Sample loading device of bioreactor

Abstract

The utility model discloses a bioreactor's sample loading device, including reactor shell and connecting the discharge pipe on the reactor shell, the top of reactor shell is sealed with the top cover, the top cover is provided with the stirring motor, the output end of the stirring motor is fixedly connected with the stirring shaft, the stirring shaft is provided with the stirring blade, and the stirring blade is provided with the discharge pipe. A sample adding opening is further formed in the top cover, a sample adding pipe is connected to the sample adding opening, and a sample injection pipe is fixedly connected to the lower portion of the sample adding opening; an annular material receiving plate is fixed to the top end of the stirring shaft, a sample feeding pipe is fixed to one side of the annular material receiving plate, an inclined opening is formed in the joint of the sample feeding pipe and the annular material receiving plate, the other end of the sample feeding pipe naturally extends downwards in the reactor shell, and the bottom end of the sample feeding pipe is aligned to the annular material receiving plate. The utility model belongs to the technical field of bioreactors, and particularly relates to a sampling device of a bioreactor.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of bioreactor, specifically point to a kind of sampling device of bioreactor. BACKGROUND

[0002] In the field of biological reaction engineering, bioreactor is widely used in microbial fermentation, cell culture and other biological processes. At present, the sample adding position of many bioreactors is fixed, and the newly added sample may be concentrated in a local area, which is difficult to mix with the original reaction system in the reactor quickly, resulting in uneven distribution of the sample in the reactor, affecting the utilization efficiency of microorganisms to substrate, and further reducing the fermentation efficiency and product quality. CONTENT

[0003] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a kind of sampling device of bioreactor, effectively solve the problem of fixed sample adding position, affect the utilization efficiency of microorganisms to substrate.

[0004] In order to realize the above function, the technical scheme adopted by the utility model is as follows: a kind of sampling device of bioreactor, including reactor shell and the discharge pipe connected to the reactor shell, the top of the reactor shell is sealed with top cover, the top cover is equipped with stirring motor, the output end of the stirring motor is fixedly connected with stirring shaft, the stirring shaft is equipped with stirring blade, the top cover is also equipped with sample adding port, the sample adding port is connected with sample adding pipe, the lower part of the sample adding port is fixedly connected with sample inlet pipe;

[0005] The top end of the stirring shaft is fixed with annular material receiving plate, one side of the annular material receiving plate is fixed with sample feeding pipe, the connection between the sample feeding pipe and the annular material receiving plate is provided with bevel, and the other end of the sample feeding pipe extends naturally downward in the reactor shell, and the bottom end of the sample inlet pipe is aligned with the annular material receiving plate.

[0006] Preferably, the bottom of the annular material receiving plate is fixedly connected with J-shaped fixing frame, and the top end of the fixing frame is fixed on the top cover.

[0007] Preferably, the bottom of the annular material receiving plate is fixedly connected with J-shaped fixing frame, and the top end of the fixing frame is fixed on the top cover.

[0008] Preferably, the annular material receiving plate is concave in the middle, and the bottom surface of the concave is inclined to one side of the bevel, the bottom of the bevel and the concave surface of the annular material receiving plate are continuous inclined surface, and the cross section is isosceles trapezoid.

[0009] Preferably, the bottom of the reactor shell is provided with air inlet, and the top cover is also provided with air outlet.

[0010] Preferably, the sample adding pipe is an elastic hose, and the other end of the sample adding pipe is externally connected with a peristaltic pump.

