Intelligent quantitative cell culture liquid changing device

Abstract

The utility model discloses an intelligent quantitative cell culture liquid changing device, which relates to the field of cell culture and comprises a working table, a placing shell is mounted at the top of the working table, and a plurality of bases are mounted at the lower part in the placing shell. By arranging the plugging assembly, when the extraction pipe or the liquid changing pipe moves downwards to extract liquid or change liquid, the extraction pipe or the liquid changing pipe is firstly in contact with the baffle, and after the baffle is extruded by the extraction pipe or the liquid changing pipe, the baffle moves and drives the sliding block to slide in the sliding groove, the sliding block extrudes the spring, so that the spring is stressed and compressed, and the liquid is extracted or changed. The baffle does not completely shield the top end of the culture bottle any more, the extraction pipe or the liquid changing pipe can move into the culture bottle for liquid extraction or liquid changing, and when the extraction pipe or the liquid changing pipe moves upwards and does not extrude the baffle any more, the spring is stretched to push the sliding block to reset and move, and the baffle is driven to reset and move; after the two baffles are folded, the top end of the culture bottle can be blocked, and external dust or impurities are prevented from entering the culture bottle.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, specifically to an intelligent quantitative cell culture medium exchange device. Background Technology

[0002] Cell culture is a technique that allows cells to grow, multiply, and maintain their main structure and function under artificially created conditions similar to the in vivo environment. Cell culture technology is widely used in biomedical research, drug screening, tissue engineering, gene therapy, and other fields. A cell culture medium replacement device is a device used to automatically replace cell culture medium, aiming to reduce errors and labor intensity of manual operation and improve the efficiency and quality of cell culture.

[0003] According to Chinese Patent No. CN213680738U, a suspension cell combination culture device with a quantitatively controllable structure is disclosed. This utility model can ensure precise control of the drugs added to the suspension cells, while allowing continuous operation of changing the culture flask and adding drugs, thereby improving the speed of changing the culture flask solution and improving work efficiency.

[0004] Regarding the aforementioned patent content, a culture flask, a liquid exchange tube, and an extraction tube are provided to exchange the culture medium inside the culture flask. However, after the liquid exchange, it is difficult to shield the top of the culture flask in time, which may allow external dust or other contaminants to enter the culture flask, thereby contaminating the culture medium and affecting the subsequent culture effect. Utility Model Content

[0005] Based on this, the purpose of this utility model is to provide an intelligent quantitative cell culture medium exchange device to solve the technical problem that external dust may enter the culture bottle and thus contaminate the culture medium.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an intelligent quantitative cell culture medium exchange device, comprising a workbench, a placement shell mounted on the top of the workbench, multiple bases mounted inside the placement shell, and a threaded groove on the top of the base, with a threaded head threadedly connected inside the threaded groove, and a culture flask mounted on the top of the threaded head. A sealing assembly is provided on the upper outer wall of the culture flask, and the sealing assembly includes an installation ring threadedly connected to the upper outer wall of the culture flask. Sliding grooves are provided on both sides of the top of the installation ring, and a spring is installed on one side inside the sliding groove. One end of the spring is connected to a slider, and a baffle is installed on the top of the slider.

[0007] By adopting the above technical solution, when the baffle is squeezed by the extraction tube or the liquid replacement tube, the baffle will move and drive the slider to slide inside the trough, so that the baffle no longer completely covers the top of the culture bottle.

[0008] Furthermore, a fixed frame is installed on the outer surface and back of the workbench. A motor is installed on one side of the fixed frame, and the output end of the motor is connected to a lead screw extending into the fixed frame. A threaded seat is installed on the outer wall of the lead screw.

[0009] By adopting the above technical solution, when it is necessary to move the first support shell or the second support shell, the motor can be started. The operation of the motor can drive the lead screw to rotate, and the lead screw can drive the threaded seat to move, which in turn drives the first support shell or the second support shell to move.

[0010] Furthermore, each of the two threaded seats is equipped with a mounting base, and each of the two mounting bases is equipped with an electric push rod. The output end of one electric push rod is equipped with a first support shell via a piston rod, and the output end of the other electric push rod is equipped with a second support shell via a piston rod.

