Refrigeration equipment applied to cell culture

Abstract

The utility model belongs to refrigerating equipment applied to cell culture, and particularly relates to the technical field of cell culture.The refrigerating equipment comprises a base, a holding rod and a shell are connected to the upper portion of the base from left to right, nine dampers are connected to the bottom face of the inner wall of the shell in an array mode, and a sealing box is connected to the tops of the dampers; a sealing plate is in lap joint with the top of the sealing box, five brackets are sequentially connected to the interior of the sealing box from top to bottom, four limiting rings are connected to the upper portions of the brackets, culture dishes are connected to the inner walls of the limiting rings, and a nitrile rubber ring at the bottom of the previous bracket is extruded on the culture dish of the next bracket; according to the design, the adjacent culture dishes are in close contact through the nitrile rubber pads, meanwhile, the sealing plate is arranged at the top of the uppermost bracket, and the nitrile rubber pads arranged at the bottom of the sealing plate extrude the tops of the culture dishes, so that the overall sealing performance is further enhanced, and the sealing effect cannot be influenced by shaking or vibration.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, specifically relating to a refrigeration device used in cell culture. Background Technology

[0002] Cell culture is a technique that simulates the in vivo environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structure and function. It has a wide range of applications in life science research, biopharmaceuticals, cell therapy, and other fields. Cell culture media, serum, enzymes, and other biological agents usually need to be refrigerated. These substances are prone to deterioration, degradation, or loss of activity at room temperature. Refrigeration can maintain their stability and extend their shelf life.

[0003] Current cell culture refrigeration equipment has poor sealing performance during use. Since cells are generally cultured in containers such as culture flasks and culture dishes, even if these containers are well sealed under normal conditions, large bumps during transportation may cause the container lids to loosen. Once the culture medium leaks, the cells will be exposed to an unsuitable environment, affecting their activity and survival. Utility Model Content

[0004] The purpose of this invention is to provide a refrigeration device for cell culture that can solve the problems mentioned in the background art.

[0005] The specific technical solution adopted in this utility model is as follows:

[0006] A refrigeration device for cell culture includes a base with four casters attached to its lower surface. A handle and a housing are attached to the upper surface of the base from left to right. A cover plate is rotatably connected to one side of the housing. Nine dampers are arrayed on the bottom inner wall of the housing. A sealing box is connected to the top of each damper. A pair of snap-on plates are attached to both sides of the top of the sealing box. A sealing strip is fixedly connected to the top of the sealing box. A sealing plate overlaps the top of the sealing strip. A pair of slots are provided on both sides of the sealing plate. The top of the snap-on plates is embedded into the slots. A first sealing mechanism is provided at the bottom of the sealing plate.

[0007] The sealed box has five brackets connected from top to bottom inside. Each bracket has a guide mechanism on both sides. Four limiting rings are connected to the top of the bracket. The inner wall of the limiting rings is provided with a buffer mechanism. The inner wall of the buffer mechanism is connected with a petri dish. The bottom of the bracket is provided with a second sealing mechanism.

[0008] A fixing seat is fixedly connected to the left side wall of the base, a liquid nitrogen tank is connected above the fixing seat, a hose is connected to the top of the liquid nitrogen tank, one end of the hose extends into the inside of the sealed box, and a valve is connected to the outside of the hose.

[0009] The present invention is further configured such that: the first sealing mechanism includes four nitrile rubber pads, the positions of which correspond to the positions of the culture dishes.

[0010] The present invention is further configured such that the second sealing mechanism includes four nitrile rubber rings, the positions of which correspond to the positions of the culture dish.

[0011] This utility model is further configured such that the outer diameters of the nitrile rubber ring and the nitrile rubber pad are both the same, and both are equal to the outer diameter of the culture dish.

[0012] The present invention is further configured such that the buffer mechanism includes seven nitrile rubber strips, which are arranged in a circumferential direction on the inner wall of the limiting ring.

[0013] The present invention is further configured such that: the guiding mechanism includes a side plate, one side of the side plate is fixedly connected to the bracket, a pair of guide posts are slidably connected inside the side plate, and the ends of the guide posts are fixedly connected to the bottom surface of the inner wall of the sealing box.

[0014] The present invention is further configured such that: a controller is connected to the front side of the sealed box, a temperature sensor is connected to the side wall of the sealed box, and the temperature sensor is connected to the input terminal of the controller.

