Droplet generation equipment and detection system

Abstract

The utility model provides liquid drop generation equipment and a detection system. The liquid drop generating equipment comprises a first carrying table, a pressurizing assembly, a pop-up piece and a sealing piece, the pressurizing assembly comprises a pressurizing piece and a driving part, the pressurizing piece is connected to the driving part, the driving part drives the pressurizing piece to move between a first position and a second position, the pressurizing piece is closer to the first carrying table when in the second position, and the pop-up piece is located between the first carrying table and the second carrying table when in the second position. The sealing piece is abutted between the micro-fluidic chip assembly and the pressurizing piece; the ejection piece is connected to the pressurizing piece, and / or the ejection piece is connected to the micro-fluidic chip assembly, and when the pressurizing piece moves from the second position to the first position, the ejection piece pushes one of the micro-fluidic chip assembly and the pressurizing piece to separate the micro-fluidic chip assembly from the pressurizing piece, so that the problem that when the pressurizing piece moves to the first position, the micro-fluidic chip assembly cannot be separated from the pressurizing piece due to the influence of factors such as a sealing piece can be solved. The problem that the micro-fluidic chip assembly moves along with the pressurizing piece is solved, the difficulty of installing the micro-fluidic chip assembly in the liquid drop generation equipment is reduced, and the reliability of a detection system is improved.

Description

Technical Field

[0001] This application relates to the field of microfluidics, and in particular to a droplet generation device and detection system. Background Technology

[0002] Existing droplet generation devices in digital polymerase chain reaction (dPCR) detection systems typically include a pressurizing component and a sealing component. During droplet generation, the pressurizing component is used to pressurize the chip within the device. When the pressurizing component returns to its original position after pressurization, the chip can easily move along with it due to factors such as the sealing component, affecting the reliability of subsequent detection processes. To overcome this problem, existing droplet generation devices generally require the operator to manually fix the chip beforehand, resulting in high labor costs and hindering automation of the detection equipment. Utility Model Content

[0003] This application provides a droplet generation device and a detection system. By setting up a pop-out component, the problem of the microfluidic chip assembly moving together with the resetting process of the pressurized component can be solved, ensuring the reliability of the detection system. It can also reduce the difficulty of installing the microfluidic chip assembly in the droplet generation device, which is conducive to reducing manual intervention and improving the automation level of the detection system.

[0004] In a first aspect, this application provides a droplet generation device. The droplet generation device includes a first stage, a pressurizing component, a pop-out component, and a sealing component. The first stage is used to place a microfluidic chip assembly. The pressurizing component pressurizes the microfluidic chip assembly placed on the first stage to generate droplets. The pressurizing component includes a pressurizing element and a driving component. The pressurizing element is connected to the output end of the driving component. The driving component drives the pressurizing element to move between a first position and a second position. In the second position, the pressurizing element is closer to the first stage than in the first position. In the second position, the sealing component abuts against the microfluidic chip assembly and the pressurizing element. The pop-out component is connected to the pressurizing element, and / or the pop-out component is connected to the microfluidic chip assembly. When the pressurizing element moves from the second position to the first position, the pop-out component pushes one of the microfluidic chip assembly and the pressurizing element to separate them.

[0005] In one possible implementation, the pop-up component has the ability to elastically deform.

[0006] In one possible implementation, in the second position, the ejector abuts between the microfluidic chip assembly and the pressurizing element, and the ejector and the seal are spaced apart.

[0007] In one possible implementation, both the pop-out member and the seal are fixed to the side of the pressure member facing the first platform. When the pressure member is in the first position, the pop-out member protrudes relative to the seal.

[0008] In one possible implementation, the pop-out component includes a first magnetic component and a second magnetic component, the first magnetic component being fixed to the pressure component and the second magnetic component being fixed to the microfluidic chip assembly;

[0009] As the pressurizing element moves from the second position toward the first position, the repulsive force between the first magnetic element and the second magnetic element is used to push the microfluidic chip assembly to separate from the pressurizing element.

[0010] In one possible implementation, the droplet generation device further includes a base and a second stage, the second stage being used to place at least one carrier tube for collecting droplets generated by the microfluidic chip assembly; the first stage and the second stage are separately arranged and are movably connected to the base respectively.

[0011] When the pressurizing component moves toward the first stage, it drives the first stage to move toward the side of the second stage, so that the droplet outlet of the microfluidic chip assembly extends into the corresponding carrier tube.

[0012] In one possible implementation, the droplet generating device further includes a first lateral movement component, which includes a first lateral movement motor and a first lateral movement member. A pressure member is connected to the first lateral movement member, and the first lateral movement motor drives the first lateral movement member to move, thereby causing the pressure member to move toward the first platform.

[0013] In one possible implementation, the droplet generation device includes a sample loading platform and a scheduling component, the scheduling component being used to transfer the microfluidic chip assembly from the sample loading platform to a first stage, and / or to remove the microfluidic chip assembly from the first stage.

[0014] Secondly, embodiments of this application provide a detection system. The detection system includes a microfluidic chip assembly and a droplet generation device as described in any of the above embodiments, with the microfluidic chip assembly placed on a first stage.

[0015] In one possible implementation, the microfluidic chip assembly includes a microfluidic chip and a connecting frame, with the microfluidic chip fixed to the connecting frame, and in a second position, a pop-up element corresponding to the connecting frame.

[0016] In this embodiment, a pop-out component pushes one of the microfluidic chip assembly and the pressurizing component to separate them, solving the problem of the microfluidic chip assembly moving with the pressurizing component due to factors such as seals. This ensures that the microfluidic chip assembly remains on the first platform after the venting and pressurization process is completed, guaranteeing the reliability of the droplet generation process. Furthermore, in this solution, the microfluidic chip assembly can be directly mounted on the first platform, eliminating the need for additional fixing components on the first platform as in existing technologies. This ensures the reliability of the droplet generation process while reducing manufacturing costs. The process of installing the microfluidic chip assembly onto the first platform is simple, easy to operate, and has low difficulty. Attached Figure Description

[0017] Figure 1 This is a simplified schematic diagram illustrating the application of a droplet generation device provided in an embodiment of this application;

[0018] Figure 2 yes Figure 1 The diagram shows a partial structure of the droplet generation device, the microfluidic chip assembly, and the carrier tube in some embodiments.