[0011] The beneficial effects of the above structure of the utility model are as follows: the annular material receiving plate is driven to rotate by the stirring shaft driven by the stirring motor, the upper sample pipe is driven to rotate by the annular material receiving plate, so that the sample is arranged at positions around the stirring shaft, and the sample at the upper sample pipe also falls on the material receiving guide plate in the rotating process, falls on different positions in different directions in the reaction shell along the material receiving guide plate, so that the sample is uniformly distributed in the reactor, and the utilization efficiency of the microorganism on the substrate is improved. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 The utility model provides a kind of overall structure schematic diagram of the sample feeding device of bioreactor;

[0013] Figure 2 The utility model provides a kind of section of the sample feeding device of bioreactor Figure 1 ;

[0014] Figure 3 The utility model provides a kind of section of the sample feeding device of bioreactor Figure 2 ;

[0015] Figure 4 For Figure 3 Local enlarged view in A of the utility model;

[0016] Figure 5 The utility model provides a kind of structure schematic diagram of the material receiving guide plate of the sample feeding device of bioreactor.

[0017] Wherein, 1, reactor shell, 2, top cover, 3, stirring motor, 4, stirring shaft, 5, stirring blade, 6, sample adding port, 7, sample adding pipe, 8, sample inlet pipe, 9, annular material receiving plate, 10, upper sample pipe, 11, bevel, 12, fixed frame, 13, fixed ring plate, 14, material receiving guide plate, 15, discharge pipe, 16, air inlet, 17, exhaust port. DETAILED DESCRIPTION

[0018] The technical scheme of the utility model will be described clearly and completely in connection with the drawings, obviously, the described embodiment is a part of the embodiment of the utility model, not all the embodiment. Based on the embodiment in the utility model, all other embodiments obtained by the person skilled in the art without making creative labor belong to the scope of protection of the utility model.

[0019] In the description of the utility model, it is necessary to explain that the orientation or positional relation indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like is the orientation or positional relation based on the orientation or positional relation shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as limiting the utility model. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance. The utility model will be further described in detail below in combination with the drawings.

[0020] As shown in Figures 1-5 The utility model provides a kind of feeding device of bioreactor, including reactor shell 1 and the discharge pipe 15 connected to reactor shell 1, reactor shell 1 top is sealed with top cover 2, reactor shell 1 bottom is equipped with discharge pipe 15 and air inlet 16, top cover 2 is also equipped with exhaust port 17, top cover 2 is equipped with stirring motor 3, stirring motor 3 output end is fixedly connected with stirring shaft 4, stirring shaft 4 is equipped with stirring blade 5, top cover 2 is also equipped with sample addition port 6, sample addition port 6 is connected with sample addition pipe 7, sample addition port 6 lower part is fixedly connected with sample inlet tube 8;Stirring shaft 4 top end is fixed with annular material receiving plate 9, annular material receiving plate 9 one side is fixed with feeding tube 10, the junction of feeding tube 10 and annular material receiving plate 9 is equipped with bevel 11, and other end of feeding tube 10 is extended downwards in reactor shell 1 inside naturally, sample in annular material receiving plate 9 is guided into feeding tube 10, falls from feeding tube 10, sample inlet tube 8 bottom end is aligned with annular material receiving plate 9, and sample is added to annular material receiving plate 9, sample addition pipe 7 is elastic hose, other end of sample addition pipe 7 is connected with peristaltic pump, under the condition of guaranteeing sample activity and not being contaminated, biological sample is added to bioreactor.

[0021] As shown in Figure 5 Annular material receiving plate 9 is equipped with fixed ring plate 13 below, fixed ring plate 13 is sleeved in stirring shaft 4, and multiple long and short material receiving guide plates 14 are evenly arranged around fixed ring plate 13, material receiving guide plate 14 is inclinedly arranged, and the section is U-shaped, so as to guide sample to different positions in each direction and uniformly distribute sample.

[0022] As shown in Figure 2 , 5 Fixed frame 12 of J type is fixedly connected to the bottom of fixed ring plate 13, and the top end of fixed frame 12 is fixed on top cover 2, so as to fix the position of fixed ring plate 13.

