Biological reaction device

Abstract

The utility model discloses a biological reaction device, relates to bioreactor technical field, including container, executive device, sensor and control device, executive device includes base and be equipped with a plurality of stirring paddle on the base, the base detachable installation is in the container, the stirring paddle can be inserted in the container, the stirring paddle includes rotating shaft and a plurality of paddle blade, the blades are inclined to the horizontal plane, the rotation directions of every two adjacent stirring paddles are opposite, liquid in the containing cavity can flow in the direction of the rotation axis of the stirring paddles in the rotation process of the stirring paddles, and the mixing effect of cells and a culture solution is improved. The sensor is inserted into the channel of the rotating shaft and extends out of the stirring paddle, parameters in a culture solution can be detected in real time, and the control device can receive a detection signal of the sensor and control the rotating speed of the stirring paddle based on the detection signal, so that full contact and reaction of cells and the culture solution are guaranteed, and the biological culture effect is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological reactor technical field, especially a kind of biological reaction device. BACKGROUND

[0002] Biological reaction device, i.e. bioreactor, is a highly engineered equipment, used to promote the growth, reproduction or metabolism of biological active substances (such as microorganisms, plant cells, animal cells or enzymes) under controlled conditions, to produce specific biological products or carry out biological transformation process. The current biological reaction device is provided with a device for stirring, which improves the mixing degree of cells and culture solution by stirring to achieve the effect of improving reaction rate. However, its stirring function is single, and the stirring effect is not good. SUMMARY

[0003] The utility model aims at at least one of the technical problems existing in prior art. Therefore, the utility model provides a kind of biological reaction device, can improve stirring effect, improve biological reaction rate.

[0004] According to the biological reaction device of the utility model embodiment, comprising:

[0005] Container, is equipped with container cavity;

[0006] Execution device, including base and the several stirring paddles of being arranged on base, base is detachably installed on container, stirring paddle can be inserted in container cavity, the rotation direction of adjacent two stirring paddles is opposite, and stirring paddle includes shaft and the several paddle blades that are arranged along the circumference of shaft, paddle blade is inclined to horizontal plane, and shaft has the passage that penetrates both ends;

[0007] Sensor, is inserted in passage and extends outside stirring paddle, and sensor is used to detect solution in container cavity and generates detection signal;

[0008] Control device, with execution device and sensor electric connection, and control device is equipped with switch, and control device can receive detection signal from sensor, and can control the rotation speed of stirring paddle based on detection signal.

[0009] The biological reaction device has the following beneficial effects: the container, the executing device, the sensor and the control device are provided, the container is provided with a cavity, the executing device comprises a base and a plurality of stirring paddles arranged on the base, the base is detachably mounted on the container, the stirring paddles are inserted into the cavity, the stirring paddles comprise a rotating shaft and a plurality of blades arranged along the circumference of the rotating shaft, the blades are inclined to the horizontal plane, the rotating shaft has a channel penetrating through both ends, the channel helps the liquid in the cavity to flow along the rotating axis of the stirring paddles during the rotation of the stirring paddles, improves the mixing effect of the cells and the culture solution, and helps to promote the reaction rate. In addition, the rotating directions of the adjacent two stirring paddles are opposite, which helps to improve the stirring efficiency of the liquid in the cavity and the mixing efficiency of the cells and the culture solution, thereby improving the reaction rate. The sensor is inserted into the channel and extends out of the stirring paddles, which is beneficial to improve the space compactness while being capable of detecting the parameters in the culture solution in real time. The sensor is used for detecting the solution in the cavity and generating a detection signal, the control device is capable of receiving the detection signal from the sensor, and the control device is capable of controlling the rotating speed of the stirring paddles based on the detection signal, so as to automatically adjust the rotating speed of the stirring paddles according to the state parameters of the culture solution in real time, so as to ensure that the cells and the culture solution are fully contacted and reacted, and the biological culture effect is improved.

[0010] According to the embodiment of the utility model, the one end of rotating shaft away from stirring paddle is equipped with drive gear, the drive gear on the rotating shaft of adjacent two mutually engages.

[0011] According to the embodiment of the utility model, the executing device is equipped with motor, the motor is installed on base and is connected with any one stirring paddle power.

[0012] According to the embodiment of the utility model, drive gear includes driving wheel and driven wheel, and the driving wheel is arranged on the stirring paddle connected with the motor, and the driving wheel is engaged with the driven wheel.

[0013] According to the embodiment of the utility model, the stirring paddle includes the first stirring paddle and the second stirring paddle arranged adjacently, and the inclination direction of the blades between the first stirring paddle and the second stirring paddle is opposite.

