Cell carrier covered stent, carrier manufacturing device and carrier dehydration device
By designing cell carrier membrane scaffolds and corresponding carrier fabrication and dehydration devices, the complex problems of cell carrier movement and transfer during organoid manufacturing were solved, achieving efficient high-throughput culture and consistent manufacturing.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2026-04-07
AI Technical Summary
In existing technologies, the movement and transfer of cell carriers during organoid manufacturing are complex and difficult to meet the needs of high-throughput culture.
A cell carrier membrane scaffold was designed, including a scaffold body and a first support boss. Support arms are arranged in an array along the outer periphery of the scaffold body. Combined with a carrier fabrication device and a dehydration device, stable support and efficient transfer of cell carriers are achieved.
It simplifies the organoid manufacturing process, improves the mobility and transfer efficiency of cell vectors, meets the needs of high-throughput culture, and enhances the controllability and consistency of organoid manufacturing.
Smart Images
Figure CN224091894U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological tissue engineering technology, and in particular to a cell carrier membrane scaffold. Background Technology
[0002] Organoids are tissue models that mimic the characteristics of corresponding organs in vivo, obtained by culturing adult stem cells or pluripotent stem cells in vitro. They have broad prospects in basic research, disease modeling, drug development, and precision treatment.
[0003] High-throughput culture of organoids can be achieved through culture systems such as microwell arrays and microfluidic droplets. However, currently, the containers used to load cell carriers in organoid manufacturing are generally culture dishes, which are cumbersome to move and process, and cannot even meet the new process requirements of high-throughput culture. Therefore, it is necessary to develop a cell carrier-coated scaffold to help simplify the organoid manufacturing process. Utility Model Content
[0004] The technical problem to be solved by this invention is to provide a cell carrier membrane scaffold to help simplify the manufacturing process of organoids.
[0005] To solve the above-mentioned technical problems, this utility model provides a cell carrier membrane scaffold, including a scaffold body and a first supporting boss. The scaffold body is provided with a through hole, and the first supporting boss is arranged circumferentially along the through hole. The first supporting boss is used to support a first cell carrier.
[0006] As an improvement to the above solution, the top surface of the first support boss is provided with a preset distance from the top of the bracket body.
[0007] As an improvement to the above solution, a support arm is also included, which is arranged in an array along the outer periphery of the support body.
[0008] In addition, this utility model also provides a carrier manufacturing device, which includes an array mold, a support component, and the above-mentioned cell carrier coating scaffold. The coating scaffold is disposed in the support component, and the support component is provided with a second support boss. The second support boss is adapted to the through hole and extends from the bottom of the through hole to form a receiving cavity. The receiving cavity is provided with an inert gel that contacts the first support boss. The bottom of the array mold is provided with a protrusion array, and the protrusion array forms a concave hole array on the top surface of the inert gel.
[0009] As an improvement to the above solution, the support assembly includes a petri dish and a support, the support being disposed in the petri dish, and the second support boss being disposed on the support.
[0010] As an improvement to the above solution, the support also includes a base and a limiting boss. The second supporting boss and the limiting boss are both formed on the top of the base. The limiting boss is arranged around the outer periphery of the second supporting boss. The main body of the bracket is located between the limiting boss and the second supporting boss.
[0011] As an improvement to the above solution, the protrusion array is disposed opposite to the second support protrusion and offset from the first support protrusion.
[0012] As an improvement to the above scheme, the protrusion array is conical.
[0013] In addition, this utility model also provides a carrier dehydration device, which includes a dehydration module and the above-mentioned cell carrier membrane support. The dehydration module is provided with a dehydration channel, and the end of the dehydration module is provided with a rotationally symmetrical mounting groove. The mounting groove is correspondingly provided with the support arm. The support arm is screwed into the mounting groove, and the support body extends into the dehydration channel.
[0014] As an improvement to the above solution, the mounting groove is spirally arranged, and the support arm is arranged along the spiral direction of the mounting groove on the outer periphery of the bracket body.