[0019] Figure 3 yes Figure 2 A schematic diagram of part of the droplet generation device shown from another perspective;

[0020] Figure 4 yes Figure 2 The diagram shows a partial structure of the pressurization component and its interaction with the microfluidic chip component.

[0021] Figure 5 yes Figure 4 The diagram shows a partial structure of the pressurization component and an exploded view of the microfluidic chip component.

[0022] Figure 6 yes Figure 4 The diagram shown illustrates the application of the pressurization component in one operating state.

[0023] Figure 7 yes Figure 4 The diagram shows the application of the pressurization component in another operating state;

[0024] Figure 8 yes Figure 1 The diagram shows a partial structure of the droplet generation device, the microfluidic chip assembly, and the carrier tube in some other embodiments;

[0025] Figure 9 yes Figure 8 The diagram shows the structure of the droplet generation device in another operating state.

[0026] Figure 10 This is a simplified structural diagram of a detection system provided in an embodiment of this application.

[0027] 1-Detection system, 1000-Droplet generation device, 100-House, 11-Base, 12-Side wall, 121-Transfer window, 1211-First transfer window, 1212-Second transfer window, 21-First stage, 211-Placement hole, 212-Stop component, 213-Reset component, 22-Second stage, 300-Pressure assembly, 31-Pressure component, 32-Drive component, 321-Lifting motor, 33-Sealing component, 34-Ejector component, 35-Bearing seat, 351-Through groove, 352-Linkage block, 400-Second transverse component, 41-Second transverse component, 42-Second transverse motor, 500-Linkage assembly, 51- Linkage, 511 - First end of link, 512 - Middle part of link, 513 - Second end of link, 600 - Sample loading platform, 61 - Drawer, 700 - Scheduling component, 2000 - Microfluidic chip component, 201 - Microfluidic chip, 201a - Generation cell, 2011 - Input port, 2011a - Aqueous phase input port, 2011b - Oil phase input port, 2012 - Aqueous phase storage cell, 2013 - Oil phase storage cell, 2014 - Droplet outlet, 202 - Connecting frame, 3000 - Carrier tube, 4000 - Sample pretreatment equipment, 4001 - First transfer track, Z - First direction, X - Second direction, Y - Third direction. Detailed Implementation

[0028] The embodiments of this application are described below with reference to the accompanying drawings.

[0029] In the description of the embodiments of this application, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, "connection" can be a detachable connection or a non-detachable connection; it can be a direct connection or an indirect connection through an intermediate medium.

[0030] The directional terms mentioned in the embodiments of this application, such as "upper", "lower", "inner", "outer", "bottom", "side", etc., are only for reference to the direction of the accompanying drawings. Therefore, the directional terms used are for better and clearer explanation and understanding of the embodiments of this application, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.

[0031] In the embodiments of this application, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" and "second" may explicitly or implicitly include one or more of that feature.

[0032] Please refer to the following: Figures 1 to 3 , Figure 1 This is a simplified schematic diagram illustrating the application of a droplet generation device 1000 provided in an embodiment of this application. Figure 2 yes Figure 1 The diagram shows a partial structure of the droplet generation device 1000, the microfluidic chip assembly 2000, and the carrier tube 3000 in some embodiments. Figure 3 yes Figure 2 A schematic diagram of part of the structure of the droplet generation device 1000 shown from another perspective.

[0033] It is understood that, for ease of description, the definitions in the embodiments of this application are as follows. Figure 1 The droplet generating device 1000 shown has a first direction Z, a second direction X, and a third direction Y. The first direction Z can be parallel to the height direction of the droplet generating device 1000, the second direction X can be parallel to the width direction of the droplet generating device 1000, and the third direction Y can be parallel to the length direction of the droplet generating device 1000. Any two of the first direction Z, the second direction X, and the third direction Y are set at an angle. For example, any two of the first direction Z, the second direction X, and the third direction Y can be perpendicular to each other.

[0034] The droplet generating device 1000 includes a housing 100, a placement stage, and a pressurizing assembly 300. The placement stage can be used to place the generating module, and the pressurizing assembly 300 can apply pressure to the generating module placed on the placement stage to generate droplets.

[0035] For example, the housing 100 may include a base 11 and four side walls 12. The four side walls 12 may be connected to the four sides of the base 11 respectively. The base 11 and the four side walls 12 may together enclose the internal space of the housing 100. Structural components such as the placement platform and the pressurization assembly 300 in the droplet generation device 1000 may be located in the internal space of the housing 100.

[0036] For example, the placement stage may include a first stage 21 and a second stage 22 separately arranged. The generation module may include a microfluidic chip assembly 2000 and at least one carrier tube 3000. The first stage 21 is used to place the microfluidic chip assembly 2000, and the second stage 22 is used to place at least one carrier tube 3000. The pressurization component 300 can vent and pressurize the microfluidic chip assembly 2000 placed on the first stage 21 to generate droplets. The carrier tube 3000 is used to collect the droplets generated by the microfluidic chip assembly 2000. The separation of the first stage 21 and the second stage 22 may be such that the first stage 21 and the second stage 22 are spaced apart in a first direction Z, and / or, the first stage 21 and the second stage 22 are spaced apart in a second direction X, and / or, the first stage 21 and the second stage 22 may be spaced apart in a third direction Y. This embodiment of the application does not limit this.

[0037] It is understood that the first stage 21 and the second stage 22 are set separately. In other words, in the embodiments of this application, the first stage 21 and the second stage 22 are two independent structural components within the droplet generation device 1000. That is, the microfluidic chip assembly 2000 and the carrier tube 3000 are two independent structural components. This helps to reduce the design difficulty and manufacturing cost of making the microfluidic chip assembly 2000 and the carrier tube 3000 into an integrated structure. Furthermore, the correspondence between the specifications of the microfluidic chip assembly 2000 and the number of carrier tubes 3000 can be flexibly set according to actual needs. In some other embodiments, the placement stage may include a first stage 21 but not a second stage 22. The placement stage (i.e., the first stage 21) may be used to place the generation module. The generation module may include a microfluidic chip assembly 2000 and at least one carrier tube 3000. The carrier tube 3000 may be fixedly connected to the microfluidic chip assembly 2000. In other words, the generation module may also be an integrated structure formed by the carrier tube 3000 and the microfluidic chip assembly 2000. The pressurization component 300 may be used to pressurize the generation module (i.e., the microfluidic chip assembly 2000) placed on the first stage 21. The pressurization process may refer to the pressurization process described below with the first stage 21 and the second stage 22 set separately as an example. This embodiment of the application will not repeat the details.

