Membrane preparation device for peptide fragment solution purification and experimental assembly

Abstract

The utility model discloses a diaphragm preparation device for peptide fragment solution purification, which comprises a cutting tube and a pressing needle, and the lower end of the cutting tube is a cutting end; the pressing needle comprises a needle body and a handheld part, and the handheld part is located outside the cutting pipe. The needle body is connected with the cutting tube through the bouncy ball, the bouncy ball is fixedly arranged on the needle body in a penetrating manner, the bouncy ball is positioned in the cutting tube, and the needle body is matched with the inner wall of the cutting tube to clamp the bouncy ball, so that the bouncy ball generates elastic deformation; the lower end of the needle body is provided with a flattening structure capable of entering and exiting the cutting pipe, and the lower end face of the flattening structure is a plane inclined relative to the horizontal plane. According to the membrane preparation device for peptide fragment solution purification, the elastic ball enables the cutting tube and the pressing needle to be connected together, and the solid-phase extraction membrane does not need to be separated before, during and after being cut, so that pollution to the cutting tube, the pressing needle and the solid-phase extraction membrane caused by repeated separation of the cutting tube and the pressing needle is avoided; and a clean solid-phase extraction membrane can be obtained.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biological laboratory consumables, more particularly, it relates to a membrane preparation device for peptide segment solution purification and experimental assembly. BACKGROUND

[0002] The solid-phase extraction membrane is composed of functional particles fixed by high molecular fiber mesh, and has excellent physical properties (such as softness and compactness) and chemical properties (such as high retention factor), so that Stage Tips are particularly suitable for processing peptide solution samples before LC / MS analysis.

[0003] The membrane prepared by the membrane preparation device can intercept peptide segments generated by protein decomposition in a micropipette, and is commonly used in proteomics experiments that highly depend on excellent sample pretreatment stages. However, the conventional membrane preparation device uses a cutting tube and a pressing needle to cut and obtain solid-phase extraction membranes of the required diameter. The cutting tube and the pressing needle are initially separated, and after the cutting tube cuts the solid-phase extraction membrane of the required diameter, the pressing needle is inserted into the cutting tube to push the cut membrane out of the cutting tube, so that the membrane falls into the pipette. Then the pressing needle is used to flatten the membrane in the pipette. After that, the pressing needle is taken out of the cutting tube, and the two are stored separately. The separated cutting tube and pressing needle, especially the end of the pressing needle used to press the membrane, are easy to be contaminated by environmental stains. The contaminated stains may also adhere to the cut solid-phase extraction membrane, causing the void of the solid-phase extraction membrane to be blocked, which makes the solid-phase extraction membrane unusable.

[0004] In addition, the cutting tube needs to be pressed and twisted to cut the solid-phase extraction membrane. If the pressing force or the twisting amplitude is insufficient, the cut solid-phase extraction membrane may not be lifted. At the same time, the adsorption force of the cutting tube is insufficient, which causes the solid-phase extraction membrane to fall off from the cutting tube before entering the pipette. The fallen solid-phase extraction membrane is contaminated and cannot be recycled.

[0005] In addition, the pressing needle of the conventional membrane preparation device is too long, and under excessive force, the long pressing needle often causes the solid-phase extraction membrane to be placed too low in the pipette. The pipette with the solid-phase extraction membrane needs to be loaded for centrifugation during use. The low solid-phase extraction membrane may be thrown out of the lower end of the pipette during centrifugation, causing loss of peptide segments.

[0006] Finally, the tip of the conventional pressing needle has a certain adhesion, and the solid-phase extraction membrane in contact with the tip may adhere to the tip, making it difficult to put the solid-phase extraction membrane adhered to the tip back into the pipette. SUMMARY

[0007] In view of the above defects or improvement needs of the prior art, the utility model provides a membrane piece preparation device and experimental assembly for peptide segment solution purification, when the cutting pipe cuts the solid phase extraction membrane piece, the elastic ball provides a suction force, the solid phase extraction membrane piece is cut better, and meanwhile, the cut solid phase extraction membrane piece is not easy to fall off.

