Phage screening and purifying device

Abstract

The utility model relates to the technical field of bacteriophage preparation, in particular to a bacteriophage screening and purifying device which comprises vertical plates, a supporting plate fixedly arranged between the two sets of vertical plates, a communicating pipe vertically and fixedly arranged in the supporting plate in a penetrating mode, a purifying cylinder arranged at the communicating pipe on the supporting plate, and a detachable inner cylinder arranged on the upper side in the purifying cylinder. A secondary filter membrane is arranged at the bottom of the detachable inner cylinder, primary filter membranes are arranged on the upper side of the secondary filter membrane at intervals, a liquid storage tank is connected to the lower side of the connecting seat through threads in a sealed mode, an electric push rod is installed on the fixing plate, a pressing rod capable of sliding up and down is arranged in the top plate in a penetrating mode, and a pressing plate matched with the purification cylinder in specification is fixedly arranged at the lower end of the pressing rod. The pressing rod is driven by the electric push rod to move up and down, and the pressing plate at the lower end of the pressing rod can compress air in the purification cylinder, so that pressure is applied to a phage stock solution in the purification cylinder, the speed of the phage stock solution passing through a filter membrane is increased, the filter time of the phage stock solution is shortened, and the purification efficiency of the phage stock solution is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to bacteriophage preparation technical field, specifically, relate to a bacteriophage screening and purification device. BACKGROUND

[0002] Bacteriophage is a kind of virus that parasitizes in bacteria. They have some characteristics of viruses, such as individual small, no cell structure, only containing single nucleic acid (DNA or RNA) etc. The parasitization of bacteriophage on bacteria has high specificity, that is, a bacteriophage can only parasitize in one or a few bacteria with close genetic relationship. Bacteriophage widely exists in nature, is the most common and widely distributed group among viruses. They have important significance for the growth and variation of bacteria and the prevention and treatment of bacterial diseases.

[0003] Bacteriophage is a kind of virus that can infect bacteria, and has wide application prospect in the fields of medicine, food industry, etc. However, in the process of bacteriophage screening and purification, the bacteriophage stock solution is usually poured on the filter membrane by manual operation, and then the bacteriophage stock solution is filtered through the filter membrane by gravity, to realize the filtration and purification of bacteriophage. The operation is tedious and time-consuming. Therefore, it is particularly important to develop an efficient and convenient bacteriophage screening and purification device. UTILITY MODEL CONTENT

[0004] In view of the existing deficiencies, the utility model provides a bacteriophage screening and purification device, which solves the problems raised in the background art.

[0005] To achieve the above purpose, the technical scheme adopted by the utility model is as follows:

[0006] A bacteriophage screening and purification device, comprising a bottom plate and a vertical plate, the bottom plate is provided with a vertical plate on both sides of the upper end face, a digital display controller is installed on the right vertical plate, a supporting plate is fixed between the two groups of vertical plates, a top plate is fixed on the top of the two groups of vertical plates, a communication pipe is vertically penetrated and fixed in the supporting plate, a purification cylinder is placed on the communication pipe of the supporting plate, a cooling assembly is arranged outside the purification cylinder, the bottom of the purification cylinder is funnel-shaped structure, and a plug-in pipe matched with the communication pipe is fixed at the bottom end, the plug-in pipe and the communication pipe are detachably plugged, a detachable inner cylinder is arranged on the upper side of the inner cylinder, a secondary filter membrane is arranged on the bottom of the detachable inner cylinder, a primary filter membrane is arranged on the upper side of the secondary filter membrane, a connecting seat is fixed on the bottom of the supporting plate, a liquid storage tank is connected to the lower side of the connecting seat through threaded sealing, a horizontal fixing plate is fixed on the rear side of the two groups of vertical plates, an electric push rod is installed on the fixing plate, a pressure rod is vertically arranged in the top plate and can slide up and down, the pressure rod is coaxial with the purification cylinder, a pressure plate matched with the specification of the purification cylinder is fixed on the lower end of the pressure rod, a pressure sensor is embedded in the bottom of the pressure plate, and the upper end of the pressure rod is connected to the output end of the electric push rod through a connecting rod.

[0007] Furthermore, the bottom surface and the ring surface of the pressure plate are covered with a layer of rubber sleeve for sealing.

[0008] Furthermore, the support plate is provided with a positioning groove adapted to the purification cylinder, and the connecting pipe is located at the center of the positioning groove.

