Shaking table for suspension cell culture

Abstract

The utility model provides a shaking table for culturing suspension cells, and belongs to the technical field of cell culture shaking tables. Comprising a shaking table body, a culture chamber is arranged in the shaking table body, a tray for placing shake flasks is arranged in the culture chamber, a limiting frame is arranged above the tray in parallel, a plurality of elastic belts are detachably arranged on the limiting frame, and a plurality of limiting holes for shake flask necks to pass through are formed in the limiting frame through the elastic belts. One side edge of the limiting frame is hinged to the tray, and the other side edge is detachably connected with the tray clamp. The shake flasks are placed on the tray, then the limiting frame is rotated until the shake flasks are flatly placed on the supporting frame, the necks of the shake flasks penetrate through the limiting holes formed by the elastic belts in the limiting frame, and through limitation of the elastic belts, the phenomena that the shake flasks are placed unstably and collide with one another due to the fact that the shake flasks move under the inertia acting force when the tray is suddenly stopped can be effectively avoided; the effect of improving the placing stability of the shake flask on the tray is achieved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture shaker technology, and in particular to a shaker for suspension cell culture. Background Technology

[0002] Laboratory shakers (also known as oscillators) are commonly used equipment in biological and chemical laboratories. They achieve the mixing, dissolution, cultivation, or homogenization of samples through controlled vibration or rotation. Suspension cell culture refers to the growth of cells in a free state in a culture medium. To simulate a dynamic culture environment and solve key problems that cannot be met in static culture, such as oxygen transfer, nutrient uniformity, and cell suspension stability, suspension cells are usually contained in shake flasks and placed in a shaker for cultivation.

[0003] Currently, during operation, the frequent reciprocating motion of the shaker reduces the inertial force on the flasks at the end of the shaker's travel, ensuring that the flasks and other containers remain stable on the tray. However, some equipment has an emergency stop function when the operator opens the shaker cabinet door to retrieve or place flasks. When the tray is at the travel end, this emergency stop can easily cause the flasks on the tray to become unstable, and when multiple flasks are stored simultaneously, they are more likely to collide with each other, potentially leading to breakage. Utility Model Content

[0004] In view of the above problems, this utility model provides a shaker for suspension cell culture.

[0005] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:

[0006] A shaker for suspension cell culture is provided, comprising a shaker body, a culture chamber inside the shaker body, a tray for placing shake flasks inside the culture chamber, a drive component for driving the tray to reciprocate, a limit frame parallel to the top of the tray, a plurality of elastic bands detachably provided on the limit frame, and a plurality of limit holes for the shaker bottle to pass through formed by the multiple elastic bands on the limit frame, one edge of the limit frame being hinged to the tray, and the other edge being detachably connected to the tray.

[0007] Furthermore, multiple limiting pins are fixedly provided along the circumference of the edge of the limiting frame, and connecting buckles are fixedly provided at both ends of the elastic band, with the connecting buckles movably engaging with two adjacent limiting pins.

[0008] Furthermore, each limiting pin can accommodate at least two connecting buckles vertically.

[0009] Furthermore, support frames are vertically fixed on both sides of the tray, with one end of the limiting frame hinged to the support frame and the other end magnetically connected to the support frame.

[0010] Furthermore, the tray is equipped with several anti-slip parts made of rubber.

[0011] Furthermore, multiple anti-slip parts are arranged in a rectangular array on the tray, and a limiting groove for inserting the bottom of the test tube is formed between four adjacent anti-slip parts arranged in a rectangular array.

[0012] Furthermore, the drive assembly includes a transmission frame and a drive motor. At least three turntables are rotatably mounted on the inner bottom wall of the culture chamber. Each turntable has an eccentrically mounted connecting column on its top wall. The transmission frame is fixedly mounted on the bottom wall of the tray. The connecting column is rotatably connected to the transmission frame. The drive motor is used to drive any one of the turntables to rotate.

