Rapid detection device for broad-affinity pollen activity of rice

Abstract

The utility model relates to the technical field of rice pollen viability detection, and particularly discloses a rice broad-affinity pollen viability rapid detection device, after pollen falls into an adsorption plate through a feed hopper, an adsorption mechanism fixes the pollen in adsorption holes through negative pressure, and a vibration mechanism drives the adsorption plate to vibrate, so that the pollen is uniformly distributed and embedded into the adsorption holes; accumulation is avoided; unfixed redundant pollen is blown away from the adsorption plate by the air blowing mechanism, so that background interference is eliminated; the detection mechanism is used for quickly scanning and analyzing the fixed pollen. Through cooperative control of adsorption, vibration, purging and detection, automatic positioning, impurity removal and high-throughput detection of pollen are realized, the problems of low manual sample spreading efficiency, large residue interference and scattered detection process in a traditional method are solved, and the detection speed and accuracy are remarkably improved.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of rice pollen viability detection, in particular to a rice general compatibility pollen viability rapid detection device. BACKGROUND

[0002] Rice general compatibility pollen is a key element for improving parent compatibility and hybridization efficiency in hybrid rice breeding because it can break the reproductive isolation between indica and japonica subspecies. Pollen viability directly affects the pollination success rate and the seed setting rate of hybrid offspring. In particular, during the screening process of general compatibility varieties, rapid and accurate detection of pollen viability is a core requirement for optimizing parent combinations and improving seed production efficiency. However, traditional methods are difficult to meet the requirements of high-throughput and high-precision detection in modern breeding due to long detection period, complex operation and large environmental interference, and more efficient detection techniques are urgently needed.

[0003] Current mainstream detection techniques include in vitro culture method and staining microscopy. The in vitro culture method requires placing pollen in culture medium to observe germination, which takes 24-48 hours, and is complicated to operate and easily affected by temperature and humidity. The staining microscopy relies on TTC or fluorescent staining agent combined with manual microscope observation, which shortens the time, but requires manual sorting, staining and sample-by-sample interpretation, which has large subjective error and low efficiency. Although some automated equipment attempts to replace manual analysis with microscopic imaging, it still requires manual sample preparation, and uneven pollen distribution, impurities and other problems lead to insufficient reliability of detection results.

[0004] The existing technology has the following bottlenecks: first, sample processing relies on manual sample preparation, pollen is prone to accumulation or uneven distribution, leading to insufficient detection representation; second, unfixed or residual pollen is difficult to remove, causing background interference; third, the staining process relies on manual operation, and the detection conditions are difficult to standardize, making it impossible to achieve batch continuous detection; fourth, existing equipment lacks integrated integration, resulting in long detection period and high cost. These problems seriously restrict the efficiency and accuracy of general compatibility pollen viability detection, and urgent technical innovation is needed to solve them. CONTENT OF THE INVENTION

[0005] In order to solve the defects in the above detection, the application provides a rice general compatibility pollen viability rapid detection device.

[0006] The rice general compatibility pollen viability rapid detection device provided by the application adopts the following technical solutions:

[0007] The utility model provides a kind of rice broad affinity pollen activity rapid detection device, including detection table, the detection table is provided with adsorption plate, a plurality of adsorption holes are opened in the adsorption plate, adsorption mechanism is provided in the detection table below the adsorption plate, top plate is provided above the adsorption plate, top plate is provided with feed hopper, the detection table is provided with the vibration mechanism for making the adsorption plate vibrate, the adsorption plate side is also provided with the air blowing mechanism for blowing the excess pollen that the adsorption plate is not fallen into the adsorption hole to the adsorption plate outside, the top plate side close to the adsorption plate is provided with the detection mechanism for detecting pollen activity.

[0008] By adopting the above technical scheme, pollen falls into the adsorption plate through the feed hopper, the adsorption mechanism fixes the pollen in the adsorption hole through negative pressure, the vibration mechanism drives the adsorption plate to vibrate, to make the pollen evenly distributed and embedded in the adsorption hole, avoiding accumulation; The air blowing mechanism blows the unfixed excess pollen away from the adsorption plate, eliminating background interference; The detection mechanism rapidly scans and analyzes the fixed pollen. Through the coordinated control of adsorption, vibration, purging and detection, automatic positioning, impurity removal and high-throughput detection of pollen are realized, solving the problems of low efficiency, large residual interference and dispersed detection process in traditional methods, and significantly improving the detection speed and accuracy.