[0009] Furthermore, the cooling assembly includes an air inlet pipe, a quick-connect female connector, a connecting hose, a sealing and insulating sleeve, and a quick-connect male connector. The sealing and insulating sleeve has a hollow structure that covers the annular surface of the purification cylinder. A connecting hose is connected to the left side of the upper surface of the sealing and insulating sleeve, and a quick-connect male connector is installed at the end of the connecting hose. An air inlet pipe is installed through the interior of the left vertical plate. A quick-connect female connector that matches the quick-connect male connector is installed on the right end of the air inlet pipe, and its left end is connected to an external cooling device. A pressure relief nozzle for pressure relief is installed on the right side of the upper surface of the sealing and insulating sleeve.

[0010] Furthermore, ear plates are connected to both the left and right sides of the connecting plate, and two sets of guide rods are symmetrically fixed to the upper surface of the top plate. The ear plates are sleeved through the guide rods and slidably connected to them.

[0011] Furthermore, a layer of anti-slip rubber pad is adhered to the bottom of the base plate.

[0012] Furthermore, the primary filter membrane is a coarse filter membrane with a pore size of 0.55 μm, and the secondary filter membrane is a fine filter membrane with a pore size of 0.22 μm.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. This utility model uses an electric push rod to drive the pressure rod to move up and down. The pressure plate at the lower end of the pressure rod can compress the air inside the purification cylinder, thereby applying pressure to the phage stock solution inside the purification cylinder, accelerating the speed at which the phage stock solution passes through the filter membrane, thus shortening the filtration time of the phage stock solution, simplifying the operation process, and improving the filtration and purification efficiency of the phage stock solution.

[0015] 2. The design of the detachable inner cylinder and the secondary and primary filter membranes makes it easy to replace the filter membranes, which can meet the screening and purification needs of different phages. At the same time, the combined use of the secondary and primary filter membranes improves the purification efficiency and can remove impurities more effectively.

[0016] 3. The present invention can introduce cold air to cool the inside of the purification cylinder through the cooling component, so as to keep the inside of the purification cylinder in a low temperature environment and thus maintain the activity of the bacteriophage. Attached Figure Description

[0017] Fig. 1 This is a schematic diagram of the overall structure of this utility model.

[0018] Fig. 2 This is a front view of the present invention.

[0019] Fig. 3 This is a schematic diagram of the rear structure of this utility model.

[0020] Fig. 4 This is a cross-sectional view of the pressure plate in this utility model.

[0021] Fig. 5 This is a partial disassembly diagram of the present invention.

[0022] Fig. 6 This is a cross-sectional view of the purification cylinder in this utility model.

[0023] In the diagram: 1. Connecting plate; 2. Pressure rod; 3. Top plate; 4. Pressure plate; 5. Purification cylinder; 6. Cooling assembly; 61. Air inlet pipe; 62. Quick-connect female connector; 63. Connecting hose; 64. Sealing and insulation sleeve; 65. Quick-connect male connector; 66. Pressure relief nozzle; 7. Support plate; 8. Liquid storage tank; 9. Base plate; 91. Anti-slip pad; 10. Vertical plate; 11. Digital display controller; 12. Connecting seat; 13. Ear plate; 14. Guide rod; 15. Electric push rod; 16. Fixing plate; 17. Rubber sleeve; 18. Pressure sensor; 19. Positioning groove; 20. Connecting pipe; 21. Removable inner cylinder; 22. Primary filter membrane; 23. Secondary filter membrane; 24. Insertion pipe. Detailed Implementation

[0024] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0025] Example:

[0026] like Figs. 1 to 6As shown, a phage screening and purification device includes a base plate 9 and upright plates 10. Upright plates 10 are fixed to both sides of the upper surface of the base plate 9. A digital display controller 11 is installed on the right upright plate 10. A support plate 7 is fixed between the two sets of upright plates 10. A top plate 3 is fixed to the top of the two sets of upright plates 10. A connecting pipe 20 is vertically fixed inside the support plate 7. A purification cylinder 5 is placed at the connecting pipe 20 on the support plate 7. A cooling component 6 is provided on the outside of the purification cylinder 5. The bottom of the purification cylinder 5 has a funnel-shaped structure, and a connector 24 adapted to the connecting pipe 20 is fixed at the bottom end. The connector 24 and the connecting pipe 20 are detachably connected. A detachable inner cylinder 21 is provided on the upper side of the purification cylinder 5. A secondary filter membrane 23 is provided at the bottom of the detachable inner cylinder 21. A spacer is provided on the upper side of the secondary filter membrane 23. The filter membrane 22 is supported by a connecting seat 12 at the bottom of the support plate 7. A storage tank 8 is connected to the lower side of the connecting seat 12 by a threaded seal. A horizontal fixing plate 16 is fixed to the rear side of the two sets of upright plates 10. An electric push rod 15 is installed on the fixing plate 16. A sliding pressure rod 2 is installed inside the top plate 3. The pressure rod 2 is coaxial with the purification cylinder 5. A pressure plate 4 with the same specifications as the purification cylinder 5 is fixed to the lower end of the pressure rod 2. A pressure sensor 18 is embedded at the bottom of the pressure plate 4. The upper end of the pressure rod 2 is connected to the output end of the electric push rod 15 through a connecting plate 1. This design solves the problem that in the process of phage screening and purification, the phage stock solution is usually poured onto the filter membrane manually and then gravity is used to make the phage stock solution pass through the filter membrane to achieve phage filtration and purification, which is cumbersome and time-consuming.

