Enzymolysis-membrane filtration circulating device

Abstract

The utility model provides an enzymolysis-membrane filtration circulating device, particularly relates to the technical field of biological catalytic separation, and aims to solve the technical problem that the process of cleaning accumulated membrane pollution by regularly opening a reaction tank in the use process of an immobilized enzyme membrane reactor is very inconvenient. The device comprises a controller as well as a substrate solution tank, an immobilized enzyme reaction mechanism, a membrane separation mechanism and a product collection tank which are sequentially connected through pipelines, a pump body is arranged between the substrate solution tank and the immobilized enzyme reaction mechanism, and the front end of the membrane separation mechanism is connected with a waste liquid collection tank through a pipeline; a three-way reversing valve a is arranged between the membrane separation mechanism and the product collection tank, and one end of the three-way reversing valve a is connected with a backwashing mechanism. According to the device, through the arrangement of the backwashing mechanism and the three-way reversing valve, switching between a production mode and a membrane cleaning mode can be realized, and membrane pollution can be cleaned without opening the reaction tank through the backwashing mechanism in the membrane cleaning mode.

Description

Technical Field

[0001] This utility model relates to the field of biocatalytic separation technology, and more specifically, to an enzymatic hydrolysis-membrane filtration cycle device. Background Technology

[0002] Enzyme hydrolysis-membrane technology is a process technology that integrates enzymatic hydrolysis reactions with membrane separation technology. Its core lies in leveraging the selective separation function of membranes to dynamically separate target products while retaining enzyme activity during enzymatic hydrolysis, thereby achieving efficient and controllable biocatalysis and separation. Existing enzyme hydrolysis-membrane devices include immobilized enzyme membrane reactors, free enzyme membrane reactors, cross-flow enzyme membrane reactors, membrane bioreactors, and stirred tank enzyme membrane reactors. Among these, immobilized enzyme membrane reactors offer advantages such as recyclable enzymes, membrane materials resistant to high-temperature corrosion suitable for continuous industrial production, and reduced wastewater discharge. However, immobilized enzyme membrane reactors require periodic opening of the reaction tank for cleaning accumulated membrane fouling, which is inconvenient. Therefore, an enzyme hydrolysis-membrane filtration circulation device is designed to clean membrane fouling without opening the reaction tank. Utility Model Content

[0003] The purpose of this invention is to provide an enzymatic hydrolysis-membrane filtration circulation device that can clean membrane fouling.

[0004] The embodiments of this utility model are achieved through the following technical solutions:

[0005] An enzymatic hydrolysis-membrane filtration cycle device includes a controller and a substrate solution tank, an immobilized enzyme reaction mechanism, a membrane separation mechanism, and a product collection tank connected in sequence via pipelines. A pump body electrically connected to the controller is provided between the substrate solution tank and the immobilized enzyme reaction mechanism. A waste liquid collection tank is connected to the front end of the membrane separation mechanism via a pipeline. A three-way reversing valve (a) is provided between the membrane separation mechanism and the product collection tank. One end of the three-way reversing valve (a) is connected to a backwashing mechanism electrically connected to the controller.

[0006] Preferably, a circulating pump electrically connected to the controller is provided between the immobilized enzyme reaction mechanism and the membrane separation mechanism, and a b three-way reversing valve is provided between the membrane separation mechanism and the waste liquid collection tank. One end of the b three-way reversing valve is connected to the pipeline between the substrate solution tank and the immobilized enzyme reaction mechanism through a pipeline.

[0007] Preferably, the backwashing mechanism includes a high-pressure pump connected to the three-way reversing valve a, and one end of the high-pressure pump is connected to a backwash liquid tank.

[0008] Preferably, the immobilized enzyme reaction mechanism includes a first tank, the interior of which is equipped with a magnetic nanoparticle carrier, and the exterior of which is surrounded by a magnet.

[0009] Preferably, the inner side of the first tank is provided with a temperature sensor electrically connected to the controller, and the first tank is embedded with an electric heating wire and a cooling water pipe electrically connected to the controller.

