Fluorescence signal detection disc for corn seeds

Abstract

The utility model relates to the technical field of fluorescence signal detection, and discloses a corn seed fluorescence signal detection disc which comprises a polycarbonate microwell plate which is of a cuboid structure, a plurality of sample placing holes distributed in a rectangular array are formed in the surface of the polycarbonate microwell plate in a penetrating mode, the polycarbonate microwell plate is used for bearing corn seeds, and hydrophobic coatings are arranged on the sample placing holes to reduce seed adhesion residues. The microporous plate of the device adopts a standard 96 / 384-hole rectangular array layout, can detect hundreds of corn seeds at a time, is adaptive to an automatic fluorescence sorter, remarkably improves the detection efficiency, adopts a modular stacking structure, supports more than 10 layers of stacked placement, saves 80% of storage space, realizes continuous detection of batch samples, and has the characteristics of strong practicability and high flux.

Description

TECHNICAL FIELD

[0001] The utility model relates to fluorescent signal detection technical field, concretely is a corn seed fluorescent signal detection disc. BACKGROUND

[0002] As a global core food crop, the quality of corn seeds is directly related to yield and stress resistance. Traditional detection relies on germination tests (time-consuming 7-15 days) or molecular detection (such as PCR, 2-3 days), which is long and destroys the sample. Fluorescence detection technology, with its non-destructive, high sensitivity and fast response (completed within a few hours), has become a core means for seed vigor evaluation, pest screening, gene editing and transgenic trait screening. By exciting endogenous fluorescent substances (chlorophyll, phenolic compounds), exogenous fluorescent genes or exogenous marker probes (fluorescent dyes, quantum dots), physiological state, pathogen infection or target gene expression data can be quantitatively obtained, significantly accelerating breeding screening and commercial grading process.

[0003] Early fluorescence detection equipment (such as handheld fluorescence meter, microscope coupled spectrometer) requires placing seeds one by one and manually positioning the excitation light spot. Only 1-5 seeds can be detected at a time. This method has the following significant defects: low efficiency: the operator needs to adjust the seed position repeatedly, and the processing capacity per hour is less than 200 seeds, which is difficult to meet the daily detection needs of breeding enterprises with tens of thousands of seeds; poor data consistency: manual operation leads to light spot positioning deviation (error ≥20%), affecting the comparability of fluorescence signals; sample damage risk: frequent contact may damage the surface structure of the seed, especially for coated seeds or embryo detection, which significantly interferes with the detection.

[0004] To improve the detection throughput, some laboratories use 24-well or 96-well cell culture plates as carriers, combined with enzyme labelers for batch detection. Although this method increases the number of seeds detected at a time to dozens, it still has the following bottlenecks: poor aperture compatibility: the aperture of the standard well plate (6-8mm) does not match the size of the corn seed (8-12mm), resulting in seed tilting or stacking, excitation light penetration path blocked, signal attenuation rate as high as 30%-50%; high cleaning complexity: seed residues easily block the filter membrane at the bottom of the well, which requires disassembly of the well plate for ultrasonic cleaning, consuming more than 40% of the total detection time; unable to monitor dynamically: the design of static well plate limits real-time observation of fluorescence changes during seed germination, making it difficult to capture dynamic physiological indicators.

[0005] The core contradiction in the existing technological system lies in the imbalance between detection throughput, cost control, and data accuracy. Manual and low-throughput equipment cannot meet the needs of industrial-scale testing, while high-end automated equipment, due to its complex mechanical structure and customized hardware and software, drives up costs and significantly increases maintenance barriers. Furthermore, the morphological diversity of corn seeds (size, surface texture, coating thickness) further exacerbates the difficulty of standardized testing. According to statistics from agricultural testing institutions, the overall efficiency (effective detection volume per unit cost) of current mainstream technologies is only 10%-15% of the theoretical requirement, becoming a key bottleneck restricting the implementation of precision agriculture technologies. Overcoming this predicament requires collaborative innovation across three dimensions: carrier design, optical system optimization, and automation integration, to build a new generation of testing platforms that balance high throughput, low cost, and high robustness. Utility Model Content

[0006] The purpose of this invention is to provide a corn seed fluorescence signal detection disk to solve the problems mentioned in the background art.