[0027] In this embodiment, the bottom surface and the ring surface of the pressure plate 4 are covered with a layer of rubber sleeve 17 for sealing. The rubber sleeve 17 has good elasticity and sealing performance, and can fit tightly against the inner surface of the detachable inner cylinder 21 when pressurized, ensuring the sealing shape at the contact surface and effectively preventing gas leakage from affecting the pressurization effect.

[0028] In this embodiment, the support plate 7 is provided with a positioning groove 19 that is compatible with the purification cylinder 5. The connecting pipe 20 is located at the center of the positioning groove 19. The design of the positioning groove 19 provides a precise placement position for the purification cylinder 5, ensuring the stability of the purification cylinder 5 on the support plate 7. It can prevent the purification cylinder 5 from shifting or tilting during the pressurization process, thereby ensuring the smooth progress of the purification process. At the same time, it facilitates the quick connection between the purification cylinder 5 and the connecting pipe 20.

[0029] In this embodiment, the cooling assembly 6 includes an air inlet pipe 61, a quick-connect female connector 62, a connecting hose 63, a sealing and insulating sleeve 64, and a quick-connect male connector 65. The sealing and insulating sleeve 64 has a hollow structure and covers the annular surface of the purification cylinder 5. The connecting hose 63 is connected to the left side of the upper surface of the sealing and insulating sleeve 64, and the quick-connect male connector 65 is installed at the end of the connecting hose 63. The air inlet pipe 61 is installed through the interior of the left vertical plate 10. A quick-connect female connector 62 adapted to the quick-connect male connector 65 is installed on the right end of the air inlet pipe 61, and its left end is connected to an external cooling device. A pressure relief nozzle 66 for pressure relief is installed on the right side of the upper surface of the sealing and insulating sleeve 64. The cooling assembly 6 can introduce cold air to cool the interior of the purification cylinder 5, ensuring that the interior of the purification cylinder 5 is in a low-temperature environment, thereby maintaining the activity of the bacteriophage.

[0030] In this embodiment, the connecting plate 1 is connected to ear plates 13 on both the left and right sides. The top plate 3 is symmetrically fixed with two sets of guide rods 14. The ear plates 13 are sleeved on the guide rods 14 and slidably connected. This design allows the connecting plate 1 to slide stably along the guide rods 14 when it moves up and down, avoiding the shaking or deviation of the connecting plate 1 during the movement, thereby ensuring the stability of the movement of the pressure rod 2 and the pressure plate 4.

[0031] In this embodiment, a layer of anti-slip rubber pad 91 is attached to the bottom of the base plate 9. The anti-slip rubber pad 91 has a good coefficient of friction, which can increase the friction between the base plate 9 and the placement surface, thereby effectively preventing the device from sliding or tilting during use and ensuring the stability of the device.

[0032] In this embodiment, the primary filter membrane 22 is a coarse filter membrane with a pore size of 0.55 μm, which can coarsely filter the phage stock solution and remove larger particulate impurities in the stock solution. The secondary filter membrane 23 is a fine filter membrane with a pore size of 0.22 μm, which performs secondary filtration on the phage stock solution and can remove tiny particulate impurities in the stock solution, ensuring the purification effect of the phage stock solution.