[0013] The beneficial effects of this utility model are as follows: When placing the culture flask of suspended cells into a shaker for shaking culture, the flask is placed on a tray, and then the limiting frame is rotated to be placed flat on the support frame, so that the neck of the flask passes through the limiting hole made of elastic band on the limiting frame. By limiting it with elastic band, the phenomenon of the flask moving under inertial force when the tray stops suddenly can be effectively avoided, which would cause the flask to be unstable and to collide between multiple flasks, thus protecting the flask. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the shaker body according to an embodiment of this application.

[0015] Figure 2 This is a schematic diagram of the exploded structure inside the shaker according to an embodiment of this application.

[0016] The components include: 1. Shaker body; 11. Culture chamber; 2. Tray; 21. Support frame; 22. Anti-slip part; 23. Limiting groove; 3. Limiting frame; 31. Limiting pin; 4. Elastic band; 41. Limiting hole; 42. Connecting buckle; 51. Transmission frame; 52. Drive motor; 53. Turntable; 54. Connecting column. Detailed Implementation

[0017] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0018] This application discloses a shaker for suspension cell culture, referring to... Figure 1 and Figure 2 The device includes a shaker body 1, which contains a culture chamber 11. A cabinet door for opening and closing the culture chamber 11 is rotatably mounted on the shaker body 1. A tray 2 for placing shake flasks is provided inside the culture chamber 11, and a drive assembly for driving the tray 2 to reciprocate is provided inside the shaker body 1.

[0019] In this embodiment, the drive assembly can consist of a transmission frame 51 and a drive motor 52. At least three turntables 53 are rotatably mounted on the inner bottom wall of the culture chamber 11. Each turntable 53 has an eccentrically mounted connecting post 54 on its top wall. The transmission frame 51 is fixedly mounted on the bottom wall of the tray 2. The connecting post 54 on each turntable 53 is rotatably connected to the transmission frame 51 via bearings. When the three connecting posts 54 are connected to the transmission frame 51, the connecting posts 54 on the three turntables 53 are all eccentrically located at the same angular position. The drive motor 52 is fixedly mounted inside the shaker body. The output shaft of the drive motor 52 is rotatably connected to any one of the turntables 53 to drive the turntable 53 to rotate.

[0020] A limiting frame 3 is installed parallel to the tray 2 above the culture chamber 11. Multiple elastic bands 4 are detachably installed on the limiting frame 3. The multiple elastic bands 4 are arranged in a crisscross pattern on the limiting frame 3 to form several limiting holes 41 for the neck of the shake flask to pass through. One edge of the limiting frame 3 is hinged to the tray 2, and the other edge is detachably connected to the tray 2. After the shake flask is placed on the tray 2, the limiting frame 3 is rotated and placed flat on the tray 2, so that the neck of the shake flask passes through the limiting holes 41 formed by the elastic bands 4. The elastic bands 4 restrict the neck of the shake flask around its neck, which can effectively prevent the shake flask from moving under the inertial force when the tray 2 stops suddenly, thus avoiding the phenomenon of unstable placement of the shake flask and collision between multiple shake flasks.

[0021] By removing the elastic band 4 and installing it at different positions on the limiting frame 3, shaking flasks and beakers of different sizes can be limited, thus improving the applicability. Specifically, multiple limiting pins 31 are fixedly arranged along the circumference of the edge of the limiting frame 3, and connecting buckles 42 are fixedly arranged at both ends of the elastic band 4. The connecting buckles 42 are movably fastened to two adjacent limiting pins 31. Under the elastic force of the elastic band 4, the connecting buckles 42 on both sides are pulled to hook tightly onto the limiting pins 31, realizing the quick assembly and disassembly of the elastic band 4. Furthermore, each limiting pin 31 can accommodate at least two connecting buckles 42 vertically, thereby achieving the minimum distance between two adjacent elastic bands 4 being the distance between two adjacent limiting pins 31, which is suitable for limiting shaking flasks placed in different positions. In this embodiment, support frames 21 are further fixedly arranged vertically on both sides of the tray 2. One end of the limiting frame 3 is hinged to the support frame 21, and the other end is magnetically connected to the support frame 21. By magnetically connecting the movable end of the limiting frame 3 to the support frame 21, the limiting frame 3 can be quickly and easily fixed, improving the efficiency of the limiting frame 3's operation.

