Purification equipment for preparing nicotinamide adenine dinucleotide phosphate

Abstract

The utility model relates to the related technical field of preparation of nicotinamide adenine dinucleotide phosphate, and discloses purification equipment for preparation of nicotinamide adenine dinucleotide phosphate, which comprises a main body component, the main body component comprises an external tank body, a funnel is arranged at the center of an inner cavity of the external tank body, and a numerical control valve is arranged at a material leakage port of the funnel. And a filter screen is arranged below the funnel. When the device is used, a water source can push the outlet valve plate to move when entering the one-way valve, then the first adjusting part presses the second adjusting part, the waterproof baffle rotates, finally, the water outlet hole is communicated with the interior of the externally-mounted tank body, the center shaft is driven by the power element to rotate and flush all the time, and the flushing strength is greatly improved; the problems that in an existing scheme, objects formed by fresh squeezed yeast and liquid in an external tank body can be bonded to the inner wall of a barrel body and a stirring piece, the washing force is poor, nicotinamide adenine dinucleotide phosphate is prone to being left, and the purity of extracted nicotinamide adenine dinucleotide phosphate is low are solved.

Description

Technical Field

[0001] This utility model relates to the technical field of nicotinamide adenine dinucleotide phosphate preparation, specifically a purification device for nicotinamide adenine dinucleotide phosphate preparation. Background Technology

[0002] Nicotinamide adenine dinucleotide phosphate (NAPDP) is a nucleotide derivative composed of nicotinamide (a form of vitamin B3), adenine, ribose, and a phosphate group. The nicotinamide moiety is the active site responsible for its key function. Its production methods are largely the same as those for NAD (vitamin Acid), primarily divided into chemical synthesis and biocatalysis. Biocatalysis includes fermentation and enzymatic catalysis, which has gradually become the mainstream approach due to its green, environmentally friendly, and pollution-free advantages compared to chemical synthesis. The process for preparing NAPDP from yeast generally requires cell wall disruption, extraction, and separation of the raw material, followed by the preparation of the extract for further processing. Currently, cell wall disruption and extraction / separation are performed separately, requiring transportation, which affects work efficiency, is time-consuming and labor-intensive, and results in incomplete extraction and waste, especially during filter cake washing, where the washing effect is poor.

[0003] The invention disclosed in patent number "CN213994909U" is a "Raw Material Separation Device for Preparing High-Purity NAD". Its specific technical structure includes a cylinder with a funnel fixedly installed in the middle of its inner wall. A first feeding pipe and a second feeding pipe are fixedly connected to one side of the top of the cylinder and the bottom of the other side, respectively. Both the first and second feeding pipes are threaded with caps. A stirring assembly is installed at the top of the inner wall of the cylinder. Two heating plates are fixedly installed at the top of each side of the inner wall of the cylinder. Sealing plates are fixedly connected to both sides of the top of the funnel via two electric cylinders. A filter screen is fixedly installed in the middle of the inner wall of the funnel. An aeration assembly is installed on the funnel. A discharge pipe is fixedly connected to the middle of the bottom of the funnel. The beneficial effects of this invention are: by dividing the interior of the cylinder into two cavities through the funnel for cell wall breaking extraction and separation, the two processes of raw material cell wall breaking extraction and separation can be completed simultaneously inside the cylinder, eliminating the need for back-and-forth transportation, improving work efficiency, and saving time and labor.

[0004] However, this method also has certain drawbacks. After the stirring extraction process, the upper chamber will be unable to be cleaned because the substances formed by the freshly pressed yeast and liquid will stick to the inner wall of the cylinder. This will affect the next separation process. At the same time, it is also easy to affect the purity of nicotinamide adenine dinucleotide phosphate due to incomplete rinsing.

[0005] No effective solutions have yet been proposed to address the problems in the relevant technologies. Utility Model Content

[0006] To address the shortcomings of existing technologies, this invention provides a purification device for the preparation of nicotinamide adenine dinucleotide phosphate, which solves the problem in existing solutions where the substances formed by freshly pressed yeast and liquid inside the outer packaging tank adhere to the inner wall of the cylinder and the stirring components, and the rinsing force is insufficient, easily leaving nicotinamide adenine dinucleotide phosphate residue, resulting in low purity of the extracted nicotinamide adenine dinucleotide phosphate.

