Nucleic acid detection card box and system

Abstract

The utility model relates to the technical field of in-vitro medical diagnosis, and discloses a nucleic acid detection card box and system.The card box is provided with a sample inlet, and a sample cavity, a magnetic bead cavity and a processing cavity are formed in the card box; the sample cavity is communicated with the sample inlet; the magnetic bead cavity is arranged on one side of the sample cavity along the horizontal direction, and one side of the top end of the magnetic bead cavity is communicated with the bottom end of the sample cavity through a siphon flow channel; magnetic beads are accommodated in the magnetic bead cavities; the bottom of the magnetic bead cavity communicates with the processing cavity through a liquid inlet flow channel which bends and extends upwards, and the communicating position of the liquid inlet flow channel and the processing cavity is higher than the communicating position of the siphon flow channel and the magnetic bead cavity. In this way, the sample liquid can fully remelt the magnetic beads, and the accuracy and reliability of the detection result are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in vitro medical diagnosis, in particular to a nucleic acid detection cartridge and system. BACKGROUND

[0002] The nucleic acid detection cartridge is an important tool for nucleic acid detection. The cartridge adsorbs and condenses the nucleic acid produced after the input sample liquid is lysed by the magnetic beads (i.e. lyophilized beads) pre-contained therein, then filters and removes other impurities in the sample liquid, and finally elutes the nucleic acid from the magnetic beads for subsequent steps such as PCR (polymerase chain reaction, also known as in vitro DNA amplification technology) and optical detection.

[0003] The existing nucleic acid detection cartridge generally has a magnetic bead chamber arranged directly below the sample chamber. The sample chamber and the magnetic bead chamber are connected by a vertical flow channel. The bottom of the magnetic bead chamber is connected to the processing chamber by a flow channel. The magnetic beads are pre-contained in the magnetic bead chamber. After the sample liquid is injected into the sample chamber, it will flow directly into the flow channel at the bottom of the magnetic bead chamber under the action of gravity. Since the cross-sectional area of the magnetic bead chamber is larger than that of the flow channel, the magnetic beads near the edge of the magnetic bead chamber may not be mixed with the sample liquid, resulting in insufficient thawing of the magnetic beads by the sample liquid, and thus affecting the detection result. CONTENT OF THE UTILITY MODEL

[0004] In view of the above problems, the present application provides a nucleic acid detection cartridge and system, which can fully thaw the magnetic beads with the sample liquid, and improve the accuracy and reliability of the detection result.

[0005] According to an aspect of the present application, a nucleic acid detection cartridge is provided. The cartridge is provided with a sample inlet. The inside of the cartridge is provided with a sample chamber, a magnetic bead chamber and a processing chamber. The sample chamber is connected to the sample inlet. The magnetic bead chamber is arranged on one side of the sample chamber along the horizontal direction, and the top end of the magnetic bead chamber is connected to the bottom end of the sample chamber by a siphon flow channel. The magnetic bead chamber contains magnetic beads. The bottom of the magnetic bead chamber is connected to the processing chamber by an upwardly curved liquid inlet flow channel, and the connection position of the liquid inlet flow channel and the processing chamber is higher than the connection position of the siphon flow channel and the magnetic bead chamber.

[0006] In an optional manner, the liquid inlet flow channel has a serpentine extension.

[0007] In an optional manner, the input end of the serpentine extension is higher than the connection position of the siphon flow channel and the magnetic bead chamber.

[0008] In an optional manner, the liquid inlet flow channel further has a resistance chamber extending in the vertical direction and having an increasing cross-sectional area. The resistance chamber is located between the magnetic bead chamber and the serpentine extension.

[0009] In an alternative, the top end of the choke cavity is higher than or equal to the position where the siphon flow channel communicates with the magnetic bead cavity.

[0010] In an alternative, the bottom of the magnetic bead cavity is provided with a guide slope, which is inclined towards the input end of the liquid inlet flow channel.

[0011] In an alternative, the cartridge is provided with air holes, which include first air holes communicating with the sample cavity and / or second air holes communicating with the processing cavity.

