Placing micro-mode biological large-scale synchronization collector

Abstract

The utility model relates to the technical field of experimental auxiliary instruments, and discloses a large-scale synchronous collector for placement type micro-mode organisms, which comprises a disposable suction head (1), a centrifugal tube (4) and a negative pressure pump shell (5) which are connected in sequence through disposable hoses (2), and the disposable suction head (1), the centrifugal tube (4) and the negative pressure pump shell (5) are connected through the disposable hoses (2). A rubber plug (3) is arranged at an opening of the centrifugal tube (4), the centrifugal tube (4) is sealed through the rubber plug (3), two openings are formed in the rubber plug (3) and used for fixing the disposable hose (2), and the portion, at one opening, of the disposable hose (2) extends out of the centrifugal tube (4) and is connected with the holding part; after the holding part is placed on the negative pressure pump frame (8) to be fixed, the weight is reduced when an operator uses the negative pressure pump, so that the burden of the operator is reduced.

Description

Technical Field

[0001] This utility model relates to the field of experimental auxiliary equipment technology, specifically a collector for large-scale synchronization of placed micro-model organisms. Background Technology

[0002] *C. elegans* and *Drosophila melanogaster* are two common model organisms in the life sciences. Unlike model organisms such as mice and rats, these two organisms are tiny, so many experiments require researchers to quickly collect large numbers of samples at the same developmental or adult stage. Current technical solutions include:

[0003] 1. Using platinum wire to pick out individual nematodes is suitable for experiments requiring small sample sizes. A skilled researcher with over five years of experience can identify and pick out a maximum of 300 nematodes at a specific developmental stage over 30 minutes. Furthermore, the developmental time window for larval synchronization cannot be accurately determined, typically exceeding 6 hours. For adults, the time window is even more difficult to pinpoint; most studies requiring adult synchronization must use late-stage larvae instead of adults or employ drug treatment.

[0004] 2. The method of collecting by elution with buffer solution requires long-term starvation or synchronization pretreatment with drugs (drugs that inhibit the development of the reproductive system).

[0005] Similar to nematodes, collecting fruit fly larvae requires researchers to cultivate a large number of parent fruit flies in the early stages for egg laying, hatching, and collecting larvae at the same developmental stage one by one.

[0006] Existing technology: 1. Using platinum wire to pick samples requires high speed and quality from the experimenter, is time-consuming, and results in poor synchronization between different individuals. This method is labor-intensive, slow, time-consuming, and has poor synchronization effects. It also places high demands on the experimenter's skills and cannot achieve large-scale synchronized sample collection, thus hindering the progress of related research.

[0007] 2. Using buffer elution increases the error in controlling synchronization between individuals, and samples from different stages are easily mixed into the synchronized samples, contaminating the experimental results. More seriously, using long-term starvation or drugs (drugs that inhibit reproductive system development) for synchronization pretreatment can suppress the normal physiological function of the reproductive system, leading to significant changes in gene expression in the samples and affecting the research results and conclusions.

[0008] Neither of the above two technical solutions can achieve rapid, large-scale, and accurate synchronous collection of samples. When the sample volume is large, picking with a platinum wire by hand can easily cause user fatigue, affecting work efficiency and accuracy.

[0009] In view of this, the present invention provides a collector for large-scale synchronization of placed micro-model organisms to solve the problems existing in the prior art. Utility Model Content

[0010] This utility model, by setting up a negative pressure pump frame 8 to support the equipment, and by setting up a negative pressure pump housing 5 with an internal negative pressure pump, and using a suction head 1 for suction, greatly improves work efficiency. Furthermore, placing the negative pressure pump frame 8 on a table avoids user fatigue. Therefore, this utility model is complete.

