Intestinal flora detection instrument

Abstract

The utility model discloses an intestinal flora detection instrument which comprises a fluorescent quantitative PCR instrument, a bearing plate is arranged in the fluorescent quantitative PCR instrument in a sliding mode, a baffle plate cover is fixedly connected to the front face of the bearing plate, a bearing seat is arranged at the top of the bearing plate and used for bearing and storing an intestinal flora bearing container, and an installation assembly is arranged at the top of the bearing plate and used for installing the intestinal flora bearing container. The mounting assembly is used for clamping and locking the bearing seat, the mounting shell is arranged on the side of the bearing seat, the locking unit is arranged on the bearing plate, and the locking unit is used for clamping and locking the mounting shell and the bearing seat. The fluorescent quantitative PCR instrument, the bearing plate, the bearing seat and the mounting assembly are used in a matched mode, when the bearing seat and the mounting shell are located on the bearing plate, the locking unit can lock the bearing seat and the mounting shell, and the mounting shell slides away from the bearing seat, so that the mounting shell drives the locking assembly to unlock, and the locking effect is good. Therefore, the bearing seat can be directly detached, cleaned and disinfected.

Description

Technical Field

[0001] This utility model relates to the field of testing instruments, and in particular to an intestinal flora testing instrument. Background Technology

[0002] Gut microbiota testing instruments are devices used to analyze and evaluate the composition and function of the microorganisms in the human gut. These instruments utilize advanced molecular biology techniques and bioinformatics methods to rapidly and accurately detect the diversity, abundance, and function of the gut microbiota, providing important data support for research on gut health, diseases, and personalized medicine.

[0003] The detection of gut microbiota requires sample collection, DNA extraction, PCR amplification, sequencing analysis, data analysis, and result output. Among these steps, PCR amplification involves amplifying the DNA of specific microorganisms through polymerase chain reaction (PCR), and then quantitatively analyzing the abundance of specific microorganisms using a real-time PCR instrument.

[0004] When using a quantitative real-time PCR (qPCR) instrument to detect and analyze gut microbiota, the culture container holding the gut microbiota is usually placed on the qPCR instrument's mounting base. By comparing the sample fluorescence signal with a standard curve, the initial concentration of the target DNA in the sample can be calculated. After each test, the mounting base needs to be disinfected and cleaned to ensure the accuracy of subsequent test data. However, the mounting base of existing qPCR instruments is usually integrated into the sliding mounting plate of the instrument. This makes it inconvenient to disassemble, disinfect, and clean the mounting base after the gut microbiota test is completed, which is a certain drawback. Utility Model Content

[0005] The purpose of this invention is to provide an intestinal flora detection instrument to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an intestinal flora detection instrument, comprising:

[0007] A real-time PCR instrument, wherein a support plate is slidably disposed inside the real-time PCR instrument;

[0008] A baffle cover, which is fixedly connected to the front of the support plate;

[0009] A support base is disposed on top of a support plate and is used to support a container for storing intestinal flora.

[0010] The mounting component is disposed on the top of the support plate and is used to lock the support seat in place.

[0011] Preferably, the mounting components include:

[0012] The mounting housing is disposed on the side of the support base;

[0013] A locking unit is disposed on a support plate and is used to lock the mounting housing and the support base in a snap-fit ​​manner.

[0014] Preferably, the locking unit includes:

[0015] The connecting plate is slidably engaged with the interior of the mounting housing;

[0016] A snap-fit ​​connector, which is fixedly connected to the bottom of the connecting plate;

[0017] A sliding hole is provided on the top of the support plate, and the snap-fit ​​component is slidably snapped into the support plate through the sliding hole.

[0018] Preferably, the locking unit further includes:

[0019] A positioning plate, the top of which is fixedly connected to a bearing seat;

[0020] A connecting hole is provided at the top of the support plate, and the outer wall of the positioning plate is slidably sleeved with the inner cavity of the connecting hole.

[0021] Preferably, the locking unit further includes:

[0022] A locking rod, one end of which is fixedly connected to a snap-fit ​​component;

[0023] A locking hole is provided at the bottom of one side of the positioning plate, and the outer wall of the locking rod is slidably sleeved with the inner cavity of the locking hole.

[0024] Preferably, the locking unit further includes:

[0025] A fixing rod, one end of which is fixedly connected to a bearing seat;

[0026] A slot is formed at the bottom of the mounting housing, and the outer wall of the fixing rod is slidably engaged with the inner cavity of the slot.

[0027] Preferably, the locking unit further includes:

[0028] A through hole is provided on one side of the connecting plate, and the outer wall of the fixing rod is slidably sleeved with the inner cavity of the through hole.

[0029] A positioning rod, one end of which is fixedly connected to a connecting plate;

[0030] The positioning groove is formed on one side of the inner wall of the mounting housing, and the outer wall of the positioning rod is slidably engaged with the inner cavity of the positioning groove.