[0009] Optionally, the adsorption mechanism includes a plurality of air extractors arranged in the detection table, a cavity is formed in the detection table above the adsorption plate, the cavity is divided into a plurality of suction chambers of uniform size, and each air extractor is arranged in a corresponding suction chamber.

[0010] By adopting the above technical scheme, the cavity is divided into a plurality of uniform suction chambers, and each chamber is configured with an independent air extractor, so that the negative pressure distribution of different regions of the adsorption plate is uniform and controllable. Through zoned independent suction, it is ensured that the adsorption force of the adsorption hole on the pollen is consistent, avoiding uneven distribution or falling of the pollen caused by local overstrong or weak adsorption, thereby ensuring the representativeness of the sample and the reliability of the data in subsequent detection, and overcoming the problem of uneven sample coverage caused by traditional single-point adsorption.

[0011] Optionally, the vibration mechanism includes a vibration motor arranged on the detection table, and the vibration motor can be used to generate vibration of the adsorption plate.

[0012] By adopting the above technical scheme, the vibration motor generates high-frequency micro-amplitude vibration, which is transmitted to the adsorption plate to force the pollen to move into the adsorption hole under the action of inertia and tightly fit. Through mechanical vibration, the pollen is quickly and evenly embedded in the adsorption hole, reducing manual intervention, while avoiding detection errors caused by pollen accumulation or loose distribution, significantly improving the sample fixation efficiency and the standardization degree of the detection area.

[0013] Optionally, the air blower mechanism comprises an air blower, an air expansion cavity and air blow nozzles, the air blower is installed on the detection table, the air expansion cavity is arranged on one side of the adsorption plate and communicates with the air blower, the air blow nozzles are arranged in multiple, each air blow nozzle is uniformly designed on the outer wall of the air expansion cavity and communicates with the inner cavity of the air expansion cavity, and the other side of the adsorption plate where the air blow nozzles are arranged is provided with a collection mechanism for collecting the pollen blown off the adsorption plate.

[0014] By adopting the above technical scheme, the air blower inputs airflow into the air expansion cavity, forms directional and continuous airflow layer through the uniformly distributed air blow nozzles, covers the surface of the adsorption plate and sweeps the unfixed pollen to the collection mechanism. The residual pollen is completely removed by the uniform airflow, preventing it from interfering with the detection results, and the collection mechanism recycles the swept material to avoid pollution, solving the problems of low manual cleaning efficiency and easy omission in the traditional method, and ensuring the cleanliness and repeatability of the detection environment.

[0015] Optionally, the collection mechanism comprises a collection bucket and a collection bag, the collection bucket is arranged on one side of the adsorption plate in an inclined manner and faces each air blow nozzle, and the collection bag is detachably arranged at the lower end of the collection bucket and communicates with the collection bucket.

[0016] By adopting the above technical scheme, the pollen blown out by the air blow nozzle slides along the inclined surface of the collection bucket to the collection bag under the action of airflow, and the collection bag can be quickly detached and replaced. The impurities are efficiently recycled and centrally processed, avoiding the pollution of pollen scattering, and the detachable design facilitates cleaning and maintenance, reduces operation cost, and improves the stability of continuous operation of the device.

[0017] Optionally, the detection mechanism comprises ultraviolet lamps and industrial cameras, the ultraviolet lamps are arranged in multiple, each ultraviolet lamp is uniformly arranged on one side of the top plate close to the adsorption plate, the industrial cameras are arranged in multiple, each industrial camera is arranged on one side of the top plate close to the adsorption plate and is interspersed between the ultraviolet lamps.

[0018] By adopting the above technical scheme, the ultraviolet lamps excite the endogenous fluorescent substances in the pollen, the industrial cameras synchronously shoot the fluorescent signals and quantify the vitality level through image analysis algorithm. The non-destructive fluorescent detection technology is used to avoid the influence of the dyeing step on the activity of the pollen, and the multiple cameras are interspersed to realize rapid scanning of the whole area, combined with automatic image processing, which significantly improves the detection speed and objectivity and overcomes the defects of time-consuming, strong subjectivity and sample destruction of the traditional dyeing method.