[0011] This invention provides a collector for large-scale synchronization of placed micro-model organisms, comprising a disposable pipette tip 1, a centrifuge tube 4, and a negative pressure pump housing 5 connected in sequence. The disposable pipette tip 1, centrifuge tube 4, and negative pressure pump housing 5 are connected by a disposable hose 2. A rubber stopper 3 is provided at the opening of the centrifuge tube 4 to seal the centrifuge tube 4. The rubber stopper 3 has two openings for fixing the disposable hose 2. The disposable hose 2 at one opening extends into the centrifuge tube 4, and the disposable hose 2 at the other opening extends out of the centrifuge tube 4 and connects to the negative pressure pump housing 5. A negative pressure pump is provided inside the negative pressure pump housing 5 and is connected to the disposable hose 2 extending out of the centrifuge tube 4 via the disposable hose 2. A switch 6 is also provided on the negative pressure pump housing 5 to control the working state of the negative pressure pump.

[0012] Furthermore, a negative pressure pump frame 8 is provided at the bottom of the negative pressure pump housing 5. The negative pressure pump frame 8 is an open design that can support the negative pressure pump housing 5.

[0013] Furthermore, the negative pressure pump housing 5 is also provided with a grip 7, which is made of rubber, to prevent the negative pressure pump housing 5 from slipping from the user's hand during use.

[0014] Furthermore, the outer surface of the centrifuge tube 4 is also provided with scale lines for observing the capacity inside the centrifuge tube.

[0015] Furthermore, the end of the disposable tubing 2 inserted into the centrifuge tube 4 is close to the bottom of the centrifuge tube 4 to prevent splashing inside the centrifuge tube 4 after being sucked in.

[0016] Furthermore, the negative pressure pump is equipped with an internal power source to provide kinetic energy to the negative pressure pump.

[0017] Furthermore, this device can be operated with both hands, allowing users to hold the suction head in one hand and the vacuum pump in the other, providing more alternative options for different experimental needs.

[0018] Compared with existing technologies, the present invention provides a collector for large-scale synchronization of placed micro-model organisms, which has the following technical advantages:

[0019] This invention provides a placement-type collector for large-scale synchronization of micro-model organisms. By incorporating a negative pressure pump and connecting it with piping, it enables electrically powered collection, improving operator efficiency. After the negative pressure pump housing 5 is fixed on the negative pressure pump holder 8, the operator can more flexibly adjust the sample position under the eyepiece. The grippable design of the negative pressure pump housing 5 allows the operator to hold the pipette tip 1 in one hand and the negative pressure pump housing 5 in the other, providing more alternative solutions for different experiments. The inclusion of disposable tubing 2 and disposable pipette tips 1 enables rapid switching between different sample collections, preventing cross-contamination between samples and improving work efficiency.

[0020] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a schematic diagram showing the placement of both hands when using this utility model on an operating table.

[0023] Figure 3 This is a schematic diagram of the placement method for single-handed operation when using this utility model on the operating table.

[0024] The attached figures are labeled as follows:

[0025] 1-Disposable suction head; 2-Disposable hose; 3-Rubber stopper; 4-Centrifuge tube; 5-Negative pressure pump housing; 6-Switch; 7-Grip; 8-Negative pressure pump bracket. Detailed Implementation

[0026] To further illustrate the technical means and effects adopted by this utility model in order to achieve the intended utility model purpose, the following detailed description of the specific implementation methods, structure, features and effects of this utility model is provided in conjunction with the accompanying drawings and preferred embodiments.

[0027] An embodiment of a collector for large-scale synchronization of placed micro-model organisms

[0028] like Figure 1-3 As shown

[0029] A collector for large-scale synchronization of placed microbial model organisms includes a disposable pipette tip 1, a centrifuge tube 4, and a negative pressure pump housing 5 connected in sequence. The disposable pipette tip 1, centrifuge tube 4, and negative pressure pump housing 5 are connected by a disposable hose 2. A rubber stopper 3 is provided at the opening of the centrifuge tube 4 to seal the centrifuge tube 4. The rubber stopper 3 has two openings for fixing the disposable hose 2. The disposable hose 2 at one opening extends into the centrifuge tube 4, and the disposable hose 2 at the other opening extends out of the centrifuge tube 4 and connects to the negative pressure pump housing 5. A negative pressure pump is provided inside the negative pressure pump housing 5 and is connected to the disposable hose 2 extending out of the centrifuge tube 4 through the disposable hose 2. A switch 6 is also provided on the negative pressure pump housing 5 to control the working state of the negative pressure pump.

