Sterile microsphere mass production platform device

Abstract

The utility model provides a sterile microsphere mass production platform device. The sterile microsphere mass production platform device comprises a rack with a closed space, and a liquid storage module, a liquid separation module, chips, a container and a stirrer which are mounted in the closed space of the rack, a plurality of groups of chips are arranged, and each group comprises a plurality of chips; the liquid storage module is communicated with the plurality of chips through the liquid separation module, one container is correspondingly arranged below each group of chips, and the stirrer is arranged at the bottom of each container. According to the utility model, various modules for preparing monodisperse liquid drops are arranged in the closed space of the rack, the whole process is kept in a sterile state, and the liquid drops are prepared through a plurality of chips and then solidified through the stirrer, so that the preparation efficiency of the liquid drops is greatly improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microsphere mass production especially relates to a sterile microsphere mass production platform device. BACKGROUND

[0002] Material microsphere (monodisperse micro-nano SiO2) has small size effect, surface effect, excellent optical, electrical, magnetic, mechanical, mechanical and other properties, and is widely used in modern industrial production. It is also an important basis for the development of future high-tech and emerging industries, new medicines, flat panel displays, food safety testing, chemical industry and other industries cannot do without advanced micro-nano microsphere materials.

[0003] At present, in the preparation process of material microspheres, the most widely used methods are emulsification evaporation method, phase separation method, spray drying method, hot melt extrusion method and the like. However, most of the technologies have certain disadvantages, and the uniformity of the particle size of the slow-release microspheres is poor due to the defects of the manufacturing process, the residual of the internal toxic solvent, the low drug loading rate and encapsulation rate, and the microsphere adhesion, which cannot achieve the best microsphere preparation effect.

[0004] Under the comparison of the application of various preparation methods, microfluidic technology shows unique advantages in microsphere preparation. The technology can produce high repeatability and controllable regular uniform droplets through the immiscible multi-phase fluid in the microchannel. The produced droplets can be used as templates to prepare spherical products with regular shape, uniform particle size distribution and high coating efficiency. The application of microfluidic technology in the preparation of material microspheres will be beneficial to the high-throughput preparation of microspheres with high quality, uniform structure and morphology and narrow particle size distribution range.

[0005] The existing preparation process, such as the preparation method and micro-reaction system of SiO2 micro-nano balls disclosed in patent publication No. CN102120585A, includes a unit reaction device, which is composed of a feeding device, a reinforced micro-reaction device and a storage tank. The microspheres are produced in an open space, which has a series of problems, such as microorganisms in the air, pyrogen, human interference in gas circulation, low efficiency and the like. UTILITY MODEL CONTENT

[0006] The utility model aims at providing a sterile microsphere mass production platform device to solve the above technical problems.

[0007] The technical scheme of the utility model is as follows: a sterile microsphere mass production platform device, which comprises a rack with a closed space and a liquid storage module, a liquid distribution module, a chip, a receiving container and a stirrer installed in the closed space of the rack, the chip is provided with multiple groups, each group has multiple chips, the liquid storage module is communicated with multiple chips through the liquid distribution module, one receiving container is arranged below each group of chips, and the bottom of the receiving container is provided with the stirrer.

[0008] In the above scheme, various modules for preparing monodisperse droplets are arranged in the closed space of the rack, so that the droplet preparation and solidification processes are carried out in a sterile state, the microorganisms in the air, the pyrogen and the human interference in the gas circulation are avoided, and the preparation efficiency of the droplets is greatly improved.

[0009] Preferably, the sterile microsphere mass production platform device further comprises a lifting mechanism arranged in the rack, and a plurality of the chips are hung on the lifting mechanism through clamps, and the lifting mechanism lifts the chips relative to the container. The lifting mechanism is used for adjusting the height position of the chip to adjust the distance between the chip and the container.

[0010] Preferably, the liquid storage module is provided with at least two, wherein at least one is installed with an aqueous phase solution, and at least one is installed with an oil phase module, and each of the liquid storage modules is connected with the chip through the liquid distribution module.

[0011] Preferably, the sterile microsphere mass production platform device further comprises a liquid supply module arranged in the closed space of the rack, one end of the liquid supply module is provided with a liquid supply inlet, and the other end of the liquid supply module is in communication with the liquid storage module.

