Thermal insulation box for cell strains and culture reagents thereof

Abstract

The utility model provides a cell strain and cell strain culture reagent incubator which comprises an incubator body and an incubator cover, the incubator body is provided with an inner cavity, and the incubator cover is used for sealing a cavity opening of the incubator body; the thermal insulation plate is arranged in the box body so as to divide an inner cavity of the box body into a first chamber and a second chamber; wherein a first cooling working medium is arranged in the first cavity, and a second cooling working medium is arranged in the second cavity; the cell strain storage rack is used for placing cell strain storage tubes and is arranged in the first chamber; the reagent storage rack is used for placing a reagent storage tube and is arranged in the second chamber. In conclusion, the inner cavity of the incubator is divided into the two independent temperature zones, and the temperature zones are mutually independent and do not influence each other, so that the storage and transportation of the cell strains and the culture reagents thereof are realized at the same time, and the related cost is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of microbiology device, concretely relates to a cell strain and its culture reagent incubator. BACKGROUND

[0002] Cell strain resource sharing is an indispensable link in biological research, and experimental cell strains are also widely used in important fields such as biopharmaceuticals, gene therapy and biological research. When providing cell strain technical services to the outside world, many scientific research users often choose to transport cell strain cryopreservation tubes or live cells according to their own experimental needs and cell strain application directions. Among them, dry ice transportation is the most common way to provide cell strain cryopreservation tubes, which can be transported for a short distance or a medium-short period by placing dry ice in a plastic foam box (-78 DEG C or lower temperature) and then placing the cell strain cryopreservation tube in the dry ice.

[0003] When recovering the cryopreservation tube cell strain, the complete culture medium matched with the cell strain needs to be used synchronously to ensure the survival rate and growth state of the cell strain, but the culture reagents related to the cell strain (such as complete culture medium, trypsin digestion solution, serum, etc.) only need to be stored and transported at low temperature (-0-10 DEG C).

[0004] It can be seen that, since the storage temperature of the cell strain cryopreservation tube and the storage temperature of the matched culture medium do not match, the two cannot be stored and transported in the same device at the same time, and separate storage and transportation of each other will increase the transportation cost, so how to transport the culture reagents matched with the cell strain at the same time when transporting the cell strain cryopreservation tube by dry ice is a problem that needs to be solved urgently. UTILITY MODEL CONTENT

[0005] The utility model is just to solve the above-mentioned technical problem, and its purpose is to provide a cell strain and its culture reagent incubator, which can transport the cell strain cryopreservation tube and the culture reagents matched with the cell strain in the same device at the same time.

[0006] The utility model discloses a cell strain and its culture reagent incubator, which comprises a box body and a box cover, the box body has an inner cavity, and the box cover is used for sealing the cavity opening of the box body;A temperature insulation plate is arranged in the box body to divide the inner cavity of the box body into a first chamber and a second chamber;Among them, the first cooling working medium is arranged in the first chamber, and the second cooling working medium is arranged in the second chamber;A cell strain storage rack for placing cell strain storage tubes is arranged in the first chamber;A reagent storage rack for placing reagent storage tubes is arranged in the second chamber.

[0007] Optionally, one of the box body and the box cover is provided with a sealing groove, and the other is provided with a sealing strip, the sealing strip and the sealing groove are embedded with each other to surround the cavity opening of the box body.

[0008] Optionally, the temperature recorder is arranged on the box cover, and the probe of the temperature recorder is arranged in the clamping groove.

[0009] Optionally, the cell strain storage rack is provided with a hollow structure, and the first cooling medium can flow through the hollow structure to contact the cell strain storage tube.

[0010] Optionally, the cell strain storage rack comprises a mounting plate and a bottom plate arranged at intervals, and a supporting rod connected with the mounting plate and the bottom plate respectively, and the mounting plate and the bottom plate are hollow structures; the mounting plate is provided with a through hole, and the bottom plate is provided with a positioning hole; the cell strain storage tube is inserted into the through hole of the mounting plate, and the bottom of the cell strain storage tube is accommodated into the positioning hole of the bottom plate.