[0030] In this embodiment, a negative pressure pump frame 8 is also provided at the bottom of the negative pressure pump housing 5. The negative pressure pump frame 8 is an open design that can support the negative pressure pump housing 5.

[0031] In this embodiment, the negative pressure pump housing 5 is also provided with a grip 7, which is made of rubber to prevent the negative pressure pump housing 5 from slipping from the user's hand during use.

[0032] In this embodiment, the outer surface of the centrifuge tube 4 is also provided with scale lines for observing the capacity inside the centrifuge tube.

[0033] In this embodiment, the disposable tubing 2 inserted into the centrifuge tube 4 is located near the bottom of the centrifuge tube 4 to prevent splashing inside the centrifuge tube 4 after being sucked in.

[0034] In this embodiment, the negative pressure pump is equipped with an internal power source to provide kinetic energy to the negative pressure pump.

[0035] This embodiment provides a collector for large-scale synchronization of placed micro-model organisms, one method of which is as follows:

[0036] Depending on the specific situation, the operator adds an appropriate amount of solution required for sample collection to the disposable centrifuge tube 4, assembles all components of the equipment, places the negative pressure pump on the negative pressure pump holder 8, places the biological sample to be collected under the microscope, holds the disposable pipette tip 1 with one hand for collection, and adjusts the position of the sample under the eyepiece as needed with the other hand. If the sample does not require frequent adjustment under the eyepiece, the operator can also choose to lift the negative pressure pump housing 5 with one hand and hold the disposable pipette tip 1 with the other hand for collection.

[0037] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present utility model. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.

Claims

1. A collector for large-scale synchronization of placed micro-organism model organisms, characterized by: Including disposable suction head (1), centrifuge tube (4) and negative pressure pump shell (5) connected in turn, disposable suction head (1), centrifuge tube (4) and negative pressure pump shell (5) are connected through disposable hose (2), the opening of centrifuge tube (4) is provided with rubber plug (3), centrifuge tube (4) is sealed by the rubber plug (3), the rubber plug (3) is provided with two openings, for fixing disposable hose (2), one of the openings disposable hose (2) is inserted into the centrifuge tube (4), the other opening disposable hose (2) is connected with the outside of the centrifuge tube (4) and the negative pressure pump shell (5); The inside of the negative pressure pump shell (5) is provided with a negative pressure pump, and a switch (6) is further provided on the negative pressure pump shell (5), and the switch (6) is used for controlling the working state of the negative pressure pump.

2. The collector for large-scale synchronization of ciliates according to claim 1, wherein: The bottom of the negative pressure pump shell (5) is further provided with a negative pressure pump frame (8), and the negative pressure pump frame (8) is designed as an open type and supports the negative pressure pump shell (5).

3. The collector for large-scale synchronization of ciliates according to claim 1, wherein: The negative pressure pump shell (5) is further provided with a holding place (7), and the holding place (7) is made of rubber.

4. The collector for large-scale synchronization of ciliates according to claim 1, wherein: The outer surface of the centrifuge tube (4) is further provided with a scale line.

5. The collector for large-scale synchronization of ciliates according to claim 1, wherein: The end of the disposable hose (2) in the centrifuge tube (4) is close to the bottom of the centrifuge tube (4).

6. The device for large-scale synchronization of ciliates according to claim 1, wherein: The negative pressure pump is provided with an internal power supply.

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