[0007] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a corn seed fluorescence signal detection disk, comprising:

[0008] The polycarbonate microporous plate has a cuboid structure with several sample placement holes arranged in a rectangular array on its surface to hold corn seeds. The sample placement holes are coated with a hydrophobic coating to reduce seed adhesion and residue.

[0009] The upper edge plate is fixed to the upper edge of the polycarbonate microporous plate. It has a notch at the front end for discharging excess seeds, and the upper edge plate has symmetrical slots for picking up and putting down handles on both sides.

[0010] Four corner support blocks are fixed to the lower four corners of the polycarbonate microporous plate. The lower end of the four corner support blocks is provided with right-angled grooves for nesting and overlapping with the upper edge plate of another detection disk at the four corners.

[0011] A polypropylene base plate is detachably installed between the four corner support blocks to support the corn seeds in the polycarbonate microporous plate. It is inserted through a guide groove and a sliding fit with the outer edge plate, and is fixed by a quick-release clip.

[0012] According to the above technical solution, the guide groove of the four corner support blocks has a symmetrical structure, and the four corners of the outer edge plate of the polypropylene base plate are chamfered, so that the polypropylene base plate can be inserted or removed from the gap between any two adjacent four corner support blocks, realizing multi-directional assembly and disassembly.

[0013] According to the above technical solution, the quick card connector includes:

[0014] A telescopic spring is provided inside the polypropylene base plate, with its top end fixed in a placement groove;

[0015] A lifting movable plate connected with the bottom end of the telescopic spring, the lifting movable plate is constrained in vertical movement by a limiting groove;

[0016] An arc-shaped clamping joint fixed to the lower end of the lifting movable plate, the end of the arc-shaped clamping joint is matched and locked with an arc-shaped clamping groove on the outer edge plate.

[0017] According to the technical scheme, the inner wall of the guide sliding groove is sprayed with a polytetrafluoroethylene wear-resistant coating, and the thickness of the coating is 0.5-1.2 mm.

[0018] According to the technical scheme, the lower end surface of the polypropylene bottom plate is 0.5-2 mm higher than the lower end surface of the four corner supporting blocks, and the bottom surface of the polypropylene bottom plate is provided with cross-symmetrically distributed dismounting handle grooves one and two for assisting the taking and placing operation.

[0019] Compared with the prior art, the utility model has the beneficial effects that:

[0020] (1) High flux design: the microwell plate adopts a standard 96 / 384 hole rectangular array layout, and hundreds of corn seeds can be detected at a time, which is suitable for an automatic fluorescence sorting instrument, significantly improves the detection efficiency, saves 80% of storage space, and realizes batch sample continuous detection;

[0021] (2) Quick dismounting mechanism: the polypropylene bottom plate is installed in a "plug-in" mode through the guide sliding groove and the clamping piece, no tools are needed, and the dismounting is completed within 3 seconds by single-handed operation, the detection efficiency is improved, the spring-driven arc-shaped clamping joint of the quick clamping piece is automatically locked, 10,000 cycles of use are supported, the reliability is high, the operation process is simplified, and the high-frequency use scene is suitable;

[0022] (3) Optimal optical performance: the natural polycarbonate is transparent or translucent material, the light transmittance can reach 90%, the ultraviolet light aging resistance is good, the fluorescence excitation light is not attenuated, the signal acquisition error is less than or equal to 2%, the sample hole inner wall is provided with a hydrophobic coating, seed adhesion and residue are reduced, and false positive / negative results caused by sample pollution are avoided;

[0023] (4) Convenient maintenance: the detachable bottom plate supports immersion cleaning, the dead angle structure reduces the cleaning difficulty, the maintenance time is reduced by 50%, the polycarbonate (impact strength ≥ 60 kJ / m²) is combined with the polypropylene (temperature resistance 120 DEG C), and the laboratory common chemical reagents (alcohol, sodium hypochlorite) and high-pressure sterilization (121 DEG C / 30 min) are resistant;

[0024] (5) Multi-directional plug-in: the symmetrical structure of the guide sliding groove and the chamfer design of the outer edge plate allow the bottom plate to be inserted / taken out from the gap between any two adjacent supporting blocks, precise alignment is not needed, and operation mistakes are reduced.

