers to the percentage of reagent cards exhibiting this phenomenon out of all reagent cards used in the experiment. No sample collection will lead to inaccurate test results.

[0141] Leakage refers to the phenomenon where, 8 minutes after adding a whole blood sample, the liquid flows from the test strip onto the lower cover or into the gap between the test strip and the lower cover when the top and bottom covers of the reagent card are opened. The leakage rate refers to the percentage of reagent cards exhibiting this phenomenon out of all reagent cards used in the experiment. Leakage will lead to inaccurate test results.

[0142] As can be seen from Examples 1-11, when the sample pad is 10mm long and 3.5mm wide, the length and width of the side of the sample application hole facing the lower cover are both narrower than the length and width of the sample pad (adhesive portion). This ensures normal whole blood sample loading without leakage during chromatography. Specifically, a length of 5.5mm and a width of 2.5mm for the side of the sample application hole facing the lower cover results in optimal whole blood sample loading and minimizes leakage. Wider or longer openings are more prone to leakage, while narrower openings are more likely to result in no sample loading. Therefore, a length of 5.5mm and a width of 2.5mm for the side of the sample application hole facing the lower cover is preferred.

[0143] Table 1. Differences between Examples 1-11 and related test results

[0144]

[0145] (2) Whole blood samples were used to test the non-sample rate, leakage rate, and red blood cell coating rate in Examples 3, 12-15. The test results are shown in Table 2. Among them:

[0146] Red blood cell adhesion refers to the phenomenon where, 8 minutes after the addition of a whole blood sample, red blood cells are observed to have migrated onto a nitrocellulose membrane through the detection window. The red blood cell adhesion rate refers to the percentage of reagent cards exhibiting this phenomenon out of all reagent cards used in the experiment. Red blood cell adhesion can lead to inaccurate testing.

[0147] Table 2. Differences between Examples 12-15 and Example 3, and related test results.

[0148] project Depth of the guide channel (mm) Non-sample rate Leakage rate Red blood cell membrane coverage Example 12 0.1 1.8％ 30.8％ 0.0％ Example 13 0.2 1.5％ 25.6％ 0.0％ Example 14 0.3 0.0％ 7.2％ 0.0％ Example 3 0.4 0.0％ 0.0％ 0.0％ Example 15 0.5 1.5％ 0.0％ 5.4％

[0149] As can be seen from Examples 3 and 12-15, when the depth of the flow channel is 0.4 mm, whole blood samples are loaded normally, there is no leakage during chromatography, and red blood cells do not adhere to the membrane. Therefore, a flow channel depth of 0.4 mm is preferred.

[0150] The present application has been described in detail above with general descriptions and specific embodiments. However, modifications or improvements can be made to it, and it can be combined arbitrarily as needed, which is obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present application fall within the scope of protection claimed in this application.

Claims

1. A chromatography reagent cartridge, characterized in that, include: The lower cover includes a sample pad fitting portion; and An upper cover and a lower cover are detachably fitted together, and at least one of the upper cover and the lower cover is provided with a reinforcing rib. The upper cover includes: A sample dispensing hole that extends through the thickness direction of the upper cover; and The flow guide channel is connected to the side of the sample dispensing hole facing the lower cover. The orthographic projection of the opening side of the sample dispensing hole facing the lower cover and the opening side of the flow guide channel facing the lower cover on the lower cover is located in the area where the sample pad is attached.

2. The chromatography reagent cartridge according to claim 1, characterized in that, The flow channel extends along the length of the upper cover; and / or, The width of the sample dispensing hole facing the opening of the lower cover and the width of the guide groove facing the opening are smaller than the width of the sample pad fitting portion; and / or, The depth of the guide channel is 0.1mm-0.5mm; and / or, The reinforcing rib includes a first reinforcing rib and / or a second reinforcing rib, wherein the first reinforcing rib is disposed on the side of the upper cover facing the lower cover, and the second reinforcing rib is disposed on the side of the lower cover facing the upper cover; and / or, The surface of the sample loading hole is polished; and / or, The surface roughness Ra of the sample loading orifice is 0.02 μm-0.1 μm; and / or, The area of ​​the sample dispensing hole facing the opening of the lower cover is smaller than the area of ​​the sample dispensing hole facing away from the opening of the lower cover; and / or, The upper cover has an information panel on the side opposite to the lower cover, and the information on the information panel is raised and recessed on the surface of the upper cover; and / or, The lower cover has an information panel on the side opposite to the upper cover, and the information on the information panel is raised and recessed on the surface of the lower cover; and / or, The lower cover includes a nitrocellulose membrane bonding portion; the upper cover includes a detection window extending through the thickness direction of the upper cover, and the orthographic projection of the opening side of the detection window facing the lower cover onto the lower cover is located within the area where the nitrocellulose membrane bonding portion is situated; and / or, The upper cover also includes a pressure bone, which protrudes toward the lower cover and extends along the width direction of the upper cover; the lower cover includes a sample pad bonding portion, a marking pad bonding portion, a nitrocellulose membrane bonding portion and an absorbent pad bonding portion distributed sequentially along its length direction, with an overlapping area between two adjacent bonding portions, and the orthographic projection of the pressure bone on the lower cover is located within the overlapping area. And / or, The upper cover also includes a first pressure pin, which protrudes toward the lower cover, and the orthographic projection of the first pressure pin on the lower cover is located in the area where the sample pad is attached. And / or, The upper cover also includes a second pressure pin, which protrudes toward the lower cover; the lower cover also includes an absorbent pad fitting portion, and the orthographic projection of the second pressure pin on the lower cover is located in the area where the absorbent pad fitting portion is located; And / or, The lower cover includes at least one pair of first limiting pins, each pair of first limiting pins being disposed on both sides of the sample pad fitting portion along the width direction of the lower cover and defining the width of the sample pad fitting portion; and / or, The lower cover includes an absorbent pad fitting portion and at least one pair of second limiting pins, each pair of second limiting pins being disposed on both sides of the absorbent pad fitting portion along the width direction of the lower cover and defining the width of the absorbent pad fitting portion; and / or, A cover opening groove is provided between the upper cover and the lower cover; and / or, The upper cover has a first connecting portion on the side facing the lower cover, and the lower cover has a second connecting portion on the side facing the upper cover. The second connecting portion is engaged with the first connecting portion.