[0015] Implementing this utility model has the following beneficial effects:
[0016] This invention discloses a cell carrier membrane scaffold. By setting a through hole in the scaffold body, a first supporting boss is arranged circumferentially along the through hole. The first supporting boss can support the first cell carrier, facilitating the movement of the first cell carrier with the cell carrier membrane scaffold. At the same time, the combination of the through hole and the first supporting boss exposes the top and bottom surfaces of the first cell carrier to the outside, facilitating the subsequent formation and dehydration of the concave array of the first cell carrier. The cell carrier membrane scaffold of this invention does not require the transfer of the first cell carrier between different containers to meet the requirements of the new process, simplifying the fabrication and transfer operation of the first cell carrier, and thus helping to simplify the manufacturing process of organoids. Attached Figure Description
[0017] Figure 1 This is a schematic diagram of an embodiment of a cell carrier membrane scaffold according to the present invention;
[0018] Figure 2 This is a schematic diagram of an embodiment of a carrier manufacturing device according to the present invention;
[0019] Figure 3 yes Figure 2 A schematic diagram of the support structure;
[0020] Figure 4 This is a schematic diagram of an embodiment of the carrier dehydration device of this utility model;
[0021] Figure 5 yes Figure 4 Side view;
[0022] Figure 6 yes Figure 5 A schematic diagram of the CC section structure;
[0023] Figure 7 This is a schematic diagram of the dehydration device, the second cell carrier, and the second membrane-covered scaffold.
[0024] Figure 8 This is a schematic diagram of the assembly structure of the dehydration platform and dehydration module;
[0025] Figure 9 yes Figure 8 Top view;
[0026] Figure 10 yes Figure 9 A schematic diagram of the DD cross-section structure.
[0027] Figure 11 This is a schematic diagram of an automated manufacturing system for cell trap arrays that utilizes a human-computer interaction mechanism, including a cell carrier membrane scaffold, a carrier fabrication device, and a carrier dehydration device.
[0028] Figure 12 yes Figure 11 A structural diagram of the workbench, robotic arm, and industrial camera; Detailed Implementation
[0029] To make the objectives, technical solutions and advantages of this utility model clearer, the utility model will be described in further detail below with reference to the accompanying drawings.
[0030] like Figure 1 As shown, this utility model discloses an embodiment of a cell carrier membrane scaffold, including a scaffold body 121 and a first supporting boss 122. The scaffold body 121 is provided with a through hole 123, and the first supporting boss 122 is arranged circumferentially along the through hole 123. The first supporting boss 122 is used to support a first cell carrier b.
[0031] In this embodiment, by providing a through hole 123 in the scaffold body 121, the first support boss 122 is arranged circumferentially along the through hole. The first support boss 122 can support the first cell carrier b, facilitating the movement of the first cell carrier b with the cell carrier-coated scaffold. At the same time, the combination of the through hole 123 and the first support boss 122 exposes both the top and bottom surfaces of the first cell carrier b to the outside, facilitating the subsequent formation and dehydration of the concave array of the first cell carrier b. The cell carrier-coated scaffold of this embodiment does not require the transfer of the first cell carrier b between different containers to meet the requirements of the new process, simplifying the fabrication and transfer operation of the first cell carrier b, and thus helping to simplify the manufacturing process of organoids.
[0032] In this embodiment, the first cell carrier b is made of inert gel a, preferably agarose gel. The top surface of the first support protrusion 122 is provided with a preset distance from the top of the support body 121, so that the agarose poured into the through hole 123 can cover the first support protrusion 122 after solidification, so that the first support protrusion 122 can achieve the effect of embedding into the agarose gel and form a stable support for it.
[0033] To facilitate the movement of the cell carrier membrane scaffold 12 and the first cell carrier b on it, the cell carrier membrane scaffold 12 in this embodiment is also provided with support arms 124, which are arranged in an array along the outer periphery of the scaffold body 121.