[0012] In an alternative, the sample liquid is injected into the sample cavity at a height lower than or equal to the height of the position where the siphon flow channel communicates with the magnetic bead cavity, so that the sample liquid is retained in the sample cavity and the siphon flow channel; the air holes are used to introduce pressure to drive the sample liquid into the magnetic bead cavity to mix with the magnetic beads to form a mixed fluid; the air holes are also used to introduce pressure to drive the mixed fluid into the processing cavity.

[0013] In an alternative, the liquid inlet flow channel has a serpentine extension, and the air holes are used to alternately introduce positive pressure and negative pressure to drive the mixed fluid to reciprocally flow in the magnetic bead cavity and the serpentine extension.

[0014] According to another aspect of the embodiments of the present application, a nucleic acid detection system is provided, which includes a detection device and the nucleic acid detection cartridge of any of the above, and the detection device is used to fix and operate the nucleic acid detection cartridge to perform nucleic acid detection.

[0015] In the nucleic acid detection cartridge provided by the embodiments of the present application, the magnetic bead cavity is arranged on one side of the sample cavity in the horizontal direction, and the top end of the magnetic bead cavity is communicated with the bottom end of the sample cavity through the siphon flow channel, so that the sample liquid injected into the sample cavity does not directly flow through the magnetic bead cavity, avoiding the problem of insufficient mixing of the magnetic beads. In addition, the output end of the siphon flow channel is communicated with one side of the top end of the magnetic bead cavity, which enables the sample liquid to more completely cover the internal space of the magnetic bead cavity in the horizontal direction during the process of pumping the sample liquid from the sample cavity into the magnetic bead cavity and flowing from top to bottom in the magnetic bead cavity, so as to sufficiently mix the magnetic beads inside. After the sample liquid flows out of the sample cavity, since the bottom of the magnetic bead cavity is communicated with the processing cavity through the upwardly curved liquid inlet flow channel, and the communication position of the liquid inlet flow channel and the processing cavity is higher than the communication position of the siphon flow channel and the magnetic bead cavity, the sample liquid is retained in the magnetic bead cavity and the liquid inlet flow channel, so as to provide sufficient contact and remelting time for the magnetic beads and the sample liquid, and to ensure sufficient mixing and remelting of the magnetic beads, thereby providing good guarantee for the accuracy and reliability of the detection result.

[0016] The above description is only an overview of the technical solution of this application. In order to better understand the technical means of this application and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this application more obvious and understandable, the following are specific embodiments of this application. Attached Figure Description

[0017] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0018] Figure 1 A perspective view of one side of the nucleic acid detection card box provided in an embodiment of this utility model;

[0019] Figure 2 A perspective view of the nucleic acid testing card box from another side, provided in an embodiment of this utility model;

[0020] Figure 3 A perspective view of the front of the nucleic acid testing card box provided in an embodiment of this utility model;

[0021] Figure 4 A diagram showing the liquid level of the nucleic acid detection cartridge provided in this embodiment of the invention after sample liquid has been injected into the sample chamber;

[0022] Figure 5 This is a perspective view of the front of another embodiment of the nucleic acid detection card box of this utility model;

[0023] Figure 6 A diagram showing the liquid level of the nucleic acid detection cartridge provided in this embodiment of the invention after the sample solution enters the magnetic bead cavity;

[0024] Figure 7 An exploded view of the nucleic acid testing card box provided in this embodiment of the utility model;

[0025] Figure 8 A flowchart of the nucleic acid detection method provided in this embodiment of the utility model;

[0026] Figure 9 This is a diagram showing the liquid surface state of the mixed fluid in the nucleic acid detection cartridge after it enters the processing chamber, as provided in an embodiment of this utility model.

[0027] Figure 10a and Figure 10b These are diagrams showing two liquid surface states in the nucleic acid detection cartridge provided in this embodiment of the invention during the reciprocating flow of the mixed fluid in the magnetic bead cavity and the serpentine extension section.