[0011] By adopting the above technical solution, when it is necessary to adjust the height of the first support shell and the second support shell, the electric push rod can be activated. The operation of the electric push rod can extend or retract the piston rod, thereby driving the first support shell and the second support shell to move.

[0012] Furthermore, a liquid storage tank is installed inside the first support shell, and a liquid level sensor is installed inside the liquid storage tank.

[0013] By adopting the above technical solution, the storage tank can store the culture medium, while the level sensor can monitor the level of the culture medium.

[0014] Furthermore, an inlet pipe extending to the outside of the first support shell is installed on the outer surface of the liquid storage tank, and a sealing plug is fitted at the end of the inlet pipe, and the sealing plug is made of rubber material.

[0015] By adopting the above technical solution, the sealing plug can block the liquid inlet pipe, preventing external dust from entering the liquid storage tank through the liquid inlet pipe.

[0016] Furthermore, a first peristaltic pump and an alarm are respectively installed on the top of the first support shell. The first peristaltic pump is equipped with a first infusion tube, and a liquid guide tube is installed inside the upper part of the first support shell.

[0017] By adopting the above technical solution, the first peristaltic pump can draw culture medium from the storage tank through the first infusion tube, and the culture medium flows into the guide tube through the first infusion tube. The first peristaltic pump has high flow control accuracy, ensuring accurate quantitative measurement for each liquid change.

[0018] Furthermore, the inlet end of the first infusion tube extends into the interior of the storage tank, the outlet end of the first infusion tube extends into the interior of the guide tube, and multiple replacement tubes extending to the outside of the first support shell are installed at the bottom of the guide tube.

[0019] By adopting the above technical solution, when the culture medium enters the liquid guide tube, it can flow into the liquid exchange tube, and then flow into the culture bottle through the liquid exchange tube, thereby achieving the purpose of liquid exchange.

[0020] Furthermore, a collection box is provided at the lower interior of the second support shell, and a liquid outlet pipe extending to the outside of the second support shell is installed on the back of the collection box. A drain valve is installed on the liquid outlet pipe. A drain pipe is installed at the upper interior of the second support shell, and multiple extraction pipes extending to the outside of the second support shell are installed at the bottom of the drain pipe.

[0021] By adopting the above technical solution, when the second peristaltic pump is working, the extraction tube can extract the culture medium inside the culture bottle.

[0022] Furthermore, a second peristaltic pump is installed on the top of the second support shell, and a second infusion tube is provided on the second peristaltic pump. The inlet end of the second infusion tube extends into the inside of the drain tube, and the outlet end of the second infusion tube extends into the inside of the collection box.

[0023] By adopting the above technical solution, the extracted culture medium will flow into the collection box through the second infusion tube, and the collection box can collect the culture medium.

[0024] Furthermore, an AI control panel is installed on one side of the workbench, and the AI ​​control panel is electrically connected to the first peristaltic pump, the second peristaltic pump, the electric push rod, the motor, the alarm, and the liquid level sensor.

[0025] By adopting the above technical solution, the first peristaltic pump, the second peristaltic pump, the electric actuator, the motor, the alarm, and the liquid level sensor can be started or stopped via the AI ​​control panel.

[0026] In summary, the present invention has the following main advantages:

[0027] 1. This utility model, by setting a sealing component, allows the extraction tube or liquid replacement tube to move downwards for liquid extraction or replacement. The extraction tube or liquid replacement tube will first contact the baffle. After being squeezed by the extraction tube or liquid replacement tube, the baffle will move and drive the slider to slide inside the groove. The slider will then squeeze the spring, causing the spring to be compressed. The baffle will no longer completely cover the top of the culture bottle, and the extraction tube or liquid replacement tube can then be moved into the culture bottle for liquid extraction or replacement. When the extraction tube or liquid replacement tube moves upwards and no longer squeezes the baffle, the spring will stretch and push the slider to return to its original position, which will also drive the baffle to return to its original position. After the two baffles close, they can seal the top of the culture bottle, preventing external dust or impurities from entering the culture bottle. In this way, the baffle can promptly cover the top of the culture bottle after the extraction tube or liquid replacement tube is removed, avoiding contamination of the culture medium inside the culture bottle.