[0011] Optionally, the edge of the cell strain storage rack is provided with a notch, and the notch and the inner wall of the box body form a first cooling flow channel for the flow of the first cooling medium, and the first cooling flow channel is communicated with the hollow structure.

[0012] Optionally, the reagent storage rack comprises two pairs of side plates arranged at intervals, and a reagent storage body located between the two side plates; the edge portion of the side plate is staggered with the reagent storage body, so that the two side plates, the reagent storage body and the inner wall of the box body form a second cooling flow channel for the flow of the second cooling medium.

[0013] Optionally, the reagent storage body and the peripheral wall of the box body, and the bottom wall of the box body are arranged at intervals.

[0014] Optionally, the cell strain storage tube comprises a general tube and a cell cryopreservation tube, and the cell cryopreservation tube is arranged in the general tube.

[0015] Optionally, the box body comprises a waterproof layer, an intermediate thermal insulation layer and a thermal insulation shell arranged in sequence from inside to outside.

[0016] The beneficial effects of the utility model are as follows:

[0017] The utility model discloses a cell strain and culture reagent incubator thereof, include: box body and box cover, and the box body has the inner chamber, and the box cover is used for covering the chamber mouth of box body, the temperature recorder is arranged on the box cover, and the probe of the temperature recorder is arranged in the clamping groove.

[0018] The arrangement of the cell strain storage rack and the reagent storage rack has the buffering effect of pressure resistance and shock absorption, can fully fix the cell strain storage tube and the reagent storage tube, more effectively prevent the damage of cell strain in the cell strain storage tube and reagent in the reagent storage tube due to bumping and dry ice extrusion, and prevent biological sample cross contamination.

[0019] In conclusion, the incubator realizes the storage and transportation of cell strains and culture reagents at the same time by separating the inner cavity into two independent temperature zones, and the temperature zones are independent of each other and do not affect each other, thereby reducing the relevant cost. BRIEF DESCRIPTION OF DRAWINGS

[0020] The above features and advantages of the present application can be better understood by reading the detailed description of embodiments of the present application in conjunction with the following drawings, in which: the components in the drawings are not necessarily drawn to scale, and components having similar related properties or features can have the same or similar reference numerals.

[0021] Figure 1 is the external view of the incubator of the present application;

[0022] Figure 2 is the internal structure diagram of the incubator of the present application;

[0023] Figure 3 is the top view of the incubator of the present application; Figure 2

[0024] Figure 4 is the structure diagram of the incubator of the present application without the box body and the temperature insulation plate; Figure 2

[0025] Figure 5 is the structure diagram of the cell strain storage tube of the present application.

[0026] BRIEF DESCRIPTION OF DRAWINGS

[0027] 100 - box body,

[0028] 101 - first chamber, 102 - second chamber,

[0029] 103 - first cooling flow channel, 104 - second cooling flow channel,

[0030] 110 - sealing groove,

[0031] 200 - box cover,

[0032] 300 - temperature insulation plate,

[0033] 400 - cell strain storage tube,

[0034] 410 - general tube, 411 - tube body, 412 - tube cover,

[0035] 420 - cell cryopreservation tube,

[0036] 500 - cell strain storage rack,

[0037] 510 - clamping groove,

[0038] 520 - hollow structure, ​​

[0039] 530 - Mounting plate, 540 - Base plate, 550 - Support rod

[0040] 560-gap,

[0041] 600-Reagent Storage Tube

[0042] 700-Reagent Storage Rack

[0043] 710 - Side plate, 720 - Reagent storage container,

[0044] 800-Temperature recorder. Detailed Implementation

[0045] The present invention will be further described below with reference to specific embodiments and accompanying drawings. More details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention can obviously be implemented in many other ways different from those described herein. Those skilled in the art can make similar extensions and derivations based on actual application situations without departing from the spirit of the present invention. Therefore, the scope of protection of the present invention should not be limited by the content of this specific embodiment.

[0046] It should be noted that these and other accompanying drawings are merely examples and are not drawn to scale, and should not be used as a limitation on the scope of protection of this utility model.