[0012] Preferably, the sterile microsphere mass production platform device further comprises a monitoring module arranged in the closed space of the rack, a camera electrically connected with the monitoring module, a horizontal movement mechanism for driving the camera to displace between the plurality of chips, and a control module for sending action instructions to the horizontal movement mechanism.

[0013] Preferably, an air filter is arranged on the rack, one end of the air filter is provided with an air inlet, and the other end of the air filter is in communication with the inside of the rack.

[0014] Preferably, a suction device is arranged on the top of the rack, one end of the suction device is provided with an air outlet, and the other end of the suction device is in communication with the inside of the rack.

[0015] Preferably, a glove box and a movable door are arranged on the side wall of the rack, the glove box is arranged corresponding to the container, and the movable door is located above the glove box.

[0016] Preferably, a material box is arranged at one end of the rack, and the material box is in communication with the inside of the rack.

[0017] Preferably, an ultraviolet sterilization system is arranged on the inner top surface of the rack.

[0018] Compared with the related art, the device has the advantages that:

[0019] I. The sterile microsphere mass production platform device sets various modules for preparing monodisperse droplets in the closed space of the rack, ensures that the droplet preparation and solidification process are carried out in a sterile state throughout the process, avoids microorganisms in the air, heat sources, and human interference in the gas circulation, and greatly improves the droplet preparation efficiency;

[0020] II. A monitoring module and a camera are additionally arranged, and the camera is driven to move horizontally between a plurality of chips through a horizontal movement mechanism, so that the production state is monitored, and the stability of the platform device is ensured;

[0021] III. The sterile microsphere mass production platform device prepares droplets in the closed rack, guarantees a sterile working environment, and avoids contamination of exogenous microorganisms in the overall production process of the microspheres, so that the high-quality production of medical material microspheres is met;

[0022] IV. An ultraviolet sterilization system is arranged in the rack to further guarantee the sterile working environment;

[0023] V. A material box with an independent storage space is arranged beside the rack, which is used for storing related instruments and solutions to be used, improves the convenience of the operation of the platform device, and is helpful for the operator to operate in the rack;

[0024] VI. The sterile microsphere mass production platform device relies on a suction device and / or an air filter to filter the gas entering the inside of the platform multiple times to remove microorganisms in the gas. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 A front view structural schematic view of the sterile microsphere mass production platform device provided by the present application is provided.

[0026] Figure 2 A rear view structural schematic view of the sterile microsphere mass production platform device provided by the present application is provided.

[0027] Figure 3 An internal structure schematic view (I) of the sterile microsphere mass production platform device provided by the present application is provided.

[0028] Figure 4 An internal structure schematic view (II) of the sterile microsphere mass production platform device provided by the present application is provided.

[0029] Figure 5 A mounting structure schematic view of a liquid supply module, a liquid distribution module, a chip and a receiving container is provided.

[0030] In the drawing: 1, liquid supply module; 2, liquid storage module; 3, liquid distribution module; 4, chip; 5, pressure control center; 6, control module; 7, monitoring module; 8, receiving container; 9, stirrer; 10, suction device; 11, air filter; 12, material box; 13, lifting mechanism; 131, motor; 132, first gear; 133, belt; 134, lifting frame; 135, second gear; 136, guide rod; 137, mounting seat; 138, transmission rod; 14, horizontal moving mechanism; 15, ultraviolet sterilization system; 16, power supply module; 17, glove box; 18, camera; 19, movable door; 20, wrench; 21, rack. DETAILED DESCRIPTION

[0031] The utility model will be described in detail below with reference to the drawings and in combination with the embodiments. It should be noted that the embodiments in the utility model and the features in the embodiments can be combined with each other without conflict. For the sake of description, if "up", "down", "left" and "right" appear in the following text, they only mean consistent with the up, down, left and right directions of the drawing itself, and do not limit the structure.

[0032] As shown in Figure 1 , Figure 3 , the sterile microsphere mass production platform device provided by the embodiment includes a liquid supply module 1, a liquid storage module 2, a liquid distribution module 3, a chip 4, a pressure control center 5, a control module 6, a monitoring module 7, a receiving container 8, a stirrer 9, a suction device 10, an air filter 11, a material box 12, a lifting mechanism 13, a horizontal moving mechanism 14, an ultraviolet sterilization system 15, a power supply module 16, a glove box 17, a camera 18, a movable door 19, a wrench 20 and a rack 21.