[0038] Please refer to the following: Figure 1 , Figure 4 and Figure 5 , Figure 4 yes Figure 2 The diagram shows a partial structure of the pressurization component 300 and its interaction with the microfluidic chip component 2000. Figure 5 yes Figure 4 The diagram shows a partial structure of the pressurization component 300 and an exploded view of the microfluidic chip component 2000.

[0039] It is understood that the microfluidic chip assembly 2000 may include a microfluidic chip 201, and the microfluidic chip 201 may include at least one generation cell 201a. Each generation cell 201a may include an inlet 2011, an aqueous phase storage cell 2012, an oil phase storage cell 2013, and a droplet outlet 2014. The aqueous phase storage cell 2012 and the oil phase storage cell 2013 may be arranged at intervals along the second direction X. One end of the aqueous phase storage cell 2012 is provided with an aqueous phase inlet 2011a (the inlet of the aqueous phase storage cell 2012 is referred to as the aqueous phase inlet 2011a for distinction). The aqueous phase sample is injected into the aqueous phase storage cell 2012 through the aqueous phase inlet 2011a. The oil phase storage cell 2012... One end of 013 is provided with an oil phase inlet 2011b (the inlet 2011 of the oil phase storage tank 2013 is referred to as the oil phase inlet 2011b for distinction). The oil phase sample is injected into the oil phase storage tank 2013 through the oil phase inlet 2011b. The droplet outlet 2014 connects the other end of the aqueous phase storage tank 2012 and the other end of the oil phase storage tank 2013. The samples in the aqueous phase storage tank 2012 and the oil phase storage tank 2013 are squeezed and flowed under the pressure of the pressurizing component 300 to form "water-in-oil" micro-droplets. The generated micro-droplets can flow out of the microfluidic chip component 2000 through the droplet outlet 2014 and into the carrier tube 3000 (e.g., Figure 1 (As shown) corresponds to the droplet outlet 2014 (e.g.) Figure 4 As shown, in the configuration, for example, the carrier tube 3000 and the droplet outlet 2014 are arranged at intervals along the first direction Z, or, when the droplet outlet 2014 extends into the carrier tube 3000, the droplets flow into the carrier tube 3000 through the droplet outlet 2014. According to the principle of "like dissolves like," the phase that is easily soluble in water belongs to the aqueous phase, and the phase that is not easily soluble in water belongs to the oil phase. When there are multiple generation tanks 201a, the multiple generation tanks 201a can be arranged in a row and column arrangement; this embodiment does not limit this arrangement.

[0040] For example, the microfluidic chip assembly 2000 may further include a connecting frame 202, which may surround at least a portion of the outer periphery of the microfluidic chip 201. The microfluidic chip 201 may be fixed within the connecting frame 202 by means of snap-fit ​​or other methods. By providing the connecting frame 202, it is beneficial to facilitate the handling process of the microfluidic chip assembly 2000, avoiding direct contact between the microfluidic chip assembly 2000 and the microfluidic chip 201 by structural components used for transport within the droplet generation device 1000 (the scheduling component 700 described later) during the transfer of the microfluidic chip assembly 2000, which could damage the microfluidic chip 201 and thus affect the reliability of droplet generation by the droplet generation device 1000. It is understood that the microfluidic chip 201 may be a single, larger chip, or it may be composed of multiple smaller chips joined together. Multiple smaller chips may be integrated into a single structure through the connecting frame 202. This application embodiment does not limit this.

[0041] Please refer to it again. Figures 1 to 3 The pressurization assembly 300 may include a pressurization member 31 and a driving member 32. The pressurization member 31 is connected to the output end of the driving member 32. The driving member 32 drives the pressurization member 31 to move between a first position and a second position. In the second position, the pressurization member 31 is closer to the first stage 21 than in the first position. In the second position, the pressurization member 31 presses against the microfluidic chip assembly 2000.

[0042] For example, the drive component 32 may include a lifting motor 321 and a lifting element ( Figure 2 and Figure 3 (Not shown in the image), the pressure member 31 can be connected to one end of the lifting member. The lifting motor 321 is used to drive the lifting member to move the pressure member 31 up and down along the first direction Z, so that the pressure member 31 moves between the first position and the second position. The lifting member can be considered as the output end of the driving component 32. The lifting member can be a structural component such as a lead screw, but this embodiment of the application does not limit it.

[0043] It is understood that the pressurizing element 31 may include at least two vents. Figure 2 and Figure 3 (Not shown in the image) When the pressurizing component 31 is in the first position, the vent and the inlet 2011 are spaced apart in the first direction Z. When the lifting motor 321 moves the pressurizing component 31 from the first position toward the second position, the distance between the vent and the inlet 2011 decreases. When the pressurizing component 31 reaches the second position, the two vents can be connected to the oil phase inlet 2011b and the water phase inlet 2011a respectively. The pressurizing component 31 can pressurize the oil phase storage tank 2013 and the water phase storage tank 2012 through the vents to generate droplets.

[0044] The distance between two adjacent vents can be equal to the distance between two corresponding input ports 2011. The number of vents can be less than, greater than or equal to the number of input ports 2011. When the number of vents is the same as the number of input ports 2011, the vents and input ports 2011 are set in a one-to-one correspondence. This application embodiment does not limit the specific number or arrangement of vents.

[0045] Please refer to it again. Figure 2 , Figure 4 and Figure 5 The droplet generation device 1000 may include a seal 33, which may be located on the side of the pressure member 31 facing the first stage 21. The seal 33 may be connected to or separate from the pressure member 31. When the pressure member 31 is in a second position, the seal 33 abuts against the pressure member 31 and at least a portion of the microfluidic chip assembly 2000. In some examples, the seal 33 may include a sealing portion and a through-hole. The through-hole may penetrate the sealing portion in a first direction Z and may correspond to the inlet port 2011, allowing the inlet port 2011 to be exposed relative to the seal 33 for communication with a vent. When the pressure member 31 is in the second position, the sealing portion surrounds the outer periphery of at least a portion of the inlet port 2011 within the microfluidic chip assembly 2000 and is located between the pressure member 31 and a portion of the microfluidic chip assembly 2000.