[0014] According to the embodiment of the utility model, the base is provided with a positioning edge, and the outer edge of the container is provided with a limiting groove, and the positioning edge is inserted into the limiting groove when the executing device is installed on the container.

[0015] According to the embodiment of the utility model, a plurality of unit cavities are arranged in the cavity, and one stirring paddle is inserted into each unit cavity.

[0016] According to the embodiment of the utility model, the blade includes a connecting end close to the rotating shaft and a free end away from the rotating shaft, the inclination angle between the outer edge of the free end and the axis of the rotating shaft is a first angle, the inclination angle between the edge of the connecting end and the axis of the rotating shaft is a second angle, and the first angle is not equal to the second angle.

[0017] According to the embodiment of the present application, the base is provided with a covering upper cover, and the motor is mounted on the upper cover and connected with the stirring paddle.

[0018] According to the embodiment of the present application, the upper cover is provided with a mounting groove, and one end of the sensor away from the stirring paddle is fixed in the mounting groove.

[0019] The additional aspects and advantages of the present application will be partially given in the following description, and will become apparent from the following description, or will be understood by those skilled in the art through the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0020] The present application will be further described below in combination with the drawings and embodiments, wherein:

[0021] Figure 1 It is a shaft view of the biological reaction device in the embodiment of the present application;

[0022] Figure 2 It is a bottom view of the execution device in the embodiment of the present application;

[0023] Figure 3 It is a shaft view of the execution device in the embodiment of the present application;

[0024] Figure 4 It is a shaft view of the first container in the embodiment of the present application;

[0025] Figure 5 It is a shaft view of the second container in the embodiment of the present application;

[0026] Figure 6 It is a first sectional view of the execution device in the embodiment of the present application;

[0027] Figure 7 It is Figure 6 the enlarged view of A in the figure;

[0028] Figure 8 It is a second sectional view of the execution device in the embodiment of the present application;

[0029] Figure 9 It is Figure 8 the enlarged view of B in the figure.

[0030] REFERENCE NUMERALS:

[0031] Execution device 100; base 101; upper cover 102; motor 103; mounting groove 104; driving wheel 105; driven wheel 106; switch 107; positioning edge 108; limiting groove 109;

[0032] First stirring paddle 110; paddle 111; shaft 112; passage 113; first axis 114; connecting end 115; free end 116; first angle 117; second angle 118; second axis 119;

[0033] Second stirring paddle 120; cavity 121; unit cavity 122; sensor 123; container 124. DETAILED DESCRIPTION

[0034] The embodiments of the present application are described in detail below, examples of which are shown in the drawings, wherein the same or similar notations represent the same or similar elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only, and are used only for explaining the present application, and cannot be understood as a limitation of the present application.

[0035] In the description of the present application, it is understood that the orientation description, such as the orientation or position relationship indicated by up, down, front, back, left, right, etc. is based on the orientation or position relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as a limitation of the present application.

[0036] In the description of the present application, the meaning of several is one or more, and the meaning of multiple is two or more, greater than, less than, more than, etc. are understood as not including the number, and above, below, etc. are understood as including the number. If it is described as first, second, it is only used for distinguishing technical features for the purpose, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the sequence of indicated technical features.

[0037] In the description of the present application, unless otherwise explicitly limited, the words such as setting, installing, connecting, etc. should be broadly understood, and the person skilled in the art can reasonably determine the specific meaning of the above words in the present application according to the specific content of the technical solution.

[0038] Referring to Figure 1 and Figure 2 In one biological reaction device of the present application, the container 124 is provided with a cavity 121, and the execution device 100 includes a base 101 and a plurality of stirring paddles arranged on the base 101. The base 101 is detachably mounted on the container 124, and it can be understood that the execution device 100 includes a plurality of stirring paddles arranged on the base 101. Figure 3 and Figure 4The base 101 is provided with a positioning edge 108 extending to the bottom direction, and the outer edge of the container 124 is provided with a limiting groove 109, when the executing device 100 is installed on the container 124, the positioning edge 108 can be inserted into the limiting groove 109, so as to realize the installation and positioning of the executing device 100 and the container 124, and improve the use convenience.

