Nucleic acid extraction and purification device

Abstract

The utility model relates to the field of nucleic acid purification, and discloses a nucleic acid extraction and purification device which comprises a purification machine, the top of the purification machine is electrically connected with a display screen, the bottom of the purification machine is fixedly connected with a plurality of supporting pads, one side of the purification machine is rotatably connected with a rotating shaft, and a plurality of liquid guide assemblies are arranged in the purification machine. A box door is rotatably connected to the outer wall of the rotating shaft, an observation window is formed in the box door, a handle is fixedly connected to one side of the box door, a plurality of fixing columns are fixedly connected to one side of the purifier, and a fixing assembly is arranged in each fixing column. According to the utility model, firstly, the handle is held to lift the box door upwards, and when the box door is completely closed, the fixing pin is popped out under the action of the spring I, so that the effect of easily opening the box door is achieved, and the problem of solution purification failure caused by complex opening of the box door due to failure of internal equipment is avoided; therefore, the high efficiency of the nucleic acid extraction and purification device is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of nucleic acid purification, especially to a nucleic acid extraction and purification device. BACKGROUND

[0002] Nucleic acid purification refers to the process of separating and purifying nucleic acid from biological samples, removing impurities such as proteins and polysaccharides to obtain high-purity nucleic acid. The use of nucleic acid extraction and purification devices can effectively remove impurities through a series of processes such as lysis and adsorption, ensuring the purity and quality of nucleic acid and meeting the stringent requirements of subsequent experiments such as PCR amplification. On the other hand, with optimized extraction methods, nucleic acid extraction efficiency and yield can be improved in a short time. In addition, such devices can achieve automation and high-throughput operation, processing multiple samples at a time, meeting the needs of large-scale genetic testing scenarios, reducing errors and cross-contamination risks caused by manual operation, and reducing human factor interference through standardized operation. With a closed reaction system and pollution control measures, nucleic acid is protected from external contamination.

[0003] The existing nucleic acid extraction and purification device mainly operates based on three principles: silica gel membrane adsorption, magnetic bead separation, and centrifugal column chromatography. The silica gel membrane adsorption method first lyses the sample to release nucleic acid, which is then adsorbed onto the silica gel membrane in a high-salt low-pH environment. After washing the impurities with a low-salt high-pH washing solution, the nucleic acid is eluted with a low-salt buffer or deionized water. The magnetic bead separation method involves adding magnetic nanoparticles with special functional groups after lysing the sample, allowing the nucleic acid to bind specifically to them. The magnetic beads and impurities are separated using an external magnetic field, and the nucleic acid is obtained after multiple washes and desorption. The centrifugal column chromatography method involves loading the sample into a centrifugal column filled with special filler after lysing the sample. The small molecular impurities enter the filler pores under the action of centrifugal force, while the nucleic acid is blocked between the particles. Finally, the purified nucleic acid is obtained by washing with a buffer solution and eluting with an eluent.

[0004] In the actual use scenario of the nucleic acid extraction and purification device, when a key component inside the device such as the magnetic separation module, temperature control module, or liquid handling system fails, the staff needs to quickly open the door to repair and debug the device. However, the current nucleic acid extraction and purification device has a complex door opening structure design, with tedious operation steps involving multiple locking and unlocking procedures, making it difficult for staff to open the door in the first time to handle the problem. This not only wastes valuable time, but also forces the ongoing nucleic acid solution purification process to be interrupted, causing the sample to deteriorate or contaminate during the waiting period, ultimately leading to solution purification failure, severely affecting the efficient operation of the nucleic acid extraction and purification device. SUMMARY

[0005] To address the above problems, the present utility model provides a nucleic acid extraction and purification device to improve the problem of complex door opening caused by internal device failure during device use, leading to solution purification failure.

[0006] In order to achieve the above object, the utility model discloses the following technical scheme: a nucleic acid extraction and purification device, including the purification machine, the purification machine top electric connection has display screen, the purification machine bottom fixed connection has a plurality of support pad, the purification machine one side rotatable connection has the pivot, the purification machine is internally provided with a plurality of liquid guide components, the pivot outer wall rotatable connection has the box door, the box door is internally provided with the observation window, the box door one side fixed connection has the handle, the purification machine one side fixed connection has a plurality of fixed column, each fixed column is internally provided with fixed component all,

[0007] Each fixed component all includes fixed pin, limit board and spring one, each fixed pin outer wall all slidingly connected in the fixed column, each limit board inner wall all fixed connection in the fixed pin outer wall, each spring one all is arranged in the fixed column, each spring one one end all fixed connection in the fixed column, and each spring one the other end all fixed connection in the fixed pin top.