[0028] 2. This utility model is equipped with an installation ring. When the baffle needs to be removed, the operator can rotate the installation ring to unscrew it from the culture bottle, thus removing the baffle for easy replacement.

[0029] 3. This utility model has a base, a threaded groove and a threaded head. The culture bottle is installed on the base through the threaded head, which can improve the stability of the culture bottle, prevent the culture bottle from moving, and also make it easy to remove the culture bottle. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention;

[0031] Figure 2 This is a schematic diagram of the overall side structure of this utility model;

[0032] Figure 3 This is a schematic diagram of the orthographic section of the placement shell of this utility model;

[0033] Figure 4 This is a three-dimensional structural diagram of the placement shell of this utility model;

[0034] Figure 5 This is a schematic diagram of the culture bottle structure of this utility model;

[0035] Figure 6 This is a schematic diagram of the mounting base structure of this utility model;

[0036] Figure 7 This is a side sectional view of the first support shell structure of this utility model;

[0037] Figure 8 This is a side sectional view of the second support shell structure of this utility model.

[0038] In the diagram: 1. Workbench; 2. AI control panel; 3. Fixing frame; 4. Lead screw; 5. Threaded seat; 6. Motor; 7. Mounting base; 8. Electric push rod; 9. Sealing assembly; 901. Mounting ring; 902. Spring; 903. Slide groove; 904. Slider; 905. Baffle; 10. Base; 11. First support shell; 12. Liquid guide tube; 13. Liquid replacement tube; 14. Guide rod; 15. Guide hole; 16. 17. Threaded head; 18. First peristaltic pump; 19. First infusion tube; 20. Threaded groove; 21. Storage tank; 22. Liquid level sensor; 23. Inlet pipe; 24. Sealing plug; 25. Second support shell; 26. Drain pipe; 27. Extraction tube; 28. Second peristaltic pump; 29. ​​Second infusion tube; 30. Collection box; 31. Outlet pipe; 32. Placement shell; 33. Culture bottle; 34. Through hole; 35. Alarm. Detailed Implementation

[0039] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0040] The embodiments of this utility model will be described below based on its overall structure.

[0041] Example 1:

[0042] An intelligent quantitative cell culture medium exchange device, such as Figures 1-8 As shown, the device includes a workbench 1, a placement shell 31 mounted on top of the workbench 1, multiple bases 10 mounted inside the lower part of the placement shell 31, and a threaded groove 19 formed on the top of the base 10. A threaded head 16 is threaded into the inside of the threaded groove 19, and a culture flask 32 is mounted on the top of the threaded head 16. Multiple through holes 33 are formed on the top of the placement shell 31, and the culture flask 32 passes through the through holes 33. The upper part of the outer wall of the culture flask 32 is threaded, and the culture flask 32 is detachably connected to the base 10 through the threaded head 16, facilitating the assembly and disassembly of the culture flask 32. The sealing assembly 9 has a fixed frame 3 installed on the outer surface and back of the workbench 1. A motor 6 is installed on one side of the fixed frame 3, and the output end of the motor 6 is connected to a lead screw 4 extending into the fixed frame 3. A threaded seat 5 is installed on the outer wall of the lead screw 4, and the outer wall of the threaded seat 5 fits against the inner wall of the fixed frame 3. One end of the lead screw 4 is connected to the fixed frame 3 through a bearing. When it is necessary to move the first support shell 11 or the second support shell 24, the motor 6 can be started. The operation of the motor 6 can drive the lead screw 4 to rotate, and the lead screw 4 can drive the threaded seat 5 to move, and then drive the first support shell 11 or the second support shell 24 to move.

[0043] See Figures 1-5Each of the two threaded seats 5 is equipped with a mounting base 7. An electric push rod 8 is installed inside each mounting base 7. The output end of one electric push rod 8 is connected to a first support housing 11 via a piston rod, and the output end of the other electric push rod 8 is connected to a second support housing 24 via a piston rod. When the height of the first support housing 11 and the second support housing 24 needs to be adjusted, the electric push rod 8 can be activated. The operation of the electric push rod 8 causes the piston rod to extend or retract, thereby moving the first support housing 11 and the second support housing 24. Guide holes are provided on both sides of the top of each mounting base 7. 15. Two guide rods 14 are installed at the bottom of the first support shell 11 and the bottom of the second support shell 24. The guide rods 14 are located inside the guide hole 15. When the first support shell 11 and the second support shell 24 move, they will drive the guide rods 14 to move within the guide hole 15, so as to improve the stability of the first support shell 11 and the second support shell 24 when moving. A liquid storage tank 20 is installed inside the first support shell 11. A liquid level sensor 21 is installed inside the liquid storage tank 20. The liquid storage tank 20 can store the culture medium, and the liquid level sensor 21 can monitor the liquid level of the culture medium.