[0010] Preferably, the membrane separation mechanism includes a second tank, inside which are arranged a plurality of vertically stacked flat membranes. The inner side wall of the second tank is provided with a first guide plate communicating with an inlet pipe and a second guide plate communicating with an outlet pipe. The first guide plate and the second guide plate are both arranged with a plurality of grooves in the vertical direction, and the grooves are in contact with the plane of the flat membrane on the same horizontal plane.

[0011] Preferably, the inner side of the second tank is also provided with a plurality of piezoresistive pressure transmitters electrically connected to the controller, and the piezoresistive pressure transmitters are respectively installed near the first guide plate and the second guide plate.

[0012] Preferably, a flow meter electrically connected to the controller is provided between the membrane separation mechanism and the three-way reversing valve a.

[0013] Preferably, the outer side of the second tank is also provided with a plurality of ultrasonic generators electrically connected to the controller.

[0014] The technical solution of this utility model embodiment has at least the following advantages and beneficial effects:

[0015] 1. This device, through the backwashing mechanism and the three-way reversing valve, can switch between production mode and membrane cleaning mode. In membrane cleaning mode, the backwashing structure can clean membrane fouling without opening the reaction tank.

[0016] 2. In this device, the macromolecular products or unreacted substrates filtered and retained by the membrane can enter the pipeline between the substrate solution tank and the immobilized enzyme reaction mechanism under the action of the circulation pump, and then re-enter the immobilized enzyme reaction mechanism through the pump body for complete re-reaction, realizing the recycling of unreacted raw materials, thereby improving the utilization rate of raw materials and saving production costs. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of an enzymatic hydrolysis-membrane filtration circulation device according to the present invention;

[0019] Figure 2 This is a schematic diagram of the internal structure of the immobilized enzyme reaction mechanism in this utility model;

[0020] Figure 3 This is a schematic diagram of the internal structure of the membrane separation mechanism in this utility model.

[0021] Icons: 1. Substrate solution tank; 2. Immobilized enzyme reaction mechanism; 3. Membrane separation mechanism; 4. Product collection tank; 5. Pump body; 6. Waste liquid collection tank; 7. a. Three-way reversing valve; 8. Circulation pump; 9. b. Three-way reversing valve; 10. High-pressure pump; 11. Backwash liquid tank; 12. First tank; 13. Magnetic nanoparticle carrier; 14. Magnet; 15. Temperature sensor; 16. Electric heating wire; 17. Cooling water pipe; 18. Second tank; 19. Flat sheet membrane; 20. First guide plate; 21. Second guide plate; 22. Groove; 23. Piezoresistive pressure transmitter; 24. Flow meter; 25. Ultrasonic generator; 26. Check valve. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0023] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0024] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0025] In the description of this utility model, it should be noted that if terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" appear to indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0026] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0027] An enzymatic hydrolysis-membrane filtration cycle device, in some embodiments, such as Figures 1-3 As shown, the device includes a controller and a substrate solution tank 1, an immobilized enzyme reaction mechanism 2, a membrane separation mechanism 3, and a product collection tank 4 connected in sequence via pipelines. A pump body 5 electrically connected to the controller is provided between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2. The front end of the membrane separation mechanism 3 is connected to a waste liquid collection tank 6 via a pipeline. A three-way reversing valve 7 is provided between the membrane separation mechanism 3 and the product collection tank 4. One end of the three-way reversing valve 7 is connected to a backwashing mechanism electrically connected to the controller. The controller can be a Siemens S7-1200 industrial PLC or an ARM Cortex-M series embedded controller. The pump body 5 is a peristaltic pump, and the three-way reversing valve 7 is an L-type reversing valve.