[0008] In order to achieve the above object, according to the utility model, a membrane piece preparation device for peptide segment solution purification is provided, comprising a cutting pipe and a pressing needle, characterized in that:

[0009] The cutting pipe is vertically arranged, and the lower end of the cutting pipe is a cutting end to obtain the solid phase extraction membrane piece of the required diameter by cutting;

[0010] The pressing needle comprises a cylindrical needle body and a hand holding part arranged at the upper end of the needle body, the needle body is vertically arranged, and the hand holding part is located outside the cutting pipe;

[0011] The needle body is connected with the cutting pipe through an elastic ball, the elastic ball is fixed on the needle body, the elastic ball is located in the cutting pipe, and the needle body and the inner wall of the cutting pipe clamp the elastic ball to make the elastic ball elastically deform;

[0012] The lower end of the needle body is provided with a flattening structure that can enter and exit the cutting pipe, the lower end surface of the flattening structure is a plane, and the included angle between the lower end surface of the flattening structure and the axis of the needle body is greater than 0° and less than 90°.

[0013] Preferably, the flattening structure is a structure obtained by a plane truncated cone, the included angle between the plane of the truncated cone and the axis of the circular cone is greater than 0° and less than 90°, so that the lower end surface of the flattening structure is an ellipse as a whole, and the circular cone is coaxially arranged with the needle body.

[0014] Preferably, the elastic ball is integrally formed with the needle body.

[0015] Preferably, the axial length of the pressing needle is 6.8cm-7.2cm.

[0016] Preferably, the flattening structure is integrally formed with the needle body.

[0017] Preferably, the flattening structure is a solid structure.

[0018] Preferably, the elastic ball is made of rubber or silicone.

[0019] Preferably, the flattening structure is made of plastic or metal that will not stick to the solid phase extraction membrane piece.

[0020] According to another aspect of the utility model, still provide the experimental assembly for peptide segment solution purification, including the membrane preparation device, its characterized in be further including pipette, the pipette content has the solid phase extraction membrane piece that exits from cutting pipe.

[0021] Preferably, the solid phase extraction membrane is an Empore TM solid phase extraction membrane.

[0022] Overall, the above technical scheme conceived by the utility model compared with the prior art can achieve the following beneficial effects:

[0023] 1) The membrane preparation device for peptide segment solution purification of the utility model, the elastic ball can connect the cutting tube and the pressing needle together, and the cutting tube and the pressing needle do not need to be separated before, during and after cutting the solid phase extraction membrane, avoiding the pollution of the cutting tube, the pressing needle and the solid phase extraction membrane caused by repeated separation of the cutting tube and the pressing needle.

[0024] 2) The membrane preparation device for peptide segment solution purification of the utility model, the elastic ball generates elastic deformation to seal the cutting tube, the cutting tube, the elastic ball and the pressing needle are combined into a structure similar to a syringe, and after the solid phase extraction membrane enters the cutting tube, the pressing needle can be slightly pulled to provide a certain suction force, so that the solid phase extraction membrane is not easy to fall out of the cutting tube, and the success rate of obtaining the solid phase extraction membrane is greatly improved.

[0025] 3) The membrane preparation device for peptide segment solution purification of the utility model, the flattening structure adopts plastic or metal that will not stick to the solid phase extraction membrane, and does not adopt the rubber structure at the bottom of the conventional pressing needle, directly changes the rubber structure at the bottom of the conventional pressing needle into plastic or metal material that will not stick to the solid phase extraction membrane, avoids the waste of the solid phase extraction membrane under the premise of retaining the effect of flattening the solid phase extraction membrane.

[0026] 4) The membrane preparation device for peptide segment solution purification of the utility model, by adjusting the length of the pressing needle, ensures that the solid phase extraction membrane is fixed at the best position of the pipette, and avoids the risk of sample loss caused by the solid phase extraction membrane being thrown out during the use of the Tip column.