[0046] The sealing element 33 can be made of rubber materials such as silicone. By positioning the sealing element 33 on the side of the pressurizing element 31 facing the first stage 21, in other words, when the pressurizing element 31 is in the second position, the sealing element 33 can abut against the pressurizing element 31 and at least part of the microfluidic chip assembly 2000. The sealing element 33 can play a buffering role to prevent the pressurizing element 31 from damaging the microfluidic chip assembly 2000. Furthermore, the sealing element 33 surrounds the inlet 2011 and can also play a sealing role to improve the sealing performance of the chamber formed by the connection between the vent and the inlet 2011, preventing outside air from entering the generation pool 201a of the microfluidic chip assembly 2000 through the gap between the vent and the inlet 2011, thus affecting the reliability of the droplet generation process.

[0047] In some examples, the seal 33 can be fixed to the surface of the pressure member 31 facing the first stage 21. The through holes of the seal 33 can be configured one-to-one with the vents of the pressure member 31. It is understood that when the pressure member 31 is used to pressurize the microfluidic chip assembly 2000, compared to having a seal 33 on each microfluidic chip assembly 2000, this embodiment of the application, by setting the seal 33 on the pressure member 31, allows the seal 33 to separate from the microfluidic chip assembly 2000 with the movement of the pressure member 31 after pressurization. The seal 33 can be reused, which helps reduce testing costs. In other embodiments, the seal 33 can also be fixed to the surface of the microfluidic chip assembly 2000 away from the first stage 21, that is, located on the side of the first stage 21 facing the pressure member 31. The through holes can be configured one-to-one with the input ports 2011 of the microfluidic chip assembly 2000. This embodiment of the application does not limit this.

[0048] Please refer to the following: Figure 2 , Figure 6 and Figure 7 , Figure 6 yes Figure 4 The diagram shows the application of the pressurization component 300 in one working state. Figure 7 yes Figure 4 The diagram shows the application of the pressurizing component 300 in another operating state. For example... Figure 6 The pressure member 31 shown is located in the second position, as... Figure 7 The pressure member 31 shown is located between the second position and the first position.

[0049] In some embodiments, the droplet generation device 1000 may include a pop-out member 34, which may be connected to a pressure member 31, and / or the pop-out member 34 may be connected to a microfluidic chip assembly 2000. When the pressure member 31 moves from a second position to a first position, the pop-out member 34 pushes one of the microfluidic chip assembly 2000 and the pressure member 31 to separate them.

[0050] It is understandable that when the pressurizing component 31 is in the second position and undergoing the venting and pressurization process, the sealing component 33 is located between the pressurizing component 31 and at least part of the microfluidic chip assembly 2000. When the pressurizing component 31 completes the venting and pressurization process and moves from the second position towards the first position, factors such as the sealing component 33, for example, due to continuous force during pressurization, may cause the microfluidic chip assembly 2000 to adhere to the pressurizing component 31, leading to the microfluidic chip assembly 2000 moving and separating from the first stage 21 along with the movement of the pressurizing component 31. In this embodiment, by providing a pop-out component 34 to push the microfluidic chip assembly 2000 and one of the pressurizing components 31 to separate them, the influence of factors such as the sealing component 33 on the microfluidic chip assembly 2000 is resolved. This ensures that the microfluidic chip assembly 2000 remains on the first stage 21 after the venting and pressurization process is completed, thus ensuring the reliability of the droplet generation process. Furthermore, by setting the pop-out component 34, the microfluidic chip assembly 2000 can be directly mounted on the first stage 21, eliminating the need for additional fixing components on the first stage 21 in the prior art for fixing the microfluidic chip assembly 2000. This makes the droplet generation device 1000 of this embodiment reliable while having a low manufacturing cost, and the process of installing the microfluidic chip assembly 2000 onto the first stage 21 is simple, easy to operate, and has low difficulty.

[0051] For example, the ejector 34 can be a structural component with elastic deformation capabilities, such as a spring or sheet. Combined with... Figure 6 and Figure 7 As shown, when the pressure member 31 is in the second position, the pressure member 31 presses against the microfluidic chip assembly 2000, and the pop-out member 34 is compressed. During the process of the pressure member 31 moving from the second position to the first position, the deformation recovery force of the pop-out member 34 can push the microfluidic chip assembly 2000 to move away from the pressure member 31, so as to separate the microfluidic chip assembly 2000 from the pressure member 31.

[0052] In some examples, when the pressure member 31 is in the second position, the ejector 34 can be spaced apart from the seal 33. For example, when the pressure member 31 is in the second position, the ejector 34 can surround the outer periphery of the seal 33 and be spaced apart from the seal 33 in both the second direction X and the third direction Y; or, when the pressure member 31 is in the second position, the ejector 34 can be located on one side of the pressure member 31 and closer to the edge of the pressure member 31 than the seal 33, and be spaced apart from the seal 33 in either the second direction X or the third direction Y. In other embodiments, the pressure member 31 and the seal 33 can also be spaced apart in the first direction Z, which is not limited in this application. By setting the ejector 34 and the seal 33 to be spaced apart when the pressure member 31 is in the second position, the effect of the adhesion of the seal 33 on the operation of the ejector 34 can be reduced.

[0053] In some examples, the pop-out member 34 can be fixed to the side of the pressure member 31 facing the first stage 21 and is positioned corresponding to the connection frame 202 of the microfluidic chip assembly 2000. When the pressure member 31 is in the second position, the pop-out member 34 presses against the connection frame 202 and the pressure member 31. By fixing the pop-out member 34 to the pressure member 31, the pop-out member 34 can still be reused when the microfluidic chip assembly 2000 is replaced within the droplet generation device 1000. Furthermore, the pop-out member 34 being positioned corresponding to the connection frame 202 of the microfluidic chip assembly 2000 can prevent the pop-out member 34 from damaging the microfluidic chip 201, thus improving the reliability of the droplet generation process in the droplet generation device 1000. In other embodiments, the pop-out member 34 can also be fixed to the connection frame 202, that is, when the microfluidic chip assembly 2000 is located on the first stage 21, the pop-out member 34 is located on the side of the pressure member 31 facing the first stage 21. This application does not limit this aspect.