[0007] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0008] A purification device for preparing nicotinamide adenine dinucleotide phosphate includes a main component enclosing an outer tank. A funnel is located at the center of the inner cavity of the outer tank, and a CNC valve is installed at the discharge port of the funnel. A filter screen is also installed below the funnel. A feed hopper and an exhaust port are fixedly connected to the top of the outer tank, and a feeding port and a discharge port are fixedly connected to the periphery of the outer tank. A crushing and rinsing assembly is installed on the outer tank, including a central shaft rotatably connected to the center of the top end wall of the outer tank. A stirring element is fixed to the periphery of the central shaft. The peripheral side is also provided with water outlet holes. A water-blocking baffle is rotatably connected to the inner cavity of the central shaft. An elastic rod is fixed on the water-blocking baffle. The end of the elastic rod away from the water-blocking baffle is fixed to the central shaft. A connector is also rotatably connected to the top of the central shaft. A one-way valve is provided in the inner cavity of the connector. The pulverizing and rinsing assembly also includes an external water pipe connected to the inlet end of the one-way valve. The valve plate at the outlet end of the one-way valve is an outlet valve plate. An adjusting component one is rotatably connected to the outlet valve plate. An adjusting component two that matches the connector is also fixed to the top of the water-blocking baffle. A power element is also provided on the central shaft.

[0009] Preferably, the feeding port is located in the area below the funnel on the outer tank body, and valves are provided on the feeding hopper, feeding port and discharge port.

[0010] Preferably, there are several agitators and several water outlets.

[0011] Preferably, a limiting rod is movably connected to the outer periphery of the adjusting member, and the limiting rod is fixed on the central shaft.

[0012] Preferably, the power element includes a motor fixed above the outer tank body, a drive wheel fixed to the motor drive shaft, and the same drive belt is provided on the peripheral side of the drive wheel and the central shaft protruding from the peripheral side of the outer tank body.

[0013] Preferably, the outer tank is also provided with a cleaning component, which includes a flushing scraper disposed in the inner cavity of the outer tank, a connecting rod fixed on the outer periphery of the central shaft, and the end of the connecting rod away from the central shaft being fixedly connected to the flushing scraper.

[0014] Compared with the prior art, this utility model provides a purification device for the preparation of nicotinamide adenine dinucleotide phosphate, which has the following beneficial effects:

[0015] 1. This utility model, through its pulverizing and rinsing assembly, allows water to enter the inner cavity of the connector via a one-way valve during rinsing. Simultaneously, the water entering the one-way valve pushes the outlet valve plate, causing the first adjusting component to press the second adjusting component, thus rotating the water-blocking baffle. Ultimately, this connects the water outlet to the interior of the outer tank. Throughout this process, the central shaft rotates under the drive of the power component. This rotational rinsing significantly improves the rinsing force, solving the problem in existing solutions where freshly pressed yeast and liquid residues inside the outer tank adhere to the inner wall of the cylinder and the stirring components, resulting in insufficient rinsing force and the easy retention of nicotinamide adenine dinucleotide phosphate, leading to low purity extraction.

[0016] 2. The present invention features a cleaning assembly, which includes a rinsing scraper disposed within the inner cavity of the outer container. A connecting rod is fixed to the outer periphery of the central shaft, and the end of the connecting rod away from the central shaft is fixedly connected to the rinsing scraper. During rinsing, the scraper can scrape the inner wall of the outer container, thereby further improving the rinsing quality. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the structure of the pulverizing and rinsing assembly of this utility model;

[0019] Figure 3 This is a schematic diagram of the cross-sectional structure of the central axis of this utility model.

[0020] In the picture:

[0021] 1. Main components; 11. Outer tank; 12. Funnel; 121. CNC valve; 122. Filter screen; 13. Feed hopper; 14. Exhaust port; 15. Feeding port; 16. Discharge port;

[0022] 2. Crushing and rinsing assembly; 21. Central shaft; 22. Agitator; 23. Water outlet; 24. Water baffle; 25. Connecting component; 26. One-way valve; 261. External water pipe; 262. Outlet valve plate; 27. Adjusting component one; 28. Adjusting component two; 281. Flexible rod; 29. ​​Power element; 291. Motor; 292. Transmission wheel; 293. Transmission belt;

[0023] 3. Cleaning components; 31. Rinse scraper; 32. Connecting rod. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] As described in the background section, there are shortcomings in the existing technology. In order to solve the above-mentioned technical problems, this application proposes a purification device for the preparation of nicotinamide adenine dinucleotide phosphate.