[0034] In addition, combined Figure 2 and Figure 3 The present invention also provides a carrier manufacturing device, which includes an array mold 11, a support assembly, and the aforementioned cell carrier membrane scaffold 12. The cell carrier membrane scaffold 12 is disposed within the support assembly. The support assembly is provided with a second support boss 161, which is adapted to a through hole 123. The second support boss 161 extends from the bottom through hole 123 and forms a receiving cavity with the through hole 123. An inert gel a is disposed in the receiving cavity and contacts the first support boss 122. The bottom of the array mold 11 is provided with a protrusion array, which forms a concave hole array b1 on the top surface of the inert gel a.
[0035] In this embodiment, after agarose is poured into the cavity, the agarose forms an agarose gel. The agarose gel covers the first support protrusion 122, so that the first support protrusion 122 can be embedded in the agarose gel and form a stable support for it.
[0036] The support assembly in this embodiment specifically includes a second culture dish 17 and a support 16 disposed within the second culture dish 17. The support 16 includes a base 162, a limiting boss 163, and the aforementioned second supporting boss 161. Both the second supporting boss 161 and the limiting boss 163 are formed on the top of the base 162. The limiting boss 163 is arranged in an array around the outer periphery of the second supporting boss 161. The support body 121 is disposed between the limiting boss 163 and the second supporting boss 161, and the support arm 124 is disposed between two adjacent limiting bosses 163. The second culture dish 17 can collect substances overflowing during agarose casting. The structure of the support 16 allows for the directional placement of the cell carrier membrane scaffold 12, facilitating rapid positioning and pressing of the array mold 11 and convenient forming of the concave hole array b1. Furthermore, by designing a support 16 with second support protrusions 161 of varying heights, the height at which the second support protrusions 161 extend into the through-hole 123 can be altered, thereby changing the thickness of the agarose gel and adapting to different organoid culture needs. The support 16, in conjunction with the cell carrier coating scaffold 12, forms a cavity for containing agarose, enabling the acquisition of agarose gels of varying thicknesses without the need for separately designing different models of cell carrier coating scaffolds 12.
[0037] The bottom of the array mold 11 has a raised array corresponding to the concave array b1. The array mold 11 can directly press the concave array b1 onto the top surface of the inert gel a, or press it onto the top of the poured agarose. After solidification, the array mold 11 is removed to obtain an agarose gel with the concave array b1. When the array mold 11 presses the surface of the agarose gel, the raised array is opposite to the second support protrusion 161 and offset from the first support protrusion 122. After pressing, the array mold 11 is pulled out to form the concave array b1 in the middle area of the agarose gel surface. The agarose gel in the middle area has good uniformity in thickness, which is beneficial to improving the consistency of the cell trap. The agarose gel forming the concave array b1 is the first cell carrier b. By moving the cell carrier coating scaffold 12, the first cell carrier b can be transferred to the cell seeding area B for cell seeding.
[0038] The protrusions of the array mold 11 are conical, and the concave array b1 structure formed by the array mold 11 with this structure makes it easier for the cells inside to detach from the first cell carrier b.
[0039] In addition, combined Figures 4 to 7 The present invention also provides a carrier dehydration device, which includes a dehydration module 14 and the cell carrier membrane support 12. The dehydration module 14 is provided with a dehydration channel 141. The cell carrier membrane support 12 is detachably connected to the dehydration module 14. The first cell carrier b is at least partially exposed in the dehydration channel 141 for dehydration.
[0040] Specifically, the end of the dehydration module 14 is provided with a rotationally symmetrical mounting groove 142, which is correspondingly provided with the support arm 124. The support arm 124 is provided on the outer periphery of the bracket body 121 along the spiral direction of the mounting groove 142. The support arm 124 is screwed into the mounting groove 142, and the bracket body 121 extends into the dehydration channel 141.
[0041] In this embodiment, a second membrane scaffold 15 is preferably provided outside the first cell carrier b. A second cell carrier c is provided inside the second membrane scaffold 15. The second cell carrier c is opposite to the concave array b1 of the first cell carrier b. The second cell carrier c enters the concave array b1 and encapsulates the cells to form a cell trap array.
