Dunaliella salina cell bioreactor

Abstract

A dunaliella salina cell bioreactor comprises a glass reactor and is characterized in that a top cover is mounted at the top end of the glass reactor, a motor is arranged at the edge of the top end of the top cover, a rotating shaft is connected to the bottom end of the motor, an outer gear is connected to the bottom end of the rotating shaft, and an inner gear is connected to the outer side of the outer gear; a protruding block is arranged on the outer side of the inner gear, a fixing ring is connected to the outer side of the protruding block, a groove is formed in the inner side of the fixing ring, a plurality of steel balls are arranged at the bottom end of the groove, a connecting block is connected to the bottom end of the inner gear, a connecting block is installed at the bottom end of the connecting block, and stirring strips are installed at the edge of the bottom end of the mounting ring. Water flow can slowly fluctuate to strengthen mixing, dunaliella salina cells are not easy to aggregate and precipitate under the condition that shearing force is reduced as far as possible, and the phenomenon of cell attachment can be solved by rotating the stirring strips along the inner wall of the glass reactor.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, specifically relating to a Dunaliella salina cell bioreactor. Background Technology

[0002] Dunaliella salina is a biflagellate, single-celled green algae lacking a complete cell wall structure, distributed in oceans, salt lakes, and salt pans. Discovered in the Dead Sea by Israeli biologist Professor Amoz, research revealed that this algae, which originated 3.8 billion years ago, is one of the earliest forms of life, capable of surviving temperatures as high as 53°C and reproducing in temperatures as low as -27°C. Known for its tolerance to high salinity, Dunaliella salina thrives in the Dead Sea, the third saltiest lake in the world, where even bacteria cannot survive, having survived five mass extinctions. Experiments have shown that it can tolerate salinity ranging from 3% to 30%. It is rich in over 70 essential nutrients, minerals, and trace elements, including various natural carotenoids, essential amino acids, folic acid, vitamin A, vitamin E, linolenic acid, linoleic acid, lecithin, fucoidan, dietary fiber, calcium, iron, zinc, and selenium.

[0003] Cells tend to aggregate and precipitate in high-salt environments, requiring enhanced mixing. However, ordinary spiral stirring or airlift mixing may cause adhesion to the cell walls. To address this, we propose a Dunaliella salina cell bioreactor. Utility Model Content

[0004] The purpose of this invention is to provide a Dunaliella salina cell bioreactor to solve the problem mentioned in the background art that cells tend to aggregate and precipitate under high-salt conditions, requiring enhanced mixing, but ordinary spiral stirring or airlift mixing may cause adhesion to the walls.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a Dunaliella salina cell bioreactor, comprising a glass reactor, characterized in that: a top cover is installed at the top of the glass reactor, a motor is provided at the top edge of the top cover, a rotating shaft is connected to the output end of the motor, an external gear is connected to the bottom end of the rotating shaft, an internal gear is connected to the outer side of the external gear, a protrusion is provided on the outer side of the internal gear, a fixing ring is connected to the outer side of the protrusion, a groove is provided on the inner side of the fixing ring, a plurality of steel balls are provided at the bottom end of the groove, a connecting block is connected to the bottom end of the internal gear, an installation ring is installed at the bottom end of the connecting block, and a stirring strip is installed at the bottom edge of the installation ring.

[0006] Preferably, the glass reactor has an outlet at its lower edge, a support frame at its bottom, an inlet on one side of the top of the top cover, and a control unit connector at the center of the top of the top cover.

[0007] Preferably, the rotating shaft is fixedly connected to the external gear, and the external gear meshes with the internal gear, forming a linkage structure between the external gear and the internal gear.

[0008] Preferably, the outer diameter of the protrusion matches the inner diameter of the groove, and the protrusion and the fixing ring are slidably connected through the groove, with the steel balls evenly distributed at the bottom of the groove.

[0009] Preferably, the mounting ring is fixedly connected to the internal gear via a connecting block, and the mounting ring and the internal gear form a linkage structure, and the stirring bar is fixedly connected to the mounting ring.

[0010] Preferably, the outer diameter of the mounting ring is the same as the inner diameter of the glass reactor, and the stirring bar can rotate along the inner wall of the glass reactor.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] This Dunaliella salina cell bioreactor has a rotating device inside the top cover. Driven by a motor, the stirring bar rotates slowly along the inner wall of the glass reactor, which can make the water flow fluctuate slowly to enhance mixing. This reduces the shear force as much as possible, making it less likely for Dunaliella salina cells to aggregate and precipitate. The rotation of the stirring bar along the inner wall of the glass reactor can solve the problem of cell adhesion, reducing the workload of subsequent cleaning. Attached Figure Description

[0013] Figure 1 This is a three-dimensional schematic diagram of the present invention;

[0014] Figure 2 This is a front sectional view of the top cover of this utility model;

[0015] Figure 3 This utility model Figure 1 Enlarged view of point A in the middle;

[0016] Figure 4 This is a top sectional view of the internal gear of this utility model.