[0028] The reference numerals in the detailed embodiments are as follows:

[0029] 100. Card holder; 101. Card holder body; 102. Film;

[0030] 110. Sample inlet; 120. Sample chamber; 130. Magnetic bead chamber; 131. Guide ramp; 140. Processing chamber; 150. Siphon channel; 160. Liquid inlet channel; 161. Serpentine extension section; 162. Flow retardation chamber; 171. First vent; 172. Second vent;

[0031] 200, Sample solution; 210, Mixed fluid. Detailed Implementation

[0032] The embodiments of the technical solution of this application will now be described in detail with reference to the accompanying drawings. These embodiments are only used to more clearly illustrate the technical solution of this application and are therefore merely examples, and should not be used to limit the scope of protection of this application.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms “comprising” and “having”, and any variations thereof, in the specification, claims, and foregoing description of the drawings are intended to cover non-exclusive inclusion.

[0034] In the description of the embodiments of this application, technical terms such as "first" and "second" are used only to distinguish different objects and should not be construed as indicating or implying relative importance or implicitly specifying the number, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, "multiple" means two or more, unless otherwise explicitly defined.

[0035] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0036] In the description of the embodiments in this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent three cases: A exists, A and B exist simultaneously, and B exists. In addition, the character " / " in this document generally indicates that the related objects before and after it have an "or" relationship.

[0037] In the description of the embodiments of this application, the term "multiple" refers to two or more (including two), similarly, "multiple sets" refers to two or more (including two sets), and "multiple pieces" refers to two or more (including two pieces).

[0038] In the description of the embodiments of this application, the technical terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this application and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.

[0039] In the description of the embodiments of this application, unless otherwise expressly specified and limited, technical terms such as "installation," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. For those skilled in the art, the specific meaning of the above terms in the embodiments of this application can be understood according to the specific circumstances.

[0040] To ensure that the input sample solution fully remelts the magnetic beads within the magnetic bead cavity, this application has made corresponding improvements to the relative position and communication between the magnetic bead cavity and the sample cavity in the nucleic acid detection cartridge. Specifically, the magnetic bead cavity is positioned on one side of the sample cavity along the horizontal direction, and the top of the magnetic bead cavity is connected to the bottom of the sample cavity via a siphon channel. This ensures that the injected sample solution first stays in the siphon channel and the sample cavity without directly entering the magnetic bead cavity, thus preventing the sample solution from flowing directly through the magnetic bead cavity and failing to remelt all the magnetic beads inside. After the sample solution is injected, pressure can be applied to the sample cavity or negative pressure can be introduced into the processing cavity connected to the output end of the magnetic bead cavity to allow the sample solution to begin flowing into the magnetic bead cavity through the siphon channel under the siphon effect. It is connected to one side of the top of the magnetic bead cavity. Therefore, the sample liquid that just flows into the magnetic bead cavity from the output end of the siphon channel has an initial velocity in the horizontal direction. After the sample liquid enters the magnetic bead cavity, it has an initial velocity in the horizontal direction, and at the same time, it is affected by gravity in the vertical direction and will move along a parabolic path. At the same time, during the movement, the collision between the sample liquid and the inner wall of the magnetic bead cavity, as well as the collision between the sample liquids, can make the sample liquid reach the inner wall around the magnetic bead cavity better, so as to cover the entire cross-section of the magnetic bead cavity. In addition, the sample liquid attached to the inner wall of the magnetic bead cavity will also flow downward along the inner wall of the magnetic bead cavity under its own gravity. The above-mentioned movement of the sample liquid in the magnetic bead cavity can make the sample liquid better remelt the magnetic beads located on the surrounding side walls of the magnetic bead cavity, so as to achieve the purpose of fully remelting the magnetic beads in the magnetic bead cavity.

[0041] In addition, the bottom of the magnetic bead cavity is connected to the processing chamber via an upwardly curved inlet channel. The connection point between the inlet channel and the processing chamber is higher than the connection point between the siphon channel and the magnetic bead cavity. This ensures that after the sample liquid flows into the magnetic bead cavity, it will not continue to flow under gravity and completely enter the inlet channel after the magnetic bead cavity. Instead, when the pressure at both ends of the sample liquid is balanced, one part of the sample liquid is located in the inlet channel, and the other part is located in the magnetic bead cavity. Correspondingly, as the sample liquid flows and is held, some magnetic beads will enter the inlet channel, while other magnetic beads will remain in the magnetic bead cavity. This allows the magnetic beads to fully contact and remelt with the sample liquid, ensuring the accuracy and reliability of the test results.

