Leakage-proof cryopreservation bag

Abstract

The utility model discloses a leakproof cryopreservation bag which comprises a bag body, an outer sleeve and an infusion device, a liquid inlet is formed in the bag body, a first valve is arranged in the liquid inlet of the bag body, the first valve is a revolving body, the first valve and the bag body are integrally formed, a cross-shaped incision A is formed in the first valve, and the cross-shaped incision A and the first valve are concentric; the outer sleeve is inserted into the liquid inlet of the bag body, a gap exists between the outer sleeve and the first valve, and the bag body is in sealed connection with the outer sleeve in a heat sealing mode; the infusion device comprises a puncture tube used for penetrating through the first valve to input a sample into the bag body, and the end, exposed out of the bag body, of the puncture tube is connected with an infusion connector. The puncture tube of the infusion device can penetrate through the first valve to input a sample into the bag body, and when a heat sealing position is poor in sealing and has a gap, the first valve in the liquid inlet of the bag body can be used for preventing the sample in the bag body from leaking out of the liquid inlet, so that the sample in the bag body is prevented from being lost.

Description

Technical Field

[0001] This utility model belongs to the field of medical devices, and more specifically, relates to a leak-proof cryopreservation bag. Background Technology

[0002] The main function of cryopreservation bags is for the long-term storage and transport of biological products such as blood, cells, vaccines, and viruses at low temperatures. They are made of medical-grade EVA cryogenic material, possessing excellent sealing and protective properties, effectively preventing the ingress of external air and moisture to ensure that samples maintain their original quality and characteristics during freezing and thawing.

[0003] The specific applications of cryopreservation bags are as follows: 1. Stem cell storage: Cryopreservation bags are used to store stem cells, ensuring that they are preserved at low temperatures for extended periods to maintain their bioactivity. 2. Umbilical cord blood storage: Used for storing umbilical cord blood, ensuring that hematopoietic stem cells in the cord blood are preserved at low temperatures for future use. 3. Scientific research: In the scientific research field, cryopreservation bags are widely used to preserve experimental samples such as cells, DNA, and RNA, ensuring the stability and reliability of the samples during the freezing process. 4. Biomedical field: Used for the preservation and transportation of drugs and vaccines, ensuring that the active ingredients of drugs are not lost and that vaccines remain safe and effective during transportation. 5. Chemical industry: Used for the preservation and transportation of chemical reagents, protecting the purity and activity of reagents and reducing loss and contamination.

[0004] Cryopreservation bags should maintain good sealing and protective properties to prevent external liquids from entering and contaminating the samples, protect the sterility of the samples, and reduce the risk of cross-contamination.

[0005] Existing cryopreservation bags generally consist of a bag body for holding samples and an infusion main pipe heat-sealed to the bag body for infusion, with three branch pipes connected to the main pipe. Because cryopreservation bags are first frozen and then heated to thaw, pressure and temperature changes occur during freezing and heating. If the heat seal between the infusion main pipe and the bag body is of poor quality and the connection is not secure, gaps can easily appear, causing the sample inside the bag to leak out and resulting in sample loss. This is especially problematic when the cryopreservation bag is directly placed in warm water for heating, as warm water can enter the cryopreservation bag, contaminating the sample and rendering it unusable. Utility Model Content

[0006] To address the aforementioned deficiencies or improvement needs of existing technologies, this utility model provides a leak-proof cryopreservation bag. It uses a puncture tube to pass through the first valve before the sample is introduced, allowing the sample to be properly introduced into the bag. The bag's inlet uses a valve structure to effectively prevent the sample from leaking out.

[0007] To achieve the above objectives, according to this utility model, a leak-proof cryopreservation bag is provided, comprising a bag body, an outer tube, and an infusion device, wherein the bag body is provided with an inlet, characterized in that:

[0008] The inlet of the bag is provided with a first valve to prevent the sample inside the bag from leaking out of the inlet. The material of the first valve is the same as that of the bag. The first valve is a rotating body and is integrally formed with the bag. The first valve is provided with a cross-shaped cut A and the cross-shaped cut A is concentric with the first valve.

