Error-proof sample adding device for enzyme-linked immunosorbent assay

Abstract

The utility model relates to an enzyme linked immunosorbent assay (ELISA) mistake-proofing sample adding device, which belongs to the technical field of clinical immunological examination and comprises an ELISA seat frame plate, and a mounting structure for mounting an identification block is arranged above the ELISA seat frame plate; the mounting structure comprises a transverse plate, a telescopic frame and a sliding block, a first mounting groove and a second mounting groove are formed in the transverse plate, and an elastic piece for adjusting the transverse plate is arranged in the first mounting groove; the elastic piece is composed of an installation base, a guide block, a first reset spring and a guide rod, and the telescopic frame is fixedly installed on the outer surface of the guide block. According to the mistake-proof sample adding device for the enzyme-linked immunosorbent assay, two telescopic frames are pulled to expand outwards, a sliding block is connected with a sliding groove through the deformation effect of a first reset spring, and a transverse plate can be tightly attached to and fixed to ELISA seat frame plates with different diameters through the design, so that the application range and the flexibility of the device are greatly improved, and the purposes of high flexibility, high efficiency and the like are achieved. And the practicability is high.

Description

Technical Field

[0001] This utility model relates to the field of clinical immunology testing technology, specifically to an enzyme-linked immunosorbent assay (ELISA) device for preventing incorrect sample addition. Background Technology

[0002] Enzyme-linked immunosorbent assay (ELISA) uses a specific reaction between antigen and antibody to link the analyte to an enzyme, followed by a color reaction between the enzyme and substrate for quantitative determination. The most commonly used carrier is a microplate, and products specifically designed for ELISA assays are also called ELISA plates. The internationally accepted standard plate size is 8×12 with 96 wells, allowing for the simultaneous testing of 96 samples. For easier testing of small numbers of samples, 8- or 12-strand micro-reaction strips are available, which, when placed in an ELISA holder, are the same size as the standard ELISA plate. General kit ELISA holders lack special markings, making it easy to add the wrong or missing samples during clinical practice or demonstrations, leading to inaccurate test results.

[0003] A search revealed that patent document CN207992224U discloses an ELISA (Enzyme-Linked Immunosorbent Assay) error-proof sample dispensing device, comprising an ELISA holder plate and a horizontal slide plate. The ELISA holder plate is provided with 96 micro-reaction well fixing grids in an 8×12 grid. The horizontal slide plate includes a horizontal plate and two vertical plates. A vertical plate is fixed to the lower end of both the left and right sides of the horizontal plate. The left and right side walls of the ELISA holder plate are provided with grooves. The inner side of the vertical plates is provided with sliders that match the grooves. The sliders are slidably connected to the grooves. The upper surface of the horizontal plate is provided with 12 marking holes from left to right. Each marking hole corresponds to a sample dispensing well. The 12 marking holes are marked with numbers 1-12 from left to right.

[0004] The above-mentioned patent has the following shortcomings: the device may only be applicable to a specific model of ELISA stand plate. Different skateboards may need to be customized for different specifications or brands of stand plates, which limits its versatility and makes the device relatively inflexible. Therefore, an enzyme-linked immunosorbent assay (ELISA) error-proof sample addition device is proposed to solve the problems mentioned above. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides an error-proof sample addition device for enzyme-linked immunosorbent assay (ELISA), which has the advantages of high flexibility and strong practicality, and solves the problem that different slides may need to be customized for different specifications or brands of seat boards.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an enzyme-linked immunosorbent assay (ELISA) error-proof sample addition device, comprising an ELISA mounting plate, wherein an installation structure for mounting an identifier block is provided above the ELISA mounting plate;

[0007] The mounting structure includes a horizontal plate, a telescopic frame, and a slider. The horizontal plate has a first mounting groove and a second mounting groove inside. The first mounting groove is provided with an elastic element for adjusting the horizontal plate.

[0008] The elastic element consists of a mounting base, a guide block, a first reset spring, and a guide rod. The telescopic frame is fixedly installed on the outer surface of the guide block, the mounting base is fixed in the first mounting groove, and the guide rod is fixed between the mounting base and the inner wall of the first mounting groove. The guide rod passes through the interior of the guide block, and the first reset spring is fixed between the mounting base and the guide block.

[0009] The outer surface of the marker block is provided with a fixing structure for installation in the second mounting groove. The fixing structure includes a clamping plate, a guide rod, and a second return spring. The second return spring is fixed between the marker block and the clamping plate. One end of the guide rod is fixed to the outer surface of the clamping plate, and the other end of the guide rod extends into the interior of the marker block.

[0010] Furthermore, the ELI SA holder plate has sliding grooves on both the left and right sides that are adapted to the slider, and the upper left surface of the ELI SA holder plate is provided with a positioning mark groove. The upper surface of the ELI SA holder plate is U-shaped, and a micro-reaction hole fixing grid corresponding to the mark block is provided on its concave surface.