[0025] (6) Flexible compatibility: adapt to standard microwell plate detector, support multi-mode detection such as fluorescence and absorbance, through changing aperture adapter (6-12mm), expand to soybean, wheat and other crop seed detection. BRIEF DESCRIPTION OF DRAWINGS

[0026] The accompanying drawings are included to provide a further understanding of the present application, and constitute a part of the specification, illustrate the present application and explain the technical solutions of the present application, and do not constitute a limitation on the present application. In the drawings:

[0027] Figure 1 is the first three-dimensional schematic view of the present application;

[0028] Figure 2 is the second three-dimensional schematic view of the present application;

[0029] Figure 3 is the third three-dimensional schematic view of the present application;

[0030] Figure 4 is the exploded schematic view of the present application;

[0031] Figure 5 is the first partial three-dimensional schematic view of the present application;

[0032] Figure 6 is the second partial three-dimensional schematic view of the present application;

[0033] Figure 7 is the enlarged schematic view of A in the present application; Figure 5

[0034] Figure 8 is the enlarged schematic view of B in the present application; Figure 6

[0035] In the figure: 1-polycarbonate microwell plate, 101-sample placing hole, 2-upper edge plate, 201-notched area, 202-taking and placing handle groove, 3-four corner supporting block, 301-right angle groove, 302-guiding sliding groove, 4-polycarbonate bottom plate, 401-outer edge plate, 402-placing groove, 403-limiting groove, 404-arc-shaped clamping groove, 405-dismantling handle groove one, 406-dismantling handle groove two, 5-quick clamping piece, 501-elastic spring, 502-lifting movable plate, 503-arc-shaped clamping joint, 504-arc-shaped clamping groove. DETAILED DESCRIPTION

[0036] ​​Clearly and completely describe the technical scheme in the embodiments of the utility model with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all. Based on the embodiments in the utility model, all other embodiments obtained by ordinary skilled in the art without creative labor belong to the scope of the utility model.

[0037] Please refer to Figures 1-8 The utility model provides technical scheme: a corn seed fluorescence signal detection disc, include: polycarbonate microporous plate 1, present cuboid structure, surface is equipped with a plurality of sample placement hole 101 of rectangular array distribution and is set up in the through, for bearing corn seed, sample placement hole 101 sets up hydrophobic coating, reduces seed adhesion residue;

[0038] Upper edge board 2 is fixed in the upper end edge of polycarbonate microporous plate 1, and the front end is provided with a notch area 201 for discharging excess seeds, and the both sides of the upper edge board 2 are symmetrically provided with a taking and placing handle groove 202.

[0039] Four corner support blocks 3 are fixed to the lower end corners of the polycarbonate microporous plate 1, and a right-angle groove 301 is formed in the lower end of the four corner support blocks 3 for nesting and stacking with the upper edge board 2 of another detection disc.

[0040] A polypropylene bottom plate 4 is detachably installed between the four corner support blocks 3 for supporting the corn seeds of the polycarbonate microporous plate 1, and is inserted and connected through the sliding cooperation of the guide sliding groove 302 and the outer edge plate 401, and is limited and fixed through the quick clamping piece 5.