[0042] When performing cell seeding or dehydration, with the concave array b1 facing upwards, the support arm 124 of the cell carrier membrane scaffold 12 is screwed into the mounting groove 142, and the scaffold body 121 extends into the dehydration channel 141. At this time, the through hole 123 is coaxial with the dehydration channel 141, and the surface of the first cell carrier b opposite to the concave array b1 is exposed in the dehydration channel 141, realizing rapid dehydration of the first cell carrier b. At the same time, the second cell carrier c is dehydrated through the concave array. The top surface of the second cell carrier c is covered by the second membrane scaffold 15, and a large adsorption area is maintained between the second cell carrier c and the second membrane scaffold 15, which facilitates the subsequent separation of the second cell carrier c from the first cell carrier b.
[0043] The dehydration channel 141 includes a settling platform 144. The cell carrier membrane-coated scaffold 12 is spirally connected to the dehydration module 14. After the scaffold body 121 extends into the dehydration channel 141, it abuts against the settling platform 144, sealing the dehydration channel 141 and isolating the second cell carrier c from the dehydration channel 141. The cell carrier membrane-coated scaffold 12 not only facilitates movement and positioning installation but also controls the flow of heat sources.
[0044] The dehydration module 14 can be equipped with two or more dehydration channels 141, and the top of each dehydration channel 141 is connected to the cell carrier membrane scaffold 12 to achieve higher throughput cell seeding.
[0045] In this embodiment, the first cell carrier b is supported by the cell carrier membrane scaffold 12. The support arm 124 structure of the cell carrier membrane scaffold 12 cooperates with the spiral mounting groove 142 of the dehydration module 14, which facilitates the assembly and disassembly of the cell carrier membrane scaffold 12 and the dehydration module 14. The through hole 123 structure of the cell carrier membrane scaffold 12 can promote the dehydration of the first cell carrier b and the second cell carrier c.
[0046] Among them, combined Figures 8 to 10The dehydration module 14 is mounted on the dehydration platform 13. The dehydration platform 13 has a mounting cavity 132 and a dehydration hole 133. The mounting cavity 132 is used to accommodate the dehydration module 14, and the dehydration hole 133 is used to connect the heat source cavity 131 and the dehydration channel 141 so that the hot air in the heat source cavity 131 flows to the dehydration channel 141. The side wall of the mounting cavity 132 has a first limiting groove 134 and a second limiting groove 135. The second limiting groove 135 is connected to the bottom end of the first limiting groove 134, and the second limiting groove 135 extends from the first limiting groove 134 into the circumference of the mounting cavity 132. The second end of the dehydration module 14 has a mounting protrusion 143 protruding outward from the wall. The mounting protrusion 143 is inserted into the second limiting groove 135 from the top end of the first limiting groove 134.
[0047] The second cell carrier c is a biohydrogel such as matrix gel, collagen, or fibrinogen-thrombin; in this embodiment, matrix gel, commonly used in organoid culture, is preferred. The matrix gel encapsulates the cultured cells, resulting in a more uniform surrounding environment and preventing polarity reversal due to differences in micro-surrounding polarities, thus maximally mimicking the in vivo environment. The second membrane-covered scaffold 15 is preferably the first culture dish. The top surface of the second cell carrier c is covered by the first culture dish, and the first cell carrier b and the cell carrier membrane-covered scaffold 12 isolate the second cell carrier c from the dehydration channel 141. When the first culture dish containing matrix gel is placed on the agarose gel with the concave array b1, the matrix gel adheres to the first culture dish.