[0011] Furthermore, the second mounting groove is located on the upper surface of the horizontal plate, and a disassembly groove for removing the marker block is provided in the second mounting groove.

[0012] Furthermore, the telescopic frame consists of two telescopically connected vertical plates, and each of the two vertical plates has an adjustment telescopic hole, in which a positioning bolt can be detachably installed.

[0013] Furthermore, the telescopic frame, elastic element, and slider are all in pairs, with the two sliders slidably connected to the two slide grooves respectively.

[0014] Furthermore, the first reset spring surrounds the outer surface of the guide rod, and the second reset spring surrounds the outer surface of the guide rod.

[0015] Furthermore, the number of fixed structures on the identification block is two, and the two fixed structures are arranged symmetrically.

[0016] Compared with the prior art, this utility model provides an enzyme-linked immunosorbent assay (ELISA) device to prevent incorrect sample addition, which has the following beneficial effects:

[0017] 1. This enzyme-linked immunosorbent assay (ELISA) error-proof sample addition device expands outward by pulling two telescopic frames and uses the deformation effect of the first reset spring to connect the slider with the slide groove. This design allows the horizontal plate to fit tightly against and be fixed to ELISA base plates of different diameters, thereby greatly improving its applicability and flexibility. It achieves the advantages of high flexibility and strong practicality, and solves the problem that different slide plates may need to be customized for base plates of different specifications or brands.

[0018] 2. This enzyme-linked immunosorbent assay (ELISA) error-proof sample addition device uses two clamping blocks to press them closer to the label block. After the clamping blocks retract, the label block is placed into the second mounting slot. The deformation force of the second reset spring is used to firmly lock it into the horizontal plate, achieving the advantage of easy assembly and disassembly.

[0019] 3. This enzyme-linked immunosorbent assay (ELISA) error-proof sample dispensing device, by placing micro-reaction wells in the micro-reaction well fixed grid and using the digital identification blocks on the horizontal plate, makes it easy to distinguish the micro-reaction wells in each row of the ELISA holder plate during sample dispensing, so as to avoid dispensing the wrong sample and achieve the effect of preventing the wrong sample dispensing. Attached Figure Description

[0020] Figure 1 This is a three-dimensional view of the structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the structure of the EL I SA support plate of this utility model;

[0022] Figure 3 This is a schematic diagram of the installation structure of this utility model;

[0023] Figure 4 This utility model Figure 1 A magnified structural diagram of structure A is shown below;

[0024] Figure 5 This is a schematic diagram of the structure of the identification block of this utility model;

[0025] Figure 6 This is a schematic diagram of the fixed structure of this utility model.

[0026] In the diagram: 1. ELISA holder plate; 11. Slide groove; 12. Positioning mark groove; 13. Micro-reaction orifice fixing grid; 2. Mounting structure; 201. Horizontal plate; 202. Telescopic frame; 203. Slider; 204. Positioning bolt; 205. First mounting slot; 206. Mounting base; 207. Guide block; 208. First return spring; 209. Guide rod; 210. Second mounting slot; 211. Disassembly slot; 3. Marking block; 4. Fixing structure; 401. Clamping plate; 402. Guide rod; 403. Second return spring. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] Please see Figures 1 to 6 An ELISA (Enzyme-Linked Immunosorbent Assay) error-proof sample addition device includes an ELISA holder plate 1. An mounting structure 2 for mounting a marker block 3 is disposed above the ELISA holder plate 1. The mounting structure 2 includes a horizontal plate 201, a telescopic frame 202, and a slider 203. The horizontal plate 201 has a first mounting groove 205 and a second mounting groove 210 inside. An elastic element for adjusting the horizontal plate 201 is disposed in the first mounting groove 205. The elastic element consists of a mounting base 206, a guide block 207, a first return spring 208, and a guide rod 209. Slide grooves 11 adapted to the slider 203 are provided on both the left and right sides of the ELISA holder plate 1. A positioning marker groove 12 is provided on the upper left surface of the ELISA holder plate 1. The upper surface of the ELISA holder plate 1 is U-shaped, and a micro-reaction hole fixing grid 13 corresponding to the marker block 3 is provided on its concave surface. A roller is rotatably mounted on the side of the slider 203 near the slide groove 11, enabling better movement of the horizontal plate 201.

[0029] Specifically, the telescopic bracket 202 is fixedly installed on the outer surface of the guide block 207, the mounting base 206 is fixed inside the first mounting groove 205, and the guide rod 209 is fixed between the mounting base 206 and the inner wall of the first mounting groove 205, the guide rod 209 passing through the interior of the guide block 207, and the first return spring 208 is fixed between the mounting base 206 and the guide block 207. The second mounting groove 210 is located on the upper surface of the horizontal plate 210. By pulling the two telescopic brackets 202, the two telescopic brackets 202 expand outward, and the deformation effect of the first return spring 208 connects the slider 203 with the slide groove 11. This method allows the horizontal plate 201 to be adapted to ELI SA bracket plates 1 of different diameters, effectively improving the installation flexibility and practicality of the marking block 3.

