Centrifugal equipment for biological cell separation
By introducing an automatic separation system with density sensors and solenoid valves into centrifuge equipment, combined with temperature control of servo motors and heaters, the problem of low separation efficiency of cell fluid and cell tissue has been solved, achieving efficient automatic separation and temperature-appropriate biological cell separation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-26
- Publication Date
- 2026-04-03
AI Technical Summary
After the existing centrifugation equipment has finished its work, the separation of cell fluid and cell tissue requires manual operation, resulting in low separation efficiency.
A centrifuge device for biological cell separation was designed, comprising a centrifugation component and a temperature transfer component. It automatically separates cell fluid and cell material using a density sensor and a solenoid valve, and improves separation efficiency by combining a servo motor and a heater to regulate the temperature.
It achieves separation without manual intervention, significantly improves the efficiency of biological cell separation, and creates a suitable temperature environment to enhance the separation effect.
Smart Images
Figure CN224072262U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell separation technology, specifically a centrifuge device for biological cell separation. Background Technology
[0002] Cell separation technology refers to the technique of separating different types of cells or specific cellular components from tissues or cell populations to obtain a single type of cell or specific cellular component. It typically uses centrifugation equipment, taking advantage of the differences in sedimentation rates of various cells or cellular components at different centrifugation speeds. It is commonly used to separate organelles such as the cell nucleus and mitochondria.
[0003] Centrifuges are devices that use centrifugal force to separate and precipitate different components in a mixture. They are separated based on the difference in sedimentation velocity of different cells or cell components in a centrifugal force field.
[0004] However, in actual use, the centrifuges often only separate the cells in the test tube into layers after the centrifuge has finished working, and lack a device for separating cell fluid and cell tissue. The separation needs to be done manually afterward, which makes the separation efficiency low. In view of this, we propose a centrifuge for biological cell separation. Utility Model Content
[0005] The purpose of this invention is to provide a centrifuge device for biological cell separation, so as to solve the problems mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution:
[0007] A centrifuge for biological cell separation includes an operating table, a housing fixedly installed at the center of the operating table, an organic door hingedly installed inside the housing, a control console fixedly installed on the upper surface of the top of the operating table, a servo motor installed on the lower surface of the bottom of the operating table, an organic cover hingedly installed on the top of the housing, and a centrifugation assembly disposed inside the housing, the centrifugation assembly comprising:
[0008] A centrifuge tank is provided inside the casing. A rotating shaft is fixedly installed at the output end of the servo motor. The outer wall of the rotating shaft is fixedly installed inside the center of the centrifuge tank. The bottom end of the rotating shaft is rotatably installed inside the center of the operating table. The rotating shaft passes through the casing. A threaded rod is installed inside the top end of the rotating shaft.
[0009] The pressure plate has a threaded rod fixedly installed at the center of the bottom of the pressure plate. A test tube is sleeved inside the centrifuge barrel. A cup cap is threadedly installed at the top of the test tube. A density sensor is fixedly installed on the inner wall of the bottom of the test tube.
[0010] The test tube has a delivery port fixedly installed at the bottom, and a solenoid valve is provided on the delivery port. A separation cup is threadedly installed at the bottom of the delivery port.
[0011] Preferably, the test tube, cup lid, density sensor, delivery port, solenoid valve, and separation cup are provided in multiple sets, which makes the biological cell separation efficiency higher.
[0012] Preferably, the multiple sets of test tubes, cup lids, density sensors, delivery ports, solenoid valves, and separation cups are arranged in an equally spaced circular array with the center of the circular cross-section of the centrifuge barrel as the array center.
[0013] Preferably, the operating platform is provided with a temperature transfer component, the temperature transfer component includes an air pump, the air pump is fixedly installed on the upper surface of the top of the operating platform, a heater is fixedly installed at the input end of the air pump, and an air inlet is provided at the top of the heater.
[0014] Preferably, one end of a bend is fixedly installed at the output end of the gas pump, and a hollow ring is fixedly installed at the other end of the bend, with a nozzle provided on the hollow ring.
[0015] Preferably, the nozzles are provided in multiple sets, and the multiple sets of nozzles are arranged in a circular array with equal spacing around the center of the circular cross-section of the hollow ring.