[0048] Due to its inert chemical properties, agarose gel cannot form covalent bonds with all current bio-hydrogels. During the research process, this invention discovered that many bio-hydrogels used for manufacturing organoids, including matrix gels, can form entangled states through dehydration, providing gels with strength exceeding that of cross-linked structures in a short time. Therefore, the second cell carrier c does not form chemical bonds upon contact with the first cell carrier b. Furthermore, in this embodiment, the second cell carrier c within the second membrane scaffold 15 is positioned opposite the pore array b1. During the dehydration of the first cell carrier b, the first cell carrier b and the cell carrier membrane scaffold 12 isolate the second cell carrier c from the dehydration channel 141. Although the second cell carrier c is not directly exposed to the dehydration channel 141, the pore array b1 structure on the first cell carrier b allows the second cell carrier c to also dehydrate, resulting in an entangled state on the side of the second cell carrier c opposite to the pore array b1. In this embodiment, as the second cell carrier c enters the concave array b1 and encapsulates the cells, the matrix gel, after dehydration and forming an entangled portion, increases its physical strength, transforming from a very soft state into a relatively hard solid, making it easier to separate from the first cell carrier b. When the side of the second cell carrier c opposite to the concave array b1 is dehydrated and entangled, the side of the second cell carrier c in contact with the first culture dish retains its original state; that is, the top of the second cell carrier c is adsorbed onto the first culture dish. A gentle pull by hand is sufficient to completely detach the matrix gel from the agarose gel, leaving the cell-encapsulated matrix gel on the first culture dish, thus completing the fabrication of the cell trap array.
[0049] The first cell carrier b has a concave array b1 for implanting cells, which provides a controllable space for the implanted cells to grow, preventing the cells from expanding outward in the horizontal direction and improving the consistency of the cell regeneration process. The second cell carrier c encloses and wraps the cells in the concave array b1 to form a cell trap array. Each cell trap on the second cell carrier c is a multi-cell system. Each cell in the system is mutually constrained with other cells, and the temporal and spatial characteristics of its cell signal activation will gradually stabilize, and it has a certain resistance to external disturbances.
[0050] See Figures 11 to 12This utility model also discloses an embodiment of an automated manufacturing system for cell trap arrays based on a human-computer interaction mechanism using a cell carrier membrane scaffold, a carrier fabrication device, and a carrier dehydration device. The system includes a workbench 1, a robotic arm 2, an industrial camera 3, and a control module 4. The workbench 1 is provided with an array fabrication area A and a cell seeding area B. The array fabrication area A includes an array mold 11, a support assembly, and a cell carrier coating scaffold 12. The cell carrier coating scaffold 12 and the support assembly cooperate to form a receiving cavity, in which an inert gel a is placed. The inert gel a is pressed into a concave array b1 by the array mold 11 to form a first cell carrier b. The first cell carrier b can be removed from the support assembly along with the cell carrier coating scaffold 12. The cell inoculation area B includes a dehydration platform 13 and a dehydration module 14. The dehydration module 14 has a dehydration channel 141, and the dehydration platform 13 has a heat source cavity 131 for providing hot air to the dehydration channel 141. The dehydration channel 141 is connected to the heat source cavity 131. The cell carrier coating scaffold 12 carrying the first cell carrier b is detachably connected to the dehydration module 14. An industrial camera 3 is positioned above the cell inoculation area B to perform visual imaging of the concave array b1 in the cell inoculation area B to obtain an image of the position of the concave array b1. The control module 4 is electrically connected to the robotic arm 2 and the industrial camera 3. The drive end of the robotic arm 2 is equipped with a pipette 21. The control module 4 controls the robotic arm 2 to drive the pipette 21 to implant cells into the concave array b1 corresponding to the position image of the concave array b1 based on the position image fed back by the industrial camera 3. After cell seeding and culture, a second membrane scaffold 15 is set outside the first cell carrier b. A second cell carrier c is set inside the second membrane scaffold 15, which is positioned opposite to the concave array b1. The first cell carrier b is at least partially exposed in the dehydration channel 14 for dehydration. The second cell carrier c enters the concave array b1 and encapsulates the cells to form a cell trap array.