[0008] As a further description of the above technical scheme:

[0009] Each liquid guide component all includes moving rod, a plurality of liquid guide tubes, connecting rod and a plurality of soft hoses, each moving rod one end all slidingly connected in the purification machine, each liquid guide tube top all fixed connection in moving rod bottom, each connecting rod top all fixed connection in liquid guide tube bottom, and each soft hose top all fixed connection in connecting rod bottom.

[0010] As a further description of the above technical scheme:

[0011] The purification machine is internally slidably connected with the pull block, and the pull block one side fixed connection has the handle.

[0012] As a further description of the above technical scheme:

[0013] The pull block is internally slidably connected with a plurality of sliding blocks, and each sliding block top all fixed connection has the moving block.

[0014] As a further description of the above technical scheme:

[0015] Each moving block one side all fixed connection has a plurality of connecting columns, and each moving block bottom all slidingly connected in the pull block top.

[0016] As a further description of the above technical scheme:

[0017] Each sliding block one side all is provided with spring two, and each spring two one end all fixed connection in the sliding block one side, and each spring two the other end all fixed connection in the pull block.

[0018] As a further description of the above technical solutions:

[0019] The top of the pulling block is slidably connected with a test tube box, and a plurality of reagent tubes are slidably connected inside the test tube box.

[0020] As a further description of the above technical solutions:

[0021] The connecting columns are arranged in parallel array on one side of the moving block, and the outer wall of each connecting column is slidably connected inside the test tube box.

[0022] The utility model has the advantages of the following:

[0023] 1. In the utility model, first, press down the fixed pin to make it retract into the fixed column, then hold the handle to open the box door by the pivot, then operate the internal structure, hold the handle to lift the box door up after operation, make the hole at the top of the box door slide on the outer wall of the fixed column, and then make the fixed pin retract into the fixed column. When the box door is completely closed, the fixed pin is ejected under the action of the spring I to fix the box door, so that the box door can be easily opened to operate the internal equipment during equipment use, avoiding the problem of solution purification failure caused by the complexity of opening the box door when the internal equipment fails during equipment use, thereby improving the efficiency of the nucleic acid extraction and purification device.

[0024] 2. In the utility model, first, hold the handle to pull out the pulling block, then hold the moving block to pull it outwards, so that the connecting column is pulled out from the inside of the test tube box, then take out the test tube box, then put the reagent tube to be treated into the test tube box, then put the test tube box back into the pulling block, and at the same time, loosen the moving block to make it shrink inwards under the action of the spring II to fix the test tube box, so that the test tube box can be fixed in the purification machine during equipment use, so that the test tube box is not easy to shake during purification, avoiding the problem that the purification process is affected by the shaking of the test tube box during equipment use, thereby improving the practicability of the nucleic acid extraction and purification device. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 A perspective view of the nucleic acid extraction and purification device is provided for the utility model;

[0026] Figure 2 A box door structure schematic view of the nucleic acid extraction and purification device is provided for the utility model;

[0027] Figure 3 For Figure 2 A local enlarged structure schematic view of A in the middle;

[0028] Figure 4The utility model provides a kind of nucleic acid extraction and purification device's purification machine internal structure schematic view is proposed.

[0029] Figure 5 For Figure 4 Local amplification structure schematic view of B in middle.

[0030] Legend:

[0031] 1, purification machine;2, display screen;3, support pad;4, shaft;5, box door;6, observation window;7, handle;8, fixed column;9, fixed pin;10, limit plate;11, spring one;12, moving rod;13, liquid guide pipe;14, connecting rod;15, soft rubber tube;16, pull block;17, handle;18, sliding block;19, moving block;20, connecting column;21, spring two;22, test tube box;23, reagent tube. Specific implementation

[0032] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0033] With reference Figures 1-3 The utility model provides an embodiment: a kind of nucleic acid extraction and purification device, including purification machine 1, display screen 2 is electrically connected in the top of purification machine 1, for showing internal data, the bottom of purification machine 1 is fixedly connected with multiple support pads 3, for supporting equipment, one side of purification machine 1 is rotatably connected with shaft 4, for rotating box door 5, multiple liquid guide components are provided in the inside of purification machine 1, for carrying out purification operation, shaft 4 outer wall is rotatably connected with box door 5, for sealing equipment, observation window 6 is arranged in the inside of box door 5, for observing equipment internal operating condition, one side of box door 5 is fixedly connected with handle 7, for pulling box door 5, one side of purification machine 1 is fixedly connected with multiple fixed columns 8, for connecting box door 5, each fixed column 8 is provided with fixed component in the inside, for fixing box door 5;