[0042] According to one aspect of the embodiments of this application, a nucleic acid detection kit is provided; please refer to [the specific details]. Figures 1 to 3 , Figure 1 and Figure 2 The three-dimensional structure of the card case is shown from both sides. Figure 3 This shows the perspective structure of the card box from one side. For example... Figures 1 to 3As shown, the cartridge 100 has a sample inlet 110. The interior of the cartridge 100 is provided with a sample chamber 120, a magnetic bead chamber 130 and a processing chamber 140. The sample chamber 120 is connected to the sample inlet 110. The magnetic bead chamber 130 is located on one side of the sample chamber 120 in the horizontal direction. The top side of the magnetic bead chamber 130 is connected to the bottom side of the sample chamber 120 through a siphon channel 150. The magnetic bead chamber 130 contains magnetic beads. The bottom of the magnetic bead chamber 130 is connected to the processing chamber 140 through an upwardly curved liquid inlet channel 160. The connection position A between the liquid inlet channel 160 and the processing chamber 140 is higher than the connection position B between the siphon channel 150 and the magnetic bead chamber 130.

[0043] Specifically, the card box 100 can form a cavity and flow channel inside by means of integral molding, two main parts correspondingly slotted and interlocked, or surface slotting and attaching a cover plate.

[0044] Based on the above structural design, during nucleic acid testing, the sample solution is first injected into the sample cavity 120 through the injection port 110. Since the magnetic bead cavity 130 is located on one side of the sample cavity 120 along the horizontal direction, and the top of the magnetic bead cavity 130 is connected to the bottom of the sample cavity 120 via a siphon channel 150, to ensure that the sample solution injected into the sample cavity 120 remains within the siphon channel 150 and at the bottom of the sample cavity 120, and to prevent the sample solution from prematurely entering the magnetic bead cavity 130 and affecting the subsequent rehydration effect of the magnetic beads, the liquid level of the injected sample solution should preferably not exceed position B where the siphon channel 150 connects to the magnetic bead cavity 130. Specifically, after the sample solution is completely injected, it can appear as follows: Figure 4 As shown in the figure, the area indicated by the dense dashed lines represents the sample liquid 200. It can be seen from the figure that the liquid level of the sample liquid 200 does not exceed the connection point B between the siphon channel 150 and the magnetic bead cavity 130, and the sample liquid 200 will not enter the magnetic bead cavity 130 in advance.

[0045] After the sample solution is injected, the sample solution can be driven into the magnetic bead cavity 130 through the siphon channel 150 by pressurizing the sample cavity 120, or the sample solution can be made to flow from the sample cavity 120 into the magnetic bead cavity 130 under the siphon effect by introducing negative pressure in the processing cavity 140. Specifically, as shown... Figures 1 to 4 As shown, the cartridge 100 can be provided with an air vent communicating with the sample chamber 120. The air vent may include a first air vent 171. After the first air vent 171 is connected to a gas pipeline and positive pressure is introduced, the sample liquid is driven. It should be noted here that... Figure 3In the specific embodiment shown, each cavity and flow channel is implemented by slotting the cartridge 100 and covering it with a thin film. The solid line in the figure represents the cavity and flow channel structure shown on the front, and the dashed line represents the cavity and flow channel structure on the back that is not shown. At the junction of the solid and dashed lines, the front flow channel and the back flow channel are interconnected through a through hole penetrating the cartridge 100. The flow direction of the gas after the first air hole 171 is connected to positive pressure is as follows. Figure 4 As shown by the dashed arrow. Figure 5 and Figure 6 In another embodiment shown, as illustrated in the perspective view of the front of the cartridge, the vent may also include a second vent 172 communicating with the processing chamber 140. By applying negative pressure to the second vent 172, the sample liquid can also be drawn into the magnetic bead chamber 130. The gas flow direction after applying negative pressure to the second vent 172 is as follows: Figure 6 As indicated by the dashed arrow. Of course, in some other embodiments, the pores may also include both a first pore 171 and a second pore 172, thereby achieving efficient driving of the sample liquid by introducing positive pressure in the first pore 171 and negative pressure in the second pore 172.