[0039] It can be understood that six stirring paddles are arranged in the embodiment, the stirring paddles can be inserted into the accommodating cavity 121, and the stirring paddles are used for stirring the culture solution in the accommodating cavity 121 so as to make the culture solution and the cells fully contact. The stirring paddle comprises a rotating shaft 112 and a plurality of paddle blades 111 arranged along the circumferential direction of the rotating shaft 112, and the paddle blade 111 is inclined to the horizontal plane, which is helpful to make the liquid in the accommodating cavity 121 flow along the direction of the rotating axis of the stirring paddle during the rotation of the stirring paddle, improve the mixing effect of the cells and the culture solution, and help to promote the reaction rate. Meanwhile, the executing device 100 is provided with a motor 103, the base 101 is provided with a covering upper cover 102, the motor 103 is installed on the upper cover 102 and is power-connected with any one of the stirring paddles, the end of the rotating shaft 112 away from the stirring paddle is provided with a driving gear, the driving gears on the adjacent two rotating shafts 112 are meshed with each other, so that the rotating directions of the adjacent two stirring paddles are opposite, which is helpful to improve the stirring efficiency of the liquid in the accommodating cavity 121, improve the mixing efficiency of the cells and the culture solution, and thus improve the reaction rate. Further, the driving gear comprises a driving wheel 105 and a driven wheel 106, the stirring paddle power-connected with the motor 103 is provided with the driving wheel 105, the driving wheel 105 is meshed with the driven wheel 106, so as to realize the power transmission.

[0040] Referring to 2 and Figure 3, the stirring paddle comprises a first stirring paddle 110 and a second stirring paddle 120 arranged adjacently, and the tilting directions of the paddle blades 111 between the first stirring paddle 110 and the second stirring paddle 120 are opposite. It can be understood that during the cell culture process, cells are deposited at the bottom of the cavity 121, and the cells consume the dissolved oxygen and nutrients of the culture solution in the process of continuous growth and metabolism, thereby causing the concentration of nutrients and the pH value at the bottom layer to be low, which is not conducive to the continuous growth of cells. Therefore, when the motor 103 drives the driving wheel 105 to rotate, the driving wheel 105 is externally meshed with the driven wheel 106, so that the rotating directions of the driving wheel 105 and the driven wheel are opposite, the stirring paddle installed on the driving wheel 105 and the stirring paddle installed on the driven wheel rotate in opposite directions, and when rotating, different direction eddies can be generated in the cavity 121, which can improve the mixing effect of the culture solution and the cells and improve the utilization rate of nutrients by the cells, thereby facilitating the growth of the cells. At the same time, since the tilting directions of the paddle blades 111 between the first stirring paddle 110 and the second stirring paddle 120 arranged adjacently are opposite, that is, the first stirring paddle 110 and the second stirring paddle 120 are mirror images of each other, that is, the tilting directions of the paddle blades 111 between the stirring paddle installed on the driving wheel 105 and the stirring paddle installed on the driven wheel 106 are opposite, and the rotating directions of the first stirring paddle 110 and the second stirring paddle 120 are opposite, therefore, when rotating, the first stirring paddle 110 and the second stirring paddle 120 can make the culture solution move in the same direction along the rotating axis in the culture solution, thereby causing convection between the upper layer and the lower layer of the culture solution, and the cells at the bottom of the cavity 121 float upward, thereby facilitating the uniform distribution of nutrients and dissolved oxygen in the culture solution and being conducive to the growth of cells.

[0041] Further, referring to Figure 2 , the paddle blade 111 comprises a connecting end 115 close to the rotating shaft 112 and a free end 116 away from the rotating shaft 112, the tilting angle of the outer edge of the free end 116 to the axis of the rotating shaft 112 is a first angle 117, the tilting angle of the edge of the connecting end 115 to the axis of the rotating shaft 112 is a second angle 118, and the first angle 117 and the second angle 118 are not equal. Referring to Figure 6 and Figure 7The first axis 114 is defined as a straight line passing through the paddle 111 and parallel to the outer edge of the free end 116 along the width direction of the paddle 111, the second axis 119 is defined as a straight line passing through the paddle 111 and parallel to the edge of the connecting end 115, the first angle 117 is the included angle between the first axis 114 and the rotation axis, and the second angle 118 is the included angle between the second axis 119 and the rotation axis. In the embodiment, the first angle 117 is greater than the second angle 118, that is, the inclination angle of the free end 116 relative to the rotation axis is greater than the inclination angle of the connecting end 115 relative to the rotation axis. In the embodiment, at least three paddles 111 are provided. Since the connecting end 115 of the paddle 111 is closer to the rotation axis, the circumferential width of the paddle 111 is larger when the inclination angle is too large, which is not conducive to the spatial arrangement of the plurality of paddles 111. The free end 116 is located at the outer periphery of the stirring paddle and has sufficient arrangement space. Therefore, when the first angle 117 is greater than the second angle 118, the surface of the paddle 111 can have a curved surface, and the stirring paddle can effectively reduce the resistance and improve the stirring efficiency of the liquid when rotating.