[0034] Each fixing assembly comprises a fixing pin 9, a limiting plate 10 and a spring 11, the outer wall of each fixing pin 9 is slidably connected in the inner part of the fixing column 8, for fixing the box door 5, the inner wall of each limiting plate 10 is fixedly connected to the outer wall of the fixing pin 9, for limiting the moving range of the fixing pin 9, each spring 11 is arranged in the inner part of the fixing column 8, for providing elastic force for the fixing pin 9, one end of each spring 11 is fixedly connected to the inner part of the fixing column 8, and the other end of each spring 11 is fixedly connected to the top of the fixing pin 9, each liquid guiding assembly comprises a moving rod 12, a plurality of liquid guiding pipes 13, a connecting rod 14 and a plurality of soft rubber pipes 15, one end of each moving rod 12 is slidably connected in the inner part of the purifier 1, for moving the liquid guiding pipe 13, the top end of each liquid guiding pipe 13 is fixedly connected to the bottom of the moving rod 12, for extracting or guiding out the solution, the top of each connecting rod 14 is fixedly connected to the bottom end of the liquid guiding pipe 13, for connecting the liquid guiding pipe 13 and the soft rubber pipe 15, and the top end of each soft rubber pipe 15 is fixedly connected to the bottom of the connecting rod 14, for contacting with the solution.

[0035] Specifically, when the internal equipment of the purifier 1 needs to be overhauled, debugged or the like, the operator needs to press the fixing pin 9 first, so that it slowly shrinks into the inner part of the fixing column 8, then the operator holds the handle 7 to open the box door 5 by the rotating shaft 4, at this time, the box door 5 is stably rotated under the driving of the rotating shaft 4, and the internal equipment structure is exposed, so that the operator can smoothly operate it, after the operation is completed, the operator holds the handle 7 again and lifts the box door 5 upward, the hole in the top of the box door 5 slowly slides along the outer wall of the fixing column 8, in this process, the fixing pin 9 gradually shrinks into the inner part of the fixing column 8, when the box door 5 is completely closed in place, the fixing pin 9 is popped out under the elastic force of the spring 11, so as to firmly fix the box door 5, and ensure the safety and stability of the equipment in the subsequent operation.

[0036] Referring to Figure 4 and Figure 5The pulling block 16 is internally slidably connected with the purification machine 1, is used for moving the test tube box 22, one side of the pulling block 16 is fixedly connected with the handle 17, is used for pulling the pulling block 16, the pulling block 16 is internally slidably connected with a plurality of sliding blocks 18, is used for connecting the moving block 19, the top of each sliding block 18 is fixedly connected with the moving block 19, is used for being connected with the connecting column 20, one side of each moving block 19 is fixedly connected with a plurality of connecting columns 20, is used for fixing the test tube box 22, the bottom of each moving block 19 is slidably connected at the top of the pulling block 16, one side of each sliding block 18 is provided with the spring two 21, provides elastic force for the movement of the moving block 19, one end of each spring two 21 is fixedly connected at one side of the sliding block 18, the other end of each spring two 21 is fixedly connected in the pulling block 16, the top of the pulling block 16 is slidably connected with the test tube box 22, is used for loading reagent tube 23, the inside of the test tube box 22 is slidably connected with a plurality of reagent tubes 23, is used for containing reagent, the connecting column 20 is arranged in parallel array between the moving block 19 side, the outer wall of each connecting column 20 is slidably connected in the test tube box 22.

[0037] Specifically, in the preparation link of nucleic acid extraction and purification, when the reagent tube 23 needs to be placed in the test tube box 22, the staff first holds the handle 17 and pulls outwards, the pulling block 16 is smoothly pulled out, then the staff holds the moving block 19 and pulls it outwards, with the movement of the moving block 19, the connecting column 20 slowly comes out from the inside of the test tube box 22, at this time, the staff takes out the test tube box 22, then the staff puts the reagent tube 23 to be processed into the corresponding slot in the test tube box 22 in order, after the reagent tube 23 is placed, the staff puts the test tube box 22 back to the designated position in the pulling block 16, at the same time, the staff releases the control to the moving block 19, under the elastic force of the spring two 21, the moving block 19 rapidly shrinks inwards, the connecting column 20 is also quickly inserted into the corresponding clamping groove of the test tube box 22, and the test tube box 22 is stably fixed, and the installation of the reagent tube 23 is completed.