[0015] The technical effects achieved by this utility model are as follows:

[0016] This utility model relates to a refrigeration device for cell culture. Nine dampers are installed on the bottom inner wall of the outer shell. These nine dampers, distributed on the bottom surface, support the sealed container from multiple points, effectively absorbing and dissipating the vibration energy generated when the outer shell is subjected to external shocks during transportation. This reduces the impact on the culture dishes and protects their stability. Five trays are sequentially inserted into the guide columns on both sides. The nitrile rubber ring at the bottom of the upper tray presses against the culture dish on the next tray. This design ensures close contact between adjacent culture dishes through the nitrile rubber pads, achieving a sealing effect. Simultaneously, a sealing plate is installed at the top of the uppermost tray. The nitrile rubber pad at the bottom of the sealing plate presses against the top of the culture dish, further enhancing the overall sealing performance. The sealing plate, acting as a top barrier, combined with the compression of the nitrile rubber pads, forms a closed space, ensuring that the sealing effect is not affected by shaking or vibration during transportation or other conditions. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this practical tool;

[0018] Figure 2 This is a side sectional view of the outer shell of this utility model;

[0019] Figure 3This is an exploded view of the sealing plate and sealing box in this utility model;

[0020] Figure 4 This is a side sectional view of the sealing box in this utility model;

[0021] Figure 5 This is an exploded view of the bracket and petri dish in this utility model;

[0022] Figure 6 This is a bottom view of the sealing plate in this utility model;

[0023] Figure 7 This is a bottom view of the bracket in this utility model.

[0024] In the diagram: 1. Outer shell; 2. Sealing plate; 3. Cover plate; 4. Base; 5. Casters; 6. Sealing box; 7. Handle bar; 8. Buckle plate; 9. Valve; 10. Hoses; 11. Liquid nitrogen tank; 12. Fixing base; 13. Damper; 14. Controller; 15. Slot; 16. Limiting ring; 17. Guide post; 18. Side plate; 19. Bracket; 20. Petri dish; 21. Temperature sensor; 22. Sealing strip; 23. Nitrile rubber strip; 24. Nitrile rubber pad; 25. Nitrile rubber ring; Detailed Implementation

[0025] To make the purpose and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific implementations of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0026] like Figure 1-7 As shown, a refrigeration device for cell culture includes a base 4, four casters 5 connected to the bottom of the base 4, a handle 7 and a housing 1 connected from left to right on the top of the base 4, a cover plate 3 rotatably connected to one side of the housing 1, nine dampers 13 arrayed on the bottom surface of the inner wall of the housing 1, a sealing box 6 connected to the top of the dampers 13, a pair of buckle plates 8 connected to both sides of the top of the sealing box 6, a sealing strip 22 fixedly connected to the top of the sealing box 6, and an overlap of the sealing strip 22. The sealing plate 2 has a pair of slots 15 on both sides. The top of the buckle plate 8 is embedded in the slot 15. The bottom of the sealing plate 2 is provided with a first sealing mechanism. The interior of the sealing box 6 is connected to five brackets 19 from top to bottom. The brackets 19 are provided with guide mechanisms on both sides. The brackets 19 are connected to four limiting rings 16. The inner wall of the limiting rings 16 is provided with a buffer mechanism. The inner wall of the buffer mechanism is connected to a petri dish 20. The bottom of the brackets 19 is provided with a second sealing mechanism.

[0027] like Figure 5-7As shown, a fixing seat 12 is fixedly connected to the left side wall of the base 4. A liquid nitrogen tank 11 is connected above the fixing seat 12. A hose 10 is connected to the top of the liquid nitrogen tank 11. One end of the hose 10 extends into the interior of the sealed box 6. A valve 9 is connected to the outside of the hose 10. The first sealing mechanism includes four nitrile rubber pads 24. The positions of the nitrile rubber pads 24 correspond to the positions of the culture dish 20. The nitrile rubber pads 24 set at the bottom of the sealing plate 2 are pressed against the top of the culture dish 20, further enhancing the overall sealing performance.

[0028] like Figure 6-7 As shown, the second sealing mechanism includes four nitrile rubber rings 25, the positions of which correspond to the positions of the culture dishes 20. This design allows adjacent culture dishes 20 to make close contact through the nitrile rubber rings 25, thus achieving a sealing effect. The outer diameters of the nitrile rubber rings 25 and the nitrile rubber pads 24 are the same and equal to the outer diameter of the culture dishes 20, which is beneficial for achieving tight pressure on the culture dishes 20.

[0029] like Figure 5 As shown, the buffer mechanism includes seven nitrile rubber strips 23, which are arranged in a ring on the inner wall of the limiting ring 16. Nitrile rubber has good low-temperature resistance and can maintain a certain elasticity and flexibility in the low-temperature environment of liquid nitrogen tank 11. It is not easy to become brittle and can effectively play a buffering role.

[0030] like Figure 4-5 As shown, the guide mechanism includes a side plate 18, one side of which is fixedly connected to the bracket 19. A pair of guide posts 17 are slidably connected inside the side plate 18. The ends of the guide posts 17 are fixedly connected to the bottom surface of the inner wall of the sealing box 6, which helps to guide and limit the bracket 19.