[0047] Because the storage temperature of cell line cryopreservation tubes and their accompanying culture media are incompatible, they cannot be stored and transported simultaneously in the same device. Separate storage and transport would increase transportation costs. Therefore, to solve this problem, the technical solution of this application was developed. The following is a detailed explanation... Figures 1-5 To elaborate.

[0048] Figure 1 This is an external view of the insulated box of this utility model; Figure 2 This is a diagram of the internal structure of the insulated box of this utility model; Figure 3 This is a utility model Figure 2 A top view of the cell line and its culture reagents. As shown in the figure, this application discloses a cell line and its culture reagents incubator. The incubator includes a body 100, a lid 200, an insulation board 300, a cell line storage rack 500, and a reagent storage rack 700.

[0049] The box body 100 has an inner cavity for accommodating cell strains and culture reagents; the box body 100 comprises a waterproof layer, an intermediate thermal insulation layer and a thermal insulation shell arranged in sequence from inside to outside, the waterproof layer can adopt an aluminum foil film, the intermediate thermal insulation layer can adopt a thermal insulation material mainly of polyurethane, and the thermal insulation shell is mainly of ABS resin material; the overall thickness of the box body 100 is about 3-8 cm, which has the advantages of good thermal insulation performance, waterproofness, lightness, high strength, shock resistance and drop resistance, etc. The box cover 200 is used to cover the cavity opening of the box body 100 to ensure the sealing of the cell strains and culture reagents stored in the inner cavity of the box body 100.

[0050] The thermal insulation plate 300 is arranged in the box body 100 to divide the inner cavity of the box body 100 into a first chamber 101 and a second chamber 102; wherein dry ice or the like is arranged in the first chamber 101 as a first cooling working medium, and a small amount of ice bag or foam or the like is arranged in the second chamber 102 as a second cooling working medium, so that the temperature zones of the first chamber 101 and the second chamber 102 are different, specifically, the first chamber 101 is in a -78℃ temperature zone, and the second chamber 102 is in a -0-10℃ temperature zone. The thermal insulation plate 300 is mainly composed of ultra-high molecular weight polyethylene (UHMWPE), which can divide the incubator into multiple sealed areas, and has the advantages of low temperature resistance of -80℃, high low-temperature impact strength, waterproofness, and anti-permeability, etc., fully ensuring the independence and stability of the temperature zones in the chambers in the box body 100.

[0051] The cell strain storage rack 500 and the reagent storage rack 700 are both of pearl wool material, wherein the cell strain storage rack 500 is arranged in the first chamber 101, and the cell strain storage rack 500 is provided with a plurality of preset holes for placing cell strain storage tubes 400. The cell strain storage tubes 400 store cell strains, and the capacity of the cell strain storage tubes 400 is set to 1-50 mL according to needs, and the shape thereof can be any desired shape such as circular, oval, square, etc. A plurality of cell strain storage tubes 400 can be stored in the cell strain storage rack 500, for example, six cell strain storage tubes 400 can be stored in the cell strain storage rack 500 in two rows and three columns.

[0052] The reagent storage rack 700 is arranged in the second chamber 102, and the reagent storage rack 700 is provided with a plurality of preset holes for placing reagent storage tubes 600. The reagent storage tubes 600 store reagents matched with the cell strains in the cell strain storage tubes 400, such as complete culture medium, trypsin digestion solution, serum, etc.; the capacity of the reagent storage tubes 600 is set to 15-500 mL according to needs, and the shape thereof can be any desired shape such as circular, oval, square, etc. A plurality of reagent storage tubes 600 can be stored in the reagent storage rack 700, for example, six reagent storage tubes 600 can be stored in the reagent storage rack 700 in two rows and three columns.

[0053] The cell strain storage rack 500 and the reagent storage rack 700 are provided with compression resistance and damping buffering effects, so that the cell strain storage tube 400 and the reagent storage tube 600 can be fixed sufficiently, and damage to the cell strain in the cell strain storage tube 400 and the reagent in the reagent storage tube 600 caused by jolting and dry ice extrusion can be prevented more effectively, and cross contamination of biological samples can be prevented.

[0054] In summary, the incubator can realize storage and transportation of cell strains and culture reagents at the same time by dividing the inner cavity into two independent temperature zones which are independent of each other, thereby reducing the relevant cost.

