DC-CIK cell culture set

Abstract

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of DC-CIK cell culture biotechnology, and in particular to a DC-CIK cell culture kit. Background Technology

[0002] DC cells have been extensively studied for their use in tumor immunotherapy, where they activate specific T cells by loading tumor antigens.

[0003] DC-CIK cell culture combines DC cells with CIK cells. The antigen-presenting function of DC cells activates specific immunity, while the broad-spectrum killing ability of CIK cells creates a synergistic anti-tumor effect. Multiple clinical trials have validated the safety and preliminary efficacy of DC-CIK cells in solid tumors such as lung cancer and liver cancer, promoting their clinical application.

[0004] In actual production, existing DC-CIK cell culture methods require frequent cell transfers, which greatly increases the risk of cell culture and places high demands on the technical skills of producers. Utility Model Content

[0005] The purpose of this invention is to provide a technical solution for DC-CIK cell culture kit to address the shortcomings of existing technologies. This kit not only enables the entire process of DC-CIK cell culture without cell transfer, but also reduces the risks in the cell culture process, lowers the technical requirements for producers, and improves the efficiency of cell culture.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0007] DC-CIK cell culture kit, characterized in that: it includes...

[0008] At least one DC flask for culturing DC cells;

[0009] At least one CIK flask, which is detachably connected to the DC flask, for culturing CIK cells;

[0010] At least one DC-CIK culture flask, which can be detachably connected to a DC flask or a CIK flask, for culturing DC-CIK cells;

[0011] And at least one culture bag, which is detachably connected to the DC-CIK culture flask via a hose with an opening and closing mechanism.

[0012] The above-mentioned structural design not only enables the entire process of DC-CIK cell culture without cell transfer, but also reduces the risks in the cell culture process, lowers the technical requirements for producers, and improves the efficiency of cell culture.

[0013] Furthermore, both the DC bottle and the CIK bottle include a bottle body and T-shaped blocks and buckles on both sides of the bottle body. The buckles are provided with T-shaped grooves that match the T-shaped blocks. By embedding the T-shaped blocks into the T-shaped grooves, the DC bottle and the CIK bottle can be connected. By splicing and connecting the DC bottle and the CIK bottle, the mixing of DC cells and CIK cells can be achieved without opening the sealed end caps, which greatly improves the efficiency of cell culture and greatly reduces the risk of metastasis.

[0014] Furthermore, the T-block is provided with a mating ring, and the inner side of the mating ring is provided with a threaded hole that extends horizontally into the interior of the bottle. The buckle plate is provided with a through hole that extends horizontally into the interior of the bottle. The through hole matches the mating ring. When the DC bottle and CIK bottle are connected, the mating ring on the unconnected T-block is sealed by the sealing side cover, and the through hole on the unconnected buckle plate is sealed by the sealing plate. The sealing plate is also provided with a second sealing groove, which is conducive to the installation of the sealing ring.

[0015] Furthermore, the T-shaped block is provided with a first sealing groove, and the T-shaped groove is provided with a second sealing groove. The first sealing groove and the second sealing groove match each other. When the T-shaped block is embedded in the T-shaped groove, a sealing ring is set between the first sealing groove and the second sealing groove, which can improve the sealing effect when the DC bottle and CIK bottle are connected and prevent cells from flowing out from the connection.

[0016] Furthermore, the top and bottom of the bottle are respectively equipped with an inlet pipe and an outlet pipe, both of which are provided with external threads. The inlet pipe is threaded with a sealing end cap, and the outlet pipe is threaded with a sealing end cap or threaded with a DC-CIK culture bottle. The inlet and outlet pipes facilitate the placement of cells into the bottle or the flow of cultured cells into the DC-CIK culture bottle, and the sealing end caps serve a sealing function.