[0051] In this embodiment, the workbench 1 is modularly partitioned, with an array fabrication area A and a cell inoculation area B. In the array fabrication area A, an inert gel a and its top concave array b1 are formed by the cooperation of an array mold 11, a support component, and a cell carrier membrane scaffold 12, to obtain a first cell carrier b that can be detached from the support component along with the cell carrier membrane scaffold 12. In the cell inoculation area B, the cell carrier membrane scaffold 12 carrying the first cell carrier b is detachably connected to the dehydration module 14, and the concave array b1 is observed by an industrial camera 3 above the cell inoculation area B. The concave array b1 is subjected to visual imaging to obtain an image of its position. Based on the position image of the concave array b1 fed back by the industrial camera 3, the control module 4 controls the robotic arm 2 to drive the pipette 21 to implant cells into the concave array b1. After the first cell carrier b, along with the cell carrier membrane scaffold 12, is detached from the dehydration module 14, it can be placed in a culture environment for cell culture. The concave array b1 provides a physical boundary for the growth of cell spheres or organoids, allowing for the acquisition of cell spheres or organoids of controllable type, quantity, and density on the first cell carrier b. Once the cells are in... After the first cell carrier b completes self-assembly, the cell carrier membrane scaffold 12 carrying the first cell carrier b is detachably connected to the dehydration module 14, exposing the surface of the first cell carrier b opposite to the concave array b1 to the dehydration channel 141. A second membrane scaffold 15 is placed outside the first cell carrier b, with the second cell carrier c inside the second membrane scaffold 15 positioned opposite to the concave array b1. The second cell carrier c will enter the concave array b1 and encapsulate the cells. Simultaneously, under the action of hot air within the dehydration channel 141, the cells are dehydrated by the first cell carrier b. Rapid dehydration allows the second cell carrier c to easily separate from the first cell carrier b, leaving the cells in the second cell carrier c to form a cell trap array. In this embodiment, a human-machine interactive robotic arm 2, in conjunction with an industrial camera 3, assists the operator's fine movements. At the same time, the structural cooperation between the dehydration platform 13, dehydration module 14, cell carrier membrane scaffold 12, array mold 11, support components, and second membrane scaffold 15 on the workbench 1 simplifies the high-throughput manufacturing process of organoids and improves the controllability and consistency of the organoid regeneration process.
[0052] The cell carrier membrane scaffold 12 can be used in the array fabrication area A for forming the concave array b1, and can also be directly moved to the cell seeding area B as a dehydration carrier, which helps to simplify the fabrication and transfer of the first cell carrier b.
[0053] The heat source in the heat source chamber 131 is provided by an air compressor 5 and a gas heater 6, which are connected in sequence. The air compressor 5 draws air from the environment and pressurizes it to a certain pressure. The pressurized air is heated to a certain temperature by the gas heater 6 and then input into the heat source chamber 131 of the dehydration platform 13 to provide hot convection air for the dehydration module 14. The gas heater 6 includes a cooling module and a heating module to achieve an adjustable temperature. The dehydration channel 141 of the dehydration module 14 is connected to the cooling module and the heating module to provide hot convection air with an adjustable temperature to the dehydration channel 141. The dehydration platform 13 is provided with an air inlet 136 and an air outlet 137 that are connected to the heat source chamber 131. The pressurized air is heated to a certain temperature by the gas heater 6 and then input into the heat source chamber 131 through the air inlet 136.
[0054] The workbench 1 is provided with an installation plate 18 for installing the dehydration platform 13. The installation plate 18 is provided with an assembly groove 181 and a positioning groove 182. The assembly groove 181 is adapted to the dehydration platform 13 and the assembly groove 181 communicates with the positioning groove 182. The positioning groove 182 is provided with an elastic positioning element (not shown in the figure) for elastically abutting against the side wall of the dehydration platform 13 in the assembly groove 181, so as to realize the quick positioning and installation of the dehydration platform 13.
[0055] The dehydration modules 14 on the dehydration platform 13 not only provide dehydration channels 141 during the dehydration process, but also serve to fix the cell carrier membrane scaffold 12 during cell seeding. To achieve higher throughput cell seeding, this embodiment arranges multiple dehydration modules 14 in an array on the dehydration platform 13 in the cell seeding area B, so that multiple cell carrier membrane scaffolds 12 with the first cell carrier b can be installed in the cell seeding area B. Each dehydration module 14 can be individually detached from the dehydration platform 13, making experiments more convenient.