[0026] Please see Figure 1 - Figure 3 A purification device for preparing nicotinamide adenine dinucleotide phosphate includes a main component 1, which encloses an outer tank 11. A funnel 12 is located at the center of the inner cavity of the outer tank 11, and a CNC valve 121 is installed at the discharge port of the funnel 12. A filter screen 122 is also installed below the funnel 12. A feed hopper 13 and an exhaust port 14 are fixedly connected to the top of the outer tank 11, and a feeding port 15 and a discharge port 16 are fixedly connected to the periphery of the outer tank 11. A crushing and rinsing assembly 2 is installed on the outer tank 11. The crushing and rinsing assembly 2 includes a central shaft 21 rotatably connected to the center of the top end wall of the outer tank 11. A stirring element 22 is fixed to the periphery of the central shaft 21, and the periphery of the central shaft 21 is also open. The assembly includes a water outlet 23, a water-blocking baffle 24 rotatably connected to the inner cavity of the central shaft 21, an elastic flexible rod 281 fixed on the water-blocking baffle 24, and the end of the elastic flexible rod 281 away from the water-blocking baffle 24 fixed to the central shaft 21. A connector 25 is also rotatably connected to the top of the central shaft 21. A one-way valve 26 is provided in the inner cavity of the connector 25. The pulverizing and rinsing assembly 2 also includes an external water pipe 261 that passes through the connector 25 and is connected to the inlet end of the one-way valve 26. The valve plate at the outlet end of the one-way valve 26 is an outlet valve plate 262. An adjusting component 1 27 is rotatably connected to the outlet valve plate 262. An adjusting component 28 that is adapted to the connector 25 is also fixed to the top of the water-blocking baffle 24. A power element 29 is also provided on the central shaft 21.

[0027] When using this device, fresh pressed yeast and water are added through the feed hopper 13. The central shaft 21 is rotated by the power element 29 to stir, break the cell walls, and extract the yeast. This is the existing solution and will not be described in detail. After the cell wall breaking and extraction are completed, the device is rinsed by the stirring and cleaning component 3. First, the device is connected to a powered water source through the external water pipe 261, allowing the water to enter the device for rinsing. The water source first enters the inner cavity of the connector 25 through the one-way valve 26, and at the same time pushes the outlet valve plate 262 of the one-way valve 26, causing the first adjusting component 27 to press the second adjusting component 28, which finally causes the water baffle 24 to rotate, so that the water outlet 23 communicates with the inside of the outer tank 11, and then rinsing is performed.

[0028] When using this device,

[0029] Furthermore, the feeding port 15 is located in the area below the funnel 12 of the outer tank 11. Valves are provided on the feed hopper 13, the feeding port 15 and the discharge port 16. The valves facilitate the opening and closing of the feeding port 15 and the discharge port 16 of the feed hopper 13.

[0030] Furthermore, there are several agitators 22 and several water outlets 23. Multiple agitators 22 can improve the efficiency of agitation and wall breaking, and multiple water outlets can better rinse the entire crushing area.

[0031] Furthermore, a limiting rod is movably connected to the outer periphery of the adjusting component 27. The limiting rod is fixed on the central shaft 21 and is used to realize the movement of the adjusting component 27, so that it can only move up and down.

[0032] Furthermore, the power element 29 includes a motor 291 fixed above the outer tank 11. The power shaft of the motor 291 is fixed with a transmission wheel 292. The peripheral side of the transmission wheel 292 and the central shaft 21 protrude from the peripheral side of the outer tank 11 and are provided with the same transmission belt 293. The power element 29 mainly uses the motor 291 to provide power to drive the central shaft 21 to rotate, thereby realizing its cell wall breaking and rinsing functions.

[0033] Furthermore, a cleaning component 3 is also provided on the outer tank 11. The cleaning component 3 includes a flushing scraper 31 disposed in the inner cavity of the outer tank 11, and a connecting rod 32 is fixed on the outer periphery of the central shaft 21. The end of the connecting rod 32 away from the central shaft 21 is fixedly connected to the flushing scraper 31.

[0034] The cleaning component 3 is mainly used to rinse the side wall of the outer tank 11, so as to scrape off the mixture on the side wall.