[0016] Preferably, an arc-shaped plate is fixedly installed on the arc-shaped outer wall of the centrifuge barrel. Multiple sets of arc-shaped plates are arranged, and the multiple sets of arc-shaped plates are arranged in an equally spaced circular array with the center of the circular cross-section of the centrifuge barrel as the array center, so as to improve the air circulation efficiency inside the casing.
[0017] Compared with the prior art, this utility model provides a centrifuge device for biological cell separation, which has the following beneficial effects:
[0018] 1. This centrifuge for biological cell separation, in order to improve the efficiency of biological cell separation, is equipped with a centrifugation assembly. First, the servo motor is started, which works with the centrifuge bucket, rotating shaft, pressure plate, threaded rod, test tube, and cup lid to separate the biological cells into layers. Then, through the density sensor, delivery port, solenoid valve, and separation cup, the biological cell liquid and the cell material with the higher density after separation are delivered into the separation cup through the delivery port. No manual separation is required, which makes the biological cell separation efficiency higher.
[0019] 2. This centrifuge for biological cell separation incorporates a temperature transfer component to optimize the internal temperature for biological cell separation. When heating is required, the heater is activated, drawing in gas through the air inlet. This, along with the air pump, bend, hollow ring, and nozzle, allows hot gas to enter the casing, increasing the internal temperature. As the centrifuge drum rotates, the arc-shaped plates on its outer wall create turbulence, causing the hot gas to circulate internally. This further enhances the optimal temperature for biological cell separation, resulting in higher separation efficiency. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0021] Figure 2 This is a cross-sectional view of part of the structure of this utility model;
[0022] Figure 3 This is a cross-sectional view of part of the structure of this utility model;
[0023] Figure 4 This is a schematic cross-sectional view of the test tube of this utility model;
[0024] Figure 5 This is a cross-sectional view of the casing of this utility model.
[0025] In the diagram: 1. Control panel; 2. Machine casing; 21. Machine door; 3. Control console; 4. Servo motor; 5. Machine cover; 6. Centrifuge assembly; 61. Centrifuge tank; 62. Rotary shaft; 63. Pressure plate; 64. Threaded rod; 65. Test tube; 66. Cup lid; 67. Density sensor; 68. Delivery port; 69. Solenoid valve; 610. Separation cup; 7. Temperature transfer assembly; 71. Gas pump; 72. Heater; 73. Air inlet; 74. Bend; 75. Hollow ring; 76. Nozzle; 77. Arc plate. Detailed Implementation
[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0027] In this application, the term "above" indicates the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. It is primarily used to better describe this application and its embodiments, and is not intended to limit the indicated device, element, or component to having a specific orientation, or to construct and operate in a specific orientation. Furthermore, the term "above" may also be used in certain circumstances to indicate a dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this application according to the specific circumstances.
[0028] Please see Figure 1 - Figure 5 This utility model provides a technical solution:
[0029] A centrifuge for separating biological cells includes an operating table 1, an organic shell 2 fixedly installed at the center inside the operating table 1, an organic door 21 hinged inside the shell 2, a control console 3 fixedly installed on the top surface of the operating table 1, a servo motor 4 installed on the bottom surface of the operating table 1, an organic cover 5 hinged on the top of the shell 2, and a centrifuge assembly 6 disposed inside the shell 2.
[0030] In one embodiment of this utility model, the centrifuge assembly 6 includes a centrifuge tank 61, which is disposed inside the housing 2. A rotating shaft 62 is fixedly installed at the output end of the servo motor 4. The outer wall of the rotating shaft 62 is fixedly installed inside the center of the centrifuge tank 61. The bottom end of the rotating shaft 62 is rotatably installed inside the center of the operating table 1, and the rotating shaft 62 penetrates the housing 2. A threaded rod 64 is threadedly installed inside the top end of the rotating shaft 62. A pressure plate 63 is used, and the top of the threaded rod 64 is fixedly installed at the bottom center of the pressure plate 63. A test tube 65 is sleeved inside the centrifuge tank 61, and a cup lid 66 is threadedly installed at the top end of the test tube 65. A density sensor 67 is fixedly installed on the inner wall of the bottom end of the test tube 65; a delivery port 68 is fixedly installed on the bottom end of the test tube 65, and a solenoid valve 69 is provided on the delivery port 68. A separation cup 610 is threadedly installed on the bottom end of the delivery port 68. There are multiple sets of test tubes 65, cup lids 66, density sensors 67, delivery ports 68, solenoid valves 69, and separation cups 610. All sets of test tubes 65, cup lids 66, density sensors 67, delivery ports 68, solenoid valves 69, and separation cups 610 are arranged in an equally spaced circular array with the center of the circular cross-section of the centrifuge barrel 61 as the array center.