[0033] The working principle of this phage screening and purification device:

[0034] In actual use, first, place the purification cylinder 5 on the support plate 7 and insert its bottom into the positioning groove 19. Insert the connector 24 into the connecting pipe 20. Then, connect the storage pipe to the connector 12 via threads. Next, connect the connecting hose 63 to the quick-connect male connector 65 and quick-connect female connector 62, and connect the air inlet pipe 61 to the external cooling device. Start the external cooling device to introduce cold air into the sealed insulation jacket 64, cooling the purification cylinder 5. Then, pour the phage stock solution to be filtered into the purification cylinder 5. Start the electric push rod 15 via the controller to retract it. The electric push rod 15 moves the pressure rod 2 downwards, which in turn moves the pressure plate 4 downwards and engages it in the purification cylinder 5. The pressure sensor 18 accurately detects pressure changes inside the purification cylinder 5, and the digital display controller 11 displays the pressure inside the purification cylinder 5 in real time. When the pressure inside the purification cylinder 5 reaches an appropriate range, turn off the electric push rod 15. Under pressure, the phage stock solution inside the purification cylinder 5 can quickly pass through the primary filter membrane 22 and the secondary filter membrane 23, and then flow into the storage tank 8 through the connecting pipe 20, thereby achieving rapid filtration and purification of the phage stock solution.

[0035] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. For those skilled in the art, other variations or modifications can be made based on the above description. It is impossible to exhaustively list all the implementation methods here. Any obvious variations or modifications derived from the technical solutions of this utility model are still within the protection scope of this utility model.

Claims

1. A phage selection purification device, characterized by: Including the bottom plate (9) and the stand (10), both sides edges of the upper end surface of the bottom plate (9) are fixed with the stand (10), the right side stand (10) is installed with digital display controller (11), two groups of the stand (10) are fixed with support plate (7) between, two groups of the stand (10) top are fixed with top plate (3), the inside vertical through fixed with the communicating pipe (20) of support plate (7), the support plate (7) is placed with the purification cylinder (5) on the communicating pipe (20), the outside of the purification cylinder (5) is provided with cooling assembly (6), the bottom of the purification cylinder (5) is funnel structure, and the bottom is fixed with the plug-in pipe (24) matched with the communicating pipe (20), the plug-in pipe (24) is detachably plugged with the communicating pipe (20), the inside of the purification cylinder (5) is provided with detachable inner cylinder (21) on the upside, the detachable inner cylinder (21) bottom is provided with two-stage filter membrane (23), the two-stage filter membrane (23) upside is provided with one-stage filter membrane (22) with interval, the bottom of the support plate (7) is fixed with connecting seat (12), the lower side of the connecting seat (12) is sealed with the liquid storage tank (8) through thread, the rear side of two groups of the stand (10) is fixed with horizontal fixed plate (16), the fixed plate (16) is installed with electric push rod (15), the inside of the top plate (3) is provided with the pressure rod (2) that can slide up and down, the pressure rod (2) is coaxial with the purification cylinder (5), the lower end of the pressure rod (2) is fixed with the pressure plate (4) matched with the specification of the purification cylinder (5), the bottom of the pressure plate (4) is embedded with pressure sensor (18), the upper end of the pressure rod (2) is connected with the output end of electric push rod (15) through the connecting handle (1).

2. The phage display biopanning device of claim 1, wherein: The bottom surface and the annular surface of the pressure plate (4) are covered with a layer of sealing rubber sleeve (17).

3. The phage display biopanning device of claim 1, wherein: A positioning groove (19) matched with the purification cylinder (5) is formed in the support plate (7), and the communicating pipe (20) is located at the center of the positioning groove (19).

4. The phage display biopanning device of claim 1, wherein: The cooling assembly (6) comprises an air inlet pipe (61), a quick female connector (62), a connecting hose (63), a sealing heat preservation sleeve (64), and a quick male connector (65). The sealing heat preservation sleeve (64) is a hollow structure that covers the annular surface of the purification cylinder (5). The left side of the upper surface of the sealing heat preservation sleeve (64) is connected with the connecting hose (63). The end of the connecting hose (63) is installed with the quick male connector (65). The inside of the left side of the stand (10) is provided with the air inlet pipe (61). The right end of the air inlet pipe (61) is installed with the quick female connector (62) matched with the quick male connector (65). The left end is connected to an external refrigeration device. The right side of the upper surface of the sealing heat preservation sleeve (64) is installed with a pressure relief nozzle (66) for pressure relief.

5. The phage display biopanning device of claim 1, wherein: The connecting handle (1) is connected with an ear plate (13) on both sides. The top plate (3) is fixed with two groups of guide struts (14) on the upper surface. The ear plate (13) is sleeved on the guide struts (14) and is in sliding connection with them.

6. The phage display biopanning device of claim 1, wherein: A layer of anti-skid rubber pad (91) is attached to the bottom of the bottom plate (9).

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