[0031] Preferably, the locking unit further includes:

[0032] A limiting block, which is fixedly connected to the end of a fixing rod;

[0033] A limiting spring is slidably sleeved on the outside of the fixed rod, and the limiting spring is located between the limiting block and the connecting plate.

[0034] The technical effects and advantages of this utility model are as follows:

[0035] This invention utilizes a combination of a fluorescence quantitative PCR instrument, a carrier plate, a carrier base, and an installation component. The installation component includes an installation housing and a locking unit. When the carrier base and the installation housing are on the carrier plate, the locking unit can lock the carrier base and the installation housing. By sliding the installation housing away from the carrier base, the installation housing can unlock the locking component, thereby allowing the carrier base to be directly disassembled, cleaned, and disinfected. Attached Figure Description

[0036] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0037] Figure 2 This is a schematic diagram of the overall structure of the bearing plate of this utility model;

[0038] Figure 3 This is a schematic diagram of the internal structure of the side of the bearing plate of this utility model;

[0039] Figure 4 This is a bottom view of the internal structure of the mounting housing of this utility model;

[0040] Figure 5 This is a schematic diagram of the overall structure of the snap-fit ​​connector of this utility model.

[0041] In the diagram: 1. Real-time PCR instrument; 2. Support plate; 3. Shielding cover; 4. Support base; 5. Mounting assembly; 51. Mounting housing; 52. Locking unit; 521. Connecting plate; 522. Snap-fit ​​component; 523. Sliding hole; 524. Positioning plate; 525. Locking rod; 526. Fixing rod; 527. Limiting block; 528. Limiting spring; 529. Positioning rod. Detailed Implementation

[0042] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0043] This utility model provides, for example Figure 1-5 The instrument for detecting intestinal flora shown includes a quantitative real-time PCR instrument 1, a shield cover 3, a support base 4, and an installation component 5. A support plate 2 is slidably disposed inside the quantitative real-time PCR instrument 1. The shield cover 3 is fixedly connected to the front of the support plate 2. The support base 4 is disposed on the top of the support plate 2 and is used to support a container for storing intestinal flora. The container for storing intestinal flora is placed on the support base 4, and the shield cover 3 pushes the support plate 2 and the support base 4 to slide into the interior of the quantitative real-time PCR instrument 1. The quantitative real-time PCR instrument 1 generates a fluorescence signal by binding to DNA with its internal fluorescent probes during the PCR reaction. The intensity of the fluorescence signal is proportional to the amount of DNA amplified. The quantitative real-time PCR instrument 1 automatically measures the fluorescence intensity at the end of each cycle and records the data in real time. By comparing the sample fluorescence signal with a standard curve, the initial concentration of the target DNA in the sample can be calculated, thereby detecting intestinal flora. This is existing technology and will not be described in detail here. The installation component 5 is disposed on the top of the support plate 2 and is used to lock the support base 4 in place.

[0044] Furthermore, the mounting assembly 5 includes a mounting housing 51 and a locking unit 52. The mounting housing 51 is located on the side of the support base 4, and the bottom of the mounting housing 51 is open. The locking unit 52 is located on the support plate 2. The locking unit 52 is used to lock the mounting housing 51 and the support base 4. By pulling the mounting housing 51 away from the support base 4, the locking unit 52 can be unlocked. Then, the mounting housing 51 can be used to separate the support base 4 from the support plate 2, thereby disassembling, cleaning and disinfecting the support base 4.

[0045] Furthermore, the locking unit 52 includes a connecting plate 521, a snap-fit ​​member 522, and a sliding hole 523. The connecting plate 521 is slidably snapped into the interior of the mounting housing 51. The snap-fit ​​member 522 is fixedly connected to the bottom of the connecting plate 521. The sliding hole 523 is opened at the top of the support plate 2. The snap-fit ​​member 522 is slidably snapped into the support plate 2 through the sliding hole 523. The snap-fit ​​member 522 is L-shaped, so that the snap-fit ​​member 522 can snap and lock the mounting housing 51.

[0046] In particular, the locking unit 52 also includes a positioning plate 524, a connecting hole, a locking rod 525, and a locking hole. The top of the positioning plate 524 is fixedly connected to the support 4. The connecting hole is opened at the top of the support plate 2. The outer wall of the positioning plate 524 is slidably sleeved with the inner cavity of the connecting hole. One end of the locking rod 525 is fixedly connected to the snap-fit ​​member 522. The locking hole is opened at the bottom of one side of the positioning plate 524. The outer wall of the locking rod 525 is slidably sleeved with the inner cavity of the locking hole. When the snap-fit ​​member 522 snaps into the support 2, the snap-fit ​​member 522 can also lock the positioning plate 524 through the locking rod 525, thereby snapping and locking the support 4 to ensure the stability of the support 4 installed on the support plate 2.