[0019] In summary, the present application has at least one of the following beneficial technical effects:

[0020] The pollen falls into the adsorption plate through the feeding hopper, the adsorption mechanism fixes the pollen in the adsorption hole through negative pressure, the vibration mechanism drives the adsorption plate to vibrate, and the pollen is uniformly distributed and embedded in the adsorption hole to avoid accumulation; the air blowing mechanism blows away the unfixed excess pollen from the adsorption plate to eliminate background interference; the detection mechanism quickly scans and analyzes the fixed pollen, and through the cooperative control of adsorption, vibration, blowing and detection, the automatic positioning, impurity removal and high-throughput detection of the pollen are realized, the problems of low efficiency, large residual interference and dispersed detection process in the traditional method are solved, and the detection speed and accuracy are significantly improved;

[0021] The cavity is divided into multiple uniform suction chambers, and each chamber is provided with an independent suction fan, so that the negative pressure distribution of different areas of the adsorption plate is uniform and controllable, the adsorption force of the adsorption hole on the pollen is ensured to be consistent through independent suction in different areas, and the uneven distribution or falling of the pollen caused by local overstrong or overweak adsorption is avoided, so that the sample representativeness and data reliability of subsequent detection are ensured, and the problem of uneven sample coverage caused by traditional single-point adsorption is overcome.

[0022] The vibration motor generates high-frequency micro-vibration, which is transmitted to the adsorption plate to force the pollen to move into the adsorption hole under the action of inertia and tightly fit. Through mechanical vibration, the pollen is quickly and uniformly embedded in the adsorption hole, manual intervention is reduced, and detection errors caused by pollen accumulation or loose distribution are avoided, so that the sample fixing efficiency and the standardization degree of the detection area are significantly improved.

[0023] The ultraviolet lamp excites the endogenous fluorescent substance in the pollen, the industrial camera synchronously photographs the fluorescent signal and quantifies the vitality level through image analysis algorithm, the non-destructive fluorescent detection technology is used to avoid the influence of the dyeing step on the pollen activity, the multi-camera interlaced layout is used to realize rapid scanning of the whole area, and the automatic image processing is combined to significantly improve the detection speed and objectivity, and overcome the defects of time-consuming, strong subjectivity and sample damage of the traditional dyeing method. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is the overall structure schematic diagram of the rice broad affinity pollen vitality rapid detection device in the embodiment of the present application;

[0025] Figure 2 is Figure 1 the internal structure schematic diagram of the rice broad affinity pollen vitality rapid detection device.

[0026] Fig. 1 is a detection table; 11, an adsorption plate; 12, a top plate; 121, a feeding hopper; 13, a cavity; 2, an adsorption mechanism; 21, a suction fan; 3, a vibration mechanism; 31, a vibration motor; 4, an air blowing mechanism; 41, an air blower; 42, an air expansion chamber; 43, a blowing nozzle; 5, a collection mechanism; 51, a collection hopper; 52, a collection bag; 6, a detection mechanism; 61, an ultraviolet lamp; 62, an industrial camera. Detailed Implementation

[0027] The following is in conjunction with the appendix Figures 1-2 This application will be described in further detail below.

[0028] This application discloses a rapid detection device for the viability of broad-compatibility rice pollen.

[0029] Reference Figure 1 and Figure 2 A rapid detection device for broad-compatibility pollen viability in rice includes a detection platform 1, an adsorption plate 11 with multiple adsorption holes on the detection platform 1, an adsorption mechanism 2 inside the detection platform 1 below the adsorption plate 11, a top plate 12 above the adsorption plate 11 with a feed hopper 121 on the top plate 12, a vibration mechanism 3 on the detection platform 1 for vibrating the adsorption plate 11, a blower 41 mechanism 4 on one side of the adsorption plate 11 for blowing away excess pollen that has not fallen into the adsorption holes, and a detection mechanism 6 for detecting pollen viability on the side of the top plate 12 near the adsorption plate 11.