[0033] As shown in Figure 1 , Figure 2 , the rack 21 is a box-shaped structure, the bottom is a frame body, and the upper part is a box body closed on all sides. The box body has a plurality of compartments inside to install the modules for each work. The skeleton of the rack 21 is stainless steel, and the side wall is thickened light-transmitting glass. The rack 21 has a length direction X, a width direction Y and a height direction Z. The inside of the box body is a sterile working cavity.

[0034] The liquid supply module 1 is installed at one end of the length direction X inside the box body and is provided with two. The liquid storage module 2 is installed at the other end of the length direction X in the box body through a mounting bracket. One end of the liquid supply module 1 is provided with a liquid supply inlet, and the liquid supply inlet is in communication with the liquid supply equipment outside the platform device. The other end of the liquid supply module 1 is in communication with the liquid storage module 2 through a pipeline. The liquid supply module 1 is selected as a constant pressure pump for liquid supply, and in its working state, the solution prepared in the external liquid supply equipment is transferred to the liquid storage module 2 through the pipeline.

[0035] The liquid storage module 2 is two large-capacity stainless steel tanks, respectively storing water phase and oil phase solutions. As shown in Figure 5 The bottom of the two liquid storage modules 2 is provided with a liquid distribution module 3. The liquid distribution module 3 is controlled by a plurality of independent stainless steel pipes and independent valves. The number of stainless steel pipes corresponds to the number of chips 4.

[0036] The lifting mechanism 13 is installed in the box of the rack 21 and located beside the Y direction of the liquid distribution module 2. The lifting mechanism 13 includes a motor 131, a first gear 132, a belt 133, a lifting frame 134, a second gear 135, a guide rod 136, a mounting seat 137 and a transmission rod 138.

[0037] The mounting seat 137 is provided with two mounting seats 137 in the rack 21, and the two mounting seats 137 are spaced apart in the X direction. The motor 131 is installed at the lower end of one of the mounting seats 137. The transmission rod 138 is rotatably provided at the upper end of the mounting seat 137 through a bearing. The first gear 132 is installed on the motor shaft of the motor 131. The other mounting seat 137 is rotatably provided with a first gear 132, and the two first gears 132 are in corresponding positions. Two second gears 135 are installed on the transmission rod 138, and the second gears 135 correspond to the first gears 132 and are connected by the belt 133. A guide rod 136 is provided in each mounting seat 137 along the Z direction. The two ends of the lifting frame 134 are slidably connected with the guide rods 136. One side of the belt 133 is connected with the lifting frame 134. The belt 133 is driven to rotate by the motor 131 to make the lifting frame 134 ascend and descend.

[0038] A clamp is provided on the side surface of the lifting frame 134. In this embodiment, the clamp is a screw. The chip 4 is a PDMS (Polydimethylsiloxane) chip, which is provided with a plurality of groups, each group having a plurality of chips. Each chip 4 is provided with a hanging hole at the top and is hung on the screw to make the lower part of the chip 4 hang. The chip 4 ascends and descends with the lifting frame 134. The clamp can also be a clip to clamp the chip 4.

[0039] As shown in Figure 3 , Figure 5 The liquid storage module 2 and the plurality of chips 4 are communicated through the liquid distribution module 3. Under the action of the operator, the liquid distribution module 3 can realize the liquid distribution flow, control the independent water and oil flow paths to flow into the chip 4 through the stainless steel pipes of the liquid distribution module 3.

[0040] The cabinet side wall of the rack 21 is provided with a pressure control center 5, a control module 6 and a monitoring module 7. The rack body of the rack 21 is provided with a power supply module 16. The pressure control center 5, the monitoring module 7 and the power supply module 16 are electrically connected with the control module 6 and an external display panel. By setting a pressure parameter on the display panel, the pressure parameter is converted into a liquid supply speed by the control module 6 and sent to the pressure control center 5 to control the constant pressure pump of the liquid supply module 1, so that the constant pressure pump operates at the set liquid supply speed to ensure the pressure parameter during chip operation. The control module 6 also allocates and controls the lifting mechanism 13 and the transverse moving mechanism 14, which can also be set on the display panel.