[0056] In addition, the cell seeding area B is equipped with an XY motorized translation stage 19, and a dehydration platform 13 is located on top of the XY motorized translation stage 19. This platform drives the dehydration platform 13 to move laterally and longitudinally, allowing the first cell carriers b on different dehydration modules 14 to fall into the visual range of the industrial camera 3. The robotic arm 2 drives the pipette 21 to implant cells into the concave array b1 on the first cell carriers b that have fallen into the visual range of the industrial camera 3. The cell types in the pipette 21 can be set as needed, and the number of cells implanted in the concave array b1 can be controlled by the pipette 21. Cells can be implanted into concave arrays at different intervals by controlling the robotic arm 2 or the XY motorized translation stage 19, thus controlling the cell density distribution of the concave array b1.
[0057] In this embodiment, the control module 4 uses a computer as the control center of the entire system to process information from various parts and issue control commands.
[0058] In this embodiment, the workbench 1 is modularly distributed. In addition to the array fabrication area A and the cell seeding area B mentioned above, a robotic arm 2 module area and a microscopic vision area are also provided. The robotic arm 2 is located in the robotic arm 2 module area of the workbench 1, and the industrial camera 3 is located in the microscopic vision area of the workbench 1. The modules are connected together by a base plate to form an integral structure.
[0059] The robotic arm 2 is equipped with an electric gripper 22 at its drive end, which holds the pipette 21. The robotic arm 2 can achieve multi-degree-of-freedom, high-speed, and high-precision movements. Working in conjunction with the electric gripper 22, it can grasp objects over a wide range, at high speed, and with high precision, moving them to the appropriate position and enabling human-machine interaction. Using the electric gripper 22 to hold the pipette 21 allows for rapid, accurate, and stable cell seeding.
[0060] The microscopic vision area includes an industrial camera 3, a lens, a light source, and a mounting bracket. The lens and light source are both mounted on the industrial camera 3. The light source's supplementary illumination and the lens's focusing help obtain clearer images. The industrial camera 3 is fixedly mounted on the worktable 1 via the mounting bracket. The industrial camera 3 captures images of the positions of the concave aperture array b1 on the first cell carrier b. After computer image processing, the robotic arm 2 and the XY motorized translation stage 19 are controlled to move accordingly. By translating the XY motorized translation stage 19 along the X and Y directions, the industrial camera 3 can observe the different concave aperture arrays b1 on the first cell carrier b under a microscope.
[0061] In addition to fixing the robotic arm 2 and industrial camera 3 on the workbench 1, the industrial camera 3 can also be fixed on the drive end of the robotic arm 2 to automate recognition and operation through computer vision technology.
[0062] To ensure a clean experimental environment, a biosafety cabinet 7 is also installed on the workbench 1 in this embodiment. The biosafety cabinet 7 is equipped with a working area, a ventilation system, a filtration system, a UV lamp, a protective barrier, etc. The workbench 1 is located in the working area of the biosafety cabinet 7, so that the organoid array can be manufactured in the working area of the biosafety cabinet to minimize contamination.
[0063] In addition, depending on specific needs, equipment modules such as vibrators, centrifuges, and PCR instruments can also be installed on workbench 1.