[0044] See Figure 1 , Figure 2 , Figure 3 , Figure 6 , Figure 7 and Figure 8 A first peristaltic pump 17 and an alarm 34 are respectively installed on the top of the first support shell 11. The first peristaltic pump 17 is equipped with a first infusion tube 18. A guide tube 12 is installed inside the upper part of the first support shell 11. When the first peristaltic pump 17 works, it can draw culture medium from the inside of the storage tank 20 through the first infusion tube 18. The culture medium flows into the guide tube 12 through the first infusion tube 18. The first peristaltic pump 17 has high flow control accuracy, ensuring accurate quantitative measurement for each culture change. The inlet end of the first infusion tube 18 extends into the inside of the storage tank 20, and the outlet end of the first infusion tube 18 extends into the inside of the guide tube 12. Multiple culture change tubes 13 extending to the outside of the first support shell 11 are installed at the bottom of the guide tube 12. When the culture medium enters the inside of the guide tube 12, it can flow into the culture change tube 13 and then into the culture bottle 32, thereby achieving the purpose of culture change.

[0045] See Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6 , Figure 7 and Figure 8A collection box 29 is located at the lower interior of the second support shell 24, and an outlet pipe 30 extending to the outside of the second support shell 24 is installed on the back of the collection box 29. A drain valve is installed on the outlet pipe 30. A drain pipe 25 is installed at the upper interior of the second support shell 24, and multiple extraction pipes 26 extending to the outside of the second support shell 24 are installed at the bottom of the drain pipe 25. When the second peristaltic pump 27 is working, the extraction pipes 26 can extract the culture medium inside the culture bottle 32. A second peristaltic pump 27 is installed at the top of the second support shell 24, and a second infusion pipe 28 is installed on the second peristaltic pump 27. The inlet end of the second infusion pipe 28 extends into the interior of the drain pipe 25, and the outlet end of the second infusion pipe 28 extends into the interior of the collection box 29. The extracted culture medium flows into the collection box 29 through the second infusion pipe 28. The collection tank 29 can collect the culture medium. An AI control panel 2 is installed on one side of the workbench 1. The AI ​​control panel 2 is electrically connected to the first peristaltic pump 17, the second peristaltic pump 27, the electric push rod 8, the motor 6, the alarm 34, and the liquid level sensor 21. The AI ​​control panel 2 can start or stop the first peristaltic pump 17, the second peristaltic pump 27, the electric push rod 8, the motor 6, the alarm 34, and the liquid level sensor 21. When the liquid level sensor 21 detects that the liquid level of the culture medium inside the storage tank 20 is low, it will transmit a signal to the AI ​​control panel 2. The AI ​​control panel 2 will then activate the alarm 34, which will sound an alarm to remind the surrounding staff that the culture medium inside the storage tank 20 is about to run out, so that the staff can replenish the culture medium in the storage tank 20 later.

[0046] Specifically, the sealing assembly 9 includes a mounting ring 901 threadedly connected to the upper part of the outer wall of the culture flask 32. The mounting ring 901 has grooves 903 on both sides of its top, and a spring 902 is installed on one side of the inner side of the groove 903. One end of the spring 902 is connected to a slider 904. A baffle 905 is installed on the top of the slider 904. One side of the top of the baffle 905 is inclined. One side of every two baffles 905 is in contact with each other. When the baffle 905 is squeezed by the extraction tube 26 or the liquid replacement tube 13, the baffle 905 will move and drive the slider 904 to slide inside the groove 903, so that the baffle 905 no longer completely covers the top of the culture flask 32. One side of the top of the baffle 905 is inclined, and one side of every two baffles 905 is in contact with each other.