[0009] The outer tube is inserted into the liquid inlet of the bag body, and there is a gap between the outer tube and the first valve. The bag body is sealed to the outer tube by heat sealing.

[0010] The infusion device includes a puncture tube for passing through the first valve to introduce a sample into the bag, with one end of the puncture tube exposed in the bag connected to an infusion connector.

[0011] Preferably, the infusion device further includes a needle core detachably mounted on the puncture tube, the puncture tube enclosing the needle core.

[0012] Preferably, the puncture tube is made of medical-grade silicone or medical-grade polyurethane.

[0013] Preferably, the first valve is cone-shaped or spherical.

[0014] Preferably, the end of the outer sheath away from the first valve is provided with a Luer connector.

[0015] Preferably, a second valve is integrally formed on the outer tube to prevent external liquid from entering the bag and contaminating the sample. The material of the second valve is the same as that of the outer tube. The second valve is a rotating body and is integrally formed with the outer tube. A cross-shaped incision B is provided on the second valve, and the cross-shaped incision B is concentric with the second valve. The puncture tube passes through the second valve.

[0016] Preferably, the second valve is cone-shaped or spherical.

[0017] Preferably, the distance between the end of the outer sleeve extending into the liquid inlet and the first valve is 3mm to 6mm.

[0018] Preferably, the bag body is provided with multiple butterfly nozzles to facilitate the removal of the sample from the bag body after the butterfly nozzles are broken off.

[0019] Preferably, the second valve is positioned within the outer tube not beyond the butterfly mouth.

[0020] In summary, compared with the prior art, the above-described technical solution conceived by this utility model can achieve the following beneficial effects:

[0021] 1) The present invention provides a leak-proof cryopreservation bag, which can hold liquid samples. The puncture tube of the infusion device can pass through the first valve to introduce the sample into the bag. The bag is sealed to the outer tube by heat sealing, which can play a good sealing role. When the heat sealing is not good and there is a gap, the first valve in the liquid inlet of the bag can be used to prevent the sample in the bag from leaking out of the liquid inlet, thus preventing the sample from leaking out of the bag and causing sample loss.

[0022] 2) The leak-proof cryopreservation bag of this utility model has a second valve integrally formed on the outer tube. The second valve can prevent external liquid from entering the bag and contaminating the sample, thereby achieving a good protective effect. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of this utility model. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0025] Reference Figure 1 A leak-proof cryopreservation bag includes a bag body 1, an outer tube 2, and an infusion device 3, which can hold samples. The bag body 1 is provided with an inlet 11. The bag body 1 of this utility model is the same as the bag body 1 of a conventional cryopreservation bag. Its perimeter is sealed, but only an inlet 11 is left to facilitate the input of samples. The input sample is a liquid.

[0026] A first valve 4 is provided inside the liquid inlet 11 of the bag body 1 to prevent the sample inside the bag body 1 from leaking out of the liquid inlet 11. The material of the first valve 4 is the same as that of the bag body 1. The first valve 4 is a rotating body, preferably conical or spherical in shape, and is integrally formed with the bag body 1. The first valve 4 has a cross-shaped cut A, which is concentric with the first valve 4, i.e., their centers are the same. The structure and working principle of the first valve 4 can be referenced from the valve with a cross-shaped cut in the sippy cup of an infant. The first valve 4 of this utility model is mainly used to effectively prevent the sample inside the bag body 1 from leaking out when a gap appears due to separation between the bag body 1 and the outer tube 2 at the heat seal. It also plays a certain role in preventing external hot water from entering the bag body 1 when the cryopreservation bag is heated.

[0027] The outer tube 2 is inserted into the liquid inlet 11 of the bag body 1, and there is a gap between the outer tube 2 and the first valve 4. The bag body 1 is sealed to the outer tube 2 by heat sealing. This gap between the outer tube 2 and the first valve 4 ensures that the structure of the first valve 4 will not be damaged during heat sealing, and facilitates heat sealing between the bag body 1 and the outer tube 2.