[0038] Working principle: in the process of using nucleic acid extraction and purification device, first press the fixed pin 9 to make it retract into the fixed column 8 inside, then hold the handle 7 to open the box door 5 by using the shaft 4, then hold the pull handle 17 to pull out the pull block 16, then hold the moving block 19 to pull it outwards, so that the connecting column 20 is pulled out from the inside of the test tube box 22, so as to take out the test tube box 22, then put the reagent tube 23 which needs to be processed into the test tube box 22, then put the test tube box 22 back into the pull block 16, at the same time, loosen the moving block 19 to make it shrink under the action of spring 21, so that the connecting column 20 is reinserted into the test tube box 22, to fix the test tube box 22, so as to complete the installation of the reagent tube 23, then hold the handle 7 to lift the box door 5 upwards, so that the hole on the top of the box door 5 slides on the outer wall of the fixed column 8, so that the fixed pin 9 retracts into the fixed column 8, when the box door 5 is closed, the fixed pin 9 is pulled out under the action of spring 11 to fix the box door 5, then start the equipment to purify the solution in the reagent tube 23.

[0039] Finally, it should be pointed out that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the foregoing detailed description of the present application, for the skilled in the art, it still can be modified, or part of the technical features of the equivalent replacement, within the spirit and principles of the present application, any modification, equivalent replacement, improvement, etc., should be included in the scope of the present application.

Claims

1. A nucleic acid extraction and purification apparatus comprising a purification machine (1), characterized in that: The top of the purification machine (1) is electrically connected with a display screen (2), the bottom of the purification machine (1) is fixedly connected with a plurality of supporting pads (3), one side of the purification machine (1) is rotatably connected with a rotating shaft (4), the inside of the purification machine (1) is provided with a plurality of liquid guide assemblies, the outer wall of the rotating shaft (4) is rotatably connected with a box door (5), the inside of the box door (5) is provided with an observation window (6), one side of the box door (5) is fixedly connected with a handle (7), one side of the purification machine (1) is fixedly connected with a plurality of fixed columns (8), and each fixed column (8) is internally provided with a fixed assembly. Each fixed assembly comprises a fixed pin (9), a limiting plate (10) and a spring (11), the outer wall of each fixed pin (9) is slidably connected in the fixed column (8), the inner wall of each limiting plate (10) is fixedly connected to the outer wall of the fixed pin (9), each spring (11) is arranged in the fixed column (8), one end of each spring (11) is fixedly connected to the inside of the fixed column (8), and the other end of each spring (11) is fixedly connected to the top of the fixed pin (9).

2. The nucleic acid extraction and purification device according to claim 1, wherein: Each liquid guide assembly comprises a moving rod (12), a plurality of liquid guide pipes (13), a connecting rod (14) and a plurality of soft rubber tubes (15), one end of each moving rod (12) is slidably connected in the purification machine (1), the top end of each liquid guide pipe (13) is fixedly connected to the bottom of the moving rod (12), the top of each connecting rod (14) is fixedly connected to the bottom end of the liquid guide pipe (13), and the top end of each soft rubber tube (15) is fixedly connected to the bottom of the connecting rod (14).

3. The nucleic acid extraction and purification device of claim 1, wherein: The inside of the purification machine (1) is slidably connected with a pull block (16), and one side of the pull block (16) is fixedly connected with a handle (17).

4. The nucleic acid extraction and purification device of claim 3, wherein: The inside of the pull block (16) is slidably connected with a plurality of sliding blocks (18), and the top of each sliding block (18) is fixedly connected with a moving block (19).

5. The nucleic acid extraction and purification device of claim 4, wherein: One side of each moving block (19) is fixedly connected with a plurality of connecting columns (20), and the bottom of each moving block (19) is slidably connected to the top of the pull block (16).

6. The nucleic acid extraction and purification device of claim 4, wherein: One side of each sliding block (18) is provided with a spring (21), one end of each spring (21) is fixedly connected to one side of the sliding block (18), and the other end of each spring (21) is fixedly connected to the inside of the pull block (16).

7. The nucleic acid extraction and purification device of claim 3, wherein: The top of the pull block (16) is slidably connected with a test tube box (22), and the inside of the test tube box (22) is slidably connected with a plurality of reagent tubes (23).

8. The nucleic acid extraction and purification device of claim 5, wherein: The connecting columns (20) are arranged in parallel array on one side of the moving block (19), and the outer wall of each connecting column (20) is slidably connected in the inside of the test tube box (22).

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