Extraction and concentration device

Abstract

The utility model relates to the technical field of biology, in particular to an extracting and concentrating device which comprises a concentrating cylinder, a filtering mechanism, a communicating pipe, an adapter and a driving mechanism, the top of the concentration cylinder is detachably connected with a mounting cover; the top of the mounting cover is fixedly connected with a mounting box; the filtering mechanism is rotationally arranged in the concentration cylinder, a supporting ring is fixedly connected in the concentration cylinder, and the filtering mechanism is rotationally connected with the concentration cylinder through the supporting ring; the communicating pipe is arranged on the mounting cover, penetrates through the mounting cover, and penetrates and extends into the filtering mechanism; the adapter is arranged in the mounting box, one end of the adapter is communicated with a liquid inlet pipe, and the other end of the adapter is connected with the communicating pipe; and the driving mechanism is arranged on the mounting box. According to the exosome separation and concentration device, the filtering mechanism and the driving mechanism are arranged in a matched mode, an exosome crude extracting solution penetrates through the filtering cylinder in an accelerated mode under the action of centrifugal force, the separation and concentration efficiency of exosomes is greatly improved, and the industrial-grade exosome separation and concentration requirements are met.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology, specifically relates to a kind of extraction concentration device. BACKGROUND

[0002] Exosome is the intracellular body reverse budding formation multicystic endosome, and multicystic endosome is fused with cell membrane, is released to form exosome outside cell. Almost all cells can secrete exosome (diameter 30-100nm), and it exists in most kinds of cells and various body fluids, and also exists in most kinds of cell culture medium. Exosome contains various components, such as protein, lipid, RNA, wherein RNA species has: mRNA, miRNA and other non-coding RNA.

[0003] The Chinese patent with publication number CN213885281U discloses an exosome separation and concentration device, which includes a multi-stage separator, a buffer bottle, a tangential flow concentrator and a collection bottle. Through structural design, the multi-stage separator is used to extract cell exosomes, and the appropriate pore size is selected to separate and filter specific biological structures, and finally the exosome liquid smaller than 150nm is intercepted for concentration. The separation and concentration device designed reasonably, the filter element in the separator can be repeatedly used by flushing with buffer solution, and the tangential flow concentrator can be selected in multiple groups in series or parallel according to the total amount of concentrated liquid, to improve the use activity of the equipment.

[0004] However, the above technical solution has the following disadvantages: the separation and filtration of cell exosomes are realized by connecting multiple separators in series. On the one hand, the whole device has a large volume, occupies a large space and is not convenient to operate. On the other hand, the separation efficiency is low, and it is difficult to meet the large-scale separation and concentration requirements of exosomes. UTILITY MODEL CONTENT

[0005] The utility model aims at the problems in the background art, and provides an extraction concentration device.

[0006] The technical scheme of the utility model: an extraction concentration device, comprising:

[0007] The top of the concentration cylinder is detachably connected with a mounting cover, and the top of the mounting cover is fixedly connected with a mounting box;

[0008] The filter mechanism is rotatably arranged in the concentration cylinder, and the concentration cylinder is fixedly connected with a support ring. The filter mechanism is rotatably connected with the concentration cylinder through the support ring;

[0009] The communication pipe is arranged on the mounting cover, penetrates the mounting cover and is rotatably connected with the mounting cover, and the communication pipe penetrates and extends into the filter mechanism;

[0010] The adapter is arranged in the mounting box, one end of the adapter is communicated with the liquid inlet pipe, the other end of the liquid inlet pipe is communicated with the communicating pipe, and the communicating pipe is connected with the liquid outlet pipe.

[0011] The driving mechanism is arranged on the mounting box, and the output end of the driving mechanism is connected with the communicating pipe for driving the communicating pipe to rotate.

[0012] Preferably, the filtering mechanism comprises a mounting seat, a sealing cover and a plurality of filter cartridges, the mounting seat is rotatably connected with the supporting ring, the filter cartridges are fixedly connected with the mounting seat at bottom ends thereof, the filter cartridges are fixedly connected with the sealing cover at top ends thereof, and the sealing cover is provided with a communicating hole communicated with the communicating pipe.

[0013] Preferably, the adapter comprises a mounting cylinder, a connecting head and a connecting seat, the top end of the mounting cylinder is fixedly connected with the top of the inner wall of the mounting box, the connecting head is rotatably arranged in the mounting cylinder, the communicating pipe extends into the mounting cylinder and is fixedly connected with the connecting head, the connecting seat is slidably arranged in the mounting cylinder, the liquid inlet pipe extends into the mounting cylinder and is fixedly connected with the connecting seat, and the communicating pipe and the liquid inlet pipe are communicated through the connecting head and the connecting seat.

[0014] Preferably, a plurality of sealing springs are arranged, one end of each of the sealing springs is connected with the connecting seat, and the other end of each of the sealing springs is connected with the inner wall of the mounting cylinder.