[0031] In this embodiment, before centrifugation, the operator inserts the test tubes 65 containing the biological cell mixture into the slots of the centrifuge tank 61, covers them with the lid 66, and screws the threaded rod 64 into the rotating shaft 62, causing the pressure plate 63 to press against the top of the lid 66, thus fixing the test tubes 65. The operator starts the servo motor 4 via the control console 3, causing the rotating shaft 62 to rotate and drive the centrifuge tank 61 to rotate. When the centrifuge tank 61 rotates at high speed, the test tubes 65 and their containing biological cell mixture are subjected to centrifugal force. It should be noted that the density sensor 67 is not in operation while the servo motor 4 is working. After the biological cells have completely separated, the servo motor 4 is stopped, and the density sensor 67 is activated via the control console 3 to monitor the density of the biological cell liquid in contact with it in real time. Internally, density thresholds for separating different biological cells are preset, including the density a of the mixed state of biological cells, the density b of the cell sap after stratification, and the density c of the cell material after stratification. When the density sensor 67 detects that the liquid reaches a specific value with a higher preset density (i.e., the density c of the cell material after stratification), the solenoid valve 69 is opened. At this time, the bottom of the test tube 65 is connected to the delivery port 68. The higher-density layered material in the test tube 65 enters the separation cup 610 through the delivery port 68. When the higher-density layered material c is completely discharged from the test tube 65, the density sensor 67 detects that the density of the liquid outside it has decreased, causing the solenoid valve 69 to close, thereby leaving the lower-density layered material b inside the test tube 65, thus completing the biological cell separation work. This reduces the time and effort required for manual separation and significantly improves the separation efficiency of biological cells.
[0032] In one embodiment of this utility model, a temperature transfer component 7 is provided on the operating table 1. The temperature transfer component 7 includes an air pump 71. The air pump 71 is fixedly installed on the upper surface of the top of the operating table 1. A heater 72 is fixedly installed at the input end of the air pump 71. An air inlet 73 is provided at the top of the heater 72. One end of a bent pipe 74 is fixedly installed at the output end of the air pump 71. A hollow ring 75 is fixedly installed at the other end of the bent pipe 74. A nozzle 76 is provided on the hollow ring 75. Multiple sets of nozzles 76 are arranged in a circular array with equal spacing around the center of the circular cross-section of the hollow ring 75. An arc-shaped plate 77 is fixedly installed on the arc-shaped outer wall of the centrifuge barrel 61. Multiple sets of arc-shaped plates 77 are arranged in a circular array with equal spacing around the center of the circular cross-section of the centrifuge barrel 61.
[0033] In this embodiment, when it is necessary to raise the temperature inside the casing 2 to create a suitable environment for biological cell separation, the operator first turns on the heater 72, and outside air enters through the air inlet 73. The incoming air is quickly heated into hot air inside the heater 72. Then, the air pump 71 is started and the hot air is transported through the bend pipe 74. The hot air flows into the hollow ring 75 along the bend pipe 74. After the hot air enters the hollow ring 75, it will be evenly sprayed out through each nozzle 76, thereby evenly distributing the hot air inside the casing 2 and initially raising the overall temperature inside the casing 2. During centrifugation, the centrifuge barrel 61 rotates, causing the arc-shaped plate 77 to rotate as well. The arc-shaped plate 77 generates a turbulent effect on the gas inside the casing 2, causing the hot air sprayed from the nozzle 76 to circulate inside the casing 2, creating a more suitable temperature environment for biological cell separation, thereby improving the efficiency of biological cell separation.