3. The chromatography reagent cartridge according to claim 2, characterized in that, The guide channels are symmetrically arranged along the sample feeding holes; and / or, The width of the opening side of the guide channel is equal to the width of the sample dispensing hole facing the opening side of the lower cover.

4. The chromatography reagent cartridge according to claim 2 or 3, characterized in that, The reinforcing rib includes a first reinforcing rib and / or a second reinforcing rib, wherein the first reinforcing rib includes a first sub-reinforcing rib extending along the length direction of the upper cover and a second sub-reinforcing rib extending along the width direction of the upper cover; and / or The second reinforcing rib includes a third sub-reinforcing rib extending along the length direction of the lower cover and a fourth sub-reinforcing rib extending along the width direction of the lower cover.

5. The chromatography reagent cartridge according to claim 4, characterized in that, The upper cover includes at least two first sub-reinforcing ribs spaced apart along its width, each first sub-reinforcing rib being connected to a plurality of second sub-reinforcing ribs spaced apart along the length of the upper cover, and at least a portion of the second sub-reinforcing ribs being connected to the first reinforcing ribs and extending to the edge of the upper cover; and / or, The lower cover includes at least two third sub-reinforcing ribs spaced apart along its width direction, each of the third sub-reinforcing ribs being connected to a plurality of fourth sub-reinforcing ribs spaced apart along the length direction of the lower cover, and at least a portion of the fourth sub-reinforcing ribs being connected to the third sub-reinforcing ribs and extending to the edge of the lower cover.

6. The chromatography reagent cartridge according to claim 5, characterized in that, The lower cover includes at least two third sub-reinforcing ribs, and the sample pad fitting portion is located between two adjacent third sub-reinforcing ribs.

7. The chromatography reagent cartridge according to claim 2 or 6, characterized in that, The sample pad fitting portion has a length of 10mm-14mm and a width of 3.4mm-3.6mm; and / or, The length and width of the sample dispensing hole opposite to the opening of the lower cover are 8mm-12mm; and / or, The length of the sample dispensing hole facing the opening of the lower cover is 5mm-7.5mm, and the width is 2mm-3.5mm; and / or, The top cover includes a detection window, and the distance from the center of the sample application hole to the center of the detection window is 20mm-28mm along the length of the top cover.

8. The chromatography reagent cartridge according to claim 2 or 6, characterized in that, The upper cover and / or the lower cover are provided with information panels; The information in the information bar is arranged in a concave shape; and / or, The information panel is located on the side of the upper cover opposite to the lower cover, and the height difference between the information on the information panel and the surface of the upper cover is 0.1mm-0.3mm; and / or, The information panel is located on the side of the lower cover opposite to the upper cover, and the height difference between the information on the information panel and the surface of the lower cover is 0.1mm-0.3mm; and / or, The information displayed in the information bar includes one or more of the following: text, symbols, graphic icons, QR codes, and barcodes.

9. The chromatography reagent cartridge according to claim 2 or 6, characterized in that, The upper cover further includes a pressure bone, and the lower cover includes the sample pad bonding portion, the marking pad bonding portion, the nitrocellulose membrane bonding portion, and the absorbent pad bonding portion, which are sequentially distributed along its length. The lower cover also includes a buffer pad bonding portion, which is located between the marking pad bonding portion and the nitrocellulose membrane bonding portion. The pressure bone includes a first pressure bone and / or a second pressure bone. The orthographic projection of the first pressure bone on the lower cover is located in the overlapping area of ​​the marking pad bonding portion and the buffer pad bonding portion, and the orthographic projection of the second pressure bone on the lower cover is located in the overlapping area of ​​the buffer pad bonding portion and the nitrocellulose membrane bonding portion. And / or, The upper cover further includes a first pressing stud, the first pressing stud comprising at least two sub-pressing studs spaced apart along the width direction of the upper cover; and / or The upper cover has multiple rows of second pressure pins on the side facing the lower cover. These rows of second pressure pins are spaced apart along the length of the upper cover, and each row includes at least two sub-pressure pins spaced apart along the width of the upper cover; and / or, The lower cover includes an absorbent pad fitting portion and multiple pairs of second limiting pins spaced apart along the length direction of the lower cover.

10. A chromatography reagent card, characterized in that, The device includes a test strip and a chromatographic reagent cartridge according to any one of claims 1-9, wherein the test strip is sandwiched between an upper cover and a lower cover.

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