[0055] Optionally, one of the box body 100 and the box cover 200 is provided with the sealing groove 110, and the other is provided with the sealing strip, the sealing strip and the sealing groove 110 are matched with each other to surround the cavity opening of the box body 100; for example, the box body 100 is provided with the sealing strip, and the box cover 200 is provided with the sealing groove 110; or as shown in the box body 100 is provided with the sealing groove 110, and the box cover 200 is provided with the sealing strip, the sealing groove 110 is an annular groove and surrounds the cavity opening of the box body 100, and the sealing strip is matched with the sealing groove 110 in shape. Figure 2 The sealing strip and the sealing groove 110 matched with each other can improve the sealing performance of the incubator, prevent the loss of internal cold energy, and prevent external impurities from polluting.

[0056] Optionally, the box cover 200 is provided with the temperature recorder 800, and at least one of the cell strain storage rack 500 and the reagent storage rack 700 is provided with the clamping groove 510; for example, only the cell strain storage rack 500 is provided with the clamping groove 510, or only the reagent storage rack 700 is provided with the clamping groove 510, or both the cell strain storage rack 500 and the reagent storage rack 700 are provided with the clamping groove 510. The probe of the temperature recorder 800 is arranged in the clamping groove 510, the high-precision probe of the ultra-low temperature temperature recorder containing a temperature sensing chip can accurately measure a temperature change interval of-99.9℃-99℃. In this way, the temperature recorder 800 can be observed, the real-time temperature in the incubator can be monitored in time and accurately, a PDF format temperature report can be automatically generated through a USB interface connected with a computer, the whole process of temperature change of each temperature zone in the incubator can be traced, and the requirement of air transportation can be met.

[0057] Figure 4 The incubator Figure 2 removes the structure of the box body and the temperature insulation plate, the specific structure of the cell strain storage rack 500 and the reagent storage tube 600 is further shown in the figure, and the cell strain storage rack 500 is further introduced as follows:

[0058] As shown in the drawings, the cell strain storage rack 500 is provided with a hollow structure 520; through the arrangement of the hollow structure 520, on the one hand, the dry ice as the first cooling working medium can flow through the hollow structure 520 until fully contacting the cell strain storage tube 400, improving the cooling preservation effect of the cell strain storage tube 400; on the other hand, the convection of cold air can be realized, ensuring the stability of the temperature zone.

[0059] Further, the cell strain storage rack 500 comprises a mounting plate 530 and a bottom plate 540 arranged at intervals, and a support rod 550 connecting the mounting plate 530 and the bottom plate 540 respectively, and the hollow structure 520 is arranged between the mounting plate 530 and the bottom plate 540; the mounting plate 530 is provided with a through hole, and the bottom plate 540 is provided with a positioning hole, the cell strain storage tube 400 is inserted into the through hole of the mounting plate 530, and the bottom of the cell strain storage tube 400 is accommodated into the positioning hole of the bottom plate 540. In this way, on the one hand, the hollow structure 520 formed is larger, further ensuring the full contact of the dry ice and the cell strain storage tube 400; on the other hand, the cell strain storage tube 400 can be fixed well, preventing displacement during transportation.

[0060] Further, the bottom plate 540 is spaced apart from the bottom wall of the box body 100, so as to better realize the flow of the dry ice in the first chamber 101.

[0061] Further, in combination with Figures 2-4 As shown in the drawings, the mounting plate 530 edge and the bottom plate 540 edge of the cell strain storage rack 500 are provided with notches 560, and the notches 560 and the inner wall of the box body 100 form a first cooling flow channel 103 for the flow of the dry ice as the first cooling working medium, and the first cooling flow channel 103 is communicated with the hollow structure 520, so as to improve the flow performance of the dry ice in the first chamber 101, and better realize the cooling preservation of the cell strain storage tube 400.

[0062] Further, the front wall and the rear wall of the box body 100 are arranged opposite along the length direction of the heat preservation box, the heat insulation plate 300 is located between the front wall and the rear wall, and gaps are reserved between the cell strain storage rack 500 and the front wall of the box body 100 and between the cell strain storage rack 500 and the heat insulation plate 300, so that the dry ice and other first cooling working medium can have a larger flow space in the first chamber 101, further improving the flow performance of the dry ice, improving the convection effect of the cold air, and improving the cooling preservation effect of the cell strain storage tube 400.