[0030] Reference Figure 1 and Figure 2 The adsorption mechanism 2 includes multiple exhaust fans 21 installed in the detection platform 1. A cavity 13 is opened in the detection platform 1 above the adsorption plate 11. The cavity 13 is divided into multiple suction chambers of the same size, and each exhaust fan 21 is installed in the corresponding suction chamber.

[0031] The cavity 13 is divided into multiple uniform suction chambers, each equipped with an independent exhaust fan 21, ensuring uniform and controllable negative pressure distribution in different areas of the adsorption plate 11. This independent, zoned suction ensures consistent adsorption force of the adsorption pores on pollen, preventing uneven pollen distribution or shedding caused by excessively strong or weak local adsorption. This guarantees the representativeness of the samples and the reliability of the data in subsequent testing, overcoming the problem of uneven sample coverage caused by traditional single-point adsorption.

[0032] Vibration mechanism 3 includes a vibration motor 31 mounted on the testing table 1. The vibration motor 31 is used to cause the adsorption plate 11 to vibrate. (Refer to...) Figure 1 The vibration motor 31 generates high-frequency micro-amplitude vibrations, which are transmitted to the adsorption plate 11, forcing the pollen to move into the adsorption pores under inertia and adhere tightly. Mechanical vibration assists in the rapid and uniform embedding of pollen into the adsorption pores, reducing manual intervention and avoiding detection errors caused by pollen accumulation or loose distribution, thus significantly improving sample fixation efficiency and the standardization of the detection area.

[0033] Reference Figure 1 and Figure 2, the blower 41 mechanism includes the blower 41, the air expansion cavity 42 and the air blowing nozzle 43, the blower 41 is installed on the detection table 1, the air expansion cavity 42 is arranged on one side of the adsorption plate 11 and communicates with the blower 41, the air blowing nozzle 43 is provided with a plurality of air blowing nozzles 43, and each air blowing nozzle 43 is uniformly designed on the outer wall of the air expansion cavity 42 and communicates with the inner cavity of the air expansion cavity 42, and the other side of the adsorption plate 11 where the air blowing nozzle 43 is located is provided with a collection mechanism 5 for collecting the pollen blown off the adsorption plate 11.

[0034] The blower 41 inputs the airflow into the air expansion cavity 42, forms a directional and continuous airflow layer through the uniformly distributed air blowing nozzle 43, covers the surface of the adsorption plate 11 and sweeps the un-fixed pollen to the collection mechanism 5. The residual pollen is completely removed by the uniform airflow, which prevents it from interfering with the detection results, and the collection mechanism 5 recycles the swept material to avoid pollution, solving the problem of low efficiency and easy omission in manual cleaning in the traditional method, and ensuring the cleanliness and repeatability of the detection environment.

[0035] Referring to Figure 1 and Figure 2 , the collection mechanism 5 includes a collection bucket 51 and a collection bag 52, the collection bucket 51 is arranged obliquely on one side of the adsorption plate 11 and opposite to each air blowing nozzle 43, and the collection bag 52 is detachably arranged at the lower end of the collection bucket 51 and communicates with the collection bucket 51.

[0036] The pollen blown out by the air blowing nozzle 43 slides along the inclined surface of the collection bucket 51 to the collection bag 52 under the action of the airflow, and the collection bag 52 can be quickly detached and replaced. The impurities are efficiently recycled and centrally processed, avoiding the pollution of the environment by the pollen scattering, and the detachable design facilitates cleaning and maintenance, reduces operation cost and improves the stability of continuous operation of the device.

[0037] Referring to Figure 1 and Figure 2 , the detection mechanism 6 includes a plurality of ultraviolet lamps 61 and a plurality of industrial cameras 62, each ultraviolet lamp 61 is uniformly arranged on one side of the top plate 12 close to the adsorption plate 11, and each industrial camera 62 is arranged on one side of the top plate 12 close to the adsorption plate 11 and interposed between each ultraviolet lamp 61.

[0038] The ultraviolet lamp 61 excites the endogenous fluorescent substance in the pollen, and the industrial camera 62 synchronously photographs the fluorescent signal and quantifies the vitality level through image analysis algorithm. By using the non-destructive fluorescence detection technology, the influence of the dyeing step on the pollen activity is avoided, and the multi-camera interposed layout realizes the rapid scanning of the whole area, combined with the automatic image processing, which significantly improves the detection speed and objectivity, and overcomes the defects of time-consuming, strong subjectivity and sample destruction of the traditional dyeing method.

