Convenient observation culture box for symbiotic control experiment of rhizobium

Abstract

The utility model provides a convenient observation culture box for a symbiotic control experiment of rhizobium, which relates to the technical field of observation culture boxes and comprises a box body, sampling components are mounted at corners of the left side of the box body close to the top end and the front side, and culture bins are symmetrically arranged in the box body close to the left side and the right side. Sampling holes are formed in the positions, close to the four corners, of the left side and the right side of the box body, a water receiving assembly is installed at the position, close to the bottom, of the interior of the box body, the sampling assembly comprises a fixing sleeve, the fixing sleeve is inserted into soil of a culture bin, the sampling sleeve is pulled out of the fixing sleeve, and soil observation and nitrogen fixing capacity detection are conducted. The fixed sleeve left in the sampling hole can prevent the soil layer from collapsing, the sampling sleeve is re-inserted into the fixed sleeve, and the fixed sleeve is synchronously pulled out while the push rod is pushed, so that the soil resetting is completed, the observation and detection convenience is greatly improved, and the damage to the whole data of the experiment is effectively reduced.

Description

Technical Field

[0001] This utility model relates to the field of observation culture box technology, and in particular to a convenient observation culture box for rhizobium symbiotic control experiments. Background Technology

[0002] Rhizobia are important symbiotic bacteria for legumes in reclaimed areas. Rhizobia are Gram-negative bacteria that can form root nodules with legumes and reduce atmospheric nitrogen to ammonia to provide nutrients for the plants. Rhizobia are mainly found in the soil and can form root nodule structures with the roots of legumes. Through nitrogen fixation, they provide nitrogen fertilizer to the plants, thereby promoting plant growth and development. The rhizobia symbiotic control experiment is an experiment that compares the growth status of plants inoculated with rhizobia and those not inoculated with rhizobia. It aims to explore the symbiotic relationship between rhizobia and plants and their effects on plant growth and nitrogen fixation capacity. During the rhizobia symbiotic control experiment, it is necessary to regularly test the nitrogen fixation capacity inside the soil.

[0003] Commonly used culture boxes for rhizobium symbiosis control experiments have a relatively simple structure. The box walls usually have sampling holes. When sampling the soil, a sampling tube needs to be inserted through the sampling hole to collect the soil sample. However, this sampling method has certain drawbacks. The soil in the culture box is relatively loose, and after the sampling tube is removed, the soil layer is prone to collapse, which makes it impossible to reposition the soil after testing. Due to the rigor of the experiment, the change in the soil layer is not conducive to the overall data collection of the experiment. Therefore, we propose a convenient observation culture box for rhizobium symbiosis control experiments. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies. Common culture boxes used in rhizobium symbiotic control experiments have relatively simple structures, with sampling holes usually provided on the box walls. When sampling the soil, a sampling tube needs to be inserted through the sampling hole to collect the soil sample. However, this sampling method has certain drawbacks. The soil in the culture box is relatively loose, and after the sampling tube is removed, the soil layer is prone to collapse, making it impossible to reposition the tested soil. Due to the rigor of the experiment, the change in the soil layer is not conducive to the overall data collection of the experiment.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A convenient observation culture box for a rhizobium symbiotic control experiment includes a box body, and a sampling component is installed at the corner of the left side of the box body near the top and the front.

[0007] The box body has symmetrically arranged culture chambers near the left and right sides inside. The box body has sampling holes near the four corners on the left and right sides. The box body has a water receiving component installed near the bottom inside.

[0008] The sampling assembly includes a fixed sleeve, which is inserted into one of the sampling holes on the left side. Connecting blocks are symmetrically connected to the front and back of the fixed sleeve and to the left of the box. A support rod is installed between the right side of the connecting block and the box. A sampling sleeve is slidably connected inside the fixed sleeve. A sampling port is opened on the periphery of the sampling sleeve. A push rod is slidably connected inside the sampling sleeve.

[0009] As a preferred embodiment of this utility model, the front of the box body has an observation window corresponding to the culture chamber.

[0010] The technical advantage of adopting the above-mentioned further scheme is that it facilitates real-time observation of plant roots through the observation window, thereby improving the convenience of observation.

[0011] As a preferred embodiment of this utility model, the bottom of the culture chamber is provided with a plurality of equally spaced perforated holes, and a filter screen is installed inside the perforated holes.

[0012] The technical advantage of adopting the above-mentioned further solution is that excess irrigation water in the culture chamber can be drained through the leakage holes, thus improving its practicality.

[0013] As a preferred embodiment of this utility model, a sealing cap is inserted into the outer side of the sampling hole, and a sealing ring is adhered to the outer periphery of the sealing cap at the contact point with the sampling hole.

[0014] The technical effect of adopting the above-mentioned further solution is that the use of sealing caps and sealing rings can prevent soil and irrigation water from overflowing, thereby improving the stability of use.