[0046] In some embodiments, the sample chamber 120 is used to inject sample liquid, and the injection height of the sample liquid in the sample chamber 120 is lower than or equal to the end of the siphon channel 150 that communicates with the magnetic bead cavity 130, that is, after the sample liquid is injected into the sample chamber 120, it is maintained at a height of approximately [missing information]. Figure 4 The state is shown. The vents are used to introduce pressure to drive the sample liquid into the magnetic bead cavity 130 to mix with the magnetic beads and form a mixed fluid. Specifically, positive pressure can be introduced through the first vent 171, or negative pressure can be introduced through the second vent 172. The vents are also used to introduce pressure to drive the mixed fluid into the processing chamber 140.

[0047] Understandably, since the output end of the siphon channel 150 is connected to one side of the top of the magnetic bead cavity 130, the sample liquid entering the magnetic bead cavity 130 from the output end of the siphon channel 150 has a horizontal velocity. This allows a portion of the sample liquid to reach the inner wall around the magnetic bead cavity 130 and flow downward along the inner wall. As the sample liquid enters the magnetic bead cavity 130 and flows downward, it can better fill the entire cross-section of the magnetic bead cavity 130, thereby mixing with all the magnetic beads in the magnetic bead cavity 130 and remelting them.

[0048] Considering that the sample solution can better remelt the magnetic beads after a longer contact time with them, this embodiment of the application further connects the bottom of the magnetic bead cavity 130 to the processing cavity 140 through an upwardly curved liquid inlet channel 160. This ensures that after the sample solution has completely flowed out of the sample cavity 120, the sample solution and the magnetic beads mixed in the sample solution can remain in the magnetic bead cavity 130 and the liquid inlet channel 160 without external driving pressure, so that the magnetic beads can be fully mixed and contacted with the sample solution before remelting.

[0049] Subsequently, the driving pressure can be reintroduced to allow the mixed fluid formed by the sample solution and magnetic beads to enter the processing chamber 140 through the inlet channel 160, and the nucleic acid detection process can be completed through subsequent processing of the mixed fluid.

[0050] In summary, in the nucleic acid detection cartridge provided in this application embodiment, by placing the magnetic bead cavity 130 on one side of the sample cavity 120 along the horizontal direction, and connecting the top of the magnetic bead cavity 130 to the bottom of the sample cavity 120 through the siphon channel 150, the sample liquid injected into the sample cavity 120 does not flow directly through the magnetic bead cavity 130, thus avoiding the problem of insufficient mixing of the magnetic beads. Furthermore, the output end of the siphon channel 150 is connected to one side of the top of the magnetic bead cavity 130. This allows the sample liquid to more completely cover the internal space of the magnetic bead cavity 130 in the horizontal direction during the process of pumping the sample liquid from the sample cavity 120 into the magnetic bead cavity 130 and flowing from top to bottom within the magnetic bead cavity 130, thereby ensuring thorough mixing of the internal magnetic beads. After all the sample solution flows out of the sample chamber 120, the bottom of the magnetic bead chamber 130 is connected to the processing chamber 140 through the upwardly curved liquid inlet channel 160. The connection position between the liquid inlet channel 160 and the processing chamber 140 is higher than the connection position between the siphon channel 150 and the magnetic bead chamber 130. Therefore, the sample solution will remain in the magnetic bead chamber 130 and the liquid inlet channel 160, providing sufficient contact and remelting time for the magnetic beads and the sample solution. This ensures that the magnetic beads are fully mixed and remelted, thus providing a good guarantee for the accuracy and reliability of the test results.

[0051] To further ensure that the magnetic beads are mixed evenly in the sample solution, thus facilitating their refusion, such as... Figure 3 As shown, the inlet channel 160 may have a serpentine extension section 161. After the sample liquid reaches the magnetic bead cavity 130 and mixes with the magnetic beads therein to form a mixed fluid, the mixed fluid will flow through the serpentine extension section 161 during the process of pumping the mixed fluid into the processing cavity 140. On the one hand, this can increase the flow distance of the mixed fluid, so that the magnetic beads can be fully mixed with the sample liquid during the flow. On the other hand, the resistance effect of the bend of the serpentine extension section 161 on the sample liquid and the magnetic beads can also make the magnetic beads and the sample liquid mix more fully, thus ensuring the remelting of the magnetic beads.