[0017] In the diagram: 1. Glass reactor; 12. Outlet; 2. Top cover; 21. Inlet; 22. Controller connector; 3. Motor; 31. Rotating shaft; 32. External gear; 33. Fixing ring; 331. Groove; 332. Steel ball; 34. Internal gear; 341. Protrusion; 342. Connecting block; 343. Mounting ring; 344. Stirring bar; 4. Support frame. Detailed Implementation

[0018] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0019] Please see Figure 1-4 This utility model provides a technical solution: a Dunaliella salina cell bioreactor, comprising a glass reactor 1, characterized in that: an outlet 12 is provided at the lower edge of the glass reactor 1 for discharging water and collecting Dunaliella salina cells; a support frame 4 is provided at the bottom of the glass reactor 1 for support; a top cover 2 is installed at the top of the glass reactor 1 to seal the space and prevent contamination; an inlet 21 is provided on one side of the top of the top cover 2 for water intake; and a control connector 22 is provided at the center of the top of the top cover 2 for various adjustment functions. The internal data of reactor 1 is used to control the rotation of the internal gear. A motor 3 is installed at the top edge of the top cover 2. The output end of the motor 3 is connected to a rotating shaft 31, which serves as a connection. An external gear 32 is connected to the bottom end of the rotating shaft 31, and an internal gear 34 is connected to the outside of the external gear 32. The rotating shaft 31 and the external gear 32 are fixedly connected, and the external gear 32 and the internal gear 34 mesh, forming a linkage structure. The motor 3 drives the internal gear 34 to rotate through the external gear 32. A protrusion 341 is provided on the outside of the internal gear 34, and a retaining ring is connected to the outside of the protrusion 341. 33. A retaining ring 33 is fixedly installed on the inner side of the top cover 2. A groove 331 is provided on the inner side of the retaining ring 33. Several steel balls 332 are provided at the bottom end of the groove 331. The outer diameter of the protrusion 341 matches the inner diameter of the groove 331, and the protrusion 341 and the retaining ring 33 are slidably connected through the groove 331. The steel balls 332 are evenly distributed at the bottom end of the groove 331. With the assistance of the steel balls 332, the internal gear 34 rotates and slides inside the groove 331. A connecting block 342 is connected to the bottom end of the internal gear 34, serving a connecting function. The connecting block 342... An installation ring 343 is installed at the bottom. The installation ring 343 is fixedly connected to the internal gear 34 via a connecting block 342, and the installation ring 343 and the internal gear 34 form a linkage structure. The installation ring 343 rotates synchronously with the internal gear 34. A stirring bar 344 is installed at the bottom edge of the installation ring 343. The stirring bar 344 is fixedly connected to the installation ring 343 and plays a stirring role. The outer diameter of the installation ring 343 is the same as the inner diameter of the glass reactor 1, and the stirring bar 344 can rotate and adhere to the inner wall of the glass reactor 1, which can solve the problem of cell adhesion.

[0020] Working Principle: For this type of Dunaliella salina cell bioreactor, the operator first transports the culture medium and Dunaliella salina cells from the inlet 21 into the glass reactor 1, then connects the controller pipe 22. After adjusting various data via the control host, the PLC controller starts the motor 3, which slowly rotates. The motor 3 drives the external gear 32 to rotate, which in turn drives the internal gear 34. The stirring bar 344 then rotates along the wall, causing the water flow to ripple slowly, thus mixing the Dunaliella salina cells. This minimizes shear force and prevents the cells from agglomerating and settling. The stirring bar rotates along the inner wall of the glass reactor, increasing the flow of the culture medium on the outer side and preventing cell adhesion. After cell culture is complete, the Dunaliella salina can be collected from the outlet 12. The motor model mentioned above is YS-7116; this model is for reference only and should meet the specific operational requirements.

[0021] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A Dunaliella cell bioreactor comprising a glass reactor (1), characterized in that: The top end of the glass reactor (1) is provided with a top cover (2), the top end edge of the top cover (2) is provided with a motor (3), the output end of the motor (3) is connected with a rotating shaft (31), the bottom end of the rotating shaft (31) is connected with an external gear (32), the outer side of the external gear (32) is connected with an internal gear (34), the outer side of the internal gear (34) is provided with a protruding block (341), the outer side of the protruding block (341) is connected with a fixed ring (33), the inner side of the fixed ring (33) is provided with a groove (331), the bottom end of the groove (331) is provided with a plurality of steel balls (332), the bottom end of the internal gear (34) is connected with a connecting block (342), the bottom end of the connecting block (342) is provided with a mounting ring (343), the bottom end edge of the mounting ring (343) is provided with a stirring bar (344).

2. The Dunaliella cell bioreactor according to claim 1, wherein: The bottom end edge of the glass reactor (1) is provided with a water outlet (12), the bottom end of the glass reactor (1) is provided with a support frame (4), the top end side of the top cover (2) is provided with a water inlet (21), the top center of the top cover (2) is provided with a control instrument connector (22).

3. The Dunaliella cell bioreactor according to claim 1, wherein: The rotating shaft (31) and the external gear (32) are fixedly connected, and the external gear (32) and the internal gear (34) are engaged, and the external gear (32) and the internal gear (34) constitute a linkage structure.

4. The Dunaliella cell bioreactor according to claim 1, wherein: The outer diameter size of the protruding block (341) is matched with the inner diameter size of the groove (331), and the protruding block (341) and the fixed ring (33) are slidably connected through the groove (331), and the steel balls (332) are uniformly distributed at the bottom end of the groove (331).

5. The Dunaliella cell bioreactor according to claim 1, wherein: The mounting ring (343) is fixedly connected with the internal gear (34) through the connecting block (342), and the mounting ring (343) and the internal gear (34) constitute a linkage structure, and the stirring bar (344) is fixedly connected with the mounting ring (343).

6. The Dunaliella cell bioreactor according to claim 1, wherein: The outer diameter size of the mounting ring (343) is the same as the inner diameter size of the glass reactor (1), and the stirring bar (344) can rotate on the inner side of the glass reactor (1).

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