[0054] In some examples, when the pressure member 31 is in the first position, the pop-out member 34 protrudes relative to the seal member 33. It is understood that both the pop-out member 34 and the seal member 33 are fixed to the pressure member 31. For example, when the deformation restoring force of the pop-out member 34 is used to push the microfluidic chip assembly 2000 apart from the pressure member 31, by setting the pop-out member 34 to protrude relative to the seal member 33, during the process of the pressure member 31 driving the pop-out member 34 and the seal member 33 towards the first stage 21, the pop-out member 34 can contact the microfluidic chip assembly 2000 first compared to the seal member 33. This is beneficial for fully utilizing the movement path of the pressure member 31, increasing the deformation of the pop-out member 34, and improving the effect of the pop-out member 34. Furthermore, this also avoids situations where, if the seal 33 protrudes more than the pop-out member 34, the pop-out member 34 may not be able to contact the connecting frame 202 due to the obstruction of the seal 33, or the deformation of the pop-out member 34 may be too small, resulting in insufficient deformation recovery force to push the microfluidic chip assembly 2000 apart from the pressure member 31. In some other embodiments, the pop-out member 34 may also be flush with the seal 33, or the seal 33 may protrude more than the pop-out member 34; this application does not limit this aspect.

[0055] In some other embodiments, the pop-out member 34 may not have elastic deformation capability, and the pop-out member 34 may also have other implementation structures. For example, the pop-out member 34 may also be a split structure, and the pop-out member 34 may include a first magnetic member and a second magnetic member. The first magnetic member is fixed to the pressure member 31, and the second magnetic member is fixed to the microfluidic chip assembly 2000. When the pressure member 31 moves from the second position to the first position, the repulsive force between the first magnetic member and the second magnetic member is used to push the microfluidic chip assembly 2000 to separate from the pressure member 31. The first magnetic member may be fixed to the side of the pressure member 31 facing the first stage 21, or at least a portion of the first magnetic member may be embedded in the pressure member 31; the second magnetic member may be fixed to the side of the connecting frame 202 facing the pressure member 31, or at least a portion of the second magnetic member may be embedded in the connecting frame 202. This application embodiment does not limit this.

[0056] Please refer to it again. Figure 2 and Figure 3 In some embodiments, the droplet generation device 1000 may include a first lateral movement component ( Figure 2 and Figure 3(Not shown in the image), the pressurizing component 300 may include a support base 35, a lifting motor 321 fixed to the support base 35, the support base 35 may have a through groove 351 through which the lifting component can pass, and the pressurizing component 31 located on the side of the support base 35 away from the lifting motor 321. The first lateral movement component may include a first lateral movement motor and a first lateral movement member, the support base 35 is fixedly connected to the first lateral movement member, and the first lateral movement motor is fixedly connected to the base 11. The first lateral movement motor is used to drive the first lateral movement member to move the support base 35 and the pressurizing component 31 along the second direction X. The first lateral movement member may be a lead screw or a belt on one side of a pulley assembly, etc., and this embodiment of the application does not limit this.

[0057] Understandably, in this embodiment, the movement process of the pressurizing component 31 moving to the target position (i.e., the second position) may include: a first lateral motor driving a first lateral component to move the pressurizing component 31 along the second direction X to above the first platform 21, at which point the pressurizing component 31 moves from the initial position to the first position, and the pressurizing component 31 and the first platform 21 are spaced apart along the first direction Z; and a lifting motor 321 driving a lifting component to move the pressurizing component 31 from the first position to the second position along the first direction Z. By setting the first lateral component, it is beneficial to improve the flexibility of the relative position of the pressurizing component 31 and the first platform 21, so that before the pressurizing component 300 vents and pressurizes the microfluidic chip component 2000, the pressurizing component 300 and the first platform 21 can be spaced apart in the second direction X, which provides more operating space for the process of installing the microfluidic chip component 2000 onto the first platform 21 and reduces the installation difficulty of the microfluidic chip component 2000.

[0058] Furthermore, by setting a first lateral movement component, when the number of vents on the pressurizing component 31 is less than the number of input ports 2011 of the microfluidic chip assembly 2000, the first lateral movement motor can drive the first lateral movement component to move the pressurizing component 31 in the second direction X, thereby realizing the pressurization process of the other input ports 2011 of the microfluidic chip assembly 2000 in the second direction X. This improves the reusability of the pressurizing component 31 in the droplet generation device 1000 and the flexibility of the venting and pressurization process, enabling the droplet generation device 1000 to adapt to more microfluidic chip assemblies 2000 of different specifications. In some other embodiments, the droplet generation device 1000 may not include the first lateral movement component. The pressurizing component 31 can be fixed to the first stage 21 by the support 35. The initial position of the pressurizing component 31 can be a first position. When venting and pressurization are required, the pressurizing component 31 moves from the first position to a second position along the first direction Z. This application embodiment does not limit this.

[0059] Please refer to it again. Figures 1 to 3The first stage 21 and / or the second stage 22 are movably connected to the base 11 of the housing 100, and their relative positions can be flexibly adjusted according to different needs. For example, when it is necessary to load the microfluidic chip assembly 2000 onto the first stage 21 and / or to load the carrier tube 3000 onto the second stage 22, the first stage 21 and the second stage 22 can be set separately to provide more operating space for the process of installing the microfluidic chip assembly 2000 onto the first stage 21 and the process of installing the carrier tube 3000 onto the second stage 22; as another example, when it is necessary to collect droplets, the second stage 22 can be located below the first stage 21 so that the carrier tube 3000 can correspond to the droplet outlet 2014 of the microfluidic chip assembly 2000 (e.g., ...). Figure 4 (As shown). When the carrier tube 3000 corresponds to the droplet outlet 2014 of the microfluidic chip assembly 2000, the carrier tube 3000 and the droplet outlet 2014 can be spaced apart in the first direction Z, or the droplet outlet 2014 can also extend into the carrier tube 3000.

[0060] For example, the first stage 21 can be spaced apart from the base 11 in the first direction Z, and the first stage 21 is movably connected to the base 11 in the first direction Z. The second stage 22 can be movably connected to the base 11 in the second direction X. By moving the second stage 22, the carrier tube 3000 can be moved to the corresponding droplet outlet 2014. Then, by moving the first stage 21, the microfluidic chip assembly 2000 can be moved towards the base 11, so that the droplet outlet 2014 of the microfluidic chip assembly 2000 extends into the carrier tube 3000, which helps to improve the reliability of the droplet collection process of the carrier tube 3000.