[0015] As a preferred embodiment of this utility model, the water receiving component includes a water receiving basin, and sliding strips are symmetrically welded to the left and right sides of the water receiving basin near the top, and the sliding strips are slidably connected to the box body.

[0016] The technical advantage of adopting the above-mentioned further solution is that excess irrigation water can be received by the water receiving basin, preventing pollution of the working environment and improving ease of use.

[0017] As a preferred embodiment of this utility model, the outer periphery of one end of the fixed sleeve extending into the culture chamber is a conical structure, and the inner opening is a radial structure.

[0018] The technical advantages of adopting the above-mentioned further solution are: the conical structure of the outer periphery of the fixed sleeve makes it easier and less strenuous to insert the fixed sleeve into the soil; the radial structure of the opening inside the fixed sleeve facilitates the entry of soil into the sampling sleeve, thereby improving the stability of use.

[0019] As a preferred embodiment of this utility model, a threaded groove is provided on the outer periphery of the sampling sleeve near the left end, and the sampling sleeve is threadedly connected to the fixed sleeve.

[0020] The technical effect of adopting the above-mentioned further solution is that the threaded connection between the sampling sleeve and the fixed sleeve can fix the two together, preventing displacement during the insertion into the soil and improving the stability of use.

[0021] As a preferred embodiment of this utility model, a push block is installed at one end of the push rod near the inside of the sampling sleeve.

[0022] The technical effect of adopting the above-mentioned further solution is that by pushing the push rod, it can drive the push block to push out and reset the soil inside the sampling sleeve, thereby improving the ease of use.

[0023] Compared with the prior art, the beneficial effects of this utility model are:

[0024] In this invention, the design of the box and sampling components allows the fixing sleeve to be inserted into the soil of the culture chamber through the sampling hole. During the process, the soil cut by the fixing sleeve enters the interior of the sampling sleeve. Then, the sampling sleeve is rotated to release the threaded fixing state from the fixing sleeve and is pulled out from the interior of the fixing sleeve. This allows for observation and nitrogen fixation capacity testing of the corresponding soil. The fixing sleeve remaining in the sampling hole prevents soil collapse. After the test is completed, the sampling sleeve is reinserted into the fixing sleeve, and the fixing sleeve is simultaneously pulled out while the push rod is pushed, thus completing the soil reset. Compared with traditional culture boxes, this invention greatly improves the convenience of observation and testing and effectively reduces the damage to the overall experimental data. Attached Figure Description

[0025] Figure 1 A schematic diagram of the overall structure of a convenient observation and culture box for a rhizobium symbiotic control experiment provided by this utility model;

[0026] Figure 2 Anatomical diagram of the overall front structure of a convenient observation culture box for a rhizobium symbiotic control experiment provided by this utility model;

[0027] Figure 3 Anatomical diagram of the top structure of the sampling component of a convenient observation culture box for a rhizobium symbiotic control experiment provided by this utility model;

[0028] Figure 4 A schematic diagram of the top structure of the sampling component of a convenient observation culture box for a rhizobium symbiotic control experiment provided by this utility model.

[0029] Legend: 1. Box body; 101. Culture chamber; 1011. Leakage hole; 102. Sampling hole; 1021. Sealing cover; 103. Water receiving assembly; 1031. Water receiving basin; 1032. Sliding bar; 104. Observation window; 2. Sampling assembly; 201. Fixing sleeve; 202. Connecting block; 203. Support rod; 204. Sampling sleeve; 205. Sampling port; 206. Push rod; 2061. Push block. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0031] To facilitate understanding of this utility model, a more comprehensive description of this utility model will be provided below with reference to relevant embodiments, and several embodiments of this utility model will be given. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of this utility model more thorough and complete.

[0032] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0034] Example 1

[0035] like Figure 1-4As shown, this utility model provides a technical solution: a convenient observation culture box for a rhizobium symbiotic control experiment, including a box body 1. A sampling component 2 is installed on the left side near the top and the corner of the front of the box body 1. Culture chambers 101 are symmetrically opened on the left and right sides of the box body 1. Sampling holes 102 are opened on the left and right sides near the four corners of the box body 1. A water receiving component 103 is installed on the inside of the box body 1 near the bottom. The sampling component 2 includes a fixing sleeve 201, which is inserted into one of the sampling holes 102 on the left side. The fixing sleeve 201 is surrounded by a fixed sleeve 201. The front and back sides of the box 1 are symmetrically connected with connecting blocks 202. A support rod 203 is installed between the right side of the connecting block 202 and the box 1. A sampling sleeve 204 is slidably connected inside the fixing sleeve 201. A sampling port 205 is opened on the periphery of the sampling sleeve 204. Through the sampling port 205, the soil can be observed and nitrogen fixation capacity can be tested without removing most of the soil from inside the sampling sleeve 204, which facilitates subsequent soil restoration operations and improves the convenience of observation. A push rod 206 is slidably connected inside the sampling sleeve 204.