[0017] Furthermore, the opening and closing mechanism includes a first opening and closing cover, a second opening and closing cover, and an opening and closing assembly. The second opening and closing cover is connected to the first opening and closing cover through the opening and closing assembly. When the first opening and closing cover and the second opening and closing cover rotate relative to each other, the opening and closing assembly opens or closes, thereby enabling the hose to be connected or closed.

[0018] Furthermore, the opening and closing assembly includes an opening and closing block, a push-pull rod, a rotating ring, and a guide rod. The opening and closing blocks are distributed in a ring within the first annular groove of the first opening and closing cover and are rotatably connected to the first opening and closing cover. The rotating ring is rotatably connected within the first annular groove. Ear plates are evenly distributed on the inner side of the rotating ring and are connected to the opening and closing blocks via the push-pull rod. The side of the first opening and closing cover has at least two guide grooves. The guide rod is located on the outer side of the rotating ring, passes through the guide groove, and moves along the guide groove. The rotating ring drives the ear plates to move in a circumferential direction, and the push-pull rod can then drive the opening and closing blocks to rotate, thereby realizing the opening and closing or closing of adjacent opening and closing blocks. This is beneficial for controlling the opening and closing of the hose. The guide rod moves along the guide groove and can limit the rotational position between the first opening and closing cover and the second opening and closing cover.

[0019] Furthermore, the top surface of the rotating ring is uniformly provided with fixing rods, which are connected to the second annular groove of the second opening and closing cover. The fixing rods improve the connection stability between the second opening and closing cover and the rotating ring, which is beneficial for driving the rotating ring to rotate through the second opening and closing cover.

[0020] Furthermore, the top surface of the first opening and closing cover is provided with two positioning holes, the distance between the two positioning holes is matched with the size of the guide groove, and the bottom surface of the second opening and closing cover is provided with an elastic block, which is matched with the positioning hole. The design of the elastic block and the positioning hole is conducive to positioning the first opening and closing cover and the second opening and closing cover.

[0021] Furthermore, the first opening and closing cover is provided with a first through hole, which connects to the first annular groove, and the second opening and closing cover is provided with a second through hole, which connects to the second annular groove.

[0022] This utility model, by adopting the above-mentioned technical solution, has the following beneficial effects:

[0023] 1. This invention not only enables the completion of the entire process of DC-CIK cell culture without cell transfer, but also reduces the risks in the cell culture process, while reducing the technical requirements for producers and improving the efficiency of cell culture.

[0024] 2. By connecting DC and CIK bottles, DC cells and CIK cells can be mixed without opening the sealed end caps, which greatly improves the efficiency of cell culture and significantly reduces the risk of metastasis.

[0025] 3. The rotating ring drives the ear plate to move in a circumferential direction, which in turn drives the opening and closing block to rotate through the push-pull rod, realizing the opening and closing or closing between adjacent opening and closing blocks. This is beneficial for controlling the opening and closing of the hose. The guide rod moves along the guide groove, which can limit the rotation position between the first opening and closing cover and the second opening and closing cover. Attached image description:

[0026] The present invention will be further described below with reference to the accompanying drawings:

[0027] Figure 1 This is a rendering of the DC-CIK cell culture kit of this utility model;

[0028] Figure 2 for Figure 1 Schematic diagram of the structure in direction A;

[0029] Figure 3 This is a schematic diagram of the structure of the CIK bottle or DC bottle in this utility model;

[0030] Figure 4 This is a schematic diagram of the structure of the CIK bottle or DC bottle after removing the sealing end cap on the discharge pipe in this utility model;

[0031] Figure 5 This is a schematic diagram of the opening and closing mechanism in this utility model;

[0032] Figure 6 This is a schematic diagram showing the connection between the opening and closing component and the first opening and closing cover in this utility model;

[0033] Figure 7 for Figure 6 A schematic diagram of the structure in the B direction.