[0027] 5) The experimental assembly for peptide segment solution purification of the utility model, after the membrane preparation device cuts the solid phase extraction membrane, the cut solid phase extraction membrane can be transferred into the pipette, the operation is simple and reliable, and the obtained solid phase extraction membrane will not be polluted and wasted. BRIEF DESCRIPTION OF DRAWINGS

[0028] Fig. 1 It is a structural schematic view of the utility model;

[0029] Fig. 2 It is a schematic view of the cutting tube in the utility model;

[0030] Fig. 3 It is the schematic view of connecting elastic ball on pressing needle in the utility model;

[0031] Fig. 4 It is the schematic view of containing solid phase extraction membrane in pipette in the utility model. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantage of the utility model more clearly, the following is in combination with the drawings and embodiment, and the utility model is further detailed.The specific embodiments described here are only used to explain the utility model, and are not used to limit the utility model.In addition, the technical features involved in each embodiment of the utility model described below can be combined with each other as long as they do not conflict with each other.

[0033] Referring to Figs. 1-4 The membrane preparation device for peptide segment solution purification includes cutting tube 1 and pressing needle 2, the cutting tube 1 of the utility model is the cutting tube 1 used for preparing Stage Tips (Stop and go extraction tips) normally, the structure is not changed, Stage Tips is micro pipette 4 filled with solid phase extraction membrane 5, also called solid phase extraction suction head or gun head membrane filter column.

[0034] The cutting tube 1 is vertically arranged, and the lower end of the cutting tube 1 is a cutting end 11, so that the required diameter solid phase extraction membrane 5 is obtained by cutting.The cutting end 11 of the cutting tube 1 cuts small solid phase extraction membrane 5 into the cutting tube 1 by pressing on a large solid phase extraction membrane 5.

[0035] The pressing needle 2 includes cylindrical needle body 21 and hand holding part 22 arranged on the upper end of the needle body 21, the hand holding part 22 is provided with anti-skid lines, facilitating holding;The needle body 21 is vertically arranged, and the hand holding part 22 is located outside the cutting tube 1;The total length of the pressing needle 2 is greater than the total length of the cutting tube 1, and the lower end of the pressing needle 2 can enter and exit the cutting tube 1.

[0036] The needle body 21 is connected with the cutting tube 1 through the elastic ball 3, and the elastic ball 3 is fixed on the needle body 21, and the elastic ball 3 is integrally formed with the pressing needle 2, and can be integrally formed by injection molding. The elastic ball 3 is made of rubber or silicone, and other elastic polymer materials can also be used to prepare the elastic ball 3. The elastic ball 3 is located in the cutting tube 1, and the needle body 21 and the inner wall of the cutting tube 1 clamp the elastic ball 3 to make the elastic ball 3 elastically deform. The elastic ball 3 is elastic and is an elastic rubber ball. The elastic ball 3 can be associated with the pressing needle 2 and inserted into the cutting tube 1 to complete sealing, forming a structure similar to a syringe. The elastic ball 3 corresponds to the piston of the syringe. When the cutting tube 1 cuts the membrane, the elastic ball 3 can be lifted to provide a suction force, which can better cut the large solid phase extraction membrane 5. At the same time, the small solid phase extraction membrane 5 cut in the cutting tube 1 is not easy to fall off in the cutting tube 1.

[0037] The lower end of the needle body 21 is provided with a flattening structure 23 which can enter and exit the cutting tube 1. The flattening structure 23 is integrally formed with the pressing needle 2, which is convenient for molding. The flattening structure 23 is preferably a solid structure, which is convenient for pressing. The lower end surface of the flattening structure 23 is a plane, and the angle between the lower end surface of the flattening structure 23 and the axis of the needle body 21 is greater than 0° and less than 90°, and the two are neither parallel nor perpendicular. The flattening structure 23 can push the solid phase extraction membrane 5 in the cutting tube 1 out of the cutting tube 1, so that the solid phase extraction membrane 5 enters the pipette 4 from the cutting tube 1, and then the plane of the lower end surface of the flattening structure 23 can adjust the solid phase extraction membrane 5 in the pipette 4 to a horizontal state. Since there is an elastic ball 3 between the pressing needle 2 and the cutting tube 1, the pressing needle 2 can swing and shake during use to adjust the lower end surface of the flattening structure 23 to a horizontal state, so that the lower end surface of the flattening structure 23 can adjust the solid phase extraction membrane 5 in the pipette 4 to a horizontal state.