[0030] In this embodiment, the outer surface of the marker block 3 is provided with a fixing structure 4 for installation in the second mounting groove 210. The fixing structure 4 includes a clamping plate 401, a guide rod 402, and a second return spring 403. The second return spring 403 is fixed between the marker block 3 and the clamping plate 401. One end of the guide rod 402 is fixed to the outer surface of the clamping plate 401, and the other end of the guide rod 402 extends into the interior of the marker block 3. A disassembly groove 211 for disassembling the marker block 3 is provided in the second mounting groove 210.

[0031] It should be noted that the telescopic frame 202 consists of two telescopically connected vertical plates, and each vertical plate has an adjustment telescopic hole, in which a positioning bolt 204 can be detachably installed. There are two telescopic frames 202, two elastic elements, and two sliders 203, with each slider 203 slidably connected to two sliding grooves 11. Through the installation and cooperation of the telescopic frame 202 and the positioning bolts 204, the height of the horizontal plate 201 can be adjusted, allowing workers to perform sampling operations more effectively.

[0032] The first return spring 208 surrounds the outer surface of the guide rod 209, and the second return spring 403 surrounds the outer surface of the guide rod 402. There are two fixing structures 4 on the marker block 3, and the two fixing structures 4 are symmetrically arranged. The marker block 3 is installed using the fixing structures 4, which makes the installation and removal of the marker block 3 more flexible. The marker block 3 can be installed inside the horizontal plate 201 as needed, improving the installation flexibility of the marker block 3.

[0033] The working principle of the above embodiments is as follows:

[0034] In use, by pulling the two telescopic brackets 202, the two telescopic brackets 202 expand outward. Utilizing the deformation effect of the first return spring 208, the slider 203 is connected to the slide groove 11. This method allows the horizontal plate 201 to be adapted to ELISA bracket plates 1 of different diameters, effectively improving the installation flexibility and practicality of the marking block 3.

[0035] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An enzyme-linked immunosorbent assay (ELISA) mistake proofing pipetting device comprising an ELISA rack plate (1), characterized in that: The upper part of the ELISA frame plate (1) is provided with a mounting structure (2) for mounting an identification block (3); The mounting structure (2) comprises a horizontal plate (201), an extension frame (202) and a sliding block (203), the inside of the horizontal plate (201) is provided with a first mounting groove (205) and a second mounting groove (210), the first mounting groove (205) is provided with an elastic member for adjusting the horizontal plate (201); The elastic member is composed of a mounting seat (206), a guide block (207), a first reset spring (208) and a guide rod (209), the extension frame (202) is fixedly installed on the outer surface of the guide block (207), the mounting seat (206) is fixed in the first mounting groove (205), and the guide rod (209) is fixed between the mounting seat (206) and the inner wall of the first mounting groove (205), the guide rod (209) penetrates the inside of the guide block (207), and the first reset spring (208) is fixed between the mounting seat (206) and the guide block (207); The outer surface of the identification block (3) is provided with a fixing structure (4) for mounting in the second mounting groove (210), the fixing structure (4) comprises a clamping plate (401), a guide rod (402) and a second reset spring (403), the second reset spring (403) is fixed between the identification block (3) and the clamping plate (401), one end of the guide rod (402) is fixed to the outer surface of the clamping plate (401), and the other end of the guide rod (402) extends into the inside of the identification block (3).

2. The ELISA mistake proofing pipetting device of claim 1, wherein: The left and right sides of the ELISA frame plate (1) are both provided with a sliding groove (11) matched with the sliding block (203), the left upper surface of the ELISA frame plate (1) is provided with a positioning mark groove (12), the upper surface of the ELISA frame plate (1) is in a concave shape, and the concave surface is provided with a micro-reaction hole fixing grid (13) corresponding to the identification block (3).

3. The ELISA mistake proofing pipetting device of claim 1, wherein: The second mounting groove (210) is located on the upper surface of the horizontal plate, and the second mounting groove (210) is provided with a dismounting groove (211) for dismounting the identification block (3).

4. The ELISA mistake proofing pipetting device of claim 1, wherein: The extension frame (202) is composed of two vertically arranged extension frames, and the two vertically arranged extension frames are both provided with an adjusting extension hole, and the adjusting extension hole is detachably provided with a positioning bolt (204).

5. The ELISA mistake proofing pipetting device of claim 2, wherein: The number of the extension frame (202), the elastic member and the sliding block (203) is two, and the two sliding blocks (203) are respectively connected with the two sliding grooves (11).

6. The ELISA mistake proofing pipetting device of claim 1, wherein: The first reset spring (208) surrounds the outer surface of the guide rod (209), and the second reset spring (403) surrounds the outer surface of the guide rod (402).

7. The ELISA mistake proofing pipetting device of claim 1, wherein: The number of the fixing structure (4) on the identification block (3) is two, and the two fixing structures (4) are symmetrically arranged.

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