[0022] Furthermore, several anti-slip parts 22 are provided on the tray 2 directly below the limiting frame 3. The anti-slip parts 22 can be made of rubber. By setting the anti-slip parts 22, the stability of the shaker when placed on the tray 2 can be improved, and the phenomenon of the shaker sliding on the tray 2 during sudden stops can be reduced.

[0023] Furthermore, the multiple anti-slip parts 22 are arranged in a rectangular array on the tray 2, and a limiting groove 23 for inserting the bottom of the test tube is formed between four adjacent rectangular anti-slip parts 22. When the culture medium contained in the test tube is shaken, the bottom end of the test tube is inserted into the limiting groove 23 between the anti-slip parts 22, and the top end of the test tube is limited by the elastic band 4, which can effectively support and limit the test tube, and improve the applicability of the device.

[0024] Those skilled in the art will understand that although preferred embodiments of the present invention have been described, those skilled in the art, once they understand the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the present invention. Clearly, those skilled in the art can make various alterations and modifications to the present invention without departing from its spirit and scope. Thus, if these modifications and modifications of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include these modifications and modifications.

Claims

1. A shaker for suspension cell culture, characterized by: Including the cradle main part (1), be provided with the culture room (11) in the cradle main part (1), be provided with the tray (2) for placing the flask in the culture room (11), be provided with the drive assembly for driving the tray (2) reciprocating in the cradle main part (1), the upper parallel of the tray (2) is provided with the limiting frame (3), the limiting frame (3) is detachably provided with a plurality of elastic bands (4) on, a plurality of elastic bands (4) are used to constitute a plurality of limiting holes (41) for the flask neck to pass through on the limiting frame (3), one side edge of the limiting frame (3) is hinged with the tray (2), and the other side edge is detachably connected with the tray (2).

2. The shaker for suspension cell culture according to claim 1, wherein, The edge of the limiting frame (3) is fixedly provided with a plurality of limiting nails (31) along the circumference thereof, and the two ends of the elastic band (4) are fixedly provided with connecting buckles (42), which are movably buckled with the adjacent two limiting nails (31).

3. The shaker for suspension cell culture according to claim 2, wherein, Each of the limiting nails (31) can vertically accommodate at least two connecting buckles (42) buckled.

4. The shaker for suspended cell culture according to claim 1, wherein, The two side edges of the tray (2) are vertically fixedly provided with support frames (21), one end of the limiting frame (3) is hinged with the support frame (21), and the other end is magnetically connected with the support frame (21).

5. The shaker for suspended cell culture according to claim 1, wherein, The tray (2) is provided with a plurality of anti-skid parts (22), and the anti-skid part (22) is made of rubber material.

6. The shaker for suspended cell culture according to claim 5, wherein, A plurality of anti-skid parts (22) are arranged in a rectangular array on the tray (2), and a limiting groove (23) for inserting the bottom of a test tube is formed between four adjacent anti-skid parts (22) arranged in a rectangular shape.

7. The shaker for suspended cell culture according to claim 1, wherein, The drive assembly comprises a transmission frame (51) and a drive motor (52), at least three turntables (53) are rotatably arranged on the inner bottom wall of the culture room (11), a connecting column (54) is eccentrically arranged on the top wall of each turntable (53), the transmission frame (51) is fixedly arranged on the bottom wall of the tray (2), the connecting column (54) is rotatably connected with the transmission frame (51), and the drive motor (52) is used to drive any one of the turntables (53) to rotate.

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