[0041] As shown in Figure 3 , one of the containers 8 is arranged below each group of chips 4, and the bottom of the container 8 is provided with the stirrer 9. The container 8 is a glass container with a volume of 2L, which is used to collect the prepared droplets. The stirrer 9 is a magnetic stirrer, which is used to solidify and extract the droplets in the container 8, so as to realize stable solidification of the droplets.

[0042] The top of the cabinet of the rack 21 is provided with a suction device 10. One end of the suction device 10 is provided with an air outlet, and the other end of the suction device 10 is in communication with the inside of the rack 21. The suction device 10 is a gas outlet passage, and the air outlet thereof is in communication with an external air blower, so as to realize suction of the gas in the cabinet by generating negative pressure.

[0043] The bottom of the cabinet of the rack 21 is provided with an air filter 11. One end of the air filter 11 is provided with an air inlet, and the other end of the air filter 11 is in communication with the inside of the rack 21. The air filter 11 is a gas inlet passage, and a plurality of filter screens are arranged in the passage. The air inlet of the air filter 11 is connected with an external air purifier, so as to perform multiple filtering on the gas entering the platform device, remove microorganisms in the gas, and ensure the quality of the flowing gas.

[0044] The suction device 10 is used in combination with the air filter 11 to realize air circulation in the platform, and remove volatile gas generated by solidification of the droplets in the platform device.

[0045] As shown in Figure 1 , Figure 2 , one end of the rack 21 is provided with a material box 12, and the material box 12 is in communication with the inside of the rack 21. The material box 12 is used to place standby chips 4 and solutions. The solution is used to pour into the container 8. One side of the material box 12 is provided with an openable and closable door.

[0046] As shown in Figure 1 , Figure 4As shown, the Y direction of the lifting mechanism 13 is provided with a horizontal movement mechanism 14. The horizontal movement mechanism 14 is a slide rail, a slide block and a motor for driving the displacement of the slide block. The slide block is displaced along the X direction. The slide block is provided with a camera 18. The camera 18 is electrically connected with the monitoring module 7.

[0047] The lifting mechanism 13 is used for adjusting the height position of the chip 4, so as to adjust the distance between the chip 4 and the container 8. When the container 8 is full of liquid drops and needs to be replaced, the chip 4 is lifted. The horizontal movement mechanism 14 is used for adjusting the position of the camera 18, so as to monitor the state of the chip 4 at different positions and display on the display panel.

[0048] As shown, Figure 2 As shown, the inner top surface of the box body is provided with an ultraviolet sterilization system 15. Before the platform device is operated, the ultraviolet sterilization system 15 is turned on to maintain the sterile state inside. The power supply module 16 is used for supplying power to the entire platform device.

[0049] As shown, Figure 1 、 Figure 2 As shown, the Y direction of the two side walls of the rack 21 is provided with a glove box 17 and a movable door 19. The glove box 17 is arranged corresponding to the container 8, and the movable door 19 is located above the glove box 17. The movable door 19 is hinged to the skeleton of the box body and is limited in the opening or closing position by the air supporting rod. The movable door 19 is provided with a wrench 20. The wrench 20 is turned to be unlocked or locked. The glove box 17 is convenient for the operator to put his hand into the box body to operate. The connection between the movable door 19 and the skeleton is sealed with a silica gel strip to realize the sealing of the internal space of the box body and avoid the pollution of external microorganisms.

[0050] In use, solutions for dispersed phase and continuous phase in the microfluidic system are prepared. The solution for dispersed phase is a solution of material required for microsphere preparation, and the solution for continuous phase is an aqueous solution containing a surfactant, which is used for stable shearing and receiving solidification of liquid drops. The power supply module 16 and the liquid supply module 1 are turned on. The prepared two solutions are sequentially pumped into two groups of liquid storage modules 2 through the liquid supply module 1 and the pipeline, respectively, and the liquid supply module 1 is stopped. The operator puts his hand into the glove box 17 and performs the following operations: the chip 4 is vertically placed on the lifting frame 134. The stainless steel pipeline on the dispensing module 3 is connected with the interface of the corresponding chip 4 (the shape of the stainless steel pipeline is customized according to the position of the chip 4 to be connected, and the pipeline end is provided with an interface and is installed in a plug-in manner). The solution is taken from the material box 12 and poured into the container 8 (to half of the container 8). The operator withdraws his hand.