[0039] The implementation principle of the embodiment of the application is as follows: after the pollen falls into the adsorption plate 11 through the feeding hopper 121, the adsorption mechanism 2 fixes the pollen in the adsorption holes through negative pressure, the vibration mechanism 3 drives the adsorption plate 11 to vibrate, and the pollen is uniformly distributed and embedded in the adsorption holes, so that accumulation is avoided.

[0040] The blower 41 constructs 4 to blow the un-fixed excess pollen away from the adsorption plate 11, and eliminates background interference; the detection mechanism 6 performs rapid scanning analysis on the fixed pollen.

[0041] Through the synergistic control of adsorption, vibration, blowing and detection, the automatic positioning, impurity removal and high-throughput detection of the pollen are realized, the problems of low efficiency, large residual interference and dispersed detection process in the traditional method are solved, and the detection speed and accuracy are significantly improved.

[0042] The above are preferred embodiments of the application, and do not limit the protection scope of the application, so: any equivalent changes made according to the structure, shape, principle of the application should be covered within the protection scope of the application.

Claims

1. A rapid detection device for broad-compatibility rice pollen viability, characterized in that: The device includes a detection platform (1), on which an adsorption plate (11) is provided. The adsorption plate (11) has multiple adsorption holes. An adsorption mechanism (2) is provided in the detection platform (1) below the adsorption plate (11). A top plate (12) is provided above the adsorption plate (11). A feed hopper (121) is provided on the top plate (12). A vibration mechanism (3) is provided on the detection platform (1) to make the adsorption plate (11) vibrate. A blower (41) mechanism (4) is also provided on one side of the adsorption plate (11) to blow away excess pollen on the adsorption plate (11) that has not fallen into the adsorption holes. A detection mechanism (6) for detecting pollen viability is provided on the side of the top plate (12) near the adsorption plate (11).

2. The rapid detection device for broad-compatibility pollen viability in rice according to claim 1, characterized in that: The adsorption mechanism (2) includes multiple exhaust fans (21) disposed in the detection platform (1). A cavity (13) is opened in the detection platform (1) above the adsorption plate (11). The cavity (13) is divided into multiple suction chambers of the same size. Each exhaust fan (21) is disposed in the corresponding suction chamber.

3. The rapid detection device for rice broad-compatibility pollen viability according to claim 2, characterized in that: The vibration mechanism (3) includes a vibration motor (31) disposed on the detection table (1), and the vibration motor (31) is capable of causing the adsorption plate (11) to vibrate.

4. The rapid detection device for broad-compatibility pollen viability in rice according to claim 1, characterized in that: The blower (41) mechanism includes a blower (41), an air expansion chamber (42), and an air nozzle (43). The blower (41) is installed on the testing platform (1). The air expansion chamber (42) is located on one side of the adsorption plate (11) and is connected to the blower (41). There are multiple air nozzles (43), and each air nozzle (43) is evenly spaced on the outer wall of the air expansion chamber (42) and is connected to the inner cavity of the air expansion chamber (42). On the other side of the adsorption plate (11) where the air nozzle (43) is located, there is a collection mechanism (5) for collecting the pollen blown down from the adsorption plate (11).

5. The rapid detection device for broad-compatibility pollen viability in rice according to claim 4, characterized in that: The collection mechanism (5) includes a collection hopper (51) and a collection bag (52). The collection hopper (51) is inclinedly disposed on one side of the adsorption plate (11) and is directly opposite to each of the air nozzles (43). The collection bag (52) is detachably disposed at the lower end of the collection hopper (51) and is connected to the collection hopper (51).

6. The rapid detection device for rice broad-compatibility pollen viability according to claim 1, characterized in that: The detection mechanism (6) includes an ultraviolet lamp (61) and an industrial camera (62). Multiple ultraviolet lamps (61) are provided, and each ultraviolet lamp (61) is evenly arranged on the side of the top plate (12) near the adsorption plate (11). Multiple industrial cameras (62) are provided, and each industrial camera (62) is arranged on the side of the top plate (12) near the adsorption plate (11) and is interspersed among the ultraviolet lamps (61).

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