[0015] Preferably, the driving mechanism comprises a driving motor, a driving rod, a driving wheel and a driven wheel, the driving motor is fixedly connected with the mounting box, the driving rod is fixedly connected with the output end of the driving motor, the driving wheel is fixedly connected with the driving rod, the driven wheel is fixedly connected with the communicating pipe, and the driving wheel and the driven wheel are meshed with each other.

[0016] Preferably, the bottom of the concentrating cylinder is provided with a plurality of supporting legs, the supporting legs are arranged at equal intervals, and the bottom of the concentrating cylinder is communicated with a collecting pipe.

[0017] Compared with the prior art, the above technical scheme of the utility model has the following beneficial technical effects:

[0018] The utility model discloses a filtering mechanism and driving mechanism cooperate, the process of separating and concentrating exosome by filter cartridge, driving mechanism drives filtering mechanism to rotate, exosome crude extract liquid accelerates through filter cartridge under the action of centrifugal force, greatly improve the separation and concentration efficiency of exosome, meet the demand of separating and concentrating exosome of industrial grade, realize the continuity of separating and concentrating exosome crude extract liquid through the cooperation of adapter, liquid inlet pipe and communicating pipe, further improve the separation and concentration efficiency of exosome. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a perspective view of an embodiment of the utility model.

[0020] Figure 2 A sectional view of an embodiment of the utility model is provided;

[0021] Figure 3 A perspective view of the driving mechanism in an embodiment of the utility model is provided;

[0022] Figure 4 A perspective view of the connecting seat in an embodiment of the utility model is provided;

[0023] Figure 5 A perspective view of the connecting head in an embodiment of the utility model is provided;

[0024] Figure 6 A perspective view of the supporting ring in an embodiment of the utility model is provided.

[0025] The drawing reference: 1, the thickening cylinder; 2, the installation cover; 31, the mounting seat; 32, the sealing cover; 33, the filter cylinder; 41, the installation cylinder; 42, the connecting head; 43, the connecting seat; 44, the sealing spring; 51, the driving motor; 52, the driving rod; 53, the driving wheel; 54, the driven wheel; 6, the installation box; 7, the supporting ring; 8, the communication pipe; 9, the liquid inlet pipe; 10, the supporting leg; 11, the collection pipe. Specific implementation

[0026] Embodiment one

[0027] As Figures 1-6 shown, the utility model provides an extraction thickening device, including thickening cylinder 1, filter mechanism, communication pipe 8, adapter and driving mechanism;

[0028] Thickening cylinder 1 top detachably connected with installation cover 2, and installation cover 2 with thickening cylinder 1 between through multiple buckles snap fixed, installation cover 2 top fixedly connected with installation box 6, thickening cylinder 1 bottom is provided with multiple supporting legs 10, and supporting leg 10 is provided with four and equidistantly sets up, and the bottom of thickening cylinder 1 is communicated with collection pipe 11;

[0029] Filter mechanism rotation is set up in thickening cylinder 1, and thickening cylinder 1 is fixedly connected with supporting ring 7, and filter mechanism is rotationally connected with thickening cylinder 1 through supporting ring 7;

[0030] Communication pipe 8 is set up on installation cover 2, and communication pipe 8 penetrates installation cover 2 and is rotationally connected with installation cover 2, and communication pipe 8 penetrates and extends to filter mechanism inside;

[0031] Adapter is set up in installation box 6, and the one end of adapter is communicated with liquid inlet pipe 9, and the one end of liquid inlet pipe 9 away from adapter penetrates installation box 6 and extends to the outside of installation box 6, and the other end of adapter is connected with communication pipe 8;

[0032] A driving mechanism is arranged on the mounting box 6, and an output end of the driving mechanism is connected with the communicating pipe 8 for driving the communicating pipe 8 to rotate.

[0033] Embodiment two

[0034] As Figure 2 shown, the utility model discloses an extraction concentration device, compared with embodiment one, this embodiment still specifically disclosed the structure of filter mechanism, filter mechanism includes mounting seat 31, sealing cover 32 and filter cartridge 33, mounting seat 31 is connected with the rotation of support ring 7, filter cartridge 33 is provided with a plurality of, from inside to outside filter cartridge 33's filter hole is smaller and smaller, the bottom of filter cartridge 33 is fixedly connected with mounting seat 31, the top of filter cartridge 33 is fixedly connected with sealing cover 32, sealing cover 32 is passed through and is opened with the communicating hole of communicating with pipe 8 adaptation, communicating pipe 8 extends to the inside of sealing mechanism through the communicating hole, only along the axis direction of communicating pipe 8 sliding between communicating pipe 8 and sealing cover 32.

[0035] Embodiment three

[0036] As Figures 2-5 shown, the utility model discloses an extraction concentration device, compared with embodiment two, this embodiment still specifically disclosed the structure of adapter, adapter includes installation cylinder 41, connecting head 42 and connecting seat 43, the top of installation cylinder 41 is fixedly connected with the top of mounting box 6 inner wall, connecting head 42 rotationally arranged in installation cylinder 41, communicating pipe 8 extends to installation cylinder 41 and is fixedly connected with connecting head 42, connecting seat 43 is slidably arranged in installation cylinder 41, inlet pipe 9 extends to installation cylinder 41 and is fixedly connected with connecting seat 43, communicating pipe 8 and inlet pipe 9 are communicated through connecting head 42 and connecting seat 43, still include a plurality of sealing spring 44, one end of sealing spring 44 is connected with connecting seat 43, the other end of sealing spring 44 is connected with the inner wall of installation cylinder 41.