[0063] The following will be further introduced Figures 2-4 The reagent storage rack 700 is further introduced:

[0064] The reagent storage rack 700 comprises two pairs of side plates 710 and a reagent storage body 720 between the two side plates 710, and the reagent storage body 720 is used for storing the reagent storage tube 600. The edge part of the side plate 710 is staggered with the reagent storage body 720, so that the second cooling flow channel 104 for the second cooling medium such as ice bag is formed between the two side plates 710, the reagent storage body 720 and the inner wall of the box body 100, so as to ensure the flow and convection effect of the cold air in the second cavity 102, and then ensure the cooling of the reagent storage tube 600.

[0065] Further, due to the support of the side plate 710, the reagent storage body 720 and the peripheral wall of the box body 100, and the bottom wall of the box body 100 are spaced apart, so that the second cooling flow channel 104 surrounds the reagent storage body 720, and the cold air can flow around the peripheral surface and the bottom surface of the reagent storage body 720 along the second cooling flow channel 104, thereby improving the cooling effect of the reagent storage tube 600 located therein.

[0066] Further, one side plate 710 is attached to the temperature insulation plate 300, and a gap is reserved between the other side plate 710 and the rear wall of the box body 100 to ensure the storage space of the second cooling medium such as ice bag, thereby ensuring the cooling effect of the reagent storage tube 600.

[0067] Figure 5 The utility model cell strain storage tube's structure diagram is as shown in the drawing, cell strain storage tube 400 includes general pipe 410 and cell cryopreservation tube 420, cell cryopreservation tube 420 is located in general pipe 410. Specifically, general pipe 410 includes pipe body 411 and pipe cover 412, and pipe body 411 and pipe cover 412 are all sterile polypropylene material, cell cryopreservation tube 420 is stored in pipe body 411, and pipe cover 412 covers the pipe mouth of pipe body 411 to realize the sealing of cell cryopreservation tube 420.

[0068] The above setting is considered that the friction of dry ice in the logistics transportation process may have the risk of breaking the cell cryopreservation tube 420, so the cell cryopreservation tube 420 is placed in the general pipe 410, and then the general pipe 410 is embedded in the reagent storage rack 700 with the same size, so that the violent behavior of dry ice transportation can be effectively avoided.

[0069] The specific implementation of the cell strain and its culture reagent storage using the heat preservation box of the present application is introduced as follows:

[0070] First, the cell cryopreservation tube 420 to be transported is taken out from the liquid nitrogen tank by using special tools, and is immediately placed in the pipe body 411 of the general pipe 410 with the same specification and slightly larger than the cell cryopreservation tube 420, and then the pipe cover 412 is tightened, to constitute the cell strain storage tube 400 and be temporarily stored in the-80 DEG C refrigerator.

[0071] A small amount of dry ice is poured into the first chamber 101 as the first cooling medium along the inner wall of the box 100 until the bottom of the box is covered, at which time the first chamber 101 constitutes a -78℃ temperature zone.

[0072] The cell strain storage tubes 400 are respectively placed in the corresponding preset holes of the cell strain storage rack 500, and the reagent storage tubes 600 are respectively placed in the corresponding preset holes of the reagent storage rack 700, so as to fix the cell strain storage tubes 400 and the reagent storage tubes 600 and prevent them from shaking due to the jolt of logistics and the extrusion of dry ice.

[0073] The temperature sensing probe of the temperature recorder 800 is placed in the clamping groove 510 area of the cell strain storage rack 500 and the reagent storage rack 700.

[0074] The first chamber 101 is filled with dry ice as the first cooling medium until the entire cell strain storage rack 500 is completely covered.

[0075] A small amount of ice bag or foam is placed in the second chamber 102 as the second cooling medium.

[0076] The sealing groove 110 and the sealing strip are sealingly fitted, so as to tightly combine the box cover 200 and the box body 100 to complete the sealing of the box.