[0028] In practical use, the solution in the substrate solution tank 1 enters the immobilized enzyme reaction mechanism 2 under the action of the pump body 5 for reaction. Since the enzyme is immobilized on the carrier in the immobilized enzyme reaction mechanism 2, the enzyme can be repeatedly recycled, improving stability and potentially reducing enzyme loss during the reaction. After the reaction, it enters the membrane separation mechanism 3, where it is filtered and large molecular products or unreacted substrates are retained, and the product enters the product collection tank 4 for collection. When it is necessary to clean the membrane in the membrane separation mechanism 3 that has become contaminated, the three-way reversing valve 7 is switched to connect the backwashing mechanism to the membrane separation mechanism 3, while disconnecting the membrane separation mechanism 3 from the product collection tank 4. At this time, the controller controls the backwashing mechanism to start, thereby backwashing the membrane surface. The waste liquid from the backwashing enters the waste liquid collection tank 6 through the pipeline at the front end of the membrane separation mechanism 3 for centralized collection. This device, through the backwashing mechanism and the three-way reversing valve, can switch between production mode and membrane cleaning mode. In membrane cleaning mode, the backwashing structure can clean membrane fouling without opening the reaction tank.

[0029] In some embodiments, such as Figures 1-2 As shown, a circulating pump 8 electrically connected to the controller is provided between the immobilized enzyme reaction mechanism 2 and the membrane separation mechanism 3. A three-way reversing valve 9 (b) is provided between the membrane separation mechanism 3 and the waste liquid collection tank 6. One end of the three-way reversing valve 9 (b) is connected to the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2 through a pipeline. The three-way reversing valve 7 (a) is an L-type reversing valve.

[0030] In practical use, when the device is in production mode, the b three-way reversing valve 9 controls the connection between the pipeline at the front end of the membrane separation mechanism 3 and the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2. This allows the macromolecular products or unreacted substrates filtered and retained by the membrane to enter the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2 under the action of the circulating pump 8, and then re-enter the immobilized enzyme reaction mechanism 2 through the pump body 5 for a complete reaction. This achieves the recycling of unreacted raw materials, thereby improving the utilization rate of raw materials and saving production costs. When in membrane cleaning mode, the b three-way reversing valve 9 can control the disconnection of the pipeline at the front end of the membrane separation mechanism 3 from the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2 and connect it to the waste liquid collection tank 6.

[0031] It is worth mentioning that check valves 26 are provided between the circulating pump 8 and the membrane separation mechanism 3, and between the membrane separation mechanism 3 and the bracket of the b three-way reversing valve 9, to prevent waste liquid from flowing back.

[0032] In some embodiments, such as Figures 1-2 As shown, the backwashing mechanism includes a high-pressure pump 10 connected to the three-way reversing valve 7, and one end of the high-pressure pump 10 is connected to a backwash liquid tank 11.

[0033] In practical use, the controller starts the high-pressure pump 10 to backwash the cleaning agent and liquid in the backwash tank 11 into the membrane separation mechanism 3 to backwash and clean the membrane. The waste liquid after backwashing enters the waste liquid collection tank 6 through the pipeline at the front end of the separation mechanism via the three-way reversing valve 9.

[0034] In some embodiments, such as Figures 1-2 As shown, the immobilized enzyme reaction mechanism 2 includes a first tank 12, inside which a magnetic nanoparticle carrier 13 is installed, and a magnet 14 is arranged around the outside of the first tank 12, the magnet 14 being electrically connected to the controller.

[0035] In practical use, the surface of the magnetic nanoparticle carrier 13 is modified with amino or carboxyl groups, and the enzyme is fixed by covalent bonds. By controlling the periodic opening and closing of the magnet 14 on the outside of the first container 12, the magnetic nanoparticle carrier 13 is suspended (enhanced mass transfer) or settled (prevents blockage) in the first container 12, which facilitates the fixation of the magnetic nanoparticle carrier 13.

[0036] In some embodiments, such as Figures 1-2 As shown, the inner side of the first tank 12 is provided with a temperature sensor 15 that is electrically connected to the controller. The first tank 12 is embedded with an electric heating wire 16 and a cooling water pipe 17 that are electrically connected to the controller. The cooling water pipe 17 is connected to an external liquid supply system.

[0037] In practical use, the temperature inside the immobilized enzyme reactor can be adjusted and controlled by the feedback from the temperature sensor 15 and by controlling the electric heating wire 16 and the cooling water pipe 17.