[0061] The first stage 21 may be provided with a placement hole 211, which can penetrate the first stage 21 in the first direction Z. The droplet outlet 2014 can pass through the placement hole 211 to improve the positioning accuracy of the microfluidic chip assembly 2000 when it is installed on the first stage 21. In some other embodiments, the microfluidic chip assembly 2000 may also be placed on the first stage 21 in other ways, which is not limited in this application.

[0062] In some examples, the droplet generating mechanism may include a stop 212, which may be fixedly connected to the base 11. The first stage 21 is movably connected to the stop 212, and the first stage 21 may be located on the side of the stop 212 away from the base 11. It is understood that when the first stage 21 is subjected to pressure in the first direction Z, the first stage 21 will move toward the base 11. By setting the stop 212, when the first stage 21 abuts against the stop 212, the stop 212 can block the movement of the first stage 21, thereby limiting the distance the first stage 21 moves in the first direction Z and preventing damage to the carrier tube 3000 on the second stage 22 when the first stage 21 is pressed down excessively.

[0063] The dimension of the stop component 212 in the first direction Z can be greater than or equal to the dimension of the second stage 22 in the first direction Z. It is understood that when the first stage 21 is not under pressure, the first stage 21 and the stop component 212 are spaced apart in the first direction Z. By setting the distance between the second stage 22 and the base 11 to be less than or equal to the distance between the stop component 212 and the base 11, the stop component 212 can act as a limit while ensuring that the second stage 22 can move smoothly below the first stage 21, thus improving the reliability and safety of the coordination of the various components of the droplet generation device 1000.

[0064] In some examples, the droplet generating device 1000 further includes a second lateral movement component 400. The second lateral movement component 400 may include a second lateral movement member 41 and a second lateral movement motor 42. The second lateral movement motor 42 is used to drive the second lateral movement member 41 to move along a second direction X. The second platform 22 may be fixedly connected to the second lateral movement member 41 so as to move with the second lateral movement member 41 relative to the base 11 in the second direction X. The second lateral movement member 41 may be a structure such as a lead screw or a belt on one side of a pulley assembly; this embodiment of the application does not limit this.

[0065] In this embodiment, the structure of the droplet generation device 1000 is described using the example of a first stage 21 movably connected to the base 11 along the first direction Z and a second stage 22 movably connected to the base 11 along the second direction X. In other embodiments, the droplet generation device 1000 may have other structures. For example, the second lateral movement member 41 may be connected to the first stage 21, the second stage 22 may be fixedly connected to the base 11, and the first stage 21 may be movably connected to the base 11 in both the first direction Z and the second direction X. Through the lateral and vertical movements of the first stage 21, the droplet outlet 2014 of the microfluidic chip assembly 2000 (e.g., ...) is realized. Figure 4 As shown, it extends into the corresponding bearing tube 3000. This application embodiment does not limit this, and the specific movement process can be set as needed. This application embodiment does not limit this.

[0066] For example, the droplet generating device 1000 may include a control unit, which is the control center of the droplet generating device 1000. The control unit can connect to various components within the droplet generating device 1000 using various interfaces and lines. For example, the control unit can be electrically and communicatively connected to structural components such as the first lateral movement component, the pressurization component 300, and the second lateral movement component 400, as well as their related components, to coordinate the coordinated work of various components within the droplet generating device 1000 and improve the automation level of the droplet generating device 1000.

[0067] Please refer to the following: Figures 1 to 4 In this embodiment, when the pressurizing component 300 moves toward the first stage 21 (i.e., when the first traverse motor and / or the lifting motor 321 are working), it can drive the first stage 21 to move toward the side of the second stage 22, so that the droplet outlet 2014 of the microfluidic chip component 2000 extends into the corresponding carrier tube 3000. This eliminates the need for an additional drive mechanism to drive the movement of the first stage 21 in the first direction Z, thus saving manufacturing costs for the droplet generation device 1000. In other embodiments, an additional drive mechanism may be provided to drive the first stage 21 to move along the first direction Z; this embodiment does not limit this.

[0068] Specifically, the control unit can first control the first transverse motor to work, so that the pressure member 31 moves with the first transverse member and the carrier 35 to move above the microfluidic chip assembly 2000. Then, it controls the lifting motor 321 to work, so that the pressure member 31 moves with the lifting member along the first direction Z toward the first platform 21. Since the first platform 21 is movably connected to the base 11, when the pressure member 31 moves along the first direction Z to contact the microfluidic chip assembly 2000, the pressure member 31 will provide downward pressure to the first platform 21, thereby pushing the first platform 21 to drive the microfluidic chip assembly 2000 to move downward, so that the droplet outlet 2014 of the microfluidic chip assembly 2000 extends into the corresponding carrier tube 3000.

[0069] It is understood that the starting order of the first transverse motor and the second transverse motor 42 is not limited in the embodiments of this application. In other words, in the embodiments of this application, the control unit can first control the second transverse motor 42 to work, so as to drive the second platform 22 to move below the first platform 21, and then control the first transverse motor to work, so as to move the pressurizing component 31 above the first platform 21. When the pressurizing component 31, the first platform 21 and the second platform 22 are arranged along the first direction Z, the control unit then controls the lifting motor 321 to work, so as to move the pressurizing component 31 to the second position, so as to realize the ventilation and pressurization process; or, the control unit can also first control the first transverse motor to work, so as to drive the pressurizing component 31 to move. Once the first platform 21 is above the second platform 21, the second transverse motor 42 is activated to drive the second platform 22 to move below the first platform 21. When the pressurizing component 31, the first platform 21, and the second platform 22 are arranged along the first direction Z, the control unit controls the lifting motor 321 to work, so that the pressurizing component 31 moves to the second position to realize the ventilation and pressurization process. Alternatively, the control unit can also control the second transverse motor 42 to start simultaneously with the first transverse motor. When the pressurizing component 31, the first platform 21, and the second platform 22 are arranged along the first direction Z, the control unit controls the lifting motor 321 to work, so that the pressurizing component 31 moves to the second position to realize the ventilation and pressurization process. The embodiments of this application do not limit this.