[0036] Example 2

[0037] like Figure 1-4As shown, the front of the box 1, corresponding to the cultivation chamber 101, has an observation window 104. The observation window 104 facilitates real-time observation of the plant roots, improving observation convenience. The bottom of the cultivation chamber 101 has several evenly spaced drainage holes 1011. A filter screen is installed inside each drainage hole 1011, allowing excess irrigation water to drain from the cultivation chamber 101, improving practicality. A sealing cap 1021 is inserted into the outside of the sampling hole 102. A sealing ring is adhered to the outer edge of the sealing cap 1021 where it contacts the sampling hole 102. The use of the sealing cap 1021 and the sealing ring prevents soil and irrigation water from overflowing, improving operational stability. The water receiving component 103 includes a water receiving basin 1031. Sliding strips 1032 are symmetrically welded to the left and right sides of the water receiving basin 1031 near the top. The sliding strips 1032 are slidably connected to the box 1. The water receiving basin 1031 can collect excess irrigation water, preventing contamination of the plant. To improve the working environment and ease of use, the outer periphery of the fixed sleeve 201 extending into the culture chamber 101 is tapered, and the inner opening is radial. The tapered structure of the fixed sleeve 201 makes it easier and less strenuous to insert it into the soil. The radial structure of the inner opening of the fixed sleeve 201 facilitates soil entry into the sampling sleeve 204, improving stability. The outer periphery of the sampling sleeve 204 near the left end has a threaded groove, and the sampling sleeve 204 is threadedly connected to the fixed sleeve 201. This threaded connection fixes the two sleeves together, preventing displacement during soil insertion and improving stability. A push block 2061 is installed at the end of the push rod 206 near the inside of the sampling sleeve 204. By pushing the push rod 206, the push block 2061 can be used to push the soil out and reset the sampling sleeve 204, improving ease of use.

[0038] The workflow of this invention is as follows: When sampling and testing the soil nitrogen fixation capacity of a convenient observation culture box for a rhizobium symbiotic control experiment, firstly, rotate the sampling sleeve 204 to fix it with the fixed sleeve 201 by threads, open the corresponding sealing cover 1021, and insert the fixed sleeve 201 into the corresponding sampling hole 102. During this process, as the fixed sleeve 201 extends, the cut soil will enter the interior of the sampling sleeve 204. Then, reverse the sampling sleeve 204 to release the threaded fixation from the fixed sleeve 201, and pull the sampling sleeve 204 out of the fixed sleeve 201. The soil can then be observed and sampled through the sampling port 205. After the test is completed, reinsert the sampling sleeve 204 into the fixed sleeve 201, push the push rod 206 to drive the push block 2061 to slowly push out the soil, and simultaneously pull out the fixed sleeve 201 to complete the soil reset. Compared with traditional culture boxes, this invention greatly improves the convenience of observation and testing and effectively reduces the damage to the overall experimental data.

[0039] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A convenient observation culture box for rhizobium symbiosis control experiment, comprising a box body (1), characterized in that: The sampling assembly (2) is installed on the left side of the box body (1) near the top end and the corner of the front face; The box body (1) is symmetrically provided with a culture bin (101) near the left and right sides, the box body (1) is provided with a sampling hole (102) near the four corners, and the box body (1) is provided with a water receiving assembly (103) near the bottom; The sampling assembly (2) comprises a fixed sleeve (201), the fixed sleeve (201) is inserted into one of the sampling holes (102) on the left side, the front face and the back face of the fixed sleeve (201) are connected with the left side of the box body (1), the right side of the connecting block (202) is connected with the box body (1), the support rod (203) is installed between the connecting block (202) and the box body (1), the sampling sleeve (204) is slidably connected in the fixed sleeve (201), the sampling sleeve (204) is provided with a sampling port (205) on the periphery, and the push rod (206) is slidably connected in the sampling sleeve (204).

2. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The box body (1) is provided with an observation window (104) corresponding to the culture bin (101) on the front face.

3. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The inner bottom of the culture bin (101) is uniformly provided with a plurality of leak holes (1011) penetrating the bottom, and the leak holes (1011) are provided with filter screens.

4. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The outer side of the sampling hole (102) is inserted with a sealing cover (1021), and the sealing ring is bonded on the contact between the outer periphery of the sealing cover (1021) and the sampling hole (102).

5. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The water receiving assembly (103) comprises a water receiving basin (1031), and the water receiving basin (1031) is symmetrically welded with a sliding strip (1032) near the top end on the left and right sides, and the sliding strip (1032) is slidably connected with the box body (1).

6. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The periphery of one end of the fixed sleeve (201) extending into the culture bin (101) is a conical structure, and the inner opening is a radial structure.

7. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The periphery of the sampling sleeve (204) near the left end is provided with a threaded groove, and the sampling sleeve (204) is threadedly connected with the fixed sleeve (201).

8. The convenient observation culture box for rhizobium symbiosis control experiment according to claim 1, characterized in that: The push rod (206) is provided with a push block (2061) at one end inside the sampling sleeve (204).

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