[0038] In some embodiments, such as Figures 1-2 As shown, the membrane separation mechanism 3 includes a second tank 18, inside which are arranged a plurality of vertically stacked flat membranes 19. The inner sidewall of the second tank 18 is provided with a first guide plate 20 connected to the liquid inlet pipe and a second guide plate 21 connected to the liquid outlet pipe. The first guide plate 20 and the second guide plate 21 are both arranged with a plurality of grooves 22 in the vertical direction. The grooves 22 are in contact with the plane of the flat membrane 19 on the same horizontal plane.

[0039] In practical use, the first guide plate 20 and the second guide plate 21 make the feed liquid flow parallel to the membrane plane when it flows on the flat sheet membrane 19, thereby forming a cross-flow filtration channel, which helps to reduce the pollution and clogging of the membrane surface. Furthermore, the shear force generated when the feed liquid flows through the membrane surface can effectively remove particles from the membrane surface and prevent particles from accumulating on the membrane surface to form a thick pollution layer.

[0040] In some embodiments, such as Figures 1-2 As shown, the inner side of the second tank 18 is also provided with a plurality of piezoresistive pressure transmitters 23 that are electrically connected to the controller. The piezoresistive pressure transmitters 23 are respectively installed near the first guide plate 20 and the second guide plate 21.

[0041] In practical use, the piezoresistive pressure transmitters 23, which are respectively installed near the first guide plate 20 and the second guide plate 21, can detect the actual pressure difference between the feed side and the permeate side of the flat sheet membrane 19. When the pressure difference reaches the corresponding value, it proves that the membrane fouling has reached the corresponding state. At this time, the device needs to be switched to the membrane cleaning mode to clean the membrane.

[0042] In some embodiments, such as Figures 1-2 As shown, a flow meter 24 electrically connected to the controller is provided between the membrane separation mechanism 3 and the three-way reversing valve 7.

[0043] In practical use, the flow rate of the product liquid is detected by the flow meter 24, and the monitoring of the piezoresistive pressure transmitter 23 is combined to improve the accuracy of membrane fouling monitoring.

[0044] In some embodiments, such as Figures 1-2 As shown, the outer side of the second tank 18 is also provided with a plurality of ultrasonic generators 25 that are electrically connected to the controller.

[0045] In practical use, efficient cleaning is achieved through the cavitation effect of the ultrasonic generator combined with the cleaning agent and backflushing fluid of the backflushing mechanism.

[0046] In some other embodiments, the inner side of the second tank 18 is also provided with a pH probe electrically connected to the controller, so as to monitor the pH value in the second tank 18 and facilitate the adjustment and adaptation of the cleaning agent added to the backwash liquid tank 11.

[0047] The specific operating principle of this device is as follows:

[0048] In the production mode, the solution in substrate solution tank 1 enters the immobilized enzyme reaction mechanism 2 under the action of pump 5 for reaction. Since the enzyme is immobilized on the carrier in the immobilized enzyme reaction mechanism 2, the enzyme can be repeatedly recycled, improving stability and potentially reducing enzyme loss during the reaction. After the reaction, it enters the membrane separation mechanism 3, where it filters and retains macromolecular products or unreacted substrates, and the product enters the product collection tank 4 for collection. Furthermore, the three-way reversing valve 9 controls the connection between the pipeline at the front end of the membrane separation mechanism 3 and the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2, so that the membrane-filtered and retained macromolecular products or unreacted substrates can enter the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction mechanism 2 under the action of the circulation pump 8, and then re-enter the immobilized enzyme reaction mechanism 2 through pump 5 for complete reaction again, realizing the recycling of unreacted raw materials, thereby improving the utilization rate of raw materials and saving production costs.