[0042] Referring to Figure 5 The container cavity 121 can also be provided with a plurality of unit cavities 122, and each unit cavity 122 is inserted with a stirring paddle. Different types of microorganisms can be placed in each unit cavity 122 for cultivation. Therefore, the execution device 100 can simultaneously stir different culture dishes, thereby improving the use flexibility. Further, a plurality of motors 103 can be provided, and each stirring paddle is driven by a separate motor 103. The stirring speed can be adjusted according to different culture dishes to meet the use requirements.

[0043] Referring to Figure 8 and Figure 9 The shaft 112 has a channel 113 passing through both ends. The execution device 100 is provided with a sensor 123 for detecting the pH value and dissolved oxygen concentration in the culture solution. The sensor 123 is installed on the base 101 and inserted into the channel 113 to extend outside the stirring paddle. The radial outer periphery wall of the sensor 123 and the inner wall of the channel 113 have a gap therebetween to avoid the sensor 123 from rotating with the stirring paddle. The sensor 123 can detect the solution in the container cavity 121 and generate a detection signal. The control device can receive the detection signal from the sensor 123, and the control device can control the rotation speed of the stirring paddle based on the detection signal. The rotation speed of the stirring paddle can be automatically adjusted in real time according to the state parameters of the culture solution to ensure that the cells and the culture solution are in sufficient contact and reaction, thereby improving the biological cultivation effect. Further, the upper cover 102 is provided with a mounting groove 104, and the end of the sensor 123 away from the stirring paddle is fixed in the mounting groove 104.

[0044] It can be understood that the control device further comprises a switch 107, which is pivotally connected to the top of the upper cover, and is used to realize the opening and closing of the execution device 100.

[0045] The utility model embodiment has been explained in detail above in combination with the drawings, but the utility model is not limited to the above-mentioned embodiment, and various changes can be made within the knowledge range possessed by the ordinary skilled in the art without departing from the purpose of the utility model.

Claims

1. A bioreactor, characterized in that, include: The container has a cavity; An actuator includes a base and a plurality of stirring paddles disposed on the base. The base is detachably mounted on the container. The stirring paddles can be inserted into the cavity. The rotation directions of two adjacent stirring paddles are opposite. Each stirring paddle includes a rotating shaft and a plurality of blades arranged circumferentially along the rotating shaft. The blades are inclined to a horizontal plane. The rotating shaft has a channel passing through both ends. A sensor, inserted into the channel and extending out of the stirring paddle, is used to detect parameters of the solution in the cavity and generate a detection signal; A control device is electrically connected to the actuator and the sensor. The control device is equipped with a switch. The control device is able to receive the detection signal from the sensor and control the rotation speed of the stirring paddle based on the detection signal.

2. The bioreactor according to claim 1, characterized in that, A drive gear is provided at one end of the rotating shaft away from the stirring paddle, and the drive gears on two adjacent rotating shafts mesh with each other.

3. A bioreactor according to claim 2, characterized in that, The actuator is equipped with a motor, which is mounted on the base and powered by any one of the stirring paddles.

4. A bioreactor according to claim 3, characterized in that, The drive gear includes a driving gear and a driven gear. The stirring paddle, which is powered by the motor, is equipped with a driving gear, and the driving gear meshes with the driven gear.

5. A bioreactor according to claim 1, characterized in that, The agitator includes a first agitator and a second agitator arranged adjacent to each other, with the blades of the first agitator and the second agitator having opposite inclination directions.

6. A bioreactor according to claim 1, characterized in that, The base is provided with a positioning edge, and the outer edge of the container is provided with a limiting groove. When the actuator is installed on the container, the positioning edge is inserted into the limiting groove.

7. A bioreactor according to claim 1, characterized in that, The cavity is provided with several unit cavities, and each unit cavity is fitted with a stirring paddle.

8. A bioreactor according to claim 7, characterized in that, The blade includes a connecting end near the rotating shaft and a free end away from the rotating shaft. The outer edge of the free end is inclined at a first angle to the axis of the rotating shaft, and the edge of the connecting end is inclined at a second angle to the axis of the rotating shaft. The first angle and the second angle are not equal.

9. A bioreactor according to claim 3, characterized in that, The base is provided with a cover, and the motor is mounted on the cover and is powered by any one of the stirring paddles.

10. A bioreactor according to claim 9, characterized in that, The upper cover has a mounting groove, and the end of the sensor away from the stirring paddle is fixed in the mounting groove.

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