[0070] For example, the droplet generation device 1000 also includes a reset member 213, which is elastically connected between the first stage 21 and the base 11. After the pressurizing member 31 pressurizes the microfluidic chip assembly 2000, the pressurizing member 31 moves from the second position toward the first position (the pressurizing member 31 moves away from the base 11) along the first direction Z. The reset member 213 is used to push the first stage 21 to move away from the base 11.

[0071] Understandably, the reset member 213 can be a structural component with elastic deformation capabilities, such as a spring. When the pressurizing member 31 pressurizes the microfluidic chip assembly 2000, the first stage 21 is pushed towards the base 11, and the reset member 213 is compressed. When the pressurizing member 31 moves away from the base 11 along the first direction Z, the deformation restoring force of the reset member 213 can push the first stage 21 away from the base 11, so that the first stage 21 returns to its original position, improving the reusability of the droplet generation mechanism. Furthermore, by using the deformation restoring force of the reset member 213 as the driving force, compared to setting an additional driving mechanism to drive the first stage 21 back to its original position, the complexity of the internal structure and manufacturing cost of the droplet generation device 1000 are effectively reduced.

[0072] In the previous embodiment, when the pressurizing component 31 moves along the first direction Z, it pushes the first stage 21 to drive the microfluidic chip assembly 2000 to move downward. In some other embodiments, when the pressurizing component 300 moves along the second direction X, it can also push the first stage 21 to drive the microfluidic chip assembly 2000 toward the base 11, so that the droplet outlet 2014 of the microfluidic chip assembly 2000 extends into the corresponding carrier tube 3000.

[0073] For example, please see Figure 8 and Figure 9 , Figure 8 yes Figure 1 The diagram shows a partial structure of the droplet generation device 1000, the microfluidic chip assembly 2000, and the carrier tube 3000 in some other embodiments. Figure 9 yes Figure 8 The diagram shows the structure of the droplet generation device 1000 in another working state.

[0074] The droplet generation device 1000 also includes a linkage component 500, which connects the pressurizing component 300 and the first stage 21. When the pressurizing component 300 moves toward the first stage 21 along the second direction X, the linkage component 500 is used to make the first stage 21 move toward the second stage 22.

[0075] For example, the linkage assembly 500 may include a connecting rod 51, the middle portion 512 of which is rotatably connected to the stop member 212. Before the pressurizing assembly 300 moves towards the first platform 21 along the second direction X, the first end 511 of the connecting rod 51 abuts against the side of the first platform 21 facing the base 11, and the second end 513 of the connecting rod 51 abuts against the linkage block 352 fixed on the bearing seat 35 facing the base 11. At this time, the second end 513 of the connecting rod 51 disengages from the linkage block 352, and the pressure from the linkage block 352 on the second end 513 of the connecting rod 51 is removed. The connecting rod 51 can rotate counterclockwise around the middle part 512 of the connecting rod 51, so that the first end 511 of the connecting rod 51 moves toward the base 11. The first platform 21 also moves toward the base 11 under the action of gravity, so as to realize that the droplet outlet 2014 of the microfluidic chip assembly 2000 extends into the corresponding carrier tube 3000.

[0076] After pressurization is completed, as the pressurizing component 300 moves along the second direction X toward the side away from the first platform 21, the linkage block 352 moves along the second direction X toward the side away from the first platform 21 with the support seat 35 until the linkage block 352 moves to the second end 513 of the connecting rod 51. Thereafter, the second end 513 of the connecting rod 51 is subjected to downward pressure from the linkage block 352, causing the connecting rod 51 to rotate clockwise around its middle portion 512 as the rotation center. The first end 511 of the connecting rod 51 moves toward the side away from the base 11, pushing upward against the first platform 21 as it moves along the first direction Z toward the side away from the base 11, thus resetting the first platform 21. In other embodiments, the linkage component 500 may have other structures, and the linkage component 500 may have other connection relationships with the pressurizing component 300 and the first platform 21. This application embodiment does not limit this.

[0077] Please refer to it again. Figure 1 For example, the droplet generation device 1000 may include a sample loading platform 600, which can be used to place the microfluidic chip assembly 2000 and / or carrier tube 3000 transferred from the external space of the housing 100 to the droplet generation device 1000. In some examples, a transfer window 121 may be provided on the side wall 12, which connects the sample loading platform 600 and the external space of the housing 100 to transfer the microfluidic chip assembly 2000 from the external space of the housing 100 into the sample loading platform 600 through the transfer window 121. The number of transfer windows 121 may be one, two, three or more, and different transfer windows 121 may be provided on the same side wall 12 of the housing 100 or on different side walls 12 of the housing 100 to realize different transfer processes between the droplet generation device 1000 and the external space of the housing 100.

[0078] In some examples, the droplet generation device 1000 may include a drawer 61 mounted on a first transfer window 1211 (hereinafter referred to as the first transfer window 1211 for distinction), and the drawer 61 is slidably connected to the base 11 of the housing 100, and is retractable relative to the first transfer window 1211 on the side wall 12 of the housing 100. In this case, a sample loading platform 600 may be formed inside the drawer 61; in other words, the sample loading platform 600 may be exposed or retracted relative to the first transfer window 1211 as the drawer 61 moves. When manual sample loading is required, drawer 61 can be pulled out so that the sample loading platform 600 is exposed relative to the housing 100, making it easy for the operator to place the microfluidic chip assembly 2000 and / or carrier tube 3000 into the sample loading platform 600; pushing drawer 61 in can drive the microfluidic chip assembly 2000 and carrier tube 3000 into the internal space of the housing 100 of the droplet generation device 1000. By setting drawer 61, the difficulty of sample loading for the operator is effectively reduced.

[0079] For example, at the second transfer window (let's call this transfer window 121 the second transfer window for distinction), Figure 1 (not shown in the image) may be equipped with a transfer track ( Figure 1 (Not shown in the image) The transfer track can connect the droplet generation device 1000 to other devices (e.g., a sample pretreatment device 4000 for processing the microfluidic chip assembly 2000, such as a system builder, or an amplification analysis device for amplifying the droplets). By setting the transfer track at the second transfer window 121, the items to be transferred (e.g., the microfluidic chip assembly 2000 and / or the carrier tube 3000) can be moved directly from one device to another via the transfer track, reducing manual intervention and improving the automation level of transfer within the droplet generation device 1000. Specifically, when the transfer track is used to connect the sample pretreatment device 4000 and the droplet generation device 1000, the sample loading platform 600 can be formed on this portion of the transfer track extending into the droplet generation device 1000. In this embodiment, two transfer methods at the transfer window 121 are exemplarily described. In other embodiments, other transfer methods may also be used, and this embodiment does not limit these methods.