[0051] The working parameters are set on the display panel. The control module 6 sends instructions to start the lifting mechanism 13 to lower the chip 4 to the set working height, and to start the horizontal movement mechanism 14 to move the camera 18 to the initial position. The movable doors 19 are closed.

[0052] The suction device 10 and the air filter 11 are turned on to circulate the gas inside the box. The ultraviolet sterilization system 15 is turned on to perform overall sterilization on the platform, and runs continuously for 1-2 hours.

[0053] After the ultraviolet sterilization is completed, the lifting mechanism 13 is started to immerse the chip 4 in the container 8. The liquid supply module 1 is started, and the solution in the liquid storage module 2 enters the chip 4 through the liquid distribution module 3 to prepare droplets (prepare microspheres). The prepared microsphere droplets are dropped into the container 8 below. The magnetic stirrer 9 is started to continuously solidify the microspheres in the container 8. During the preparation process, the horizontal movement mechanism 14 is started to drive the camera 18 to capture the microsphere preparation process between multiple chips 4. Real-time image data is transmitted to the monitoring module 7 and the control module 6, and displayed on the display panel.

[0054] After a period of preparation, the liquid supply module 1 and the horizontal movement mechanism 14 are turned off, and the suction device 10, the air filter 11 and the stirrer 9 are continuously operated for a period of time to continuously solidify the microspheres in the container 8.

[0055] When the container 8 is full of microspheres, the operator takes out the container 8 and places it on the material box 12, and replaces a new container 8. The liquid storage module 2 needs to be disassembled and washed after use.

[0056] The above only describes the embodiments of the present application, and does not limit the patent range of the present application. Any equivalent structure or equivalent process conversion, or direct or indirect application in other related technical fields, is also included in the patent protection range of the present application.

Claims

1. A sterile microsphere mass production platform device, characterized by, The rack (21) with a closed space and the liquid storage module (2), the liquid distribution module (3), the chip (4), the container (8) and the stirrer (9) installed in the closed space of the rack (21), the chip (4) is provided with multiple groups, each group has multiple; the liquid storage module (2) and multiple chip (4) are communicated through the liquid distribution module (3), and one container (8) is arranged below each group of chip (4), and the bottom of the container (8) is provided with the stirrer (9).

2. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, It also includes a lifting mechanism (13) arranged in the closed space of the rack (21), and multiple chip (4) is hung on the lifting mechanism (13) through a clamp, and the lifting mechanism (13) makes the chip (4) lift relative to the container (8).

3. The sterile microsphere manufacturing platform device of claim 1, wherein, The liquid storage module (2) is provided with at least two, wherein at least one is installed with water phase solution, and at least one is installed with oil phase module, and each liquid storage module (2) is connected with the chip (4) through the liquid distribution module (3).

4. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, It also includes a liquid supply module (1) arranged in the closed space of the rack (21), one end of the liquid supply module (1) is provided with a liquid inlet, and the other end of the liquid supply module (1) is communicated with the liquid storage module (2).

5. The sterile microsphere manufacturing platform device of claim 1, wherein, It also includes a monitoring module (7) arranged in the closed space of the rack (21), a camera (18) electrically connected with the monitoring module (7), a horizontal movement mechanism (14) for driving the camera (18) to displace between multiple chip (4), and a control module (6) for sending action instruction to the horizontal movement mechanism (14).

6. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, The rack (21) is provided with an air filter (11), one end of the air filter (11) is provided with an air inlet, and the other end of the air filter (11) is communicated with the inside of the rack (21).

7. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, The top of the rack (21) is provided with a suction device (10), one end of the suction device (10) is provided with an air outlet, and the other end of the suction device (10) is communicated with the inside of the rack (21).

8. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, The side wall of the rack (21) is provided with a glove box (17) and a movable door (19), the glove box (17) is arranged corresponding to the container (8), and the movable door (19) is located above the glove box (17).

9. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, One end of the rack (21) is provided with a material box (12), and the material box (12) is communicated with the inside of the rack (21).

10. The sterile microsphere high volume manufacturing platform device of claim 1, wherein, The inner top surface of the rack (21) is provided with an ultraviolet sterilization system (15).

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