[0028] The infusion device 3 includes a puncture tube 31 for passing through the first valve 4 to introduce a sample into the bag body 1. One end of the puncture tube 31, protruding from the bag body 1, is connected to an infusion connector 32. The infusion connector 32 connects to an external liquid storage structure. Liquid in the storage structure passes sequentially through the infusion connector 32 and the puncture tube 31 before entering the cryopreservation bag for storage. Because of the first valve 4, this invention cannot directly introduce liquid into the bag body 1 of a conventional cryopreservation bag through the outer tube 2. Instead, it requires the use of the infusion connector 32 and the puncture tube 31 of the infusion device 3 for infusion. The outer tube 2 can be transparent, while the puncture tube 31 can be colored, such as blue, for easy observation and differentiation. After the liquid is injected into the bag body 1 of the cryopreservation bag through the puncture tube 31, the puncture tube 31 of the infusion device 3 can be manually pulled out from inside the outer tube 2.

[0029] The structure of the infusion device 3 can be referenced to that of a conventional indwelling needle. The structure of the puncture tube 31 of this invention is referenced to the outer sheath of the indwelling needle core. The structure of the infusion connector 32 of this invention is referenced to the connector that connects to the sheath of the indwelling needle. The length of the puncture tube 31 of this invention is slightly longer than the length of the indwelling needle sheath. The infusion connector 32 and the puncture tube 31 can be directly connected together. The infusion connector 32 can be connected to an external structure for providing samples, and the sample flows into the puncture tube 31 after passing through the infusion connector 32. Alternatively, referring to the structure of a conventional indwelling needle, the infusion connector 32 can be connected to another connector via a flexible tube, and the other connector is connected to the structure for providing samples. The puncture tube 31 is preferably made of medical-grade silicone or medical-grade polyurethane, which has good flexibility and facilitates its passage through the first valve 4.

[0030] Furthermore, the infusion device 3 also includes a needle core (not shown in the figure) detachably mounted on the puncture tube 31, with the puncture tube 31 enclosing the needle core. The needle core of this invention can refer to the steel needle of a conventional indwelling needle. The length of the needle core should match the length of the puncture tube 31 to assist the puncture tube 31 in passing through the first valve 4, after which the needle core can be removed.

[0031] As a preferred embodiment, a Luer connector (not shown in the figure) is provided at the end of the outer tube 2 away from the first valve 4. When the puncture tube 31 needs to be inserted into the outer tube 2, the female connector of the Luer connector can be removed. After the puncture tube 31 has finished infusing liquid into the bag 1, the infusion device 3 is manually pulled out from the outer tube 2, and then the female connector of the Luer connector is reattached to seal the cryopreservation bag. The Luer connector has a good sealing effect and can effectively prevent external impurities from entering the outer tube 2 and the bag 11.

[0032] If a Luer connector is not installed, after the sample is introduced into the bag body 1, the infusion device is removed, and then the outer tube 2 is heat-sealed to form a heat-sealing point, thereby sealing the outer tube 2 and the bag body 1.

[0033] Furthermore, a second valve 5 is integrally formed on the outer tube 2 to prevent external liquid from entering the bag 1 and contaminating the sample. The material of the second valve 5 is the same as that of the outer tube 2. The second valve 5 is a rotating body. The second valve 5 is preferably conical or spherical in shape. The second valve 5 is integrally formed with the outer tube 2. A cross-shaped incision B is provided on the second valve 5, and the cross-shaped incision B is concentric with the second valve 5, that is, the centers of the two are the same. The puncture tube 31 also passes through the second valve 5. Therefore, the puncture tube 31 passes through the second valve 5 first and then through the first valve 4.

[0034] The second valve 5, together with the Luer connector, can provide excellent protection for the sample inside the bag 1.

[0035] If the heat-sealed point on the outer tube 2 becomes loose and has a gap, or if the Luer connector is not firmly bonded to the outer tube 2 and has a gap, the second valve 5 can effectively prevent warm water from entering the body 1 of the cryopreservation bag and contaminating the sample when the cryopreservation bag is heated in a water bath.