[0038] Further, the flattening structure 23 is a structure obtained by cutting a circular truncated cone, and the angle between the plane of the circular truncated cone and the axis of the circular truncated cone is greater than 0° and less than 90°, so that the lower end surface of the flattening structure 23 is elliptical as a whole, and the circular truncated cone is coaxially arranged with the needle body 21. The upper end surface of the flattening structure 23 is consistent with the diameter of the needle body 21.

[0039] Further, the axial length of the pressing needle 2 is 6.8cm-7.2cm, preferably 7cm. This length makes the solid phase extraction membrane 5 reach a suitable position after the pressing needle 2 is completely pressed down.

[0040] According to another aspect of the present application, there is also provided an experimental assembly for purification of a peptide segment solution, comprising a membrane preparation device and a pipette 4, the pipette 4 containing a solid phase extraction membrane 5 which is withdrawn from a cutting tube 1. The solid phase extraction membrane 5 is preferably an Empore TM solid phase extraction membrane.

[0041] Compared with the conventional pressing needle 2, the bottom of the pressing needle 2 of the present application removes the conventional colloid structure and is replaced by an irregular circular truncated cone flat structure 23, and the needle body 21 also has a resilient ball 3. Since the resilient ball 3 is spherical, the pressing needle 2 has a certain moving and swinging amplitude, the slightly moving of the flat structure 23 at the bottom of the pressing needle 2 can flatten the solid phase extraction membrane 5 in the pipette 4.

[0042] Those skilled in the art will readily understand that the above description is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A membrane preparation apparatus for purifying peptide solutions, comprising a cutting tube and a pressing needle, characterized in that: The cutting tube is set vertically, and the lower end of the cutting tube is the cutting end, so as to obtain a solid phase extraction membrane of the required diameter by cutting. The pressing needle includes a cylindrical needle body and a hand grip portion disposed at the upper end of the needle body. The needle body is vertically arranged, and the hand grip portion is located outside the cutting tube. The needle body is connected to the cutting tube via an elastic ball, and the elastic ball is fixedly mounted on the needle body. The elastic ball is located inside the cutting tube, and the needle body and the inner wall of the cutting tube cooperate to clamp the elastic ball so that the elastic ball undergoes elastic deformation. The lower end of the needle body is provided with a flattening structure that allows the cutting tube to enter and exit. The lower end surface of the flattening structure is a plane, and the angle between the lower end surface of the flattening structure and the axis of the needle body is greater than 0° and less than 90°.

2. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The flattening structure is a structure obtained by cutting a frustum of a circle with a plane. The angle between the plane of the frustum and the axis of the frustum is greater than 0° and less than 90°, so that the lower end face of the flattening structure is elliptical as a whole. The frustum and the needle body are coaxially arranged.

3. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The elastic ball is integrally formed with the needle body.

4. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The axial length of the pressing needle is 6.8cm to 7.2cm.

5. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The flattening structure is integrally formed with the needle body.

6. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The flattening structure is a solid structure.

7. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The bouncy ball is made of rubber or silicone.

8. The membrane preparation apparatus for peptide solution purification according to claim 1, characterized in that, The flattening structure is made of plastic or metal that will not stick to the solid phase extraction membrane.

9. An experimental assembly for purifying peptide solutions, comprising the membrane preparation apparatus according to any one of claims 1 to 8, characterized in that, It also includes a pipette containing a solid-phase extraction membrane that has exited from the cutting tube.

10. The experimental setup for purifying peptide solutions according to claim 9, characterized in that, The solid phase extraction membrane is an Empore™ solid phase extraction membrane.

Images