[0034] In the diagram: 1-DC bottle; 101-bottle body; 102-T-block; 103-clasp plate; 104-fitting ring; 105-threaded hole; 106-T-slot; 107-first sealing groove; 108-feed pipe; 109-sealing end cap; 110-second sealing groove; 111-discharge pipe; 112-through hole;

[0035] 2-DC-CIK culture flask; 3-culture bag; 4-tube;

[0036] 5- Opening and closing mechanism;

[0037] 51-First opening / closing cover; 5101-Guide groove; 5102-First annular groove; 5103-First through hole;

[0038] 52-Second opening and closing cover; 5201-Second through hole; 5202-Second annular groove; 5203-Elastic block;

[0039] 53-Opening and closing assembly; 5301-Guide rod; 5302-Opening and closing block; 5303-Push-pull rod; 5304-Rotating ring; 5305-Ear plate; 5306-Fixing rod; 5307-Positioning hole;

[0040] 6-Sealed side cap; 7-Sealed plate; 8-CIK bottle. Detailed Implementation

[0041] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0042] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of the present invention.

[0043] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this utility model are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion.

[0044] like Figures 1 to 7 The image shows the DC-CIK cell culture kit of this invention, comprising at least one DC bottle 1, at least one CIK bottle 8, at least one DC-CIK culture flask 2, and at least one culture bag 3. This application uses one DC bottle 1, one CIK bottle 8, one DC-CIK culture flask 2, and one culture bag 3 as an example for illustration.

[0045] DC bottle 1 is used to culture DC cells; CIK bottle 8 is detachably connected to DC bottle 1 and used to culture CIK cells.

[0046] Both DC bottle 1 and CIK bottle 8 include a bottle body 101 and T-shaped blocks 102 and buckle plates 103 located on both sides of the bottle body 101. The buckle plate 103 is provided with a T-shaped groove 106, which matches the T-shaped block 102. By embedding the T-shaped block 102 into the T-shaped groove 106, the DC bottle 1 and CIK bottle 8 are connected. By splicing and connecting DC bottle 1 and CIK bottle 8, DC cells and CIK cells can be mixed without opening the sealed end cap 109, which greatly improves the efficiency of cell culture and greatly reduces the risk of metastasis.

[0047] The T-block 102 is provided with a mating ring 104, and the inner side of the mating ring 104 is provided with a threaded hole 105. The threaded hole 105 extends horizontally into the interior of the bottle body 101. The buckle plate 103 is provided with a through hole 112, which extends horizontally into the interior of the bottle body 101. The through hole 112 matches the mating ring 104. When the DC bottle 1 and the CIK bottle 8 are connected, the mating ring 104 on the unconnected T-block 102 is sealed by the sealing side cover 6, and the through hole 112 on the unconnected buckle plate 103 is sealed by the sealing plate 7. The sealing plate 7 is also provided with a second sealing groove 110, which is conducive to the installation of the sealing ring.

[0048] The T-shaped block 102 is provided with a first sealing groove 107, and the T-shaped groove 106 is provided with a second sealing groove 110. The first sealing groove 107 and the second sealing groove 110 are matched. When the T-shaped block 102 is embedded in the T-shaped groove 106, a sealing ring is set between the first sealing groove 107 and the second sealing groove 110, which can improve the sealing effect when the DC bottle 1 and the CIK bottle 8 are connected, and prevent cells from flowing out from the connection.

[0049] The top and bottom of the bottle body 101 are respectively provided with an inlet pipe 108 and an outlet pipe 111. Both the inlet pipe 108 and the outlet pipe 111 are provided with external threads. The inlet pipe 108 is threaded with a sealing end cap 109, and the outlet pipe 111 is threaded with a sealing end cap 109 or threaded with a DC-CIK culture bottle 2. The inlet pipe 108 and the outlet pipe 111 facilitate the placement of cells into the bottle body 101 or the flow of cultured cells into the DC-CIK culture bottle 2. The sealing end cap 109 serves a sealing function.