[0041] The corn seed fluorescence detection disc is composed of a polycarbonate microwell plate 1, an upper edge plate 2, a four-corner support block 3, a polypropylene bottom plate 4 and a quick clamping piece 5, forming a modular and stackable detection device suitable for fluorescence screening and batch processing of corn seeds. The polycarbonate microwell plate 1 has high transparency, chemical corrosion resistance and impact resistance, which meets the optical requirements of fluorescence detection. If transmission excitation light is required (such as bottom excitation mode), the microwell plate needs to have high light transmission. If stray light needs to be shielded (such as top excitation mode), it needs to be opaque. In some special instruments, according to the detection target and experimental design, the polycarbonate microwell plate can be made full black and opaque according to the needs. In general application, transparent polycarbonate can meet the needs, taking into account light transmission and cost. High-precision detection requires low-fluorescence and opaque modified materials (full black design) or surface treatment to optimize optical performance. The polycarbonate microwell plate supports optical signal acquisition during fluorescence detection and has a cuboid design. A rectangular array of sample placement holes 101 is provided on the surface. The inner wall of the hole is provided with a hydrophobic coating to reduce seed adhesion. The hole diameter matches the size of the corn seed (about 10-12 mm in diameter) to ensure stable placement of the seed and facilitate the penetration of fluorescence excitation light. The microwell array design improves detection throughput and is compatible with standard microwell plate detectors (such as fluorescence sorting boxes or multifunctional microwell plate detectors). The upper edge plate 2 is adhered to the upper end of the polycarbonate microwell plate 1 by polyurethane glue. The notch area 201 is located at the front end for pouring excess seeds or cleaning residues to prevent seed overflow during operation. The take-and-place handle grooves 202 are symmetrically provided on both sides with a moderate depth to accommodate fingers, improving operational convenience and meeting ergonomic design. The four-corner support block 3 is provided at the lower end of the polycarbonate microwell plate 1. The material is the same as the microwell plate (polycarbonate). The lower end is designed as an L-shaped groove, which is nested with the upper edge plate 2 of another detection disc at the four corners to achieve vertical stacking, saving storage space and realizing modular stacking of the detection disc for batch processing. The polypropylene bottom plate 4 is lightweight, heat-resistant and chemically stable, suitable for frequent disassembly and cleaning to avoid cross-contamination when detecting different batches of seeds. It supports the corn seeds to prevent them from sliding due to gravity during detection. The outer edge plate 401 on the polypropylene bottom plate 4 cooperates with the guide sliding groove 302 of the four-corner support block 3 to realize tool-free insertion of the polypropylene bottom plate 4.

[0042] The high transparency of the polycarbonate microplate 1 of the device ensures that the fluorescent signal is not attenuated, and it is compatible with a fluorescence sorting box or a multifunctional microplate detector. The rectangular array layout of the sample placement hole 101 conforms to the reading format of a standard microplate detector (such as a 96-well / 384-well plate). The right-angled recess 301 of the four-corner support block 3 enables vertical nesting, saving storage space and being suitable for batch detection scenarios in laboratories or fields. The design of the notch area 201 and the taking and placing handle groove 202 improves the convenience of operation, especially for high-frequency use scenarios. The polypropylene bottom plate 4 is installed in a "plug-in" manner through the guide sliding groove 303 and the quick clamping piece 5, simplifying the operation process and improving the detection efficiency. The detachable polypropylene bottom plate 4 design facilitates cleaning and reduces the risk of cross-contamination;

[0043] Specifically, the guide sliding groove 302 of the four-corner support block 3 is a symmetrical structure, and the outer edge plate 401 of the polypropylene bottom plate 4 is chamfered at the corners, so that the polypropylene bottom plate 4 can be inserted or removed from the gap between any two adjacent four-corner support blocks 3, achieving multi-directional disassembly;

[0044] The guide sliding groove 302 of the four-corner support block 3 adopts a symmetrical structure, ensuring that the bottom plate 4 can be inserted from the gap between any two adjacent four-corner support blocks 3, breaking through the limitations of traditional one-way sliding rails. The bilateral symmetrical support of the guide sliding groove 302 disperses the bearing pressure of the polypropylene bottom plate 4, reduces unilateral wear, and the chamfered outer edge plate 401 reduces insertion resistance and avoids hard friction. The chamfer angle matches the sliding groove entrance, and a self-guiding inclined plane is formed at an angle of 30-45 degrees, achieving "blind insertion" fault tolerance.