[0037] Embodiment four

[0038] As Figures 2-3 shown, the utility model discloses an extraction concentration device, compared with embodiment three, this embodiment still specifically disclosed the structure of driving mechanism, driving mechanism includes drive motor 51, drive rod 52, driving wheel 53 and driven wheel 54, drive motor 51 is fixedly connected with mounting box 6, drive motor 51 has the function of positive and negative rotation, is connected with control system and power supply through wire, drive rod 52 is fixedly connected with the output end of drive motor 51, driving wheel 53 is fixedly connected with drive rod 52, driven wheel 54 is fixedly connected with communicating pipe 8, and the intermeshing of driving wheel 53 and driven wheel 54.

[0039] The driving motor 51 is turned on through the control panel, and the driving motor 51 works to drive the driving shaft 52 to rotate the driving wheel 53, the driving wheel 53 rotates to drive the driven wheel 54 and the communication pipe 8 to rotate through the meshing effect, the communication pipe 8 rotates to drive the filtering mechanism to rotate, and the exosome crude extract is guided into the filtering mechanism in the concentration cylinder 1 through the liquid inlet pipe 9, the adapter and the communication pipe 8, and the exosome crude extract is discharged from the collection pipe 11 out of the concentration cylinder 1 after passing through the filtering cylinder 33 under the action of the centrifugal force.

[0040] The above describes the embodiments of the utility model in detail in combination with the drawings, but the utility model is not limited to this, and various changes can be made within the knowledge range of the technical personnel in the technical field without departing from the purpose of the utility model.

Claims

1. An extraction concentration apparatus, characterized by, The utility model relates to a kind of liquid concentration device, including: Concentrated cylinder (1), its top detachably connected with installation cover (2), installation cover (2) top fixedly connected with installation box (6); Filter mechanism is rotationally arranged in concentrated cylinder (1), and support ring (7) is fixedly connected in concentrated cylinder (1), and filter mechanism is rotationally connected with concentrated cylinder (1) by support ring (7); Communication pipe (8) is arranged on installation cover (2), and communication pipe (8) penetrates installation cover (2) and is rotationally connected with installation cover (2), and communication pipe (8) extends to filter mechanism; Adapter is arranged in installation box (6), one end of adapter is communicated with liquid inlet pipe (9), the end of liquid inlet pipe (9) away from adapter penetrates installation box (6) and extends to the outside of installation box (6), the other end of adapter is connected with communication pipe (8); Driving mechanism is arranged on installation box (6), and the output end of driving mechanism is connected with communication pipe (8) for driving communication pipe (8) rotation.

2. The extraction concentration device according to claim 1, wherein Filter mechanism includes mounting seat (31), sealing cover (32) and filter cylinder (33);Mounting seat (31) is rotationally connected with support ring (7), and a plurality of filter cylinders (33) are provided, and the bottom end of filter cylinder (33) is fixedly connected with mounting seat (31), and the top end of filter cylinder (33) is fixedly connected with sealing cover (32), and sealing cover (32) is penetrated and is communicated with the communication hole of communication pipe (8) matched.

3. The extraction concentration device of claim 1, wherein Adapter includes mounting cylinder (41), connecting head (42) and connecting seat (43);The top end of mounting cylinder (41) is fixedly connected with the top of the inner wall of installation box (6), connecting head (42) is rotationally arranged in mounting cylinder (41), and communication pipe (8) extends to mounting cylinder (41) and is fixedly connected with connecting head (42), connecting seat (43) is slidably arranged in mounting cylinder (41), and liquid inlet pipe (9) extends to mounting cylinder (41) and is fixedly connected with connecting seat (43), and communication pipe (8) and liquid inlet pipe (9) are communicated by connecting head (42) and connecting seat (43).

4. The extraction concentration device of claim 3, wherein Still include a plurality of sealing springs (44), one end of sealing spring (44) is connected with connecting seat (43), and the other end of sealing spring (44) is connected with the inner wall of mounting cylinder (41).

5. The extraction concentration device of claim 1, wherein Driving mechanism includes driving motor (51), drive rod (52), driving wheel (53) and driven wheel (54);Driving motor (51) is fixedly connected with installation box (6), drive rod (52) is fixedly connected with the output end of driving motor (51), driving wheel (53) is fixedly connected with drive rod (52), and driven wheel (54) is fixedly connected with communication pipe (8), and driving wheel (53) and driven wheel (54) are engaged with each other.

6. The extraction concentration device of claim 1, wherein Concentrated cylinder (1) bottom is provided with a plurality of support legs (10), and the support leg (10) is provided with four and is equidistantly arranged, and the bottom of concentrated cylinder (1) is communicated with collecting pipe (11).

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