[0031] like Figure 3-4 As shown, a controller 14 is connected to the front of the sealed box 6, and a temperature sensor 21 is connected to the side wall of the sealed box 6. The temperature sensor 21 is connected to the input terminal of the controller 14. By setting the temperature sensor 21, it is convenient to monitor the temperature inside the sealed box 6. When the temperature is high, the controller 14 controls the opening of the liquid nitrogen tank 11 to cool down the temperature inside the sealed box 6. When the temperature inside the sealed box 6 drops to the set value, the controller 14 controls the closing of the liquid nitrogen tank 11, which achieves the effect of heat preservation.

[0032] The working principle of this utility model is as follows: During use, nine dampers 13 are set on the bottom surface of the inner wall of the outer shell 1. The nine dampers 13 are distributed on the bottom surface, which can support the sealed box 6 from multiple points. This can effectively absorb and dissipate the vibration energy generated by the outer shell 1 when it is subjected to external vibration during transportation, reduce the impact on the culture dish 20, and protect the stability of the culture dish 20. Five brackets 19 are inserted into the guide columns 17 on both sides in sequence. The nitrile rubber ring 25 at the bottom of the upper bracket 19 is pressed against the culture dish 20 of the next bracket 19. This design allows the adjacent culture dishes 20 to make close contact through the nitrile rubber ring 25, which plays a sealing role. At the same time, a sealing plate 2 is set on the top of the uppermost bracket 19. The nitrile rubber pad 24 at the bottom of the sealing plate 2 is pressed against the top of the culture dish 20, which further enhances the overall sealing performance. The sealing plate 2, as the top barrier of the whole, together with the compression of the nitrile rubber pad 24, forms a closed space to ensure that the sealing effect will not be affected by shaking or vibration during transportation or other situations.

[0033] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the art.

Claims

1. A refrigeration device for cell culture, characterized in that: include: The base (4) has four casters (5) connected to its bottom. The base (4) has a handle (7) and a housing (1) connected from left to right to its top. A cover plate (3) is rotatably connected to one side of the housing (1). Nine dampers (13) are arrayed on the bottom surface of the inner wall of the housing (1). A sealing box (6) is connected to the top of the damper (13). A pair of buckles (8) are connected to both sides of the top of the sealing box (6). A sealing strip (22) is fixedly connected to the top of the sealing box (6). A sealing plate (2) overlaps the top of the sealing strip (22). A pair of slots (15) are opened on both sides of the sealing plate (2). The top of the buckle (8) is embedded in the slot (15). A first sealing mechanism is provided at the bottom of the sealing plate (2). The sealed box (6) has five brackets (19) connected from top to bottom inside. Each bracket (19) has a guide mechanism on both sides. Each bracket (19) has four limiting rings (16) connected above it. Each limiting ring (16) has a buffer mechanism on its inner wall. Each buffer mechanism has a petri dish (20) connected to its inner wall. Each bracket (19) has a second sealing mechanism at its bottom. A fixed seat (12) is fixedly connected to the left side wall of the base (4). A liquid nitrogen tank (11) is connected above the fixed seat (12). A hose (10) is connected to the top of the liquid nitrogen tank (11). One end of the hose (10) extends into the interior of the sealed box (6). A valve (9) is connected to the outside of the hose (10).

2. The refrigeration equipment for cell culture according to claim 1, characterized in that: The first sealing mechanism includes four nitrile rubber pads (24), the positions of which correspond to the positions of the petri dishes (20).

3. The refrigeration equipment for cell culture according to claim 2, characterized in that: The second sealing mechanism includes four nitrile rubber rings (25), the positions of which correspond to the positions of the culture dish (20).

4. A refrigeration device for cell culture according to claim 3, characterized in that: The outer diameters of the nitrile rubber ring (25) and the nitrile rubber pad (24) are the same and are equal to the outer diameter of the culture dish (20).

5. A refrigeration device for cell culture according to claim 1, characterized in that: The buffer mechanism includes seven nitrile rubber strips (23), which are arranged in a circumferential direction on the inner wall of the limiting ring (16).

6. A refrigeration device for cell culture according to claim 1, characterized in that: The guiding mechanism includes a side plate (18), one side of which is fixedly connected to a bracket (19), and a pair of guide posts (17) are slidably connected inside the side plate (18), the ends of which are fixedly connected to the bottom surface of the inner wall of the sealing box (6).

7. A refrigeration device for cell culture according to claim 1, characterized in that, A controller (14) is connected to the front side of the sealed box (6), and a temperature sensor (21) is connected to the side wall of the sealed box (6). The temperature sensor (21) is connected to the input terminal of the controller (14).

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