[0045] Specifically, the quick clamping piece 5 includes:

[0046] The extension spring 501 is arranged in the polypropylene bottom plate 4, with the top end fixed in the placement groove 402;

[0047] The lifting movable plate 502 is connected to the bottom end of the extension spring 501, and the lifting movable plate 502 is constrained in vertical movement by the limiting groove 403;

[0048] The arc-shaped clamping head 503 is fixed to the lower end of the lifting movable plate 502, with the distal end matched and locked with the arc-shaped clamping groove 404 on the outer edge plate 401;

[0049] At the beginning, the telescopic spring 501 is in a free state, the end of the arc-shaped clamping joint 503 is lower than the upper surface of the outer edge plate 401, the outer edge plate 401 is not inserted into the detection disc, and due to the displacement constraint of the limiting groove 403, the upper end surface of the outer edge plate 401 and the arc surface of the arc-shaped clamping joint 503 are at the same height, avoiding that the telescopic amount of the arc-shaped clamping joint 503 is too large, causing the outer edge plate 401 to be difficult to insert, when the outer edge plate 401 slides into the guide sliding groove 302, the upper wall surface of the outer edge plate 401 presses the arc-shaped clamping joint 503 upward, compressing the telescopic spring 501, the outer edge plate 401 moves along the guide sliding groove 302, until the arc-shaped clamping joints 503 at the four corners are clamped in the corresponding arc-shaped clamping grooves 404, the matching locking is completed, and due to the setting of the arc-shaped clamping joint 503 and the arc-shaped clamping groove 404, the polypropylene bottom plate 4 can be directly disassembled without tools under the condition of ensuring the stability of the polypropylene bottom plate 4;

[0050] Specifically, the inner wall of the guide sliding groove 302 is sprayed with a polytetrafluoroethylene wear-resistant coating, and the thickness is 0.5-1.2mm;

[0051] Spraying polytetrafluoroethylene coating on the inner wall of the guide sliding groove 302 can significantly improve the durability and smoothness of the operation of the detection disc;

[0052] Specifically, the lower end surface of the polypropylene bottom plate 4 is 0.5-2mm higher than the lower end surface of the four-corner supporting block 3, and the bottom surface of the polypropylene bottom plate 4 is provided with cross-symmetrically distributed disassembly handle grooves one 405 and two 406 for assisting the taking and placing operation;

[0053] The lower end surface of the polypropylene bottom plate 4 is 0.5-2mm higher than the lower end surface of the four-corner supporting block 3, avoiding direct contact of the polypropylene bottom plate 4 with the experimental bench surface or friction with other detection discs when stacked, the disassembly handle grooves one 405 are symmetrically distributed along the short axis direction of the polypropylene bottom plate 4 and located at the left and right edges of the polypropylene bottom plate 4, and the disassembly handle grooves two 406 are symmetrically distributed along the long axis direction and located at the front and rear edges of the polypropylene bottom plate 4, ensuring that the polypropylene bottom plate 4 can be conveniently taken and placed from any direction.

[0054] Working principle: The device realizes the efficiency, convenience and durability of corn seed fluorescence detection through modular design, high transparency material and intelligent clamping technology. Its multi-directional insertion, vertical stacking and quick disassembly function significantly improves the efficiency of laboratory and field detection, and is suitable for high-frequency and batch seed screening scenarios. It is composed of the following core components:

[0055] Polycarbonate microplate 1: as the core bearing component, it is used to hold corn seeds and support fluorescence detection.

[0056] Upper edge plate 2: fixed to the upper end of the polycarbonate microplate 1, providing operation convenience and structural protection.