[0080] In some embodiments, the droplet generation device 1000 further includes a scheduling component 700 disposed within the housing 100. The scheduling component 700 is used to realize the transfer process of the microfluidic chip component 2000 and / or carrier tube 3000 within the housing 100 of the droplet generation device 1000. For example, when the microfluidic chip assembly 2000 and / or the carrier tube 3000 are stored in the sample loading platform 600, the control unit can control the scheduling component 700 to transfer the microfluidic chip assembly 2000 of the sample loading platform 600 to the first stage 21, and / or, the control unit can control the scheduling component 700 to transfer the carrier tube 3000 of the sample loading platform 600 to the second stage 22; as another example, after the pressurization component 300 completes the ventilation and pressurization process, the control unit can control the scheduling component 700 to remove the microfluidic chip assembly 2000 from the first stage 21, and / or, the control unit can also control the scheduling component 700 to remove the carrier tube 3000 from the second stage 22.

[0081] By setting the scheduling component 700 to transfer the microfluidic chip assembly 2000 and / or the carrier tube 3000, the manual operation steps in the detection process are reduced, which can effectively improve the transfer efficiency and automation level of the droplet generation device 1000. It is understood that, in this embodiment, by setting the pop-out component 34, the difficulty of the scheduling component 700 transferring the microfluidic chip assembly 2000 is reduced, avoiding the need for manual fixing of the microfluidic chip assembly 2000 when it is transferred to the first stage 21, or manual release of the fixing process when it is transferred out of the first stage 21. This results in a high degree of automation within the droplet generation device 1000.

[0082] Please refer to the following: Figure 1 and Figure 10 , Figure 10 This is a simplified structural diagram of a detection system 1 provided in an embodiment of this application. The droplet generation device 1000 described above can be applied to this detection system 1, or to other detection systems 1; this embodiment of the application does not limit its application in this regard.

[0083] In some embodiments, the detection system 1 may further include the aforementioned microfluidic chip assembly 2000, sample pretreatment device 4000, and first transfer track 4001 (the transfer track here is referred to as the first transfer track 4001 for distinction). The sample pretreatment device 4000 is used to process the microfluidic chip assembly 2000. The droplet generating device 1000 may have a second transfer window 1212 on the side near the sample pretreatment device 4000. The first transfer track 4001 passes through the second transfer window 1212 and connects the droplet generating device 1000 and the sample pretreatment device 4000. The first transfer track 4001 can transport the microfluidic chip assembly 2000 from the sample pretreatment device 4000 to the sample loading platform 600 of the droplet generating device 1000. After that, the control unit can control the scheduling component 700 to remove the microfluidic chip assembly 2000 from the first transfer track 4001 and place it on the first stage 21. In some other embodiments, the detection system 1 may not include the sample pretreatment device 4000, and this application embodiment does not limit this.

[0084] The above are merely some embodiments and implementation methods of this application. The scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A droplet generation apparatus, characterized by, The device comprises a first carrier for placing a microfluidic chip assembly, a pressurizing assembly for pressurizing the microfluidic chip assembly placed on the first carrier to generate droplets, a pop-up member and a sealing member; The pressurizing assembly comprises a pressurizing member and a driving component, the pressurizing member is connected to an output end of the driving component, the driving component drives the pressurizing member to move between a first position and a second position, the pressurizing member is closer to the first carrier in the second position than in the first position, and the sealing member abuts between the microfluidic chip assembly and the pressurizing member in the second position; The pop-up member is connected to the pressurizing member and / or the microfluidic chip assembly, and when the pressurizing member moves from the second position to the first position, the pop-up member pushes one of the microfluidic chip assembly and the pressurizing member to separate them.

2. The droplet generation device of claim 1, wherein, The pop-up member has elastic deformation capability.

3. The droplet generation device of claim 2, wherein, In the second position, the pop-up member abuts between the microfluidic chip assembly and the pressurizing member, and the pop-up member is spaced apart from the sealing member.

4. Droplet generation apparatus according to claim 2 or 3, characterised in that, The pop-up member and the sealing member are both fixed to a side of the pressurizing member facing the first carrier, and the pop-up member protrudes relative to the sealing member when the pressurizing member is in the first position.

5. The droplet generation device of claim 1, wherein, The pop-up member comprises a first magnetic member and a second magnetic member, the first magnetic member is fixed to the pressurizing member, and the second magnetic member is fixed to the microfluidic chip assembly; When the pressurizing member moves from the second position to the first position, repulsion between the first magnetic member and the second magnetic member is used to push the microfluidic chip assembly and the pressurizing member apart.

6. The droplet generation device of any one of claims 1 to 3, wherein, The droplet generation device further comprises a base and a second carrier for placing at least one carrier tube for collecting droplets generated by the microfluidic chip assembly; the first carrier and the second carrier are separately arranged and movably connected to the base; When the pressurizing member moves towards the first carrier, it drives the first carrier to move towards a side of the second carrier, so that the droplet outlet of the microfluidic chip assembly extends into the corresponding carrier tube.

7. The droplet generation device of claim 6, wherein, The droplet generation device further comprises a first horizontal moving component, the first horizontal moving component comprises a first horizontal moving motor and a first horizontal moving member, the pressurizing member is connected to the first horizontal moving member, and the first horizontal moving motor drives the first horizontal moving member to move and drive the pressurizing member to move towards the first carrier.

8. The droplet generation device of any one of claims 1 to 3, wherein, The droplet generation device comprises a sample loading platform and a scheduling assembly, the scheduling assembly is used to transfer the microfluidic chip assembly from the sample loading platform to the first carrier and / or move the microfluidic chip assembly away from the first carrier.

9. A detection system characterized by, The device comprises a microfluidic chip assembly and the droplet generation device according to any one of claims 1-8, and the microfluidic chip assembly is placed on the first carrier.

10. The detection system of claim 9, wherein, The microfluidic chip assembly comprises a microfluidic chip and a connecting frame, the microfluidic chip is fixed to the connecting frame, and the pop-up member is arranged corresponding to the connecting frame in the second position.

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