[0047] In particular, the locking unit 52 also includes a fixing rod 526, a slot, a through hole, a positioning rod 529, and a positioning groove. One end of the fixing rod 526 is fixedly connected to the bearing seat 4. The slot is opened at the bottom of the mounting housing 51. The outer wall of the fixing rod 526 is slidably engaged with the inner cavity of the slot. The through hole is opened on one side of the connecting plate 521. The outer wall of the fixing rod 526 is slidably engaged with the inner cavity of the through hole. One end of the positioning rod 529 is fixedly connected to the connecting plate 521. The positioning groove is opened on one side of the inner wall of the mounting housing 51. The outer wall of the positioning rod 529 is slidably engaged with the inner cavity of the positioning groove. Under the action of the positioning rod 529, the connecting plate 521 can be stably engaged inside the mounting housing 51. The connecting plate 521 can also limit the fixing rod 526, thereby ensuring the stability of the fixing rod 526 engaged inside the slot. The connecting plate 521 can also cause the positioning rod 529 to separate from the mounting housing 51, thereby separating the fixing rod 526 from the mounting housing 51 and allowing the mounting housing 51 to be disassembled.

[0048] Specifically, the locking unit 52 also includes a limiting block 527 and a limiting spring 528. The limiting block 527 is fixedly connected to the end of the fixing rod 526, and the limiting spring 528 is slidably sleeved on the outside of the fixing rod 526. The limiting spring 528 is located between the limiting block 527 and the connecting plate 521. The limiting spring 528 can provide a spring force to the connecting plate 521, which can not only make the connecting plate 521 drive the snap-fit ​​part 522 and the locking rod 525 to be in a stable snap-fit ​​state, but also make the connecting plate 521 drive the positioning rod 529 to maintain a snap-fit ​​state with the mounting housing 51.

[0049] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An instrument for detecting intestinal flora, characterized in that, Include: The fluorescence quantitative PCR instrument (1) is internally slidably provided with a bearing plate (2); The shielding plate cover (3) is fixedly connected to the front of the bearing plate (2); The bearing seat (4) is arranged on the top of the bearing plate (2), and the bearing seat (4) is used for bearing the storage intestinal flora bearing container; The mounting assembly (5) is arranged on the top of the bearing plate (2), and the mounting assembly (5) is used for clamping and locking the bearing seat (4).

2. The apparatus for detecting intestinal flora according to claim 1, wherein The mounting assembly (5) comprises: The mounting shell (51) is arranged on the side of the bearing seat (4); The locking unit (52) is arranged on the bearing plate (2), and the locking unit (52) is used for clamping and locking the mounting shell (51) and the bearing seat (4).

3. The intestinal flora detection instrument according to claim 2, characterized in that, The locking unit (52) comprises: The connecting plate (521) is slidably clamped in the inside of the mounting shell (51); The clamping piece (522) is fixedly connected to the bottom of the connecting plate (521); The sliding hole (523) is arranged on the top of the bearing plate (2), and the clamping piece (522) is slidably clamped with the bearing plate (2) through the sliding hole (523).

4. The apparatus for detecting intestinal flora according to claim 3, wherein The locking unit (52) further comprises: The positioning plate (524) is fixedly connected with the bearing seat (4) on the top; The connecting hole is arranged on the top of the bearing plate (2), and the outer wall of the positioning plate (524) is slidably sleeved with the inner cavity of the connecting hole.

5. The apparatus for detecting intestinal flora according to claim 4, wherein The locking unit (52) further comprises: The locking rod (525) is fixedly connected with the clamping piece (522) at one end; The locking hole is arranged on the bottom of one side of the positioning plate (524), and the outer wall of the locking rod (525) is slidably sleeved with the inner cavity of the locking hole.

6. The apparatus for detecting intestinal flora according to claim 5, wherein The locking unit (52) further comprises: The fixed rod (526) is fixedly connected with the bearing seat (4) at one end; The slot is arranged on the bottom of the mounting shell (51), and the outer wall of the fixed rod (526) is slidably clamped with the inner cavity of the slot.

7. The apparatus of claim 6, wherein the apparatus is a fecal microbiome detector. The locking unit (52) further comprises: The through hole is arranged on one side of the connecting plate (521), and the outer wall of the fixed rod (526) is slidably sleeved with the inner cavity of the through hole; The positioning rod (529) is fixedly connected with the connecting plate (521) at one end; The positioning groove is arranged on one side of the inner wall of the mounting shell (51), and the outer wall of the positioning rod (529) is slidably clamped with the inner cavity of the positioning groove.

8. The apparatus for detecting intestinal flora according to claim 7, wherein The locking unit (52) further comprises: The limiting block (527) is fixedly connected to the end of the fixed rod (526); The limiting spring (528) is slidably sleeved on the outside of the fixed rod (526), and the limiting spring (528) is located between the limiting block (527) and the connecting plate (521).

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