[0035] Working principle: When using this device, freshly pressed yeast and water are added through the feed hopper 13. The power element 29 drives the central shaft 21 to rotate for stirring, cell wall breaking, and extraction. This is the existing solution and will not be described in detail. After cell wall breaking and extraction, the device is rinsed by the stirring and cleaning component 3. First, the external water pipe 261 is connected to a powered water source. The external water source needs a certain pressure. If the pressure is not high enough, a water pump can be used to increase the pressure so that the water source can enter the device for rinsing. First, the water source enters the inner cavity of the connector 25 through the one-way valve 26. At the same time, when the water source enters through the one-way valve 26, it pushes the outlet valve plate 262 of the one-way valve 26 to move, thereby driving the first regulating component 27 to press the second regulating component 28, causing the water baffle 24 to rotate. Finally, the water outlet 23 will be connected to the outer tank 11. The internal components are interconnected. During this process, the central shaft 21 rotates continuously under the drive of the power element 29. The rotational rinsing greatly improves the rinsing force and makes it easier to rinse all angles, including the stirring element 22 used for wall breaking. This greatly removes the residues and ensures the purity of the extracted nicotinamide adenine dinucleotide phosphate. At the same time, a cleaning component 3 is also provided on the outer container 11. The cleaning component 3 includes a rinsing scraper 31 set in the inner cavity of the outer container 11. A connecting rod 32 is fixed on the outer periphery of the central shaft 21. The end of the connecting rod 32 away from the central shaft 21 is fixedly connected to the rinsing scraper 31. During rinsing, the inner wall of the outer container 11 can be scraped to further improve the rinsing quality. After the rinsing and extraction are completed, the operation continues with the existing scheme until high-purity nicotinamide adenine dinucleotide phosphate is extracted.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A purification apparatus for the preparation of nicotinamide adenine dinucleotide phosphate comprising a main assembly (1), characterized in that: The main body assembly (1) is provided with an outer tank (11), a funnel (12) is arranged in the inner cavity of the outer tank (11), a numerical control valve (121) is arranged at the material leakage port of the funnel (12), a filter screen (122) is further arranged below the funnel (12), an inlet hopper (13) and an exhaust port (14) are fixedly arranged at the top end of the outer tank (11), and a feeding port (15) and a discharging port (16) are further fixedly arranged on the side surface of the outer tank (11). The outer tank (11) is provided with a crushing and flushing assembly (2), the crushing and flushing assembly (2) comprises a central shaft (21) rotatably connected to the center of the end wall at the top end of the outer tank (11), a stirring piece (22) is fixedly arranged on the side surface of the central shaft (21), a water outlet hole (23) is formed in the side surface of the central shaft (21), a water isolation baffle (24) is rotatably arranged in the inner cavity of the central shaft (21), an elastic soft rod (281) is fixedly arranged on the water isolation baffle (24), one end of the elastic soft rod (281) away from the water isolation baffle (24) is fixedly arranged on the central shaft (21), a connecting piece (25) is rotatably arranged at the top end of the central shaft (21), a one-way valve (26) is arranged in the inner cavity of the connecting piece (25), the crushing and flushing assembly (2) further comprises an external water pipe (261) connected to the inlet end of the one-way valve (26), the valve plate at the outlet end of the one-way valve (26) is an outlet valve plate (262), an adjusting piece one (27) is rotatably arranged on the outlet valve plate (262), an adjusting piece two (28) matched with the connecting piece (25) is further fixedly arranged at the top end of the water isolation baffle (24), and a power element (29) is further arranged on the central shaft (21).

2. The purification apparatus for preparing nicotinamide adenine dinucleotide phosphate according to claim 1, characterized by: The feeding port (15) is arranged below the funnel (12) of the outer tank (11), and valves are arranged on the inlet hopper (13), the feeding port (15) and the discharging port (16).

3. The purification apparatus for preparing nicotinamide adenine dinucleotide phosphate according to claim 1, characterized by: A plurality of stirring pieces (22) and a plurality of water outlet holes (23) are arranged.

4. The purification apparatus for preparing nicotinamide adenine dinucleotide phosphate according to claim 1, characterized by: The adjusting piece one (27) is movably connected with a limiting rod, and the limiting rod is fixedly arranged on the central shaft (21).

5. The purification apparatus for preparing nicotinamide adenine dinucleotide phosphate according to claim 1, characterized by: The power element (29) comprises a motor (291) fixedly arranged above the outer tank (11), a transmission wheel (292) is fixedly arranged on the power shaft of the motor (291), and the same transmission belt (293) is arranged on the side surface of the central shaft (21) and the transmission wheel (292) protruding from the side surface of the outer tank (11).

6. The purification apparatus for preparing nicotinamide adenine dinucleotide phosphate according to claim 1, characterized by: A cleaning assembly (3) is further arranged on the outer tank (11), the cleaning assembly (3) comprises a flushing scraper (31) arranged in the inner cavity of the outer tank (11), a connecting rod (32) is fixedly arranged on the outer side surface of the central shaft (21), and one end of the connecting rod (32) away from the central shaft (21) is fixedly connected with the flushing scraper (31).

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