[0023] As shown in Figure 4 , 5As shown, the annular sample receiving plate 9 is concave in the middle, and the bottom of the concave surface is inclined to one side of the beveled opening 11, the bottom of the beveled opening 11 and the concave surface of the annular sample receiving plate 9 form a continuous inclined surface, and the cross section is isosceles trapezoidal, so that the sample in the annular sample receiving plate 9 passes through the beveled opening 11 along the bottom surface into the sample loading tube 10.

[0024] In specific use, the other end of the sample loading tube 7 is connected with a peristaltic pump, the sample is sent into the sample loading tube 7 through the peristaltic pump, and then passes through the sample loading tube 7, the sample loading port 6 and the annular sample receiving plate 9, the stirring motor 3 drives the stirring shaft 4 and the stirring blade 5 to rotate, so that the culture solution, substrate and microorganisms in the reactor are mixed uniformly, the stirring shaft 4 drives the annular sample receiving plate 9 to rotate in the process of rotating, the annular sample receiving plate 9 drives the sample loading tube 10 to rotate, so that the sample is arranged at positions around the stirring shaft 4, and the sample at the sample loading tube 10 also falls on the sample receiving guide plate 14 in the process of rotating, and then falls on different positions in different directions in the reaction shell along the sample receiving guide plate 14, so that the sample is uniformly distributed in the reactor, and the utilization efficiency of the substrate by the microorganisms is improved.

[0025] The above describes the present application and its embodiments, which are not limited, and the embodiments shown in the drawings are only one of the embodiments of the present application, and the actual structure is not limited thereto. In summary, if a person skilled in the art is inspired by the above description, without departing from the spirit of the present application, similar structural modes and embodiments can be designed without creative design, which should belong to the protection scope of the present application.

Claims

1. A loading device for a bioreactor, characterized by: Including reactor shell (1) and the discharge pipe (15) connected to reactor shell (1), the top of the reactor shell (1) is sealed with a top cover (2), the top cover (2) is provided with a stirring motor (3), the output end of the stirring motor (3) is fixedly connected with a stirring shaft (4), the stirring shaft (4) is provided with a stirring blade (5), the top cover (2) is further provided with a sample addition port (6), the sample addition port (6) is connected with a sample addition tube (7), the lower part of the sample addition port (6) is fixedly connected with a sample inlet tube (8); The top end of the stirring shaft (4) is fixed with an annular material receiving plate (9), one side of the annular material receiving plate (9) is fixed with a sample feeding tube (10), the connection between the sample feeding tube (10) and the annular material receiving plate (9) is provided with an inclined port (11), and the other end of the sample feeding tube (10) naturally extends downward inside the reactor shell (1), and the bottom end of the sample inlet tube (8) is aligned with the annular material receiving plate (9).

2. The loading device of a bioreactor according to claim 1, characterized in that: The annular material receiving plate (9) is provided below with a fixed ring plate (13), the fixed ring plate (13) is sleeved outside the stirring shaft (4), a plurality of material receiving guide plates (14) with different lengths are uniformly arranged around the fixed ring plate (13), the material receiving guide plates (14) are inclined, and the cross section is U-shaped.

3. The loading device of a bioreactor according to claim 2, characterized in that: The bottom of the fixed ring plate (13) is fixedly connected with a J-shaped fixing frame (12), and the top end of the fixing frame (12) is fixed on the top cover (2).

4. The loading device of a bioreactor according to claim 3, characterized in that: The annular material receiving plate (9) is concave in the middle, and the inner concave bottom surface is inclined to one side of the inclined port (11), the bottom of the inclined port (11) and the inner concave surface of the annular material receiving plate (9) form a continuous inclined surface, and the cross section is isosceles trapezoidal.

5. The loading device of a bioreactor according to claim 4, characterized in that: The bottom of the reactor shell (1) is provided with an air inlet (16), and the top cover (2) is further provided with an air outlet (17).

6. A loading device for a bioreactor according to any one of claims 1-5, characterized in that: The sample addition tube (7) is a flexible hose, and the other end of the sample addition tube (7) is connected with a peristaltic pump.

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