[0052] Furthermore, based on the serpentine extension section 161, before the mixed fluid is input into the processing chamber 140, positive and negative pressures can be alternately introduced through the vents to drive the mixed fluid to reciprocate within the magnetic bead chamber 130 and the serpentine extension section 161, thereby achieving more thorough mixing. Further, in the embodiment where a flow-blocking cavity 162 is provided in the inlet channel 160, the mixed fluid is driven to reciprocate within the magnetic bead chamber 130, the flow-blocking cavity 162, and the serpentine extension section 161.

[0053] To prevent the sample solution from prematurely entering the serpentine extension 161 on the inlet channel 160 after being injected into the magnetic bead cavity 130, thus affecting the mixing effect between the sample solution and the magnetic beads, such as... Figure 3 As shown, the input end C of the liquid inlet channel 160 is higher than the connection position B between the siphon channel 150 and the magnetic bead cavity 130. This setting can ensure that after a certain amount of sample liquid is injected into the magnetic bead cavity 130 and mixed with the magnetic beads, the sample liquid can be better retained in the liquid inlet channel 160 before the input end of the serpentine extension section 161 and the magnetic bead cavity 130, so as to ensure that the magnetic beads can be fully mixed and soaked in the sample liquid for remelting.

[0054] Based on this, in order to better prevent the sample solution from prematurely entering the serpentine extension 161, such as Figure 3 As shown, the liquid inlet channel 160 may also have a flow-blocking cavity 162 that extends vertically and has an increased cross-sectional area, the flow-blocking cavity 162 being located between the magnetic bead cavity 130 and the serpentine extension 161.

[0055] Since the flow-blocking cavity 162 extends vertically, after the sample liquid injected into the magnetic bead cavity 130 mixes with the magnetic beads to form a mixed fluid, the height of the front end of the mixed fluid gradually increases as it reaches the flow-blocking cavity 162, and the flow resistance gradually increases. At the same time, the cross-section of the flow-blocking cavity 162 is larger than other positions of the liquid inlet channel 160, which allows the flow-blocking cavity 162 to accommodate more mixed fluid, thereby better preventing the mixed fluid from entering the serpentine extension section 161.

[0056] Furthermore, such as Figure 3 As shown, the output terminal D of the choke cavity 162 can be higher than or equal to the connection position B between the siphon channel 150 and the magnetic bead cavity 130. It should be noted that... Figure 3 In the specific embodiment shown, the output terminal D of the flow-blocking cavity 162 is directly connected to the input terminal C of the serpentine extension section 161. Of course, in some other embodiments, the output terminal D of the flow-blocking cavity 162 can also be connected to the input terminal C of the serpentine extension section 161 through a flow channel.

[0057] Since the output end D of the flow-restricting cavity 162 is higher than or equal to the connection position B between the siphon channel 150 and the magnetic bead cavity 130, the liquid level of the mixed fluid in the flow-restricting cavity 162 and the liquid level in the magnetic bead cavity 130 are the same without external driving pressure. This allows the mixed fluid 210 to flow as... Figure 6 The magnetic beads are completely contained within the magnetic bead cavity 130, the flow-blocking cavity 162, and the liquid inlet channel 160 between the magnetic bead cavity 130 and the flow-blocking cavity 162, ensuring that the magnetic beads can be fully mixed and remelted with the sample solution.

[0058] In order to allow the magnetic beads in the magnetic bead cavity 130 to mix better with the sample solution and flow with the sample solution, such as Figure 7 As shown in the exploded structure, a guide ramp 131 is provided at the bottom of the magnetic bead cavity 130, and the guide ramp 131 is inclined towards the input end E of the liquid inlet channel 160. It should be noted that in... Figure 7 In the specific embodiment shown, the card holder 100 includes a card holder body 101 and a film 102. The card holder body 101 has a slot and the film 102 covers the slot opening to form a magnetic bead cavity 130 inside the slot.