[0077] The data of the temperature recorder 800 placed in the box cover of the incubator is observed to timely and accurately monitor the real-time temperature change in the incubator. Through data monitoring: due to the isolation effect of the temperature insulation plate 300, even when the first chamber 101 in the -78℃ temperature zone is filled with dry ice and placed for 48-72h, the 0-10℃ temperature zone formed in the second chamber 102 will not have the culture reagent frozen, which completely meets the synchronous transportation demand of the scientific research users for the experimental frozen cell strains and the matching culture reagents.

[0078] The utility model discloses although the above-mentioned preferable embodiment is disclosed, but it is not used to limit the utility model, and any person skilled in the art can make possible change and modification without departing from the spirit and scope of the utility model. Therefore, any modification, equivalent change and modification made to the above-mentioned embodiment according to the technical essence of the utility model, all fall within the protection scope defined by the utility model claim.

Claims

1. A cell line and its culture reagent incubator, characterized in that, The application relates to a cell bank storage box, which comprises a box body (100) and a box cover (200), the box body (100) has an inner cavity, and the box cover (200) is used for covering the cavity opening of the box body (100); a thermal insulation plate (300) is arranged in the box body (100) and is used for dividing the inner cavity of the box body (100) into a first cavity (101) and a second cavity (102); the first cavity (101) is provided with a first cooling working medium, and the second cavity (102) is provided with a second cooling working medium; a cell bank storage rack (500) for placing cell bank storage tubes (400) is arranged in the first cavity (101); and a reagent storage rack (700) for placing reagent storage tubes (600) is arranged in the second cavity (102). One of the box body (100) and the box cover (200) is provided with a sealing groove (110), and the other is provided with a sealing strip, the sealing strip and the sealing groove (110) are embedded with each other to surround the cavity opening of the box body (100). The box cover (200) is provided with a temperature recorder (800), At least one of the cell bank storage rack (500) and the reagent storage rack (700) is provided with a clamping groove (510), and a probe of the temperature recorder (800) is arranged in the clamping groove (510). The cell bank storage rack (500) is provided with a hollow structure (520), and the first cooling working medium can flow to the cell bank storage tubes (400) through the hollow structure (520).

2. The incubator of claim 1, wherein, The cell bank storage rack (500) comprises a mounting plate (530) and a bottom plate (540) which are arranged at intervals, and a supporting rod (550) connected with the mounting plate (530) and the bottom plate (540) respectively, and the hollow structure (520) is arranged between the mounting plate (530) and the bottom plate (540).

3. The incubator of claim 1, wherein, The mounting plate (530) is provided with a through hole, the bottom plate (540) is provided with a positioning hole, the cell bank storage tube (400) is inserted into the through hole of the mounting plate (530), and the bottom of the cell bank storage tube (400) is accommodated into the positioning hole of the bottom plate (540). The edge of the cell bank storage rack (500) is provided with a notch (560), and the notch (560) and the inner wall of the box body (100) surround a first cooling flow channel (103) for the flow of the first cooling working medium, and the first cooling flow channel (103) communicates with the hollow structure (520).

4. The incubator of claim 1, wherein, The reagent storage rack (700) comprises two pairs of side plates (710) and a reagent storage body (720) arranged between the two pairs of side plates (710), 5. An incubator according to claim 4, wherein The edge portion of the side plate (710) is staggered with the reagent storage body (720), so that the second cooling flow channel (104) for the flow of the second cooling working medium is surrounded by the two side plates (710), the reagent storage body (720) and the inner wall of the box body (100). The reagent storage body (720), the peripheral wall of the box body (100) and the bottom wall of the box body (100) are arranged at intervals.

6. The incubator of claim 4, wherein, ​ 7. The incubator of claim 1, wherein ​ ​ 8. The incubator of claim 7, wherein, ​ 9. The incubator of claim 1, wherein, The cell bank storage tube (400) comprises a general tube (410) and a cell cryopreservation tube (420), and the cell cryopreservation tube (420) is arranged in the general tube (410).

10. The incubator of claim 1, wherein, The box (100) comprises a waterproof layer, an intermediate thermal insulation layer and a thermal insulation shell arranged in sequence from inside to outside.

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