[0034] All electrical components mentioned in this application are electrically connected to the control console 3 and the 220V mains power. The control console 3 is a conventional and known device that can control the servo motor 4, density sensor 67, solenoid valve 69, air pump 71, and other components. All standard parts used in this application can be purchased from the market. The specific connection methods of each part are all conventional methods such as riveting and welding that are mature in the prior art. The machinery, parts, and equipment are all conventional models in the prior art. In addition, the circuit connection adopts conventional connection methods in the prior art, which will not be described in detail here.
[0035] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the protection scope of the present invention.
Claims
1. A centrifuge for separating biological cells, comprising an operating table (1), wherein an organic shell (2) is fixedly installed at the center of the operating table (1), an organic door (21) is hingedly installed inside the organic shell (2), a control console (3) is fixedly installed on the upper surface of the top of the operating table (1), a servo motor (4) is installed on the lower surface of the bottom of the operating table (1), and an organic cover (5) is hingedly installed on the top of the organic shell (2), characterized in that: The inside of the shell (2) is provided with a centrifugal assembly (6), the centrifugal assembly (6) comprises: The inside of the shell (2) is provided with a centrifugal barrel (61), the output end of the servo motor (4) is fixedly installed with a rotating shaft (62), the outer wall of the rotating shaft (62) is fixedly installed in the center of the centrifugal barrel (61), the bottom end of the rotating shaft (62) is rotatably installed in the center of the operation table (1), and the rotating shaft (62) penetrates the shell (2), and the top end of the rotating shaft (62) is internally and threadedly installed with a threaded rod (64); The bottom center of the pressing plate (63) is fixedly installed on the top of the threaded rod (64), the centrifugal barrel (61) is sleeved with a test tube (65), the top end of the test tube (65) is threadedly installed with a cup cover (66), and the inner wall of the bottom end of the test tube (65) is fixedly installed with a density sensor (67); The bottom end of the test tube (65) is fixedly installed with a conveying port (68), the conveying port (68) is provided with a electromagnetic valve (69), and the bottom end of the conveying port (68) is threadedly installed with a separation cup (610).
2. The centrifuge apparatus for separating biological cells according to claim 1, wherein: The test tube (65), the cup cover (66), the density sensor (67), the conveying port (68), the electromagnetic valve (69) and the separation cup (610) are provided with multiple groups.
3. The centrifuge apparatus for separating biological cells according to claim 2, wherein: Multiple groups of the test tube (65), the cup cover (66), the density sensor (67), the conveying port (68), the electromagnetic valve (69) and the separation cup (610) are arranged as an array center with the center of the circular section of the centrifugal barrel (61) as the array center, and are arranged at equal intervals on the circumference.
4. The biological cell separation centrifuge apparatus according to claim 1, wherein: A temperature transmission assembly (7) is arranged on the operation table (1), the temperature transmission assembly (7) comprises a gas conveying pump (71), the top end of the operation table (1) is fixedly installed with the gas conveying pump (71), the input end of the gas conveying pump (71) is fixedly installed with a heater (72), and the top end of the heater (72) is provided with an air inlet (73).
5. The centrifuge apparatus for separating biological cells according to claim 4, wherein: The output end of the gas conveying pump (71) is fixedly installed with one end of a bend pipe (74), the other end of the bend pipe (74) is fixedly installed with a hollow ring (75), and the hollow ring (75) is provided with a nozzle (76).
6. The centrifuge apparatus for separating biological cells according to claim 5, wherein: Multiple groups of the nozzle (76) are arranged, and multiple groups of the nozzle (76) are arranged as an array center with the center of the circular section of the hollow ring (75) as the array center, and are arranged at equal intervals on the circumference.
7. A centrifuge apparatus for separating biological cells according to claim 6, wherein: The arc-shaped outer wall of the centrifugal barrel (61) is fixedly installed with an arc-shaped sheet (77), multiple groups of the arc-shaped sheet (77) are arranged, and multiple groups of the arc-shaped sheet (77) are arranged as an array center with the center of the circular section of the centrifugal barrel (61) as the array center, and are arranged at equal intervals on the circumference.