[0036] Furthermore, the distance between the end of the outer sleeve 2 that extends into the liquid inlet 11 and the first valve 4 is 3mm to 6mm, to prevent the two from being too close and affecting the heat sealing between the outer sleeve 2 and the bag body 1.

[0037] Furthermore, the bag body 1 is provided with multiple butterfly nozzles 12 to facilitate the removal of the sample from the bag body after the butterfly nozzles are broken off. When it is necessary to remove the sample, one of the butterfly nozzles 12 on the bag body 1 is broken off, and the external plastic puncture needle is forcefully inserted through the butterfly nozzle 12 and into the bag body 1 to remove the sample.

[0038] Furthermore, the second valve 5 is positioned within the outer sleeve 2 without extending beyond the butterfly nozzle 12. The second valve 5 is positioned as close as possible to the liquid inlet 11 to provide good protection. It also facilitates the subsequent heat sealing of the outer sleeve 2 to form a heat seal point. After the outer sleeve 2 is cut off near the heat seal point, the remaining outer sleeve 2 will not extend beyond the butterfly nozzle 12.

[0039] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A leak-proof cryopreservation bag, comprising a bag body (1), an outer tube (2), and an infusion device (3), wherein the bag body (1) is provided with an inlet (11), characterized in that: The inlet (11) of the bag body (1) is provided with a first valve (4) to prevent the sample in the bag body (1) from leaking out of the inlet (11). The material of the first valve (4) is the same as that of the bag body (1). The first valve (4) is a rotating body and is integrally formed with the bag body (1). The first valve (4) is provided with a cross-shaped cut A and the cross-shaped cut A is concentric with the first valve (4). The outer tube (2) is inserted into the liquid inlet (11) of the bag body (1), and there is a gap between the outer tube (2) and the first valve (4). The bag body (1) is sealed to the outer tube (2) by heat sealing. The infusion device (3) includes a puncture tube (31) for passing through the first valve (4) to input a sample into the bag body (1), and one end of the puncture tube (31) exposed outside the bag body (1) is connected to an infusion connector (32).

2. The leak-proof cryopreservation bag according to claim 1, characterized in that, The infusion device (3) also includes a needle core that is detachably mounted on the puncture tube (31), the puncture tube (31) enclosing the needle core.

3. The leak-proof cryopreservation bag according to claim 1, characterized in that, The puncture tube (31) is made of medical silicone or medical polyurethane.

4. The leak-proof cryopreservation bag according to claim 1, characterized in that, The first valve (4) is cone-shaped or spherical.

5. A leak-proof cryopreservation bag according to claim 1, characterized in that, The outer sheath (2) is provided with a Luer connector at the end away from the first valve (4).

6. A leak-proof cryopreservation bag according to claim 1, characterized in that, The outer tube (2) is also integrally formed with a second valve (5) to prevent external liquid from entering the bag (1) and contaminating the sample. The material of the second valve (5) is the same as that of the outer tube (2). The second valve (5) is a rotating body and is integrally formed with the outer tube (2). The second valve (5) is provided with a cross-shaped incision B, and the cross-shaped incision B is concentric with the second valve (5). The puncture tube (31) passes through the second valve (5).

7. A leak-proof cryopreservation bag according to claim 6, characterized in that, The second valve (5) is cone-shaped or spherical.

8. A leak-proof cryopreservation bag according to claim 1, characterized in that, The distance between the end of the outer sleeve (2) that extends into the liquid inlet (11) and the first valve (4) is 3mm to 6mm.

9. A leak-proof cryopreservation bag according to claim 6, characterized in that, The bag (1) is provided with multiple butterfly mouths (12) so that the sample inside the bag (1) can be taken out after the butterfly mouths (12) are broken off.

10. A leak-proof cryopreservation bag according to claim 9, characterized in that, The second valve (5) is positioned inside the outer tube (2) without extending beyond the butterfly mouth (12).

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