[0057] Four corner support block 3: fixed in the lower end of the four corners of the polycarbonate microplate 1, realize the function of stacking and the bottom plate guide.

[0058] Polypropylene bottom plate 4: detachable load-bearing component, through the guide chute 302 and the quick joint 5 to realize the quick plug-in.

[0059] Quick joint 5: ensure the stability of the polypropylene bottom plate 4 after installation, support tool-free disassembly.

[0060] The specific detection process is as follows:

[0061] S1, seed loading: pour the corn seeds into the sample hole 101 of the polycarbonate microplate 1, and the excess seeds are discharged through the gap area 201.

[0062] S2, bottom plate installation: insert the polypropylene bottom plate 4 into the four corner support block 3 guide chute 302 in any direction, and the quick joint 5 is automatically locked.

[0063] S3, fluorescence detection: the polycarbonate microplate 1 is placed in the fluorescence sorter, and the high light transmittance ensures that the excitation light penetrates, and the fluorescence signal of the seeds in the hole is collected and analyzed.

[0064] S4, disassembly and cleaning: pull out the polypropylene bottom plate 4 and soak it for cleaning to avoid cross contamination between batches.

[0065] S5, stacking storage: after detection, vertically stack through the right-angle groove 301 of the four corner support block 3 to save space.

[0066] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variant thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.

[0067] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A corn seed fluorescence signal detection disk, characterized in that... ,include: The polycarbonate microporous plate (1) has a cuboid structure and several sample placement holes (101) arranged in a rectangular array are opened through the surface to hold corn seeds. The sample placement holes (101) are provided with a hydrophobic coating to reduce seed adhesion residue. The upper edge plate (2) is fixed to the upper edge of the polycarbonate microporous plate (1), and its front end is provided with a notch area (201) for discharging excess seeds. The upper edge plate (2) is provided with symmetrical handle grooves (202) on both sides. The four corner support blocks (3) are fixed at the four lower corners of the polycarbonate microporous plate (1). The lower end of the four corner support blocks (3) is provided with right-angled grooves (301) for nesting and overlapping with the four corners of the upper edge plate (2) of another detection plate. The polypropylene base plate (4) is detachably installed between the four corner support blocks (3) to support the corn seeds of the polycarbonate microporous plate (1), and is inserted through the sliding cooperation between the guide groove (302) and the outer edge plate (401), and is limited and fixed by the quick snap fastener (5).

2. The maize seed fluorescence signal detection disk according to claim 1, characterized in that: The guide groove (302) of the four corner support blocks (3) has a symmetrical structure, and the four corners of the outer edge plate (401) of the polypropylene base plate (4) are chamfered, so that the polypropylene base plate (4) can be inserted or removed from the gap between any two adjacent four corner support blocks (3) to achieve multi-directional disassembly and assembly.

3. The maize seed fluorescence signal detection disk according to claim 1, characterized in that: The quick card connector (5) includes: The top end of the telescopic spring (501) installed in the polypropylene base plate (4) is fixed in the placement groove (402); A lifting movable plate (502) is connected to the bottom end of the telescopic spring (501), and the lifting movable plate (502) is constrained to move vertically by a limiting groove (403); The arc-shaped snap connector (503) fixed to the lower end of the lifting movable plate (502) has its end matched and locked with the arc-shaped snap groove (404) on the outer edge plate (401).

4. The maize seed fluorescence signal detection disk according to claim 1, characterized in that: Furthermore, the inner wall of the guide groove (302) is coated with a polytetrafluoroethylene wear-resistant coating with a thickness of 0.5-1.2 mm.

5. The maize seed fluorescence signal detection disk according to claim 1, characterized in that: The lower end face of the polypropylene base plate (4) is 0.5-2mm higher than the lower end face of the four corner support blocks (3), and the bottom surface of the polypropylene base plate (4) is provided with a cross-shaped symmetrically distributed disassembly handle groove one (405) and disassembly handle groove two (406) to assist in the picking and placing operation.

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