[0050] DC-CIK culture flask 2 can be detachably connected to DC flask 1 or CIK flask 8 for culturing DC-CIK cells; culture bag 3 can be detachably connected to DC-CIK culture flask 2 via tubing 4, and tubing 4 is equipped with an opening and closing mechanism 5.

[0051] The opening and closing mechanism 5 includes a first opening and closing cover 51, a second opening and closing cover 52, and an opening and closing component 53. The second opening and closing cover 52 is connected to the first opening and closing cover 51 through the opening and closing component 53. When the first opening and closing cover 51 and the second opening and closing cover 52 rotate relative to each other, the opening and closing component 53 opens or closes, thereby enabling the hose 4 to be connected or closed.

[0052] The opening and closing assembly 53 includes an opening and closing block 5302, a push-pull rod 5303, a rotating ring 5304, and a guide rod 5301. The opening and closing block 5302 is arranged in a ring within the first annular groove 5102 of the first opening and closing cover 51. The opening and closing block 5302 is rotatably connected to the first opening and closing cover 51. The rotating ring 5304 is rotatably connected within the first annular groove 5102. Ear plates 5305 are evenly provided on the inner side of the rotating ring 5304. The ear plates 5305 are connected to the opening and closing block 5302 via the push-pull rod 5303. The side of the first opening and closing cover 51 is provided with at least two guide grooves 510. 1. The guide rod 5301 is located on the outer surface of the rotating ring 5304. The guide rod 5301 passes through the guide groove 5101 and moves along the guide groove 5101. The rotating ring 5304 drives the ear plate 5305 to move in the circumferential direction. In turn, the push-pull rod 5303 drives the opening and closing block 5302 to rotate, realizing the opening and closing or closing between adjacent opening and closing blocks 5302. This is beneficial for controlling the switch of the hose 4. The guide rod 5301 moves along the guide groove 5101, which can limit the rotation position between the first opening and closing cover 51 and the second opening and closing cover 52.

[0053] The top surface of the rotating ring 5304 is uniformly provided with fixing rods 5306. The fixing rods 5306 are connected to the second annular groove 5202 of the second opening and closing cover 52. The fixing rods 5306 improve the connection stability between the second opening and closing cover 52 and the rotating ring 5304, which is conducive to driving the rotating ring 5304 to rotate through the second opening and closing cover 52.

[0054] The top surface of the first opening cover 51 is provided with two positioning holes 5307, and the distance between the two positioning holes 5307 matches the size of the guide groove 5101. The bottom surface of the second opening cover 52 is provided with an elastic block 5203, which matches the positioning holes 5307. The design of the elastic block 5203 and the positioning holes 5307 facilitates the positioning of the first opening cover 51 and the second opening cover 52.

[0055] The first opening and closing cover 51 is provided with a first through hole 5103, which connects to the first annular groove 5102. The second opening and closing cover 52 is provided with a second through hole 5201, which connects to the second annular groove 5202.

[0056] The above-mentioned structural design not only enables the entire process of DC-CIK cell culture without cell transfer, but also reduces the risks in the cell culture process, lowers the technical requirements for producers, and improves the efficiency of cell culture.

[0057] In practical use, this invention first selects a DC bottle and a CIK bottle, splices them together, and installs a sealing strip. A sealing side cover is installed on the mating ring of the DC bottle, and a sealing plate is installed on the through-hole of the CIK bottle for sealing. Next, DC cells and CIK cells are added to the DC bottle and CIK bottle respectively for culture. After the required culture time, the DC cells and CIK cells are mixed and introduced into a DC-CIK culture bottle for DC-CIK cell culture. After culture, the opening and closing mechanism is opened, allowing the DC-CIK cells in the DC-CIK culture bottle to flow into a culture bag for storage.

[0058] The above are merely specific embodiments of this utility model, but the technical features of this utility model are not limited thereto. Any simple changes, equivalent substitutions, or modifications made based on this utility model to achieve essentially the same technical effect are all covered within the protection scope of this utility model.