[0047] Example 2:

[0048] Based on the above embodiment 1, the following structure will be set up to facilitate the addition of culture medium into the storage tank 20.

[0049] See Figure 1 and Figure 7The outer surface of the liquid storage tank 20 is equipped with an inlet pipe 22 extending to the outside of the first support shell 11. The end of the inlet pipe 22 is fitted with a sealing plug 23, which is made of rubber material. The sealing plug 23 can seal the inlet pipe 22 to prevent external dust from entering the interior of the liquid storage tank 20 through the inlet pipe 22.

[0050] The working principle of this utility model is as follows: First, when in use, the power is turned on. Then, the operator controls the motor 6 corresponding to the second support shell 24 through the AI ​​control panel 2, so that the motor 6 drives the lead screw 4 to rotate, which in turn drives the threaded seat 5 to move. The threaded seat 5 then drives the mounting seat 7 to move, which in turn drives the electric push rod 8 and the second support shell 24 to move. The second support shell 24 drives the extraction tube 26 to move. When the extraction tube 26 moves directly above the culture bottle 32, the motor 6 is turned off, and then the electric push rod 8 is started, which causes the piston rod to retract, thereby driving the second support shell 24 to move down, and then driving the extraction tube 26 to move down. The extraction tube 26 will first contact the baffle. After the baffle 905 is squeezed by the extraction tube 26, the baffle 905 will move and drive the slider 904 to slide. The slide inside the groove 903 causes the slider 904 to compress the spring 902, thus compressing the spring 902. The baffle 905 no longer completely covers the top of the culture flask 32, allowing the extraction tube 26 to be inserted into the culture flask 32. Then, the second peristaltic pump 27 is activated, causing the extraction tube 26 to extract the culture medium from the culture flask and discharge it into the collection tank 29. After extraction, the electric push rod 8 extends the piston rod, causing the second support shell 24 to move upwards, which in turn moves the extraction tube 26 upwards, allowing it to exit the culture flask 32. At this point, the baffle 905 is no longer compressed by the extraction tube 26, and the spring 902 stretches, pushing the slider 904 to reset and move, which in turn moves the baffle 905 to reset and block the culture flask 32.

[0051] Next, the motor 6 corresponding to the first support shell 11 is controlled by the AI ​​control panel 2 to move the first support shell 11 laterally and move it above the culture bottle 32. Then, the corresponding electric push rod 8 is activated to move the first support shell 11 downward, which in turn moves the liquid replacement tube 13 downward. When the liquid replacement tube 13 moves downward, it also pushes the baffle 905 to move, so that the baffle 905 no longer blocks the culture bottle 32. After the liquid replacement tube 13 moves into the culture bottle 32, the first peristaltic pump 17 is activated. The first peristaltic pump 17 can draw the culture medium inside the storage tank 20 through the first infusion tube 18, so that the culture medium flows into the culture bottle 32 through the liquid replacement tube 13, thereby completing the liquid replacement work. The first peristaltic pump 17 is set to replace the liquid quantitatively.

[0052] After the medium replacement is completed, the first support shell 11 is moved upward and reset, and then the medium replacement tube 13 is moved upward so that it is removed from the culture bottle 32. At this time, the baffle 905 is no longer squeezed by the medium replacement tube 13. The spring 902 is stretched and pushes the slider 904 to reset and move, and then drives the baffle 905 to reset and move, so that the baffle 905 blocks the culture bottle 32 to prevent external dust from entering the culture bottle 32 and contaminating the culture medium.

[0053] The liquid level sensor 21 can detect the liquid level of the culture medium inside the storage tank 20. When the liquid level is low, it will transmit a signal to the AI ​​control panel 2, which will then activate the alarm 34. The alarm 34 will sound an alarm to remind the surrounding staff that the culture medium in the storage tank 20 is about to run out. When it is necessary to add culture medium into the storage tank 20, the staff will first pull the sealing plug 23, and then add the culture medium into the storage tank 20 through the inlet pipe 22. After adding the culture medium, the sealing plug 23 will be inserted into the inlet pipe 22 to seal it.

[0054] When it is necessary to remove the culture bottle 32, the culture bottle 32 can be rotated so that the threaded head 16 is unscrewed from the threaded groove 19, and then the culture bottle 32 can be removed.