[0064] The working process of this utility model is as follows: The support 16 is placed in the second culture dish 17. Then, the cell carrier coating scaffold 12 is placed on the corresponding support 16. Agarose is poured into the cell carrier coating scaffold 12, and then the array mold 11 is pressed onto the cell carrier coating scaffold 12. After the agarose solidifies, the array mold 11 is removed, resulting in the first cell carrier b solidified on the cell carrier coating scaffold 12. The cell carrier coating scaffold 12 is placed on the dehydration module 14. Under the visual guidance of the industrial camera 3, the experimenter controls the robotic arm 2 and the electric gripper 22 to grasp the pipette 21 and move it above the dehydration platform 13. By controlling the XY electric translation stage 19, the dehydration module 14 is precisely moved below the pipette 21. Cells are sequentially seeded into the concave array b1 of the first cell carrier b. After seeding, the cell carrier coating scaffold 12 is removed and placed in the second culture dish 17 or a six-well plate for culture, thus rapidly creating a cell array with controllable cell type, quantity, and density. After the cells complete self-assembly in the first cell carrier b, the cell carrier membrane scaffold 12 is removed from the second culture dish 17 or a six-well plate and placed on the dehydration module 14. The second culture dish 17, with a substrate gel evenly coated on the bottom, is then placed over the first cell carrier b. At this time, the substrate gel enters the concave array b1, encapsulating the cultured cell spheres or organoids. Simultaneously, constant-temperature air generated by the air compressor 5 and the gas heater 6 convects with the first cell carrier b, causing the water on the side of the first cell carrier b and the second cell carrier c opposite to the concave array b1 to be quickly removed. The second cell carrier c, made of substrate gel, forms an entangled state during the dehydration process and can be easily physically separated from the first cell carrier b. Therefore, the second culture dish 17 and the second cell carrier c inside it can be easily removed. The cell spheres or organoids in the concave array b1 are then transferred to the second cell carrier c inside the second culture dish 17, thereby rapidly creating an organoid array with controllable cell types, numbers, and densities.
[0065] The above-disclosed embodiment is merely a preferred embodiment of the present utility model and should not be construed as limiting the scope of the present utility model. Therefore, any equivalent variations made in accordance with the claims of the present utility model shall still fall within the scope of the present utility model.
Claims
1. A cell carrier membrane-coated scaffold, characterized in that, The device includes a scaffold body and a first support boss. The scaffold body has a through hole, and the first support boss is arranged circumferentially along the through hole. The first support boss is used to support a first cell carrier.
2. The cell carrier membrane scaffold as described in claim 1, characterized in that, The top surface of the first support boss is at a preset distance from the top of the bracket body.
3. The cell carrier membrane scaffold as described in claim 1 or 2, characterized in that, It also includes support arms, which are arranged in an array along the outer periphery of the support body.
4. A carrier manufacturing apparatus, characterized in that, The invention includes an array mold, a support assembly, and a cell carrier membrane scaffold as described in any one of claims 1 to 3. The membrane scaffold is disposed within the support assembly, which has a second support boss that is adapted to the through hole. The second support boss extends from the bottom through the through hole to form a receiving cavity. An inert gel that contacts the first support boss is disposed within the receiving cavity. The bottom of the array mold has a protrusion array that forms a concave hole array on the top surface of the inert gel.
5. The carrier fabrication apparatus as described in claim 4, characterized in that, The support assembly includes a petri dish and a support, the support being disposed in the petri dish, and the second support boss being disposed on the support.
6. The carrier fabrication apparatus as described in claim 5, characterized in that, The support also includes a base and a limiting boss. The second supporting boss and the limiting boss are both formed on the top of the base. The limiting boss is arranged around the outer periphery of the second supporting boss. The main body of the bracket is located between the limiting boss and the second supporting boss.
7. The carrier fabrication apparatus as described in claim 6, characterized in that, The protrusion array is disposed opposite to the second support protrusion and offset from the first support protrusion.
8. The carrier fabrication apparatus as described in claim 4, characterized in that, The protrusions in the protrusion array are conical.
9. A carrier dehydration device, characterized in that, The device includes a dehydration module and the cell carrier membrane scaffold as described in claim 3. The dehydration module has a dehydration channel and an installation groove arranged in a rotationally symmetrical manner at its end. The installation groove is correspondingly arranged with the support arm. The support arm is screwed into the installation groove, and the scaffold body extends into the dehydration channel.
10. The carrier dehydration apparatus as described in claim 9, characterized in that, The mounting groove is spirally arranged, and the support arm is arranged along the spiral direction of the mounting groove on the outer periphery of the bracket body.