[0059] By providing a guide ramp at the bottom of the magnetic bead cavity 130 that slopes towards the input end E of the liquid inlet channel 160, the magnetic beads can flow into the liquid inlet channel 160 along the guide ramp and remelt with the sample liquid, preventing dead corners at the bottom of the magnetic bead cavity 130 from causing some magnetic beads to fail to enter the liquid inlet channel 160.

[0060] According to another aspect of the embodiments of this application, a nucleic acid detection system is also provided. The system includes a detection device and a cartridge 100 as described in any of the above embodiments. The detection device is used to fix and operate the cartridge 100 to perform nucleic acid detection.

[0061] According to another aspect of the embodiments of this application, a nucleic acid detection method is also provided. This method is applied to the cartridge 100 provided with a first vent 171 and / or a second vent 172 in the above embodiments. Please refer to [link to relevant documentation] for details. Figure 8 The diagram illustrates the workflow of a nucleic acid testing method, which includes the following steps:

[0062] Step 310: Inject the sample solution into the sample chamber 120 through the injection port 110, ensuring that the injection height of the sample solution in the sample chamber 120 is lower than or equal to the height of the end of the siphon channel 150 that connects to the magnetic bead chamber 130, so that the sample solution is held within the magnetic bead chamber 130 and the siphon channel 150. Specifically, it can be presented as follows: Figure 4 The state shown.

[0063] Step 330: Positive pressure is introduced into the first pore 171 to drive the sample liquid into the magnetic bead cavity 130 and mix it with the magnetic beads to form a mixed fluid. Specifically, it can be presented as follows:Figure 6 The state shown.

[0064] Step 350: Positive pressure is introduced again through the first vent 171 to drive the mixed fluid into the processing chamber 140. Specifically, it can be presented as follows: Figure 9 The state shown.

[0065] The mixed fluid can then be further processed in the processing chamber 140 to achieve nucleic acid detection of the sample solution.

[0066] It should be noted that, for steps 330 and 350 above, besides using a first vent 171 connected to the sample chamber 120 and introducing positive pressure through the first vent 171 to drive the sample liquid into the magnetic bead chamber 130 and the processing chamber 140, other methods can also be used as follows: Figure 6 The second vent 172 shown is connected to the processing chamber 140, and negative pressure is introduced through the second vent 172 to drive the sample liquid. Of course, the first vent 171 and the second vent 172 can also be set at the same time, with positive pressure introduced through the first vent 171 and negative pressure introduced through the second vent 172, so as to jointly drive the sample liquid from the sample chamber 120 into the magnetic bead chamber 130 and from the magnetic bead chamber 130 into the processing chamber 140.

[0067] The nucleic acid detection method provided in this application embodiment is applied to the cartridge 100 provided in the above embodiment. After adding the sample solution, the injection height of the sample solution in the sample cavity 120 is lower than or equal to the height of the end of the siphon channel 150 that connects to the magnetic bead cavity 130. This ensures that the sample solution injected into the sample cavity 120 does not flow directly into the magnetic bead cavity 130, avoiding the problem of insufficient mixing of the magnetic beads. Then, by introducing positive pressure through the first vent 171, or negative pressure through the second vent 172, or by introducing positive pressure through the first vent 171 and negative pressure through the second vent 172 simultaneously, the sample solution is driven into the magnetic bead cavity 130. This ensures that during the process of the sample solution entering the magnetic bead cavity 130 from the sample cavity 120 and flowing from top to bottom in the magnetic bead cavity 130, the sample solution can more completely cover the internal space of the magnetic bead cavity 130 in the horizontal direction, thereby fully mixing the internal magnetic beads. Furthermore, after all the sample solution flows out of the sample chamber 120, the sample solution will remain in the magnetic bead chamber 130 and the liquid inlet channel 160, so as to provide sufficient contact and remelting time for the magnetic beads and the sample solution. This ensures that the magnetic beads are fully mixed and remelted, thus providing a good guarantee for the accuracy and reliability of the test results.

[0068] When this nucleic acid detection method is applied to the scheme where the liquid inlet channel 160 of the nucleic acid detection cartridge provided in the above embodiment has a serpentine extension section 161, after step 330, the nucleic acid detection method may further include the following steps:

[0069] Step 340: Alternating positive and negative pressure is introduced into the first vent 171 to drive the mixed fluid to reciprocate within the magnetic bead cavity 130 and the serpentine extension 161, thereby mixing the sample liquid in the mixed fluid with the magnetic beads. Specifically, the mixed fluid can be... Figure 10a and Figure 10b Switching back and forth between the shown states.