[0055] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. An intelligent quantitative cell culture medium replacement device, comprising a workbench (1), characterized in that: The top of the workbench (1) is provided with a placing shell (31), a plurality of bases (10) are installed below the inside of the placing shell (31), a threaded groove (19) is formed in the top of the base (10), a threaded head (16) is threadedly connected in the inside of the threaded groove (19), a culture bottle (32) is installed on the top of the threaded head (16), a blocking assembly (9) is arranged above the outer wall of the culture bottle (32), and the blocking assembly (9) comprises an installation ring (901) threadedly connected above the outer wall of the culture bottle (32), a sliding groove (903) is formed in the top of each side of the installation ring (901), a spring (902) is installed in one side of the inside of the sliding groove (903), one end of the spring (902) is connected with a sliding block (904), and a baffle (905) is installed on the top of the sliding block (904).

2. The intelligent quantitative cell culture medium changing device according to claim 1, characterized in that: The outer surface and the back of the workbench (1) are provided with a fixed frame (3), one side of the fixed frame (3) is provided with a motor (6), the output end of the motor (6) is connected with a lead screw (4) extending into the inside of the fixed frame (3), and the outer wall of the lead screw (4) is provided with a threaded seat (5).

3. The intelligent quantitative cell culture medium changing device according to claim 2, characterized in that: Two installation seats (7) are installed on the threaded seats (5), and an electric push rod (8) is installed in the inside of each installation seat (7), the output end of one electric push rod (8) is provided with a first supporting shell (11) through a piston rod, and the output end of the other electric push rod (8) is provided with a second supporting shell (24) through a piston rod.

4. The intelligent quantitative cell culture medium changing device according to claim 3, characterized in that: The inside of the first supporting shell (11) is provided with a liquid storage tank (20), and the inside of the liquid storage tank (20) is provided with a liquid level sensor (21).

5. The intelligent quantitative cell culture medium changing device according to claim 4, characterized in that: The outer surface of the liquid storage tank (20) is provided with a liquid inlet pipe (22) extending to the outside of the first supporting shell (11), the end of the liquid inlet pipe (22) is provided with a blocking plug (23), and the blocking plug (23) is made of rubber material.

6. The intelligent quantitative cell culture medium changing device according to claim 3, characterized in that: The top of the first supporting shell (11) is provided with a first peristaltic pump (17) and an alarm (34), respectively, the first peristaltic pump (17) is provided with a first infusion tube (18), and the inside of the first supporting shell (11) is provided with a liquid guide pipe (12) above.

7. The intelligent quantitative cell culture medium changing device according to claim 6, characterized in that: The liquid inlet end of the first infusion tube (18) extends to the inside of the liquid storage tank (20), the liquid outlet end of the first infusion tube (18) extends to the inside of the liquid guide pipe (12), and the bottom of the liquid guide pipe (12) is provided with a plurality of liquid exchange pipes (13) extending to the outside of the first supporting shell (11).

8. The intelligent quantitative cell culture medium changing device according to claim 3, characterized in that: The inside of the second supporting shell (24) is provided with a collection tank (29) below, the back of the collection tank (29) is provided with a liquid outlet pipe (30) extending to the outside of the second supporting shell (24), the liquid outlet pipe (30) is provided with a liquid discharge valve, the inside of the second supporting shell (24) is provided with a liquid discharge pipe (25) above, and the bottom of the liquid discharge pipe (25) is provided with a plurality of extraction pipes (26) extending to the outside of the second supporting shell (24).

9. The intelligent quantitative cell culture medium changing device according to claim 8, characterized in that: The top of the second support shell (24) is provided with a second peristaltic pump (27), and the second peristaltic pump (27) is provided with a second infusion tube (28), the liquid inlet end of the second infusion tube (28) extends to the inside of the drainage tube (25), and the liquid outlet end of the second infusion tube (28) extends to the inside of the collecting box (29).

10. The intelligent quantitative cell culture medium changing device according to claim 1, characterized in that: One side of the workbench (1) is provided with an AI control panel (2), and the AI control panel (2) is electrically connected with the first peristaltic pump (17), the second peristaltic pump (27), the electric push rod (8), the motor (6), the alarm (34) and the liquid level sensor (21) respectively.

Images