[0049] When cleaning of the contaminated membrane in membrane separation unit 3 is required, switch the a three-way reversing valve 7 to connect the backwashing mechanism to the membrane separation unit 3, while disconnecting the membrane separation unit 3 from the product collection tank 4. Also, switch the b three-way reversing valve 9 to disconnect the pipeline at the front end of the membrane separation unit 3 from the pipeline between the substrate solution tank 1 and the immobilized enzyme reaction unit 2, and connect it to the waste liquid collection tank 6, thus switching to membrane cleaning mode. The controller then activates the backwashing mechanism to backwash the membrane surface. The backwashed waste liquid enters the waste liquid collection tank 6 through the pipeline at the front end of the membrane separation unit 3 for centralized collection. This device, through the backwashing mechanism and the three-way reversing valve, enables switching between production mode and membrane cleaning mode. Furthermore, in membrane cleaning mode, the backwashing structure allows for cleaning of the membrane without opening the reaction tank.

[0050] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An enzymatic-membrane filtration circulation apparatus, characterized by: The application relates to a kind of membrane separation systems for immobilized enzyme reaction, including controller and by pipeline sequentially connected substrate solution tank (1), immobilized enzyme reaction mechanism (2), membrane separation mechanism (3) and product collection tank (4), pump body (5) is equipped between the substrate solution tank (1) and the immobilized enzyme reaction mechanism (2) and is electrically connected with the controller, the front end of the membrane separation mechanism (3) is connected with waste liquid collection tank (6) by pipeline;Between the membrane separation mechanism (3) and the product collection tank (4), a three-way valve (7) is equipped, one end of the a three-way valve (7) is connected with backwashing mechanism and is electrically connected with the controller.

2. The enzyme-membrane filtration circulation device according to claim 1, wherein: Between the immobilized enzyme reaction mechanism (2) and the membrane separation mechanism (3), circulating pump (8) is equipped and is electrically connected with the controller, between the membrane separation mechanism (3) and the waste liquid collection tank (6), b three-way valve (9) is equipped, one end of the b three-way valve (9) is connected with the pipeline between the substrate solution tank (1) and the immobilized enzyme reaction mechanism (2) by pipeline.

3. The enzyme-membrane filtration circulation device according to claim 2, wherein: The backwashing mechanism includes high-pressure pump (10) connected with the a three-way valve (7), one end of the high-pressure pump (10) is connected with backwashing liquid tank (11).

4. The enzyme-membrane filtration circulation device according to claim 1, wherein: The immobilized enzyme reaction mechanism (2) includes first tank body (12), magnetic nanoparticles carrier (13) is installed in the inside of the first tank body (12), and magnet (14) is arranged on the outside of the first tank body (12).

5. The enzyme-membrane filtration circulation device according to claim 4, wherein: Temperature sensor (15) is arranged on the inside of the first tank body (12) and is electrically connected with the controller, and electric heating wire (16) and cooling water pipeline (17) are embedded in the first tank body (12) and are electrically connected with the controller.

6. The enzyme-membrane filtration circulation device according to claim 1, wherein: The membrane separation mechanism (3) includes second tank body (18), a plurality of vertical stacked flat sheet membranes (19) are arranged in the second tank body (18), first flow guide plate (20) is arranged on the inner wall of the second tank body (18) and is communicated with liquid inlet pipeline, and second flow guide plate (21) is arranged on the inner wall of the second tank body (18) and is communicated with liquid outlet pipeline, a plurality of grooves (22) are arranged on the first flow guide plate (20) and the second flow guide plate (21) in vertical direction, and the grooves (22) are connected with the flat sheet membranes (19) on the same horizontal plane.

7. The enzyme-membrane filtration circulation device according to claim 6, wherein: A plurality of piezoresistive pressure transmitters (23) are arranged on the inside of the second tank body (18) and are electrically connected with the controller, and the piezoresistive pressure transmitters (23) are respectively arranged near the first flow guide plate (20) and the second flow guide plate (21).

8. The enzyme-membrane filtration circulation device according to claim 1, wherein: Flow meter (24) is arranged between the membrane separation mechanism (3) and the a three-way valve (7) and is electrically connected with the controller.

9. The enzyme-membrane filtration circulation device according to claim 7, wherein: A plurality of ultrasonic generators (25) are arranged on the outside of the second tank body and are electrically connected with the controller.

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