Claims

1. A DC-CIK cell culture kit, characterized in that: The utility model provides a DC-CIK culture device, which comprises at least one DC bottle for culturing DC cells, at least one CIK bottle detachably connected to the DC bottle for culturing CIK cells, at least one DC-CIK culture bottle detachably connected to the DC bottle or the CIK bottle for culturing DC-CIK cells, and at least one culture bag detachably connected to the DC-CIK culture bottle through a hose provided with an opening and closing mechanism. The DC bottle and the CIK bottle each comprise a bottle body and T-shaped blocks and buckling plates arranged on both sides of the bottle body, the buckling plates are provided with T-shaped grooves matched with the T-shaped blocks, the T-shaped blocks are embedded in the T-shaped grooves to realize the matching connection between the DC bottle and the CIK bottle. The T-shaped blocks are provided with matching rings, the inner side of the matching rings is provided with threaded holes extending to the inside of the bottle body in the horizontal direction, the buckling plates are provided with through holes extending to the inside of the bottle body in the horizontal direction, and the through holes are matched with the matching rings. The T-shaped blocks are provided with first sealing grooves, and the T-shaped grooves are provided with second sealing grooves matched with the first sealing grooves, and a sealing ring is arranged between the first sealing grooves and the second sealing grooves when the T-shaped blocks are embedded in the T-shaped grooves. 2.The DC-CIK cell culture kit of claim 1, wherein: The top and bottom of the bottle body are respectively provided with a feeding pipe and a discharging pipe, the feeding pipe and the discharging pipe are each provided with external threads, the feeding pipe is threadedly connected with a sealing end cover, and the discharging pipe is threadedly connected with the sealing end cover or threadedly connected with the DC-CIK culture bottle. 3.The DC-CIK cell culture kit of claim 2, wherein: The opening and closing mechanism comprises a first opening and closing cover, a second opening and closing cover, and an opening and closing assembly, the second opening and closing cover is connected to the first opening and closing cover through the opening and closing assembly, the opening and closing assembly is opened or closed when the first opening and closing cover is rotated relative to the second opening and closing cover, and the hose is opened or closed. 4.The DC-CIK cell culture kit of claim 2, wherein: The opening and closing assembly comprises an opening and closing block, a push-pull rod, a rotating ring, and a guide rod, the opening and closing block is annularly arranged in a first annular groove of the first opening and closing cover, the opening and closing block is rotationally connected to the first opening and closing cover, the rotating ring is rotationally connected to the first annular groove, the inner side surface of the rotating ring is uniformly provided with ear plates, the ear plates are connected to the opening and closing block through the push-pull rod, the side surface of the first opening and closing cover is provided with at least two guide grooves, the guide rod is arranged on the outer side surface of the rotating ring, the guide rod penetrates through the guide grooves and moves along the guide grooves.

5. The DC-CIK cell culture kit according to claim 2, wherein: The top surface of the rotating ring is uniformly provided with fixing rods connected to a second annular groove of the second opening and closing cover. 6.The DC-CIK cell culture kit of claim 1, wherein: The top surface of the first opening and closing cover is provided with two positioning holes, the distance between the two positioning holes is matched with the size of the guide grooves, the bottom surface of the second opening and closing cover is provided with elastic blocks matched with the positioning holes.

7. The DC-CIK cell culture kit according to claim 6, wherein: The first opening and closing cover is provided with a first through hole, and the first through hole is connected with the first annular groove; the second opening and closing cover is provided with a second through hole, and the second through hole is connected with the second annular groove.

8. The DC-CIK cell culture kit according to claim 7, characterized in that: ​ 9. The DC-CIK cell culture kit according to claim 7, characterized in that: ​ 10. The DC-CIK cell culture kit according to claim 8, characterized in that: ​

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