[0070] Similarly, for step 340, in addition to alternately introducing positive and negative pressure in the first vent 171 to drive the reciprocating flow of the mixed fluid, it can also be achieved by alternately introducing negative and positive pressure in the second vent 172. Alternatively, positive and negative pressure can be alternately introduced in both the first vent 171 and the second vent 172, thereby driving the reciprocating flow of the mixed fluid through the suction and thrust formed at both ends.

[0071] Before injecting the mixed fluid into the processing chamber 140, the mixed fluid is driven to flow back and forth in the serpentine extension section 161. On the one hand, this increases the flow distance of the mixed fluid, allowing the magnetic beads to mix fully with the sample liquid during the flow. On the other hand, the resistance effect of the bends in the serpentine extension section 161 on the sample liquid and the magnetic beads also allows the magnetic beads to mix more fully with the sample liquid, ensuring the remelting of the magnetic beads.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and not to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application. In particular, as long as there is no structural conflict, the various technical features mentioned in the various embodiments can be combined in any way.

Claims

1. A nucleic acid testing cartridge, characterized by, The cartridge is provided with a sample inlet, and the cartridge is internally provided with a sample chamber, a magnetic bead chamber and a treatment chamber; The sample chamber is in communication with the sample inlet; The magnetic bead chamber is arranged at one side of the sample chamber along a horizontal direction, and one side of the top end of the magnetic bead chamber is in communication with the bottom end of the sample chamber through a siphon flow channel; The magnetic bead chamber is internally provided with magnetic beads; The bottom of the magnetic bead chamber is in communication with the treatment chamber through a liquid inlet flow channel extending upwardly and bending, and the communication position of the liquid inlet flow channel with the treatment chamber is higher than the communication position of the siphon flow channel with the magnetic bead chamber.

2. The nucleic acid testing cartridge of claim 1, wherein, The liquid inlet flow channel has a serpentine extension section.

3. The nucleic acid testing cartridge of claim 2, wherein, The input end of the serpentine extension section is higher than the communication position of the siphon flow channel with the magnetic bead chamber.

4. The nucleic acid testing cartridge of claim 3, wherein, The liquid inlet flow channel further has a resistance flow chamber extending along a vertical direction and increasing in cross-sectional area, and the resistance flow chamber is located between the magnetic bead chamber and the serpentine extension section.

5. The nucleic acid testing cartridge of claim 4, wherein, The top end of the resistance flow chamber is higher than or equal to the communication position of the siphon flow channel with the magnetic bead chamber.

6. The nucleic acid testing cartridge of claim 1, wherein, The bottom of the magnetic bead chamber is provided with a guide slope, and the guide slope is inclined towards the input end of the liquid inlet flow channel.

7. The nucleic acid testing cartridge of any one of claims 1 or 3-6, wherein, The cartridge is provided with an air hole; The air hole includes a first air hole in communication with the sample chamber and / or a second air hole in communication with the treatment chamber.

8. The nucleic acid testing cartridge of claim 7, wherein, The sample chamber is used for injecting a sample liquid, and the injection height of the sample liquid in the sample chamber is lower than or equal to the height of the end in communication of the siphon flow channel with the magnetic bead chamber, so that the sample liquid is kept in the sample chamber and the siphon flow channel; The air hole is used for introducing pressure to drive the sample liquid into the magnetic bead chamber to mix with the magnetic beads to form a mixed fluid; The air hole is also used for introducing pressure to drive the mixed fluid into the treatment chamber.

9. The nucleic acid testing cartridge of claim 8, wherein, The liquid inlet flow channel has a serpentine extension section, and the air hole is also used for alternately introducing positive pressure and negative pressure to drive the mixed fluid to reciprocally flow in the magnetic bead chamber and the serpentine extension section.

10. A nucleic acid detection system, characterized by, The nucleic acid detection cartridge of any one of claims 1-7 is fixed and operated by a detection device to perform nucleic acid detection.

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